Affinage

PCF11

Pre-mRNA cleavage complex 2 protein Pcf11 · UniProt O94913

Length
1555 aa
Mass
173.1 kDa
Annotated
2026-06-10
30 papers in source corpus 20 papers cited in narrative 20 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PCF11 is an essential subunit of cleavage factor IA (CF IA/CF I) that couples pre-mRNA 3'-end cleavage and polyadenylation to RNA polymerase II transcription termination (PMID:9032237, PMID:30819644). It was first defined as a third component of yeast CF I that physically associates with Rna14 and Rna15 and is required for in vitro cleavage and polyadenylation activity (PMID:9032237), and it forms a stable, non-catalytic ATP-bound sub-complex with Clp1 within CF IA via conserved residues that bridge CF IA to the core polyadenylation machinery (PMID:17151076, PMID:21993299). The defining mechanism resides in its N-terminal CTD-interacting domain (CID), a 140-residue module that is sufficient to dismantle elongating Pol II complexes in vitro without nucleotide hydrolysis by bridging the phosphorylated Pol II CTD to the nascent transcript (PMID:15998810, PMID:16387654); the CID engages the disordered, phospho-Ser-modified CTD through an induced-fit, trans-proline-selective interaction, and binds RNA weakly at an overlapping site, so that competitive displacement between CTD and RNA drives release of the polyadenylation machinery (PMID:15665873, PMID:16497660). A distinct C-terminal zinc-finger domain ('trillium flower' fold) and additional zinc-binding regions contribute to the cleavage and polyadenylation reaction itself (PMID:27780845, PMID:28973460). Genome-wide, PCF11 directly binds the Pol II CTD to stimulate premature cleavage/polyadenylation and transcription termination, preventing transcriptional interference at closely spaced genes and promoting intronic polyadenylation in a gene-length-dependent manner; it autoregulates its own abundance through premature termination at a conserved intronic poly(A) site (PMID:30819644, PMID:30840896). PCF11 also drives noncoding-RNA termination downstream of the Nrd1/Sen1 pathway in yeast (PMID:25877920) and is recruited to Pol II together with PNUTS by SPT6 to restrict promoter-proximal and upstream transcripts (PMID:40103229). Its termination activity is regulated post-translationally: nuclear WNK1 phosphorylates the CID to weaken CTD binding and promote transcript release and mRNA export (PMID:29196535), and the m6Am RNA modification sequesters PCF11 away from start-proximal Pol II to suppress premature termination of modified transcripts (PMID:39481383). PCF11 has additionally been co-opted as a premature termination factor that silences latent HIV-1 in concert with WDR82 (PMID:38015843).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 1997 High

    Established PCF11 as a bona fide subunit of the 3'-end processing machinery, answering whether it was merely associated or functionally required for cleavage/polyadenylation.

    Evidence Two-hybrid, co-fractionation, antibody neutralization and thermosensitive alleles in yeast CF I

    PMID:9032237

    Open questions at the time
    • Did not define which domains mediate Rna14/Rna15 contact
    • No link yet to transcription termination
  2. 2005 High

    Revealed the core molecular activity—how PCF11 terminates transcription—by showing its CID alone dismantles Pol II elongation complexes by bridging CTD to nascent RNA without ATP.

    Evidence Reconstituted in vitro dismantling and bridging assays with purified yeast Pol II and CID deletions; NMR of CTD–CID induced-fit binding

    PMID:15665873 PMID:15998810

    Open questions at the time
    • Did not establish in vivo requirement of dismantling for termination
    • Phospho-CTD recognition geometry tied to specific Ser residues not fully resolved
  3. 2006 Medium

    Clarified the release logic by showing CTD phosphopeptide and RNA compete for the same CID surface, providing a mechanism for handing off the nascent transcript.

