Affinage

KIF11

Kinesin-like protein KIF11 · UniProt P52732

Length
1056 aa
Mass
119.2 kDa
Annotated
2026-04-28
100 papers in source corpus 31 papers cited in narrative 30 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

KIF11 (Eg5) is a plus-end-directed kinesin-5 motor essential for centrosome separation, bipolar mitotic spindle assembly, and spindle elongation during cell division. Its spindle recruitment is controlled by CDK1 phosphorylation at Thr926/927 and Nek6/7 phosphorylation at Ser1033 downstream of a Plk1–Nek9 cascade, while PP2A/B55α dephosphorylates Thr926 to promote mitotic exit; additional post-translational regulation includes HDAC1-mediated deacetylation that modulates ATPase activity and RNF20/40-dependent monoubiquitination that stabilizes the protein (PMID:7753799, PMID:21642957, PMID:21522128, PMID:28487562, PMID:28392145, PMID:27557628). KIF11 sliding forces are functionally balanced by the minus-end motor HSET, opposed by Tiam1–Rac1 signaling, dampened by HMMR-mediated TPX2 inhibitory complexes, and supplemented by a parallel Kif15–TPX2 sliding module and a PRC1–KIF4A module during anaphase (PMID:10525540, PMID:20346677, PMID:29386294, PMID:19818618, PMID:33910056). Beyond mitosis, KIF11 transports β-actin mRNA in a ZBP1-dependent manner to regulate cell polarity and migration, promotes dendrite morphogenesis via NEK7 phosphorylation-dependent microtubule regulation, and acts as an inhibitory constraint on excitatory synaptic transmission in hippocampal neurons (PMID:25588836, PMID:29899413, PMID:30479371).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 1994 High

    Establishing that Eg5 is a bona fide microtubule-associated motor present on spindles and interphase microtubules resolved the question of whether kinesin-5 family members physically engage the mitotic apparatus.

    Evidence Immunofluorescence and microtubule co-pelleting from Xenopus egg extracts across developmental stages

    PMID:8026619

    Open questions at the time
    • Motor directionality and enzymatic mechanism not yet characterized
    • No functional loss-of-function data
  2. 1995 High

    Demonstrating that p34cdc2 (CDK1) phosphorylation of Thr937 is required for Eg5 spindle localization established the first regulatory switch controlling motor targeting to the mitotic apparatus.

    Evidence Site-directed mutagenesis of T937A/T937S in Xenopus A6 cells with immunofluorescence localization

    PMID:7753799

    Open questions at the time
    • Whether additional kinases contribute to centrosome-specific targeting
    • Phosphatase responsible for dephosphorylation unknown
  3. 1999 High

    Showing that simultaneous inhibition of HSET and Eg5 restores bipolarity established the balance-of-forces model, answering how opposing motors maintain spindle architecture.

    Evidence Antibody microinjection epistasis in murine oocytes and cultured cells combined with in vitro aster assembly

    PMID:10525540

    Open questions at the time
    • Whether dynein contributes as a direct Eg5 antagonist
    • Quantitative force contributions of each motor unknown
  4. 2004 High

    Kinetic dissection of the Eg5 ATPase cycle revealed that ATP-dependent microtubule dissociation is rate-limiting, distinguishing Eg5 from conventional kinesin and explaining its slow processivity.

    Evidence Transient-state fluorescent nucleotide kinetics with purified monomeric Eg5 constructs

    PMID:15247293

    Open questions at the time
    • How tetramerization alters the kinetic cycle
    • No structural explanation for the rate-limiting step
  5. 2007 High

    Homozygous Eg5 knockout causing pre-implantation lethality in mice definitively proved that Eg5 is essential for early mammalian cell division and cannot be compensated by other motors at this stage.

    Evidence Gene targeting (knockout mouse) with embryo recovery at E2.5–3.5

    PMID:17449012 PMID:18474226

    Open questions at the time
    • Cell-type-specific requirements in later development unexplored
    • Whether Kif15 can partially rescue in specific lineages
  6. 2008 High

    Identification of the TPX2 C-terminal domain as an Eg5-interacting element required for spindle pole separation, and of Parkin-mediated transcriptional repression of Eg5 via Hsp70 ubiquitination, defined two new layers of Eg5 regulation — one physical, one transcriptional.

    Evidence TPX2: co-IP and domain deletion with blastomere rescue in Xenopus; Parkin: ChIP, promoter-reporter, ubiquitination assays

    PMID:18372177 PMID:18845538

    Open questions at the time
    • Whether TPX2 inhibits Eg5 enzymatic activity directly (resolved later)
    • Physiological contexts where Parkin regulation of Eg5 is relevant in vivo
  7. 2009 High

    Discovery that Kif15 can fully substitute for Eg5 in centrosome separation when overexpressed revealed a redundant TPX2-dependent sliding pathway, explaining how some cancer cells escape Eg5 inhibitors.

