Affinage

NEK7

Serine/threonine-protein kinase Nek7 · UniProt Q8TDX7

Length
302 aa
Mass
34.6 kDa
Annotated
2026-06-10
100 papers in source corpus 41 papers cited in narrative 41 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NEK7 is a NIMA-related serine/threonine kinase with two largely separable cellular roles: an enzymatic role in mitotic cell division and a non-catalytic scaffolding role in innate immune signaling (PMID:19414596, PMID:26814970). As a kinase, NEK7 is activated late in a mitotic cascade in which NEK9 binds its non-catalytic C-terminal tail and phosphorylates the NEK7 activation loop (PMID:12840024); structurally, this activation works by promoting a back-to-back NEK7 dimer that displaces an autoinhibitory Tyr97 from the active site (PMID:19941817, PMID:26522158). Active NEK7 localizes to centrosomes and spindle poles and is required for centriole duplication, centrosomal microtubule nucleation, spindle assembly, and cytokinesis, with loss causing multipolar/monopolar spindles, aneuploidy, and embryonic lethality in mice (PMID:17101132, PMID:17586473, PMID:20473324, PMID:22100915). It controls microtubule dynamics directly and through substrate phosphorylation, including EML4 at Ser144/Ser146 to reduce microtubule affinity during chromosome congression and the kinesin Eg5/KIF11 to govern dendrite morphogenesis and cortical interneuron wiring (PMID:31409757, PMID:29899413, PMID:30067978, PMID:23313050). NEK7 also phosphorylates TRF1-Ser114 to block its proteasomal degradation and protect telomere integrity after oxidative damage (PMID:28216227). Independently of its kinase activity, NEK7 is an essential co-activator of the NLRP3 inflammasome, acting downstream of K+ efflux: it binds the LRR and NACHT domains of NLRP3 through its C-terminal lobe and bridges adjacent NLRP3 subunits to license oligomerization, ASC speck formation, and caspase-1 activation (PMID:26814970, PMID:26642356, PMID:31189953). A single residue, Arg121, distinguishes NEK7 from NEK6 and is required for NLRP3 binding (PMID:35354613), and small molecules engaging this interface (e.g., berberine at Arg121) selectively block NLRP3 activation (PMID:33440945, PMID:34882936). This interaction is gated by post-translational modifications on both partners—JNK1 phosphorylation of NEK7 Thr190/191 and GSTO1-1 deglutathionylation of Cys253 promote it, PLK4 phosphorylation of Ser204 suppresses it, and MARCH5-mediated K27 ubiquitination and ZDHHC5 palmitoylation of NLRP3 are required for NEK7 recruitment (PMID:39752537, PMID:31577945, PMID:31762063, PMID:37575012, PMID:38092000).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 2003 High

    Established that NEK7 sits downstream in a kinase cascade, answering how this kinase becomes activated.

    Evidence In vitro kinase reconstitution with activated NEK9 and mass spectrometry of the activation-loop site

    PMID:12840024

    Open questions at the time
    • Did not define the structural basis of activation
    • Physiological triggers of NEK9 activity not addressed
  2. 2009 High

    Defined the autoinhibition mechanism, showing how NEK7 is held inactive and how NEK9 relieves it.

    Evidence X-ray crystallography of NEK7 with Tyr97Phe mutagenesis and in vitro kinase assays

    PMID:19941817

    Open questions at the time
    • Did not capture the active conformation
    • Mechanism coupling NEK9 binding to Tyr97 release unresolved at this stage
  3. 2009 High

    Placed NEK7 in mitotic spindle formation and distinguished it functionally from the related NEK6.

    Evidence siRNA depletion, hypomorphic mutants, and immunofluorescence localization across mitosis

    PMID:19414596

    Open questions at the time
    • Spindle-pole substrates not identified
    • Basis of NEK6/NEK7 non-redundancy unexplained
  4. 2011 High

    Extended NEK7's mitotic role to centriole duplication and centrosome maturation, requiring kinase activity.

    Evidence siRNA depletion, centrosome-targeted overexpression, kinase-dead mutants, PCM marker imaging

    PMID:20473324 PMID:22100915

    Open questions at the time
    • Centrosomal substrates not identified
    • Link between kinase activity and PCM accumulation mechanistic detail missing
  5. 2015 High

    Resolved how NEK9 activates NEK7 structurally, linking dimerization to Tyr97 displacement.

    Evidence Crystal structure of NEK7(Y97F)–NEK9 peptide complex with mutagenesis and kinase assays

    PMID:26522158

    Open questions at the time
    • In-cell relevance of the back-to-back dimer not directly visualized
    • Does not address scaffolding (kinase-independent) functions
  6. 2015 High

    Identified NEK7 as an essential, specific NLRP3 inflammasome component and revealed its mitosis–inflammasome mutual exclusivity.

    Evidence Genome-wide CRISPR screen, Co-IP with domain mapping, siRNA, cell-cycle synchronization, in vivo models

    PMID:26553871 PMID:26642356

    Open questions at the time
    • Whether kinase activity is required not fully settled at this point
    • Structural basis of the NEK7–NLRP3 interface unknown
  7. 2016 High

    Established that NEK7's scaffolding role, not its catalytic activity, mediates NLRP3 activation downstream of K+ efflux.

    Evidence Reciprocal Co-IP, Nek7-/- macrophages and chimeras, kinase-dead rescue, ASC speck imaging

    PMID:26814970

    Open questions at the time
    • Structural mechanism of subunit bridging not yet resolved
    • How K+ efflux promotes the interaction unknown
  8. 2017 High

    Revealed a telomere-protective kinase function, showing NEK7 phosphorylates TRF1 to block its degradation.

    Evidence In vitro kinase assay with Ser114 mutagenesis, Co-IP, ChIP, mass spectrometry, KO cells

    PMID:28216227

    Open questions at the time
    • Signal coupling ATM activation to NEK7 telomere recruitment incompletely defined
    • Relationship to NEK7's centrosomal pool unclear
  9. 2019 High

    Provided the structural basis for NEK7-mediated NLRP3 oligomerization via bipartite interactions.

    Evidence 3.8 Å cryo-EM of inactive NLRP3–NEK7 with interface mutagenesis and cell rescue

    PMID:31189953

    Open questions at the time
    • Structure of the active oligomer was modeled, not directly resolved
    • Conformational steps from binding to oligomerization not captured
  10. 2019 High

    Identified mitotic and neuronal substrates of NEK7 kinase activity that control microtubule behavior.

