Affinage

NMT1

Glycylpeptide N-tetradecanoyltransferase 1 · UniProt P30419

Round 2 corrected
Length
496 aa
Mass
56.8 kDa
Annotated
2026-04-29
73 papers in source corpus 22 papers cited in narrative 23 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NMT1 is a glycylpeptide N-tetradecanoyltransferase that catalyzes the cotranslational and posttranslational transfer of myristate from myristoyl-CoA to the N-terminal glycine α-amino group of substrate proteins, thereby governing their membrane association, subcellular trafficking, stability, and signaling competence. Its substrates span G-protein α-subunits, Src-family kinases, HIV Gag/Nef/matrix, BID, CHP1, VILIP3, ICAM-1, and TMEM106B; myristoylation of BID after caspase-8 cleavage redirects truncated BID to mitochondria to promote cytochrome c release and apoptosis (PMID:11099414), while myristoylation of CHP1 facilitates PD-L1 plasma-membrane translocation and immune evasion (PMID:40605065). NMT1 additionally catalyzes a less efficient lysine ε-amino (K-myristoylation) reaction using a direct catalytic-base contact distinct from the water-mediated mechanism of canonical G-myristoylation (PMID:36181773). Its enzymatic activity is negatively regulated by Sorbs2 binding upon TNF-α stimulation (PMID:38148048) and is pharmacologically targetable by small-molecule inhibitors including desloratadine (binding Asn-246) and PCLX-001 (PMID:36617552, PMID:40605065).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1985 High

    The identification of N-terminal myristate on p60src established that NMT-catalyzed myristoylation is a defined covalent modification of signaling kinases and retroviral transforming proteins, opening the field of protein myristoylation.

    Evidence Tryptic peptide sequencing and metabolic [3H]myristate labeling of p60src from Rous sarcoma virus

    PMID:3917576

    Open questions at the time
    • NMT enzyme not yet purified or cloned
    • substrate scope unknown
  2. 1990 High

    Mutagenesis of the N-terminal glycine of HIV-1 Gag and Gα subunits demonstrated that NMT-mediated myristoylation is required for stable membrane association, viral assembly, and G-protein signaling, establishing myristoylation as a functional membrane-targeting signal rather than an inert modification.

    Evidence G2A mutagenesis of HIV-1 Gag and Gαo; metabolic labeling; subcellular fractionation and virion assembly assays in mammalian cells

    PMID:2153964 PMID:2405382 PMID:2788277

    Open questions at the time
    • Relative contribution of myristate vs. adjacent basic residues not yet dissected
    • no crystal structure of NMT–substrate complex
  3. 1992 High

    Deletion and chimera mapping of yeast Nmt1p defined the minimal catalytic domain (Ile59–Leu455) and revealed that the N-terminal 59 residues play a non-catalytic role in accessing myristoyl-CoA pools, establishing the domain architecture conserved in human NMT1.

    Evidence Deletion mutagenesis, E. coli reconstitution, yeast nmt1-null complementation, and human/yeast chimeric enzymes

    PMID:1429724

    Open questions at the time
    • Human NMT1 domain boundaries not directly mapped
    • structural basis of substrate specificity determinants unresolved
  4. 1994 High

    Quantitative in vitro membrane-binding studies showed that NMT-added myristate cooperates with adjacent polybasic sequences to form a bipartite membrane-targeting signal on Src and HIV Gag, explaining how a single 14-carbon acyl chain achieves high-affinity membrane anchoring.

    Evidence Chimera constructs, mutagenesis of basic residues, and liposome-binding assays with quantified Kd values for p60c-src and HIV-1 Gag

    PMID:7527558 PMID:8139035

    Open questions at the time
    • Myristyl-switch mechanism not yet characterized
    • contribution of palmitoylation or other secondary signals unclear
  5. 2000 High

    The discovery that caspase-8-cleaved BID undergoes posttranslational myristoylation by NMT, which redirects tBID to mitochondria and promotes cytochrome c release, demonstrated that NMT activity is not limited to cotranslational modification and directly controls apoptotic signaling.