    Evidence NMR/binding and competition assays of CID with RNA and CTD phosphopeptides

    PMID:16497660

    Open questions at the time
    • RNA binding is weak and sequence preference loosely defined
    • Single-lab biophysical study without in vivo competition demonstration
  4. 2006 High

    Extended the dismantling mechanism to metazoa and connected it to a true in vivo termination phenotype, showing the activity is conserved and acts on paused complexes.

    Evidence RNAi, ChIP and run-on at hsp70 in Drosophila plus in vitro dismantling/bridging assays

    PMID:16387654

    Open questions at the time
    • Preference for paused complexes mechanistically unexplained
    • Did not address coupling to polyadenylation in vivo
  5. 2006 High

    Defined the Clp1–Pcf11 architecture, showing Clp1 binds ATP in a SIMIBI ATPase fold without hydrolysis and contacts Pcf11 through conserved residues to form a stable CF IA sub-complex.

    Evidence X-ray crystallography of Clp1–ATP–Pcf11 and ATPase assays

    PMID:17151076

    Open questions at the time
    • Functional role of bound non-hydrolyzed ATP unresolved
    • Did not link the interface to termination in cells
  6. 2007 Medium

    Demonstrated functional conservation in human cells, linking PCF11 to both termination and degradation of the downstream cleavage product in a poly(A)-signal-dependent manner.

    Evidence siRNA knockdown in HeLa with RT-PCR/RNA analysis of readthrough and 3' product

    PMID:18086705

    Open questions at the time
    • Mechanism connecting PCF11 to the degradation machinery not defined
    • Single-lab functional study
  7. 2011 Medium

    Showed the Clp1–Pcf11 contact is functionally essential, as disrupting it (from either partner) impairs growth, 3'-end processing and termination.

    Evidence Lethal/point mutants, in vitro binding, 3'-end processing and ATP-binding assays in yeast

    PMID:21993299

    Open questions at the time
    • Separation of ATP-binding from interface effects incomplete
    • Single-lab genetic/biochemical analysis
  8. 2015 High

    Placed Pcf11 in the noncoding-RNA termination pathway, showing CID-dependent exchange with Nrd1 promotes Sen1-dependent termination and CTD Ser2 phosphorylation.

    Evidence ChIP-seq/qPCR, CID mutants, northern blots and CTD phospho-analysis in yeast

    PMID:25877920

    Open questions at the time
    • Molecular trigger for Nrd1-to-Pcf11 exchange not defined
    • Generality to mRNA terminators not addressed here
  9. 2016 High

    Assigned function to the C-terminal region by solving its novel zinc-finger fold and showing a conserved surface required for both cleavage and polyadenylation.

    Evidence NMR structure, zinc biochemistry, in vitro cleavage/polyadenylation with mutants and yeast complementation

    PMID:27780845

    Open questions at the time
    • Direct binding partner of the conserved surface unknown
    • Relationship between C-terminal and CID activities not integrated
  10. 2017 Medium

    Distinguished the contributions of multiple zinc-binding domains, showing they are dispensable for CF IA assembly and termination but contribute to the cleavage/polyadenylation reaction.

    Evidence Zinc-binding assays, NMR, CF IA assembly and 3'-end processing assays with yeast domain mutants

    PMID:28973460

    Open questions at the time
    • Precise step in cleavage/polyadenylation each domain affects unclear
    • Single-lab structural/functional study
  11. 2017 Medium

    Identified post-translational control of PCF11, showing nuclear WNK1 phosphorylates the CID to weaken CTD binding and promote transcript release and mRNA export.

    Evidence In vitro WNK1 kinase assay, co-IP, chromatin RNA fractionation and mRNA export assays in human cells

    PMID:29196535

    Open questions at the time
    • Phosphosite(s) on the CID not mapped here
    • Single-lab study; in vivo stoichiometry of phosphorylation unknown
  12. 2019 High

    Defined PCF11's genome-wide role in vertebrates, showing direct CTD-binding stimulation of premature cleavage/polyadenylation, prevention of transcriptional interference, and autoregulation via its own premature termination.

