Affinage

MYO1D

Unconventional myosin-Id · UniProt O94832

Round 2 corrected
Length
1006 aa
Mass
116.2 kDa
Annotated
2026-04-29
85 papers in source corpus 22 papers cited in narrative 22 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

MYO1D is an unconventional class I myosin that couples actin-based motor activity to membrane dynamics, serving as a versatile linker between the actin cytoskeleton and cellular membranes in contexts ranging from vesicle trafficking to organ laterality. Its motor domain exhibits a high actin-activated ATPase rate with MgADP release as the rate-limiting step, enabling robust vesicle transport along actin filaments and driving clockwise F-actin organization that underlies dextral cell chirality (PMID:37380077, PMID:29769531, PMID:29478852). MYO1D anchors unphosphorylated EGFR family receptors at the plasma membrane to regulate receptor-level signaling, promotes PDGFRα/β heterodimer internalization into early endosomes to negatively regulate ERK signaling, localizes the initiator caspase Dronc to the cell cortex to drive apoptosis-induced compensatory proliferation, and directs rotational and translational planar cell polarity in ciliated epithelia through functional interaction with Vangl2 (PMID:31420606, PMID:40404618, PMID:36469525, PMID:26446290). MYO1D also supports oligodendrocyte myelin membrane maintenance and remyelination in the CNS, and cooperates with Nesprin1 for actin-dependent mRNA transport to postsynaptic sites at the neuromuscular junction (PMID:27655972, PMID:28986688, PMID:25959729).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 2000 High

    Establishing MYO1D as a membrane-trafficking motor: prior to this work it was unknown whether class I myosins participated in endosomal sorting; reconstituted assays demonstrated that Myr4/MYO1D, with calmodulin as its light chain, is required for actin-dependent transfer of cargo from early endosomes to recycling endosomes.

    Evidence In vitro endosome transfer assay in MDCK cells with calmodulin inhibition and actin polymerization perturbation

    PMID:11208135

    Open questions at the time
    • No structural basis for how MYO1D selects recycling endosome membranes
    • Whether this trafficking role extends to non-epithelial cells was untested
  2. 2012 Medium

    Expanding MYO1D's tissue repertoire to the nervous system: MYO1D was found in axonal and myelin compartments, interacting with aspartoacylase (ASPA), suggesting a previously unrecognized role in myelination-related lipid metabolism.

    Evidence Co-immunoprecipitation of MYO1D with ASPA, immunofluorescence in mouse sciatic nerve and cerebellum

    PMID:22284616

    Open questions at the time
    • Single Co-IP for ASPA interaction without reciprocal validation
    • Functional consequence of MYO1D-ASPA interaction untested
    • No loss-of-function data in this study
  3. 2015 High

    Demonstrating MYO1D as a planar cell polarity effector: knockout rats revealed that MYO1D is required for unidirectional basal body alignment and Vangl1 polarization in multi-ciliated epithelia, resolving how actin-based motors contribute to rotational and translational PCP.

    Evidence Myo1d knockout rat with SEM/TEM, Vangl1 immunofluorescence, and bead transport assay in tracheal and ependymal epithelia

    PMID:26446290

    Open questions at the time
    • Whether MYO1D directly binds PCP core components or acts indirectly was unresolved
    • Mechanism linking motor activity to basal body rotation unknown
  4. 2015 High

    Revealing a postsynaptic mRNA transport function: MYO1D and Nesprin1 were shown to be mutually dependent for localization to immature boutons and required for actin-based mRNA delivery to postsynaptic sites at the Drosophila NMJ, establishing a motor-scaffold partnership for local translation.

    Evidence Genetic loss-of-function in Drosophila, mRNA localization assays, immunofluorescence at NMJ

    PMID:25959729

    Open questions at the time
    • Identity of transported mRNAs not fully catalogued
    • Whether this mechanism operates at mammalian synapses unknown
  5. 2016 Medium

    Confirming a cell-autonomous role in oligodendrocyte survival and myelin membrane dynamics: MYO1D knockdown caused process retraction, impaired PLP transport, and apoptosis in oligodendrocytes, directly linking MYO1D motor function to myelin sheath integrity.