    Evidence siRNA depletion and ectopic overexpression with live-cell imaging and co-IP in human cells

    PMID:19818618

    Open questions at the time
    • How Kif15 is regulated to become Eg5-independent
    • Whether endogenous Kif15 levels ever suffice without overexpression
  8. 2011 High

    Mapping dual phosphorylation inputs — CDK1 at Thr926 for spindle recruitment and Plk1→Nek9→Nek6/7 at Ser1033 for centrosome targeting — resolved how two independent kinase cascades converge to control Eg5 localization at distinct mitotic stages.

    Evidence In vitro kinase assays, phospho-specific antibodies, and siRNA/inhibitor epistasis in human cell lines

    PMID:21522128 PMID:21642957

    Open questions at the time
    • Whether other phosphorylation sites contribute
    • How actin-dependent forces oppose Plk1-driven slow separation
  9. 2015 High

    Demonstrating that KIF11 physically binds ZBP1 and transports β-actin mRNA to cell protrusions established a non-mitotic, mRNA-transport function for this motor, linking it to cell polarity and migration.

    Evidence Reciprocal co-IP, RNA immunoprecipitation, smFISH colocalization, shRNA knockdown with rescue, migration assays

    PMID:25588836

    Open questions at the time
    • Whether other mRNAs are KIF11 cargo
    • Structural basis for KIF11–ZBP1 interaction
  10. 2015 High

    Single-molecule reconstitution showed TPX2 directly inhibits Eg5 velocity through both motor interaction and microtubule binding, resolving the mechanism by which TPX2 dampens Eg5-dependent forces in the spindle.

    Evidence TIRF single-molecule motility and microtubule gliding assays with full-length and truncated TPX2

    PMID:26018074

    Open questions at the time
    • Quantitative contribution of TPX2 inhibition to spindle force balance in vivo
    • Which TPX2 residues mediate the interaction
  11. 2016 High

    Identification of RNF20/40-mediated monoubiquitination as a stabilizing modification for Eg5 during mitosis, and Aurora A–Kif15 as a parallel spindle pathway, expanded the regulatory landscape beyond phosphorylation and defined genetic determinants of Eg5-independent spindle assembly.

    Evidence In vitro ubiquitination assays, co-IP, and genome-wide siRNA screen in Eg5-independent cells with Aurora A kinase validation

    PMID:27354041 PMID:27557628

    Open questions at the time
    • Ubiquitination site(s) on Eg5 not mapped
    • How cells switch between Eg5-dependent and Kif15-dependent modes
  12. 2017 High

    Discovery that HDAC1 deacetylates Eg5 to regulate its ATPase activity, and that PP2A/B55α dephosphorylates Thr926 to enable mitotic exit, defined the enzymatic erasers that reset Eg5 post-translational modifications after mitotic entry.

    Evidence HDAC1 substrate trapping, ATPase assays, phosphatase assays, phosphomimetic mutant live imaging

    PMID:28392145 PMID:28487562

    Open questions at the time
    • Specific acetylation site(s) on Eg5 not identified
    • Whether other phosphatases contribute at different mitotic stages
  13. 2018 High

    Multiple studies extended KIF11 function to postmitotic neurons: NEK7 phosphorylation promotes Eg5-dependent dendrite morphogenesis by restraining retrograde microtubule polymerization, and KIF11 knockdown increases excitatory synaptic transmission, revealing a synaptic inhibitory role.

    Evidence In vitro kinase assays, neuronal RNAi/KO in vivo, electrophysiology in hippocampal neurons

    PMID:29899413 PMID:30479371

    Open questions at the time
    • Mechanism linking KIF11 to synaptic vesicle release or receptor density
    • Whether motor activity or microtubule-organizing function mediates the synaptic phenotype
  14. 2021 High

    Demonstration that Eg5 and PRC1-dependent KIF4A constitute two mechanistically distinct antiparallel microtubule sliding modules during anaphase B resolved how chromosome segregation proceeds through redundant force generators.

    Evidence Combined siRNA/pharmacological/CRISPR disruption with tubulin photoactivation and STED/expansion microscopy

    PMID:33910056

    Open questions at the time
    • How the two modules are temporally coordinated
    • Whether additional sliding motors contribute
  15. 2023 High

    Finding that cytoplasmic NEAT1 lncRNA binds KIF11 and promotes its degradation to induce senescence via CDKN2A upregulation identified a non-coding RNA-based mechanism for KIF11 turnover outside of mitosis.

    Evidence RNA pulldown, mass spectrometry, co-IP, RNA FISH, ChIP, mouse xenograft model

    PMID:37752791

    Open questions at the time
    • Degradation pathway (proteasomal vs lysosomal) not defined
    • Whether NEAT1-mediated KIF11 degradation occurs in non-tumor cell senescence

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structural basis for KIF11 tetramer-mediated antiparallel microtubule sliding at atomic resolution, the identity of specific acetylation sites modulated by HDAC1, the full cargo repertoire of KIF11 in mRNA transport, and how motor versus non-motor activities of KIF11 are partitioned in postmitotic neurons.
  • No high-resolution cryo-EM structure of full-length tetrameric KIF11 on microtubules
  • Acetylation site mapping incomplete
  • Complete mRNA cargo inventory lacking

