Affinage

NOX1

NADPH oxidase 1 · UniProt Q9Y5S8

Length
564 aa
Mass
64.9 kDa
Annotated
2026-06-10
100 papers in source corpus 50 papers cited in narrative 50 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NOX1 (NADPH oxidase 1) is a plasma-membrane superoxide-generating enzyme that converts receptor and stress inputs into compartmentalized redox signals controlling proliferation, migration, senescence, angiogenesis, vascular tone and intestinal/neural homeostasis (PMID:11348997, PMID:11331784, PMID:18760347). Its N-terminal/transmembrane region targets it to the plasma membrane and dictates that it releases superoxide extracellularly—rapidly converted to H2O2—whereas swapping in Nox4 elements shifts it to intracellular vesicles and switches its output to H2O2 (PMID:18760347, PMID:19061439). NOX1 activity is built by an assembling multiprotein complex: the small GTPase Rac1 binds NOX1 directly and, with the organizer/activator subunits NOXO1 and NOXA1, is required for catalysis (PMID:16636067, PMID:15949904). Assembly is tuned post-translationally—PKCβ1 phosphorylates NOX1-Thr429 to license NOXA1 docking, PKA phosphorylation of NOXA1 (Ser172/Ser461) promotes 14-3-3 binding and complex disassembly, and PDI forms a redox-dependent disulfide with p47phox to drive assembly—and NOXA1 is further stabilized by Peroxiredoxin 6 (PMID:25228390, PMID:17913709, PMID:30580571, PMID:27094494). NOX1 is recruited to and activated by receptor/adaptor complexes including the AT1 receptor, TNF-induced TRADD/RIP1, TLR2, and CD47/thrombospondin-1, and it acts spatially through dynamin-dependent endosomal versus EGFR-transactivation routes (PMID:22636674, PMID:17560373, PMID:34127487, PMID:29042481, PMID:19737091). The resulting ROS execute signaling by oxidative inactivation of phosphatases (PTEN, PTP-PEST) and oxidation of redox-sensitive cysteines on Akt, the NMDA receptor subunit NR1, and S100A9 (activating mTORC1), thereby controlling integrin-dependent epithelial wound repair, cardiomyocyte apoptosis, cancer stem-cell proliferation, and depressive behavior (PMID:23241962, PMID:22982050, PMID:28314819, PMID:31365870). In vivo, NOX1 mediates the Ang II pressor response by limiting NO bioavailability, drives p53/p21-dependent and IL-6/SASP-linked vascular senescence, and shapes intestinal epithelial identity and inflammation, where loss-of-function NOX1 mutations impair colonic ROS production (PMID:16246966, PMID:29042481, PMID:34654740, PMID:36191961, PMID:29091079). Transcriptionally, NOX1 is induced by NF-κB, JAK/STAT (STAT1/STAT3 at GAS elements, antagonized by RGS2), gut-restricted GATA/HNF-1α/Cdx factors, and MEF2B (PMID:28363602, PMID:19834108, PMID:22120521, PMID:18005670, PMID:25550204).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 2001 High

    Established NOX1 as a functional superoxide-generating oxidase placed upstream of redox-sensitive kinase signaling and of oncogenic transformation, defining its core biological output.

    Evidence Antisense knockdown in VSMCs with superoxide and kinase readouts; catalase rescue in NOX1-transformed NIH 3T3 cells with tumor and gene-expression assays

    PMID:11331784 PMID:11348997

    Open questions at the time
    • Did not resolve whether the proximate signal is superoxide or its H2O2 product
    • Direct enzymatic mechanism not reconstituted
  2. 2002 Medium

    Identified H2O2 as the diffusible NOX1 messenger driving the angiogenic switch via VEGF/VEGFR induction.

    Evidence NOX1 overexpression with VEGF mRNA quantification and catalase co-expression rescue in vascular and tumor cells

    PMID:11805326

    Open questions at the time
    • Single-lab overexpression system
    • Direct transcriptional link to VEGF promoter not mapped
  3. 2008 High

    Resolved that NOX1 produces predominantly superoxide that is subsequently dismutated to H2O2, distinguishing it from Nox4.

    Evidence Isoform-selective depletion with ESR spin-trapping and Amplex Red in aortic SMCs

    PMID:18760347

    Open questions at the time
    • Did not address how ROS species selectivity is encoded structurally
  4. 2009 High

    Showed the NOX1 N-terminus/transmembrane domain dictates plasma-membrane localization and extracellular superoxide output, mechanistically tying topology to ROS type.

    Evidence TIRF imaging and Nox1/Nox4 chimera and signal-peptide swap mutagenesis in HEK293

    PMID:19061439

    Open questions at the time
    • No high-resolution structure of NOX1 N-terminal determinant
    • Heterologous expression system
  5. 2006 High

    Defined the activating complex by demonstrating direct Rac1 binding to NOX1 and the requirement for NOXO1/NOXA1, with NOXO1 splice variants conferring isoform specificity.

    Evidence Reciprocal Co-IP, NOXA1 TPR mutagenesis, siRNA and ROS assays; purified NOXO1 splice-variant activation assays

    PMID:15949904 PMID:16636067

    Open questions at the time
    • Stoichiometry and structure of the assembled complex unresolved
  6. 2007 High

    Revealed reversible post-translational control of complex assembly: PKA phosphorylation of NOXA1 promotes 14-3-3 binding and disassembly, defining a cAMP-gated off-switch; TNF assembles a TRADD/RIP1/NOX1/Rac1 necrosome.

    Evidence PKA site mapping and Ser-to-Ala mutants with Co-IP and ROS assays; Co-IP plus RIP1-deficient fibroblasts and dominant-negative/siRNA cell-death assays

    PMID:17560373 PMID:17913709

    Open questions at the time
    • In vivo relevance of cAMP gating not tested
    • Necrosome assembly order partially inferred
  7. 2014 High

    Identified PKCβ1 phosphorylation of NOX1-Thr429 as the licensing step enabling NOXA1 docking, linking TNFα signaling to complex assembly and SMC migration.

    Evidence Mass-spectrometry site ID, T429 mutagenesis, ITC binding measurement, PKCβ1 siRNA and migration assay

    PMID:25228390

    Open questions at the time
    • Whether Thr429 phosphorylation cooperates with Rac1/PDI inputs not integrated
  8. 2019 High

    Added a redox-dependent assembly input by showing PDI forms a Cys400–p47phox(Cys196) disulfide required for NOX1 complex formation and VSMC migration in injured arteries.

    Evidence Recombinant protein interaction, MS of crosslinked peptides, cysteine mutagenesis, in vivo proximity ligation

    PMID:30580571

    Open questions at the time
    • p47phox vs NOXO1 usage across cell types not reconciled
  9. 2016 High

    Identified Prdx6 as a NOXA1 SH3-domain partner whose peroxidase and lipase activities are required to stabilize NOXA1 and support NOX1-driven migration.

    Evidence Yeast two-hybrid, Co-IP, Prdx6 siRNA and catalytic-mutant rescue in HCT-116 cells

    PMID:27094494

    Open questions at the time
    • Mechanism by which Prdx6 catalytic activities sustain NOXA1 unresolved
  10. 2012 Medium

    Linked NOX1 to upstream receptors by demonstrating physical AT1–NOX1 association and spatially distinct activation routes (TNFα/dynamin-endosomal vs thrombin/EGFR).

