Affinage

CLP1

Polyribonucleotide 5'-hydroxyl-kinase Clp1 · UniProt Q92989

Length
425 aa
Mass
47.6 kDa
Annotated
2026-06-09
61 papers in source corpus 16 papers cited in narrative 16 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CLP1 is an RNA-specific 5'-OH polynucleotide kinase that serves as a shared catalytic and structural component of two RNA processing machineries: the tRNA splicing endonuclease (TSEN) complex and the mRNA 3'-end cleavage/polyadenylation apparatus (PMID:17495927, PMID:24766809, PMID:21993299). In tRNA splicing, CLP1 phosphorylates the 5' end of the 3' tRNA exon to license exon ligation, and it can also phosphorylate synthetic siRNAs to enable their incorporation into RISC (PMID:17495927); its kinase activity is sufficient to substitute for the kinase module of yeast/plant tRNA ligases in vivo, while yeast Clp1 lacks detectable kinase activity (PMID:18648070). Structural work defines a three-domain architecture (N-terminal β-sandwich, central SIMIBI-like ATP-binding domain, C-terminal α/β fold) in which an RNA-binding 'clasp' motif confers substrate specificity and a Walker A lysine governs a catalytic conformational switch (PMID:24813946, PMID:17151076). CLP1 associates with the TSEN complex to support pre-tRNA cleavage (PMID:24766809, PMID:24766810), and within CF IA it bridges to the cleavage and polyadenylation factor through its N-terminal and ATP-binding domains, where ATP binding is required not for hydrolysis but for the Clp1–Pcf11 interaction and CF IA assembly that drives poly(A) site selection and RNA Pol II transcription termination (PMID:17151076, PMID:21993299, PMID:22216186, PMID:21993300). Loss of CLP1 kinase activity uncouples these functions pathologically: aberrant tRNA-derived fragments (from tyrosine and isoleucine pre-tRNAs) accumulate and sensitize neurons to oxidative-stress-induced p53-dependent death, with p53 deletion rescuing motor neuron loss in kinase-dead mice (PMID:23474986, PMID:34273619). The human CLP1 p.R140H mutation destabilizes CLP1–TSEN association, impairs pre-tRNA cleavage, and shifts poly(A) site usage, causing the neurodegenerative disease pontocerebellar hypoplasia type 10 (PMID:24766809, PMID:24766810, PMID:34548404).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 2007 High

    Established CLP1's core biochemical identity by showing it is the long-sought 5'-OH RNA kinase that phosphorylates the 3' tRNA exon to permit ligation, also acting on siRNAs for RISC loading.

    Evidence Chromatographic purification of kinase activity from HeLa cells with in vitro RNA kinase and RISC assembly assays

    PMID:17495927

    Open questions at the time
    • Did not resolve the structural basis of RNA specificity
    • Did not establish the physiological consequence of kinase loss in an organism
  2. 2008 High

    Demonstrated that human CLP1 kinase activity is functionally a tRNA splicing enzyme and that this activity is species-divergent, since yeast Clp1 has none.

    Evidence In vivo complementation of kinase-defective yeast tRNA ligase mutants by hCLP1, recombinant yClp1 kinase assay, active-site mutagenesis

    PMID:18648070

    Open questions at the time
    • Did not explain why yeast retains a kinase-dead Clp1
    • Did not map the kinase to a complex in human cells
  3. 2006 High

    Defined CLP1's three-domain architecture and its non-hydrolytic, SIMIBI-like nucleotide-binding site, and identified the Pcf11 binding interface, revealing its scaffolding role in 3'-end processing.

    Evidence X-ray crystallography of yeast Clp1 in ternary complex with ATP and the Pcf11 Clp1-binding region

    PMID:17151076

    Open questions at the time
    • Did not address human CLP1 kinase mechanism
    • Structure was of the kinase-inactive yeast ortholog
  4. 2011 High

    Resolved CLP1's structural role in mRNA 3'-end processing by showing it bridges CF IA to CPF, requires ATP binding (not hydrolysis) for the Pcf11 interaction, and is essential for CF IA assembly and Pol II transcription termination.