    Evidence In vitro kinase assays with phosphosite mutants for EML4 (S144/S146) and Eg5, microtubule and morphology imaging in vitro and in vivo

    PMID:23313050 PMID:29899413 PMID:30067978 PMID:31409757

    Open questions at the time
    • Full substrate repertoire not defined
    • How NEK7 selects spindle vs dendritic targets unknown
  11. 2019 High

    Began defining the PTM code on NEK7 and NLRP3 that tunes inflammasome assembly, including suppressive PLK4 phosphorylation and activating deglutathionylation.

    Evidence In vitro kinase and deglutathionylation assays, site-specific mutagenesis (S204, Cys253), Co-IP, KO mice and macrophages

    PMID:31577945 PMID:31762063

    Open questions at the time
    • Hierarchy and interplay among NEK7 PTMs unresolved
    • Spatial coordination with centrosomal PLK4 in immune cells unclear
  12. 2022 High

    Pinpointed Arg121 as the determinant of NLRP3 binding specificity that explains why NEK7, not NEK6, supports the inflammasome.

    Evidence Reciprocal single-residue swap (R121/Q132) with Co-IP and caspase-1 assays in macrophages

    PMID:35354613

    Open questions at the time
    • Does not address whether other contacts contribute under physiological conditions
  13. 2023 High

    Showed that NLRP3-side modifications—K27 ubiquitination and palmitoylation—are prerequisites for NEK7 recruitment.

    Evidence Linkage-specific ubiquitination and acyl-RAC assays, site mutagenesis (K324/K430; LRR palmitoylation), Co-IP, conditional/KO mice

    PMID:37575012 PMID:38092000

    Open questions at the time
    • Temporal ordering relative to K+ efflux not fully resolved
    • Whether these modifications act on the same NLRP3 pool simultaneously unknown
  14. 2024 Medium

    Expanded the regulatory layer with additional NEK7 PTMs and mRNA-level control governing its abundance and NLRP3 binding.

    Evidence Co-IP, K48-ubiquitination and O-GlcNAc assays, site mutagenesis (K189/K293; S260), m6A-RIP/luciferase, KO macrophages and disease models

    PMID:38389178 PMID:38696610 PMID:38865260

    Open questions at the time
    • Many sites from single labs without cross-validation
    • Quantitative contribution of each PTM in vivo unclear
  15. 2024 Medium

    Identified non-canonical NEK7 kinase functions in cancer, including EML4-ALK-associated migration and EGFR-driven drug resistance.

    Evidence CRISPR screens, Co-IP, phosphosite analysis (Eg5 S1033; EGFR S1070), inhibitory peptide, organoid/xenograft models

    PMID:32184261 PMID:38458397 PMID:40694824

    Open questions at the time
    • Single-lab findings
    • Generality across tumor types and direct kinase-substrate relationships need broader validation
  16. 2025 Medium

    Tested NEK7's necessity for NLRP3 activation pharmacologically and revealed context- and species-dependence.

    Evidence JNK1 phosphosite knock-in mice (T190V/T191V), carbamoylation MS, and a CRBN glue degrader across human cells, mice, and monkeys

    PMID:39752537 PMID:40053593 PMID:40639372

    Open questions at the time
    • Why NEK7 requirement varies across primates/conditions unresolved
    • Existence of NEK7-independent NLRP3 activation modes not fully defined

Open questions

Synthesis pass · forward-looking unresolved questions
  • How NEK7's two roles—mitotic kinase and inflammasome scaffold—are coordinated spatially and temporally within a single cell, and whether NEK7 is absolutely required for NLRP3 activation across species, remains open.
  • No unified model linking cell-cycle sequestration of NEK7 to inflammasome timing at the molecular level
  • Species-dependent dispensability suggests redundancy that is uncharacterized

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 5 GO:0060090 molecular adaptor activity 3 GO:0008092 cytoskeletal protein binding 2 GO:0140657 ATP-dependent activity 2
Localization
GO:0005815 microtubule organizing center 3 GO:0005856 cytoskeleton 2 GO:0005694 chromosome 1 GO:0005829 cytosol 1
Pathway
R-HSA-1640170 Cell Cycle 4 R-HSA-168256 Immune System 4 R-HSA-5357801 Programmed Cell Death 3 R-HSA-1852241 Organelle biogenesis and maintenance 2
Partners
A20ANKS3EGFREML4KIF11NEK9NLRP3TRF1
Complex memberships
NLRP3 inflammasome

