- Length
- 1136 aa
- Mass
- 132.0 kDa
- Annotated
- 2026-06-13
30 papers in source corpus
13 papers cited in narrative
14 extracted findings
Mechanistic narrative
Synthesis pass · prose summary of the discoveries below
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Mechanism profile
Synthesis pass · controlled-vocabulary classification · explore literature graph →
No controlled-vocabulary terms were assigned to this entry.
Evidence
Reading pass · 14 per-paper findings extracted from the source corpus
| Year | Finding | Method | Journal | Conf | PMIDs |
|---|---|---|---|---|---|
| 1999 | MI(130)/MYO1B (myr-1 gene product) from rat liver has slow ATP-induced dissociation of actin-MI(130) (second-order rate constant ~1.7×10⁴ M⁻¹s⁻¹), slow ADP release from actin-MI(130) (~2 s⁻¹), ~10 µM affinity for ADP at actin-MI(130), ~15 nM affinity for actin, and Ca²⁺-sensitive ATPase activity (2-3-fold rate increase with CaCl₂), indicating MYO1B is adapted for maintenance of cortical tension rather than fast translocation. | Steady-state and stopped-flow transient kinetic analyses of purified rat liver MI(130)/MYO1B; Mg²⁺-ATPase assays; fluorescence-based actin dissociation experiments | The Journal of biological chemistry | High | 10419463 |
| 2005 | Myo1b is alternatively spliced to yield isoforms with six (myo1b-a), five (myo1b-b), or four (myo1b-c) IQ motifs; calmodulin binds tightly to a subset of IQ motifs (Kd < 0.2 µM) and very weakly to others (Kd > 5 µM); in vitro motility rate is isoform-dependent and calmodulin-concentration-dependent; Ca²⁺-dependent changes in ATPase activity are due to Ca²⁺ binding to the calmodulin closest to the motor domain; the regulatory domain acts as a rigid lever arm upon calmodulin binding. | Recombinant expression of biotinylated myo1b splice isoforms; ATPase assays; in vitro actin-gliding motility assays; calmodulin-binding measurements | The Journal of biological chemistry | High | 16254000 |
| 2005 | Myo1b concentrates on endosomal and ruffling membranes, consistent with roles in membrane trafficking and dynamics. | Cell biological localization studies of myo1b isoforms (implied by expression of isoforms and prior literature cited in this paper) | The Journal of biological chemistry | Medium | 16254000 |
| 2019 | SRSF1 promotes alternative splicing of MYO1B pre-mRNA, switching expression toward the full-length membrane-localized oncogenic isoform MYO1B-fl in glioma; this isoform switch activates the PDK1/AKT and PAK/LIMK pathways to drive cell proliferation, survival, and invasion. | RNA-Seq splicing analysis; SRSF1 knockdown/overexpression in glioma cells; isoform-specific functional assays (proliferation, invasion); pathway inhibitor experiments | The Journal of clinical investigation | Medium | 30481162 |
| 2017 | MYO1B mRNA is a direct target of miR-145-3p; siRNA-mediated knockdown of MYO1B in head and neck squamous cell carcinoma cells inhibited cancer cell proliferation, migration, and invasion, establishing MYO1B as a functionally relevant downstream effector. | miRNA target validation (direct regulation confirmed); siRNA knockdown of MYO1B; functional cell assays (proliferation, migration, invasion) | International journal of oncology | Medium | 29115582 |
| 2020 | Eucalyptal A downregulates SRSF1 expression and thereby corrects the SRSF1-guided abnormal alternative splicing of MYO1B mRNA, reducing MYO1B-fl isoform expression and exerting anti-glioblastoma activity through the PDK1/AKT/c-Myc and PAK/Cofilin axes. | Drug treatment of GBM cells; RT-PCR splicing analysis; western blotting of pathway components; in vitro proliferation/invasion assays; in vivo tumor-bearing mouse model | European journal of pharmacology | Medium | 33098832 |
| 2021 | MYO1B promotes F-actin rearrangement and focal adhesion assembly in colorectal cancer cells through activation of RhoA and the downstream ROCK2/LIMK/Cofilin axis, driving cell migration and invasion. | Lentiviral shRNA knockdown and overexpression; co-immunoprecipitation; western blotting; immunofluorescence of F-actin and focal adhesions; in vitro migration/invasion assays; in vivo xenograft | Annals of translational medicine | Medium | 34790749 |
| 2022 | Myo1b inhibits autophagosome-lysosome fusion in colorectal cancer cells, leading to accumulation of HIF-1α (by blocking its autophagic degradation), which in turn enhances VEGF secretion and promotes tumor angiogenesis. | Myo1b overexpression and siRNA knockdown in CRC cells; autophagy flux assays (autophagosome-lysosome fusion); HIF-1α protein stability assays; VEGF secretion ELISA; in vivo xenograft angiogenesis assessment | Cell death & disease | Medium | 36347835 |
