Affinage

NOX1

NADPH oxidase 1 · UniProt Q9Y5S8

Length
564 aa
Mass
64.9 kDa
Annotated
2026-04-29
100 papers in source corpus 40 papers cited in narrative 40 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NOX1 is a superoxide-generating NADPH oxidase that functions as a catalytic core of a multi-subunit complex to produce reactive oxygen species (ROS) serving as second messengers in cell proliferation, migration, vascular tone regulation, innate epithelial defense, and stem cell self-renewal. The active complex requires the membrane partner p22phox for maturation and activity, cytosolic organizers NOXO1 (or Tks4/Tks5), the activator NOXA1, and the small GTPase Rac1, which is recruited via PI3K→βPix signaling; PKCβ1 phosphorylation of NOX1 at Thr429 controls NOXA1 docking and complex assembly (PMID:12473664, PMID:15477006, PMID:27614387, PMID:15121857, PMID:25228390). NOX1-derived superoxide is dismutated to H₂O₂, which oxidatively inactivates phosphatases PTEN and PTP-PEST to activate FAK-dependent migration, drives EGFR/MAPK/AKT signaling cascades promoting proliferation and transformation, and oxidizes NMDA receptor NR1 at C744 to modulate neuronal BDNF expression (PMID:11331784, PMID:23241962, PMID:20525691, PMID:28314819). In vivo, Nox1 deficiency attenuates angiotensin II-induced hypertension by preserving NO bioavailability, impairs mucosal wound repair, reduces pulmonary arterial smooth muscle cell apoptosis through loss of Kv1.5 channel regulation, and compromises colonic stem cell proliferation downstream of TLR–EGFR signaling; loss-of-function NOX1 variants are associated with very-early-onset inflammatory bowel disease and defective epithelial antimicrobial defense (PMID:16246966, PMID:23241962, PMID:24233492, PMID:33826887, PMID:26301257).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 1999 High

    Identification of NOX1 as a non-phagocytic gp91phox homologue established that dedicated NADPH oxidases exist outside immune cells and that their superoxide output drives mitogenic signaling and transformation.

    Evidence Overexpression/antisense in NIH3T3 and VSMCs with superoxide measurement and tumorigenicity assays

    PMID:10485709

    Open questions at the time
    • Accessory subunits not yet identified
    • Endogenous physiological stimuli undefined
    • In vivo relevance unconfirmed
  2. 2001 High

    Demonstrating that H₂O₂ (from superoxide dismutation) is the actual intracellular second messenger resolved whether superoxide or its derivative mediates NOX1 downstream signaling, and separately established that NOX1 mediates Ang II- and PDGF-induced redox signaling to p38 MAPK and Akt in vascular smooth muscle cells.

    Evidence Catalase co-expression reversing transformation and gene expression; antisense adenoviral knockdown blocking Ang II-induced signaling in VSMCs

    PMID:11331784 PMID:11348997

    Open questions at the time
    • The cytosolic activator subunits required for NOX1 enzymatic activity were still unknown
    • Source of vascular ROS in vivo not yet demonstrated genetically
  3. 2002 High

    Discovery of the dedicated organizer NOXO1 and activator NOXA1 — distinct from phagocyte p47phox/p67phox — explained why NOX1 can be constitutively active and defined the minimal subunit complement for reconstituted activity.

    Evidence cDNA cloning plus reconstitution: co-expression of NOX1/NOXO1/NOXA1 yields constitutive superoxide; domain-swap with p47phox restores stimulus-dependence

    PMID:12473664

    Open questions at the time
    • Role of small GTPases in the complex not yet defined
    • p22phox requirement for NOX1 not formally demonstrated
  4. 2004 High

    Three converging studies established: (1) oncogenic Ras upregulates NOX1 via MEK-MAPK and NOX1 is required for Ras transformation; (2) PI3K→βPix→Rac1 constitutes the upstream activation cascade linking growth factors to NOX1; (3) p22phox physically associates with NOX1 at the plasma membrane and is required for superoxide output; and (4) bacterial LPS activates NOX1 via transcriptional upregulation plus Rac1-GTP loading.

    Evidence siRNA knockdown blocking Ras transformation; reciprocal co-IP of NOX1-βPix-Rac1 with siRNA epistasis; co-IP and confocal colocalization of NOX1-p22phox; Rac1 GTP-loading and CA-Rac1 rescue in gastric cells

    PMID:15121857 PMID:15150115 PMID:15469954 PMID:15477006

    Open questions at the time
    • Post-translational regulation of NOX1 itself not identified
    • In vivo vascular phenotype of Nox1 loss not yet tested
  5. 2005 High

    Genetic loss-of-function in vivo demonstrated that NOX1 is required for angiotensin II-induced superoxide production and hypertension, establishing NOX1 as a mediator of vascular tone through reduction of NO bioavailability.

    Evidence Nox1 knockout mice with telemetric blood pressure, aortic superoxide measurement, endothelium-dependent relaxation, and cGMP assays

    PMID:16246966

    Open questions at the time
    • Renal contribution of NOX1 to blood pressure not separated from vascular contribution
    • Downstream ion channel targets unknown
  6. 2007 High

    Multiple 2007 studies expanded the mechanistic picture: NOX1 was placed in a TNFα-induced necrosis complex with TRADD/RIP1/Rac1; transcriptional regulation was mapped to STAT1/3 (GAS elements), MEF2B (ATF-1 cascade), and GATA/Cdx/HNF-1α cooperativity at the promoter; NOX1 overexpression was shown to cause genome instability.

    Evidence Co-IP of TRADD/RIP1/NOX1/Rac1 complex validated by RIP1 deficiency; ChIP of STAT1/3 at NOX1 promoter; promoter deletion/ChIP for GATA-6/Cdx2/HNF-1α; mutagenesis rate assay

    PMID:17560373 PMID:17822438 PMID:17963706 PMID:18005670 PMID:19834108

    Open questions at the time
    • Post-translational activation mechanism of NOX1 by TNFα not defined
    • NOX1 role in epithelial innate defense not yet examined
  7. 2009 High

    Domain-swap chimeras between NOX1 and NOX4 revealed that the N-terminal transmembrane region dictates plasma membrane targeting and superoxide (vs. H₂O₂) release, while the C-terminal cytosolic tail confers subunit-dependent activation; separately, Tks4/Tks5 were identified as alternative NOX1 organizers that recruit NOX1 to invadopodia.

    Evidence NOX1/NOX4 chimeric constructs with TIRF microscopy and ROS measurement; Tks4 siRNA blocking invadopodia-localized ROS in DLD1 cells

    PMID:19061439 PMID:19755710

    Open questions at the time
    • Structural basis for subunit selectivity unresolved
    • Whether Tks and NOXO1 compete or cooperate in vivo unclear
  8. 2010 High

    Studies in 2010–2011 defined compartmentalized NOX1 activation (dynamin-dependent endosomal for TNFα vs. plasma membrane EGFR-transactivation for thrombin), established EGFR/TGF-α positive feedback, identified NOX1's role in hepatic stellate cell fibrosis, and revealed that NOX1 modulates opioid signaling and analgesic tolerance in dorsal spinal cord.

