Affinage

NEK9

Serine/threonine-protein kinase Nek9 · UniProt Q8TD19

Length
979 aa
Mass
107.2 kDa
Annotated
2026-06-10
37 papers in source corpus 24 papers cited in narrative 24 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/8 claims corpus-supported (88%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NEK9 (Nercc1) is a NIMA-family serine/threonine kinase that serves as the master upstream node of a mitotic kinase cascade controlling centrosome maturation and spindle assembly (PMID:12840024, PMID:16079175). It is a cytoplasmic kinase activated during mitosis, binding the Ran GTPase through both its catalytic and RCC1-like domains and self-oligomerizing for autoactivation via activation-loop phosphorylation at Thr210 (PMID:12101123, PMID:16079175). In prophase NEK9 is switched on by sequential CDK1 then Plk1 phosphorylation, after which it phosphorylates and activates the downstream kinases Nek6 and Nek7 by binding their kinase domains through its non-catalytic C-terminal domain and releasing an autoinhibitory tyrosine (Tyr97) via back-to-back dimerization (PMID:12840024, PMID:19941817, PMID:21642957, PMID:26522158). Through this module NEK9 drives prophase centrosome separation: it promotes Nek6/7-dependent phosphorylation of the kinesin Eg5 at Ser1033 and phosphorylates TPX2 on its nuclear localization signal to retain a centrosomal TPX2 pool that organizes microtubule asters (PMID:21642957, PMID:29276125). Independently of Nek6/7, NEK9 phosphorylates NEDD1/GCP-WD at Ser377 to recruit gamma-tubulin and mature the centrosome (PMID:22818914). Signal flux through the cascade is gated by the dynein light chain DYNLL/LC8, which binds a motif near the NEK9 coiled-coil to promote multimerization while competing with Nek6 binding, a interaction abolished by NEK9 autophosphorylation at Ser944 (PMID:21454704, PMID:23482567). Beyond mitosis, NEK9 acts as a selective autophagy adaptor that targets MYH9 (myosin IIA) for degradation via its LIR motif to enable primary ciliogenesis (PMID:34078910), promotes CHK1 activity during replication stress (PMID:25217585), and phosphorylates substrates including ARHGEF2 to activate RhoA and Raptor at Ser792 to inhibit mTORC1 (PMID:33500736, PMID:39627360). Activating mutations in the kinase and RCC1 domains, which elevate Thr210 phosphorylation, cause the cutaneous disorder nevus comedonicus by disrupting follicular differentiation (PMID:27153399).

Mechanistic history

Synthesis pass · year-by-year structured walk · 22 steps
  1. 2002 High

    Establishing NEK9 as a Ran-binding, mitotically activated kinase defined its identity as a cytoplasmic cell-cycle kinase and first linked it to the Ran GTPase and Nek6.

    Evidence Co-IP, subcellular fractionation, and in vitro autoactivation assays in the founding paper

    PMID:12101123

    Open questions at the time
    • Functional consequence of RanGDP binding not resolved
    • No downstream substrates yet identified
  2. 2003 High

    Identifying Nek6/Nek7 as direct activation-loop substrates established NEK9 as the apex of a mitotic NIMA-family kinase cascade required for spindle integrity.

    Evidence In vitro kinase assays with recombinant proteins, phosphosite mapping (Ser206), and antibody microinjection

    PMID:12840024

    Open questions at the time
    • Structural basis of substrate recognition unknown at this stage
    • Upstream activator of NEK9 itself not yet defined
  3. 2003 Medium

    Discovery of a stable NEK9-FACT complex extended NEK9 function beyond mitosis into interphase G1/S progression.

    Evidence Co-IP of ~600 kDa complex with Spt16-Pob3 and RNAi cell-cycle analysis

    PMID:14660563

    Open questions at the time
    • FACT substrate/phosphorylation target within complex not defined
    • Single lab, no reciprocal validation
  4. 2005 High

    Localizing active NEK9 to centrosomes and showing rescue of spindle defects established a direct role in spindle assembly and gamma-TuRC association.