    Evidence siRNA knockdown in primary rat oligodendrocyte cultures with live-cell morphology and PLP trafficking analysis

    PMID:27655972

    Open questions at the time
    • Whether the apoptosis is a direct consequence of PLP mistrafficking or a parallel effect was unclear
    • No rescue experiment reported
  6. 2017 Medium

    Extending MYO1D's myelin role in vivo: stereotaxic knockdown during cuprizone-induced demyelination impaired remyelination and shifted glial inflammatory profiles, establishing MYO1D as required for CNS repair.

    Evidence In vivo siRNA injection in corpus callosum, cuprizone model, immunohistochemistry

    PMID:28986688

    Open questions at the time
    • siRNA specificity controls not fully detailed
    • Whether the inflammatory shift is cell-autonomous to oligodendrocytes or secondary was unresolved
  7. 2018 High

    Establishing MYO1D as a conserved determinant of vertebrate left-right asymmetry: morpholino knockdown in both zebrafish and Xenopus disrupted LR organizer cilia orientation, Nodal cascade activation, and organ situs, with genetic epistasis placing MYO1D in the PCP (Vangl2) pathway.

    Evidence Morpholino knockdown in zebrafish and Xenopus with cilia imaging, fluid flow analysis, in situ hybridization, convergent extension assays, and Vangl2 genetic interaction

    PMID:29478852 PMID:29769531

    Open questions at the time
    • Whether MYO1D physically binds Vangl2 or acts in parallel was unresolved
    • The intrinsic chirality mechanism of MYO1D motor was not yet characterized
  8. 2019 High

    Identifying MYO1D as a direct membrane anchor for EGFR family receptors: the β-meander motif in MYO1D's tail was shown to bind EGFR kinase domains and stabilize receptors at the plasma membrane, with overexpression promoting tumor progression via upregulated EGFR signaling.

    Evidence Reciprocal Co-IP, domain mapping, syngeneic mouse tumor model, cell viability/motility assays in colorectal and breast cancer lines

    PMID:31420606

    Open questions at the time
    • Whether MYO1D-EGFR interaction occurs in normal physiology or only in overexpression contexts
    • Structural basis of the β-meander–kinase domain interface not resolved
  9. 2020 High

    Defining MYO1D's role in non-apoptotic caspase signaling: MYO1D-dependent membrane localization of Dronc in mature enterocytes was shown to drive ROS, hemocyte recruitment, and JNK signaling that sustains homeostatic intestinal stem cell proliferation.

    Evidence Drosophila Myo1D mutant midgut analysis with ROS, JNK reporters, hemocyte imaging

    PMID:33238125

    Open questions at the time
    • How MYO1D physically tethers Dronc to the membrane was not determined
    • Whether this mechanism operates in mammalian intestinal stem cells unknown
  10. 2022 High

    Placing MYO1D in the Dronc–LIMK1–F-actin–ROS–JNK cascade: genetic epistasis showed that MYO1D is required for the synergistic F-actin accumulation and ROS/JNK activation driven by co-expressed Dronc and LIMK1 during apoptosis-induced proliferation.

    Evidence Drosophila genetic epistasis with myo1d, limk1, dronc; F-actin staining, ROS and JNK reporter assays

    PMID:36469525

    Open questions at the time
    • Direct biochemical interaction between MYO1D and LIMK1 not shown
    • Whether MYO1D motor activity or only membrane tethering is required was untested
  11. 2022 Medium

    Connecting MYO1D to EGFR signaling through SPAG6: SPAG6 was identified as an interactor that promotes MYO1D translocation to the plasma membrane, upregulating EGFR family expression and PI3K/AKT and ERK signaling in AML cells.

    Evidence Co-IP/mass spectrometry, subcellular fractionation, xenograft mouse model in AML cells

    PMID:35667090

    Open questions at the time
    • Whether SPAG6 directly binds MYO1D or acts through an intermediary not fully resolved
    • Single-lab finding awaiting independent confirmation
  12. 2023 High

    Characterizing MYO1D's intrinsic motor properties: reconstituted kinetics revealed MYO1D has a 12.5-fold higher ATPase rate than MYO1C, with MgADP release as its rate-limiting step, and demonstrated robust vesicle transport and clockwise actin gliding consistent with a fast transport motor that could underlie dextral chirality.