    Evidence Dominant-negative dynamin and shRNA defining compartmentalized signaling; NOX1 siRNA reducing EGFR/TGF-α autocrine loop; Nox1 KO with bone marrow chimeras in fibrosis models; Nox1 KO mice with GTPase/PKC/RGS9-2 biochemistry in morphine tolerance

    PMID:19737091 PMID:20525691 PMID:21384410 PMID:22159121

    Open questions at the time
    • Direct phosphorylation events on NOX1 controlling activation unknown
    • Mechanism of NOX1-mediated SIRT1 activation only partially defined
  9. 2012 High

    NOX1 was linked to Ras-induced cellular senescence (via p53/p16Ink4a) and to DNA damage-induced ROS through an H2AX–NOXA1/14-3-3ζ regulatory mechanism, expanding NOX1's role beyond proliferation to include growth-arrest and DNA-damage responses.

    Evidence Nox1 KO MEFs and siRNA blocking Ras-induced senescence markers; co-IP showing H2AX-dependent release of NOXA1 from 14-3-3ζ inhibition

    PMID:22237206 PMID:23216904

    Open questions at the time
    • Whether senescence vs. transformation outcome depends on ROS threshold not established
    • NOXA1 phosphorylation site(s) controlling 14-3-3ζ binding not mapped
  10. 2013 High

    Intestinal epithelial-specific and pulmonary studies revealed NOX1 controls mucosal wound repair by oxidizing phosphatases PTEN/PTP-PEST to activate FAK/paxillin, and separately regulates pulmonary artery smooth muscle cell apoptosis through Kv1.5 channel stabilization, with transgene rescue confirming specificity.

    Evidence Intestinal epithelial-specific Nox1 KO with in vivo wound repair and phosphatase activity assays; Nox1 KO mice with transgene rescue, Kv1.5 protein and apoptosis measurements

    PMID:23241962 PMID:24233492

    Open questions at the time
    • How NOX1-derived ROS stabilize Kv1.5 protein is mechanistically unclear
    • Whether phosphatase oxidation is a universal NOX1 signaling mechanism across tissues untested
  11. 2014 High

    Identification of PKCβ1-mediated phosphorylation of NOX1 at Thr429 as required for NOXA1 docking provided the first direct post-translational regulatory mechanism on the NOX1 catalytic subunit itself.

    Evidence Mass spectrometry phosphosite identification; T429A mutagenesis; ITC measuring NOX1–NOXA1 binding affinity; PKCβ1 siRNA abolishing TNFα-induced ROS and VSMC migration

    PMID:25228390

    Open questions at the time
    • Whether additional phosphorylation sites exist on NOX1 not surveyed
    • Crystal/cryo-EM structure of the NOX1 complex unavailable
  12. 2015 High

    Patient-derived NOX1 missense variants (P330S, D360N) causing reduced ROS production and impaired epithelial defense against Campylobacter jejuni linked NOX1 loss-of-function to very-early-onset inflammatory bowel disease.

    Evidence Sanger sequencing of VEOIBD patients; ROS assay in model cells and murine crypts; pathogen infection assay

    PMID:26301257

    Open questions at the time
    • Penetrance and genetic modifiers in VEOIBD families not established
    • Whether NOX1 variants affect microbiome composition not examined
  13. 2016 High

    CRISPR knockout of p22phox confirmed its absolute requirement for NOX1 maturation and activity, Prdx6 was identified as a NOXA1 stabilizer whose dual enzymatic activities are needed for NOX1 activation, and NOX1 was shown to stabilize ADAM17 from ubiquitin-mediated degradation to promote EGFR-PI3K-AKT signaling.

    Evidence CRISPR KO of CYBA with reconstitution; yeast two-hybrid identifying Prdx6-NOXA1 interaction validated by co-IP and enzymatic mutants; co-IP of NOX1-ADAM17 with ubiquitination assay

    PMID:27094494 PMID:27614387 PMID:27874952

    Open questions at the time
    • How Prdx6 PLA2 activity mechanistically supports NOX1 activation is unclear
    • ADAM17 interaction awaits reciprocal validation
  14. 2017 High

    Redox proteomics identified NMDA receptor subunit NR1 oxidation at C744 as the molecular target by which NOX1-derived ROS suppress BDNF in cortical neurons and mediate depressive-like behavior, extending NOX1 function to CNS redox signaling.

    Evidence Nox1 KO mice; VTA-targeted miRNA knockdown; redox proteome analysis; NR1 C744A mutagenesis rescuing BDNF suppression

    PMID:28314819

    Open questions at the time
    • Whether NOX1 oxidizes other neuronal substrates not surveyed
    • Cell-type-specific NOX1 expression in brain not fully mapped
  15. 2021 High

    NOX1 was established as a critical driver of stem cell self-renewal: colonic stem cell proliferation depends on TLR-activated NOX1–EGFR signaling, and spermatogonial stem cells require NOX1-derived ROS for a BCL6B–NOX1 feed-forward loop under normoxia and HIF1A regulation under hypoxia.

    Evidence NOX1 KO colonoids and mice with single-cell ROS and EGFR readouts; Nox1 KO SSC cultures under normoxia/hypoxia with BCL6B/HIF1A/CDKN1A epistasis

    PMID:33446567 PMID:33826887

    Open questions at the time
    • Whether NOX1 governs stem cell fate in other tissues unknown
    • Direct ROS targets in colonic stem cells not identified
  16. 2022 High

    Renal NOXA1-NOX1 activity was shown to be required for aldosterone/Ang II-induced ENaC activation and sodium retention, providing a molecular link between NOX1 and electrolyte homeostasis in collecting duct principal cells.

