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Showing KIF23 — MKLP1 is a alias.

KIF23

Kinesin-like protein KIF23 · UniProt Q02241

Length
960 aa
Mass
110.1 kDa
Annotated
2026-06-10
81 papers in source corpus 35 papers cited in narrative 35 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

KIF23/MKLP1 is a kinesin-6 motor protein that drives the completion of cytokinesis by organizing the antiparallel microtubules of the anaphase central spindle and midbody (PMID:17942600). It assembles into the heterotetrameric centralspindlin complex with CYK-4/MgcRacGAP, and only the intact complex—not the individual subunits—bundles microtubules in vitro (PMID:17942600); this complex also coordinates spindle elongation with furrow ingression by excluding NuMA/dynein/dynactin from the equatorial membrane together with the RhoGEF Ect2 (PMID:36197340). MKLP1 is recruited to the spindle midzone and midbody via INCENP, a step required for midbody formation but not for chromosome segregation (PMID:15796717). Its activity is gated by a layered phosphoregulatory program: Aurora B phosphorylates conserved C-terminal serines to license cytokinesis completion (PMID:15854913), Plk1 binds through its Polo-box to the MKLP1 stalk and phosphorylates Ser904/Ser905, with MKLP1 in turn governing Plk1 localization in late mitosis (PMID:8524282, PMID:15199097), and sequential LATS/NDR phosphorylation at S716 then S814 creates a 14-3-3 binding site controlling MKLP1 clustering (PMID:25658096). The C-terminal tail mediates additional regulatory contacts, binding GTP-loaded Arf6 to form a membrane-apposed 2:2 heterotetramer required for abscission (PMID:10506747, PMID:22522702) and p120-catenin to spatially control RhoA cycling at the furrow (PMID:28004812); after abscission, TRAF6-mediated K63 ubiquitination marks the midbody-ring pool for clearance by selective autophagy (PMID:24128730). Beyond division, MKLP1 acts postmitotically to establish the nonuniform microtubule polarity of dendrites through its motor and tail domains (PMID:16418225) and to organize epithelial polarity (PMID:15182666), and it is required in the developing cortex for proper spindle orientation and progenitor cytokinesis (PMID:39632980). A missense KIF23 mutation (p.P916R) causes autosomal dominant congenital dyserythropoietic anemia type III through cytokinesis failure (PMID:23570799), and a microcephaly-associated variant fails to rescue cortical neurogenesis defects (PMID:39632980). In cancer, KIF23 is induced by FOXM1 and CUX1/E2F1 and repressed by the p53/p21/DREAM axis (PMID:23650552, PMID:19015243, PMID:36940637), and it promotes oncogenic Wnt/β-catenin signaling by sequestering Amer1 and stabilizing MYH9 to drive proliferation and drug resistance (PMID:32365332, PMID:41940421).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1995 Medium

    Established that MKLP1 is a physical and enzymatic target of a mitotic kinase, opening the question of how its cytokinetic function is regulated.

    Evidence Co-IP, co-localization, and in vitro kinase assay with Plk1 in mammalian cells

    PMID:8524282

    Open questions at the time
    • Phosphosites not mapped
    • Functional consequence of phosphorylation undefined
  2. 1998 Medium

    Showed MKLP1 has a postmitotic function in establishing mixed microtubule polarity, separating its role from cell division.

    Evidence Antisense depletion plus hook-decoration MT polarity assay in podocytes; expression profiling in neurons

    PMID:9749792 PMID:9864367

    Open questions at the time
    • Molecular mechanism of polarity establishment unresolved
    • Neuronal expression data correlative only
  3. 1999 Medium

    Identified the C-terminal tail as a regulatory/interaction module by mapping Arf binding and nuclear localization activity.

    Evidence Yeast two-hybrid, GST pulldown, Arf3 mutagenesis; GFP-fusion fractionation in HeLa

    PMID:10403813 PMID:10506747

    Open questions at the time
    • Physiological role of Arf binding not established here
    • Nuclear component bound by NLS unidentified
  4. 2004 High

    Defined the Plk1-MKLP1 interaction at domain and residue resolution and showed reciprocal dependence for cytokinesis, establishing MKLP1 as both Plk1 substrate and localization scaffold.