    Evidence mNET-seq, 3' mRNA-seq, chromatin RNA-seq, ChIP-seq with depletion/knockout in human cells and zebrafish

    PMID:30819644

    Open questions at the time
    • Determinants of site selection at closely spaced genes not fully resolved
    • Developmental consequences mechanistically untied to specific targets
  13. 2019 High

    Connected PCF11 dosage to intronic polyadenylation in a gene-length-dependent manner, linking its levels to expression of long migration/adhesion genes.

    Evidence siRNA across cell lines, 3' mRNA-seq, IPA-site deletion and differentiation assays

    PMID:30840896

    Open questions at the time
    • Mechanistic basis of gene-length dependence unclear
    • Direct versus indirect effects on long-gene programs not separated
  14. 2023 Medium

    Showed PCF11 can act as a dedicated premature termination factor on a viral provirus, partnering with WDR82 to silence latent HIV-1 independently of other CPA subunits.

    Evidence PCF11–WDR82 co-IP, ChIP-seq at the provirus, siRNA and reactivation assays

    PMID:38015843

    Open questions at the time
    • Structural basis of PCF11–WDR82 interaction unknown
    • Generality to host promoter-proximal termination not established
  15. 2024 High

    Established PCF11 as an m6Am reader, revealing a regulatory layer in which the modification sequesters PCF11 from start-proximal Pol II to permit full-length transcription.

    Evidence Quantitative m6Am-reader proteomics, nascent/mature RNA measurement, PCF11 knockdown with m6Am depletion and co-IP in human cells

    PMID:39481383

    Open questions at the time
    • Structural basis of PCF11 m6Am recognition not defined
    • How sequestration is spatially restricted near TSS unclear
  16. 2025 Medium

    Implicated condensate formation in PCF11 function, showing an α-helical region drives Pcf11–Spt5 condensates that stall Pol II for termination and heterochromatin formation, modulated by Spt5 phosphorylation state.

    Evidence RNAi screen, tethering assays, condensate analysis and PP1/PNUTS epistasis in Drosophila germ cells

    PMID:40015272

    Open questions at the time
    • No in vitro reconstitution of condensates
    • Relationship between condensate and CID-dependent dismantling mechanisms unresolved
  17. 2025 Medium

    Positioned PCF11 within SPT6-directed termination, showing SPT6 recruits PNUTS and PCF11 to Pol II with distinct and overlapping roles in restricting upstream/promoter-proximal transcripts.

    Evidence siRNA of SPT6/IWS1/PCF11/PNUTS, co-IP, ChIP and RNA-seq readthrough analysis in human cells

    PMID:40103229

    Open questions at the time
    • Direct versus SPT6-bridged recruitment of PCF11 not distinguished
    • Why PCF11 is dispensable for normal PROMPT restriction unexplained
  18. 2025 Low

    Linked PCF11 to influenza virus replication via interaction with viral nucleoprotein and suppression of IFN-β signaling, expanding its proposed host-factor roles.

    Evidence Co-IP with viral NP, knockdown replication assays, polymerase and IFN-β reporter assays

    PMID:40252262

    Open questions at the time
    • No structural or reconstitution evidence for the NP interaction
    • Single-lab study; mechanism of IFN-β regulation undefined

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the CID-dependent dismantling activity, the C-terminal cleavage/polyadenylation function, condensate formation, and modification-based sequestration are integrated into a single regulated decision to terminate at a given site remains unresolved.
  • No unified structural model coupling CID and zinc-finger activities
  • Determinants of premature versus normal termination site choice undefined
  • In vivo phosphorylation/modification thresholds governing PCF11 activity unmapped