    Evidence Noxa1 KO mice with telemetric blood pressure, ENaC activity, and Na⁺ excretion; Noxa1 siRNA in renal epithelial cells

    PMID:34714114

    Open questions at the time
    • Whether NOX1 regulates other renal ion transporters not examined
    • Direct ROS target linking NOX1 to ENaC trafficking or gating undefined

Open questions

Synthesis pass · forward-looking unresolved questions
  • Despite extensive functional characterization, the atomic-resolution structure of the NOX1 holoenzyme complex has not been determined, the full repertoire of direct oxidation targets (beyond PTEN, PTP-PEST, NR1) is unknown, and the mechanisms deciding whether NOX1-derived ROS drive proliferation versus senescence versus necrosis in a given cellular context remain unresolved.
  • No cryo-EM or crystal structure of the NOX1 complex
  • Threshold or compartmentalization rules governing divergent cell-fate outcomes not defined
  • Relative contributions of NOXO1 vs. Tks4/Tks5 organizers in vivo not genetically dissected

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016491 oxidoreductase activity 6 GO:0140657 ATP-dependent activity 3
Localization
GO:0005886 plasma membrane 2 GO:0005794 Golgi apparatus 1
Pathway
R-HSA-162582 Signal Transduction 5 R-HSA-1430728 Metabolism 3 R-HSA-168256 Immune System 2 R-HSA-5357801 Programmed Cell Death 2
Partners
ARHGEF7CYBANOXA1NOXO1PRDX6RAC1SH3PXD2ASH3PXD2B
Complex memberships
NOX1-p22phox-NOXO1-NOXA1-Rac1 complexTRADD-RIP1-NOX1-Rac1 necrosis complex