    Evidence Immunodepletion from Xenopus egg extracts with recombinant rescue and gamma-tubulin co-IP

    PMID:16079175

    Open questions at the time
    • Direct gamma-TuRC substrate not yet identified
    • Mechanism of centrosomal targeting unresolved
  5. 2009 High

    The Nek7 autoinhibited structure and the role of the NEK9 CTD in releasing Tyr97 revealed the molecular mechanism of downstream kinase activation.

    Evidence X-ray crystallography of Nek7 plus Tyr-to-Phe mutagenesis and activity assays

    PMID:19941817

    Open questions at the time
    • Exact stoichiometry and dimerization geometry not resolved here
    • How CTD engagement is regulated in cells unclear
  6. 2011 High

    Defining sequential CDK1-then-Plk1 activation of NEK9 and the NEK9-Nek6/7-Eg5 axis placed the cascade in the prophase centrosome-separation program.

    Evidence In vitro kinase reconstitution, phosphosite mapping (Eg5 Ser1033), siRNA epistasis, and live imaging

    PMID:21642957

    Open questions at the time
    • Spatiotemporal coordination of the two upstream kinases not fully mapped
    • Other Nek6/7 mitotic substrates unaccounted for
  7. 2011 High

    Showing LC8/DYNLL binding promotes multimerization yet competes with Nek6 revealed an autophosphorylation-gated switch controlling cascade output.

    Evidence Reciprocal co-IP, motif mutagenesis, and in vitro kinase assays defining Ser944 regulation

    PMID:21454704

    Open questions at the time
    • Cellular trigger for Ser944 autophosphorylation timing unknown
    • Whether LC8 links NEK9 to dynein-based transport untested
  8. 2012 High

    Identifying NEDD1 Ser377 as a direct NEK9 substrate explained how NEK9 drives gamma-tubulin recruitment independently of Nek6/7.

    Evidence In vitro kinase assay, phosphosite mutagenesis, and validation in Xenopus and mammalian cells

    PMID:22818914

    Open questions at the time
    • Branch-point logic separating NEDD1 from Nek6/7 arms not fully defined
  9. 2012 Medium

    Oocyte meiosis phenotypes confirmed NEK9 requirement for spindle organization, chromosome alignment, and checkpoint silencing in a physiological division.

    Evidence Morpholino knockdown in mouse oocytes with confocal imaging and checkpoint marker analysis

    PMID:23159858

    Open questions at the time
    • Direct meiotic substrates not identified
    • Single knockdown approach
  10. 2013 High

    Structures of LC8 bound to phospho- and unphospho-Nek9 peptides established the atomic basis for Ser944-dependent affinity regulation of the cascade switch.

    Evidence X-ray crystallography of two peptide states plus ITC

    PMID:23482567

    Open questions at the time
    • In-cell kinetics of the switch not measured
  11. 2014 Medium

    Linking NEK9 to CHK1 activity defined a role in the replication stress response distinct from its mitotic functions.

    Evidence Co-IP, kinase activity assays, RNAi synthetic-lethal screen, and gemcitabine sensitivity

    PMID:25217585

    Open questions at the time
    • Whether NEK9 directly phosphorylates CHK1 not established
    • Single lab
  12. 2015 High

    The Nek7(Y97F)-Nek9 peptide structure showing back-to-back dimerization completed the mechanistic model for how the NEK9 CTD activates downstream kinases.

    Evidence X-ray crystallography, binding-domain mapping (Nek9 810-828), and structure-function mutagenesis

    PMID:26522158

    Open questions at the time
    • Dynamics of dimer assembly in vivo not visualized
  13. 2015 Medium

    Identifying NEK9 as a viral-recruited transcriptional repressor at GADD45A revealed an unexpected chromatin-associated, infection-dependent activity.

