Affinage

PDLIM1

PDZ and LIM domain protein 1 · UniProt O00151

Length
329 aa
Mass
36.1 kDa
Annotated
2026-06-10
49 papers in source corpus 24 papers cited in narrative 24 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PDLIM1 (CLP-36/hCLIM1) is a PDZ-LIM scaffold protein that organizes the actin cytoskeleton and couples it to signaling by tethering alpha-actinin and its associated partners onto actin stress fibers (PMID:10753915, PMID:11110697). Its bipartite architecture underlies this adapter role: the N-terminal PDZ domain plus an intervening sequence binds the spectrin-like repeats of nonmuscle alpha-actinin-1 and alpha-actinin-4 and targets the protein to stress fibers (while the LIM domain alone does not), and the C-terminal LIM domain recruits additional clients such as the Clik1 kinase to those actin structures (PMID:10753915, PMID:11110697, PMID:11973348). Through these interactions PDLIM1 builds higher-order complexes with palladin and other actin regulators and is itself required for stress fiber and focal adhesion assembly (PMID:17964547, PMID:19366376, PMID:22659164). Beyond structural scaffolding, PDLIM1 controls Rho-family and Hippo signaling at the actin interface: the alpha-actinin-4 complex governs RhoA activity and traction force generation (PMID:21680739), the alpha-actinin complex drives Cdc42 activation and directional cell migration (PMID:24662836), and competitive binding of alpha-actinin-4 to limit F-actin overgrowth restrains LATS1 dephosphorylation and YAP activation (PMID:31509262). PDLIM1 additionally functions as a cytoplasmic sequestering hub for transcription factors, retaining NF-kB p65 in the cytoplasm in an alpha-actinin-4-dependent manner to suppress inflammation (PMID:26679095) and stabilizing the E-cadherin/beta-catenin junctional complex to block beta-catenin nuclear activity and EMT (PMID:26701804). PDLIM1 abundance is set by regulated degradation, including Cblb-mediated K244 ubiquitination and proteasomal turnover that releases NF-kB-driven pyroptosis (PMID:40696327) and ATG7-dependent autophagic clearance required for cytoskeletal remodeling during spermiogenesis (PMID:27310465). In platelets, PDLIM1 acts as a major negative regulator of GPVI/ITAM signaling, limiting thrombus formation (PMID:22955732).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 2000 High

    Established the founding function of PDLIM1 as a stress-fiber adapter, defining how a PDZ-LIM protein is targeted to the actin cytoskeleton through alpha-actinin binding.

    Evidence Co-IP with MALDI-TOF MS, yeast two-hybrid domain mapping, and GFP-fusion localization in nonmuscle, endothelial, and platelet cells

    PMID:10753915 PMID:10861853 PMID:11110697

    Open questions at the time
    • Did not establish whether actin association is direct or fully alpha-actinin-mediated
    • Functional consequence of stress-fiber targeting not yet tested
  2. 2002 Medium

    Showed PDLIM1 is not just a structural anchor but recruits signaling enzymes to actin, redistributing the otherwise nuclear Clik1 kinase to stress fibers via its LIM domain.

    Evidence Yeast two-hybrid and colocalization with domain-deletion analysis

    PMID:11973348

    Open questions at the time
    • Clik1 substrates on stress fibers unidentified
    • Functional output of LIM-mediated kinase recruitment untested
  3. 2007 Medium

    Demonstrated PDLIM1 is functionally required, not merely associated, for actin stress fiber and focal adhesion assembly, and that both PDZ and LIM domains are essential.

    Evidence RNAi knockdown with domain-deletion rescue in BeWo cells

    PMID:17964547

    Open questions at the time
    • Molecular step in assembly that PDLIM1 controls unresolved
    • Cell-type generality of the requirement not established here
  4. 2007 High

    Extended the scaffold concept to membrane-cytoskeleton coupling by showing PDLIM1 tethers the PMCA calcium pump to the actin/alpha-actinin network in platelets.