Evidence

Reading pass · 41 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2016 NEK7 is an essential mediator of NLRP3 inflammasome activation downstream of potassium efflux. NEK7 binds directly to the catalytic domain of NLRP3, and this interaction is promoted by K+ efflux. NEK7 loss abolishes caspase-1 activation and IL-1β release in response to NLRP3 stimuli but not AIM2 or NLRC4 stimuli. Notably, the catalytic activity of NEK7 is dispensable for NLRP3 inflammasome activation; its scaffolding role is sufficient. NEK7 is required for high-molecular-mass NLRP3 complex formation and ASC oligomerization/speck formation. Co-immunoprecipitation, genetic knockout (Nek7-/- macrophages and mouse chimeras), caspase-1 activation assay, IL-1β release assay, ASC speck imaging, kinase-dead mutant rescue Nature High 26814970
2015 NEK7 binds the leucine-rich repeat (LRR) domain of NLRP3 in a kinase-independent manner downstream of mitochondrial ROS induction, and this interaction is necessary for NLRP3-ASC complex formation, ASC oligomerization, and caspase-1 activation. NLRP3 inflammasome activation is restricted to interphase because NEK7 is sequestered during mitosis, making mitosis and inflammasome activation mutually exclusive. Co-immunoprecipitation, domain mapping, siRNA knockdown, cell-cycle synchronization, in vivo peritonitis and EAE models Nature immunology High 26642356
2015 Genome-wide CRISPR/Cas9 screen in macrophages identified NEK7 as an essential and specific component of NLRP3 inflammasome activation; Nek7-deficient macrophages show blunted NLRP3 response while AIM2-mediated inflammasome activation is intact. Genome-wide CRISPR/Cas9 screen, FACS-based pyroptosis assay, targeted validation in Nek7-KO macrophages The Journal of biological chemistry High 26553871
2019 Cryo-EM structure of inactive human NLRP3 in complex with NEK7 at 3.8 Å resolution shows the C-terminal lobe of NEK7 nestles against both the LRR and NACHT domains of NLRP3. Mutagenesis of the NLRP3-NEK7 interface abolishes activation in vitro and in cells. Modeling predicts NEK7 bridges adjacent NLRP3 subunits in the active oligomer, and mutations to this secondary interface also abolish activation, suggesting NEK7 mediates NLRP3 oligomerization via bipartite interactions. Cryo-electron microscopy (3.8 Å), mutagenesis in vitro and in NEK7-KO / NLRP3-KO cell rescue assays, structural modeling based on NLRC4 inflammasome Nature High 31189953
2003 Nercc1/NEK9 directly phosphorylates NEK7 (and NEK6) on the activation loop in vitro, leading to activation of NEK7 kinase activity (~20–25-fold for NEK6; similar for NEK7). NEK7 binds the C-terminal non-catalytic tail of NEK9. This establishes a mitotic kinase cascade: NEK9 → NEK7. In vitro kinase assay, co-expression with activated NEK9 mutant, recombinant protein phosphorylation, mass spectrometry of phosphorylation site The Journal of biological chemistry High 12840024
2009 Crystal structure of NEK7 reveals an autoinhibited conformation in which Tyr97 protrudes into the active site, interacts with the activation loop, and blocks the αC helix. Mutation of Tyr97 to phenylalanine renders NEK7 constitutively active. Binding of the NEK9 non-catalytic C-terminal domain increases NEK6/NEK7 activity but not the Tyr97Phe mutant activity, indicating NEK9 activates NEK7 by releasing the autoinhibitory tyrosine. X-ray crystallography, site-directed mutagenesis, in vitro kinase assay Molecular cell High 19941817
2015 Self-association of the NEK9 C-terminal domain (CTD) is required for NEK7 activation. Crystal structure of NEK7(Y97F) bound to NEK9(810-828) reveals a binding site on the C-lobe of NEK7. NEK7(Y97F) crystallizes as a back-to-back dimer between N-lobes; this dimer interface is coupled to the conformation of residue 97. NEK9-CTD activates NEK7 by promoting back-to-back dimerization that releases the autoinhibitory Tyr97. X-ray crystallography, mutagenesis, in vitro kinase assay, binding mapping Nature communications High 26522158
2009 NEK6 and NEK7 are both activated in mitosis and are required for robust mitotic spindle formation. Depletion or expression of reduced-activity mutants causes metaphase arrest with fragile spindles; hypomorphic conditions cause cytokinesis defects. NEK6 localizes to spindle microtubules in metaphase/anaphase and to the midbody; NEK7 localizes primarily to spindle poles. Despite high similarity, NEK6 and NEK7 are non-redundant for mitotic progression. siRNA depletion, dominant-negative/hypomorphic mutant expression, immunofluorescence localization, cell cycle analysis Molecular and cellular biology High 19414596
2006 Endogenous NEK7 is enriched at the centrosome throughout the cell cycle in a microtubule-independent manner, and transiently localizes to the midbody during cytokinesis. siRNA-mediated knockdown of NEK7 causes a significant increase in cells with multipolar spindles, indicating a role in spindle assembly and mitotic progression. Immunofluorescence microscopy (endogenous protein), siRNA knockdown, microtubule depolymerization experiments FEBS letters Medium 17101132
2007 NEK7 knockdown by RNAi causes prometaphase arrest with monopolar or disorganized spindles, reduces centrosomal γ-tubulin levels, and impairs microtubule re-growth activity at the centrosome, indicating NEK7 is required for centrosomal microtubule nucleation. NEK7 also directly participates in cytokinesis, as evidenced by its midbody localization. RNAi knockdown, γ-tubulin immunofluorescence, microtubule re-growth assay, live-cell imaging Biochemical and biophysical research communications Medium 17586473