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 2 GO:0060090 molecular adaptor activity 2 GO:0140098 catalytic activity, acting on RNA 2 GO:0140110 transcription regulator activity 2
Localization
GO:0005634 nucleus 2 GO:0005694 chromosome 2
Pathway
R-HSA-74160 Gene expression (Transcription) 3 R-HSA-8953854 Metabolism of RNA 3
Partners
CLP1PNUTSRNA14RNA15SPT5SPT6WDR82WNK1
Complex memberships
Cleavage Factor IA (CF IA / CF I)Clp1–Pcf11 sub-complexPCF11–WDR82 premature termination complex

Evidence

Reading pass · 20 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 PCF11 encodes a third protein component of yeast Cleavage Factor I (CF I), the complex required for pre-mRNA 3'-end cleavage and polyadenylation. PCF11 physically associates with Rna14 and Rna15 (co-fractionation, two-hybrid), and its inactivation by antibody neutralization or thermosensitive mutation abolishes cleavage and polyadenylation activity in vitro. Two-hybrid screen, co-fractionation by anion-exchange chromatography, antibody neutralization of in vitro cleavage/polyadenylation, thermosensitive allele analysis Molecular and cellular biology High 9032237
2005 A 140-amino-acid CTD-interacting domain (CID) of yeast Pcf11 is sufficient to dismantle RNA Pol II elongation complexes in vitro. This activity requires both the Pol II CTD and the Pcf11 CID, and proceeds without nucleotide hydrolysis. Pcf11 bridges the CTD to the nascent transcript, causing dissociation of both Pol II and the nascent RNA from the DNA template. In vitro elongation complex dismantling assay using purified yeast RNA Pol II; domain-deletion analysis of CID requirement; bridging assay Genes & development High 15998810
2005 The phosphorylated Pol II CTD exists as a disordered ensemble in solution but adopts a structured conformation upon binding to the Pcf11 CID (induced fit). Only the all-trans proline form of the CTD is selected for binding, and binding is phospho-dependent. NMR spectroscopy (structural analysis of CTD–Pcf11 CID interaction), cis-trans isomer discrimination assay Nature structural & molecular biology High 15665873
2006 The Pcf11 CID binds RNA with weak but sequence-preferential activity. CTD-derived phosphopeptides and RNA compete for the same binding site on the Pcf11 CID, suggesting that competitive displacement of RNA by the CTD (or vice versa) is mechanistically important for releasing polyadenylation factors from Pol II. NMR/binding assays comparing RNA–CID and phosphopeptide–CID interactions; competition experiments RNA (New York, N.Y.) Medium 16497660
2006 Drosophila Pcf11 (dPcf11) is a direct termination factor in vivo: it is concentrated at the 3' end of the hsp70 gene, and RNAi depletion of dPcf11 causes Pol II to read through normal termination regions. Biochemically, dPcf11 dismantles elongation complexes via a CTD-dependent, nucleotide-independent mechanism by bridging the CTD to nascent RNA. dPcf11 preferentially dismantles paused elongation complexes. RNAi knockdown with ChIP and run-on assays in Drosophila cells; in vitro elongation complex dismantling; RNA–CTD bridging assay Molecular cell High 16387654
2006 Crystal structure of a ternary Clp1–ATP–Pcf11 complex reveals that Clp1 contains three domains with the ATP-binding site in the central domain (SIMIBI-class ATPase fold), but ATP is not hydrolysed. Three highly conserved Pcf11 residues in the central domain mediate Clp1–Pcf11 protein–protein contacts, forming a stable sub-complex within CF IA. X-ray crystallography of Clp1–ATP–Pcf11 ternary complex; ATPase activity assay (hydrolysis not detected) Nucleic acids research High 17151076