    Evidence Co-IP, LC-MS/MS, GST pulldown, gel filtration of a ~1,000 kDa complex, and colocalization

    PMID:17393022

    Open questions at the time
    • Functional consequence for calcium handling not directly measured
    • Stoichiometry of the megacomplex undefined
  5. 2009 Medium

    Identified palladin as a PDZ-domain partner and placed PDLIM1 in an alpha-actinin/palladin complex with mutual dependence for stress-fiber localization, including in neurons where PDLIM1 negatively regulates neurite outgrowth.

    Evidence Y2H, Co-IP, siRNA, and domain-overexpression microscopy in fibroblasts and DRG neurons

    PMID:19366376 PMID:19780892 PMID:20381583

    Open questions at the time
    • Signaling pathway linking the complex to outgrowth suppression unknown
    • Relevance of nerve-injury upregulation to regeneration not functionally tested
  6. 2011 High

    Linked the PDLIM1/alpha-actinin-4 complex to active cytoskeletal mechanics and disease, showing it controls RhoA activity and traction force and is disrupted by FSGS-causing alpha-actinin-4 mutations.

    Evidence Co-IP, siRNA, disease-mutant analysis, RhoA assay, and traction force microscopy in podocytes; TAP-MS complex definition in cardiac tissue

    PMID:21246116 PMID:21680739

    Open questions at the time
    • Mechanism by which the complex regulates RhoA not defined
    • Whether reduced PDLIM1 levels are cause or consequence of complex loss unclear
  7. 2012 High

    Defined PDLIM1's intrinsic biochemistry (femtomolar dual-zinc LIM domain stabilized by C-terminal interactions) and revealed a genetically validated negative-regulatory role in platelet GPVI/ITAM signaling and thrombosis.

    Evidence Biophysical zinc-affinity measurements in vitro; knockout and LIM-truncation mouse models with platelet functional assays and in vivo thrombosis; plus redundancy analysis with RIL on stress fibers

    PMID:22659164 PMID:22922308 PMID:22955732

    Open questions at the time
    • Molecular target of PDLIM1 in the GPVI cascade not identified
    • Extent of functional redundancy with other ALP/Enigma members in vivo unknown
  8. 2014 High

    Established PDLIM1 as a pro-migratory effector in cancer that drives Cdc42 activation, cell polarization, and metastasis strictly through its alpha-actinin interaction.

    Evidence Binding-interface mutagenesis, Cdc42 activity assay, migration/invasion assays, and in vivo metastasis in breast cancer cells

    PMID:24662836

    Open questions at the time
    • Link from the actin complex to Cdc42 GEF/GAP regulation undefined
    • Context-dependence versus tumor-suppressive roles in other cancers unreconciled
  9. 2015 High

    Revealed PDLIM1 as a cytoplasmic sequestering hub controlling transcription-factor localization—stabilizing E-cadherin/beta-catenin junctions to block EMT and retaining NF-kB p65 in the cytoplasm to suppress inflammation.

    Evidence Co-IP, knockdown/overexpression, reporter assays, subcellular fractionation, cytokine ELISA, and in vivo metastasis models; plus PDZ-mediated p75NTR binding driving glioma invasion

    PMID:26119933 PMID:26679095 PMID:26701804

    Open questions at the time
    • How a single scaffold reconciles tumor-suppressive (EMT/NF-kB) and tumor-promoting (p75NTR) outputs unresolved
    • Whether p65 sequestration is direct or alpha-actinin-4-bridged not fully dissected
  10. 2016 Medium

    Showed PDLIM1 abundance must be actively lowered for cytoskeletal remodeling, with ATG7-dependent autophagic degradation required during spermatid differentiation.

    Evidence atg7-null conditional knockout mice with western blot and sperm morphology/motility phenotyping

    PMID:27310465

    Open questions at the time
    • Autophagy receptor selecting PDLIM1 unidentified
    • Whether PDLIM1 accumulation is sufficient to cause the spermiogenesis defect not isolated
  11. 2020 High

    Provided a mechanistic link from PDLIM1 to Hippo signaling, showing competitive sequestration of alpha-actinin-4 from F-actin (via residue N145) limits F-actin overgrowth, preserving LATS1 phosphorylation and restraining YAP-driven HCC metastasis; also placed PDLIM1 at pathogen-induced actin structures.