    Evidence Co-IP, NOX1 siRNA, in vivo carotid model; shRNA and dominant-negative dynamin with compartment-specific ROS

    PMID:19737091 PMID:22636674

    Open questions at the time
    • Single-lab Co-IP without reciprocal structural validation
    • Receptor-to-complex recruitment steps incomplete
  11. 2011 High

    Showed NOX1 mediates thrombin/EGFR-driven matrix remodeling and SMC migration and operates in neuronal opioid signaling, broadening its physiological scope.

    Evidence NOX1-KO SMCs with re-expression rescue and EGFR inhibitor; NOX1-KO mice with GTPase, PKC translocation and RGS9-2 phosphorylation assays

    PMID:22102727 PMID:22159121

    Open questions at the time
    • Direct redox target linking NOX1 to RGS9-2 phosphorylation not identified
  12. 2013 High

    Defined NOX1's downstream redox-signaling targets and physiological roles: phosphatase oxidation (PTEN/PTP-PEST) for epithelial wound repair, Akt-cysteine oxidation for cardiomyocyte apoptosis, and genetic epistasis establishing NOX1 as the ROS source in colitis.

    Evidence Epithelial-specific and global NOX1-KO mice; phosphatase activity, Akt oxidation and PP2A Co-IP; GPx1/GPx2/Nox1 triple-KO epistasis

    PMID:22982050 PMID:23241962 PMID:24374371

    Open questions at the time
    • Direct in situ detection of phosphatase oxidation in vivo limited
  13. 2017 High

    Established NOX1 as a driver of cellular senescence via p53/p21 (SIRT1-modulated) and CD47/TSP1 inputs, and as a redox regulator of NMDA-receptor NR1 underlying depressive behavior.

    Evidence NOX1 overexpression/siRNA with SIRT1 dead-mutant; NOX1 ablation/inhibition and TSP1-KO mice; redox proteomics with NR1 C744A mutant and VTA viral delivery

    PMID:20171273 PMID:28314819 PMID:29042481

    Open questions at the time
    • How a single oxidase produces opposing senescence vs proliferation outcomes context-dependently unresolved
  14. 2019 Medium

    Connected NOX1 to lysosomal mTORC1 activation through S100A9 oxidation in colon cancer stem cells, defining a tumor-promoting redox axis.

    Evidence NOX1 siRNA/overexpression in organoids, co-localization, S100A9 oxidation, mTORC1-S100A9 Co-IP, xenografts

    PMID:31365870

    Open questions at the time
    • Single-lab Co-IP
    • Mechanism of NOX1 localization to VPS41/VPS39 lysosomes unclear
  15. 2022 Medium

    Mapped the transcriptional control of NOX1 and its organizer NOXO1 across tissues (NF-κB, STAT1/STAT3-GAS with RGS2 antagonism, MEF2B, gut GATA/HNF-1α/Cdx) and added TLR2 as an upstream binding partner.

    Evidence ChIP/promoter-reporter for STAT and GATA/HNF/Cdx; MEF2B siRNA; NF-κB/NOXO1 promoter studies; Co-IP and TLR2-KO mice

    PMID:18005670 PMID:19834108 PMID:22120521 PMID:25550204 PMID:30700829 PMID:34127487

    Open questions at the time
    • Combinatorial logic among these factors not integrated
    • Single-lab studies for most regulators
  16. 2017 Medium

    Provided human genetic evidence by validating loss-of-function NOX1 mutations that impair colonic epithelial ROS, linking NOX1 to mucosal redox at the microbiota interface.

    Evidence Exome/genome sequencing with functional ROS validation in cell lines, explants and patient-derived organoids

    PMID:29091079

    Open questions at the time
    • Causal disease link and penetrance not established
    • Mechanism connecting individual variants to clinical phenotype incomplete

Open questions

Synthesis pass · forward-looking unresolved questions
  • How NOX1 outputs are dynamically partitioned between superoxide and H2O2 and between extracellular, endosomal and lysosomal compartments to specify opposite cell fates (proliferation vs senescence vs apoptosis) remains unresolved.
  • No structure of the assembled NOX1 holoenzyme
  • Quantitative rules governing compartment- and target-specific oxidation unknown
  • In vivo identity of physiological redox substrates incompletely defined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016491 oxidoreductase activity 4
Localization
GO:0005886 plasma membrane 2 GO:0005764 lysosome 1 GO:0005768 endosome 1
Pathway
R-HSA-168256 Immune System 4 R-HSA-162582 Signal Transduction 3 R-HSA-8953897 Cellular responses to stimuli 3 R-HSA-5357801 Programmed Cell Death 2
Partners
AGTR1NOXA1NOXO1PDI (P4HB)PRDX6RAC1RIPK1TLR2
Complex memberships
NOX1–NOXO1–NOXA1–Rac1 NADPH oxidase complex