    Evidence siRNA knockdown, ChIP, colocalization with viral E4 orf3, and replication assays

    PMID:26676776

    Open questions at the time
    • Mechanism of promoter recruitment unknown
    • Relevance outside viral infection unclear
  14. 2016 Medium

    Discovering activating somatic NEK9 mutations in nevus comedonicus established a direct disease link and showed NEK9 kinase activity regulates follicular differentiation.

    Evidence Whole-exome sequencing with pThr210 Western blot and differentiation-marker immunofluorescence

    PMID:27153399

    Open questions at the time
    • Downstream effectors in keratinocyte differentiation not identified
    • Single study
  15. 2017 High

    Showing NEK9 phosphorylates the TPX2 NLS to block importin binding explained how a centrosomal TPX2 pool is maintained for prophase aster organization.

    Evidence In vitro kinase assay, importin-binding assay, siRNA epistasis with RHAMM/HMMR, and live imaging

    PMID:29276125

    Open questions at the time
    • Coordination with the Eg5 and NEDD1 arms not unified mechanistically
  16. 2020 Medium

    Demonstrating EML4-ALK V3 recruitment of NEK9/NEK7 to microtubules implicated the cascade in oncogenic cell migration independent of ALK catalytic activity.

    Evidence Co-IP, constitutively-active mutants, siRNA epistasis, and live imaging

    PMID:32184261

    Open questions at the time
    • Microtubule substrate of NEK9/NEK7 in this context not defined here
    • Single lab
  17. 2021 High

    Identifying NEK9 as a LIR-motif selective autophagy adaptor for MYH9 required for ciliogenesis revealed a non-mitotic degradative function validated in vivo.

    Evidence Co-IP, LIR mutagenesis, mouse knock-in, and MYH9-depletion rescue of ciliogenesis

    PMID:34078910

    Open questions at the time
    • How NEK9 selects MYH9 cargo signal is unresolved
    • Relationship between kinase activity and adaptor function unclear
  18. 2021 Medium

    Defining ARHGEF2 as a direct NEK9 substrate connected the kinase to RhoA activation and cancer cell motility.

    Evidence In vitro kinase assay, phosphoproteomics, GST pull-down, and migration assays

    PMID:33500736

    Open questions at the time
    • ARHGEF2 phosphosite not specified
    • Single lab cancer model
  19. 2021 Medium

    Linking NEK9 to PRLR-SF and Hippo/TEAD signaling extended its reach to metabolic gene regulation in pancreatic cancer.

    Evidence Co-IP, PLA, ChIP, and promoter luciferase assays

    PMID:33664869

    Open questions at the time
    • Whether the effect requires NEK9 kinase activity not established
    • Single lab
  20. 2023 Medium

    Mapping a NEK9-ROBO1 interaction and TRIM28/cortactin substrates placed NEK9 in SLIT2-ROBO1 signaling and transcriptional regulation of CTTN.

    Evidence Mass spectrometry, co-IP, in vitro kinase assay, and domain-deletion analysis

    PMID:37443302

    Open questions at the time
    • Phosphosites on TRIM28/cortactin not defined here
    • Single lab
  21. 2024 Medium

    Identifying USP19-mediated stabilization and NEK9 phosphorylation of Raptor Ser792 connected NEK9 abundance to mTORC1 inhibition and autophagic cell death.

    Evidence Co-IP, in vitro deubiquitylation and kinase assays defining Lys525 ubiquitination and Ser792 phosphorylation

    PMID:39627360

    Open questions at the time
    • Physiological signals controlling the USP19-NEK9 axis unclear
    • Single lab
  22. 2025 Medium

    Showing NEK7 phosphorylates cortactin in its actin-binding region links the NEK9/NEK7 cascade to actin-driven filopodia and migration.