    Evidence N145A mutagenesis, Co-IP, F-actin binding and LATS1 phosphorylation/YAP assays with in vivo metastasis; immunofluorescence at Listeria/EPEC actin structures with Tir-mutant analysis

    PMID:31509262 PMID:33022122

    Open questions at the time
    • How F-actin level changes are transduced to the LATS1 kinase cassette not detailed
    • Functional role of PDLIM1 at bacterial actin structures untested
  12. 2022 Medium

    Identified a second, opposing route by which PDLIM1 controls YAP—protecting YAP1 from ITCH/AIP-4-dependent proteasomal degradation to promote p53-deficient sarcoma.

    Evidence Co-IP, ubiquitination assay, and CLP36-knockout mouse tumorigenesis model

    PMID:35836803

    Open questions at the time
    • How PDLIM1 shields YAP1 from ITCH mechanistically unclear
    • Reconciliation with the F-actin/LATS1 route to YAP regulation not addressed
  13. 2023 Low

    Suggested a metabolic interaction in which PDLIM1 binds hexokinase 2 to influence glycolysis under glucose deprivation via Wnt/beta-catenin signaling.

    Evidence Single Co-IP with glycolysis assays and knockdown/overexpression in gastric cancer cells

    PMID:37930472

    Open questions at the time
    • Single Co-IP without reciprocal or orthogonal validation
    • Direct versus indirect HK2 association undetermined
    • Mechanistic link to Wnt/beta-catenin not established
  14. 2025 Medium

    Defined how PDLIM1 protein levels are set by the ubiquitin-proteasome system, identifying Cblb as the E3 ligase ubiquitinating K244, with degradation releasing NF-kB-driven microglial pyroptosis.

    Evidence Mass spectrometry, Co-IP, K244 site-specific ubiquitination mapping, proteasome inhibition, and in vivo shRNA/AAV rescue

    PMID:40696327

    Open questions at the time
    • Signals controlling Cblb-PDLIM1 engagement unknown
    • Whether NF-kB release reflects loss of p65 sequestration not directly connected to the 2015 mechanism

Open questions

Synthesis pass · forward-looking unresolved questions
  • How PDLIM1 toggles between tumor-suppressive (E-cadherin/beta-catenin stabilization, NF-kB sequestration, F-actin/LATS1-mediated YAP restraint) and tumor-promoting (Cdc42 migration, YAP1 stabilization, p75NTR invasion) outputs remains the central unresolved question.
  • No unifying model reconciling opposing context-dependent roles
  • Structural basis distinguishing competing partner-selection states unknown
  • Upstream signals dictating which complex PDLIM1 forms not defined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 5 GO:0060090 molecular adaptor activity 5 GO:0098772 molecular function regulator activity 4 GO:0140313 molecular sequestering activity 2
Localization
GO:0005856 cytoskeleton 5 GO:0005829 cytosol 2 GO:0005886 plasma membrane 1
Pathway
R-HSA-162582 Signal Transduction 4 R-HSA-1643685 Disease 4 R-HSA-109582 Hemostasis 2 R-HSA-168256 Immune System 2
Partners
ACTN1ACTN2ACTN4CBLBCLIK1P65/RELAPALLDYAP1
Complex memberships
E-cadherin/beta-catenin junctional complexPDLIM1/PMCA4b/alpha-actinin/actin platelet complexPDLIM1/alpha-actinin/palladin actin stress-fiber complex