    Evidence Steady-state ATPase, stopped-flow transient kinetics, in vitro actin gliding and liposome transport assays with Drosophila MYO1D

    PMID:37380077

    Open questions at the time
    • Whether mammalian MYO1D shares identical kinetic properties not confirmed
    • Structural basis for clockwise vs. counterclockwise movement not resolved
  13. 2025 High

    Revealing MYO1D as a negative regulator of PDGFRα/β heterodimer signaling: MYO1D preferentially binds and internalizes PDGFRα/β heterodimers; its loss retains receptors at the plasma membrane, amplifying ERK1/2 signaling and proliferation — establishing a receptor-trafficking function opposite to its EGFR-stabilizing role.

    Evidence Bimolecular fluorescence complementation, AP-MS, siRNA knockdown with phospho-ERK1/2 and proliferation readouts

    PMID:40404618

    Open questions at the time
    • How MYO1D distinguishes PDGFR heterodimers from homodimers mechanistically unclear
    • Whether this trafficking role involves the same tail domain β-meander used for EGFR binding untested
  14. 2025 Medium

    Demonstrating that MYO1D ensures survival of apoptosis-resistant cells during tissue regeneration: without MYO1D, DARE cells in irradiated wing discs undergo lethal effector caspase activation, disrupting compensatory proliferation and regeneration.

    Evidence Drosophila Myo1D loss-of-function in wing imaginal discs, delayed Dronc reporter, effector caspase assays

    PMID:41345088

    Open questions at the time
    • Precise mechanism by which MYO1D prevents effector caspase activation not fully resolved
    • Whether this anti-apoptotic function depends on Dronc membrane tethering not directly shown

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structural basis of MYO1D's intrinsic chirality, whether its EGFR-anchoring and PDGFR-internalizing roles use the same or distinct tail-domain interfaces, how MYO1D physically tethers Dronc to the plasma membrane, and whether the PCP-related and chirality functions are conserved in mammals.
  • No crystal structure of MYO1D tail domain in complex with any receptor
  • No mammalian loss-of-function model for left-right asymmetry
  • Direct MYO1D–Dronc binding mechanism unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003774 cytoskeletal motor activity 4 GO:0008092 cytoskeletal protein binding 4 GO:0140657 ATP-dependent activity 2
Localization
GO:0005886 plasma membrane 7 GO:0005856 cytoskeleton 5 GO:0005929 cilium 3 GO:0005768 endosome 2 GO:0005829 cytosol 1
Pathway
R-HSA-162582 Signal Transduction 5 R-HSA-5357801 Programmed Cell Death 3 R-HSA-5653656 Vesicle-mediated transport 3 R-HSA-1266738 Developmental Biology 2
Partners
ASPAEGFRERBB2ERBB4NESPRIN1PDGFRASPAG6VANGL2