Evidence

Reading pass · 40 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1999 NOX1 (originally Mox1) encodes a homologue of the catalytic subunit gp91phox of the phagocyte NADPH oxidase and generates superoxide in non-phagocytic cells; overexpression in NIH3T3 cells increases superoxide production, cell growth rate, anchorage-independent growth, and tumor formation in athymic mice, while antisense NOX1 decreases superoxide generation and serum-stimulated growth in smooth muscle cells. Overexpression and antisense inhibition in NIH3T3 and vascular smooth muscle cells; superoxide measurements; tumor formation assay in athymic mice Nature High 10485709
2001 H2O2 (derived from NOX1-generated superoxide via dismutation) acts as the key intracellular signal mediating NOX1-driven cell growth and transformation; co-expression of catalase with NOX1 reverses the transformed appearance, normalizes growth rate, prevents tumor formation, and restores >60% of NOX1-regulated genes to normal levels. Co-expression of human catalase with NOX1 in NIH3T3 cells; H2O2 and O2- measurements; gene expression profiling; tumor assays in athymic mice Proceedings of the National Academy of Sciences of the United States of America High 11331784
2001 NOX1 mediates the early phase of angiotensin II- and PDGF-induced superoxide production in vascular smooth muscle cells and is required for activation of the redox-sensitive signaling molecules p38 MAPK and Akt by Ang II; antisense NOX1 adenovirus completely inhibited this early superoxide phase and downstream redox signaling without affecting redox-independent pathways. Antisense adenoviral knockdown of NOX1 in VSMCs; superoxide measurement; Western blot for p38 MAPK and Akt phosphorylation Circulation research High 11348997
2002 NOX1 requires cytosolic activator proteins to generate superoxide; two novel colon-enriched proteins NOXO1 (p47phox homologue, lacks autoinhibitory domain and PKC phosphorylation sites) and NOXA1 (p67phox homologue, lacks first SH3 domain, has hydrophobic stretch) were identified; co-expression of NOX1 with NOXO1 and NOXA1 leads to constitutive high-level superoxide generation, whereas replacing NOXO1 with p47phox restores stimulus-dependence. Co-expression of NOX1 with p47phox/p67phox or novel NOXO1/NOXA1 in heterologous cells; superoxide measurements; cDNA cloning The Journal of biological chemistry High 12473664
2002 NOX1-derived ROS trigger the angiogenic switch by markedly upregulating VEGF mRNA (in cultured cells and tumors) and inducing VEGFR1/VEGFR2 in vascular cells; matrix metalloproteinase activity is also induced; VEGF induction is abolished by catalase co-expression, implicating H2O2 as the signaling intermediate. NOX1 overexpression in DU-145 prostate cells; VEGF mRNA measurement; immunostaining of VEGF receptors in tumors; catalase co-expression; MMP activity assay Proceedings of the National Academy of Sciences of the United States of America High 11805326
2004 Oncogenic Ras upregulates NOX1 expression via the MEK-MAPK pathway, and NOX1-generated ROS are required for Ras-induced transformation; siRNA knockdown of NOX1 blocks anchorage-independent growth, morphological transformation, and tumor formation in athymic mice caused by activated Ras. siRNA knockdown of NOX1 in Ras-transformed cells; MEK inhibitor; anchorage-independent growth assay; tumor formation in athymic mice Cancer research High 15150115
2004 NOX1 activity in growth factor-stimulated cells is mediated by sequential activation of PI3K → βPix (a Rac GEF that constitutively associates with the C-terminal region of NOX1) → Rac1 (which binds to the C-terminal region of NOX1 in a growth factor-dependent manner); siRNA depletion of βPix blocks both Rac1 activation and ROS production. Co-immunoprecipitation of NOX1 C-terminal region with βPix and Rac1; siRNA knockdown of βPix and Nox1; ROS measurement; PI3K and other pathway inhibitors Molecular and cellular biology High 15121857
2004 NOX1 physically associates with p22phox in vascular smooth muscle cells; co-immunoprecipitation and confocal colocalization demonstrate the two proteins are preassembled at the plasma membrane and submembrane areas in unstimulated cells, and NADPH-driven superoxide production depends on coexpression of both subunits. Co-immunoprecipitation of HA-tagged NOX1 with p22phox; confocal colocalization; ESR spin-probe measurement of NADPH-driven superoxide in cells expressing both subunits Free radical biology & medicine High 15477006
2005 NOX1 is required for angiotensin II-induced superoxide production and the resultant pressor response in vivo; Nox1-knockout mice show significantly blunted blood pressure elevation and aortic superoxide production in response to Ang II, with preserved endothelium-dependent relaxation and cGMP levels, indicating NOX1-derived ROS reduce NO bioavailability to mediate hypertension. Nox1 knockout mice; Ang II infusion; telemetric blood pressure measurement; superoxide production in aorta; endothelium-dependent relaxation; cGMP measurement; L-NAME experiments Circulation High 16246966
2007 TNF treatment induces formation of a signaling complex containing TRADD, RIP1, NOX1, and the small GTPase Rac1 in mouse fibroblasts undergoing necrosis; RIP1 is essential for NOX1 recruitment to this complex; dominant-negative TRADD or Rac1 and NOX1 siRNA each prevent TNF-induced superoxide generation and necrotic cell death. Co-immunoprecipitation demonstrating TRADD/RIP1/NOX1/Rac1 complex; RIP1-deficient fibroblasts; dominant-negative mutants; NOX1 siRNA; superoxide measurement; cell death assay Molecular cell High 17560373
2009 The C-terminal cytosolic tail of NOX1 confers cytosolic subunit-dependent activation, while the N-terminal transmembrane portion determines plasma membrane localization and superoxide (vs. H2O2) release; Nox1 N-terminus is proteolytically cleaved; chimeric NOX1/NOX4 proteins reveal that a Nox4 cytosolic tail fused to Nox1 transmembrane domain renders NOX1 constitutively active. Chimeric NOX1/NOX4 constructs expressed in HEK293 cells; TIRF microscopy for subcellular localization; Myc-tag processing analysis; ROS measurements Antioxidants & redox signaling High 19061439
2009 Tks4 and Tks5 (c-Src substrate proteins) are functional p47phox-related organizers that selectively support NOX1 (and NOX3) activity; Tks proteins interact with the NOXA1 activator through an SH3 domain-mediated interaction; endogenous Tks4 is required for Rac GTPase- and NOX1-dependent ROS production and for NOX1 recruitment to invadopodia in DLD1 colon cancer cells. Reconstituted cellular systems; co-immunoprecipitation (Tks-NoxA1 SH3 interaction); siRNA knockdown of Tks4; ROS measurement; invadopodia formation assay Science signaling High 19755710
2010 LRRC8A (a component of volume-regulated anion channels) co-localizes with and co-immunoprecipitates with NOX1 and its p22phox subunit in vascular smooth muscle cells; LRRC8A siRNA suppresses TNFα-induced extracellular superoxide production by NOX1, TNFR1 endocytosis, and JNK phosphorylation; extracellular superoxide (not H2O2) is the critical oxidant enabling TNFR1 endocytosis. Co-immunoprecipitation; siRNA knockdown; VRAC inhibitor; extracellular SOD/catalase discrimination; TNFR1 endocytosis assay; JNK phosphorylation Free radical biology & medicine High 27838438