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003774 cytoskeletal motor activity 2 GO:0008092 cytoskeletal protein binding 2 GO:0003723 RNA binding 1 GO:0140657 ATP-dependent activity 1
Localization
GO:0005815 microtubule organizing center 4 GO:0005856 cytoskeleton 3 GO:0005829 cytosol 2
Pathway
R-HSA-1640170 Cell Cycle 6 R-HSA-112316 Neuronal System 2 R-HSA-392499 Metabolism of proteins 2
Partners
HDAC1HMMRKIF15NEK7PTENRNF20TPX2ZBP1
Complex memberships
Eg5 homotetramerTPX2–Eg5 inhibitory complexZBP1–β-actin mRNP

Evidence

Reading pass · 30 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1995 Eg5 (KIF11) spindle localization is cell-cycle-regulated and requires phosphorylation at a conserved threonine (T937) in the C-terminal domain; mutation of T937 to non-phosphorylatable alanine abolishes spindle localization, while T937S preserves it, implicating p34cdc2 phosphorylation as the regulatory mechanism. Transient transfection of myc-tagged Eg5 deletion/point mutants in Xenopus A6 cells; immunofluorescence localization Proceedings of the National Academy of Sciences of the United States of America High 7753799
1994 Eg5 protein associates with both spindle and interphase microtubules during Xenopus early development, concentrating toward spindle poles during meiosis and mitosis; copellets with microtubules from egg homogenates, demonstrating direct microtubule association. Immunofluorescence and microtubule co-pelleting from Xenopus egg extracts; Western blotting across developmental stages Developmental biology High 8026619
1998 Eg5 (KIF11) continues to be expressed in postmitotic rodent neurons, where it associates directly with the microtubule array and is enriched in distal regions of developing axons and dendrites, suggesting a role in regulating microtubule organization in neuronal processes. Immunofluorescence and subcellular fractionation in rodent neurons; expression profiling across developmental stages The Journal of neuroscience Medium 9742151
1999 HSET (minus-end motor) and Eg5 (KIF11, plus-end motor) act antagonistically in spindle assembly: simultaneous inhibition of both motors in cells restores centrosome separation and bipolar spindle formation that is blocked by Eg5 inhibition alone, establishing a balance-of-forces model for spindle bipolarity. Antibody microinjection in murine oocytes and cultured cells; immunoEM localization; in vitro aster assembly assays The Journal of cell biology High 10525540
2004 Monomeric human Eg5 is a slow plus-end-directed ATPase motor; kinetic analysis shows mantATP binds at 2–3 µM⁻¹s⁻¹, ATP hydrolysis occurs at 15 s⁻¹, and ATP-dependent microtubule dissociation is rate-limiting at ~8 s⁻¹, with mantADP release at 40 s⁻¹, distinguishing Eg5 mechanism from minus-end motors where ADP release is rate-limiting. In vitro ATPase kinetics (steady-state and transient), analytical ultracentrifugation, gel filtration, fluorescent nucleotide (mantATP/mantADP) binding assays with purified Eg5-367 and Eg5-437 constructs The Journal of biological chemistry High 15247293
2008 TPX2 interacts with Eg5 via its C-terminal domain; this interaction is essential for Eg5-dependent spindle pole segregation, as TPX2-CT injection arrests spindle poles and Eg5 co-injection rescues cleavage, defining a discrete Eg5-interacting domain in TPX2. Blastomere microinjection in Xenopus embryos; cell transfection; co-immunoprecipitation; deletion analysis of TPX2 Current biology : CB High 18372177
2008 Parkin (E3 ubiquitin ligase) represses Eg5 transcription by causing multiple monoubiquitination of Hsp70, which inactivates JNK, reducing c-Jun phosphorylation and thereby blocking c-Jun binding to the AP-1 site in the Eg5 promoter; this is not via proteasomal Eg5 degradation. Co-immunoprecipitation, ubiquitination assays, Eg5 promoter-reporter assay, ChIP, siRNA knockdown, Western blotting The Journal of biological chemistry High 18845538
2009 Kif15 (kinesin-12) cooperates with Eg5 to promote bipolar spindle assembly; Kif15 can fully replace Eg5 for centrosome separation when overexpressed, and this activity requires both its motor domain and interaction with the microtubule-associated protein TPX2, suggesting a Kif15–TPX2 complex slides antiparallel microtubules apart. siRNA depletion, ectopic overexpression, live-cell imaging, co-immunoprecipitation in human cell lines Current biology : CB High 19818618
2010 Tiam1–Rac1 signaling at centrosomes during prophase functions as the first identified antagonist of Eg5-driven centrosome separation; Tiam1/Rac1 depletion allows cells to escape Eg5-inhibitor-induced mitotic arrest, and re-inhibition of Eg5 in Tiam1-depleted cells rescues chromosome congression errors, placing Tiam1–Rac upstream of Eg5 forces in bipolar spindle assembly. siRNA depletion in culture cells, live-cell imaging, Rac1-deficient epithelial cells in vivo, epistasis double-inhibition experiments Current biology : CB High 20346677