Evidence

Reading pass · 24 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 CLP-36 (PDLIM1) localizes to actin stress fibers in nonmuscle cells via its PDZ domain, which associates with the spectrin-like repeats of alpha-actinin. Both nonmuscle alpha-actinin-1 and alpha-actinin-4 form complexes with CLP-36, as demonstrated by immunoprecipitation and MALDI-TOF mass spectrometry. Co-immunoprecipitation, MALDI-TOF MS, subcellular localization by fluorescence microscopy, domain deletion analysis The Journal of biological chemistry High 10753915
2000 Human CLP36 (PDLIM1) binds alpha-actinin-1 via its intervening sequence (not the PDZ or LIM domain alone), as shown by yeast two-hybrid analysis mapping to spectrin-like repeats 2 and 3 of alpha-actinin-1. CLP36 associates with actin filaments and stress fibers in activated platelets and endothelial cells and is absent from focal adhesions. The N-terminal PDZ domain plus intervening region, but not the LIM domain alone, targets CLP36 to stress fibers. Co-immunoprecipitation, pull-down assays, yeast two-hybrid, blot overlay, GFP fusion localization in endothelial cells Blood High 11110697
2000 hCLIM1 (PDLIM1) interacts with alpha-actinin-2 (skeletal muscle isoform) via its LIM domain binding to the EF-hand region of alpha-actinin-2, defining a new mode of LIM domain interaction. hCLIM1 colocalizes with alpha-actinin at Z-disks in human myocardium. Yeast two-hybrid, co-immunoprecipitation, immunofluorescence colocalization Journal of cellular biochemistry Medium 10861853
2002 CLP-36 (PDLIM1) acts as an adapter that recruits the novel kinase Clik1 to actin stress fibers via its C-terminal LIM domain. Clik1 is otherwise predominantly nuclear; association with CLP-36 relocalizes it to actin stress fibers, where it disrupts the periodic staining pattern of CLP-36. Yeast two-hybrid, subcellular colocalization, domain deletion analysis Journal of cell science Medium 11973348
2007 CLP36 (PDLIM1) is required for actin stress fiber formation and focal adhesion assembly in BeWo cells. RNAi-mediated knockdown caused loss of stress fibers and focal adhesions; rescue required both the PDZ and LIM domains, establishing that both domains are functionally essential. RNAi knockdown, rescue with domain-deletion mutants, fluorescence microscopy Biochemical and biophysical research communications Medium 17964547
2007 PDLIM1 (CLP36) is the PDZ domain-containing protein that tethers Plasma Membrane Ca2+-ATPase (PMCA) to the cytoskeleton in resting platelets via PDZ domain interactions with the C-terminal PDZ-binding motif of PMCA4b. CLP36, PMCA, alpha-actinin, and actin co-elute as a ~1,000 kDa complex by gel filtration, and CLP36 colocalizes with PMCA in both resting and activated platelets. Co-immunoprecipitation, LC-MS/MS, GST pull-down, gel filtration chromatography, immunofluorescence microscopy Thrombosis and haemostasis High 17393022
2009 CLP36 (PDLIM1) interacts with palladin via its PDZ domain binding to the C-terminus of palladin. Palladin silencing suppresses CLP36 localization to stress fibers, and overexpression of the PDZ domain alone inhibits palladin localization to stress fibers. Alpha-actinin, CLP36, and palladin form a protein complex important for actin cytoskeleton regulation. Yeast two-hybrid, co-immunoprecipitation, siRNA knockdown, overexpression of domain constructs, fluorescence microscopy The FEBS journal Medium 19366376
2009 CLP36 (PDLIM1) forms a complex with alpha-actinin in sensory (DRG) neurons, localizes to actin cytoskeleton in growth cones, and negatively regulates neurite outgrowth: RNAi knockdown of CLP36 induced lamellipodia and increased neurite length and number, whereas overexpression of the PDZ domain alone perturbed neurite outgrowth. RNAi, overexpression of deletion mutants, fluorescence microscopy, co-immunoprecipitation Journal of neurochemistry Medium 19780892
