Affinage

NRBP1

Nuclear receptor-binding protein · UniProt Q9UHY1

Length
535 aa
Mass
59.8 kDa
Annotated
2026-04-29
48 papers in source corpus 19 papers cited in narrative 19 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NRBP1 is a pseudokinase that functions as a multivalent adaptor/scaffold protein, coupling ubiquitin-dependent protein degradation, small GTPase signaling, and osmosensing kinase activation to diverse cellular processes including cell growth, volume regulation, and retrotransposon control. NRBP1 assembles a heterodimeric Cullin-RING ubiquitin ligase via its BC-box and cryptic H-box, recruiting Cul2/Cul4A to target substrates such as BRI2, BRI3, and SALL4 for proteasomal degradation, with TSC22D proteins acting as chaperone-like enhancers of CRL formation (PMID:32160551, PMID:31864704). Through its CCT-like domain, NRBP1 binds TSC22D proteins and directly activates WNK4 kinase, assembling with WNK1, TSC22D, and SPAK into biomolecular condensates that form within seconds of hyperosmotic stress to drive WNK-SPAK/OSR1 signaling and cell volume regulation; kidney-specific knockout reduces NCC phosphorylation and Na⁺ reabsorption in the distal convoluted tubule (PMID:40668933, PMID:40668923, PMID:38980795). NRBP1 also scaffolds a P-Rex1–Rac1–Cdc42 signaling complex to activate Rac1/Cdc42 GTPase-dependent migration and invasion, regulates LINE1 retrotransposition through the L1 ribonucleoprotein complex (opposed by NRBP2-induced proteasomal degradation of NRBP1), and its own stability is controlled by TRIM24-mediated ubiquitination at K430 requiring prior S42 phosphorylation (PMID:36693952, PMID:40645931, PMID:41430038).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 2000 Medium

    The initial cloning of NRBP1 revealed its multidomain architecture—including a kinase-like domain, LXXLL motifs, SH2-binding regions, and PEST sequences—establishing it as a candidate adaptor/pseudokinase rather than a conventional signaling kinase.

    Evidence cDNA cloning, domain analysis, and in vitro translation showing three protein products

    PMID:10843813

    Open questions at the time
    • No binding partners or biological function identified
    • Kinase activity not tested directly
    • In vivo relevance unknown
  2. 2002 High

    Two independent studies identified NRBP1's first physical interaction partners—activated Rac3 GTPase and the Mlf1 oncoprotein—and showed that NRBP1 recruits an associated serine kinase, establishing it as an interaction hub with functional consequences for Golgi transport and myeloid differentiation.

    Evidence Yeast two-hybrid, co-immunoprecipitation with Rac3 and Mlf1, Golgi redistribution assay, in vitro kinase assay, and M1 myeloid differentiation suppression

    PMID:11956649 PMID:12176995

    Open questions at the time
    • Identity of the recruited kinase unknown
    • Mechanism of Golgi transport regulation unresolved
    • Whether Rac3 interaction is direct or scaffold-mediated unclear
  3. 2006 Medium

    Identification of Jab1 (CSN5) as an NRBP1 interactor revealed that NRBP1 negatively regulates AP-1 transcriptional activation by blocking Jab1-mediated c-Jun phosphorylation, placing NRBP1 in a signaling-suppressive role.

    Evidence Co-immunoprecipitation and AP-1 reporter assay with c-Jun phosphorylation readout

    PMID:17052710

    Open questions at the time
    • Mechanism of Jab1 inhibition (competitive binding vs. sequestration) not resolved
    • Physiological relevance in endogenous settings untested
    • No loss-of-function data
  4. 2010 High

    Drosophila genetic studies established that the NRBP1 ortholog Madm physically and functionally partners with long TSC22 domain proteins (Bunched A) to promote organ growth, identifying the TSC22D–NRBP axis as an evolutionarily conserved growth-regulatory complex.

    Evidence Proteomics, co-IP, genetic epistasis, and overexpression phenotypes in Drosophila

    PMID:20149264

    Open questions at the time
    • Mammalian TSC22D–NRBP1 growth function not yet demonstrated
    • Downstream effectors of the complex unknown
    • Whether the complex has catalytic activity unresolved
  5. 2015 Medium

    Epistasis experiments in Drosophila intestinal stem cells placed Madm/NRBP1 downstream of TOR signaling, showing that Madm and Bunched are required for TOR-dependent growth and 4E-BP phosphorylation.