2011 CDK1 phosphorylates Eg5 at Thr926 (T927), and Plk1 controls centrosome separation additionally through activation of the Nek9–Nek6/7 kinase cascade, which phosphorylates Eg5 at Ser1033; both CDK1 (Thr926) and Nek6/7 (Ser1033) phosphorylation sites contribute to Eg5 accumulation at centrosomes and are necessary for centrosome separation. In vitro kinase assays, phospho-specific antibodies, siRNA/inhibitor epistasis, immunofluorescence in human cell lines The EMBO journal High 21642957
2011 Plk1 controls Eg5 localization to the centrosome in G2 phase and triggers centrosome separation independently of CDK1; CDK1 promotes fast centrosome separation while Plk1-driven separation is slow and opposed by actin-dependent forces; Cdk2 can also phosphorylate Eg5 at Thr927. Plk1/CDK1/CDK2 inhibitors, RNAi, phospho-specific antibodies, live-cell imaging, pharmacological manipulation of microtubule and actin dynamics The EMBO journal High 21522128
2012 Dynein does not directly antagonize Eg5 via a simple push-pull mechanism at the spindle equator; instead, live-cell imaging shows dynein and Eg5 activities cannot be titrated against each other, indicating indirect antagonism at different spindle locations. Live-cell imaging, endpoint quantification controls, mathematical modeling, siRNA depletion of dynein/Lis1 Cell reports Medium 22832270
2014 Kinetochore-microtubule stability governs the requirement for Eg5 in metaphase bipolar spindle maintenance; cells with more stable K-fibers can maintain bipolar spindles without Eg5, while artificial destabilization of K-MTs promotes spindle collapse without Eg5, and stabilization rescues bipolarity. Sibling cell line comparison, pharmacological K-MT stabilization/destabilization, Eg5 inhibitor treatment, immunofluorescence quantification Molecular biology of the cell Medium 24807901
2015 KIF11 physically interacts with ZBP1 (IGF2BP1) and is a component of β-actin mRNP complexes; KIF11 colocalizes with β-actin mRNA and its ability to transport β-actin mRNA is dependent on ZBP1; disruption of the KIF11–ZBP1 interaction in vivo delocalizes β-actin mRNA and impairs cell migration. Co-immunoprecipitation, RNA immunoprecipitation, in vitro binding assay with domain mapping, smFISH colocalization, shRNA knockdown with rescue, migration assay Journal of cell science High 25588836
2015 TPX2 inhibits Eg5 motor activity through two mechanisms: direct interaction with Eg5 (requiring dimerization or the neck region) and binding to microtubules; full-length TPX2 dramatically reduces Eg5 velocity at single-molecule level, while truncated TPX2 lacking the Eg5-interaction domain is less inhibitory. TIRF single-molecule motility assays with mammalian cell extracts expressing Eg5-EGFP; microtubule gliding assays; truncation analysis of TPX2 The Journal of biological chemistry High 26018074
2016 The RNF20/40 ubiquitin ligase complex interacts with Eg5 during mitosis and monoubiquitinates Eg5, stabilizing it; loss of RNF20/40 causes spindle assembly defects, cell cycle arrest and apoptosis, and depletes Eg5 protein levels. Co-immunoprecipitation, in vitro ubiquitination assay, siRNA knockdown, Western blotting, spindle phenotype analysis Nature communications High 27557628
2016 PTEN associates and co-localizes with EG5 during mitosis; PTEN deficiency causes aberrant EG5 phosphorylation and abrogates EG5 recruitment to the mitotic spindle, leading to shorter spindles and chromosome misalignment. Co-immunoprecipitation, immunofluorescence co-localization, PTEN siRNA knockdown, phospho-EG5 Western blotting Nature communications High 27492783
2017 HDAC1 deacetylates Eg5 (KIF11); HDAC1 co-localizes with Eg5 during mitosis and influences its ATPase activity; HDAC1/2-selective inhibition causes mitotic arrest and monopolar spindle formation consistent with Eg5 acetylation impairing function, identifying Eg5 as a non-histone HDAC1 substrate regulating mitotic progression. HDAC1 trapping mutant strategy to identify substrates, co-localization by immunofluorescence, ATPase activity assay, HDAC inhibitor treatment with spindle phenotype analysis Cell chemical biology High 28392145
2017 PP2A/B55α complex physically associates with the EG5 C-terminal tail domain and dephosphorylates EG5 at Thr926, enabling mitotic exit; PP2A knockdown leads to elevated phospho-EG5 in late metaphase and delayed mitotic exit, similar to EG5/T926D phosphomimetic mutant. Co-immunoprecipitation, in vitro phosphatase assay, phospho-specific antibodies, siRNA knockdown, EG5 phosphomimetic mutant transfection, live-cell imaging Scientific reports High 28487562
2018 NEK7 phosphorylates Eg5/KIF11, promoting its accumulation on microtubules in distal dendrites; in this postmitotic context, Eg5 limits retrograde microtubule polymerization independently of its motor activity, thereby promoting dendrite growth and branching. In vitro kinase assay, RNAi knockdown in neurons (in vitro and in vivo), NEK7 kinase-dead mutant, pharmacological Eg5 inhibition, live imaging of microtubule dynamics Nature communications High 29899413