2010 CLP36 (PDLIM1) interacts with palladin in dorsal root ganglion neurons, forming a complex detectable by co-immunoprecipitation; both proteins colocalize in neurites and cell bodies of primary DRG neurons. Sciatic nerve transection upregulates both CLP36 and palladin mRNAs, with CLP36 being more prominently upregulated, suggesting a specific role in nerve regeneration. Yeast two-hybrid, co-immunoprecipitation, immunofluorescence, qRT-PCR after nerve injury Neuroscience letters Medium 20381583
2011 Alpha-actinin-4 and CLP36 (PDLIM1) form a complex in normal podocytes. siRNA depletion of alpha-actinin-4 markedly reduces CLP36 protein levels. FSGS-associated alpha-actinin-4 mutations (R310Q, Q348R) inhibit complex formation between alpha-actinin-4 and CLP36. Disruption of the alpha-actinin-4–CLP36 complex or reduction of CLP36 significantly inhibits RhoA activity and traction force generation in podocytes. Co-immunoprecipitation, siRNA knockdown, mutant overexpression, RhoA activity assay, traction force microscopy The Journal of biological chemistry High 21680739
2011 PDLIM1 exists as part of a complex with FHL1, ACTN1, GSN (gelsolin), and ACTN4, identified by tandem affinity purification from HEK-293 cells and verified by co-immunoprecipitation from mouse heart ventricles and 3D fluorescence microscopy in adult cardiomyocytes. Tandem affinity purification, LC-MS, co-immunoprecipitation, 3D fluorescence microscopy Molecular bioSystems Medium 21246116
2012 The LIM domain of PDLIM1 binds two zinc ions with femtomolar affinity (Kd avg ~2.5×10⁻¹⁴ M), and protein-protein interactions with the C-terminal extension further elevate Zn2+ affinity. Domain stability depends not only on zinc coordination but significantly on protein-protein interactions involving the C-terminus. Thermal denaturation, mass spectrometry, limited proteolysis, circular dichroism, zinc affinity measurements Journal of inorganic biochemistry High 22922308
2012 CLP36 (PDLIM1) acts as a major inhibitor of GPVI/ITAM signaling in platelets. Mice expressing truncated CLP36 lacking the LIM domain or CLP36 knockout mice displayed hyperactivation (hyperphosphorylation, enhanced Ca2+ mobilization, granule secretion, integrin activation) specifically downstream of GPVI, leading to accelerated thrombus formation in vivo. Genetic knockout and truncation mouse models, platelet functional assays (Ca2+ mobilization, integrin activation, granule secretion), in vivo thrombosis models, phosphoprotein analysis Circulation research High 22955732
2012 CLP36 and RIL (another ALP/Enigma family member) form a complex with alpha-actinin-1 and palladin on stress fibers. CLP36 depletion in F2408 fibroblasts caused non-oriented stress fiber organization; RIL compensated for this role. CLP36 and RIL have redundant roles in stress fiber formation but distinct effects on stress fiber dynamics. RNAi knockdown, time-lapse microscopy, co-immunoprecipitation, fluorescence microscopy Experimental cell research Medium 22659164
2014 CLP36 (PDLIM1) promotes breast cancer cell migration and invasion through interaction with alpha-actinin-1 and alpha-actinin-4. Mutations abolishing alpha-actinin-binding activity eliminated the ability of CLP36 to promote migration. CLP36 depletion or disruption of the CLP36–alpha-actinin complex substantially inhibited Cdc42 activation, cell polarization, and directional migration. Co-immunoprecipitation, siRNA knockdown, binding-interface mutagenesis, Cdc42 GTPase activity assay, migration/invasion assays, in vivo metastasis model Oncogene High 24662836
2015 PDLIM1 interacts with and stabilizes the E-cadherin/β-catenin complex at cell-cell junctions, thereby inhibiting β-catenin nuclear translocation and transcriptional activity and suppressing EMT in colorectal cancer cells. PDLIM1 knockdown increased nuclear β-catenin activity and promoted invasion, while overexpression attenuated EMT. Co-immunoprecipitation, siRNA knockdown, overexpression, β-catenin reporter assay, in vivo orthotopic and lung metastasis mouse models Cancer research High 26701804