2010 TNFα-induced NOX1 activation in smooth muscle cells requires dynamin-dependent receptor endocytosis, whereas thrombin-mediated NOX1 activation occurs outside endosomes and requires EGFR transactivation; both pathways converge on PI3K-Akt-ATF-1 signaling in a NOX1- and dynamin-dependent manner. NOX1 shRNA; dominant-negative dynamin; endosomal ROS measurement; EGFR transactivation assay; PI3K-Akt-ATF-1 pathway analysis Antioxidants & redox signaling High 19737091
2010 NOX1 upregulates EGFR and TGF-α expression through an Src-ERK positive feedback loop and a parallel p38 MAPK-AKT pathway, driving autocrine growth of liver tumor cells; NOX1 knockdown reduces EGFR/TGF-α mRNA and EGFR phosphorylation, and antioxidants or NADPH oxidase inhibition attenuates autocrine growth. NOX1 siRNA; antioxidants; pharmacological NADPH oxidase inhibition; EGFR/TGF-α mRNA and protein measurement; EGFR phosphorylation; pathway inhibitors The Journal of biological chemistry High 20525691
2010 NOX1-derived ROS promote invadopodia formation (extracellular matrix-degrading actin-rich structures) in human colon cancer cells; the selective NOX1 small-molecule inhibitor ML171 (2-acetylphenothiazine) blocks NOX1-dependent ROS generation and invadopodia formation at nanomolar concentrations with selectivity over other NOX isoforms; NOX1 overexpression rescues ML171 inhibition, confirming selectivity. High-throughput screening; ML171 pharmacological inhibition; NOX1 overexpression rescue; invadopodia assay; isoform selectivity panel ACS chemical biology High 20715845
2011 Annexin A1 (ANXA1) activates epithelial NOX1 through formyl peptide receptor 1 (FPR1) signaling; NOX1-derived ROS oxidatively inactivate phosphatases PTEN and PTP-PEST, leading to activation of FAK and paxillin, thereby promoting epithelial cell migration and mucosal wound repair; intestinal epithelial-specific Nox1 knockout mice show defects in mucosal wound repair. Intestinal epithelial-specific Nox1 knockout mice; ANXA1 administration; phosphatase activity (PTEN, PTP-PEST); FAK and paxillin activation; cell migration assay; in vivo wound repair The Journal of clinical investigation High 23241962
2012 DNA damage-induced ROS generation is mediated through the H2AX-NOX1/Rac1 pathway; H2AX overexpression or NCS-induced DNA damage increases NOX1 activity partly by reducing the interaction between the NOX1 activator NOXA1 and its inhibitor 14-3-3ζ; NOX1 knockdown (but not NOX4) reduces ROS and cell death induced by H2AX accumulation. siRNA knockdown of NOX1 and NOX4; dominant-negative Rac1; NADPH oxidase inhibitor DPI; ROS measurement; co-immunoprecipitation (NOXA1/14-3-3ζ interaction); cell death assay Cell death & disease High 22237206
2012 In rat REF52 cells, oncogenic Ras upregulates NOX1 through the Ras/MEK pathway to increase intracellular ROS, which transduce senescence signals activating the p53 and p16Ink4a pathways; NOX1 siRNA blocks Ras-induced senescence (β-galactosidase activity, growth arrest), DNA damage response, and p38MAPK activation; this is confirmed in Nox1 knockout mouse embryo fibroblasts. siRNA knockdown of NOX1; Nox1 knockout MEFs; senescence markers (β-gal, p53, p16); DNA damage assay; p38 MAPK phosphorylation; MEK inhibitor Genes to cells : devoted to molecular & cellular mechanisms High 23216904
2014 Protein kinase C-β1 phosphorylates NOX1 at threonine 429 in response to TNFα; this phosphorylation facilitates association of NOX1 with the NoxA1 activation domain, is necessary for NADPH oxidase complex assembly, ROS production, and vascular smooth muscle cell migration; PKCβ1 siRNA abolishes TNFα-mediated ROS production and VSMC migration. Mass spectrometry identifying phosphorylation site; PKCβ1 inhibitor and siRNA; site-directed mutagenesis (T429A); isothermal titration calorimetry (NOX1/NoxA1 interaction); ROS measurement; VSMC migration assay Circulation research High 25228390
2016 Peroxiredoxin 6 (Prdx6) is a novel binding partner of NOXA1, identified by yeast two-hybrid using the NOXA1 SH3 domain as bait; Prdx6 binds to and stabilizes NOXA1 via SH3 domain interaction; both the peroxidase (C47S) and phospholipase A2 (S32A) activities of Prdx6 are required for NOX1 activation and NOX1-mediated cell migration; the PLA2 transition-state inhibitor MJ-33 suppresses NOX1 activity. Yeast two-hybrid; co-immunoprecipitation; Prdx6 knockdown and overexpression; enzymatic mutants (C47S, S32A); MJ-33 inhibitor; superoxide measurement; cell migration assay Free radical biology & medicine High 27094494
2016 CRISPR/Cas9-mediated knockout of p22phox (CYBA) abolishes NOX1 (and NOX4) activity but not NOX5 activity; p22phox is required for maturation and activity of NOX1; rescue with human or rat p22phox, but not DUOXA1/A2, restores NOX1-dependent superoxide production. CRISPR/Cas9 knockout; reconstitution with human/rat p22phox and DUOXA1/A2; ROS measurements (H2O2 and superoxide); multiple p22phox mutation analysis Redox biology High 27614387
2017 NOX1-derived ROS in the ventral tegmental area (VTA) mediate depressive-like behaviors by oxidizing NMDA receptor subunit NR1 (at C744); NOX1-derived ROS suppress NMDA-induced BDNF upregulation in cortical neurons via NR1 oxidation; NR1 C744A mutant is resistant to H2O2-mediated suppression of BDNF; redox proteomics identified NR1 as the NOX1 target. Nox1 knockout mice; miRNA delivery to VTA; chronic social defeat stress and corticosterone models; redox proteome analysis; cortical neuron culture with H2O2 and NR1 C744A mutant; BDNF mRNA and DNA methylation measurements The Journal of neuroscience High 28314819
2013 NOX1 is required for normal apoptosis of pulmonary arterial smooth muscle cells (PASMCs) through regulation of the voltage-gated K+ channel Kv1.5 and intracellular potassium levels; Nox1-deficient mice develop spontaneous pulmonary vascular remodeling with reduced PASMC apoptosis and decreased Kv1.5 protein; re-expression of rat NOX1 transgene in knockout mice rescues impaired apoptosis and Kv1.5 levels. Nox1 knockout mice; Nox1 transgene rescue; flow cytometry and TUNEL for apoptosis; Kv1.5 protein measurement; intracellular potassium measurement Arteriosclerosis, thrombosis, and vascular biology High 24233492
2011 NOX1 mediates morphine-induced analgesic tolerance by attenuating opioid pharmacology; Nox1-deficient mice show augmented morphine analgesia and suppressed tolerance; NOX1-derived ROS regulate GTPase activity in the dorsal spinal cord, impair G-protein coupling, promote PKC isoform membrane translocation, enhance RGS9-2 phosphorylation, and facilitate Gαi2/RGS9-2/14-3-3 complex formation. Nox1 knockout mice; [35S]GTPγS binding; morphine-stimulated GTPase activity; PKC membrane translocation assay; RGS9-2 phosphorylation; co-immunoprecipitation (Gαi2/RGS9-2/14-3-3) The Journal of neuroscience High 22159121
2007 STAT1 and STAT3 directly bind to GAS elements in the NOX1 promoter (demonstrated by chromatin immunoprecipitation) and transactivate NOX1 expression in human vascular smooth muscle cells; JAK/STAT pathway regulates IFNγ-induced NOX1 activity; JAK/STAT blockade reduces NOX1 subunit transcription. Chromatin immunoprecipitation; promoter-luciferase assays; STAT1/STAT3 overexpression; JAK/STAT inhibitors; NOX activity measurement Arteriosclerosis, thrombosis, and vascular biology High 19834108
2007 MEF2B transcription factor binds to a consensus MEF2 site in the NOX1 promoter (-146 to -125) in response to prostaglandin F2α; MEF2B is downstream of ATF-1 in a PGF2α/PDGF-induced cascade (ATF-1→MEF2B→NOX1); MEF2B siRNA suppresses NOX1 expression and reduces VSMC superoxide production. Promoter deletion analysis; MEF2 site binding; MEF2B siRNA; ATF-1 siRNA; RT-PCR of NOX1; superoxide assay in VSMCs The FEBS journal High 17822438