2018 HMMR dampens Eg5-mediated forces by localizing TPX2 and promoting formation of inhibitory TPX2–Eg5 complexes; HMMR silencing disturbs spindle microtubule organization and causes elevated aneuploidy, and Eg5 chemical inhibition rescues spindle defects in HMMR-depleted cells. siRNA silencing, co-immunoprecipitation, Eg5 inhibitor rescue, immunofluorescence, CRISPR deletion Molecular biology of the cell High 29386294
2014 HIV-1 Tat protein interacts with Eg5 and allosterically modulates its ATPase activity by affecting ADP release; lysine 85 in the Tat C-terminus is critical for this interaction and for Tat-induced mitotic arrest and apoptosis in CD4+ T lymphocytes. Co-immunoprecipitation, ATPase activity assay (ADP release kinetics), site-directed mutagenesis of Tat K85, siRNA, cell cycle/apoptosis analysis The Journal of pathology High 24488929
2021 EG5/kinesin-5 together with PRC1-dependent KIF4A/kinesin-4 constitute two mechanistically distinct microtubule-sliding modules that drive spindle elongation in human cells; disruption of both EG5 and KIF4A blocks midzone microtubule sliding and causes total failure of chromosome segregation despite poleward chromosome motion. Combined siRNA depletion and pharmacological inactivation, CRISPR, tubulin photoactivation, STED microscopy, expansion microscopy Developmental cell High 33910056
2018 KIF11 knockdown in hippocampal neurons increases excitatory post-synaptic current frequency and amplitude, enhances dendritic arborization and synapse number, and requires pre-synaptic NMDAR activity for its effect; expression of Piccolo (active zone protein) constrains KIF11 function in synaptic transmission. RNAi-mediated knockdown, electrophysiological recordings (EPSCs) in hippocampal neurons, immunofluorescence morphometry, NMDAR pharmacological blockade Scientific reports Medium 30479371
2007 Homozygous knockout of Eg5 (Knsl1) causes early embryonic lethality prior to implantation in mice, with reduced cell numbers and failure of compaction; heterozygous mice are normal and fertile, establishing that Eg5 is essential for early mammalian cell division. Gene targeting (knockout mouse), embryo recovery at E2.5–3.5, morphological analysis Biochemical and biophysical research communications High 17449012 18474226
2016 Aurora A promotes bipolar spindle assembly in Eg5-independent cells by phosphorylating Kif15, enhancing Kif15 localization to the spindle; MCAK and Kif18b promote bipolar spindle assembly by destabilizing astral microtubules, placing Aurora A and Kif15 in a parallel pathway to Eg5 for centrosome separation. Genome-wide siRNA screen in Eg5-independent cells, Aurora A kinase assays, live-cell imaging, phospho-Kif15 antibodies Chromosoma High 27354041
2020 Postnatal endothelial cell-specific conditional knockout of Kif11 in mice leads to severely stunted retinal vasculature growth and mildly stunted cerebellar vasculature, demonstrating that Kif11 function in mitotically active endothelial cells is most critical in rapidly growing CNS regions and that retinal hypovascularization can arise from impaired mitotic spindle function independently of Wnt/β-catenin signaling. Conditional knockout mouse (Cre-lox), retinal vascular whole-mount imaging, β-catenin signaling analysis Human molecular genetics High 31993640
2023 NEAT1 lncRNA, when it translocates to the cytosol during senescence, interacts with KIF11 motor protein and promotes KIF11 protein degradation; KIF11 degradation leads to increased CDKN2A (p16/p14ARF) expression, inducing cellular senescence in hepatoma cells. RNA pulldown, mass spectrometry, co-immunoprecipitation, RNA FISH/immunofluorescence co-localization, ChIP, luciferase reporter assay, siRNA/shRNA knockdown, mouse xenograft model Clinical and translational medicine High 37752791
2020 PAK6 specifically co-localizes with Eg5 at the centrosome; PAK6 depletion upregulates Eg5 and causes multipolar spindles, while simultaneous Eg5 knockdown rescues this phenotype, establishing that PAK6 and Eg5 negatively inter-regulate each other to control spindle formation. Co-localization by immunofluorescence, siRNA knockdown, genetic epistasis (double knockdown rescue), Western blotting Biochimica et biophysica acta. Molecular cell research Medium 33098954
2018 Death receptor 6 (DR6) physically interacts with KIF11, as confirmed by mass spectrometry co-purification, co-immunoprecipitation, and GST pull-down; overexpression of KIF11 eliminates suppression of ovarian cancer cell migration by DR6 knockdown, placing KIF11 downstream of DR6 in a pro-migratory signaling complex. Mass spectrometry, co-immunoprecipitation, GST pull-down, overexpression rescue assay, migration assay FEBS open bio Medium 30186750