2010 Nek7-deficient mice die in late embryogenesis or early postnatal stages with severe growth retardation. MEFs from Nek7-/- embryos show chromosomal lagging, micronuclei, cytokinesis failure, tetraploidy, aneuploidy, increased multicentrosomal cells, and altered primary cilia (reduced frequency of primary cilia, appearance of two-cilia cells), confirming NEK7 as an essential regulator of cell division in vivo. Genetic knockout mouse generation, MEF cytogenetic analysis, FISH, immunofluorescence for centrosomes and cilia Oncogene High 20473324
2011 NEK7 is essential for centriole duplication: NEK7 depletion inhibits centriole duplication, and centrosome-targeted NEK7 overexpression drives extra centriole formation in a kinase-activity-dependent manner. NEK7 depletion prevents pericentriolar material (PCM) protein accumulation at centrosomes in a cell-cycle-stage-specific manner (particularly at G1/G2), indicating a role beyond mitosis in centrosome maturation. siRNA depletion, centrosome-directed overexpression constructs, kinase-dead mutant, immunofluorescence for centriole markers and PCM proteins Journal of cell science Medium 22100915
2017 NEK7 is recruited to telomeres after oxidative DNA damage in an ATM-activation-dependent manner. NEK7 phosphorylates TRF1 at Ser114, preventing TRF1 binding to Fbx4 (an SCF E3 ligase subunit) and thereby blocking TRF1 proteasomal degradation, stabilizing the TRF1-Tin2 shelterin complex at telomeres. NEK7 deficiency leads to telomere aberrations and sustained DNA damage foci after oxidative damage. Co-immunoprecipitation, in vitro kinase assay with mutagenesis (Ser114), mass spectrometry, ChIP, immunofluorescence, NEK7 KO cells Molecular cell High 28216227
2019 NEK7 regulates dendrite morphogenesis and spine formation in neurons. NEK7 kinase activity is required for dendrite growth and branching. Mechanistically, NEK7 phosphorylates the kinesin Eg5/KIF11, promoting its accumulation on microtubules in distal dendrites where Eg5 limits retrograde microtubule polymerization (inhibitory to dendrite growth), acting through microtubule stabilization independent of Eg5 motor activity. In vitro and in vivo RNAi, kinase-dead rescue, phosphomimetic/phosphodead Eg5 mutants, live-cell microtubule imaging, in vivo cortical neuron analysis Nature communications High 29899413
2019 NEK6 and NEK7 phosphorylate the N-terminal domain of the microtubule-associated protein EML4 at Ser144 and Ser146 in vitro during mitosis. This phosphorylation reduces EML4 affinity for microtubule acidic C-terminal tails. Depletion of NEK6/NEK7 increases EML4 binding to mitotic spindle microtubules, stabilizes them, and impairs chromosome congression. An S144A-S146A double mutant phenocopies kinase depletion. In vitro kinase assay, cryo-EM with 3D reconstruction (microtubule sedimentation), siRNA depletion, phosphomimetic/phosphodead mutants, chromosome congression imaging Science signaling High 31409757
2019 GSTO1-1 (glutathione transferase omega 1-1) deglutathionylates NEK7 at cysteine 253, promoting NEK7 activity and NLRP3 inflammasome activation. Inhibition or knockout of GSTO1-1 blocks NLRP3 activation, identifying glutathionylation/deglutathionylation of NEK7 Cys253 as a regulatory post-translational modification. Small molecule GSTO1-1 inhibitor (C1-27), siRNA knockdown, GSTO1-1-/- mice, site-directed mutagenesis (Cys253), biochemical deglutathionylation assay Cell reports Medium 31577945
2019 PLK4, the master regulator of centrosome duplication, phosphorylates NEK7 at Ser204. This phosphorylation attenuates NEK7-NLRP3 interaction and suppresses NLRP3 inflammasome activation. The deubiquitinase CYLD is recruited to the centrosome by Spata2 to deubiquitinate PLK4, facilitating PLK4-mediated NEK7 phosphorylation. Mutation of NEK7 Ser204 augments NEK7-NLRP3 interaction and NLRP3 activation. Co-immunoprecipitation, in vitro kinase assay, phospho-site mutagenesis (Ser204), shRNA knockdown, PLK4 pharmacological inhibition, Spata2-deficient macrophages and peritonitis model The EMBO journal High 31762063
2023 ZDHHC5 palmitoylates NLRP3 at the LRR domain; this S-palmitoylation promotes NLRP3-NEK7 interaction, NLRP3 oligomerization, and inflammasome assembly. The depalmitoylase ABHD17A reverses this modification. ZDHHC5 silencing blocks NLRP3-NEK7 interaction and ASC speck formation, and Zdhhc5-/- mice show defective NLRP3 activation in vivo. Acyl-RAC palmitoylation assay, Co-immunoprecipitation, ZDHHC5 siRNA/KO, ABHD17A functional assay, mutagenesis of palmitoylation sites, Zdhhc5-/- mice Molecular cell High 38092000
2023 MARCH5, a mitochondria-associated E3 ligase, interacts with the NACHT domain of NLRP3 and promotes K27-linked polyubiquitination of NLRP3 at K324 and K430. This ubiquitination is required for NLRP3 to bind NEK7 and form NLRP3 oligomers; ubiquitination-defective NLRP3 mutants (K324R, K430R) cannot bind NEK7 or form ASC specks. March5 conditional KO mice fail to produce IL-1β/IL-18 upon infection. Co-immunoprecipitation, ubiquitination assay with linkage-specific analysis, site-directed mutagenesis (K324R, K430R), myeloid-specific conditional KO mice, macrophage infection models The EMBO journal High 37575012
2025 NEK7 is rapidly phosphorylated at threonine-190/191 by JNK1 downstream of K+ efflux and gasdermin D (GSDMD) pore formation after NLRP3 activation. This phosphorylation enhances NEK7-NLRP3 binding and further promotes inflammasome assembly. Knock-in mice with Thr190/191Val substitutions show impaired NEK7 phosphorylation, reduced NLRP3 inflammasome activation, and decreased IL-1β secretion. Phospho-proteomics, in vitro JNK1 kinase assay, knock-in mutant mice (T190V/T191V), Co-immunoprecipitation, NLRP3 activation assays Science immunology High 39752537