    Evidence In vitro myristoylation, liposome binding, and mitochondrial cytochrome c release assays with glycine-mutant controls

    PMID:11099414

    Open questions at the time
    • Relative contributions of NMT1 vs. NMT2 to posttranslational BID myristoylation not separated
    • in vivo apoptotic relevance not fully dissected
  6. 2003 High

    NMR studies of HIV-1 myristoylated matrix protein revealed an entropic myristyl switch coupled to protein trimerization, showing how NMT-installed myristate is regulated downstream of the transferase reaction to control membrane targeting in a conformationally gated manner.

    Evidence NMR structure determination and analytical ultracentrifugation of myr-MA monomer–trimer equilibrium

    PMID:14699046

    Open questions at the time
    • Switch mechanism not demonstrated for non-viral NMT substrates
    • kinetic regulation of switch in the virion assembly context unclear
  7. 2018 Medium

    NMT1 knockdown in breast cancer cells established a feedback loop where reduced myristoylation triggers ROS accumulation and ER stress, activating JNK-mediated autophagy that suppresses tumor growth, placing NMT1 as a regulatory node integrating lipid modification with stress-response signaling.

    Evidence shRNA knockdown of NMT1; ROS, ER stress, and JNK pathway analysis; in vivo xenograft models

    PMID:30446635

    Open questions at the time
    • Specific substrates mediating ROS/ER stress not identified
    • NMT2 compensation not assessed
    • single lab
  8. 2021 Medium

    Liver-specific NMT1 knockout mice revealed that myristoylation differentially controls protein stability: NDP-class substrates are destabilized via HIST1H4H-mediated ubiquitination, while NUP-class substrates are stabilized because myristoylation prevents their ubiquitination, establishing that NMT1 modulates the ubiquitin–proteasome system.

    Evidence Conditional liver-specific NMT1 KO; click chemistry; iTRAQ proteomics; co-IP and ubiquitination assays

    PMID:34136404

    Open questions at the time
    • Mechanism by which myristoylation recruits or blocks ubiquitin ligases is unclear
    • relevance of POTEE as co-factor needs independent validation
  9. 2022 High

    Structural and kinetic analysis demonstrated that NMT catalyzes both efficient G-myristoylation (water-mediated catalytic base) and inefficient K-myristoylation (direct catalytic base contact), revealing a dual-mechanism catalytic architecture and explaining evolutionary depletion of K-myristoylation motifs in humans.

    Evidence X-ray crystallography of NMT–substrate complexes; mass spectrometry; enzyme kinetics; in silico motif analysis

    PMID:36181773

    Open questions at the time
    • Physiological relevance of K-myristoylation in vivo remains limited to few substrates
    • structural basis of substrate selectivity between NMT1 and NMT2 not resolved
  10. 2023 High

    Identification of VILIP3 as an NMT1 substrate whose myristoylation drives NFκB/Bcl-2 signaling in HCC, combined with the discovery that desloratadine inhibits NMT1 by binding Asn-246, provided the first clinically repurposable inhibitor–target pair and a defined NMT1→VILIP3→NFκB signaling axis.

    Evidence DARTS, SPR for target ID; metabolic labeling/MS for substrate ID; NMT1 KD/OE; CDX/PDO/PDX in vivo models

    PMID:36617552

    Open questions at the time
    • Desloratadine selectivity for NMT1 vs. NMT2 not quantified
    • whether Asn-246 binding is allosteric or competitive unclear
  11. 2023 Medium

    NMT1-mediated myristoylation of ICAM-1 stabilizes the adhesion molecule by blocking FBXO4-mediated ubiquitination, linking NMT1 activity to cell adhesion and tumor cell migration suppression.

    Evidence NMT1 knockdown; co-IP; ubiquitination and half-life assays; wound-healing and Transwell migration

    PMID:37269961

    Open questions at the time
    • ICAM-1 N-terminal myristoylation site not structurally confirmed
    • single-lab finding without independent replication
  12. 2024 Medium

    Sorbs2 was identified as a novel NMT1-binding partner whose association upon TNF-α stimulation paradoxically inhibits NMT1 catalytic activity despite increased NMT1 expression, revealing a post-translational regulatory mechanism for NMT1 in osteoblasts.