2007 Human PCF11 (hPcf11) is required for efficient transcription termination and for degradation of the 3' RNA cleavage product generated downstream of the poly(A) site. Both functions require an intact poly(A) signal. siRNA knockdown in HeLa cells; RT-PCR and RNA analysis measuring termination readthrough and 3' cleavage-product accumulation Nucleic acids research Medium 18086705
2011 Clp1 bridges CF IA and CPF through its N-terminal and central domains. Mutations in the Clp1 ATP-binding site that displace ATP do not affect ATP binding per se but disrupt the Clp1–Pcf11 interaction, and a reciprocal Pcf11 mutation disrupting the Clp1 contact causes defects in growth, 3'-end processing, and transcription termination. Genetic analysis (lethal mutations), in vitro binding assays, 3'-end processing assays, ATP-binding assays with mutants Nucleic acids research Medium 21993299
2015 In S. cerevisiae, Pcf11 (via its CID) is required for NRD-dependent termination of noncoding RNAs. Pcf11 localizes downstream of Nrd1 on NRD terminators, its recruitment depends on Nrd1, and mutation of the Pcf11 CID causes Nrd1 retention on chromatin, delayed ncRNA degradation, restricted Pol II CTD Ser2 phosphorylation, and reduced Sen1–Pol II interaction. Exchange of Nrd1 for Pcf11 on chromatin facilitates Pol II pausing and CTD Ser2 phosphorylation to promote Sen1-dependent termination. ChIP-seq, ChIP-qPCR, CID mutation analysis, mRNA/ncRNA northern blotting, Pol II CTD phosphorylation analysis in yeast Genes & development High 25877920
2016 The C-terminal domain of Pcf11 adopts a novel zinc-finger fold ('trillium flower'). A conserved surface on this domain is critical for both cleavage and polyadenylation activities, as shown by structural, biochemical, and genetic analyses. NMR structure determination of C-terminal domain; zinc coordination biochemistry; in vitro cleavage/polyadenylation assays with surface mutants; genetic complementation in yeast RNA (New York, N.Y.) High 27780845
2017 Nuclear WNK1 kinase phosphorylates the PCF11 CID, weakening its interaction with Pol II CTD. This phosphorylation promotes transcript release from chromatin-associated Pol II, facilitating mRNA export to the cytoplasm. In vitro kinase assay (WNK1 phosphorylating PCF11 CID); co-immunoprecipitation; chromatin RNA fractionation; mRNA export assays upon WNK1 inhibition or depletion Genes & development Medium 29196535
2017 Yeast Pcf11 contains two separate zinc-binding domains flanking the Clp1-interaction region. These zinc-binding domains are dispensable for CF IA assembly and Pol II termination but contribute to different extents to the pre-mRNA 3'-end cleavage and polyadenylation mechanism. Zinc-binding assays; NMR structural analysis of the second zinc-binding domain; CF IA assembly assays; 3'-end processing assays with domain mutants Nucleic acids research Medium 28973460
2019 PCF11 directly binds the Pol II CTD and stimulates premature cleavage/polyadenylation and transcription termination genome-wide in vertebrates. PCF11 preferentially acts at closely spaced genes to prevent transcriptional interference. PCF11 levels are controlled by an auto-regulatory mechanism whereby PCF11 promotes premature termination of its own transcript. mNET-seq, 3' mRNA-seq, chromatin RNA-seq, ChIP-seq in human cells and zebrafish; PCF11 depletion/conditional knockout; zebrafish development assays Molecular cell High 30819644