    Evidence MARCM clonal analysis, cell-type-specific RNAi, epistasis with TSC/Rheb, 4E-BP phosphorylation assay

    PMID:26323255

    Open questions at the time
    • Direct biochemical mechanism linking Madm to TOR effectors unknown
    • Whether this pathway is conserved in mammals not tested
    • Single tissue system
  6. 2016 Medium

    Drosophila studies revealed that Madm/NRBP1 acts as a tumour suppressor in testis stem cell niche competition through regulation of EGFR ligand expression, with JAK signaling promoting its nuclear translocation to suppress EGF receptor pathway activity.

    Evidence RNAi, genetic epistasis, immunofluorescence showing nuclear translocation, EGFR signaling assays

    PMID:26792023

    Open questions at the time
    • Mechanism of NRBP1 nuclear translocation by JAK unclear
    • Conservation of this tumor-suppressive role in mammals not shown
    • Direct transcriptional target regulation not demonstrated
  7. 2019 Medium

    The discovery that NRBP1 induces SALL4 ubiquitination and degradation—antagonized by THG-1—revealed NRBP1 as a ubiquitin-pathway component controlling stemness transcription factor stability in cancer.

    Evidence Co-IP, ubiquitination assay, knockdown, tumorsphere assay in esophageal squamous cell carcinoma

    PMID:31864704

    Open questions at the time
    • E3 ligase complex mediating SALL4 ubiquitination not identified at that time
    • Structural basis of THG-1 competition unknown
    • Generalizability across cancer types unclear
  8. 2020 High

    A comprehensive interactome study resolved a central mechanistic question by showing NRBP1 is a bona fide substrate receptor for Cullin-RING E3 ligase complexes, assembling a heterodimeric CRL through BC-box/H-box interactions with Cul2 and Cul4A to target BRI2/BRI3 for degradation, with TSC22D3/D4 acting as chaperone-like enhancers of CRL assembly.

    Evidence Mass spectrometry interactome, reciprocal co-IP, ubiquitin ligase assay, siRNA knockdown in neuronal cells, Aβ ELISA

    PMID:32160551

    Open questions at the time
    • Full substrate repertoire of NRBP1-CRL unknown
    • Whether BRI2/BRI3 degradation is relevant in vivo in Alzheimer's disease not tested
    • Structural basis of NRBP1 dimerization in CRL unresolved
  9. 2021 Medium

    Placement of NRBP1 in a microglial ERK-CREB-BDNF signaling axis expanded its functional repertoire to neuroinflammation and synaptic plasticity, with ERK activation upregulating NRBP1 expression in microglia.

    Evidence iTRAQ proteomics, primary microglia culture, immunofluorescence, CREB/MeCP2 inhibition, CSF1R depletion

    PMID:34819637

    Open questions at the time
    • Direct molecular role of NRBP1 in CREB activation not defined
    • Whether NRBP1 is required vs. sufficient for BDNF induction unclear
    • Mechanism linking ERK to NRBP1 expression unknown
  10. 2022 High

    Drosophila NMJ studies demonstrated that Madm/NRBP1 coordinates synaptic growth and stability by controlling TOR-dependent translation via 4E-BP and S6K, and revealed a transsynaptic homeostatic signaling mechanism dependent on postsynaptic Madm.

    Evidence Cell-type-specific RNAi, genetic epistasis, NMJ electrophysiology, 4E-BP phosphorylation, presynaptic homeostatic potentiation assay

    PMID:36450258

    Open questions at the time
    • Identity of the transsynaptic signal unknown
    • Conservation at mammalian synapses untested
    • Whether NRBP1 directly regulates 4E-BP or acts indirectly through TOR unclear
  11. 2023 High

    BioID profiling identified P-Rex1 as an NRBP1 binding partner, establishing NRBP1 as a scaffold for a P-Rex1–Rac1–Cdc42 signaling complex that activates small GTPases to drive migration, invasion, and ROS-dependent proliferation in triple-negative breast cancer.