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 Myr4 (the rat ortholog of MYO1D) functions as an actin-based mechanoenzyme required for membrane trafficking along the recycling endosome pathway in MDCK epithelial cells; calmodulin serves as its light chain, and polymerized actin is necessary for transfer of endocytosed markers from early endosomes to recycling endosomes. In vitro endosome transfer assay, calmodulin inhibition, actin polymerization perturbation, identification of myr4 as CaM target Traffic High 11208135
2002 Myo1d (myosin-Ig/myr4) is expressed in rodent auditory and vestibular epithelia but at lower levels than Myo1b, Myo1c, and Myo1e; confirmed by RT-PCR with isozyme-selective amplification. RT-PCR with isozyme-selective primers in neonatal rodent inner ear epithelia Journal of the Association for Research in Otolaryngology Medium 12486594
2009 Xenopus Myo1d protein is present from egg stages with increased expression at neurula through tadpole stages, and its mRNA is localized to neural tube, pre-somitic mesoderm, somites, and cranial neural crest cells during migration; a novel isoform-specific tail homology embedded in the TH1 domain was identified by sequence analysis. Western blot, whole-mount in situ hybridization, cDNA cloning and sequence analysis Development, growth & differentiation Medium 19382939
2012 Myosin-1d is expressed in axons and myelin compartments of mouse sciatic nerve, in Purkinje and granule cell layers of cerebellum, and expands along axonal tracts upon myelination onset; myosin-1d interacts with and is co-expressed with aspartoacylase (ASPA), an enzyme central to fatty acid synthesis in the nervous system. Immunofluorescence, co-immunoprecipitation (interaction with ASPA), Western blot across developmental stages Brain research Medium 22284616
2014 Myo1d is expressed in myelinating oligodendrocytes of rat CNS, particularly enriched in the outer (abaxonal) and inner (adaxonal) cytoplasm-containing loops of myelin; expression increases during CNS white matter maturation and is low in PNS myelin. Immunofluorescence with isoform-specific antibody, Western blot during CNS development, cell culture Journal of neuroscience research Medium 24903835
2015 In Drosophila, Myo1D functions with Nesprin1 (dNesp1) to transport mRNAs from muscle nuclei to postsynaptic sites at the neuromuscular junction along F-actin-based 'railroad track' filaments; dNesp1 and Myo1D are mutually required for their localization to immature boutons, and loss of dNesp1 prevents mRNA localization and synaptic maturation. Genetic loss-of-function (dNesp1 mutants), live imaging, immunofluorescence, mRNA localization assays in Drosophila NMJ Neuron High 25959729
2015 Myo1d knock-out rats show disrupted rotational planar cell polarity (PCP) in tracheal multi-ciliated epithelial cells: ciliary basal bodies lose unidirectional alignment (cilia splay radially), the asymmetric localization of PCP core protein Vangl1 is lost, and cilia-driven bead transport is impaired in velocity and linearity. In brain ependymal cells, left-right positioning of basal body clusters is lost, disrupting translational PCP. Rat knockout (Myo1d KO), scanning/transmission electron microscopy, immunofluorescence for Vangl1, bead transport assay Cytoskeleton High 26446290
2016 siRNA-mediated knockdown of Myo1d in cultured oligodendrocytes causes retraction of processes, degeneration of myelin-like membrane, apoptosis, and impaired intracellular PLP (proteolipid protein) transport; Myo1d co-localizes with actin filaments, CNPase, and partially with PLP at the leading edge of myelin-like membrane in differentiated OLs. siRNA knockdown in primary rat oligodendrocyte cultures, immunofluorescence, live-cell morphology analysis ASN Neuro Medium 27655972