2024 A20 (TNFAIP3) directly binds NEK7 and mediates K48-linked ubiquitination of NEK7 at K189 and K293, targeting NEK7 for proteasomal degradation. A20 deficiency increases NEK7 protein levels. A20 also disrupts NEK7-NLRP3 association, potentially via its OTU domain and/or ZnF4/ZnF7 motifs. NEK7 deletion attenuates NLRP3 inflammasome activation in A20-deficient conditions both in vitro and in vivo. Co-immunoprecipitation, ubiquitination assay (K48-linkage specific), site-directed mutagenesis (K189, K293), A20-deficient macrophages and in vivo models Proceedings of the National Academy of Sciences of the United States of America Medium 38865260
2024 METTL3-dependent m6A modification of NEK7 mRNA increases its stability (recognized by IGF2BP2), promoting NEK7 expression and NLRP3 inflammasome activation. Silencing METTL3 reduces A20/TNFAIP3 m6A modification, stabilizes TNFAIP3 mRNA (increasing A20 protein), which then ubiquitinates NEK7 to impair NLRP3 assembly. In a separate study, silencing METTL3 inhibits m6A methylation of NEK7, reducing NEK7 mRNA stability and blocking chondrocyte pyroptosis. m6A-RIP, RNA immunoprecipitation, dual-luciferase reporter, METTL3 KO/KD, TNFAIP3 ubiquitination assay, in vivo periodontitis model Advanced science (Weinheim, Baden-Wurttemberg, Germany) Medium 38696610
2024 OGT-mediated O-GlcNAcylation of NEK7 at S260 suppresses NEK7 phosphorylation at S260, which promotes NEK7-NLRP3 interaction and chondrocyte pyroptosis. OGT silencing enhances NEK7 phosphorylation and blocks the NEK7-NLRP3 complex, reducing NLRP3 activation. Co-immunoprecipitation, western blot for O-GlcNAc and phosphorylation, OGT knockout/knockdown, site-specific mutagenesis (S260), in vivo OA model Autoimmunity Medium 38389178
2024 SIRT5 desuccinylates NEK7 at K81, downregulating NEK7 expression/activity. Electroacupuncture in a MCAO mouse model of ischemia-reperfusion injury activates SIRT5, leading to decreased NEK7 succinylation at K81 and reduced neuronal pyroptosis. NEK7 overexpression reverses these protective effects. MCAO mouse model, succinylation assay, SIRT5 KD, NEK7 overexpression rescue, western blot, brain infarct analysis Brain research bulletin Low 39694147
2023 TRIM32 (E3 ubiquitin ligase) promotes ubiquitylation of NEK7 at K64, leading to NEK7 downregulation and suppression of NLRP3-dependent microglial pyroptosis. Inhibiting NEK7 ubiquitylation reverses TRIM32-mediated pyroptosis suppression. Co-immunoprecipitation, ubiquitylation assay, site-directed mutagenesis (K64), siRNA/overexpression, in vivo SCI mouse model Molecular biotechnology Low 38030945
2020 EML4-ALK V3 oncogenic fusion protein recruits NEK9 and NEK7 to microtubules via the EML4 N-terminal microtubule-binding region. This complex promotes microtubule stabilization, extended cytoplasmic protrusions, and increased cell migration. Constitutively active NEK9 accelerates migration in a microtubule-dependent manner requiring downstream NEK7 but not ALK activity. Co-immunoprecipitation, siRNA depletion, dominant-active NEK9 expression, cell morphology and migration assays, microtubule imaging Journal of cell science Medium 32184261
2024 In the context of EML4-ALK V3, NEK7 phosphorylates the kinesin Eg5 at Ser1033 on interphase microtubules. Eg5 phosphorylation at S1033 by NEK7 drives the mesenchymal morphology of EML4-ALK V3 cells. NEK7 depletion reduces Eg5 recruitment to microtubules, and a phosphomimetic Eg5-S1033D mutant rescues mesenchymal morphology in NEK7-depleted cells. siRNA depletion of NEK7, phosphomimetic/phosphodead Eg5 mutants, immunofluorescence for Eg5 microtubule association, cell morphology analysis The Journal of biological chemistry Medium 38458397
2017 NEK7 depletion inhibits G1 progression in human U2OS cells by downregulating cyclins and CDKs. NEK7 loss also inhibits the earliest stages of procentriole formation (STIL degradation by APC/CCdh1) and induces primary cilia formation in RPE1 cells. Abnormal APC/CCdh1 accumulation at centrioles and continuous STIL degradation are the mechanistic basis for centriole assembly failure. siRNA depletion, cell cycle analysis, immunofluorescence for centriole markers (STIL, Cdh1), quantitative microscopy Molecular biology of the cell Medium 28539406
2013 NEK7 regulates microtubule dynamic instability: siRNA-mediated NEK7 knockdown reduces speeds of MT growth and catastrophe, reduces relative time in catastrophe, and lowers overall MT dynamicity. Ectopic overexpression has inverse effects. These phenotypes are recapitulated in Nek7-/- MEFs, establishing NEK7 as a direct regulator of MT dynamics. siRNA knockdown, NEK7-/- MEFs, PlusTipTracker live-cell MT imaging, rescue by re-expression Biochimica et biophysica acta Medium 23313050
2015 Anks3 interacts with NEK7 (but not NEK6) and retains NEK7 in the cytoplasm, preventing NEK7 nuclear localization. Anks3 undergoes an ~20 kDa molecular weight increase upon NEK7 co-expression that is not caused by NEK7-dependent phosphorylation (occurs with kinase-dead NEK7 mutant), indicating an alternative modification mechanism. Co-immunoprecipitation, subcellular fractionation, immunofluorescence, kinase-dead mutant, mass spectrometry Biochemical and biophysical research communications Medium 26188091