Evidence

Reading pass · 50 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 NOX1 (then called nox1/mox-1) functions as a superoxide-generating NADPH oxidase in vascular smooth muscle cells. Antisense nox1 adenovirus completely inhibited the early phase of superoxide production induced by Ang II or PDGF and significantly decreased activation of p38 MAPK and Akt by Ang II, placing Nox1 upstream of these redox-sensitive signaling molecules. Antisense adenoviral knockdown of nox1 in VSMCs; superoxide measurement; Western blot for p38 MAPK and Akt activation Circulation Research High 11348997
2001 H2O2 (not superoxide) is the primary mediator of Nox1-driven cell growth and transformation: co-expression of catalase in Nox1-expressing NIH 3T3 cells normalized H2O2 levels, reversed the transformed appearance, normalized growth rate, abolished tumorigenicity in athymic mice, and reversed the expression of >60% of Nox1-induced genes. Stable co-expression of catalase in Nox1-expressing NIH 3T3 cells; H2O2 and O2- measurement; tumor formation in athymic mice; gene expression profiling PNAS High 11331784
2002 Nox1-derived H2O2 triggers the angiogenic switch: Nox1 markedly up-regulated VEGF mRNA and induced VEGFR1/VEGFR2 in vascular cells, and co-expression of catalase eliminated Nox1-induced VEGF induction, indicating H2O2 as the signal mediating angiogenesis. Nox1 overexpression in NIH 3T3 and DU-145 cells; tumor xenograft angiogenesis assay; VEGF mRNA quantification; catalase co-expression rescue PNAS Medium 11805326
2005 Nox1 knockout mice (Nox1-/Y) show significantly blunted blood pressure elevation and reduced aortic superoxide production in response to Ang II infusion compared with wild-type, demonstrating that Nox1-derived ROS mediate the pressor response to Ang II by reducing nitric oxide bioavailability (endothelium-dependent relaxation and cGMP preserved in Nox1-/Y). Nox1 knockout mice; telemetric blood pressure monitoring; superoxide measurement; endothelium-dependent relaxation assays; cGMP measurement; L-NAME co-treatment Circulation High 16246966
2006 Nox1 is directly activated by the small GTPase Rac1: constitutively active Rac1(G12V), but not wild-type Rac1 or CDC42, markedly stimulated Nox1-dependent ROS generation; Rac1 siRNA reduced Nox1-dependent ROS; Rac1(G12V) co-immunoprecipitated with Nox1, NOXO1, and NOXA1; NOXA1 TPR domain mutants that block Rac1 binding abolished Nox1 activity; and Rac1(G12V) formed a complex with Nox1 independently of NOXO1/NOXA1, indicating direct binding. Co-expression of Rac1/CDC42 mutants with Nox1/NOXO1/NOXA1 in HEK293 cells; ROS measurement; co-immunoprecipitation; siRNA knockdown; NOXA1 TPR domain mutagenesis Journal of Biological Chemistry High 16636067
2007 PKA phosphorylates NoxA1 (the Nox1 activator) at Ser172 and Ser461, enhancing NoxA1 association with 14-3-3 proteins, causing dissociation of NoxA1 from the Nox1 complex at the plasma membrane, thereby inhibiting Nox1-dependent ROS production. Elevation of cAMP inhibited Nox1 activity, while PKA inhibition enhanced it, and 14-3-3ζ availability intensified this inhibitory effect. PKA phosphorylation site mapping; site-directed mutagenesis (Ser172A, Ser461A); co-immunoprecipitation; ROS measurement in HEK293 and colon cell lines; cAMP elevation; 14-3-3 binding assays Journal of Biological Chemistry High 17913709
2007 TNF treatment induces formation of a signaling complex containing TRADD, RIP1, Nox1, and Rac1 in mouse fibroblasts undergoing necrosis. RIP1-deficient fibroblasts fail to form this complex, indicating RIP1 is essential for Nox1 recruitment. Dominant-negative TRADD or Rac1, or Nox1 siRNA knockdown, inhibited TNF-induced superoxide generation and necrotic cell death. Co-immunoprecipitation; dominant-negative TRADD/Rac1 expression; Nox1 siRNA; RIP1-deficient fibroblasts; cell death assays Molecular Cell High 17560373
2008 Nox1 and Nox4 produce distinct reactive oxygen species: Nox1 depletion (antisense adenovirus) in rat aortic smooth muscle cells inhibited Ang II-stimulated superoxide production and H2O2 in membrane fractions and intact cells, whereas Nox4 depletion (siRNA) reduced basal H2O2 production but not Ang II-stimulated responses. This establishes that Nox1 generates predominantly superoxide that is subsequently converted to H2O2. Antisense Nox1 adenovirus; Nox4 siRNA; ESR spin-trapping; Amplex Red H2O2 assay; dihydroethidium; membrane fraction NADPH oxidase activity assay Free Radical Biology & Medicine High 18760347
2009 The N-terminal domain of Nox1 determines its plasma membrane localization and the type of ROS released extracellularly (superoxide): TIRF microscopy showed Nox1 localizes to the plasma membrane while Nox4 colocalizes with ER markers. Chimeric Nox1/Nox4 proteins showed that the cytosolic tail of Nox4 fused to the transmembrane part of Nox1 rendered Nox1 constitutively active, and that inserting the Nox4 signal peptide into Nox1 shifted localization from plasma membrane to vesicle-like structures and switched extracellular ROS from superoxide to H2O2. TIRF microscopy; chimeric Nox1/Nox4 protein construction; ROS type measurement; N-terminal signal peptide swap mutagenesis; HEK293 expression system Antioxidants & Redox Signaling High 19061439
2009 STAT1 and STAT3, activated via the JAK/STAT pathway, directly bind GAS elements in the NOX1 promoter and transcriptionally regulate Nox1 expression in human aortic smooth muscle cells. Chromatin immunoprecipitation confirmed physical interaction of STAT1/STAT3 with NOX1 promoter GAS elements; JAK/STAT inhibitors reduced IFNγ-induced Nox1 and Nox4 expression and NADPH oxidase activity. Chromatin immunoprecipitation (ChIP); promoter-reporter assays; STAT1/STAT3 overexpression; JAK/STAT inhibitors; real-time PCR; Western blot Arteriosclerosis, Thrombosis, and Vascular Biology High 19834108
2010 TNF-α activates Nox1 via dynamin-dependent endocytosis to generate endosomal ROS, whereas thrombin activates Nox1 through EGFR transactivation without endosomal ROS generation. Both ligands required Nox1 and dynamin for PI3K-Akt-ATF-1 pathway activation, establishing spatially distinct mechanisms of Nox1 activation by different receptor-ligand complexes. Nox1 shRNA; dominant-negative dynamin; EGFR inhibitor AG1478; PI3K inhibitor; ROS compartment-specific measurements in smooth muscle cells Antioxidants & Redox Signaling Medium 19737091
2010 Nox1-dependent superoxide production controls colon adenocarcinoma cell (HT29-D4) migration on collagen-I via regulation of α2β1 integrin membrane availability. Upstream, arachidonic acid metabolism through 12-lipoxygenase and protein kinase C delta activates Nox1, and 12-HETE (the 12-LOX product) restored Nox1-dependent superoxide and migration when 12-LOX was inhibited. Nox1 siRNA; 12-LOX inhibitors; PKC isoform inhibitors; superoxide measurement; integrin membrane availability assay; Boyden chamber migration Biochimica et Biophysica Acta Medium 18023288