2007 Nox1, together with its partner proteins NOXO1 and NOXA1, is localized predominantly to the Golgi apparatus in human gastric adenocarcinoma cells (intestinal-type, diffuse-type, and signet-ring cell carcinoma), with nuclear localization also observed in diffuse-type; co-expression of Nox1, NOXO1, NOXA1, and p22phox is specific to gastric cancer cells and absent from normal gastric mucosa. Immunohistochemistry; confocal microscopy showing subcellular localization; immunofluorescence Free radical biology & medicine Medium 18037128
2007 NOX1 overexpression induces genome instability; co-expression of NOX1 with NOXO1 and NOXA1 in HeLa cells increases 8-oxoguanine levels and causes a 3-fold increase in HPRT mutation rate, without upregulating DNA repair enzymes, suggesting ROS-mediated saturation of repair capacity. Co-expression of hNOX1/NOXO1/NOXA1 in HeLa cells; ROS measurement; 8-oxoguanine quantification; HPRT mutation rate; repair enzyme expression analysis Free radical biology & medicine Medium 17963706
2010 NOX1 inhibits p53 proapoptotic transcriptional activity and p53 Lys382 acetylation through a SIRT1-dependent mechanism; NOX1 activates SIRT1 deacetylase activity, and SIRT1 deacetylase-defective mutant (SIRT1HY) renders cells unresponsive to NOX1-dependent inhibition of p53; recovery of HIPK2 function downregulates NOX1 and rescues p53 Lys382 acetylation. siRNA knockdown of NOX1 and SIRT1; SIRT1 inhibitor nicotinamide; SIRT1HY deacetylase-dead mutant; p53 Lys382 acetylation and transcriptional activity assays; HIPK2 rescue experiment Free radical biology & medicine Medium 20171273
2014 NOX1 is the key NADPH oxidase regulating GPVI-dependent ROS production in platelets; pharmacological inhibition of NOX1 with ML171 blocks GPVI (collagen-related peptide)-induced ROS, thromboxane A2 production (via p38 MAPK signaling), and reduces collagen-mediated thrombus formation at arterial shear in ex vivo perfusion; Nox2-deficient mice confirm NOX1 specificity for this pathway. ML171 pharmacological inhibition; Nox2-deficient mice; p38 MAPK inhibition; TxA2 measurement; platelet aggregation assay; ex vivo perfusion thrombus model Redox biology Medium 24494191
2016 NOX1 directly interacts with and stabilizes ADAM17 (TACE) from ubiquitin-mediated degradation; NOX1 overexpression activates the ADAM17-EGFR-PI3K-AKT signaling pathway to promote colon cancer cell migration and invasion; co-immunoprecipitation demonstrates the NOX1-ADAM17 physical interaction. Co-immunoprecipitation; NOX1 overexpression; ADAM17 ubiquitination assay; EGFR-PI3K-AKT pathway Western blot; migration/invasion assay European review for medical and pharmacological sciences Medium 27874952
2021 NOX1-derived ROS mediate colonic stem cell (CSC) proliferation through redox-dependent activation of EGFR signaling; NOX1 expression is CSC-specific and restricted to proliferating CSCs; in the absence of NOX1, CSCs fail to generate ROS and have reduced proliferation; NOX1 expression is regulated by Toll-like receptor activation in response to the microbiota, establishing a TLR-NOX1-EGFR axis. NOX1 knockout colonoids and mice; single-cell ROS measurement; EGFR signaling readout; TLR stimulation; CSC-specific NOX1 expression analysis Cell reports High 33826887
2022 Renal NOXA1-dependent NOX1 activity is required for Ang II-induced upregulation of epithelial sodium channel (ENaC) in collecting duct principal cells and sodium retention; Noxa1 knockout mice show attenuated Ang II-induced hypertension and impaired ENaC activation; aldosterone induces ROS and Noxa1/Scnn1a expression and ENaC activity through NOXA1-NOX1, abolished by Noxa1 siRNA. Noxa1 knockout mice; telemetric blood pressure; ENaC activity measurement; Na+ excretion assay; Noxa1 siRNA in renal epithelial cells; ROS measurement Antioxidants & redox signaling High 34714114
2021 NOX1-derived ROS drive spermatogonial stem cell (SSC) self-renewal under normoxia through a ROS-BCL6B-NOX1 feed-forward pathway; under hypoxia, NOX1-derived (not mitochondrial) ROS influence HIF1A expression in undifferentiated spermatogonia; Nox1-deficient SSCs proliferate poorly under hypoxia but normally under normoxia; CDKN1A depletion rescues impaired Nox1-deficient SSC proliferation under hypoxia. Nox1 knockout mice and SSC cultures; normoxia/hypoxia conditions; BCL6B expression; HIF1A measurement; CDKN1A siRNA rescue; mitochondrial ROS suppression Genes & development High 33446567
2019 NF-κB directly regulates NOXO1 expression in TNF-α-stimulated gastric cancer cells, activating the NOX1 complex; NOX1/ROS signaling increases proliferation of gastric epithelial cells and expands SOX2-positive undifferentiated epithelial stem cells; disruption of Noxo1 in gastritis mouse model suppresses metaplastic hyperplasia and reduces SOX2-positive cells. NF-κB inhibition and overexpression; NOXO1 promoter analysis; Noxo1 knockout in K19-C2mE mice; in situ hybridization; SOX2 immunostaining; gastric hyperplasia histology Oncogene High 30700829
2004 Helicobacter pylori LPS activates NOX1 in gastric mucosal cells through two distinct events: transcriptional upregulation of Nox1 and NOXO1 mRNAs, and activation of Rac1 (GTP-bound state) via PI3K; a constitutively active Rac1 adenovirus (but not Cdc42) restores PI3K inhibitor-blocked superoxide generation, establishing Rac1 as an essential NOX1 activator. Primary guinea pig gastric mucosal cells; Rac1 GTP-loading assay; adenoviral constitutively active Rac1/Cdc42; PI3K inhibitor LY294002; actinomycin D and cycloheximide blocking; superoxide measurement American journal of physiology. Cell physiology High 15469954
2015 NOX1 missense variants p.Pro330Ser and p.Asp360Asn found in VEOIBD patients show reduced ROS production compared to wild-type NOX1 despite appropriate cellular localization; cells harboring these variants have defective host resistance to Campylobacter jejuni infection, establishing that NOX1 ROS generation is required for epithelial innate defense. Sanger sequencing; ROS generation assay in model cell lines and murine crypts; cellular localization assessment; pathogen infection assay (C. jejuni, EPEC); structural homology modeling Cellular and molecular gastroenterology and hepatology High 26301257
2007 The NOX1 promoter region contains a GATA-4/5/6 binding element and overlapping sites for Cdx1/Cdx2 and HNF-1α; these factors show cooperativity in transactivating the NOX1 promoter; GATA-6, HNF-1α, and Cdx2 bind to this chromatin region in CaCo2 cells; their expression gradients in mouse colon parallel the distal-to-proximal NOX1 gradient. Promoter-luciferase deletion analysis; in vitro DNA binding; chromatin immunoprecipitation in CaCo2 cells; transcription factor co-transfection cooperativity assay; NOX1 expression gradient analysis in normal and germ-free mice Free radical biology & medicine High 18005670
2011 NOX1 is expressed in hepatic stellate cells (HSCs) but not Kupffer cells; NOX1 mediates profibrogenic effects in endogenous liver cells; NOX1-deficient HSCs show decreased ROS generation and fail to upregulate collagen α1(I) and TGF-β in response to angiotensin II; bone marrow chimera experiments confirm that NOX1 acts in endogenous liver cells rather than bone marrow-derived cells. Nox1 knockout mice; CCl4 and bile duct ligation hepatic fibrosis models; bone marrow chimeras; HSC and Kupffer cell isolation; ROS generation; collagen/TGF-β measurement Hepatology (Baltimore, Md.) High 21384410