    Evidence In vitro kinase assay with phospho-mimetic/null mutagenesis and live imaging (preprint)

    PMID:bio_10.1101_2025.08.30.672807

    Open questions at the time
    • Preprint, not yet peer-reviewed
    • In vivo relevance of cortactin phosphorylation untested

Open questions

Synthesis pass · forward-looking unresolved questions
  • How NEK9 selects between its many distinct functional outputs—mitotic cascade, autophagy adaptor, replication stress, mTORC1, and cancer signaling—within a single cell remains unresolved.
  • No unifying model for context-dependent substrate selection
  • Relationship between kinase-dependent and adaptor functions not integrated
  • Whether non-mitotic substrates require the same activation hierarchy unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 6 GO:0016740 transferase activity 3 GO:0003924 GTPase activity 1 GO:0060090 molecular adaptor activity 1 GO:0140110 transcription regulator activity 1
Localization
GO:0005815 microtubule organizing center 3 GO:0005634 nucleus 2 GO:0005829 cytosol 1 GO:0005856 cytoskeleton 1
Pathway
R-HSA-1640170 Cell Cycle 5 R-HSA-162582 Signal Transduction 3 R-HSA-9612973 Autophagy 2 R-HSA-1852241 Organelle biogenesis and maintenance 1 R-HSA-73894 DNA Repair 1
Partners
DYNLL1MYH9NEK6NEK7PLK1RANTPX2USP19
Complex memberships
FACT (Spt16-Pob3)gamma-tubulin ring complex (associated)