    Evidence Domain mapping, phosphosite mutagenesis (Ser904/905), RNAi depletion and rescue; C. elegans epithelial polarity genetics

    PMID:15182666 PMID:15199097

    Open questions at the time
    • How Ser904/905 phosphorylation alters molecular activity unclear
    • Link between Plk1 mislocalization and abscission failure mechanistic detail missing
  5. 2005 High

    Identified Aurora B as a cytokinesis-licensing kinase for MKLP1 and INCENP as its midzone/midbody recruitment factor, defining the recruitment-and-activation logic.

    Evidence In vitro kinase assay plus phospho-mutant analysis in C. elegans and human cells; RNAi plus 3D imaging for INCENP dependence

    PMID:15796717 PMID:15854913

    Open questions at the time
    • Precise C-terminal serines not all enumerated
    • How phosphorylation drives abscission completion not mechanistically resolved
  6. 2007 High

    Established the heterotetrameric architecture of centralspindlin and showed that complex assembly, not isolated subunits, confers microtubule-bundling, defining the minimal functional unit.

    Evidence Biochemical reconstitution, in vitro bundling assay, genetic suppressor screen, co-IP

    PMID:17942600

    Open questions at the time
    • Structural basis of bundling not determined
    • Regulation of assembly in cells incomplete
  7. 2012 High

    Provided a structural mechanism for membrane engagement at the cleavage furrow through the MKLP1-Arf6 heterotetramer.

    Evidence Crystal structure, structure-based mutagenesis, siRNA, live imaging

    PMID:22522702

    Open questions at the time
    • Coordination of Arf6 binding with motor activity unresolved
    • Upstream control of Arf6 GTP loading at furrow undefined
  8. 2013 High

    Connected MKLP1 to human disease and post-abscission turnover, showing a point mutation causes CDA III via cytokinesis failure and that TRAF6 ubiquitination targets the midbody ring for autophagy.

    Evidence Genetic identification plus RNAi rescue in HeLa (p.P916R); ubiquitination assay, co-IP, autophagy receptor analysis; p53/p21/DREAM transcriptional regulation; v-Src delocalization

    PMID:23562843 PMID:23570799 PMID:23650552 PMID:24128730

    Open questions at the time
    • How p.P916R perturbs molecular function unclear
    • Trigger for midbody-ring ubiquitination in normal cells undefined
  9. 2015 Medium

    Revealed a sequential LATS/NDR phosphorylation switch that creates a 14-3-3 binding site controlling MKLP1 clustering during cytokinesis.

    Evidence In vitro kinase assay, S716/S814 mutagenesis, in vivo phosphorylation, 14-3-3 binding assay

    PMID:25658096

    Open questions at the time
    • How clustering relates to bundling/abscission unresolved
    • Isoform-specificity of long CHO1 form in vivo unclear
  10. 2016 Medium

    Demonstrated MKLP1 partners with p120-catenin to spatially control RhoA cycling at the furrow and is transcriptionally limited in neurons by Toll-6/dSARM/FoxO signaling.

    Evidence Reciprocal co-IP, domain mapping, siRNA (p120); Drosophila genetic epistasis and MT-dynamics imaging (Toll-6/FoxO)

    PMID:27502486 PMID:28004812

    Open questions at the time
    • Mechanism by which MKLP1 modulates RhoA GAP/GEF activity unclear
    • Direct vs. indirect transcriptional control of MKLP1 by FoxO undefined
  11. 2022 Medium

    Extended centralspindlin function to membrane compartmentalization, showing it excludes NuMA/dynein/dynactin from the equatorial membrane to coordinate spindle elongation with furrowing; cancer studies linked KIF23 mRNA stability to oncogenic Wnt signaling.

    Evidence siRNA depletion plus live imaging (membrane exclusion); RIP-seq/acRIP-seq and xenografts for NAT10-mediated ac4C modification

    PMID:36197340 PMID:36522719

    Open questions at the time
    • Direct molecular basis of NuMA exclusion unresolved
    • Relationship between cytokinetic and oncogenic functions unclear
  12. 2024 High

    Established a developmental cortical requirement for KIF23 with allele-specific causality for microcephaly, and identified PTM-mediated stability control via SIRT7 desuccinylation.