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1999 The kinesin-related protein, HSET, opposes the activity of Eg5 and cross-links microtubules in the mammalian mitotic spindle. The Journal of cell biology 274 10525540
1995 Mutations in the kinesin-like protein Eg5 disrupting localization to the mitotic spindle. Proceedings of the National Academy of Sciences of the United States of America 243 7753799
2009 Kif15 cooperates with eg5 to promote bipolar spindle assembly. Current biology : CB 226 19818618
2011 Nek9 is a Plk1-activated kinase that controls early centrosome separation through Nek6/7 and Eg5. The EMBO journal 147 21642957
2015 The mitotic kinesin KIF11 is a driver of invasion, proliferation, and self-renewal in glioblastoma. Science translational medicine 134 26355032
2014 Phenotypic overlap between familial exudative vitreoretinopathy and microcephaly, lymphedema, and chorioretinal dysplasia caused by KIF11 mutations. JAMA ophthalmology 112 25124931
2013 Advances in the discovery of kinesin spindle protein (Eg5) inhibitors as antitumor agents. European journal of medicinal chemistry 92 23434636
2020 Eg5 targeting agents: From new anti-mitotic based inhibitor discovery to cancer therapy and resistance. Biochemical pharmacology 87 33310050
2011 Differential control of Eg5-dependent centrosome separation by Plk1 and Cdk1. The EMBO journal 86 21522128
2009 K858, a novel inhibitor of mitotic kinesin Eg5 and antitumor agent, induces cell death in cancer cells. Cancer research 82 19351824
2010 Ectopic expression of the microtubule-dependent motor protein Eg5 promotes pancreatic tumourigenesis. The Journal of pathology 77 20455257
2004 Mechanistic analysis of the mitotic kinesin Eg5. The Journal of biological chemistry 77 15247293
1998 Expression of the mitotic motor protein Eg5 in postmitotic neurons: implications for neuronal development. The Journal of neuroscience : the official journal of the Society for Neuroscience 76 9742151
2013 Microcephaly with or without chorioretinopathy, lymphoedema, or mental retardation (MCLMR): review of phenotype associated with KIF11 mutations. European journal of human genetics : EJHG 71 24281367
2015 Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility. Journal of cell science 67 25588836
2008 Spindle pole regulation by a discrete Eg5-interacting domain in TPX2. Current biology : CB 63 18372177
2016 Ubiquitin ligase RNF20/40 facilitates spindle assembly and promotes breast carcinogenesis through stabilizing motor protein Eg5. Nature communications 58 27557628
2018 KIF11 Functions as an Oncogene and Is Associated with Poor Outcomes from Breast Cancer. Cancer research and treatment 57 30590004
2021 Microtubule-sliding modules based on kinesins EG5 and PRC1-dependent KIF4A drive human spindle elongation. Developmental cell 54 33910056
2013 Impaired angiogenesis and tumor development by inhibition of the mitotic kinesin Eg5. Oncotarget 54 24327603
2007 Polymorphisms in the IDE-KIF11-HHEX gene locus are reproducibly associated with type 2 diabetes in a Japanese population. The Journal of clinical endocrinology and metabolism 52 17971426
2021 KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer. International journal of biological sciences 51 33613109
2015 Dual antitumoral potency of EG5 siRNA nanoplexes armed with cytotoxic bifunctional glutamyl-methotrexate targeting ligand. Biomaterials 50 26584350
1994 The kinesin-related protein Eg5 associates with both interphase and spindle microtubules during Xenopus early development. Developmental biology 50 8026619
2010 Tiam1-Rac signaling counteracts Eg5 during bipolar spindle assembly to facilitate chromosome congression. Current biology : CB 48 20346677
2006 Eg5 expression is closely correlated with the response of advanced non-small cell lung cancer to antimitotic agents combined with platinum chemotherapy. Lung cancer (Amsterdam, Netherlands) 47 16934364
2016 Identification of novel KIF11 mutations in patients with familial exudative vitreoretinopathy and a phenotypic analysis. Scientific reports 43 27212378
2016 PTEN regulates EG5 to control spindle architecture and chromosome congression during mitosis. Nature communications 43 27492783
2014 Congenital microcephaly and chorioretinopathy due to de novo heterozygous KIF11 mutations: five novel mutations and review of the literature. American journal of medical genetics. Part A 43 25115524
2007 The kinesin related motor protein, Eg5, is essential for maintenance of pre-implantation embryogenesis. Biochemical and biophysical research communications 42 17449012
2006 Inhibition of the mitotic kinesin Eg5 up-regulates Hsp70 through the phosphatidylinositol 3-kinase/Akt pathway in multiple myeloma cells. The Journal of biological chemistry 42 16627469
2006 Regulation and targeting of Eg5, a mitotic motor protein in blast crisis CML: overcoming imatinib resistance. Cell cycle (Georgetown, Tex.) 42 16969080