2019 Licochalcone B (LicoB) directly binds NEK7 and inhibits the NEK7-NLRP3 interaction, specifically suppressing NLRP3 inflammasome activation without affecting AIM2 or NLRC4 inflammasomes. This is the first direct small-molecule targeting of the NEK7-NLRP3 interface as a therapeutic approach. Direct binding assay (drug-protein interaction), Co-immunoprecipitation, macrophage inflammasome activation assays, in vivo sepsis/peritonitis/NASH models EMBO reports Medium 34882936
2020 Berberine directly targets NEK7 via a hydrogen bond between its 2,3-methylenedioxy group and Arg121 of NEK7. Because R121 is located within the key domain mediating NEK7-NLRP3 interaction, berberine specifically blocks the NEK7-NLRP3 interaction and inhibits IL-1β release independently of NF-κB and TLR4 signaling. Anti-inflammatory efficacy in vivo is NEK7-dependent. Activity-based protein profiling (direct target identification), site-directed mutagenesis (R121), Co-immunoprecipitation, NEK7 KD in vivo rescue Journal of medicinal chemistry Medium 33440945
2022 A single amino acid difference—Arg121 in NEK7 versus Gln132 in NEK6—accounts for NEK7's ability to bind NLRP3 and support inflammasome activation, while NEK6 cannot. Substituting Gln132 with Arg in NEK6 confers NLRP3 binding and inflammasome activation activity in macrophages. Site-directed mutagenesis (R121/Q132), Co-immunoprecipitation, caspase-1 activation assay in macrophages, NEK7-KO rescue Journal of immunology (Baltimore, Md. : 1950) High 35354613
2019 PAF (platelet-activating factor) activates the NLRP3 inflammasome in a NEK7-dependent (and NLRP3, ASC, caspase-1-dependent) manner, requiring potassium efflux, independently of PAFR signaling. NEK7 is thus required for a lipid-triggered, PAFR-independent pathway of NLRP3 activation. Genetic KO macrophages (NEK7, NLRP3, ASC, caspase-1), K+ efflux measurement, in vivo peritonitis model The Journal of experimental medicine Medium 31558613
2019 Co-chaperone UNC45A is required for expression of NEK7 in cancer cells. UNC45A localizes to the cancer cell nucleus, upregulates glucocorticoid receptor transcriptional activity, and thereby promotes NEK7 transcription. UNC45A-deficient cancer cells show pericentrosomal material disorganization, centrosomal separation defects, and metaphase/cytokinesis stalling; these phenotypes are rescued by heterologous NEK7 expression. siRNA knockdown, immunofluorescence, gene microarray, RT-qPCR, glucocorticoid receptor activity assay, NEK7 rescue expression The Journal of biological chemistry Medium 30737284
2025 NEK7 directly binds EGFR and phosphorylates it at serine 1070, activating MAPK and PI3K/AKT signaling pathways to drive acquired lenvatinib resistance in hepatocellular carcinoma. An inhibitory peptide targeting the NEK7-binding domain (EGFR aa 979–1099) blocks EGFR S1070 phosphorylation and suppresses resistance. Kinase CRISPR-Cas9 genetic screen, Co-IP (NEK7-EGFR binding), site-specific phosphorylation analysis (S1070), inhibitory peptide design, organoid and xenograft models Hepatology (Baltimore, Md.) Medium 40694824
2025 Isocyanic acid (produced by LACC1 enzyme) carbamoylates NLRP3 at lysine-593, disrupting NLRP3-NEK7 interaction and limiting NLRP3 inflammasome activation. LACC1 KO or K593 carbamoylation-deficient NLRP3 mutant promotes inflammatory responses in vitro and in vivo. Carbamoylation mass spectrometry, site-directed mutagenesis (K593), Co-immunoprecipitation, LACC1 KO/Lacc1-/- mice, macrophage inflammasome assays Science advances Medium 40053593
2018 NEK7 is required for proper wiring of cortical parvalbumin (PV+) interneurons. NEK7-deficient PV+ interneurons show altered microtubule dynamics, impaired axon growth cone steering, reduced axon length, lower arbor complexity, and fewer synaptic contacts with pyramidal cells in vivo. Conditional/targeted NEK7 ablation in PV interneurons, in vitro and in vivo morphology analysis, microtubule dynamics live imaging, synapse quantification Cell reports Medium 30067978
2021 NLRP3 inflammasome activation by NEK7 is restricted to interphase; the NEK7-NLRP3 interaction is required for NLRP3 activation, and the interaction is enhanced by K+ efflux. The Influenza A virus accessory protein PB1-F2 limits the conformational transition of NLRP3 from its auto-repressed closed conformation to its active state, thereby diminishing NEK7-NLRP3 interaction and blocking inflammasome assembly in human macrophages. IAV mutant infection (PB1-F2 deficient), Co-immunoprecipitation (NEK7-NLRP3), caspase-1 activation assay, gasdermin D cleavage, LDH/IL-1β release EMBO reports Medium 33180976
2022 In HEK293 cells reconstituted with NLRP3 and ASC (but not NEK7), ASC speck polymerization occurs independently of NEK7, suggesting a NEK7-independent mode of NLRP3 activation exists in this cell system. Evidence supports a stacked-torus hexameric NLRP3 oligomer in this context. HEK293 cell reconstitution of NLRP3 inflammasome without NEK7, ASC speck assay, interface mutagenesis based on structural models International journal of molecular sciences Low 36142182
2025 A cereblon (CRBN) glue degrader of NEK7 (NK7-902) potently and selectively degrades NEK7 in human immune cells. Full NEK7 degradation completely blocked NLRP3-dependent IL-1β release in vitro in some but not all donors/conditions, and in mice. In cynomolgus monkeys, NK7-902 caused long-lasting NEK7 degradation but only transiently blocked IL-1β, suggesting NEK7 contributes to but may not be absolutely required for NLRP3 activation in primates. Targeted protein degradation (CRBN molecular glue), NEK7 protein level measurement in primary human cells and whole blood, NLRP3 activation assays, in vivo mouse and monkey models Cell chemical biology Medium 40639372