Evidence

Reading pass · 24 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2002 Nercc1/NEK9 binds specifically to the Ran GTPase through both its catalytic and RCC1-like domains, preferring RanGDP in vivo. Nercc1 also binds Nek6 and exists as a homooligomer, capable of autoactivation in vitro by autophosphorylation. It is a cytoplasmic protein activated during mitosis and phosphorylated by active p34(Cdc2)/CDK1. Co-immunoprecipitation, in vitro autoactivation assay, subcellular fractionation, in vivo binding experiments Genes & development High 12101123
2003 Nercc1/NEK9 directly phosphorylates and activates Nek6 at Ser206 on its activation loop (and Nek7 similarly) in vitro, resulting in 20–25-fold activation of Nek6 activity. Microinjection of anti-Nercc1 antibodies causes spindle abnormalities and prometaphase arrest or chromosome missegregation, establishing Nercc1/Nek9 as the upstream kinase in a mitotic NIMA-family kinase cascade. In vitro kinase assay with recombinant proteins, co-expression of activated Nercc1 mutant, antibody microinjection, mass spectrometry phosphosite identification The Journal of biological chemistry High 12840024
2003 NEK9 forms a stable ~600 kDa complex with the FACT chromatin-remodeling complex (Spt16-Pob3) in interphase nuclei. Within this complex, NEK9 exhibits elevated phosphorylation at Thr210 (activation loop). RNAi depletion of Nek9 impairs G1 and S phase progression, implicating Nek9-FACT complex in interphase cell cycle progression. Co-immunoprecipitation, dsRNAi knockdown, cell cycle analysis, gel mobility shift assay The Journal of biological chemistry Medium 14660563
2005 Active Nercc1/NEK9 localizes to centrosomes and spindle poles during early mitosis. In Xenopus egg extracts, XNercc co-precipitates with gamma-tubulin ring complex components. Immunodepletion of XNercc causes delayed spindle assembly, fewer bipolar spindles, and smaller Ran-GTP-induced asters; these defects are rescued by adding purified recombinant XNercc. Autoactivation in vitro requires phosphorylation of the activation loop at Thr210. Immunofluorescence, co-immunoprecipitation, immunodepletion from Xenopus egg extracts, recombinant protein rescue, in vitro kinase assay Molecular biology of the cell High 16079175
2007 Adenovirus E1A protein interacts with nuclear NEK9 and causes redistribution of NEK9 from nucleus to cytoplasm. A NEK9 deletion mutant lacking the central RCC1-like domain interacts stably with E1A and accumulates in the nucleus, suggesting the RCC1-like domain is required for the transient nature of the interaction. Co-immunoprecipitation, subcellular fractionation, immunofluorescence, deletion mutagenesis Journal of cellular physiology Medium 17443675
2009 Crystal structure of Nek7 reveals an autoinhibited conformation where Tyr97 protrudes into the active site and blocks the alphaC helix. The non-catalytic C-terminal domain (CTD) of Nek9 binds Nek6/Nek7 and releases this autoinhibitory tyrosine, activating the kinases. Tyr-to-Phe mutation of Nek7 and Nek6 renders them constitutively active independently of Nek9. X-ray crystallography, site-directed mutagenesis, in vitro kinase activity assays Molecular cell High 19941817
2011 PLK1 directly activates NEK9 during prophase via sequential phosphorylation by CDK1 then Plk1. Activated NEK9 in turn phosphorylates Nek6/Nek7, which phosphorylate the mitotic kinesin Eg5 at Ser1033; this Nek6/7-dependent phosphorylation together with the CDK1 site Thr926 promotes Eg5 accumulation at centrosomes and drives prophase centrosome separation. In vitro kinase assay, co-immunoprecipitation, siRNA knockdown, phosphosite mapping, live-cell imaging The EMBO journal High 21642957
2011 DYNLL/LC8 binds Nek9 via a (K/R)XTQT motif adjacent to the Nek9 C-terminal coiled-coil, promotes Nek9 multimerization and accelerates Nek9 autoactivation. LC8 binding is negatively regulated by Nek9 autophosphorylation at Ser944. Importantly, LC8 binding to Nek9 interferes with Nek9-Nek6 interaction and inhibits Nek6 activation, thereby controlling signal transduction through the Nek9/Nek6/7 module. Co-immunoprecipitation, in vitro kinase assay, motif mutagenesis, biophysical binding assays The Journal of biological chemistry High 21454704