    Evidence Yeast two-hybrid, co-IP, in vitro CF IA reconstitution, P-loop mutagenesis, ChIP, and depletion phenotypes across three companion studies

    PMID:21993299 PMID:21993300 PMID:22216186

    Open questions at the time
    • Performed in yeast where Clp1 lacks kinase activity, leaving the human 3'-processing role inferred
    • Did not connect 3'-processing function to disease
  5. 2013 High

    Linked CLP1 kinase loss to a disease-relevant mechanism by showing kinase-dead mice accumulate aberrant tyrosine pre-tRNA fragments that drive oxidative-stress-induced p53-dependent motor neuron death.

    Evidence Kinase-dead knock-in mouse, small RNA sequencing, p53 knockout epistasis rescue, neuromuscular analysis

    PMID:23474986

    Open questions at the time
    • Did not define how tRNA fragments activate p53
    • Did not establish the human genetic link
  6. 2014 High

    Identified CLP1 p.R140H as a human disease mutation causing pontocerebellar hypoplasia by disrupting TSEN complex association and pre-tRNA cleavage, with neuronal and oxidative-stress phenotypes recapitulated across mice, zebrafish, and patient cells.

    Evidence Patient genome sequencing, TSEN co-IP/stability assays, pre-tRNA cleavage assays, zebrafish null rescue, patient-derived induced neurons, kinase-dead mouse histology

    PMID:24766809 PMID:24766810

    Open questions at the time
    • Did not fully separate the TSEN/tRNA defect from the 3'-processing defect in disease
    • Mechanism of progenitor cell death not molecularly resolved
  7. 2014 High

    Established the structural mechanism of CLP1 RNA specificity and catalytic regulation, identifying the 'clasp' RNA-binding motif and a Walker A lysine conformational switch.

    Evidence X-ray crystallography of C. elegans Clp1 in nucleotide- and RNA-bound states with biochemical kinase assays and mutagenesis

    PMID:24813946

    Open questions at the time
    • Structures were of the C. elegans ortholog
    • Did not connect the switch to human disease mutations
  8. 2020 High

    Refined the disease model by showing CLP1 R140H dysregulates intron-containing tRNA gene products and shifts poly(A) site usage, implicating both tRNA and mRNA 3'-processing defects in PCH10.

    Evidence Two independent knock-in mouse models with tRNA gene product profiling and poly(A) site sequencing in spinal cord

    PMID:34548404

    Open questions at the time
    • Did not determine relative contribution of tRNA versus poly(A) defects to neurodegeneration
    • Causality of poly(A) shifts for phenotype not established
  9. 2020 Medium

    Clarified CLP1 as the dominant cellular siRNA 5'-kinase by showing the related kinase NOL9 cannot substitute for it.

    Evidence siRNA efficiency assays in Clp1 kinase-dead cells, recombinant NOL9 kinase assays, and NOL9 overexpression rescue attempts

    PMID:32081435

    Open questions at the time
    • Single-lab study
    • Did not address whether other kinases compensate in vivo
  10. 2020 Medium

    Extended CLP1's mRNA 3'-processing role to immune tolerance by showing it promotes 3'-UTR shortening that stabilizes Aire-sensitive transcripts in thymic medullary epithelial cells.

    Evidence RNAi screen, lentigenic mouse model, 3'-end sequencing, transcript stability assays

    PMID:32338592

    Open questions at the time
    • Single-lab study
    • Mechanistic link between CLP1 and specific poly(A) site choice not resolved
  11. 2021 Medium

    Broadened the spectrum of pathogenic RNA species in PCH10 by showing R140H mice accumulate both isoleucine pre-tRNA intron fragments and tyrosine-derived 5' tRNA fragments.