2017 In a cuprizone-induced demyelination mouse model, stereotaxic siRNA knockdown of Myo1d in corpus callosum decreases MBP and PLP immunofluorescence intensity during remyelination, increases pro-inflammatory microglia and astrocytes, decreases anti-inflammatory microglia, and increases caspase-3-positive oligodendrocytes, indicating Myo1d is required for successful remyelination in vivo. In vivo siRNA injection (stereotaxic), cuprizone demyelination model, immunohistochemistry, cell counting Neurochemical research Medium 28986688
2018 Myosin1D (Myo1D) is an evolutionarily conserved regulator of left-right asymmetry in zebrafish: it is required for formation and function of Kupffer's vesicle (KV, the LR organizer), controls orientation of KV cilia, and interacts functionally with the PCP pathway component VanGogh-like2 (Vangl2) to establish productive LRO flow and organ laterality. Zebrafish myo1d morpholino knockdown, ciliary imaging, flow analysis, genetic interaction with Vangl2 morphants Nature communications High 29769531
2018 Morpholino-mediated myo1d downregulation in Xenopus disrupts organ placement, impairs Nodal cascade induction, compromises dand5 expression and LRO cilia (shorter, fewer, non-polarized), inhibits convergent extension, and downregulates a non-canonical Wnt (ATF2) reporter; genetic interference shows functional interaction between vangl2 and myo1d in LR axis formation, placing Myo1d in the PCP pathway. Xenopus morpholino knockdown, in situ hybridization, cilia analysis, convergent extension assay, ATF2 reporter, vangl2 genetic interaction Current Biology High 29478852
2019 MYO1D functions to anchor unphosphorylated EGFR family members (EGFR, ErbB2, ErbB4 but not ErbB3) to the plasma membrane via direct binding between the β-meander motif in MYO1D's C-terminal tail domain and the kinase domains of EGFRs; expression of the tail domain alone suppresses the oncogenic action of full-length MYO1D. Overexpression of MYO1D increases colorectal and breast cancer cell motility and viability by upregulating EGFR levels and promotes colorectal tumor progression in a syngeneic mouse model. Co-immunoprecipitation, co-localization, domain mapping (tail domain expression), syngeneic mouse tumor model, cell viability/motility assays Oncogene High 31420606
2020 In Drosophila, Myo1D-dependent localization of the initiator caspase Dronc to the plasma membrane of mature enterocytes (ECs) is required for homeostatic intestinal stem cell (ISC) activity; in Myo1D mutant midguts, ROS generation, hemocyte recruitment, and JNK signaling are all reduced, indicating Myo1D-dependent Dronc membrane localization in ECs drives the signals that stimulate ISC mitosis. Drosophila Myo1D mutant analysis, live imaging of midgut, ROS assay, JNK signaling reporters, hemocyte recruitment assay Cell Reports High 33238125
2022 In Drosophila apoptosis-induced proliferation (AiP), Myo1D localizes the initiator caspase Dronc to the cell cortex; F-actin accumulates in a Dronc-dependent manner and drives ROS production and JNK activation. LIMK1 promotes F-actin polymerization downstream of Dronc, and the synergistic effects of co-expressed Dronc and LIMK1 on F-actin accumulation, ROS, and JNK activation require Myo1D, placing Myo1D as essential for cortical Dronc-driven AiP signaling. Drosophila genetic epistasis (myo1d, limk1, dronc), ROS assay, JNK reporter, F-actin staining, co-expression experiments PLoS Genetics High 36469525
2022 SPAG6 interacts with MYO1D (shown by immunoprecipitation and mass spectrometry) and overexpression of SPAG6 promotes translocation of MYO1D from cytosol to the cell membrane, thereby upregulating EGFR family expression and activating PI3K/AKT and ERK signaling to promote AML cell proliferation and migration. Co-immunoprecipitation, mass spectrometry, subcellular fractionation, flow cytometry, xenograft mouse model Blood Advances Medium 35667090