    Evidence IP/MS identification of Sorbs2; click-it myristoylation assay; RNAi in MC3T3-E1 osteoblastic cells

    PMID:38148048

    Open questions at the time
    • Binding interface between Sorbs2 and NMT1 not mapped
    • functional consequence for osteoblast biology not fully defined
    • single lab, single cell type
  13. 2025 High

    NMT1 myristoylates both Gly2 and Lys3 of TMEM106B, directing it to lysosomes for degradation; non-myristoylated TMEM106B accumulates at the cell surface, establishing dual G- and K-myristoylation as a physiological trafficking determinant for a single substrate.

    Evidence Gly2/Lys3 mutagenesis; NMT1/2 overexpression and knockdown; live-cell imaging; lysosomal inhibitor rescue

    PMID:40451428

    Open questions at the time
    • Relative contribution of NMT1 vs. NMT2 to TMEM106B modification not separated
    • structural basis for dual-site myristoylation unclear
  14. 2025 Medium

    HIF1α-driven NMT1 upregulation mediates CHP1 myristoylation, which facilitates PD-L1 membrane translocation and immune evasion in HNSCC, and pharmacological NMT1 inhibition by PCLX-001 enhances anti-PD-1 immunotherapy efficacy in vivo.

    Evidence HIF1α manipulation; click chemistry; co-IP of CHP1–PD-L1; membrane fractionation; xenograft mouse model with anti-PD-1 + PCLX-001

    PMID:40605065

    Open questions at the time
    • CHP1–PD-L1 binding interface not structurally characterized
    • whether PCLX-001 effects are NMT1-specific or also involve NMT2 not distinguished

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the full substrate repertoire that distinguishes NMT1 from NMT2, the structural basis for NMT1 substrate selectivity, the physiological scope of K-myristoylation, and whether NMT1-selective (vs. pan-NMT) inhibitors can be developed for therapeutic use.
  • NMT1 vs. NMT2 substrate discrimination rules not established
  • no high-resolution human NMT1 structure with endogenous substrates
  • in vivo essentiality of NMT1 in adult tissues not comprehensively characterized

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016740 transferase activity 9
Localization
GO:0005829 cytosol 2 GO:0005886 plasma membrane 1
Pathway
R-HSA-392499 Metabolism of proteins 6 R-HSA-162582 Signal Transduction 3 R-HSA-168256 Immune System 3 R-HSA-1430728 Metabolism 2 R-HSA-5357801 Programmed Cell Death 1
Partners
BIDCHP1ICAM-1SORBS2TMEM106BVILIP3