2015 PDLIM1 inhibits NF-κB-mediated inflammatory signaling by sequestering the p65 subunit of NF-κB in the cytoplasm in an IκBα-independent but alpha-actinin-4-dependent manner. PDLIM1 deficiency leads to increased nuclear p65 levels and enhanced proinflammatory cytokine production in response to innate stimuli. Co-immunoprecipitation, siRNA knockdown, subcellular fractionation, nuclear p65 quantification, cytokine ELISA Scientific reports Medium 26679095
2015 PDLIM1 interacts with the neurotrophin receptor p75NTR (CD271) via its PDZ domain binding to the C-terminal SPV PDZ-binding motif of p75NTR. This interaction is regulated by S425 phosphorylation of p75NTR. The interaction also requires that p75NTR not be phosphorylated at S303 by PKA. shRNA knockdown of PDLIM1 completely ablated p75NTR-mediated glioma invasion in vitro and in vivo. Peptide-based interaction screen, co-immunoprecipitation, phosphorylation mutant analysis, shRNA knockdown, in vivo glioma invasion model Oncogene Medium 26119933
2016 PDLIM1 must be degraded via the autophagy-lysosome pathway (dependent on ATG7) to allow proper cytoskeletal reorganization during spermatid differentiation. In atg7-null mice, PDLIM1 accumulates, impairing cytoskeleton organization and leading to defects in flagella biogenesis and cytoplasm removal during spermiogenesis. Conditional knockout mouse model (atg7-null), functional screening, western blot, phenotypic analysis of sperm morphology and motility Autophagy Medium 27310465
2020 PDLIM1 competitively binds alpha-actinin-4 (ACTN4), leading to dissociation of ACTN4 from F-actin and preventing F-actin overgrowth. Loss of PDLIM1 induces excessive F-actin formation, resulting in dephosphorylation of LATS1 and activation of YAP, promoting HCC metastasis via the Hippo pathway. Asn145 (N145) of PDLIM1 is critical for its interaction with ACTN4, and N145A mutation abolishes this regulatory function. Co-immunoprecipitation, site-directed mutagenesis (N145A), F-actin binding assay, LATS1 phosphorylation assay, YAP activity measurement, in vitro invasion and in vivo metastasis models Hepatology (Baltimore, Md.) High 31509262
2020 PDLIM1 localizes to actin-rich structures generated by bacterial pathogens: it accumulates at sites of Listeria entry, in actin clouds surrounding immotile bacteria, and within actin comet/rocket tails. PDLIM1 is maintained in the actin core of membrane protrusions but absent from the membrane invagination internalizing Listeria. PDLIM1 is also a component of EPEC pedestals and its recruitment is dependent on the bacterial effector Tir. Immunofluorescence microscopy, subcellular localization during bacterial infection, Tir-deletion bacterial mutant analysis Anatomical record (Hoboken, N.J. : 2007) Medium 33022122
2022 CLP36 (PDLIM1) promotes p53-deficient sarcoma progression by inhibiting AIP-4 (ITCH E3 ligase)-dependent proteasomal degradation of YAP1, thereby increasing YAP1 protein levels. CLP36 knockout in mice markedly inhibited p53-deficiency-induced tumorigenesis. Co-immunoprecipitation, genetic KO mouse model, ubiquitination assay, western blot, tumor growth assay Theranostics Medium 35836803
2023 PDLIM1 interacts with hexokinase 2 (HK2) in gastric cancer cells, as demonstrated by co-immunoprecipitation. This interaction mediates glycolysis and cell biological behaviors under glucose deprivation through the Wnt/β-catenin signaling pathway. Co-immunoprecipitation, glucose metabolism assays (glycolytic indicators), western blot, siRNA knockdown and overexpression Cell and tissue research Low 37930472
2025 Cblb (an E3 ubiquitin ligase) binds to the PDZ and LIM domains of PDLIM1 and ubiquitinates PDLIM1 at K244, targeting it for proteasome-mediated degradation. In microglia, this Cblb-mediated degradation of PDLIM1 releases NF-κB signaling, promoting pyroptosis. Botulinum toxin A suppresses Cblb activity, thereby preventing PDLIM1 degradation and inhibiting NF-κB-mediated pyroptosis. Mass spectrometry, co-immunoprecipitation, ubiquitination assay (K244 site identification), proteasome inhibition, shRNA knockdown, lentiviral/AAV rescue experiments in vivo The journal of headache and pain Medium 40696327