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1999 Cell transformation by the superoxide-generating oxidase Mox1. Nature 1215 10485709
2001 Novel gp91(phox) homologues in vascular smooth muscle cells : nox1 mediates angiotensin II-induced superoxide formation and redox-sensitive signaling pathways. Circulation research 720 11348997
2007 TNF-induced activation of the Nox1 NADPH oxidase and its role in the induction of necrotic cell death. Molecular cell 429 17560373
2005 Nox1 is involved in angiotensin II-mediated hypertension: a study in Nox1-deficient mice. Circulation 414 16246966
2002 Two novel proteins activate superoxide generation by the NADPH oxidase NOX1. The Journal of biological chemistry 406 12473664
2002 Reactive oxygen generated by Nox1 triggers the angiogenic switch. Proceedings of the National Academy of Sciences of the United States of America 397 11805326
2001 Hydrogen peroxide mediates the cell growth and transformation caused by the mitogenic oxidase Nox1. Proceedings of the National Academy of Sciences of the United States of America 396 11331784
2004 The superoxide-generating oxidase Nox1 is functionally required for Ras oncogene transformation. Cancer research 266 15150115
2005 Increased Nox1 and hydrogen peroxide in prostate cancer. The Prostate 249 15389790
2012 DNA damage induces reactive oxygen species generation through the H2AX-Nox1/Rac1 pathway. Cell death & disease 248 22237206
2012 Annexin A1, formyl peptide receptor, and NOX1 orchestrate epithelial repair. The Journal of clinical investigation 247 23241962
2000 A mammalian H+ channel generated through alternative splicing of the NADPH oxidase homolog NOH-1. Science (New York, N.Y.) 238 10615049
2001 Upregulation of the vascular NAD(P)H-oxidase isoforms Nox1 and Nox4 by the renin-angiotensin system in vitro and in vivo. Free radical biology & medicine 228 11728818
2004 Sequential activation of phosphatidylinositol 3-kinase, beta Pix, Rac1, and Nox1 in growth factor-induced production of H2O2. Molecular and cellular biology 201 15121857
2011 The nicotinamide adenine dinucleotide phosphate oxidase (NOX) homologues NOX1 and NOX2/gp91(phox) mediate hepatic fibrosis in mice. Hepatology (Baltimore, Md.) 171 21384410
2005 Expression of NOX1, a superoxide-generating NADPH oxidase, in colon cancer and inflammatory bowel disease. The Journal of pathology 169 16086438
2010 A novel and specific NADPH oxidase-1 (Nox1) small-molecule inhibitor blocks the formation of functional invadopodia in human colon cancer cells. ACS chemical biology 158 20715845
2009 Roles of Nox1 and other Nox isoforms in cancer development. Cancer science 148 19493276
2008 Nox1 is over-expressed in human colon cancers and correlates with activating mutations in K-Ras. International journal of cancer 133 18398843
2021 Glyphosate damages blood-testis barrier via NOX1-triggered oxidative stress in rats: Long-term exposure as a potential risk for male reproductive health. Environment international 128 34906888
2010 HIF-1 alpha signaling is augmented during intermittent hypoxia by induction of the Nrf2 pathway in NOX1-expressing adenocarcinoma A549 cells. Free radical biology & medicine 126 20347035
2009 Identification of structural elements in Nox1 and Nox4 controlling localization and activity. Antioxidants & redox signaling 125 19061439
2009 Jak/STAT signaling pathway regulates nox1 and nox4-based NADPH oxidase in human aortic smooth muscle cells. Arteriosclerosis, thrombosis, and vascular biology 124 19834108
2015 Defects in NADPH Oxidase Genes NOX1 and DUOX2 in Very Early Onset Inflammatory Bowel Disease. Cellular and molecular gastroenterology and hepatology 118 26301257
2004 Helicobacter pylori lipopolysaccharide activates Rac1 and transcription of NADPH oxidase Nox1 and its organizer NOXO1 in guinea pig gastric mucosal cells. American journal of physiology. Cell physiology 117 15469954
2005 Differential effects of diabetes on the expression of the gp91phox homologues nox1 and nox4. Free radical biology & medicine 109 15993337
2019 Differential contribution of Nox1, Nox2 and Nox4 to kidney vascular oxidative stress and endothelial dysfunction in obesity. Redox biology 104 31563085
2012 Integration of pro-inflammatory cytokines, 12-lipoxygenase and NOX-1 in pancreatic islet beta cell dysfunction. Molecular and cellular endocrinology 100 22502743
2011 NOX1, 2, 4, 5: counting out oxidative stress. British journal of pharmacology 98 21323893
2005 Expression of NADPH oxidase isoform 1 (Nox1) in human placenta: involvement in preeclampsia. Placenta 98 15993942
2014 The role of Nox1 and Nox2 in GPVI-dependent platelet activation and thrombus formation. Redox biology 97 24494191
2003 Association of gp91phox homolog Nox1 with anchorage-independent growth and MAP kinase-activation of transformed human keratinocytes. Oncogene 94 12955083
2008 Exogenous hydrogen sulfide inhibits superoxide formation, NOX-1 expression and Rac1 activity in human vascular smooth muscle cells. Journal of vascular research 91 18463417
2012 ROS-generating oxidases Nox1 and Nox4 contribute to oncogenic Ras-induced premature senescence. Genes to cells : devoted to molecular & cellular mechanisms 89 23216904
2009 Novel p47(phox)-related organizers regulate localized NADPH oxidase 1 (Nox1) activity. Science signaling 86 19755710
2007 Nox1-dependent superoxide production controls colon adenocarcinoma cell migration. Biochimica et biophysica acta 82 18023288
2006 NADPH oxidases in the gastrointestinal tract: a potential role of Nox1 in innate immune response and carcinogenesis. Antioxidants & redox signaling 81 16987012
2017 Zinc regulates Nox1 expression through a NF-κB and mitochondrial ROS dependent mechanism to induce senescence of vascular smooth muscle cells. Free radical biology & medicine 77 28363602
2011 Differential regulation of Nox1, Nox2 and Nox4 in vascular smooth muscle cells from WKY and SHR. Journal of the American Society of Hypertension : JASH 74 21419746
2015 Mouse lung development and NOX1 induction during hyperoxia are developmentally regulated and mitochondrial ROS dependent. American journal of physiology. Lung cellular and molecular physiology 69 26092998
2015 NOX1 to NOX2 switch deactivates AMPK and induces invasive phenotype in colon cancer cells through overexpression of MMP-7. Molecular cancer 69 26116564
2010 A differential role for endocytosis in receptor-mediated activation of Nox1. Antioxidants & redox signaling 69 19737091
2007 Evidence for cancer-associated expression of NADPH oxidase 1 (Nox1)-based oxidase system in the human stomach. Free radical biology & medicine 68 18037128
2004 Functional association of nox1 with p22phox in vascular smooth muscle cells. Free radical biology & medicine 65 15477006
2020 The TSPO-NOX1 axis controls phagocyte-triggered pathological angiogenesis in the eye. Nature communications 63 32483169
2010 Nox1 is involved in p53 deacetylation and suppression of its transcriptional activity and apoptosis. Free radical biology & medicine 63 20171273
2019 NF-κB-induced NOX1 activation promotes gastric tumorigenesis through the expansion of SOX2-positive epithelial cells. Oncogene 60 30700829
2015 The Nox1/4 Dual Inhibitor GKT137831 or Nox4 Knockdown Inhibits Angiotensin-II-Induced Adult Mouse Cardiac Fibroblast Proliferation and Migration. AT1 Physically Associates With Nox4. Journal of cellular physiology 60 26445208
2010 NADPH oxidase NOX1 controls autocrine growth of liver tumor cells through up-regulation of the epidermal growth factor receptor pathway. The Journal of biological chemistry 60 20525691
2018 NOX1-derived ROS drive the expression of Lipocalin-2 in colonic epithelial cells in inflammatory conditions. Mucosal immunology 59 30279516
2016 Nox1 in cardiovascular diseases: regulation and pathophysiology. Clinical science (London, England : 1979) 58 26678171