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1985 The transforming protein p60src of Rous sarcoma virus is N-terminally myristoylated at its amino-terminal glycine residue, establishing that myristoylation of N-terminal glycine is a posttranslational modification catalyzed by NMT on signaling kinases and retroviral transforming proteins. Isolation and sequencing of tryptic peptide from p60src; metabolic labeling with myristic acid Science High 3917576
1989 Myristoylation of the N-terminal glycine of HIV-1 Gag precursor Pr55gag, catalyzed by cellular NMT, is essential for virus particle assembly and budding; G2A mutation abolishing myristoylation prevents virus particle release. Site-directed mutagenesis of Gag glycine-2; metabolic labeling; virion morphogenesis assay Proceedings of the National Academy of Sciences of the United States of America High 2788277
1990 Myristoylation of G-protein alpha subunits (Gi1, Gi2, Gi3, Go, Gt, Gz but not Gs) by cellular NMT is required for their stable membrane association; mutation of the N-terminal glycine to alanine in Gαo abolishes both myristoylation and membrane binding. Metabolic [3H]myristate labeling of COS cell transfectants; site-directed mutagenesis; subcellular fractionation Proceedings of the National Academy of Sciences of the United States of America High 2153964
1990 Myristoylation of HIV-1 Pr55gag by cellular NMT is required for stable membrane association and virus assembly; non-myristoylated Gag accumulates in cells but is not incorporated into virions, though it can still be cleaved by HIV protease in vitro, demonstrating myristate is not required for protease recognition. G-to-A mutagenesis of Gag; metabolic labeling; complementation in trans; in vitro protease cleavage assay Proceedings of the National Academy of Sciences of the United States of America High 2405382
1992 The minimal catalytic domain of S. cerevisiae Nmt1p lies between residues Ile59–Phe96 (N-terminal boundary) and Gly451–Leu455 (C-terminal boundary); the N-terminal 59 residues play a non-catalytic targeting role enabling access to myristoyl-CoA pools from exogenous fatty acid. Peptide substrate specificity differences between yeast Nmt1p and human NMT involve elements distributed across both halves of the enzyme. Deletion mutagenesis; co-expression with substrates in E. coli; in vitro enzyme assay; yeast complementation of nmt1 null allele; human/yeast chimeric enzymes The Journal of biological chemistry High 1429724
1994 The membrane-targeting domain of HIV-1 Gag consists of a bipartite signal: the myristoylated N-terminal 14 amino acids (added by NMT) and an adjacent highly basic region that binds acidic phospholipids. Both myristate and the basic region are required for efficient membrane binding and virion formation. Gag-Src chimera constructs; in vitro membrane binding assay; mutagenesis of basic residues; virion formation assay in vivo Journal of virology High 8139035
1994 Membrane binding of pp60c-src requires both NMT-mediated N-terminal myristoylation and six basic residues that interact electrostatically with acidic phospholipids; removal of either element drastically reduces membrane affinity (Kd shifts from ~0.6 µM to ~2 mM for neutral bilayers). In vitro lipid vesicle binding assay; mutagenesis of basic residues; metabolic labeling; transformation assay Proceedings of the National Academy of Sciences of the United States of America High 7527558
1998 Cryptococcus neoformans NMT is an essential enzyme whose inhibition causes under-myristoylation of ADP ribosylation factor (Arf) and cell death; this fungicidal effect of a depeptidized NMT inhibitor is NMT-dependent, as demonstrated using isogenic strains expressing either fungal or human NMT. Homologous recombination to introduce conditional lethal nmt allele; strain-specific inhibitor sensitivity assay; kinetic studies of purified fungal vs. human NMT; protein synthesis inhibition assay The Journal of biological chemistry High 9575206