    Evidence BioID/MS proximity labeling, co-IP, GTP-Rac1/Cdc42 pull-down, siRNA, constitutively active Rac1 rescue, xenograft

    PMID:36693952

    Open questions at the time
    • How NRBP1 activates P-Rex1 GEF activity mechanistically unknown
    • Whether other Rho-family GTPases are regulated not tested
    • Structural basis of the NRBP1–P-Rex1 interaction unresolved
  12. 2024 High

    The discovery that TSC22D, WNK, and NRBP1 families co-assemble into biomolecular condensates within seconds of hyperosmotic stress—dependent on intrinsically disordered regions—revealed a phase-separation-based osmosensing mechanism that co-evolved in metazoans via the NbrT domain of NRBP1.

    Evidence Co-essentiality analysis, live-cell imaging of condensates, co-IP, IDR deletion mutants, phylogenetic analysis

    PMID:38980795

    Open questions at the time
    • Biophysical properties of condensates (material state, dynamics) not fully characterized
    • Whether condensate formation is required vs. correlated with WNK activation not formally demonstrated
    • Contribution of individual IDRs to phase behavior unclear
  13. 2025 High

    Three independent 2025 studies resolved major mechanistic questions: NRBP1 directly activates WNK4 kinase in vitro and is required for basal and osmotic WNK-SPAK signaling with kidney-specific KO reducing NCC phosphorylation; NRBP1 regulates LINE1 retrotransposition via the L1 RNP complex with NRBP2 opposing it through proteasomal degradation of NRBP1; and TRIM24 controls NRBP1 stability through ubiquitination at K430 requiring S42 phosphorylation.

    Evidence In vitro WNK4 activation assay, CRISPR KO and tissue-specific KO mice, L1 retrotransposition reporter, heterodimer formation/degradation assay, TRIM24 co-IP with site-directed mutagenesis

    PMID:40645931 PMID:40668923 PMID:40668933 PMID:41430038

    Open questions at the time
    • Whether NRBP1 activates WNK4 through allosteric vs. scaffolding mechanism not structurally resolved
    • Physiological role of NRBP1 in LINE1 regulation in vivo unknown
    • Cross-talk between TRIM24-mediated degradation and NRBP2-mediated degradation pathways not explored

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include how NRBP1's multiple scaffolding functions (CRL substrate receptor, WNK activator, P-Rex1 scaffold, L1 RNP component) are coordinated or compete for NRBP1 pools, whether biomolecular condensate formation is mechanistically required for WNK pathway activation or is a correlate, and what the full substrate repertoire of the NRBP1-CRL complex encompasses in vivo.
  • No integrated model of how NRBP1 partitions across its multiple functions
  • Structural basis of NRBP1 pseudokinase domain function and CCT-like domain interactions awaits high-resolution experimental structures
  • In vivo relevance of NRBP1 in human disease (neurodegeneration, cancer) not established through genetic studies

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 4 GO:0098772 molecular function regulator activity 4 GO:0140096 catalytic activity, acting on a protein 2
Localization
GO:0005829 cytosol 4 GO:0005634 nucleus 2 GO:0005794 Golgi apparatus 1
Pathway
R-HSA-162582 Signal Transduction 4 R-HSA-392499 Metabolism of proteins 4 R-HSA-382551 Transport of small molecules 3
Partners
CUL2CUL4ANRBP2PREX1TSC22D2TSC22D4WNK1WNK4
Complex memberships
NRBP1-Cul2/Cul4A CRLNRBP1-P-Rex1-Rac1-Cdc42TSC22D-WNK-NRBP1-SPAK condensate