    Evidence R140H knock-in mouse with RNA fragment profiling and motor neuron loss quantification

    PMID:34273619

    Open questions at the time
    • Did not determine whether the two fragment types act cooperatively or independently
    • Single-lab study
  12. 2021 Medium

    Revealed a conserved developmental role for CLP1 in promoting meiosis entry through interactions with TSEN54 and the VCP ortholog, with cross-species functional rescue.

    Evidence Drosophila testis genetics, co-IP, Tsen54-Cbc domain mapping, localization, and mammalian CLP1 rescue of fly fertility

    PMID:33864361

    Open questions at the time
    • Performed in Drosophila
    • Mechanism connecting tRNA processing to meiosis entry unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • How CLP1 partitions its kinase activity and scaffolding between TSEN-mediated tRNA splicing and CF IA-mediated mRNA 3'-end processing, and how the relative loss of each function determines tissue-specific neurodegeneration, remains unresolved.
  • No structure of human CLP1 within either intact human complex
  • Quantitative contribution of tRNA-fragment toxicity versus poly(A)/3'-processing defects to PCH10 undefined
  • Molecular pathway connecting tRNA fragments to p53 activation not identified

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140098 catalytic activity, acting on RNA 3 GO:0140657 ATP-dependent activity 3 GO:0003723 RNA binding 2 GO:0016740 transferase activity 2 GO:0060090 molecular adaptor activity 2
Localization
GO:0005634 nucleus 1
Pathway
R-HSA-1643685 Disease 3 R-HSA-74160 Gene expression (Transcription) 3 R-HSA-8953854 Metabolism of RNA 3 R-HSA-5357801 Programmed Cell Death 1
Partners
NOL9PCF11TSEN54VCPYSH1
Complex memberships
CF IATSEN complex