1999 NMT is targeted to membranes in mammary epithelial cells via interaction with ribosomal proteins, and its subcellular localization is an important variable in the regulation of cellular proliferation; NMT expression and membrane/cytosol distribution are differentially regulated during normal mammary gland development and in breast cancer transformation. Subcellular fractionation; expression analysis during pregnancy/lactation and malignant transformation; comparison of normal vs. cancerous human breast tissue Advances in enzyme regulation Medium 10470373
2000 BID undergoes posttranslational (non-cotranslational) N-myristoylation by NMT after caspase-8 cleavage exposes a glycine residue; this myristoylation enables BID fragments to target mitochondria and enhances cytochrome c release and apoptosis, functioning as a molecular activating switch. In vitro myristoylation assay; liposome binding assay with defined lipid compositions; mitochondria targeting assay; cytochrome c release assay; mutagenesis of glycine acceptor Science High 11099414
2000 CaBP1 and CaBP2, novel Ca²⁺-binding proteins expressed in the retina, contain consensus sequences for N-terminal myristoylation and are fatty acid acylated in vitro by NMT; their subcellular localization patterns depend on whether they are myristoylated. In vitro myristoylation assay; heterologous expression with subcellular localization analysis The Journal of biological chemistry Medium 10625670
2001 HIV-1 Nef is N-terminally myristoylated by cellular NMT; this myristoylation is required for membrane association of Nef and is critical for Nef-mediated CD4 downregulation and viral pathogenesis. Structure-function analysis; mutagenesis of N-terminal glycine; metabolic labeling; CD4 downregulation assays EMBO reports High 11463741
2003 The HIV-1 matrix protein (myr-MA) adopts myristate-exposed and myristate-sequestered states that are in equilibrium; myristate exposure is coupled to trimerization (not mechanically induced conformational change), constituting an entropic myristyl switch. This switch regulates Gag targeting to the plasma membrane during assembly. NMR structural studies of myr-MA in both states; sedimentation equilibrium analytical ultracentrifugation; equilibrium constant measurements for monomer-trimer transition Proceedings of the National Academy of Sciences of the United States of America High 14699046
2018 Genetic inhibition of NMT1 in breast cancer cells promotes ROS accumulation and ER stress, which cross-talk to produce more ROS. Both oxidative stress and ER stress activate the JNK pathway, leading to autophagy that suppresses breast cancer progression. ROS negatively regulate NMT1 expression, forming a feedback loop. shRNA knockdown of NMT1; ROS measurement; ER stress markers; JNK pathway analysis; autophagy assays; in vitro and in vivo xenograft models Cell death & disease Medium 30446635
2021 NMT1-mediated N-myristoylation differentially regulates two protein classes in liver cancer: NDP proteins (LXN, RPL29, FAU) are destabilized by myristoylation via HIST1H4H-mediated ubiquitination facilitated by RPL7A, while NUP proteins (AHSG, ALB, TF) are stabilized by myristoylation because HBB prevents their ubiquitination by HIST1H4H. Both effects depend on NMT1 and POTEE. Conditional liver-specific NMT1 knockout mice; click chemistry N-myristoylation assay; iTRAQ proteomics; co-immunoprecipitation; ubiquitination assays Frontiers in oncology Medium 34136404