Evidence

Reading pass · 19 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 NRBP1 (NRBP) was identified as a multidomain putative adapter protein containing two putative nuclear receptor binding motifs (LXXLL), a putative SH2 domain-binding region, a kinase-like domain, a bipartite nuclear localization signal, and PEST sequences. In vitro translation revealed three products (60, 51, 43 kDa), suggesting multiple translation initiation sites. cDNA cloning, in vitro translation, domain analysis Genomics Medium 10843813
2002 NRBP1 physically interacts with activated Rac3 GTPase (constitutively active V12Rac3) and co-localizes with it at endomembranes and lamellipodia. NRBP1 overexpression caused dramatic redistribution of Golgi marker p58, suggesting a role in ER-to-Golgi transport. NRBP contains an associated kinase activity. Yeast two-hybrid, co-immunoprecipitation, immunocytochemistry, Golgi redistribution assay International journal of molecular medicine Medium 11956649
2002 NRBP1 (Madm) was identified as a binding partner of Mlf1 oncoprotein via yeast two-hybrid; Madm co-immunoprecipitated with Mlf1, co-localized in the cytoplasm, and recruited a serine kinase that phosphorylated both Madm and Mlf1 at the RSXSXP 14-3-3 binding motif. Ectopic Madm expression in M1 myeloid cells suppressed cytokine-induced differentiation. Yeast two-hybrid, co-immunoprecipitation, in vitro kinase assay, ectopic expression/differentiation assay The Journal of biological chemistry High 12176995
2006 NRBP1 interacts in vivo with Jab1 (COP9 signalosome component 5) and inhibits Jab1-induced phosphorylation of c-Jun and AP-1 transcriptional activation. Overexpression of NRBP1 specifically inhibits AP-1 activation by various stimuli. Co-immunoprecipitation, AP-1 reporter assay, c-Jun phosphorylation assay FEBS letters Medium 17052710
2010 Drosophila Madm (NRBP1 ortholog) physically associates with the long TSC22 domain protein Bunched A (BunA) via a conserved motif present only in long TSC22DF isoforms, forming a growth-regulating complex. Genetic and biochemical evidence shows Madm and BunA synergize to promote organ growth. Proteomics, co-immunoprecipitation, genetic screen, genetic epistasis, overexpression phenotype Journal of biology High 20149264
2015 Drosophila Madm acts downstream of the TOR pathway (epistasis with Tuberous Sclerosis Complex/Rheb) to regulate growth and division of intestinal stem cells; loss of Bunched or Madm suppressed elevated cell growth and 4EBP phosphorylation induced by TSC loss or Rheb overexpression. Bunched (Madm partner) was shown to function in the cytoplasm for this role. MARCM clonal analysis, cell-type-specific RNAi, genetic epistasis, 4EBP phosphorylation assay Stem cell reviews and reports Medium 26323255
2016 Drosophila Madm acts as a tumour suppressor in testis stem cell competition; Madm knockdown leads to overexpression of EGF receptor ligand vein (vn), activating EGF receptor signalling and integrin expression non-cell-autonomously in cyst stem cells. Constitutively active JAK (hop(Tum-l)) promotes Madm nuclear translocation and suppresses vn/integrin expression. RNAi knockdown, genetic epistasis, immunofluorescence (nuclear translocation), EGF receptor signaling assays Nature communications Medium 26792023
2019 NRBP1 induces ubiquitination of the stemness transcription factor SALL4, promoting its degradation. THG-1 (a competing binding protein) antagonizes NRBP1 binding to SALL4, preventing SALL4 ubiquitination and stabilizing it to promote stemness gene expression (NANOG, OCT4) and tumorsphere growth in esophageal squamous cell carcinoma cells. Co-immunoprecipitation, ubiquitination assay, NRBP1/THG-1 knockdown, tumorsphere assay, rescue experiments Biochemical and biophysical research communications Medium 31864704
2020 NRBP1 functions as a substrate receptor of a Cullin-RING ubiquitin ligase (CRL) complex, targeting BRI2 and BRI3 (inhibitors of amyloid precursor protein processing) for degradation. Dimerized NRBP1 assembles a functional heterodimeric CRL through a BC-box and an overlapping cryptic H-box interacting with both Cul2 and Cul4A. Chaperone-like activity of TSC22D3 and TSC22D4 strongly enhances NRBP1 CRL formation. NRBP1 knockdown in neuronal cells increases BRI2/BRI3 abundance and reduces Aβ production. Mass spectrometry interactome, co-immunoprecipitation, ubiquitin ligase assay, siRNA knockdown, Aβ ELISA Cell reports High 32160551
2021 NRBP1 is localized in microglia and neurons (not astrocytes) of mouse medial prefrontal cortex. (R)-ketamine increases NRBP1 expression in primary microglia through ERK activation, and NRBP1 operates in an ERK-NRBP1-CREB-BDNF signaling axis. (R)-ketamine activates BDNF transcription through CREB activation and MeCP2 suppression in microglia. iTRAQ proteomics, primary microglia culture, immunofluorescence localization, CREB/MeCP2 inhibition (HDO), CSF1R inhibitor microglial depletion, dendritic spine density assay Molecular psychiatry Medium 34819637