Evidence

Reading pass · 16 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2007 hClp1 (human CLP1) is an RNA-specific 5'-OH polynucleotide kinase that phosphorylates the 5' end of the 3' tRNA exon during human tRNA splicing, enabling subsequent ligation of both exon halves. It also phosphorylates synthetic siRNAs at the 5' end, licensing them for incorporation into RISC and subsequent target RNA cleavage. Chromatographic purification of kinase activity from HeLa cells, in vitro RNA kinase assay, siRNA phosphorylation monitoring, RISC assembly/cleavage assay Nature High 17495927
2008 Human CLP1 kinase activity can functionally substitute for the 5'-OH RNA kinase module of yeast/plant tRNA ligases in vivo, demonstrating its role as a tRNA splicing enzyme. Mutations in the kinase active site abolish this tRNA splicing activity. Yeast Clp1, unlike human CLP1, has no detectable RNA kinase activity in vitro. Complementation of conditional and lethal kinase-defective tRNA ligase mutations in budding yeast by hCLP1 expression; in vitro RNA kinase assay with purified recombinant yClp1; kinase active-site mutagenesis RNA High 18648070
2013 Loss of CLP1 kinase activity in mice (kinase-dead Clp1 K/K) causes progressive spinal motor neuron loss. Mechanistically, loss of CLP1 activity results in accumulation of small RNA fragments derived from aberrant processing of tyrosine pre-tRNA. These tRNA fragments sensitize cells to oxidative-stress-induced p53 activation and p53-dependent cell death. Genetic inactivation of p53 rescues motor neuron loss, muscle denervation, and respiratory failure in Clp1 K/K mice. Kinase-dead knock-in mouse model; small RNA sequencing; p53 genetic knockout epistasis; neuromuscular junction analysis; motor function assays Nature High 23474986
2014 A human CLP1 missense mutation (p.R140H) causes loss of CLP1 interaction with the tRNA splicing endonuclease (TSEN) complex, largely reduced pre-tRNA cleavage activity, and accumulation of linear tRNA introns. CLP1 kinase-dead mice also display microcephaly and reduced cortical brain volume due to enhanced cell death of neuronal progenitors. Patient genome sequencing; co-immunoprecipitation of CLP1-TSEN complex; pre-tRNA cleavage assay; kinase-dead mouse histology and neuronal progenitor analysis Cell High 24766809
2014 The CLP1 founder mutation (R140H) causes defective CLP1 kinase activity and destabilization of the TSEN complex, resulting in impaired pre-tRNA cleavage. Patient-derived induced neurons display depletion of mature tRNAs and accumulation of unspliced pre-tRNAs. Transfection of partially processed tRNA fragments into patient cells exacerbates oxidative stress-induced reduction in cell survival. CLP1 kinase assay; TSEN complex co-immunoprecipitation/stability assay; zebrafish germline clp1 null rescue with wild-type vs. mutant human CLP1; tRNA northern blot from patient-derived induced neurons; tRNA fragment transfection + oxidative stress survival assay Cell High 24766810
2014 Crystal structures of C. elegans Clp1 (ceClp1) in nucleotide- and RNA-bound states define the RNA specificity mechanism: an RNA binding motif termed 'clasp' confers RNA substrate specificity, and a conformational switch involving the essential Walker A lysine (Lys127) regulates enzymatic activity. This switch is proposed as a broadly conserved mechanistic feature of P-loop proteins. X-ray crystallography; biochemical kinase assays; active-site mutagenesis Molecular Cell High 24813946
2006 Crystal structure of yeast Clp1 in ternary complex with ATP and the Clp1-binding region of Pcf11 reveals three domains (N-terminal beta sandwich, central ATP-binding, C-terminal alpha/beta-fold). The nucleotide-binding site resembles SIMIBI-class ATPases but does not hydrolyze ATP. Three highly conserved Pcf11 residues mediate most protein-protein contacts at the central domain. X-ray crystallography (ternary complex structure) Nucleic Acids Research High 17151076