2022 NMT catalyzes both G-myristoylation (N-terminal glycine α-amino group, efficient) and K-myristoylation (lysine ε-amino group, poor efficiency); G-myristoylation uses a water-mediated interaction with the NMT catalytic base while K-myristoylation uses direct interaction. Evolutionary pressure has depleted K-myristoylation motifs in humans. K-myristoylation likely only results from post-translational events. Mass spectrometry; enzyme kinetics; in silico analysis; X-ray crystallography of NMT complexes Journal of molecular biology High 36181773
2023 NMT1 myristoylates VILIP3 at its N-terminus; desloratadine binds Asn-246 in NMT1 and inhibits its enzymatic activity, blocking VILIP3 myristoylation and suppressing downstream NFκB/Bcl-2 signaling to reduce HCC tumor growth. DARTS and SPR assays to identify NMT1 as desloratadine target; metabolic labeling and mass spectrometry to identify VILIP3 as NMT1 substrate; NMT1 knockdown/overexpression; CDX/PDO/PDX models Signal transduction and targeted therapy High 36617552
2023 NMT1 N-myristoylates the N-terminus of ICAM-1 and prevents its ubiquitination by inhibiting the Ub E3 ligase FBXO4 (F-box protein 4), thereby stabilizing ICAM-1 and sustaining cell adhesion while suppressing tumor cell migration. NMT1 knockdown; co-immunoprecipitation; ubiquitination assay; protein half-life measurement; wound healing and Transwell migration assay Cellular signalling Medium 37269961
2024 TNF-α stimulation of osteoblasts increases NMT1 expression but paradoxically inhibits overall protein myristoylation; Sorbs2 was identified as a novel NMT1 binding protein by immunoprecipitation/mass spectrometry, and TNF-α-induced Sorbs2–NMT1 binding inhibits myristoylation activity. Click-it myristoylation assay; RNA interference; immunoprecipitation/mass spectrometry; immunocytochemistry; western blotting in MC3T3-E1 osteoblastic cells In vivo Medium 38148048
2024 NMT1 and NMT2 myristoylate the mammarenavirus matrix Z protein and stable signal peptide (SSP) at their N-terminal glycines; pharmacological NMT inhibition with DDD85464 reduces Z-mediated budding, impairs GP2-mediated fusion activity, and promotes proteasomal degradation of Z protein, exerting potent antiviral activity against LCMV, JUNV, and LASV. NMT inhibitor DDD85464 treatment; Z budding assay; GP2 fusion assay; proteasome inhibition rescue; antiviral titer assays for multiple arenaviruses bioRxivpreprint Medium
2025 Hypoxia-inducible factor HIF1α upregulates NMT1, which mediates myristoylation of calcineurin B homologous protein 1 (CHP1); myristoylated CHP1 binds PD-L1 and facilitates its rapid translocation to the cell membrane, enhancing immune evasion. NMT1 inhibitor PCLX-001 blocks CHP1 myristoylation and disrupts excessive PD-L1 membrane localization. HIF1α overexpression/knockdown; NMT1 knockdown; click chemistry myristoylation assay; co-immunoprecipitation of CHP1 and PD-L1; membrane fractionation; HNSCC xenograft mouse model with anti-PD-1 + PCLX-001 combination Journal of experimental & clinical cancer research Medium 40605065
2025 TMEM106B is myristoylated by NMT1/2 at both its Gly2 α-amino group (G-myristoylation) and Lys3 ε-amino group (K-myristoylation); myristoylation promotes lysosomal degradation of TMEM106B and regulates its trafficking, as non-myristoylated TMEM106B accumulates at the cell surface rather than lysosomes. Mutagenesis of Gly2 and Lys3; NMT1/2 overexpression and knockdown; subcellular fractionation; live-cell imaging; lysosomal inhibitor experiments The Journal of biological chemistry High 40451428
2025 NMT1-mediated myristoylation in the ischemic brain regulates VPS15 (PI3K regulatory subunit 4) expression: ischemia-induced downregulation of NMT1 increases myristic acid (MA) levels in the penumbra, and elevated MA reduces VPS15 expression to exacerbate ischemia injury. The NMT1/MA/VPS15 pathway operates in the ischemic cerebral cortex. NMT1 knockdown in rat cerebral ischemia-reperfusion model; intraperitoneal MA injection; BCtDCS intervention; VPS15 expression analysis; infarct volume measurement Experimental neurology Medium 40221008