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2016 NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature 1081 26814970
2019 Structural mechanism for NEK7-licensed activation of NLRP3 inflammasome. Nature 653 31189953
2015 NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component. Nature immunology 632 26642356
2015 A Genome-wide CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) Screen Identifies NEK7 as an Essential Component of NLRP3 Inflammasome Activation. The Journal of biological chemistry 384 26553871
2019 NEK7 interacts with NLRP3 to modulate the pyroptosis in inflammatory bowel disease via NF-κB signaling. Cell death & disease 326 31787755
2003 A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases. The Journal of biological chemistry 154 12840024
2009 The Nek6 and Nek7 protein kinases are required for robust mitotic spindle formation and cytokinesis. Molecular and cellular biology 142 19414596
2023 ZDHHC5-mediated NLRP3 palmitoylation promotes NLRP3-NEK7 interaction and inflammasome activation. Molecular cell 122 38092000
2021 Licochalcone B specifically inhibits the NLRP3 inflammasome by disrupting NEK7-NLRP3 interaction. EMBO reports 91 34882936
2006 Nek7 kinase is enriched at the centrosome, and is required for proper spindle assembly and mitotic progression. FEBS letters 86 17101132
2020 Recent advances in the NEK7-licensed NLRP3 inflammasome activation: Mechanisms, role in diseases and related inhibitors. Journal of autoimmunity 84 32703754
2019 PLK4 deubiquitination by Spata2-CYLD suppresses NEK7-mediated NLRP3 inflammasome activation at the centrosome. The EMBO journal 81 31762063
2009 An autoinhibitory tyrosine motif in the cell-cycle-regulated Nek7 kinase is released through binding of Nek9. Molecular cell 81 19941817
2019 Glutathione Transferase Omega-1 Regulates NLRP3 Inflammasome Activation through NEK7 Deglutathionylation. Cell reports 79 31577945
2020 Berberine Directly Targets the NEK7 Protein to Block the NEK7-NLRP3 Interaction and Exert Anti-inflammatory Activity. Journal of medicinal chemistry 78 33440945
2010 Nek7 kinase targeting leads to early mortality, cytokinesis disturbance and polyploidy. Oncogene 66 20473324
2007 NEK7 is a centrosomal kinase critical for microtubule nucleation. Biochemical and biophysical research communications 60 17586473
2022 Identification of potent inhibitors of NEK7 protein using a comprehensive computational approach. Scientific reports 59 35436996
2024 Inhibition of METTL3 Alleviates NLRP3 Inflammasome Activation via Increasing Ubiquitination of NEK7. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 58 38696610
2000 Isolation and characterization of two evolutionarily conserved murine kinases (Nek6 and nek7) related to the fungal mitotic regulator, NIMA. Genomics 54 10964517
2020 NEK7: a potential therapy target for NLRP3-related diseases. Bioscience trends 53 32295992
2017 Nek7 Protects Telomeres from Oxidative DNA Damage by Phosphorylation and Stabilization of TRF1. Molecular cell 52 28216227
2020 Metformin ameliorates the NLPP3 inflammasome mediated pyroptosis by inhibiting the expression of NEK7 in diabetic periodontitis. Archives of oral biology 51 32480011
2019 Anti-inflammatory effect of artemisinin on uric acid-induced NLRP3 inflammasome activation through blocking interaction between NLRP3 and NEK7. Biochemical and biophysical research communications 50 31358323
2018 Expression and clinical significance of the NEK7-NLRP3 inflammasome signaling pathway in patients with systemic lupus erythematosus. Journal of inflammation (London, England) 50 30202244
2015 Mechanistic basis of Nek7 activation through Nek9 binding and induced dimerization. Nature communications 50 26522158
2020 NEK7 mediated assembly and activation of NLRP3 inflammasome downstream of potassium efflux in ventilator-induced lung injury. Biochemical pharmacology 46 32353421
2022 Deep Learning and Structure-Based Virtual Screening for Drug Discovery against NEK7: A Novel Target for the Treatment of Cancer. Molecules (Basel, Switzerland) 45 35807344
2019 Platelet-activating factor (PAF) mediates NLRP3-NEK7 inflammasome induction independently of PAFR. The Journal of experimental medicine 45 31558613
2020 Influenza A viruses limit NLRP3-NEK7-complex formation and pyroptosis in human macrophages. EMBO reports 44 33180976
2019 Metformin ameliorates experimental diabetic periodontitis independently of mammalian target of rapamycin (mTOR) inhibition by reducing NIMA-related kinase 7 (Nek7) expression. Journal of periodontology 42 30945296
2011 NEK7 is essential for centriole duplication and centrosomal accumulation of pericentriolar material proteins in interphase cells. Journal of cell science 42 22100915
2023 MARCH5-dependent NLRP3 ubiquitination is required for mitochondrial NLRP3-NEK7 complex formation and NLRP3 inflammasome activation. The EMBO journal 40 37575012
2020 EML4-ALK V3 oncogenic fusion proteins promote microtubule stabilization and accelerated migration through NEK9 and NEK7. Journal of cell science 38 32184261
2019 Mitotic phosphorylation by NEK6 and NEK7 reduces the microtubule affinity of EML4 to promote chromosome congression. Science signaling 38 31409757
2022 Inhibition of NEK7 Suppressed Hepatocellular Carcinoma Progression by Mediating Cancer Cell Pyroptosis. Frontiers in oncology 37 35223495
2018 NEK7 regulates dendrite morphogenesis in neurons via Eg5-dependent microtubule stabilization. Nature communications 37 29899413
2013 The expression of Nek7, FoxM1, and Plk1 in gallbladder cancer and their relationships to clinicopathologic features and survival. Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 37 23359173
2020 NEK7 Coordinates Rapid Neuroinflammation After Subarachnoid Hemorrhage in Mice. Frontiers in neurology 36 32733353
2020 Physiological and Pathological Roles of Mammalian NEK7. Frontiers in physiology 34 33364979
2016 Nek7 is overexpressed in hepatocellular carcinoma and promotes hepatocellular carcinoma cell proliferation in vitro and in vivo. Oncotarget 34 26921196
2021 Oridonin ameliorates noise-induced hearing loss by blocking NLRP3 - NEK7 mediated inflammasome activation. International immunopharmacology 32 33770730
2023 Helenine blocks NLRP3 activation by disrupting the NEK7-NLRP3 interaction and ameliorates inflammatory diseases. Phytomedicine : international journal of phytotherapy and phytopharmacology 31 37931457
2013 Nek7 kinase accelerates microtubule dynamic instability. Biochimica et biophysica acta 31 23313050
2025 NEK7 phosphorylation amplifies NLRP3 inflammasome activation downstream of potassium efflux and gasdermin D. Science immunology 30 39752537
2024 Inhibition of NLRP3 inflammasome activation by A20 through modulation of NEK7. Proceedings of the National Academy of Sciences of the United States of America 30 38865260
2019 The co-chaperone UNC45A is essential for the expression of mitotic kinase NEK7 and tumorigenesis. The Journal of biological chemistry 30 30737284
2022 NEK7: a new target for the treatment of multiple tumors and chronic inflammatory diseases. Inflammopharmacology 28 35829941
2023 ALK-JNK signaling promotes NLRP3 inflammasome activation and pyroptosis via NEK7 during Streptococcus pneumoniae infection. Molecular immunology 27 37001294
2021 NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection. Frontiers in immunology 27 34603335
2022 Rosmarinic acid alleviates acetaminophen-induced hepatotoxicity by targeting Nrf2 and NEK7-NLRP3 signaling pathway. Ecotoxicology and environmental safety 26 35753269
2003 Differential control of the NIMA-related kinases, Nek6 and Nek7, by serum stimulation. Biochemical and biophysical research communications 26 12589797