2019 PCF11 promotes intronic polyadenylation (IPA) in a gene-length-dependent manner: downregulation of PCF11 suppresses IPA in large introns, leading to upregulation of long genes involved in cell morphology, adhesion, and migration, while short genes are downregulated. PCF11 is itself autoregulated through a conserved IPA site. siRNA knockdown in multiple cell lines; 3' mRNA-seq; IPA analysis; cell differentiation assays; IPA-site deletion experiments Cell reports High 30840896
2023 PCF11 and WDR82 form a premature transcription termination complex that silences HIV-1 expression in latently infected cells. PCF11 represses HIV-1 independently of other CPA complex subunits or the 5' LTR poly(A) signal. PCF11 and WDR82 interact with each other, are co-recruited to the promoter-proximal region of the HIV-1 provirus in an interdependent manner, and act on the same pathway. Co-immunoprecipitation of PCF11–WDR82; ChIP-seq at HIV-1 provirus; siRNA knockdown of PCF11 and WDR82; HIV-1 reactivation assays Proceedings of the National Academy of Sciences of the United States of America Medium 38015843
2024 PCF11 is identified as a direct m6Am-specific reader protein in human cells. m6Am functions by sequestering PCF11 away from proximal Pol II, suppressing PCF11-mediated dissociation of Pol II near transcription start sites and thereby promoting full-length transcription of m6Am-modified RNAs. During ATRA-induced neuroblastoma differentiation, reduced PCF11 levels amplify m6Am's anti-termination effect. Quantitative proteomics (m6Am-reader pulldown); direct measurement of mature vs. nascent RNA levels; PCF11 knockdown combined with m6Am depletion; co-immunoprecipitation Molecular cell High 39481383
2025 Pcf11 and Spt5 form condensates that stall Pol II to facilitate transcription termination and piRNA-guided heterochromatin formation in Drosophila germ cells. An α-helical region of Pcf11 drives condensate formation; Pcf11 preferentially forms condensates with unphosphorylated Spt5, a state promoted by the PP1/PNUTS phosphatase. Enforced Pcf11 tethering causes co-transcriptional repression dependent on this condensate-forming region. RNAi screen in Drosophila germ line; enforced tethering assays; condensate formation analysis (phase separation); Spt5 phosphorylation state analysis; genetic epistasis with PP1/PNUTS Molecular cell Medium 40015272
2025 SPT6 facilitates transcription termination partly by recruiting PNUTS and PCF11 to Pol II. PCF11 is required for accumulation of promoter upstream transcripts (PROMPTs) in the absence of SPT6, while SPT6 and PNUTS (but not PCF11) jointly restrict PROMPTs under normal conditions, indicating distinct and overlapping termination functions. siRNA knockdown of SPT6, IWS1, PCF11, and PNUTS; co-immunoprecipitation; ChIP; RNA-seq; readthrough transcription analysis Nucleic acids research Medium 40103229
2025 PCF11 promotes proliferation of swine influenza virus (SIV) by interacting with viral nucleoprotein (NP), promoting vRNP assembly and polymerase activity, and negatively regulating the IFN-β signaling pathway. Co-immunoprecipitation of PCF11 with viral NP; PCF11 knockdown with viral replication assays; polymerase activity reporter assays; IFN-β pathway reporter assays Microbiological research Low 40252262
2025 In Drosophila male germ cells, PCF11 and its partner Cbc change subnuclear localization during spermatocyte differentiation, transitioning from homogeneous nuclear distribution in spermatogonia and early spermatocytes to concentrated peri-nucleolar localization in later spermatocytes, concurrent with developmentally regulated 3' UTR shortening. Immunofluorescence microscopy; live imaging of subnuclear localization across germ cell stages microPublication biology Low 40896257