2022 Drosophila Madm/NRBP1 is required presynaptically to maintain synaptic stability and coordinates synaptic growth and function by controlling cap-dependent translation via the TOR effector 4E-BP/Thor. Postsynaptic Madm induces a compensatory transsynaptic signal utilizing the presynaptic homeostatic potentiation (PHP) machinery via regulation of 4E-BP/Thor and S6-kinase, delaying synaptic degeneration. Cell-type-specific RNAi, genetic epistasis, electrophysiology (NMJ), 4E-BP phosphorylation assay, presynaptic homeostatic potentiation assay Cell reports High 36450258
2023 NRBP1 pseudokinase binds P-Rex1 (a guanine nucleotide exchange factor for Rac1) as identified by BioID/MS profiling, and acts as a scaffold for a complex with P-Rex1, Rac1, and Cdc42. NRBP1 overexpression enhances GTP-bound Rac1 and Cdc42 levels in a P-Rex1-dependent manner, promoting TNBC cell migration, invasion, and proliferation via reactive oxygen species generation. BioID/MS proximity labeling, co-immunoprecipitation, GTP-Rac1/Cdc42 pull-down assay, siRNA knockdown, constitutively active Rac1 rescue, ROS assay, xenograft Oncogene High 36693952
2024 TSC22D, WNK, and NRBP1 family members physically associate into biomolecular condensates within seconds of hyperosmotic stress, dependent on intrinsically disordered regions (IDRs). These protein families co-evolved in metazoans (TSC22D genes evolved alongside a domain in NRBPs, termed NbrT, that specifically binds TSC22D proteins) to co-regulate rapid cell volume changes in response to osmolarity. Gene co-essentiality analysis, live-cell imaging of condensate formation, co-immunoprecipitation, IDR deletion mutants, phylogenetic analysis Cell reports High 38980795
2025 NRBP1 pseudokinase directly activates WNK4 kinase in vitro, and knockdown/knockout of NRBP1 markedly inhibits basal and sorbitol-induced activation of WNK1 and downstream SPAK/OSR1 components. NRBP1 contains a CCT-like domain that, together with TSC22D4 RΦ-motifs, is predicted (AlphaFold-3) to form a complex with WNK1 and SPAK. Osmotic stress promotes association of WNK1 with NRBP1 and TSC22D2/4 confirmed by immunoprecipitation and mass spectrometry. Proximity labeling, co-immunoprecipitation, mass spectrometry, immunoblotting, siRNA/CRISPR knockout, in vitro WNK4 kinase activation assay, AlphaFold-3 structural modeling Science advances High 40668933
2025 DCT-specific NRBP1 knockout mice show reduced NCC phosphorylation and activate a compensatory response, demonstrating that NRBP1 and long TSC22D proteins are positive modulators of WNK signaling that regulate Na+ reabsorption in the distal convoluted tubule kidney. TSC22D1.1, TSC22D2, and NRBP1 co-localize in DCT WNK bodies (cytoplasmic biomolecular condensates associated with WNK activation). Tissue-specific knockout mouse, NCC phosphorylation assay, immunofluorescence co-localization, HEK293 cell WNK4 activity assay Science advances High 40668923
2025 NRBP1 and its paralog NRBP2 oppositely regulate LINE1 (L1) retrotransposition by influencing integrity of the L1 ribonucleoprotein complex. NRBP2 targets NRBP1 for proteasome-mediated degradation, likely through heterodimer formation, accounting for their opposing roles rather than competition for shared binding partners. L1 retrotransposition reporter assay, co-immunoprecipitation, proteasome inhibitor rescue, heterodimer formation assay, phylogenetic analysis Nature communications High 40645931
2025 TRIM24 E3 ubiquitin ligase binds NRBP1, enhances its ubiquitination and subsequent degradation. NRBP1 phosphorylation at residue S42 is required for TRIM24-mediated ubiquitination, and K430 is the specific ubiquitination site targeted by TRIM24. Co-immunoprecipitation, ubiquitination assay, site-directed mutagenesis (S42, K430), siRNA knockdown, Western blotting Cell death & disease High 41430038
2024 The NRBP1 CCT-like domain binds TSC22D1 via the same RΦ-motif as OSR1 and SPAK, including variants lacking the conserved arginine previously thought to be essential, revealing that NRBP1 participates in the WNK signaling network through CCT domain-mediated protein interactions. Motif interaction analysis, biochemical binding assays, CCT domain characterization bioRxiv (preprint)preprint Medium bio_10.1101_2024.06.26.600905
2017 DNA methylation at the NRBP1 promoter region (72 bp upstream of TSS, site B1) increases binding of transcription factor TFAP2A, leading to suppressed NRBP1 expression. Hypomethylation reduces TFAP2A binding and elevates NRBP1 expression, as shown in gout patient PBMCs and in vitro. Luciferase reporter assay, protein pulldown assay, bisulfite pyrosequencing Clinical epigenetics Medium 28932319