Source papers

Stage 0 corpus · 73 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2006 Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 2861 17081983
2012 Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. Cell 1718 22658674
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2006 A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nature biotechnology 1336 16964243
2008 Identification of host proteins required for HIV infection through a functional genomic screen. Science (New York, N.Y.) 1165 18187620
2004 Large-scale characterization of HeLa cell nuclear phosphoproteins. Proceedings of the National Academy of Sciences of the United States of America 1159 15302935
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
1989 Role of capsid precursor processing and myristoylation in morphogenesis and infectivity of human immunodeficiency virus type 1. Proceedings of the National Academy of Sciences of the United States of America 789 2788277
1990 nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine. The Journal of biological chemistry 752 2358444
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
1993 TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility. Gene 616 8422997
2011 Global landscape of HIV-human protein complexes. Nature 593 22190034
1990 Myristoylation-dependent replication and assembly of human immunodeficiency virus 1. Proceedings of the National Academy of Sciences of the United States of America 569 2405382
1994 Identification of a membrane-binding domain within the amino-terminal region of human immunodeficiency virus type 1 Gag protein which interacts with acidic phospholipids. Journal of virology 484 8139035
2000 Posttranslational N-myristoylation of BID as a molecular switch for targeting mitochondria and apoptosis. Science (New York, N.Y.) 472 11099414
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2015 A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface. Cell 433 26638075
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2001 Structure--function relationships in HIV-1 Nef. EMBO reports 317 11463741
1990 G-protein alpha-subunit expression, myristoylation, and membrane association in COS cells. Proceedings of the National Academy of Sciences of the United States of America 277 2153964
2012 A high-throughput approach for measuring temporal changes in the interactome. Nature methods 273 22863883
2003 Entropic switch regulates myristate exposure in the HIV-1 matrix protein. Proceedings of the National Academy of Sciences of the United States of America 272 14699046
1985 Amino terminal myristylation of the protein kinase p60src, a retroviral transforming protein. Science (New York, N.Y.) 256 3917576
1994 Amino-terminal basic residues of Src mediate membrane binding through electrostatic interaction with acidic phospholipids. Proceedings of the National Academy of Sciences of the United States of America 223 7527558
2000 Five members of a novel Ca(2+)-binding protein (CABP) subfamily with similarity to calmodulin. The Journal of biological chemistry 221 10625670
1992 Comparative analysis of the beta transducin family with identification of several new members including PWP1, a nonessential gene of Saccharomyces cerevisiae that is divergently transcribed from NMT1. Proteins 100 1594577
2003 Prediction of lipid posttranslational modifications and localization signals from protein sequences: big-Pi, NMT and PTS1. Nucleic acids research 73 12824382
2023 Blockade of NMT1 enzymatic activity inhibits N-myristoylation of VILIP3 protein and suppresses liver cancer progression. Signal transduction and targeted therapy 59 36617552
1992 Studies of the catalytic activities and substrate specificities of Saccharomyces cerevisiae myristoyl-coenzyme A: protein N-myristoyltransferase deletion mutants and human/yeast Nmt chimeras in Escherichia coli and S. cerevisiae. The Journal of biological chemistry 49 1429724
2021 Plasticity in Neuroblastoma Cell Identity Defines a Noradrenergic-to-Mesenchymal Transition (NMT). Cancers 47 34200747
1998 Genetic and biochemical studies establish that the fungicidal effect of a fully depeptidized inhibitor of Cryptococcus neoformans myristoyl-CoA:protein N-myristoyltransferase (Nmt) is Nmt-dependent. The Journal of biological chemistry 37 9575206
2018 NMT1 inhibition modulates breast cancer progression through stress-triggered JNK pathway. Cell death & disease 35 30446635
1987 Characterization of a novel N-methyltransferase (NMT) from Catharanthus roseus plants : Detection of NMT and other enzymes of the indole alkaloid biosynthetic pathway in different cell suspension culture systems. Plant cell reports 31 24248932
1998 Nascent transcription from the nmt1 and nmt2 genes of Schizosaccharomyces pombe overlaps neighbouring genes. The EMBO journal 28 9606189
1996 A radioresistant variant cell line, NMT-1R, isolated from a radiosensitive rat yolk sac tumour cell line, NMT-1: differences of early radiation-induced morphological changes, especially apoptosis. International journal of radiation biology 27 8613682