2011 Yeast Clp1 interacts with CPF (Cleavage and Polyadenylation Factor) through its N-terminal and central domains, providing cross-factor connections in the mRNA 3'-processing complex. Mutations in the conserved ATP-binding site that prevent ATP binding disrupt the Clp1-Pcf11 interaction (rather than ATP binding per se). Mutations in Pcf11 that disrupt Clp1 contact cause defects in 3'-end processing and transcription termination. Yeast two-hybrid; co-immunoprecipitation; in vitro reconstitution of mutant CFIA; coupled in vitro transcription/3'-end processing assays; growth assays Nucleic Acids Research High 21993299
2011 Yeast Clp1 is required to assemble recombinant CF IA; depletion of Clp1 in yeast causes defective mRNA 3'-end formation and RNA Pol II transcription termination. The P-loop (ATP-binding) motif of Clp1 plays a structural role in CF IA organization, with ATP binding contributing to CF IA assembly and cross-factor interactions with CPF component Ysh1. Yeast Clp1 depletion; in vitro transcription/3'-end processing complementation assay; P-loop mutagenesis; recombinant CF IA reconstitution; interaction assays with CPF subunits PLoS ONE High 22216186
2011 Yeast Clp1 depletion abolishes RNA Pol II transcription termination. Clp1 is essential for CF IA assembly and transmits conformational changes to RNA Pol II through Pcf11 to couple transcription termination with 3'-end processing. Clp1 depletion in yeast; ChIP analysis of Rna15 and Pcf11 at gene 3'-ends; ATP-binding domain and Pcf11-binding region double mutant analysis; 3'-end processing assays Nucleic Acids Research High 21993300
2020 CLP1 R140H mutation in mouse models of PCH10 dysregulates products of intron-containing tRNA genes (pre-tRNAs, introns, and certain tRNA fragments up-regulated; other fragments down-regulated) without affecting mature tRNA levels. Additionally, CLP1 mutation shifts poly(A) site usage from proximal to distal sites in spinal cord, particularly in short and closely spaced genes, consistent with impaired mRNA 3' processing. Knock-in mouse models (homozygous R140H and compound heterozygous); tRNA gene product profiling; poly(A) site usage sequencing; gene expression analysis in spinal cord Proceedings of the National Academy of Sciences of the United States of America High 34548404
2020 CLP1 is the main RNA kinase phosphorylating the 5' end of siRNAs in mouse cells; NOL9 (a related RNA kinase) shows no apparent RNA kinase activity in mouse cells or with recombinant protein, and NOL9 overexpression does not rescue reduced siRNA efficiency in CLP1 kinase-dead cells. siRNA efficiency assay in Clp1 K/K cells; recombinant murine NOL9 in vitro RNA kinase assay; NOL9 overexpression rescue experiment Biochemical and Biophysical Research Communications Medium 32081435
2021 CLP1 R140H mutation causes intracellular accumulation of both isoleucine pre-tRNA intron fragments (Ile-introns) and 5' tRNA fragments derived from tyrosine pre-tRNAs in knock-in mice, suggesting two types of aberrant RNA fragments may cooperatively or independently contribute to PCH10 pathogenesis. CLP1 R140H knock-in mouse generation; RNA fragment analysis by northern blot/sequencing; motor neuron loss quantification Biochemical and Biophysical Research Communications Medium 34273619
2020 CLP1 promotes 3'-UTR shortening associated with higher transcript stability and expression of Aire-sensitive genes in thymic medullary epithelial cells, representing a post-transcriptional level of control via the 3'-end processing complex. RNAi screen; lentigenic mouse model; 3'-end sequencing; transcript stability assays eLife Medium 32338592
2021 In Drosophila, nuclear Cbc (the CLP1 ortholog) is required to promote meiosis entry in the testis. Cbc physically and/or genetically interacts with Tsen54 (the C-terminal half of Tsen54 is necessary and sufficient for binding) and TER94 (VCP ortholog) in this process. Mammalian CLP1 can rescue Drosophila fertility defects, demonstrating functional conservation. Genetic manipulation in Drosophila testis; co-immunoprecipitation; domain mapping of Tsen54-Cbc interaction; subcellular localization assay; mammalian CLP1 rescue of Drosophila fertility Human Molecular Genetics Medium 33864361
1996 The HEAB gene (human CLP1 alias) encodes a 425-amino acid protein containing an ATP/GTP-binding motif with homology to the ATP-binding transporter superfamily or GTP-binding proteins. It is expressed ubiquitously, with highest expression in testis and skeletal muscle. cDNA cloning; Northern blot; sequence analysis Blood Low 8896421