| 2022 | MYO1B activates the SNAI2/cyclin D1 signaling pathway in esophageal squamous cell carcinoma; suppression of MYO1B downregulates SNAI2 expression, which in turn reduces cyclin D1 levels, and pharmacological inhibition of cyclin D1 activation reversed the aggressive phenotypes induced by SNAI2 overexpression in MYO1B-depleted cells. | siRNA knockdown; overexpression; qPCR and western blotting; functional assays (CCK8, colony formation, wound healing, Transwell); rescue experiments with cyclin D1 inhibitor | Journal of cellular physiology | Medium | 35861939 |
| 2023 | Myo1b interacts with LRRK2 through its tail domain, and this interaction promotes intracellular calcium elevation, which impairs autophagosome-lysosome fusion, leading to suppressed autophagic flux and promotion of endothelial cell senescence. | Co-immunoprecipitation of Myo1b and LRRK2; domain-mapping with tail domain constructs; intracellular calcium measurement; autophagy flux assays; Myo1b knockdown in senescent ECs and aged mice | Oxidative medicine and cellular longevity | Medium | 36654782 |
| 2024 | MYO1B interacts with classical swine fever virus Core (C) protein; Co-IP and co-localization experiments identified the N-terminus of the C protein as the key interaction site with MYO1B; MYO1B knockdown significantly reduced C protein and viral genome content in exosomes and decreased CSFV titers, demonstrating a role for MYO1B in intracellular transport of viral components to exosomes. | Co-immunoprecipitation; co-localization imaging; C protein point mutations and truncation variants; MYO1B knockdown by siRNA; viral titer measurement; exosome fractionation | Veterinary microbiology | Medium | 39603013 |
| 2025 | MYO1B and MYO5B (actin-based motor proteins) together with sorting nexin SNX27 regulate apical targeting and intracellular trafficking of membrane mucin MUC17 in enterocytes; specifically, MYO1B controls MUC17 protein levels and turnover at the brush border, whereas MYO5B governs MUC17 levels specifically at the brush border. | MYO1B and MYO5B knockdown/knockout in enterocyte models; MUC17 localization and turnover assays; biochemical fractionation | The Biochemical journal | Medium | 39661054 |
| 2025 | MYO1B knockdown in head and neck squamous cell carcinoma cells inhibited phosphorylation of ATM (DNA damage repair), reduced stemness markers (SOX2, OCT4), decreased EMT-related proteins, and increased radiotherapy sensitivity; these effects were mediated through the PI3K/AKT signaling pathway, as AKT activation reversed the reductions in tumor stemness and radioresistance. | siRNA knockdown; AKT activator rescue experiment; western blotting; in vitro functional assays; in vivo xenograft with irradiation | Cancer cell international | Medium | 40604770 |
| 2023 | MYO1B and MYO5B regulate the intracellular trafficking of membrane mucin MUC17 in enterocytes; MYO1B controls MUC17 protein levels and turnover at the brush border; loss of this trafficking function renders enterocytes sensitive to bacterial invasion. | MYO1B knockdown; MUC17 localization and turnover assays; bacterial challenge assays | bioRxivpreprint | Low | 36945389 |
Source papers
Stage 0 corpus · 30 papers · ranked by NIH iCite citations
| Year | Title | Journal | Citations | PMID |
|---|---|---|---|---|
| 2016 | A Novel Secreted Protein, MYR1, Is Central to Toxoplasma's Manipulation of Host Cells. | mBio | 133 | 26838724 |
| 2019 | Splicing factor SRSF1 promotes gliomagenesis via oncogenic splice-switching of MYO1B. | The Journal of clinical investigation | 123 | 30481162 |
| 1999 | Transient kinetic analysis of the 130-kDa myosin I (MYR-1 gene product) from rat liver. A myosin I designed for maintenance of tension? | The Journal of biological chemistry | 88 | 10419463 |
| 2017 | Passenger strand of miR-145-3p acts as a tumor-suppressor by targeting MYO1B in head and neck squamous cell carcinoma. | International journal of oncology | 62 | 29115582 |
| 2020 | Coimmunoprecipitation with MYR1 Identifies Three Additional Proteins within the Toxoplasma gondii Parasitophorous Vacuole Required for Translocation of Dense Granule Effectors into Host Cells. | mSphere | 53 | 32075880 |