2022 miR-217-5p directly targets Myo1d (validated by luciferase assay, qRT-PCR, and Western blot), and its overexpression in podocytes causes shrunken cells with abnormal actin cytoskeletons, suggesting Myo1d plays a role in podocyte morphogenesis and actin organization. Luciferase reporter assay, qRT-PCR, Western blot, miRNA overexpression in E11 podocyte cells Non-coding RNA Medium 35736640
2023 Drosophila Myo1D has a 12.5-fold higher actin-activated steady-state ATPase rate than Myo1C, an 8-fold higher MgADP release rate, and MgADP release (not phosphate release) is the rate-limiting step for Myo1D, whereas phosphate release is rate-limiting for Myo1C. Both myosins have among the tightest MgADP affinities measured for any myosin. Myo1D propels actin filaments at higher speeds and robustly transports 50 nm unilamellar vesicles along actin filaments in vitro, consistent with a transport motor role. Steady-state ATPase assay, transient kinetics (stopped-flow), in vitro actin gliding assay, liposome transport assay The Journal of Biological Chemistry High 37380077
2023 KITENIN complex component MYO1D is required for KITENIN-mediated aerobic glycolysis in colorectal cancer cells; silencing MYO1D downregulates glycolytic proton efflux rate and expression of glycolytic enzymes (GLUT1, HK2, PKM2, LDHA). siRNA knockdown of MYO1D, glycolytic flux assay (Seahorse), protein expression analysis Cell & Bioscience Medium 37553596
2024 Single-molecule imaging in Drosophila macrophages shows Myo1D exhibits a significantly smaller slow-diffusing population on the plasma membrane compared to Myo1C; this difference is contingent on both head and tail domains of Myo1C and the two myosins do not mutually influence each other's membrane diffusion, suggesting distinct membrane interaction mechanisms underlie their opposite chirality-determining activities. Single-molecule imaging (TIRF/PALM) in live Drosophila macrophages, diffusion coefficient analysis Genes to Cells Medium 38454557
2025 MYO1D preferentially binds PDGFRα/β heterodimers and promotes their internalization from the plasma membrane into early endosomes; knockdown of MYO1D causes retention of PDGFRα/β heterodimers at the plasma membrane, leading to increased ERK1/2 phosphorylation and increased cell proliferation, identifying MYO1D as a negative regulator of PDGFRα/β heterodimer signaling through receptor trafficking. Bimolecular fluorescence complementation, AP-MS interactome, siRNA knockdown, phospho-ERK1/2 immunoblot, cell proliferation assay Nature Communications High 40404618
2025 Myo1D in Drosophila macrophages directs clockwise circumferential F-actin flow, while Myo1C directs counterclockwise flow; both depend on Myosin2 (Myo2). In a modified in vitro motility assay at near-physiological actin concentrations, Myo1D triggers self-organization of a clockwise-rotating chiral F-actin ring, implying Myo1D organizes parallelly polarized (barbed-end-biased) F-actin bundles to drive dextral cell chirality through Myo2-driven circumferential flow. Live-cell F-actin flow imaging in Drosophila macrophages, modified in vitro motility assay, genetic perturbation of Myo2 bioRxivpreprint Medium bio_10.1101_2025.05.06.648335
2025 In Drosophila wing disc regeneration after ionizing radiation, Myo1D ensures survival of Dronc-activating apoptosis-resistant (DARE) cells by preventing lethal effector caspase activation; without Myo1D, DARE cells undergo apoptosis, disrupting compensatory proliferation and tissue regeneration. Drosophila Myo1D genetic loss-of-function in wing imaginal discs, delayed Dronc reporter, effector caspase assays, proliferation quantification Nature Communications Medium 41345088