2022 Osteopontin promotes hepatocellular carcinoma progression through inducing JAK2/STAT3/NOX1-mediated ROS production. Cell death & disease 57 35418176
2017 Depressive-Like Behaviors Are Regulated by NOX1/NADPH Oxidase by Redox Modification of NMDA Receptor 1. The Journal of neuroscience : the official journal of the Society for Neuroscience 56 28314819
2019 SHMT1 inhibits the metastasis of HCC by repressing NOX1-mediated ROS production. Journal of experimental & clinical cancer research : CR 55 30755243
2004 Nox1 regulates apoptosis and potentially stimulates branching morphogenesis in sinusoidal endothelial cells. Experimental cell research 53 15475009
2011 Involvement of NOX1/NADPH oxidase in morphine-induced analgesia and tolerance. The Journal of neuroscience : the official journal of the Society for Neuroscience 49 22159121
2008 Nox1 downstream of 12-lipoxygenase controls cell proliferation but not cell spreading of colon cancer cells. International journal of cancer 49 18076063
2021 NOX1-dependent redox signaling potentiates colonic stem cell proliferation to adapt to the intestinal microbiota by linking EGFR and TLR activation. Cell reports 48 33826887
2016 Peroxiredoxin 6 (Prdx6) supports NADPH oxidase1 (Nox1)-based superoxide generation and cell migration. Free radical biology & medicine 48 27094494
2015 Unchanged NADPH Oxidase Activity in Nox1-Nox2-Nox4 Triple Knockout Mice: What Do NADPH-Stimulated Chemiluminescence Assays Really Detect? Antioxidants & redox signaling 47 25906178
1994 Isolation of a diverged homeobox gene, MOX1, from the BRCA1 region on 17q21 by solution hybrid capture. Human molecular genetics 47 7987315
2023 Rhein Inhibited Ferroptosis and EMT to Attenuate Diabetic Nephropathy by Regulating the Rac1/NOX1/β-Catenin Axis. Frontiers in bioscience (Landmark edition) 46 37258467
2022 NOX1 is essential for TNFα-induced intestinal epithelial ROS secretion and inhibits M cell signatures. Gut 46 36191961
2016 LRRC8A channels support TNFα-induced superoxide production by Nox1 which is required for receptor endocytosis. Free radical biology & medicine 43 27838438
2007 Overexpression of human NOX1 complex induces genome instability in mammalian cells. Free radical biology & medicine 43 17963706
2017 Vascular dysfunction and fibrosis in stroke-prone spontaneously hypertensive rats: The aldosterone-mineralocorticoid receptor-Nox1 axis. Life sciences 42 28478264
2014 The NOX1/4 inhibitor GKT136901 as selective and direct scavenger of peroxynitrite. Current medicinal chemistry 40 23848532
1996 Differential localization of Mox-1 and Mox-2 proteins indicates distinct roles during development. The International journal of developmental biology 40 9032023
2016 CRISPR/Cas9-mediated knockout of p22phox leads to loss of Nox1 and Nox4, but not Nox5 activity. Redox biology 38 27614387
2006 NADPH oxidase subunits (NOX-1, p22phox, Rac-1) and tacrolimus-induced nephrotoxicity in a rat renal transplant model. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 38 17085464
2019 Leptin Restores Endothelial Function via Endothelial PPARγ-Nox1-Mediated Mechanisms in a Mouse Model of Congenital Generalized Lipodystrophy. Hypertension (Dallas, Tex. : 1979) 37 31656096
2016 Effects of NOX1 on fibroblastic changes of endothelial cells in radiation‑induced pulmonary fibrosis. Molecular medicine reports 37 27053172
2022 Dioscin alleviates myocardial infarction injury via regulating BMP4/NOX1-mediated oxidative stress and inflammation. Phytomedicine : international journal of phytotherapy and phytopharmacology 36 35675750
2016 The ROS-generating oxidase Nox1 is required for epithelial restitution following colitis. Experimental animals 35 26876598
2021 An interplay of NOX1-derived ROS and oxygen determines the spermatogonial stem cell self-renewal efficiency under hypoxia. Genes & development 34 33446567
2014 Matrix metalloproteinase-3 causes dopaminergic neuronal death through Nox1-regenerated oxidative stress. PloS one 34 25536219
2001 Homeodomain proteins Mox1 and Mox2 associate with Pax1 and Pax3 transcription factors. FEBS letters 34 11423130
2016 Internal Pudental Artery Dysfunction in Diabetes Mellitus Is Mediated by NOX1-Derived ROS-, Nrf2-, and Rho Kinase-Dependent Mechanisms. Hypertension (Dallas, Tex. : 1979) 32 27528061
2013 Deficiency of NOX1/nicotinamide adenine dinucleotide phosphate, reduced form oxidase leads to pulmonary vascular remodeling. Arteriosclerosis, thrombosis, and vascular biology 32 24233492
2007 Regulation of NOX1 expression by GATA, HNF-1alpha, and Cdx transcription factors. Free radical biology & medicine 31 18005670
2016 Differential effects of NOX4 and NOX1 on immune cell-mediated inflammation in the aortic sinus of diabetic ApoE-/- mice. Clinical science (London, England : 1979) 30 27190136
2016 Nox1 promotes colon cancer cell metastasis via activation of the ADAM17 pathway. European review for medical and pharmacological sciences 30 27874952
2014 Phosphorylation of Nox1 regulates association with NoxA1 activation domain. Circulation research 30 25228390
2017 CD47 and Nox1 Mediate Dynamic Fluid-Phase Macropinocytosis of Native LDL. Antioxidants & redox signaling 28 27958762
2015 NOX1 mediates chemoresistance via HIF1α/MDR1 pathway in gallbladder cancer. Biochemical and biophysical research communications 28 26545779
2020 C1q/TNF-related protein 5 contributes to diabetic vascular endothelium dysfunction through promoting Nox-1 signaling. Redox biology 27 32122792
2010 Differential induction of reactive oxygen species through Erk1/2 and Nox-1 by FK228 for selective apoptosis of oncogenic H-Ras-expressing human urinary bladder cancer J82 cells. Journal of cancer research and clinical oncology 27 20473523
2022 Renal NOXA1/NOX1 Signaling Regulates Epithelial Sodium Channel and Sodium Retention in Angiotensin II-induced Hypertension. Antioxidants & redox signaling 25 34714114
2022 Setanaxib (GKT137831) Ameliorates Doxorubicin-Induced Cardiotoxicity by Inhibiting the NOX1/NOX4/Reactive Oxygen Species/MAPK Pathway. Frontiers in pharmacology 25 35444554
2021 Platelet-derived extracellular vesicles express NADPH oxidase-1 (Nox-1), generate superoxide and modulate platelet function. Free radical biology & medicine 25 33548451
2020 Nox1/4 inhibition exacerbates age dependent perivascular inflammation and fibrosis in a model of spontaneous hypertension. Pharmacological research 25 33131726
2019 The NADPH Oxidases Nox1 and Nox2 Differentially Regulate Volatile Organic Compounds, Fungistatic Activity, Plant Growth Promotion and Nutrient Assimilation in Trichoderma atroviride. Frontiers in microbiology 24 30728815
2015 Escherichia coli LF82 differentially regulates ROS production and mucin expression in intestinal epithelial T84 cells: implication of NOX1. Inflammatory bowel diseases 24 25822013
2010 Thyroid hormone induces artery smooth muscle cell proliferation: discovery of a new TRalpha1-Nox1 pathway. Journal of cellular and molecular medicine 24 20414976
2023 BRD9 Inhibition Attenuates Matrix Degradation and Pyroptosis in Nucleus Pulposus by Modulating the NOX1/ROS/NF-κB axis. Inflammation 23 36801999
2011 NOX1 participates in ROS-dependent cell death of colon epithelial Caco2 cells induced by Entamoeba histolytica. Microbes and infection 23 21723410
2007 Myocyte enhancer factor 2B is involved in the inducible expression of NOX1/NADPH oxidase, a vascular superoxide-producing enzyme. The FEBS journal 23 17822438
2019 ROS-generating oxidase NOX1 promotes the self-renewal activity of CD133+ thyroid cancer cells through activation of the Akt signaling. Cancer letters 22 30690057
2019 Nox1 downregulators: A new class of therapeutics. Steroids 22 31518594
2015 Protein disulfide isomerase expression increases in resistance arteries during hypertension development. Effects on Nox1 NADPH oxidase signaling. Frontiers in chemistry 22 25870854