Source papers

Stage 0 corpus · 48 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2021 Microglial ERK-NRBP1-CREB-BDNF signaling in sustained antidepressant actions of (R)-ketamine. Molecular psychiatry 171 34819637
1998 The metallo-disintegrin ADAM10 (MADM) from bovine kidney has type IV collagenase activity in vitro. Biochemical and biophysical research communications 106 9571200
1996 Molecular cloning of MADM: a catalytically active mammalian disintegrin-metalloprotease expressed in various cell types. The Biochemical journal 101 8694785
2007 Modeling sporadic loss of heterozygosity in mice by using mosaic analysis with double markers (MADM). Proceedings of the National Academy of Sciences of the United States of America 64 17360552
2021 A genome-wide library of MADM mice for single-cell genetic mosaic analysis. Cell reports 49 34161767
2012 Extensions of MADM (mosaic analysis with double markers) in mice. PloS one 47 22479386
2002 MADM, a novel adaptor protein that mediates phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1. The Journal of biological chemistry 47 12176995
2020 NRBP1-Containing CRL2/CRL4A Regulates Amyloid β Production by Targeting BRI2 and BRI3 for Degradation. Cell reports 34 32160551
2000 Cloning of the cDNA and localization of the gene encoding human NRBP, a ubiquitously expressed, multidomain putative adapter protein. Genomics 32 10843813
2010 Madm (Mlf1 adapter molecule) cooperates with Bunched A to promote growth in Drosophila. Journal of biology 31 20149264
2017 DNA hypomethylation of a transcription factor binding site within the promoter of a gout risk gene NRBP1 upregulates its expression by inhibition of TFAP2A binding. Clinical epigenetics 28 28932319
2002 Interaction of the small GTPase Rac3 with NRBP, a protein with a kinase-homology domain. International journal of molecular medicine 26 11956649
2016 The novel tumour suppressor Madm regulates stem cell competition in the Drosophila testis. Nature communications 24 26792023
2012 High NRBP1 expression in prostate cancer is linked with poor clinical outcomes and increased cancer cell growth. The Prostate 24 22473923
2015 NRBP1 is downregulated in breast cancer and NRBP1 overexpression inhibits cancer cell proliferation through Wnt/β-catenin signaling pathway. OncoTargets and therapy 23 26715855
2013 MADM-ML, a mouse genetic mosaic system with increased clonal efficiency. PloS one 22 24143253
2020 Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM). Journal of visualized experiments : JoVE 19 32449730
2006 Adapter protein NRBP associates with Jab1 and negatively regulates AP-1 activity. FEBS letters 18 17052710
2024 The TSC22D, WNK, and NRBP gene families exhibit functional buffering and evolved with Metazoa for cell volume regulation. Cell reports 14 38980795
2020 Puerarin Inhibits the Progression of Bladder Cancer by Regulating circ_0020394/miR-328-3p/NRBP1 Axis. Cancer biotherapy & radiopharmaceuticals 13 33016781
2018 MiR-519d inhibits prostate cancer cell proliferation, cycle and invasion via targeting NRBP1. European review for medical and pharmacological sciences 13 29863241
2023 The pseudokinase NRBP1 activates Rac1/Cdc42 via P-Rex1 to drive oncogenic signalling in triple-negative breast cancer. Oncogene 12 36693952
2021 CircLRP6 contributes to prostate cancer growth and metastasis by binding to miR-330-5p to up-regulate NRBP1. World journal of surgical oncology 12 34158077
2019 THG-1 suppresses SALL4 degradation to induce stemness genes and tumorsphere formation through antagonizing NRBP1 in squamous cell carcinoma cells. Biochemical and biophysical research communications 11 31864704