Source papers

Stage 0 corpus · 49 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4. The Journal of biological chemistry 122 10753915
2016 Autophagy regulates spermatid differentiation via degradation of PDLIM1. Autophagy 119 27310465
2015 PDLIM1 Stabilizes the E-Cadherin/β-Catenin Complex to Prevent Epithelial-Mesenchymal Transition and Metastatic Potential of Colorectal Cancer Cells. Cancer research 104 26701804
2000 Human CLP36, a PDZ-domain and LIM-domain protein, binds to alpha-actinin-1 and associates with actin filaments and stress fibers in activated platelets and endothelial cells. Blood 91 11110697
2020 PDLIM1 Inhibits Tumor Metastasis Through Activating Hippo Signaling in Hepatocellular Carcinoma. Hepatology (Baltimore, Md.) 84 31509262
2002 Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein. Journal of cell science 61 11973348
2015 PDLIM1 inhibits NF-κB-mediated inflammatory signaling by sequestering the p65 subunit of NF-κB in the cytoplasm. Scientific reports 58 26679095
2000 Interaction of hCLIM1, an enigma family protein, with alpha-actinin 2. Journal of cellular biochemistry 52 10861853
2015 Glioma invasion mediated by the p75 neurotrophin receptor (p75(NTR)/CD271) requires regulated interaction with PDLIM1. Oncogene 49 26119933
2014 PDZ and LIM domain protein 1(PDLIM1)/CLP36 promotes breast cancer cell migration, invasion and metastasis through interaction with α-actinin. Oncogene 49 24662836
1999 Characterization of the human 36-kDa carboxyl terminal LIM domain protein (hCLIM1). Journal of cellular biochemistry 45 10022510
2007 The PDZ-LIM protein CLP36 is required for actin stress fiber formation and focal adhesion assembly in BeWo cells. Biochemical and biophysical research communications 38 17964547
2021 PDLIM1: Structure, function and implication in cancer. Cell stress 29 34396044
2012 Femtomolar Zn2+ affinity of LIM domain of PDLIM1 protein uncovers crucial contribution of protein-protein interactions to protein stability. Journal of inorganic biochemistry 28 22922308
2011 Identification of an FHL1 protein complex containing ACTN1, ACTN4, and PDLIM1 using affinity purifications and MS-based protein-protein interaction analysis. Molecular bioSystems 27 21246116
2007 Plasma membrane Ca(2+) -ATPase associates with CLP36, alpha-actinin and actin in human platelets. Thrombosis and haemostasis 27 17393022
2021 Serum Anti-PDLIM1 Autoantibody as Diagnostic Marker in Ovarian Cancer. Frontiers in immunology 24 34489945
2020 MicroRNA-370-3p inhibits cell proliferation and induces chronic myelogenous leukaemia cell apoptosis by suppressing PDLIM1/Wnt/β-catenin signaling. Neoplasma 23 31986893
2009 Characterization of interaction between CLP36 and palladin. The FEBS journal 22 19366376
2012 CLP36 is a negative regulator of glycoprotein VI signaling in platelets. Circulation research 21 22955732
2009 Characterization of CLP36/Elfin/PDLIM1 in the nervous system. Journal of neurochemistry 21 19780892
2011 Alpha-actinin-4 and CLP36 protein deficiencies contribute to podocyte defects in multiple human glomerulopathies. The Journal of biological chemistry 18 21680739
2010 CLP36 interacts with palladin in dorsal root ganglion neurons. Neuroscience letters 18 20381583
2012 Parent-of-origin effects of FAS and PDLIM1 in attention-deficit/hyperactivity disorder. Journal of psychiatry & neuroscience : JPN 17 21651830
2016 Autoantibodies against TYMS and PDLIM1 proteins detected as circulatory signatures in Indian breast cancer patients. Proteomics. Clinical applications 15 27068564
2021 Protective Role of microRNA-200a in Diabetic Retinopathy Through Downregulation of PDLIM1. Journal of inflammation research 13 34113148