2024 Quercetin-induced pyroptosis in colon cancer through NEK7-mediated NLRP3 inflammasome-GSDMD signaling pathway activation. American journal of cancer research 25 38590424
2002 The related murine kinases, Nek6 and Nek7, display distinct patterns of expression. Mechanisms of development 25 11744387
2021 NEK7 promotes gastric cancer progression as a cell proliferation regulator. Cancer cell international 24 34419048
2015 Kinase inhibitor profile for human nek1, nek6, and nek7 and analysis of the structural basis for inhibitor specificity. Molecules (Basel, Switzerland) 24 25591119
2024 P2X7R Modulates NEK7-NLRP3 Interaction to Exacerbate Experimental Autoimmune Prostatitis via GSDMD-mediated Prostate Epithelial Cell Pyroptosis. International journal of biological sciences 21 38993566
2021 Reactive oxygen species induced by uric acid promote NRK‑52E cell apoptosis through the NEK7‑NLRP3 signaling pathway. Molecular medicine reports 21 34414459
2020 Expression of the NEK7/NLRP3 inflammasome pathway in patients with diabetic lower extremity arterial disease. BMJ open diabetes research & care 21 33323459
2017 NEK7 is required for G1 progression and procentriole formation. Molecular biology of the cell 21 28539406
2021 Betulin alleviates cisplatin-induced hepatic injury in rats: Targeting apoptosis and Nek7-independent NLRP3 inflammasome pathways. International immunopharmacology 20 34217992
2025 Anemoside B4 targets NEK7 to inhibit NLRP3 inflammasome activation and alleviate MSU-induced acute gouty arthritis by modulating the NF-κB signaling pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology 19 39939033
2023 Revisiting the inhibitory potential of protein kinase inhibitors against NEK7 protein via comprehensive computational investigations. Scientific reports 19 36922575
2022 Identification of NEK7 inhibitors: structure based virtual screening, molecular docking, density functional theory calculations and molecular dynamics simulations. Journal of biomolecular structure & dynamics 18 35983608
2024 Discovery and Development of NLRP3 Inhibitors Targeting the LRR Domain to Disrupt NLRP3-NEK7 Interaction for the Treatment of Rheumatoid Arthritis. Journal of medicinal chemistry 17 38888047
2015 Anks3 alters the sub-cellular localization of the Nek7 kinase. Biochemical and biophysical research communications 17 26188091
2024 OGT-induced O-GlcNAcylation of NEK7 protein aggravates osteoarthritis progression by enhancing NEK7/NLRP3 axis. Autoimmunity 16 38389178
2022 P2X7R-NEK7-NLRP3 Inflammasome Activation: A Novel Therapeutic Pathway of Qishen Granule in the Treatment of Acute Myocardial Ischemia. Journal of inflammation research 16 36124207
2022 miR-181a-5p Inhibits Pyroptosis in Sepsis-Induced Acute Kidney Injury through Downregulation of NEK7. Journal of immunology research 15 35991122
2021 Wolf-Hirschhorn syndrome candidate 1 facilitates alveolar macrophage pyroptosis in sepsis-induced acute lung injury through NEK7-mediated NLRP3 inflammasome activation. Innate immunity 15 34428935
2020 Nek7 conformational flexibility and inhibitor binding probed through protein engineering of the R-spine. The Biochemical journal 15 32242624
2001 Identification and assignment of the human NIMA-related protein kinase 7 gene (NEK7) to human chromosome 1q31.3. Cytogenetics and cell genetics 15 11701951
2022 Knockdown of NEK7 alleviates anterior cruciate ligament transection osteoarthritis (ACLT)-induced knee osteoarthritis in mice via inhibiting NLRP3 activation. Autoimmunity 14 35798413
2022 HDAC Inhibitor SAHA Alleviates Pyroptosis by up-regulating miR-340 to Inhibit NEK7 Signaling in Subarachnoid Hemorrhage. Neurochemical research 14 36322370
2021 An Atypical Autoinflammatory Disease Due to an LRR Domain NLRP3 Mutation Enhancing Binding to NEK7. Journal of clinical immunology 14 34671876
2018 The Microtubule Regulator NEK7 Coordinates the Wiring of Cortical Parvalbumin Interneurons. Cell reports 14 30067978
2025 A cereblon-based glue degrader of NEK7 regulates NLRP3 inflammasome in a context-dependent manner. Cell chemical biology 13 40639372
2024 NEK7 induces lactylation in Alzheimer's disease to promote pyroptosis in BV-2 cells. Molecular brain 12 39563448
2023 METTL3 Regulates the m6A Modification of NEK7 to Inhibit the Formation of Osteoarthritis. Cartilage 12 37724835
2015 Human Nek7-interactor RGS2 is required for mitotic spindle organization. Cell cycle (Georgetown, Tex.) 12 25664600
2022 MicroRNA-200c-5p targets NIMA Related Kinase 7 (NEK7) to inhibit NOD-like receptor 3 (NLRP3) inflammasome activation, MODE-K cell pyroptosis, and inflammatory bowel disease in mice. Molecular immunology 11 35447415
2022 Probing the effect of NEK7 and cofactor interactions on dynamics of NLRP3 monomer using molecular simulation. Protein science : a publication of the Protein Society 11 36173167
2024 Silencing of METTL3 inhibits m6A methylation of NEK7 to suppress pyrolysis in an HT-22 cell-based model of intracerebral hemorrhage. Brain research 10 38408556
2022 The Critical Role of Potassium Efflux and Nek7 in Pasteurella multocida-Induced NLRP3 Inflammasome Activation. Frontiers in microbiology 10 35350616
2022 The Inflammasome Activity of NLRP3 Is Independent of NEK7 in HEK293 Cells Co-Expressing ASC. International journal of molecular sciences 10 36142182
2025 Prunus mume derived extracellular vesicle-like particles alleviate experimental colitis via disrupting NEK7-NLRP3 interaction and inflammasome activation. Journal of nanobiotechnology 9 40685348
2024 RACK1 and NEK7 mediate GSDMD-dependent macrophage pyroptosis upon Streptococcus suis infection. Veterinary research 8 39334337
2024 Tilianin suppresses NLRP3 inflammasome activation in myocardial ischemia/reperfusion injury via inhibition of TLR4/NF-κB and NEK7/NLRP3. Frontiers in pharmacology 8 39508038
2023 LncRNA SNHG3 Promotes Sevoflurane-Induced Neuronal Injury by Activating NLRP3 via NEK7. Neurochemical research 8 37093343
2022 PKA and Epac1 Reduce Nek7 to Block the NLRP3 Inflammasome Proteins in the Retinal Vasculature. Investigative ophthalmology & visual science 8 35006270
2022 A Single Amino Acid Residue Defines the Difference in NLRP3 Inflammasome Activation between NEK7 and NEK6. Journal of immunology (Baltimore, Md. : 1950) 8 35354613
2023 NEK7 inhibition attenuates Aβ42-induced cognitive impairment by regulating TLR4/NF-κB and the NLRP3 inflammasome in mice. Journal of clinical biochemistry and nutrition 7 37700846
2024 Electroacupuncture inhibits neuronal pyroptosis in ischemic brain injury through modulating SIRT5-mediated NEK7 succinylation. Brain research bulletin 6 39694147
2023 TRIM32 Inhibits NEK7 Ubiquitylation-Dependent Microglia Pyroptosis After Spinal Cord Injury. Molecular biotechnology 6 38030945
2025 Targeted inhibition of NEK7 preventing sepsis-induced cardiomyopathy by inhibiting NLRP3 inflammasome. International immunopharmacology 5 40015203
2024 In silico study to identify novel NEK7 inhibitors from natural sources by a combination strategy. Molecular diversity 5 38598164
2025 Isocyanic acid-mediated NLRP3 carbamoylation reduces NLRP3-NEK7 interaction and limits inflammasome activation. Science advances 4 40053593
2025 NEK7-induced phosphorylation of EGFR on serine 1070 drives the acquired lenvatinib resistance in hepatocellular carcinoma. Hepatology (Baltimore, Md.) 4 40694824
2024 The mesenchymal morphology of cells expressing the EML4-ALK V3 oncogene is dependent on phosphorylation of Eg5 by NEK7. The Journal of biological chemistry 4 38458397
2019 Retracted Article: CircRNA PVT1 modulates cell metastasis via the miR-181a-5p/NEK7 axis and cisplatin chemoresistance through miR-181a-5p-mediated autophagy in non-small cell lung cancer. RSC advances 4 35542851

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