2012 NEK9 phosphorylates NEDD1/GCP-WD at Ser377, driving NEDD1 recruitment to the centrosome and thereby promoting gamma-tubulin accumulation at the mitotic centrosome. This function requires Plk1-dependent activation of Nek9 but is independent of downstream Nek6/Nek7. In vitro kinase assay, phosphosite mutagenesis, Xenopus egg extracts, siRNA knockdown, immunofluorescence Current biology : CB High 22818914
2012 Nek9 localizes to spindle poles and the midbody during mouse oocyte meiosis. Morpholino-mediated depletion of Nek9 causes defective spindles, misaligned chromosomes, MI arrest, and failure of polar body extrusion, with concomitant loss of gamma-tubulin at spindle poles and retention of Bub3 at kinetochores. Morpholino knockdown in mouse oocytes, confocal immunofluorescence, live-cell imaging Cell cycle (Georgetown, Tex.) Medium 23159858
2013 Crystal structures of LC8 bound to phosphorylated and unphosphorylated Nek9 peptides (around Ser944) reveal that phosphorylation at Ser944 directly reduces binding affinity to LC8, explaining how Nek9 autophosphorylation controls its interaction with the dynein light chain and thereby regulates downstream Nek6 signaling. X-ray crystallography, isothermal titration calorimetry, biophysical binding assays The Journal of biological chemistry High 23482567
2014 NEK9 complexes with CHK1, and NEK9 depletion impairs CHK1 autophosphorylation and kinase activity in response to replication stress. NEK9-depleted cells show hypersensitivity to gemcitabine, spontaneous DNA damage foci, RPA70 foci accumulation, and impaired recovery from replication arrest, defining NEK9 as a component of the replication stress response that promotes CHK1 activity. siRNA knockdown, co-immunoprecipitation, kinase activity assay, RNAi synthetic lethal screen, immunofluorescence Nucleic acids research Medium 25217585
2015 Crystal structure of Nek7(Y97F) bound to a Nek9 peptide (residues 810–828) maps the minimal Nek9-binding region on the C-lobe of the Nek7 kinase domain. Nek7(Y97F) crystallizes as a back-to-back dimer through N-lobe contacts coupled to the conformation of residue 97. Self-association of the Nek9 CTD is required for Nek7 activation, leading to the model that Nek9-CTD promotes Nek7 back-to-back dimerization to release autoinhibition. X-ray crystallography, binding domain mapping, in vitro kinase assay, structure-function mutagenesis Nature communications High 26522158
2015 NEK9 functions as a transcriptional repressor during adenovirus infection, colocalizing with viral replication centers and viral E4 orf3 protein. NEK9 depletion reduces viral genome replication and associates with promoters of p53 target gene GADD45A, silencing its expression. siRNA knockdown, chromatin immunoprecipitation, immunofluorescence colocalization, viral replication assay Journal of virology Medium 26676776
2016 Somatic gain-of-function mutations in the kinase and RCC1 domains of NEK9 cause nevus comedonicus; each mutation increases phosphorylation at Thr210 (the NEK9 activation-loop marker of kinase activation), leading to loss of follicular differentiation markers and ectopic keratin expression, establishing NEK9 kinase activity as a regulator of follicular differentiation. Whole-exome sequencing, Western blot for pThr210, immunofluorescence for differentiation markers American journal of human genetics Medium 27153399
2017 NEK9 phosphorylates TPX2 on its nuclear localization signal (NLS), preventing importin binding and thereby retaining a pool of TPX2 at centrosomes before nuclear envelope breakdown (NEBD). This centrosomal TPX2 pool, dependent on RHAMM/HMMR and NEK9, is required for microtubule aster organization, Eg5 localization, and prophase centrosome separation. In vitro kinase assay, co-immunoprecipitation, siRNA knockdown, live-cell imaging, importin-binding assay Current biology : CB High 29276125