| 2019 | Toxoplasma Controls Host Cyclin E Expression through the Use of a Novel MYR1-Dependent Effector Protein, HCE1. | mBio | 51 | 31040242 |
| 2018 | MYR1-Dependent Effectors Are the Major Drivers of a Host Cell's Early Response to Toxoplasma, Including Counteracting MYR1-Independent Effects. | mBio | 44 | 29615509 |
| 2005 | Biochemical and motile properties of Myo1b splice isoforms. | The Journal of biological chemistry | 43 | 16254000 |
| 2022 | Myo1b promotes tumor progression and angiogenesis by inhibiting autophagic degradation of HIF-1α in colorectal cancer. | Cell death & disease | 38 | 36347835 |
| 1999 | Functional expression and characterization of the myrosinase MYR1 from Brassica napus in Saccharomyces cerevisiae. | Protein expression and purification | 37 | 10600460 |
| 2021 | MYO1B enhances colorectal cancer metastasis by promoting the F-actin rearrangement and focal adhesion assembly via RhoA/ROCK/FAK signaling. | Annals of translational medicine | 27 | 34790749 |
| 2019 | The Virulence-Related MYR1 Protein of Toxoplasma gondii as a Novel DNA Vaccine Against Toxoplasmosis in Mice. | Frontiers in microbiology | 21 | 31024505 |
| 2022 | Targeting MYO1B impairs tumorigenesis via inhibiting the SNAI2/cyclin D1 signaling in esophageal squamous cell carcinoma. | Journal of cellular physiology | 11 | 35861939 |
| 2013 | Alternative splicing of Myb-related genes MYR1 and MYR2 may modulate activities through changes in dimerization, localization, or protein folding. | Plant signaling & behavior | 11 | 24309816 |
| 2023 | Myo1b Promotes Premature Endothelial Senescence and Dysfunction via Suppressing Autophagy: Implications for Vascular Aging. | Oxidative medicine and cellular longevity | 9 | 36654782 |
| 2020 | Eucalyptal A inhibits glioma by rectifying oncogenic splicing of MYO1B mRNA via suppressing SRSF1 expression. | European journal of pharmacology | 9 | 33098832 |
| 2016 | Analysis of structures and epitopes of a novel secreted protein MYR1 in Toxoplasma gondii. | Folia parasitologica | 9 | 27580381 |
| 2024 | AC024896.1/miR-363-3p Axis Regulates the Malignant Progression of Acute Myeloid Leukemia by Cuproptosis-Related Gene MYO1B. | Blood and lymphatic cancer : targets and therapy | 6 | 38550556 |
| 2008 | Characterization of MYR1, a dosage suppressor of YPT6 and RIC1 deficient mutants. | Current genetics | 5 | 18327588 |
| 2025 | MYO1B promotes radioresistance in head and neck squamous cell carcinoma by regulating tumor stemness and DNA damage repair via the PI3K/AKT pathway. | Cancer cell international | 3 | 40604770 |
| 2024 | Essential role of the interaction between classical swine fever virus core protein and cellular MYO1B in viral components transport to exosomes and titer maintenance. | Veterinary microbiology | 2 | 39603013 |
| 2024 | Toxoplasma gondii infection induces early host cell cycle arrest and DNA damage in primary human host cells by a MYR1-dependent mechanism. | Communications biology | 2 | 39681694 |
| 2023 | The MYO1B and MYO5B motor proteins and the SNX27 sorting nexin regulate membrane mucin MUC17 trafficking in enterocytes. | bioRxiv : the preprint server for biology | 2 | 36945389 |
| 2025 | The MYO1B and MYO5B motor proteins and the sorting nexin SNX27 regulate apical targeting of membrane mucin MUC17 in enterocytes. | The Biochemical journal | 1 | 39661054 |
| 2025 | Long noncoding RNA SNHG4 promotes glioma progression via regulating miR-367-3p/MYO1B axis in zebrafish xenografts. | Human cell | 1 | 39951205 |
| 2024 | Paracrine rescue of MYR1-deficient Toxoplasma gondii mutants reveals limitations of pooled in vivo CRISPR screens. | eLife | 1 | 39654402 |
| 2026 | Oncogenic role and potential mechanisms of MYO1B in breast cancer. | In vitro cellular & developmental biology. Animal | 0 | 41986768 |
| 2026 | Identification of Potential Biomarkers in Autism: PRELID2, MYO1B, LRCH2, LIFR, and RERG May Serve as Regulators in Synaptic Membrane-Integrating Interneurons. | Current gene therapy | 0 | 42227541 |
| 2025 | Molecular mechanisms of recombinant proteins PTTG1IP, ADAM12, PAPSS1, and MYO1B and their effects on wound repair induced by tendon exposure: Analysis of key genes. | International journal of biological macromolecules | 0 | 39978508 |
| 2025 | Formononetin suppresses osteosarcoma by targeting MYO1B and remodeling the tumor immune microenvironment. | APL bioengineering | 0 | 41488589 |
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