Source papers

Stage 0 corpus · 85 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2007 Large-scale mapping of human protein-protein interactions by mass spectrometry. Molecular systems biology 733 17353931
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2008 Large-scale proteomics and phosphoproteomics of urinary exosomes. Journal of the American Society of Nephrology : JASN 607 19056867
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2005 Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Genome research 409 16344560
2013 Genome-wide meta-analyses of multiancestry cohorts identify multiple new susceptibility loci for refractive error and myopia. Nature genetics 387 23396134
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2010 Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics. Cell 318 21145461
2009 Docking motif-guided mapping of the interactome of protein phosphatase-1. Chemistry & biology 269 19389623
2009 Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). Journal of proteome research 237 19199708
2015 ∆F508 CFTR interactome remodelling promotes rescue of cystic fibrosis. Nature 209 26618866
2018 An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations. Nature communications 201 29568061
2020 UFMylation maintains tumour suppressor p53 stability by antagonizing its ubiquitination. Nature cell biology 168 32807901
2009 Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. American journal of human genetics 164 19913121
2009 Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia. European archives of psychiatry and clinical neuroscience 159 19165527
2020 AMPK, a Regulator of Metabolism and Autophagy, Is Activated by Lysosomal Damage via a Novel Galectin-Directed Ubiquitin Signal Transduction System. Molecular cell 152 31995728
2020 A High-Density Human Mitochondrial Proximity Interaction Network. Cell metabolism 148 32877691
2012 Functional proteomics establishes the interaction of SIRT7 with chromatin remodeling complexes and expands its role in regulation of RNA polymerase I transcription. Molecular & cellular proteomics : MCP 145 22586326
2022 A comprehensive SARS-CoV-2-human protein-protein interactome reveals COVID-19 pathobiology and potential host therapeutic targets. Nature biotechnology 140 36217030
2013 In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine. Proteomics 138 23533145
2019 Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms. Nature cell biology 137 31871319
2009 Charting the molecular network of the drug target Bcr-Abl. Proceedings of the National Academy of Sciences of the United States of America 137 19380743
2013 Proteomic analysis of podocyte exosome-enriched fraction from normal human urine. Journal of proteomics 126 23376485
2022 Human transcription factor protein interaction networks. Nature communications 123 35140242
2017 Global analysis of H3K27me3 as an epigenetic marker in prostate cancer progression. BMC cancer 83 28403887
2014 Genome-wide association identifies regulatory Loci associated with distinct local histogram emphysema patterns. American journal of respiratory and critical care medicine 71 25006744
2000 Both calmodulin and the unconventional myosin Myr4 regulate membrane trafficking along the recycling pathway of MDCK cells. Traffic (Copenhagen, Denmark) 67 11208135
2010 Myosin 1G is an abundant class I myosin in lymphocytes whose localization at the plasma membrane depends on its ancient divergent pleckstrin homology (PH) domain (Myo1PH). The Journal of biological chemistry 60 20071333
2018 Myosin1D is an evolutionarily conserved regulator of animal left-right asymmetry. Nature communications 57 29769531
2015 Comprehensive profiling of novel microRNA-9 targets and a tumor suppressor role of microRNA-9 via targeting IGF2BP1 in hepatocellular carcinoma. Oncotarget 56 26547929
2002 Myosin-I isozymes in neonatal rodent auditory and vestibular epithelia. Journal of the Association for Research in Otolaryngology : JARO 55 12486594
2020 Coimmunoprecipitation with MYR1 Identifies Three Additional Proteins within the Toxoplasma gondii Parasitophorous Vacuole Required for Translocation of Dense Granule Effectors into Host Cells. mSphere 51 32075880
2007 High density SNP association study of a major autism linkage region on chromosome 17. Human molecular genetics 45 17376794
2018 A Conserved Role of the Unconventional Myosin 1d in Laterality Determination. Current biology : CB 39 29478852
2015 Coding and noncoding expression patterns associated with rare obesity-related disorders: Prader-Willi and Alström syndromes. Advances in genomics and genetics 30 25705109
2015 Myosin Id is required for planar cell polarity in ciliated tracheal and ependymal epithelial cells. Cytoskeleton (Hoboken, N.J.) 30 26446290
2021 Conception by fertility treatment and offspring deoxyribonucleic acid methylation. Fertility and sterility 29 33823999
2016 Selective localization of myosin-I proteins in macropinosomes and actin waves. Cytoskeleton (Hoboken, N.J.) 28 26801966
2015 Nucleus to Synapse Nesprin1 Railroad Tracks Direct Synapse Maturation through RNA Localization. Neuron 25 25959729
2022 Actin remodeling mediates ROS production and JNK activation to drive apoptosis-induced proliferation. PLoS genetics 24 36469525
2019 MYO1D binds with kinase domain of the EGFR family to anchor them to plasma membrane before their activation and contributes carcinogenesis. Oncogene 24 31420606
2012 Expression and localization of myosin-1d in the developing nervous system. Brain research 22 22284616