2010 Nox1 inhibits p53 transcriptional activity and proapoptotic function via a SIRT1-dependent mechanism: Nox1 inhibited p53 Lys382 acetylation (a SIRT1 target), impaired p53 proapoptotic transcriptional activity, and could not inhibit p53 when co-expressed with SIRT1 deacetylase-defective mutant (SIRT1HY), establishing a functional link between Nox1, SIRT1, and p53 acetylation. Nox1 overexpression and siRNA; SIRT1 siRNA and pharmacological inhibition (nicotinamide); SIRT1HY deacetylase-dead mutant co-expression; p53 acetylation (Lys382) assay; p53 reporter assay Free Radical Biology & Medicine Medium 20171273
2010 A specific small-molecule inhibitor (ML171/2-acetylphenothiazine) selectively inhibits Nox1-dependent ROS generation at nanomolar concentrations with minimal activity on other Nox isoforms or other cellular ROS-producing enzymes. ML171 blocks ROS-dependent formation of invadopodia in colon cancer cells, and this effect is reversed by Nox1 overexpression, confirming selectivity. High-throughput screening; isoform-specific ROS assays; invadopodium formation assay; Nox1 overexpression rescue ACS Chemical Biology High 20715845
2011 Nox1 mediates thrombin-induced EGFR transactivation, MMP-9 activation, N-cadherin shedding, and smooth muscle cell migration: Nox1 knockout SMCs failed to show thrombin-induced Src phosphorylation, EGFR transactivation, ERK1/2 and MMP-9 activation, or N-cadherin shedding, and SMC migration was rescued by Nox1 re-expression in KO cells. EGFR inhibitor AG1478 blocked Nox1-dependent SMC migration. Nox1 knockout mouse-derived SMCs; adenoviral Nox1 re-expression rescue; EGFR inhibitor; ROS measurement; phosphorylation assays; migration assay Cardiovascular Research High 22102727
2011 NOX1 is expressed in hepatic stellate cells (but not Kupffer cells) and mediates Ang II-induced profibrogenic effects in liver: NOX1 knockout HSCs had decreased ROS generation and failed to up-regulate collagen α1(I) and TGFβ in response to Ang II. Chimeric mouse studies showed NOX1 mediates profibrogenic effects in endogenous liver cells. NOX1 knockout mice; bone marrow chimeras; in vitro HSC activation; ROS measurement; collagen and TGFβ expression assays; hepatic fibrosis models (CCl4, bile duct ligation) Hepatology High 21384410
2011 Nox1 mediates morphine-induced analgesic tolerance by regulating GTPase activity and PKC-mediated phosphorylation of RGS9-2, leading to Gαi2/RGS9-2/14-3-3 complex formation: Nox1-/Y mice showed enhanced morphine analgesia, suppressed analgesic tolerance, attenuated morphine-stimulated GTPase activity, abolished PKC translocation, and reduced RGS9-2 phosphorylation and Gαi2/RGS9-2/14-3-3 complex formation. Nox1 knockout mice; [35S]GTPγS binding; GTPase activity assay; PKC translocation assay; RGS9-2 phosphorylation; co-immunoprecipitation; opioid receptor binding ([3H]DAMGO) The Journal of Neuroscience High 22159121
2012 NOX1 is required for TNFα-induced ROS secretion in intestinal epithelial cells and for suppression of M cell differentiation. NOX1-deficient colonoids exposed to TNFα showed markedly reduced ROS production and induced an M cell transcriptional program; M cell induction was rescued by addition of exogenous H2O2 or paraquat, demonstrating roles for both extra- and intracellular ROS in maintaining epithelial identity. NOX1-deficient murine colonoids; TNFα stimulation; ROS measurement; scRNASeq; immunohistochemistry (UEA1); H2O2 and paraquat rescue; in vivo DSS injury model Gut High 36191961
2012 NOX1 is involved in endotoxin-induced cardiomyocyte apoptosis through oxidation of Akt cysteine residues and subsequent enhanced Akt-PP2A interaction leading to Akt dephosphorylation: LPS-treated Nox1-/Y mice showed reduced cardiac dysfunction and cardiomyocyte apoptosis, attenuated Akt cysteine oxidation, and reduced Akt-PP2A association compared to wild-type. Nox1 knockout mice; LPS and CLP models; caspase-3 activation assay; Akt phosphorylation; Akt cysteine oxidation measurement; Akt-PP2A co-immunoprecipitation Free Radical Biology & Medicine High 22982050
2012 Annexin A1 (ANXA1) activates epithelial FPR1 receptors, triggering NOX1-dependent ROS generation which inactivates PTEN and PTP-PEST phosphatases (oxidative inactivation), leading to FAK and paxillin activation and intestinal epithelial cell migration/wound repair. Intestinal epithelial-specific Nox1-/- mice showed defective mucosal wound repair. Intestinal epithelial specific Nox1-/- mice; AnxA1-/- mice; phosphatase activity assay; FAK/paxillin phosphorylation; epithelial migration assay; systemic ANXA1 administration Journal of Clinical Investigation High 23241962
2012 H2AX increases Nox1 activity partly by reducing the interaction between the Nox1 activator NOXA1 and its inhibitor 14-3-3ζ: DNA damage-induced ROS generation was blocked by Rac1N17 dominant-negative expression and Nox1 siRNA (but not Nox4 siRNA), and H2AX overexpression alone increased Nox1-dependent ROS, placing Nox1 downstream of H2AX in the DNA damage response. H2AX overexpression; Nox1 and Nox4 siRNA; Rac1N17 dominant-negative; ROS measurement (NAC, DPI); NOXA1/14-3-3ζ co-immunoprecipitation Cell Death & Disease Medium 22237206
2012 AT1 receptor physically associates with Nox1 in vascular smooth muscle cells, and Ang II treatment enhances this binding. Nox1 knockdown attenuated Ang II-induced superoxide generation, NF-κB/AP-1 activation, IL-18 and MMP-9 induction, and SMC migration and proliferation. Co-immunoprecipitation (AT1/Nox1); Nox1 siRNA; superoxide measurement; NF-κB/AP-1 reporter assays; SMC migration and proliferation assays; in vivo rat carotid infusion model American Journal of Physiology – Heart and Circulatory Physiology Medium 22636674
2012 Extracellular H2O2 enters smooth muscle cells via aquaporin 1 (Aqp1) and activates Nox1-derived superoxide production and Ask1, leading to SMC hypertrophy. Aqp1 siRNA attenuated H2O2 cellular entry and Nox1-dependent superoxide; Nox1 siRNA abrogated Ask1 activation and hypertrophy. Nox1 siRNA; Aqp1 siRNA; dominant-negative Ask1 adenovirus; superoxide measurement (EPR, cytochrome c); H2O2 cellular entry assay Cardiovascular Research Medium 22997161
2014 Protein kinase C-β1 phosphorylates Nox1 at threonine 429 in response to TNF-α, and this phosphorylation facilitates association of Nox1 with the NoxA1 activation domain, enabling NADPH oxidase complex assembly, ROS production, and VSMC migration. PKCβ1 siRNA abolished TNF-α-mediated ROS and migration; T429 mutagenesis prevented complex assembly. Mass spectrometry phosphorylation site identification; site-directed mutagenesis (T429); isothermal titration calorimetry (NoxA1 binding affinity); PKCβ1 siRNA; ROS measurement; VSMC migration assay Circulation Research High 25228390
2014 Nox1 is required for GPVI-dependent ROS production in platelets and downstream p38 MAPK activation and TxA2 production, but is not required for platelet aggregation, integrin αIIbβ3 activation, spreading, or granule release. Both Nox1 and Nox2 contribute to collagen-mediated thrombus formation at arterial shear. ML171 (Nox1-specific inhibitor); Nox2-deficient mice; CRP agonist activation; TxA2 measurement; p38 MAPK phosphorylation; platelet aggregation; ex vivo perfusion Redox Biology Medium 24494191