2020 EML4-ALK variant 3 recruits NEK9 and NEK7 to microtubules via the N-terminal EML4 microtubule-binding region. Constitutively active NEK9 perturbs cell morphology and accelerates migration in a microtubule-dependent manner requiring downstream NEK7 but not ALK activity, establishing NEK9/NEK7 as effectors of EML4-ALK V3-driven microtubule stabilization and cell migration. Co-immunoprecipitation, siRNA knockdown, constitutively-active mutant overexpression, live-cell imaging, pharmacological inhibition Journal of cell science Medium 32184261
2021 NEK9 acts as a selective autophagy adaptor for MYH9 (myosin IIA) via its LC3-interacting region (LIR) motif, interacting with GABARAP-family proteins. NEK9-mediated autophagic degradation of MYH9 is required for primary cilia formation; LIR mutation in NEK9 causes MYH9 accumulation and impaired ciliogenesis both in cells and in mouse kidneys. MYH9 depletion rescues ciliogenesis in NEK9-LIR mutant cells. Co-immunoprecipitation, LIR mutagenesis, mouse knock-in model, knockdown rescue experiments, immunofluorescence, autophagy flux assays Nature communications High 34078910
2021 NEK9 directly interacts with PRLR-SF (short isoform of prolactin receptor) and serves as intermediator between PRLR-SF and Hippo signaling pathway activation, suppressing pentose phosphate pathway gene expression (G6PD, TKT) via TEAD1 in pancreatic cancer cells. Co-immunoprecipitation, proximity ligation assay, chromatin immunoprecipitation, promoter luciferase assay Theranostics Medium 33664869
2021 NEK9 directly phosphorylates ARHGEF2 (a RhoA guanine nucleotide exchange factor), as demonstrated by in vitro kinase assay and phosphoproteomics. This phosphorylation activates RhoA and drives gastric cancer cell motility. NEK9 is transcriptionally upregulated via STAT3 activation (by IL-6) through suppression of miR-520f-3p. In vitro kinase assay, phosphoproteomics, GST pull-down, co-immunoprecipitation, luciferase reporter, ChIP Theranostics Medium 33500736
2023 NEK9 kinase domain directly interacts with the intracellular domain (ICD) of ROBO1, and this interaction is enhanced by SLIT2. NEK9 directly phosphorylates TRIM28 and cortactin (CTTN), with TRIM28 functioning as a transcriptional elongation factor that facilitates CTTN transcription via STAT3 and NF-κB p100. Mass spectrometry, co-immunoprecipitation, in vitro kinase assay, domain deletion analysis Cell death & disease Medium 37443302
2024 USP19 deubiquitylase directly interacts with and stabilizes NEK9 by removing K48-linked polyubiquitin chains at Lys525, preventing its proteasomal degradation. NEK9, in turn, phosphorylates Raptor at Ser792, inhibiting mTORC1 signaling and promoting autophagic cell death in pancreatic cancer cells. Co-immunoprecipitation, in vitro deubiquitylation assay, in vitro kinase assay, siRNA/overexpression functional studies Cell death and differentiation Medium 39627360
2025 NEK7 (downstream of NEK9 in the EML4-ALK V3 pathway) phosphorylates cortactin within its F-actin-binding repeat region (ABR). Phospho-mimetic cortactin mutations promote filopodia-like extensions and increased migration, while phospho-null mutations dissociate cortactin from F-actin. Constitutively active NEK9 or NEK7 produces similar cortactin-dependent morphological changes. In vitro kinase assay, phospho-mimetic/null mutagenesis, siRNA depletion, live-cell imaging, co-localization bioRxivpreprint Medium bio_10.1101_2025.08.30.672807
2025 FAM49B directly interacts with NEK9 and promotes Thr210 phosphorylation (NEK9 activation). In FAM49B-overexpressing colorectal cancer cells, NEK9 knockdown significantly reduces c-Myc expression and c-Myc-Ser62 phosphorylation, placing NEK9 between FAM49B and c-Myc stabilization. Co-immunoprecipitation, siRNA knockdown, Western blot for pThr210 and pSer62-c-Myc, ubiquitination assay BioFactors (Oxford, England) Low 39780509