Source papers

Stage 0 corpus · 30 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2019 Selective Roles of Vertebrate PCF11 in Premature and Full-Length Transcript Termination. Molecular cell 118 30819644
2005 CTD-dependent dismantling of the RNA polymerase II elongation complex by the pre-mRNA 3'-end processing factor, Pcf11. Genes & development 112 15998810
2005 Key features of the interaction between Pcf11 CID and RNA polymerase II CTD. Nature structural & molecular biology 103 15665873
2006 Pcf11 is a termination factor in Drosophila that dismantles the elongation complex by bridging the CTD of RNA polymerase II to the nascent transcript. Molecular cell 89 16387654
1997 PCF11 encodes a third protein component of yeast cleavage and polyadenylation factor I. Molecular and cellular biology 88 9032237
2019 Regulation of Intronic Polyadenylation by PCF11 Impacts mRNA Expression of Long Genes. Cell reports 86 30840896
2018 Transcriptome 3'end organization by PCF11 links alternative polyadenylation to formation and neuronal differentiation of neuroblastoma. Nature communications 86 30552333
2015 Pcf11 orchestrates transcription termination pathways in yeast. Genes & development 67 25877920
2006 Structure of a nucleotide-bound Clp1-Pcf11 polyadenylation factor. Nucleic acids research 62 17151076
2007 Human Pcf11 enhances degradation of RNA polymerase II-associated nascent RNA and transcriptional termination. Nucleic acids research 50 18086705
2011 The interaction of Pcf11 and Clp1 is needed for mRNA 3'-end formation and is modulated by amino acids in the ATP-binding site. Nucleic acids research 29 21993299
2006 RNA polymerase II CTD phosphopeptides compete with RNA for the interaction with Pcf11. RNA (New York, N.Y.) 27 16497660
2017 WNK1 kinase and the termination factor PCF11 connect nuclear mRNA export with transcription. Genes & development 18 29196535
2016 The C terminus of Pcf11 forms a novel zinc-finger structure that plays an essential role in mRNA 3'-end processing. RNA (New York, N.Y.) 17 27780845
2012 Identification of Inverse Regulator-a (Inr-a) as Synonymous with Pre-mRNA Cleavage Complex II Protein (Pcf11) in Drosophila. G3 (Bethesda, Md.) 16 22690379
2024 m6Am sequesters PCF11 to suppress premature termination and drive neuroblastoma differentiation. Molecular cell 14 39481383
2017 Distinct roles of Pcf11 zinc-binding domains in pre-mRNA 3'-end processing. Nucleic acids research 13 28973460
1992 The PCF1-1 mutation increases the activity of the transcription factor (TF) IIIB fraction from Saccharomyces cerevisiae. Nucleic acids research 12 1641338
2025 Pcf11/Spt5 condensates stall RNA polymerase II to facilitate termination and piRNA-guided heterochromatin formation. Molecular cell 8 40015272
2023 Premature transcription termination complex proteins PCF11 and WDR82 silence HIV-1 expression in latently infected cells. Proceedings of the National Academy of Sciences of the United States of America 8 38015843
2009 Involvement of Pta1, Pcf11 and a KlCYC1 AU-rich element in alternative RNA 3'-end processing selection in yeast. FEBS letters 7 19646984
2015 Chemical shift assignments of a new folded domain from yeast Pcf11. Biomolecular NMR assignments 5 26133941
2022 PCF11, a Novel CD44-Downstream Transcriptional Target, Linking Its 3'-End Polyadenylation Function to Tumor Cell Metastasis. Frontiers in oncology 4 35756640
2025 Overlapping and distinct functions of SPT6, PNUTS, and PCF11 in regulating transcription termination. Nucleic acids research 3 40103229
2024 Mutations in yeast Pcf11, a conserved protein essential for mRNA 3' end processing and transcription termination, elicit the Environmental Stress Response. Genetics 2 37967370
2024 Cap-specific m6Am modification: A transcriptional anti-terminator by sequestering PCF11 with implications for neuroblastoma therapy. Molecular cell 2 39515291
2024 Computational insight into crucial interaction between Pcf11 and Ydh1 for pre-mRNA 3'-end processing. Journal of biomolecular structure & dynamics 1 39660558
2025 Pre-mRNA cleavage complex II protein Pcf11 facilitates swine influenza virus replication by interacting with viral NP and promoting polymerase activity. Microbiological research 0 40252262
2025 The CFII components PCF11 and Cbc change subnuclear localization as cells differentiate in the male germ line adult stem cell lineage. bioRxiv : the preprint server for biology 0 40777464
2025 The CFII components PCF11 and Cbc change subnuclear localization as cells differentiate in an adult stem cell lineage. microPublication biology 0 40896257

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