2014 Mosaic analysis with double markers (MADM) in mice. Cold Spring Harbor protocols 10 24492775
2014 Generation and applications of MADM-based mouse genetic mosaic system. Methods in molecular biology (Clifton, N.J.) 10 25064104
2024 NRBP1 promotes malignant phenotypes of glioblastoma by regulating PI3K/Akt activation. Cancer medicine 8 39149873
2022 NRBP1 negatively regulates SALL4 to reduce the invasion and migration, promote apoptosis and increase the sensitivity to chemotherapy drugs of breast cancer cells. Oncology letters 8 35317026
2021 Circular RNA circ_0062019 exerts oncogenic properties in prostate cancer via mediating miR-1253/NRBP1 axis. Andrologia 8 34866220
2022 NRBP1 modulates uric acid transporter ABCG2 expression by activating the Wnt/β-catenin pathway in HK-2 cells. Nefrologia 7 36437206
2015 Bunched and Madm Function Downstream of Tuberous Sclerosis Complex to Regulate the Growth of Intestinal Stem Cells in Drosophila. Stem cell reviews and reports 5 26323255
2023 The Circular RNA Circ_0085494 Regulates Prostate Cancer Progression Through NRBP1/miR-497-5p Axis. Biochemical genetics 4 36790665
2021 Detection and classification of neurons and glial cells in the MADM mouse brain using RetinaNet. PloS one 4 34559842
2025 NRBP1 pseudokinase binds to and activates the WNK pathway in response to osmotic stress. Science advances 3 40668933
2022 Performing Single-Cell Clonal Analysis in the Mouse Brain Using Mosaic Analysis with Double Markers (MADM). Methods in molecular biology (Clifton, N.J.) 3 35776345
2025 NRBP1 and TSC22D proteins affect distal convoluted tubule physiology through modulation of the WNK pathway. Science advances 2 40668923
2022 Madm/NRBP1 mediates synaptic maintenance and neurodegeneration-induced presynaptic homeostatic potentiation. Cell reports 2 36450258
2021 A novel MADM algorithm for landfill site selection based on q-rung orthopair probabilistic hesitant fuzzy power Muirhead mean operator. PloS one 2 34673792
2025 Silencing NRBP1 Gene with shRNA Improves Cognitive Function and Pathological Features in AD Rat Model. Biochemical genetics 1 40616751
2024 Protocol for mapping cell lineage and cell-type identity of clonally-related cells in situ using MADM-CloneSeq. STAR protocols 1 38968076
2024 NRBP1 and TSC22D proteins impact distal convoluted tubule physiology through modulation of the WNK pathway. bioRxiv : the preprint server for biology 1 39764004
2022 Protocol to identify small molecules promoting rat and mouse cardiomyocyte proliferation based on the FUCCI and MADM reporters. STAR protocols 1 36595956
2026 DNA Methylation Regulates CDK5R1 and NRBP1 to Exert Effects on Alcohol Dependence: Insights From Mendelian Randomization. Addiction biology 0 42025296
2025 Probing Cell-Type Specificity of Mutant Phenotype at Transcriptomic Level Using Mosaic Analysis with Double Markers (MADM). Methods in molecular biology (Clifton, N.J.) 0 39745639
2025 In Vivo Clonal Analysis Using MADM with Spatiotemporal Specificity. Methods in molecular biology (Clifton, N.J.) 0 39745645
2025 Opposing roles of pseudokinases NRBP1 and NRBP2 in regulating L1 retrotransposition. Nature communications 0 40645931
2025 WNK kinase activity is modulated by the pseudokinase NRBP1 and the scaffold proteins of the TSC22D family. Current opinion in nephrology and hypertension 0 40726374
2025 TRIM24 promotes proliferation and metastasis of gastric cancer via mediating NRBP1 ubiquitination. Cell death & disease 0 41430038