Source papers

Stage 0 corpus · 61 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2014 CLP1 founder mutation links tRNA splicing and maturation to cerebellar development and neurodegeneration. Cell 227 24766810
2013 CLP1 links tRNA metabolism to progressive motor-neuron loss. Nature 211 23474986
2014 Human CLP1 mutations alter tRNA biogenesis, affecting both peripheral and central nervous system function. Cell 181 24766809
2007 The human RNA kinase hClp1 is active on 3' transfer RNA exons and short interfering RNAs. Nature 180 17495927
2001 The membrane-type collectin CL-P1 is a scavenger receptor on vascular endothelial cells. The Journal of biological chemistry 173 11564734
2008 The Clp1/Cdc14 phosphatase contributes to the robustness of cytokinesis by association with anillin-related Mid1. The Journal of cell biology 83 18378776
2006 The Clp1 protein is required for clamp formation and pathogenic development of Ustilago maydis. The Plant cell 79 16920779
2016 The collectins CL-L1, CL-K1 and CL-P1, and their roles in complement and innate immunity. Immunobiology 71 27377710
2001 The clp1 gene of the mushroom Coprinus cinereus is essential for A-regulated sexual development. Genetics 71 11139497
2008 The SIN kinase Sid2 regulates cytoplasmic retention of the S. pombe Cdc14-like phosphatase Clp1. Current biology : CB 67 18951025
2006 Structure of a nucleotide-bound Clp1-Pcf11 polyadenylation factor. Nucleic acids research 62 17151076
2004 Ablation of the CLP-1 gene leads to down-regulation of the HAND1 gene and abnormality of the left ventricle of the heart and fetal death. Mechanisms of development 55 15172687
2008 Human RNA 5'-kinase (hClp1) can function as a tRNA splicing enzyme in vivo. RNA (New York, N.Y.) 52 18648070
2008 Scavenger receptor collectin placenta 1 (CL-P1) predominantly mediates zymosan phagocytosis by human vascular endothelial cells. The Journal of biological chemistry 52 19073604
2006 The phylogenetically conserved molluscan chitinase-like protein 1 (Cg-Clp1), homologue of human HC-gp39, stimulates proliferation and regulates synthesis of extracellular matrix components of mammalian chondrocytes. The Journal of biological chemistry 50 16882657
2006 Phospho-regulation of the Cdc14/Clp1 phosphatase delays late mitotic events in S. pombe. Developmental cell 48 16950131
2010 The Ustilago maydis Clp1 protein orchestrates pheromone and b-dependent signaling pathways to coordinate the cell cycle and pathogenic development. The Plant cell 47 20729384
2013 Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase. Molecular & cellular proteomics : MCP 45 23297348
2014 CLP1 as a novel player in linking tRNA splicing to neurodegenerative disorders. Wiley interdisciplinary reviews. RNA 44 25142875
2021 The Clp1 R140H mutation alters tRNA metabolism and mRNA 3' processing in mouse models of pontocerebellar hypoplasia. Proceedings of the National Academy of Sciences of the United States of America 36 34548404
2007 Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas. The FEBS journal 31 17608806
1996 AF10 is split by MLL and HEAB, a human homolog to a putative Caenorhabditis elegans ATP/GTP-binding protein in an invins(10;11)(p12;q23q12). Blood 30 8896421
2011 The interaction of Pcf11 and Clp1 is needed for mRNA 3'-end formation and is modulated by amino acids in the ATP-binding site. Nucleic acids research 29 21993299
2008 Expression and tissue localization of collectin placenta 1 (CL-P1, SRCL) in human tissues. Molecular immunology 29 18423602
2014 The putative cellodextrin transporter-like protein CLP1 is involved in cellulase induction in Neurospora crassa. The Journal of biological chemistry 28 25398875
2009 Characterization of a thermostable archaeal polynucleotide kinase homologous to human Clp1. RNA (New York, N.Y.) 28 19299550
2011 An essential role for Clp1 in assembly of polyadenylation complex CF IA and Pol II transcription termination. Nucleic acids research 27 21993300
2011 The P-loop domain of yeast Clp1 mediates interactions between CF IA and CPF factors in pre-mRNA 3' end formation. PloS one 27 22216186
2007 Pivotal role of cardiac lineage protein-1 (CLP-1) in transcriptional elongation factor P-TEFb complex formation in cardiac hypertrophy. Cardiovascular research 27 17459355
2002 Structure, expression, and functional characterization of the mouse CLP-1 gene. Gene 27 12119119
2007 Sexual development in Cryptococcus neoformans requires CLP1, a target of the homeodomain transcription factors Sxi1alpha and Sxi2a. Eukaryotic cell 24 17993575
2021 Functional analyses of Pleurotus ostreatus pcc1 and clp1 using CRISPR/Cas9. Fungal genetics and biology : FG & B 22 34153439