2012 Genome-wide DNA methylation profiling of CpG islands in hypospadias. The Journal of urology 21 22906644
2020 Transiently "Undead" Enterocytes Mediate Homeostatic Tissue Turnover in the Adult Drosophila Midgut. Cell reports 20 33238125
2007 Mapping of a novel type III variant of Knobloch syndrome (KNO3) to chromosome 17q11.2. American journal of medical genetics. Part A 15 17975799
2017 Unconventional Myosin ID is Involved in Remyelination After Cuprizone-Induced Demyelination. Neurochemical research 14 28986688
2023 KITENIN promotes aerobic glycolysis through PKM2 induction by upregulating the c-Myc/hnRNPs axis in colorectal cancer. Cell & bioscience 13 37553596
2022 Upregulated SPAG6 promotes acute myeloid leukemia progression through MYO1D that regulates the EGFR family expression. Blood advances 13 35667090
2013 Transcriptome analysis of Inbred Long Sleep and Inbred Short Sleep mice. Genes, brain, and behavior 13 23433184
2014 Unconventional myosin ID is expressed in myelinating oligodendrocytes. Journal of neuroscience research 11 24903835
2021 Novel MYO1D Missense Variant Identified Through Whole Exome Sequencing and Computational Biology Analysis Expands the Spectrum of Causal Genes of Laterality Defects. Frontiers in medicine 10 34589502
2016 Knockdown of Unconventional Myosin ID Expression Induced Morphological Change in Oligodendrocytes. ASN neuro 10 27655972
2022 Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics. Translational oncology 9 35303583
2019 Basic-hydrophobic sites are localized in conserved positions inside and outside of PH domains and affect localization of Dictyostelium myosin 1s. Molecular biology of the cell 9 31774725
2023 Drosophila class-I myosins that can impact left-right asymmetry have distinct ATPase kinetics. The Journal of biological chemistry 8 37380077
2020 Proteome alterations associated with the oleic acid and cis-9, trans-11 conjugated linoleic acid content in bovine skeletal muscle. Journal of proteomics 8 32335295
2025 PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation. Nature communications 4 40404618
2023 Quantification of hematopoietic stem and progenitor cells by targeted DNA methylation analysis. Clinical epigenetics 4 37370186
2020 Dictyostelium myosin 1F and myosin 1E inhibit actin waves in a lipid-binding-dependent and motor-independent manner. Cytoskeleton (Hoboken, N.J.) 4 32734648
2018 An Interstitial 17q11.2 de novo Deletion Involving the CDK5R1 Gene in a High-Functioning Autistic Patient. Molecular syndromology 4 30733659
2017 Expression of Unconventional Myosin VI in Oligodendrocytes. Neurochemical research 4 28828543
2015 Isobaric Tags for Relative and Absolute Quantitation-Based Proteomic Analysis of Patent and Constricted Ductus Arteriosus Tissues Confirms the Systemic Regulation of Ductus Arteriosus Closure. Journal of cardiovascular pharmacology 4 25915513
2010 Using osteoclast differentiation as a model for gene discovery in an undergraduate cell biology laboratory. Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology 4 21567867
2025 Conserved Phosphorylation of the Myosin1e TH1 Domain Impacts Membrane Association and Function in Yeast and Worms. Cytoskeleton (Hoboken, N.J.) 2 40205688
2025 Comparative Transcriptome Analysis Reveals the Role of the FST Gene in Goose Muscle Development. Animals : an open access journal from MDPI 2 41153936
2024 Myosin1G promotes Nodal signaling to control zebrafish left-right asymmetry. Nature communications 2 39095343
2022 The Role of miR-217-5p in the Puromycin Aminonucleoside-Induced Morphological Change of Podocytes. Non-coding RNA 2 35736640
2018 Genetic Determinants of Cerebral Arterial Adaptation to Flow-loading. Current neurovascular research 2 29998805
2025 Drone pupae extract enhances Hanwoo myosatellite cell function for cultivated meat production. Journal of animal science and technology 1 39974789
2025 Apoptosis-resistant cells drive compensatory proliferation via cell-autonomous and non-autonomous functions of the initiator caspase Dronc. Nature communications 1 41345088
2024 Left-right Myosin-Is, Myosin1C, and Myosin1D exhibit distinct single molecule behaviors on the plasma membrane of Drosophila macrophages. Genes to cells : devoted to molecular & cellular mechanisms 1 38454557
2024 The Initiator Caspase Dronc Drives Compensatory Proliferation of Apoptosis-Resistant Cells During Epithelial Tissue Regeneration After Ionizing Radiation. bioRxiv : the preprint server for biology 1 39282309
2023 Spatial transcriptome profiling uncovers metabolic regulation of left-right patterning. bioRxiv : the preprint server for biology 1 37131609
2023 PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation. bioRxiv : the preprint server for biology 1 38234806
2009 Developmental expression of Xenopus myosin 1d and identification of a myo1d tail homology that overlaps TH1. Development, growth & differentiation 1 19382939
2025 The prevalence of laterality defects in patients with congenital heart disease. Journal of human genetics 0 40467998
2025 Knockoff-Based Fine Mapping of MS-Associated SNPs in Sardinian Trios. Biochemical genetics 0 40884622
2025 Integration of exosome-related genes and differential expression analysis reveals potential biomarkers for prostate cancer. Discover oncology 0 41186840
2014 Familial Lymphoproliferative Malignancies and Tandem Duplication of NF1 Gene. Case reports in oncological medicine 0 25580325