2019 Identification of KIF11 As a Novel Target in Meningioma. Cancers 41 30991738
2011 Dimethylenastron suppresses human pancreatic cancer cell migration and invasion in vitro via allosteric inhibition of mitotic kinesin Eg5. Acta pharmacologica Sinica 41 21986572
2023 LncRNA NEAT1 suppresses cellular senescence in hepatocellular carcinoma via KIF11-dependent repression of CDKN2A. Clinical and translational medicine 39 37752791
2016 Overexpression of KIF11 in Gastric Cancer with Intestinal Mucin Phenotype. Pathobiology : journal of immunopathology, molecular and cellular biology 39 27459100
2008 Disruption of the mitotic kinesin Eg5 gene (Knsl1) results in early embryonic lethality. Biochemical and biophysical research communications 39 18474226
2008 Parkin regulates Eg5 expression by Hsp70 ubiquitination-dependent inactivation of c-Jun NH2-terminal kinase. The Journal of biological chemistry 38 18845538
2017 HDAC Inhibitor-Induced Mitotic Arrest Is Mediated by Eg5/KIF11 Acetylation. Cell chemical biology 37 28392145
2017 KIF11 is required for proliferation and self-renewal of docetaxel resistant triple negative breast cancer cells. Oncotarget 37 29190901
2014 Kinetochore-microtubule stability governs the metaphase requirement for Eg5. Molecular biology of the cell 37 24807901
2018 NEK7 regulates dendrite morphogenesis in neurons via Eg5-dependent microtubule stabilization. Nature communications 36 29899413
2017 KIF11 silencing and inhibition induces chromosome instability that may contribute to cancer. Genes, chromosomes & cancer 36 28510357
2008 Effects of Eg5 knockdown on human prostate cancer xenograft growth and chemosensitivity. The Prostate 36 18512732
2014 Synergism between inhibitors of Aurora A and KIF11 overcomes KIF15-dependent drug resistance. Molecular oncology 35 24950801
2013 The expression of Eg5 predicts a poor outcome for patients with renal cell carcinoma. Medical oncology (Northwood, London, England) 33 23371254
2015 TPX2 Inhibits Eg5 by Interactions with Both Motor and Microtubule. The Journal of biological chemistry 32 26018074
2016 Aurora A, MCAK, and Kif18b promote Eg5-independent spindle formation. Chromosoma 31 27354041
2020 A mouse model for kinesin family member 11 (Kif11)-associated familial exudative vitreoretinopathy. Human molecular genetics 30 31993640
2017 KIF11 Is Required for Spheroid Formation by Oesophageal and Colorectal Cancer Cells. Anticancer research 30 28011472
2016 Kinesin family members KIF11 and KIF23 as potential therapeutic targets in malignant pleural mesothelioma. International journal of oncology 30 27279560
2015 A Potent Chemotherapeutic Strategy with Eg5 Inhibitor against Gemcitabine Resistant Bladder Cancer. PloS one 28 26658059
2018 Kinesin Family of Proteins Kif11 and Kif21B Act as Inhibitory Constraints of Excitatory Synaptic Transmission Through Distinct Mechanisms. Scientific reports 27 30479371
2021 Tozasertib Attenuates Neuropathic Pain by Interfering with Aurora Kinase and KIF11 Mediated Nociception. ACS chemical neuroscience 26 34027667
2012 Receptor-ligand interaction-based virtual screening for novel Eg5/kinesin spindle protein inhibitors. Journal of medicinal chemistry 26 22309208
2009 KIF11 inhibition for glioblastoma treatment: reason to hope or a struggle with the brain? BMC cancer 26 19545421
2024 Mitotic Functions and Characters of KIF11 in Cancers. Biomolecules 25 38672404
2020 Enhancing Brain Retention of a KIF11 Inhibitor Significantly Improves its Efficacy in a Mouse Model of Glioblastoma. Scientific reports 25 32300151
2021 KIF11 inhibitors filanesib and ispinesib inhibit meningioma growth in vitro and in vivo. Cancer letters 24 33652084
2022 KIF11 As a Potential Pan-Cancer Immunological Biomarker Encompassing the Disease Staging, Prognoses, Tumor Microenvironment, and Therapeutic Responses. Oxidative medicine and cellular longevity 23 36742345
2015 Overexpression of Eg5 correlates with high grade astrocytic neoplasm. Journal of neuro-oncology 23 26456023
2018 Death receptor 6 promotes ovarian cancer cell migration through KIF11. FEBS open bio 22 30186750
2011 A potent chemotherapeutic strategy in prostate cancer: S-(methoxytrityl)-L-cysteine, a novel Eg5 inhibitor. Asian journal of andrology 22 21297652
2017 Biochemical and Biophysical characterization of curcumin binding to human mitotic kinesin Eg5: Insights into the inhibitory mechanism of curcumin on Eg5. International journal of biological macromolecules 21 29162464
2012 Discovery by the Epistasis Project of an epistatic interaction between the GSTM3 gene and the HHEX/IDE/KIF11 locus in the risk of Alzheimer's disease. Neurobiology of aging 21 23036584
2022 Update on the Phenotypic and Genotypic Spectrum of KIF11-Related Retinopathy. Genes 20 35456519
2018 The nonmotor adaptor HMMR dampens Eg5-mediated forces to preserve the kinetics and integrity of chromosome segregation. Molecular biology of the cell 20 29386294