Source papers

Stage 0 corpus · 37 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2003 A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases. The Journal of biological chemistry 154 12840024
2011 Nek9 is a Plk1-activated kinase that controls early centrosome separation through Nek6/7 and Eg5. The EMBO journal 150 21642957
2002 Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression. Genes & development 122 12101123
2009 An autoinhibitory tyrosine motif in the cell-cycle-regulated Nek7 kinase is released through binding of Nek9. Molecular cell 81 19941817
2012 Nek9 phosphorylation of NEDD1/GCP-WD contributes to Plk1 control of γ-tubulin recruitment to the mitotic centrosome. Current biology : CB 68 22818914
2005 Active Nercc1 protein kinase concentrates at centrosomes early in mitosis and is necessary for proper spindle assembly. Molecular biology of the cell 61 16079175
2017 Nek9 Phosphorylation Defines a New Role for TPX2 in Eg5-Dependent Centrosome Separation before Nuclear Envelope Breakdown. Current biology : CB 52 29276125
2015 Mechanistic basis of Nek7 activation through Nek9 binding and induced dimerization. Nature communications 50 26522158
2018 Proteomic analysis discovers the differential expression of novel proteins and phosphoproteins in meningioma including NEK9, HK2 and SET and deregulation of RNA metabolism. EBioMedicine 49 30594554
2021 NEK9 regulates primary cilia formation by acting as a selective autophagy adaptor for MYH9/myosin IIA. Nature communications 48 34078910
2003 Nek9, a novel FACT-associated protein, modulates interphase progression. The Journal of biological chemistry 44 14660563
2023 Cancer associated fibroblast derived SLIT2 drives gastric cancer cell metastasis by activating NEK9. Cell death & disease 41 37443302
2021 The short isoform of PRLR suppresses the pentose phosphate pathway and nucleotide synthesis through the NEK9-Hippo axis in pancreatic cancer. Theranostics 41 33664869
2016 Recessive NEK9 mutation causes a lethal skeletal dysplasia with evidence of cell cycle and ciliary defects. Human molecular genetics 40 26908619
2016 Somatic Mutations in NEK9 Cause Nevus Comedonicus. American journal of human genetics 40 27153399
2017 Dabrafenib inhibits the growth of BRAF-WT cancers through CDK16 and NEK9 inhibition. Molecular oncology 39 29112787
2020 EML4-ALK V3 oncogenic fusion proteins promote microtubule stabilization and accelerated migration through NEK9 and NEK7. Journal of cell science 38 32184261
2021 NEK9, a novel effector of IL-6/STAT3, regulates metastasis of gastric cancer by targeting ARHGEF2 phosphorylation. Theranostics 37 33500736
2014 A gemcitabine sensitivity screen identifies a role for NEK9 in the replication stress response. Nucleic acids research 37 25217585
2013 NEK9 depletion induces catastrophic mitosis by impairment of mitotic checkpoint control and spindle dynamics. Biochemical and biophysical research communications 27 23665325
2012 Nek9 regulates spindle organization and cell cycle progression during mouse oocyte meiosis and its location in early embryo mitosis. Cell cycle (Georgetown, Tex.) 26 23159858
2013 Structural analysis of the regulation of the DYNLL/LC8 binding to Nek9 by phosphorylation. The Journal of biological chemistry 24 23482567
2011 DYNLL/LC8 protein controls signal transduction through the Nek9/Nek6 signaling module by regulating Nek6 binding to Nek9. The Journal of biological chemistry 23 21454704
2007 Adenovirus E1A proteins direct subcellular redistribution of Nek9, a NimA-related kinase. Journal of cellular physiology 21 17443675
2015 The Dual Nature of Nek9 in Adenovirus Replication. Journal of virology 19 26676776
2014 NEK9-dependent proliferation of cancer cells lacking functional p53. Scientific reports 17 25131192
2023 Overexpression of the NEK9-EG5 axis is a novel metastatic marker in pathologic stage T3 colon cancer. Scientific reports 12 36611072
2024 USP19 potentiates autophagic cell death via inhibiting mTOR pathway through deubiquitinating NEK9 in pancreatic cancer. Cell death and differentiation 10 39627360
2024 Involvement of NEK2 and NEK9 in LPS - induced endothelial barrier dysfunction. Microvascular research 9 38176677
2022 Study on the Expression of lncRNA ATB and Nek9 in Breast Cancer Patients Based on Q-PCR Technology and Its Relationship with the Disease. Contrast media & molecular imaging 4 35935324
2025 FAM49B drives colorectal cancer progression by stabilizing c-Myc through NEK9 phosphorylation. BioFactors (Oxford, England) 2 39780509
2023 Novel variants of NEK9 associated with neonatal arthrogryposis: Two case reports and a literature review. Frontiers in genetics 2 36712877
2025 NEK9-mediated Wnt signalling repressor TLE3 rewires Docetaxel resistance in cancer cells by inducing pyroptosis. British journal of cancer 1 40926063
2021 Synthesis and structure-activity relationships of targeted protein degraders for the understudied kinase NEK9. Current research in chemical biology 1 40837099
2025 Visualizing NEK9 in action: aptamer-based fluorescent probes for real-time live-cell imaging. Nucleosides, nucleotides & nucleic acids 0 40927996
2021 Giant nevus comedonicus of the entire lower left limb protecting from the associated bullous pemphigoid: A hypothetical role of NEK9 mutation? The Journal of dermatology 0 34002421
2020 Nek9,a sensitive immunohistochemical marker for Schwannian, melanocytic and myogenic tumours. Journal of clinical pathology 0 32792414

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