2014 The MEF2B transcription factor mediates pathological cyclic stretch-induced Nox1 mRNA and protein upregulation in VSMCs, and Nox1-derived ROS drive a phenotypic switch from contractile to synthetic state (increased osteopontin, decreased calponin1 and smoothelin B, increased MMP-9 activity, and enhanced migration). MEF2B siRNA; isoform-specific Nox1 inhibitor; Nox1 gene silencing; cyclic stretch apparatus; qPCR; VSMC phenotypic marker assays; MMP-9 activity; migration assay Arteriosclerosis, Thrombosis, and Vascular Biology Medium 25550204
2015 NOX1 promotes premature senescence of VSMCs induced by Ang II and zinc via an NF-κB and mitochondrial ROS-dependent transcriptional mechanism: Nox1 siRNA prevented senescence; Nox1 overexpression induced senescence with DNA damage and reduced telomerase. Zinc increased Nox1 protein through mitochondrial ROS → NF-κB → Nox1 transcription pathway, and Nox1 upregulation was zinc-specific compared with other metals. Nox1 siRNA; Nox1 overexpression; MitoTEMPO (mitochondrial ROS scavenger); NF-κB inhibition; ZnT3/ZnT10 zinc exporter overexpression; SA-β-gal senescence assay; DNA damage markers Free Radical Biology & Medicine Medium 28363602
2015 NOX1 mediates premature senescence in early-stage diabetic kidney by activating PKCα/β translocation and p38 MAPK phosphorylation, leading to p27Kip1 accumulation and DNA damage (γ-H2AX). Nox1-deficient mice showed reduced membranous PKC translocation, PKC activity, p38 phosphorylation, p27Kip1 levels, and γ-H2AX in diabetic kidney. Nox1 knockout mice; streptozotocin diabetes model; PKC activity and translocation assays; p38 MAPK phosphorylation; SA-β-gal staining; γ-H2AX immunostaining Free Radical Biology & Medicine Medium 25701431
2016 Peroxiredoxin 6 (Prdx6) is a novel binding partner of Noxa1 (Nox activator 1, the Nox1 activator subunit), identified by yeast two-hybrid screening using the Noxa1 SH3 domain as bait. Prdx6 binds to and stabilizes wild-type Noxa1 but not the SH3 domain mutant Noxa1 W436R; Prdx6 knockdown suppresses Nox1 activity; both peroxidase (C47S) and lipase (S32A) mutant forms of Prdx6 fail to support Nox1-dependent superoxide generation or Nox1-mediated cell migration. Yeast two-hybrid screening; co-immunoprecipitation; Prdx6 siRNA; Prdx6 mutant overexpression (C47S, S32A); Nox1 activity assay; wound closure migration assay in HCT-116 cells; MJ-33 Prdx6 PLA2 inhibitor Free Radical Biology & Medicine High 27094494
2017 Thrombospondin 1 (TSP1), acting through its receptor CD47, activates Nox1 (but not other closely related Nox isoforms) to generate ROS, increase p53 abundance, and drive endothelial senescence via Rb/p21cip. Nox1 inhibition blocked TSP1-induced p53 nuclear localization and p21cip induction; mice lacking TSP1 showed reduced ROS, p21cip, p53 activity, and senescence. Nox1 genetic ablation; Nox1 pharmacological inhibitor; CD47 blockade; TSP1 knockout mice; p53/p21cip/Rb assays; senescence assays in human endothelial cells and aged tissue Science Signaling High 29042481
2017 NOX1-derived ROS in the ventral tegmental area mediates depressive-like behaviors through redox modification of NMDA receptor subunit NR1: redox proteomics showed NR1 is redox-regulated by NOX1; H2O2 suppressed NMDA-induced BDNF upregulation in NR1-expressing neurons but not in cells expressing cysteine-mutant NR1 (C744A); NOX1 promotes epigenetic silencing (DNA methylation) of bdnf promoter in PFC. Nox1 knockout mice; redox proteomics; NR1 C744A mutant expression; miRNA-NOX1 delivery to VTA; BDNF mRNA and DNA methylation assays; chronic social defeat and corticosterone depression models The Journal of Neuroscience High 28314819
2019 PDI (protein disulfide isomerase A1) activates Nox1 through a redox-dependent intermolecular disulfide bond between PDI catalytic Cys400 and p47phox Cys196: mass spectrometry confirmed disulfide bonds between PDI and p47phox cysteines; mutation of these cysteines prevented Nox1 complex assembly and VSMC migration. Proximity ligation assay confirmed the PDI-p47phox interaction in murine carotid arteries after wire injury. Recombinant protein in vitro interaction; mass spectrometry of crosslinked peptides; site-directed mutagenesis (PDI Cys400, p47phox Cys196); Nox1 complex assembly assay; VSMC migration; proximity ligation assay in vivo Arteriosclerosis, Thrombosis, and Vascular Biology High 30580571
2019 NOX1 co-localizes with mTORC1 in VPS41/VPS39-positive lysosomes in colon cancer stem cells. NOX1-derived ROS oxidize S100A9, facilitating its binding to mTORC1 and mTORC1 activation, thereby driving CSC proliferation and colon cancer progression. Nox1 siRNA/overexpression in spheroids and organoids; co-localization immunofluorescence; mTORC1 activity assay; S100A9 oxidation measurement; mTORC1-S100A9 co-immunoprecipitation; xenograft tumor models Cell Reports Medium 31365870
2019 Hypoxia-induced Nox1 drives endothelial proliferation and migration in pulmonary arterial hypertension through a Nox1-PKA-CREB/Ref-1 signaling pathway that leads to Gremlin1 transcription: ChIP assay showed CREB binding to the Gremlin1 promoter; Nox1 inhibition (NoxA1ds peptide inhibitor or siRNA) abrogated hypoxia-induced PKA activity, CREB phosphorylation, CREB:CRE binding, and Gremlin1 expression. Nox1 siRNA; NoxA1ds peptide inhibitor; ChIP assay for CREB:CRE binding; CREB gene silencing; PKA activity assay; EC proliferation and migration; rat PAH model in vivo Redox Biology Medium 30802716
2021 NOX1 expression in colonic stem cells is regulated by Toll-like receptor activation in response to microbiota components, and NOX1-derived ROS mediate EGFR activation to drive CSC proliferation. In the absence of NOX1, CSCs fail to generate ROS and have reduced proliferation; the TLR-NOX1-EGFR axis links bacterial sensing to stem cell proliferation. NOX1-deficient colonoids and mice; ROS measurement; EGFR inhibition; TLR agonist stimulation; CSC proliferation assay; in vivo germ-free/microbiota experiments Cell Reports Medium 33826887
2021 NOX1-derived ROS are critical for spermatogonial stem cell (SSC) self-renewal under normoxia through a ROS-BCL6B-NOX1 feed-forward pathway. Under hypoxia, NOX1-derived ROS are reduced (despite increased mitochondrial ROS), and Nox1-deficient SSCs proliferate poorly under hypoxia but normally under normoxia. NOX1-derived ROS also regulate HIF1A expression in undifferentiated spermatogonia. Nox1 knockout mice; SSC culture under normoxia/hypoxia; ROS measurement; BCL6B expression; HIF1A expression; CDKN1A genetic interaction (Cdkn1a-deficient rescue) Genes & Development Medium 33446567
2021 Platelet-derived extracellular vesicles (PDEVs) contain Nox1 and generate Nox1-dependent superoxide. PDEV-mediated platelet activation is abrogated by Nox1 inhibition with ML171, establishing Nox1 as a functional enzyme in PDEVs that mediates their platelet-activating capacity. Flow cytometry; immunoblot; EPR for superoxide; ML171 Nox1 inhibitor; Nox1 colocalization assay; PDEV platelet activation readout Free Radical Biology & Medicine Medium 33548451