2012 CLP36 and RIL recruit α-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts. Experimental cell research 13 22659164
2006 Expression of the actin stress fiber-associated protein CLP36 in the human placenta. Histochemistry and cell biology 11 16609848
2023 PDLIM1 interacts with HK2 to promote gastric cancer progression through enhancing the Warburg effect via Wnt/β-catenin signaling. Cell and tissue research 10 37930472
2010 Differential expression of ezrin and CLP36 in the two layers of syncytiotrophoblast in rats. Biological & pharmaceutical bulletin 10 20686238
2005 Identification of CLP36 as a tumor antigen that induces an antibody response in pancreatic cancer. Cancer research and treatment 9 19956513
2023 Oridonin Induces Apoptosis in Esophageal Squamous Cell Carcinoma by Inhibiting Cytoskeletal Protein LASP1 and PDLIM1. Molecules (Basel, Switzerland) 8 36677861
2023 Role of PDLIM1 in hepatic stellate cell activation and liver fibrosis progression. Scientific reports 8 37414929
2022 CLP36 promotes p53 deficient sarcoma progression through suppression of atrophin-1 interacting protein-4 (AIP-4)-dependent degradation of YAP1. Theranostics 8 35836803
2020 Distribution of PDLIM1 at actin-rich structures generated by invasive and adherent bacterial pathogens. Anatomical record (Hoboken, N.J. : 2007) 8 33022122
2022 miR-187/PDLIM1 Gets Involved in Gastric Cancer Progression and Cisplatin Sensitivity of Cisplatin by Mediating the Hippo-YAP Signaling Pathway. Journal of oncology 7 36164345
2020 The role of PDLIM1, a PDZ-LIM domain protein, at the ribbon synapses in the chicken retina. The Journal of comparative neurology 6 31930728
2024 Identification of PDLIM1 as a glioblastoma stem cell marker driving tumorigenesis and chemoresistance. Cell death discovery 5 39548074
2023 PDLIM1 inhibits cell migration and invasion in diabetic retinopathy via negatively regulating Wnt3a. Scientific reports 5 37037887
2024 Paeoniflorin regulates RhoA/ROCK1 and Nrf2 pathways in PDLIM1-dependent or independent manners in oxidative stressed melanocytes. Archives of dermatological research 4 38878083
2024 PDLIM1, a novel miR-3940-5p target, regulates the malignant progression of diffuse large B-cell lymphoma. Cancer biology & therapy 3 39564935
2024 Decreased PDLIM1 expression in endothelial cells contributes to the development of intracranial aneurysm. Vascular medicine (London, England) 2 38334094
2025 Botulinum toxin type A inhibits microglia pyroptosis by suppressing Cblb-mediated degradation of Pdlim1 to attenuate neuropathic pain. The journal of headache and pain 1 40696327
2026 Tumor-Derived Exosomal PDLIM1 Promotes Angiogenesis and Tumor Progression in Papillary Thyroid Carcinoma: Insights From Integrated Single-Cell Transcriptomics and Exosomal Proteomics. Cancer management and research 0 42131580
2026 Lactylation-driven PDLIM1/PDAP1 axis remodels the inflammatory landscape of acute lung injury: mechanistic insights and precision intervention. Frontiers in immunology 0 42199428
2025 PDLIM1 Inhibits Chemoresistance by Blocking DNA Damage Repair in Gastric Cancer. Recent patents on anti-cancer drug discovery 0 38779728
2025 PDLIM1 suppresses retinal neovascularization by regulating the β-catenin pathway. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 0 40116209
2025 ACTN2, regulated by PRDM9, affects the growth and inflammation of vascular smooth muscle cells by interacting with PDLIM1 in intracranial aneurysms. Frontiers in molecular neuroscience 0 40881324
2024 Regulation of lymphoma in vitro by CLP36 through the PI3K/AKT/CREB signaling pathway. PeerJ 0 39735560

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