1994 nmt2 of fission yeast: a second thiamine-repressible gene co-ordinately regulated with nmt1. Yeast (Chichester, England) 22 7992507
1994 ntf1+ encodes a 6-cysteine zinc finger-containing transcription factor that regulates the nmt1 promoter in fission yeast. The Journal of biological chemistry 19 8163491
2018 NMT1 and NMT3 N-Methyltransferase Activity Is Critical to Lipid Homeostasis, Morphogenesis, and Reproduction. Plant physiology 18 29777000
1993 Establishment and characterization of a rat yolk sac tumor cell line, NMT-1, producing alpha-fetoprotein, with potential for lymphatic metastasis. Japanese journal of cancer research : Gann 15 7507475
2021 N-Myristoylation by NMT1 Is POTEE-Dependent to Stimulate Liver Tumorigenesis via Differentially Regulating Ubiquitination of Targets. Frontiers in oncology 11 34136404
2021 QSAR and Pharmacophore Modeling of Nitrogen Heterocycles as Potent Human N-Myristoyltransferase (Hs-NMT) Inhibitors. Molecules (Basel, Switzerland) 10 33805223
2011 A chemical compound for controlled expression of nmt1-driven gene in the fission yeast Schizosaccharomyces pombe. Analytical biochemistry 10 21295003
2003 Characterization and expression of the Neurospora crassa nmt-1 gene. Current genetics 10 13680155
2022 Structural and Large-scale Analysis Unveil the Intertwined Paths Promoting NMT-catalyzed Lysine and Glycine Myristoylation. Journal of molecular biology 9 36181773
1999 Expression of enzymes of covalent protein modification during regulated and dysregulated proliferation of mammary epithelial cells: PKA, PKC and NMT. Advances in enzyme regulation 9 10470373
2004 Molecular characterization of a gene encoding N-myristoyl transferase (NMT) from Triticum aestivum (bread wheat). Genome 8 15644961
2023 NMT1 sustains ICAM-1 to modulate adhesion and migration of tumor cells. Cellular signalling 7 37269961
2015 An IPTG-inducible derivative of the fission yeast nmt promoter. Yeast (Chichester, England) 7 25801050
2021 Long noncoding RNA ANCR promotes migration, invasion, EMT progress and stemness of nasopharyngeal carcinoma cells via the miR-4731-5p/NMT1 axis. Pathology, research and practice 6 34333213
2019 Regulation of the thiamine pyrophosphate (TPP)-sensing riboswitch in NMT1 mRNA from Neurospora crassa. FEBS letters 6 31664711
1995 An 8.2 kb DNA segment from chromosome XIV carries the RPD3 and PAS8 genes as well as the Saccharomyces cerevisiae homologue of the thiamine-repressed nmt1 gene and a chromosome III-duplicated gene for a putative aryl-alcohol dehydrogenase. Yeast (Chichester, England) 6 8533474
2022 Identification of potential inhibitors for N-myristoyltransferase (NMT) protein of Plasmodium vivax. Journal of biomolecular structure & dynamics 5 36002266
2006 Joint regulation of the nmt1 promoter and sporulation by Thi1 and Thi5 in Schizosaccharomyces pombe. Current genetics 5 16874521
2023 Mechanism of Ligand Discrimination by the NMT1 Riboswitch. Journal of chemical information and modeling 4 37486304
2022 NMT1 Enhances the Stemness of NSCLC Cells by Activating the PI3K/AKT Pathway. Pharmacology 3 35732157
2025 Elucidating the role of N-myristoylation in the excessive membrane localization of PD-L1 in hypoxic cancers and developing a novel NMT1 inhibitor for combination with immune checkpoint blockade therapy. Journal of experimental & clinical cancer research : CR 2 40605065
2021 The role of NMT induction on odontogenic proliferation and differentiation of dental pulp stem cells. Heliyon 2 33937538
2025 Myristoylation of TMEM106B by NMT1/2 regulates TMEM106B trafficking and turnover. The Journal of biological chemistry 1 40451428
2025 Molecular docking and simulation analysis of selected herbal compounds against GP63, FPPS, and NMT, three important Leishmania major proteins. Research in pharmaceutical sciences 1 40933604
2024 TNF-α-induced Inhibition of Protein Myristoylation Via Binding Between NMT1 and Sorbs2 in Osteoblasts. In vivo (Athens, Greece) 1 38148048
2024 Development of a genetically encoded NMT indicator for detection of mercury ions based on the green fluorescent protein mNeonGreen and metallothionein II from rat liver. Heliyon 1 38975119
2022 Inspection of in-house designed novel thiochromone amino-acid conjugate derivatives as Lm-NMT inhibitor - An in-silico analysis. Journal of molecular graphics & modelling 1 36542915
2019 N-Myristoyl Transferase (NMT)-Catalyzed Labeling of Bacterial Proteins for Imaging in Fixed and Live Cells. Methods in molecular biology (Clifton, N.J.) 1 31161515
2025 Identification of NMT1/MA/VPS15 signal pathway as potential therapeutic target in rat cerebral ischemia injury. Experimental neurology 0 40221008
2025 Mitigating cadmium toxicity in rice through tandem application of zinc oxide nanoparticles and Serendipita indica as revealed by multi-omics and NMT-based ion flux analysis. Journal of hazardous materials 0 41406521
2014 The crystal structure of pyrimidine/thiamin biosynthesis precursor-like domain-containing protein CAE31940 from proteobacterium Bordetella bronchiseptica RB50, and evolutionary insight into the NMT1/THI5 family. Journal of structural and functional genomics 0 24908050