2022 BMP4 induces time-dependent physical binding between TLR2 and NOX1 (and TLR2 with NOXO1) in aortic endothelial cells. TLR2 knockout mice were protected from high-fat diet-induced endothelial dysfunction and NOX1-dependent superoxide production, establishing TLR2 as an upstream binding partner required for NOX1 activation in endothelial cells. Co-immunoprecipitation (TLR2/NOX1 and TLR2/NOXO1); TLR2 knockout mice; high-fat diet metabolic model; eNOS uncoupling assay; superoxide measurement; endothelium-dependent vasorelaxation Diabetes Medium 34127487
2022 Renal NOXA1/NOX1 signaling in the distal nephron regulates epithelial sodium channel (ENaC) expression and Na+ reabsorption during Ang II-induced hypertension: Ang II increased NOXA1/NOX1 expression and ROS in male kidney; Noxa1-deficient mice showed delayed Na+ excretion and blunted ENaC upregulation. Aldosterone induced Nox1-dependent ENaC activity in renal epithelial cells, and Noxa1 siRNA abolished this. Noxa1 knockout mice; telemetric blood pressure; Na+ excretion assay; ENaC expression and patch-clamp assay; aldosterone stimulation; Noxa1 siRNA in renal epithelial cells Antioxidants & Redox Signaling Medium 34714114
2005 NOXO1 splice variants (NOXO1β and NOXO1γ) differentially regulate Nox family members: both activate Nox1, but NOXO1γ shows a reduced ability to activate Nox3 compared to NOXO1β, establishing that NOXO1 splice forms have distinct functional specificities for different Nox isoforms. Expression of purified NOXO1 splice variant proteins; Nox1 and Nox3 activation assays; PX domain lipid-binding assay Gene Medium 15949904
2007 GATA-4/5/6, HNF-1α, and Cdx1/Cdx2 transcription factors cooperatively regulate NOX1 transcription by binding a critical promoter element between -422 and -291 in colon epithelial cells. ChIP confirmed physical interaction of GATA-6, HNF-1α, and Cdx2 with this region in intact chromatin; these factors demonstrated cooperativity in transactivating the NOX1 promoter. Promoter-reporter deletion analysis; ChIP; EMSA; transcription factor overexpression; real-time PCR; in vivo expression gradient analysis Free Radical Biology & Medicine Medium 18005670
2011 RGS2 directly binds STAT3 in the nucleus and suppresses STAT3-mediated Nox1 transcription. TLR2 signaling enhances Nox1 expression through the JAK1/JAK3-STAT3 pathway in a MyD88-independent manner; TLR2 represses RGS2, and Nox1 induction upon RGS2 downregulation is mediated by PKC-η and phospholipase D2. RGS2-STAT3 co-immunoprecipitation; GFP-RGS2 nuclear localization imaging; Nox1 promoter reporter; PKC-η and PLD2 inhibitors/knockdown; TLR2 stimulation; JAK inhibitor Cellular Signalling Medium 22120521
2012 Nox1-derived ROS in rat REF52 cells and Nox4-derived ROS in human TIG-3 fibroblasts are induced by the Ras/MEK pathway and are required for RasV12-induced premature senescence: Nox1 and Nox4 siRNA blocked RasV12 senescent phenotype (β-galactosidase, growth arrest, p53/p16Ink4a accumulation), DNA damage response, and p38 MAPK activation. Nox1 knockout MEFs confirmed the role of Nox1 in Ras-induced senescence. Nox1/Nox4 siRNA; Nox1 knockout MEFs; RasV12 overexpression; SA-β-gal assay; p53/p16Ink4a Western blot; DNA damage and p38 MAPK assays Genes to Cells Medium 23216904
2013 Nox1 causes ileocolitis in GPx1/GPx2-double knockout mice: GPx1/GPx2/Nox1 triple knockout male mice were virtually disease-free compared to GPx1/GPx2 DKO mice, with dramatically reduced crypt apoptosis, reduced Ki-67+ crypt epithelium, reduced monocyte/myeloperoxidase staining, and ~8-fold lower TNF cytokine levels in ileum. This establishes Nox1 as the causative source of intestinal oxidative stress and inflammation in this model. Triple knockout mice (GPx1/GPx2/Nox1); histological analysis; TUNEL; Ki-67; cleaved caspase-3 immunohistochemistry; TNF cytokine measurement; intestinal length measurement Free Radical Biology & Medicine High 24374371
2017 Loss-of-function mutations in NOX1 impair ROS production in colonic epithelium. Validated mutations (p.N122H, p.T497A, p.Y470H, p.R287Q, p.I67M, p.Q293R, p.P330S) reduced ROS production in cell lines, ex vivo colonic explants, and patient-derived colonic organoids. NOX1 constitutively generates high ROS levels in the colonic crypt lumen at the epithelium-microbiota interface. Whole-genome and exome sequencing; ROS production assays in cell lines, colonic explants, and patient-derived organoids; NOX1 mutation functional validation Mucosal Immunology Medium 29091079
2018 NOX1-derived ROS drive LCN-2 (Lipocalin-2) expression in colonic epithelial cells by controlling IκBζ expression (a master inducer of LCN-2). TNFα + IL-17 induced NOXO1 expression via p38MAPK and JNK1/2, increasing NOX1 activity and ROS; NOX1-deficient mice showed decreased LCN-2 and reduced colon damage during TNBS-induced colitis. NOX1-deficient mice; NOXO1 siRNA; p38MAPK/JNK inhibitors; IκBζ expression analysis; LCN-2 measurement; TNBS colitis model Mucosal Immunology Medium 30279516
2019 NF-κB directly regulates NOXO1 (the Nox1 organizer subunit) expression in TNF-α-stimulated gastric cancer cells, activating the NOX1 complex and increasing ROS. NOX1/ROS signaling increases SOX2-positive undifferentiated gastric epithelial cells, and disruption of Noxo1 in K19-C2mE mice suppressed metaplastic hyperplasia and decreased SOX2-positive cell numbers. NF-κB inhibition; NOXO1 promoter analysis; NOX1/NOXO1 siRNA; in situ hybridization; Noxo1 gene disruption in K19-C2mE mice; SOX2 immunostaining; pharmacological NOX inhibition Oncogene Medium 30700829
2020 TSPO is a key upstream regulator of NOX1-dependent neurotoxic ROS production in retinal microglia/phagocytes. Using NADPH oxidase-deficient mice, conditional TSPO deletion in resident microglia (Cx3cr1CreERT2:TSPOfl/fl), and TSPO ligand XBD173 treatment, TSPO was shown to control retinal phagocyte reactivity and subsequent NOX1-dependent neovascularization in a laser-induced AMD model. Conditional TSPO knockout mice; NADPH oxidase-deficient mice; tamoxifen-induced Cre-mediated deletion; XBD173 ligand treatment; ROS measurement; neovascularization assay in laser-induced AMD model Nature Communications Medium 32483169
2021 NOX1 drives a feedforward inflammatory loop with IL-6 (a SASP component) in vascular senescence: selective NOX1 inhibition in vivo completely reversed age-impaired hind-limb blood flow and angiogenesis while disrupting the NOX1-IL-6 SASP proinflammatory signaling loop. PPARγ down-regulation inversely modulates p65-mediated NOX1 transcription. Selective NOX1 inhibitor in aged mice; hind-limb blood flow measurement; angiogenesis assay; IL-6/SASP measurement; PPARγ/p65 transcriptional regulation assay; endothelial cell wound response assay PNAS Medium 34654740
2006 NOX1-derived superoxide negatively regulates NGF-induced neurite outgrowth in PC12 cells: NADPH oxidase inhibitors and superoxide scavengers significantly enhanced NGF-induced neurite outgrowth, and stable ribozyme-mediated knockdown of NOX1 mRNA similarly enhanced neurite outgrowth and β-III tubulin expression. NGF induction of NOX1 was mediated via TrkA. Stable ribozyme-mediated NOX1 knockdown; NADPH oxidase inhibitors (DPI); superoxide scavengers; neurite length measurement; β-III tubulin expression; PI3K inhibitor studies Free Radical Biology & Medicine Medium 16678016