2012 Cardiac lineage protein-1 (CLP-1) regulates cardiac remodeling via transcriptional modulation of diverse hypertrophic and fibrotic responses and angiotensin II-transforming growth factor β (TGF-β1) signaling axis. The Journal of biological chemistry 21 22308025
2014 RNA specificity and regulation of catalysis in the eukaryotic polynucleotide kinase Clp1. Molecular cell 20 24813946
2016 Three pentraxins C-reactive protein, serum amyloid p component and pentraxin 3 mediate complement activation using Collectin CL-P1. Biochimica et biophysica acta. General subjects 19 27864148
2009 A link between aurora kinase and Clp1/Cdc14 regulation uncovered by the identification of a fission yeast borealin-like protein. Molecular biology of the cell 19 19570910
2016 Collectin CL-P1 utilizes C-reactive protein for complement activation. Biochimica et biophysica acta 18 26922829
2011 Molecular cloning and functional analysis of scavenger receptor zebrafish CL-P1. Biochimica et biophysica acta 18 22001438
2007 hClp1: a novel kinase revitalizes RNA metabolism. Cell cycle (Georgetown, Tex.) 16 17786051
2014 Scavenger receptor CL-P1 mainly utilizes a collagen-like domain to uptake microbes and modified LDL. Biochimica et biophysica acta 15 25199873
2020 Aire-dependent genes undergo Clp1-mediated 3'UTR shortening associated with higher transcript stability in the thymus. eLife 14 32338592
2010 CLP-1 associates with MyoD and HDAC to restore skeletal muscle cell regeneration. Journal of cell science 14 20940258
2021 RNA kinase CLP1/Cbc regulates meiosis initiation in spermatogenesis. Human molecular genetics 13 33864361
2020 Reduced Prenatal Pulmonary Lymphatic Function Is Observed in Clp1 Embryos With Impaired Motor Functions Including Fetal Breathing Movements in Preparation of the Developing Lung for Inflation at Birth. Frontiers in bioengineering and biotechnology 13 32211389
2008 The Flp1/Clp1 phosphatase cooperates with HECT-type Pub1/2 protein-ubiquitin ligases in Schizosaccharomyces pombe. Cell cycle (Georgetown, Tex.) 13 18418059
2006 S. pombe FEAR protein orthologs are not required for release of Clp1/Flp1 phosphatase from the nucleolus during mitosis. Journal of cell science 13 17032733
2012 Multiple protein kinases influence the redistribution of fission yeast Clp1/Cdc14 phosphatase upon genotoxic stress. Molecular biology of the cell 12 22918952
2012 Genome sequence of the phage clP1, which infects the beer spoilage bacterium Pediococcus damnosus. Gene 11 22564705
2021 Modeling a human CLP1 mutation in mouse identifies an accumulation of tyrosine pre-tRNA fragments causing pontocerebellar hypoplasia type 10. Biochemical and biophysical research communications 9 34273619
2014 Small double-stranded RNA mediates the anti-cancer effects of p21WAF1/ClP1 transcriptional activation in a human glioma cell line. Yonsei medical journal 9 24532499
2009 Down-regulation of cardiac lineage protein (CLP-1) expression in CLP-1 +/- mice affords. Journal of cellular and molecular medicine 9 18624753
2011 The induction of human CL-P1 expression in hypoxia/reoxygenation culture condition and rat CL-P1 after ischemic/reperfusion treatment. Biochimica et biophysica acta 8 21723916
2022 The Plant Homeodomain Protein Clp1 Regulates Fungal Development, Virulence, and Autophagy Homeostasis in Magnaporthe oryzae. Microbiology spectrum 6 36036638
2019 Large-Scale Molecular Evolutionary Analysis Uncovers a Variety of Polynucleotide Kinase Clp1 Family Proteins in the Three Domains of Life. Genome biology and evolution 6 31513263
2014 Scavenger receptor CL-P1 mediates endocytosis by associating with AP-2μ2. Biochimica et biophysica acta 6 25109811
2003 Haplotype analysis of the human collectin placenta 1 (hCL-P1) gene. Journal of human genetics 6 12601552
2020 CLP1 acts as the main RNA kinase in mice. Biochemical and biophysical research communications 4 32081435
2024 The Cdc14 phosphatase, Clp1, does not affect genome expression. microPublication biology 1 38415071
2023 Systematic Analysis of Diverse Polynucleotide Kinase Clp1 Family Proteins in Eukaryotes: Three Unique Clp1 Proteins of Trypanosoma brucei. Journal of molecular evolution 1 37606665
2023 Fission yeast Cdc14-like phosphatase Flp1/Clp1 modulates the transcriptional response to oxidative stress. Scientific reports 1 37674027
2024 Deciphering significant interaction between Clp1 (CF IA) and Ssu72 (CPF) in pre-mRNA processing via in silico approaches. Journal of biomolecular structure & dynamics 0 39522172

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