2016 The kinesin Eg5 inhibitor K858 induces apoptosis but also survivin-related chemoresistance in breast cancer cells. Investigational new drugs 20 26994617
2014 Modulation of Eg5 activity contributes to mitotic spindle checkpoint activation and Tat-mediated apoptosis in CD4-positive T-lymphocytes. The Journal of pathology 20 24488929
2012 p63 expression correlates with sensitivity to the Eg5 inhibitor ZD4877 in bladder cancer cells. Cancer biology & therapy 20 22361733
2020 p21-activated kinase 6 controls mitosis and hepatocellular carcinoma progression by regulating Eg5. Biochimica et biophysica acta. Molecular cell research 19 33098954
2018 Non-canonical functions of the mitotic kinesin Eg5. Thoracic cancer 19 29927078
2012 Inhibition of Eg5 acts synergistically with checkpoint abrogation in promoting mitotic catastrophe. Molecular cancer research : MCR 19 22522457
2012 The functional antagonism between Eg5 and dynein in spindle bipolarization is not compatible with a simple push-pull model. Cell reports 18 22832270
2010 Association between variants in IDE-KIF11-HHEX and plasma amyloid β levels. Neurobiology of aging 18 20724036
2021 Upregulation of KIF11 in TP53 Mutant Glioma Promotes Tumor Stemness and Drug Resistance. Cellular and molecular neurobiology 17 33491154
2021 KIF11 is upregulated in colorectal cancer and silencing of it impairs tumor growth and sensitizes colorectal cancer cells to oxaliplatin via p53/GSK3β signaling. Journal of Cancer 17 33995648
2019 MicroRNA-186-5p represses neuroblastoma cell growth via downregulation of Eg5. American journal of translational research 17 31105832
2018 Meiotic arrest and spindle defects are associated with altered KIF11 expression in porcine oocytes. Environmental and molecular mutagenesis 17 30151839
2014 Knockdown of kinesin KIF11 abrogates directed migration in response to epidermal growth factor-mediated chemotaxis. Biochemical and biophysical research communications 17 25193695
2009 Substituted benzimidazoles: A novel chemotype for small molecule hKSP inhibitors. Bioorganic & medicinal chemistry letters 17 19481450
2025 Identification and validation of the important role of KIF11 in the development and progression of endometrial cancer. Journal of translational medicine 16 39806429
2022 LncRNA VPS9D1-AS1 Promotes Malignant Progression of Lung Adenocarcinoma by Targeting miRNA-30a-5p/KIF11 Axis. Frontiers in genetics 16 35140744
2022 Using a comprehensive approach to investigate the interaction between Kinesin-5/Eg5 and the microtubule. Computational and structural biotechnology journal 16 36051882
2022 Kinesin-5 Eg5 is essential for spindle assembly, chromosome stability and organogenesis in development. Cell death discovery 16 36513626
2021 High KIF11 expression is associated with poor outcome of NSCLC. Tumori 16 33554761
2021 ASPM combined with KIF11 promotes the malignant progression of hepatocellular carcinoma via the Wnt/β-catenin signaling pathway. Experimental and therapeutic medicine 16 34504599
2017 Protein Phosphatase 2A (PP2A) Regulates EG5 to Control Mitotic Progression. Scientific reports 16 28487562
2017 The kinesin Eg5 inhibitor K858 induces apoptosis and reverses the malignant invasive phenotype in human glioblastoma cells. Investigational new drugs 16 28965307
2016 In Vitro Maturation of Mouse Oocytes Increases the Level of Kif11/Eg5 on Meiosis II Spindles. Biology of reproduction 16 27146033
2008 Cloning, enzyme characterization of recombinant human Eg5 and the development of a new inhibitor. Biological & pharmaceutical bulletin 16 18591782
2023 QSAR-based virtual screening of traditional Chinese medicine for the identification of mitotic kinesin Eg5 inhibitors. Computational biology and chemistry 15 37062146
2023 In vitro antitumor activity, molecular dynamics simulation, DFT study, ADME prediction, and Eg5 binding of enastron analogues. RSC advances 15 37388149
2021 Retinal Features of Family Members With Familial Exudative Vitreoretinopathy Caused By Mutations in KIF11 Gene. Translational vision science & technology 15 34128965
2018 S-trityl-L-cysteine, a novel Eg5 inhibitor, is a potent chemotherapeutic strategy in neuroblastoma. Oncology letters 15 29963178
2024 Di-(2-ethylhexyl) phthalate promotes benign prostatic hyperplasia through KIF11-Wnt/β-catenin signaling pathway. Ecotoxicology and environmental safety 14 38944010
2023 Morphological Profiling Identifies the Motor Protein Eg5 as Cellular Target of Spirooxindoles. Angewandte Chemie (International ed. in English) 14 36929571
2020 Kinesin-5 Eg5 is essential for spindle assembly and chromosome alignment of mouse spermatocytes. Cell division 14 32165913
2018 KIF11 microdeletion is associated with microcephaly, chorioretinopathy and intellectual disability. Human genome variation 14 31428438
2017 CD52, CD22, CD26, EG5 and IGF-1R expression in thymic malignancies. Lung cancer (Amsterdam, Netherlands) 14 28625631