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 Novel gp91(phox) homologues in vascular smooth muscle cells : nox1 mediates angiotensin II-induced superoxide formation and redox-sensitive signaling pathways. Circulation research 720 11348997
2007 TNF-induced activation of the Nox1 NADPH oxidase and its role in the induction of necrotic cell death. Molecular cell 430 17560373
2005 Nox1 is involved in angiotensin II-mediated hypertension: a study in Nox1-deficient mice. Circulation 416 16246966
2002 Reactive oxygen generated by Nox1 triggers the angiogenic switch. Proceedings of the National Academy of Sciences of the United States of America 397 11805326
2001 Hydrogen peroxide mediates the cell growth and transformation caused by the mitogenic oxidase Nox1. Proceedings of the National Academy of Sciences of the United States of America 396 11331784
2008 Distinct roles of Nox1 and Nox4 in basal and angiotensin II-stimulated superoxide and hydrogen peroxide production. Free radical biology & medicine 320 18760347
2012 Annexin A1, formyl peptide receptor, and NOX1 orchestrate epithelial repair. The Journal of clinical investigation 251 23241962
2012 DNA damage induces reactive oxygen species generation through the H2AX-Nox1/Rac1 pathway. Cell death & disease 250 22237206
2005 Increased Nox1 and hydrogen peroxide in prostate cancer. The Prostate 249 15389790
2006 Nox1-dependent reactive oxygen generation is regulated by Rac1. The Journal of biological chemistry 239 16636067
2000 A mammalian H+ channel generated through alternative splicing of the NADPH oxidase homolog NOH-1. Science (New York, N.Y.) 238 10615049
2011 The nicotinamide adenine dinucleotide phosphate oxidase (NOX) homologues NOX1 and NOX2/gp91(phox) mediate hepatic fibrosis in mice. Hepatology (Baltimore, Md.) 172 21384410
2005 Expression of NOX1, a superoxide-generating NADPH oxidase, in colon cancer and inflammatory bowel disease. The Journal of pathology 169 16086438
2010 A novel and specific NADPH oxidase-1 (Nox1) small-molecule inhibitor blocks the formation of functional invadopodia in human colon cancer cells. ACS chemical biology 158 20715845
2009 Roles of Nox1 and other Nox isoforms in cancer development. Cancer science 148 19493276
2008 Nox1 is over-expressed in human colon cancers and correlates with activating mutations in K-Ras. International journal of cancer 134 18398843
2009 Identification of structural elements in Nox1 and Nox4 controlling localization and activity. Antioxidants & redox signaling 125 19061439
2009 Jak/STAT signaling pathway regulates nox1 and nox4-based NADPH oxidase in human aortic smooth muscle cells. Arteriosclerosis, thrombosis, and vascular biology 125 19834108
2019 Differential contribution of Nox1, Nox2 and Nox4 to kidney vascular oxidative stress and endothelial dysfunction in obesity. Redox biology 105 31563085
2012 Integration of pro-inflammatory cytokines, 12-lipoxygenase and NOX-1 in pancreatic islet beta cell dysfunction. Molecular and cellular endocrinology 102 22502743
2005 Expression of NADPH oxidase isoform 1 (Nox1) in human placenta: involvement in preeclampsia. Placenta 100 15993942
2014 The role of Nox1 and Nox2 in GPVI-dependent platelet activation and thrombus formation. Redox biology 99 24494191
2011 NOX1, 2, 4, 5: counting out oxidative stress. British journal of pharmacology 99 21323893
2012 ROS-generating oxidases Nox1 and Nox4 contribute to oncogenic Ras-induced premature senescence. Genes to cells : devoted to molecular & cellular mechanisms 90 23216904
2018 Carcinogenesis and Reactive Oxygen Species Signaling: Interaction of the NADPH Oxidase NOX1-5 and Superoxide Dismutase 1-3 Signal Transduction Pathways. Antioxidants & redox signaling 88 29478325
2012 Angiotensin II enhances AT1-Nox1 binding and stimulates arterial smooth muscle cell migration and proliferation through AT1, Nox1, and interleukin-18. American journal of physiology. Heart and circulatory physiology 86 22636674
2007 Regulation of Nox1 activity via protein kinase A-mediated phosphorylation of NoxA1 and 14-3-3 binding. The Journal of biological chemistry 85 17913709
2007 Nox1-dependent superoxide production controls colon adenocarcinoma cell migration. Biochimica et biophysica acta 84 18023288
2017 The matricellular protein TSP1 promotes human and mouse endothelial cell senescence through CD47 and Nox1. Science signaling 83 29042481
2006 Redox-dependent expression of cyclin D1 and cell proliferation by Nox1 in mouse lung epithelial cells. Antioxidants & redox signaling 83 16987002
2014 MEF2B-Nox1 signaling is critical for stretch-induced phenotypic modulation of vascular smooth muscle cells. Arteriosclerosis, thrombosis, and vascular biology 81 25550204
2017 Zinc regulates Nox1 expression through a NF-κB and mitochondrial ROS dependent mechanism to induce senescence of vascular smooth muscle cells. Free radical biology & medicine 78 28363602
2017 NOX1 loss-of-function genetic variants in patients with inflammatory bowel disease. Mucosal immunology 77 29091079
2011 Differential regulation of Nox1, Nox2 and Nox4 in vascular smooth muscle cells from WKY and SHR. Journal of the American Society of Hypertension : JASH 75 21419746
2008 A combination of Lox-1 and Nox1 regulates TLR9-mediated foam cell formation. Cellular signalling 70 18817866
2015 Mouse lung development and NOX1 induction during hyperoxia are developmentally regulated and mitochondrial ROS dependent. American journal of physiology. Lung cellular and molecular physiology 69 26092998
2012 NOX1/NADPH oxidase is involved in endotoxin-induced cardiomyocyte apoptosis. Free radical biology & medicine 69 22982050
2010 A differential role for endocytosis in receptor-mediated activation of Nox1. Antioxidants & redox signaling 69 19737091
2007 Evidence for cancer-associated expression of NADPH oxidase 1 (Nox1)-based oxidase system in the human stomach. Free radical biology & medicine 68 18037128
2015 NADPH oxidase NOX1 is involved in activation of protein kinase C and premature senescence in early stage diabetic kidney. Free radical biology & medicine 67 25701431
2011 Nox1 transactivation of epidermal growth factor receptor promotes N-cadherin shedding and smooth muscle cell migration. Cardiovascular research 67 22102727
2022 Osteopontin promotes hepatocellular carcinoma progression through inducing JAK2/STAT3/NOX1-mediated ROS production. Cell death & disease 63 35418176
2020 The TSPO-NOX1 axis controls phagocyte-triggered pathological angiogenesis in the eye. Nature communications 63 32483169
2012 Aquaporin 1, Nox1, and Ask1 mediate oxidant-induced smooth muscle cell hypertrophy. Cardiovascular research 63 22997161
2010 Nox1 is involved in p53 deacetylation and suppression of its transcriptional activity and apoptosis. Free radical biology & medicine 63 20171273
2019 NF-κB-induced NOX1 activation promotes gastric tumorigenesis through the expansion of SOX2-positive epithelial cells. Oncogene 60 30700829
2018 NOX1-derived ROS drive the expression of Lipocalin-2 in colonic epithelial cells in inflammatory conditions. Mucosal immunology 60 30279516
2019 SHMT1 inhibits the metastasis of HCC by repressing NOX1-mediated ROS production. Journal of experimental & clinical cancer research : CR 59 30755243
2016 Nox1 in cardiovascular diseases: regulation and pathophysiology. Clinical science (London, England : 1979) 59 26678171
2017 Depressive-Like Behaviors Are Regulated by NOX1/NADPH Oxidase by Redox Modification of NMDA Receptor 1. The Journal of neuroscience : the official journal of the Society for Neuroscience 58 28314819
2006 NOX1/NADPH oxidase negatively regulates nerve growth factor-induced neurite outgrowth. Free radical biology & medicine 57 16678016
2014 Angiotensin II induces Fat1 expression/activation and vascular smooth muscle cell migration via Nox1-dependent reactive oxygen species generation. Journal of molecular and cellular cardiology 53 24445059
2004 Nox1 regulates apoptosis and potentially stimulates branching morphogenesis in sinusoidal endothelial cells. Experimental cell research 53 15475009
2022 NOX1 is essential for TNFα-induced intestinal epithelial ROS secretion and inhibits M cell signatures. Gut 51 36191961
2009 HO-1 and CO decrease platelet-derived growth factor-induced vascular smooth muscle cell migration via inhibition of Nox1. Arteriosclerosis, thrombosis, and vascular biology 51 19875720
2021 NOX1-dependent redox signaling potentiates colonic stem cell proliferation to adapt to the intestinal microbiota by linking EGFR and TLR activation. Cell reports 49 33826887
2016 Peroxiredoxin 6 (Prdx6) supports NADPH oxidase1 (Nox1)-based superoxide generation and cell migration. Free radical biology & medicine 49 27094494
2011 Involvement of NOX1/NADPH oxidase in morphine-induced analgesia and tolerance. The Journal of neuroscience : the official journal of the Society for Neuroscience 49 22159121
2008 Nox1 downstream of 12-lipoxygenase controls cell proliferation but not cell spreading of colon cancer cells. International journal of cancer 49 18076063
2023 Rhein Inhibited Ferroptosis and EMT to Attenuate Diabetic Nephropathy by Regulating the Rac1/NOX1/β-Catenin Axis. Frontiers in bioscience (Landmark edition) 48 37258467
2017 Endothelial Nox1 oxidase assembly in human pulmonary arterial hypertension; driver of Gremlin1-mediated proliferation. Clinical science (London, England : 1979) 48 28522681
2015 Unchanged NADPH Oxidase Activity in Nox1-Nox2-Nox4 Triple Knockout Mice: What Do NADPH-Stimulated Chemiluminescence Assays Really Detect? Antioxidants & redox signaling 47 25906178
2005 Alternative mRNA splice forms of NOXO1: differential tissue expression and regulation of Nox1 and Nox3. Gene 46 15949904
2019 Nox1/Ref-1-mediated activation of CREB promotes Gremlin1-driven endothelial cell proliferation and migration. Redox biology 43 30802716
2007 Overexpression of human NOX1 complex induces genome instability in mammalian cells. Free radical biology & medicine 43 17963706
2017 Vascular dysfunction and fibrosis in stroke-prone spontaneously hypertensive rats: The aldosterone-mineralocorticoid receptor-Nox1 axis. Life sciences 42 28478264
2013 Nox1 causes ileocolitis in mice deficient in glutathione peroxidase-1 and -2. Free radical biology & medicine 42 24374371
2014 Nicotine mediates oxidative stress and apoptosis through cross talk between NOX1 and Bcl-2 in lung epithelial cells. Free radical biology & medicine 41 25151121
2007 Nox1 expression determines cellular reactive oxygen and modulates c-fos-induced growth factor, interleukin-8, and Cav-1. The American journal of pathology 39 18055552
2006 NADPH oxidase subunits (NOX-1, p22phox, Rac-1) and tacrolimus-induced nephrotoxicity in a rat renal transplant model. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 38 17085464
2022 Dioscin alleviates myocardial infarction injury via regulating BMP4/NOX1-mediated oxidative stress and inflammation. Phytomedicine : international journal of phytotherapy and phytopharmacology 37 35675750
2021 An interplay of NOX1-derived ROS and oxygen determines the spermatogonial stem cell self-renewal efficiency under hypoxia. Genes & development 36 33446567
2021 Toll-Like Receptor 2 (TLR2) Knockout Abrogates Diabetic and Obese Phenotypes While Restoring Endothelial Function via Inhibition of NOX1. Diabetes 36 34127487
2020 NOX1 Inhibition Attenuates Kidney Ischemia-Reperfusion Injury via Inhibition of ROS-Mediated ERK Signaling. International journal of molecular sciences 36 32967113
2004 Analysis of mRNA transcripts from the NAD(P)H oxidase 1 (Nox1) gene. Evidence against production of the NADPH oxidase homolog-1 short (NOH-1S) transcript variant. The Journal of biological chemistry 36 15375166
2021 Endothelium-restricted endothelin-1 overexpression in type 1 diabetes worsens atherosclerosis and immune cell infiltration via NOX1. Cardiovascular research 33 32533834
2019 NOX1-Dependent mTORC1 Activation via S100A9 Oxidation in Cancer Stem-like Cells Leads to Colon Cancer Progression. Cell reports 33 31365870
2017 Antioxidants and NOX1/NOX4 inhibition blocks TGFβ1-induced CCN2 and α-SMA expression in dermal and gingival fibroblasts. PloS one 33 29049376
2019 Redox Activation of Nox1 (NADPH Oxidase 1) Involves an Intermolecular Disulfide Bond Between Protein Disulfide Isomerase and p47phox in Vascular Smooth Muscle Cells. Arteriosclerosis, thrombosis, and vascular biology 32 30580571
2021 Platelet-derived extracellular vesicles express NADPH oxidase-1 (Nox-1), generate superoxide and modulate platelet function. Free radical biology & medicine 31 33548451
2007 Regulation of NOX1 expression by GATA, HNF-1alpha, and Cdx transcription factors. Free radical biology & medicine 31 18005670
2016 Nox1 promotes colon cancer cell metastasis via activation of the ADAM17 pathway. European review for medical and pharmacological sciences 30 27874952
2014 Phosphorylation of Nox1 regulates association with NoxA1 activation domain. Circulation research 30 25228390
2021 Forestalling age-impaired angiogenesis and blood flow by targeting NOX: Interplay of NOX1, IL-6, and SASP in propagating cell senescence. Proceedings of the National Academy of Sciences of the United States of America 29 34654740
2020 HIV Protease Inhibitor Ritonavir Impairs Endothelial Function Via Reduction in Adipose Mass and Endothelial Leptin Receptor-Dependent Increases in NADPH Oxidase 1 (Nox1), C-C Chemokine Receptor Type 5 (CCR5), and Inflammation. Journal of the American Heart Association 29 33003981
2017 CD47 and Nox1 Mediate Dynamic Fluid-Phase Macropinocytosis of Native LDL. Antioxidants & redox signaling 28 27958762
2015 NOX1 mediates chemoresistance via HIF1α/MDR1 pathway in gallbladder cancer. Biochemical and biophysical research communications 28 26545779
2021 NOX1 inhibition attenuates the development of a pro-tumorigenic environment in experimental hepatocellular carcinoma. Journal of experimental & clinical cancer research : CR 27 33485364
2021 Chitosan inhibits septin-mediated plant infection by the rice blast fungus Magnaporthe oryzae in a protein kinase C and Nox1 NADPH oxidase-dependent manner. The New phytologist 27 33570748
2024 NOX1 triggers ferroptosis and ferritinophagy, contributes to Parkinson's disease. Free radical biology & medicine 26 38876456
2020 Nox1/4 inhibition exacerbates age dependent perivascular inflammation and fibrosis in a model of spontaneous hypertension. Pharmacological research 26 33131726
2016 NOX1/2 activation in human gingival fibroblasts by Fusobacterium nucleatum facilitates attachment of Porphyromonas gingivalis. Archives of microbiology 26 27071620
2022 Renal NOXA1/NOX1 Signaling Regulates Epithelial Sodium Channel and Sodium Retention in Angiotensin II-induced Hypertension. Antioxidants & redox signaling 25 34714114
2022 Setanaxib (GKT137831) Ameliorates Doxorubicin-Induced Cardiotoxicity by Inhibiting the NOX1/NOX4/Reactive Oxygen Species/MAPK Pathway. Frontiers in pharmacology 25 35444554
2023 BRD9 Inhibition Attenuates Matrix Degradation and Pyroptosis in Nucleus Pulposus by Modulating the NOX1/ROS/NF-κB axis. Inflammation 23 36801999
2011 NOX1 participates in ROS-dependent cell death of colon epithelial Caco2 cells induced by Entamoeba histolytica. Microbes and infection 23 21723410
2019 Nox1 downregulators: A new class of therapeutics. Steroids 22 31518594
2015 Protein disulfide isomerase expression increases in resistance arteries during hypertension development. Effects on Nox1 NADPH oxidase signaling. Frontiers in chemistry 22 25870854
2020 NOX1/NADPH oxidase affects the development of autism-like behaviors in a maternal immune activation model. Biochemical and biophysical research communications 21 33310189
2011 RGS2 is a negative regulator of STAT3-mediated Nox1 expression. Cellular signalling 21 22120521

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