    Evidence In utero electroporation knockdown plus rescue with WT vs. microcephaly variant; co-IP and site-specific succinylation assays (SIRT7/K537)

    PMID:38360598 PMID:39632980

    Open questions at the time
    • Whether cortical phenotype reflects cytokinesis vs. spindle orientation defects not fully separated
    • Generality of K537 succinylation across tissues unknown
  13. 2025 Medium

    Expanded KIF23 oncogenic and fibrotic signaling, linking it to MYH9 stabilization, RhoA/ROCK1-driven fibrosis, and transcriptional repression by ETV5.

    Evidence Co-IP, ubiquitination assays, CRISPR knockout (MYH9/USP7/MCM2); proteomics and in vivo shRNA (cardiac fibrosis); ChIP and AAV9 rescue (ETV5)

    PMID:40983152 PMID:41078122 PMID:41940421

    Open questions at the time
    • Whether motor activity is required for signaling functions unclear
    • Direct vs. scaffold roles in deubiquitinase recruitment not separated

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the layered phosphorylation, ubiquitination, and PTM inputs are integrated to time MKLP1 motor activity and centralspindlin assembly during abscission, and how its cytokinetic versus signaling roles are mechanistically partitioned, remain unresolved.
  • No unified structural model of regulated MKLP1 motor activation
  • Tail autoinhibition mechanism unestablished in reliable literature
  • Causal separation of cytokinetic and oncogenic signaling functions lacking

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 3 GO:0003774 cytoskeletal motor activity 2 GO:0008092 cytoskeletal protein binding 2
Localization
GO:0005634 nucleus 3 GO:0005856 cytoskeleton 2 GO:0005886 plasma membrane 2
Pathway
R-HSA-1640170 Cell Cycle 4 R-HSA-1266738 Developmental Biology 2 R-HSA-162582 Signal Transduction 2
Partners
AMER1ARF6AURKBCTNND1INCENPMYH9PLK1RACGAP1
Complex memberships
centralspindlin

Evidence

Reading pass · 35 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1995 PLK (Plk1) physically interacts with CHO1/MKLP-1 in vivo, co-localizes with it at the midbody during telophase/cytokinesis, and phosphorylates CHO1/MKLP-1 in vitro via Plk-associated kinase activity. Co-immunoprecipitation, co-localization by immunofluorescence, in vitro kinase assay Molecular and cellular biology Medium 8524282
2004 Plk1 binds CHO1/MKLP-1 through its Polo-box domain, while the stalk domain of CHO1/MKLP-1 mediates binding to Plk1. Ser904 and Ser905 are the two major Plk1 phosphorylation sites on CHO1/MKLP-1. Depletion of CHO1/MKLP-1 mislocalizes Plk1 during late mitosis. A non-phosphorylatable CHO1 mutant causes cytokinesis defects, and rescue of CHO1-depletion-induced multinucleation requires the phosphorylatable form. Transient transfection, domain-deletion analysis, vector-based RNAi, site-directed mutagenesis, rescue experiments Journal of cell science High 15199097
2005 Aurora B phosphorylates ZEN-4/MKLP1 in vitro and in vivo on conserved C-terminal serine residues. A non-phosphorylatable ZEN-4 mutant localizes properly but fails to support completion of cytokinesis in C. elegans embryos. Inhibition of aurora B in late anaphase in mammalian cells attenuates MKLP1 phosphorylation and causes cytokinesis defects without disrupting the central spindle. In vitro kinase assay, in vivo phosphorylation (C. elegans and human cells), phospho-mutant analysis, aurora kinase inhibitor treatment Current biology : CB High 15854913
2005 INCENP is required for recruiting MKLP1 to the spindle midzone/midbody. Depletion of MKLP1 by siRNA does not cause chromosome segregation or midzone formation defects, but abrogates midbody formation and completion of cytokinesis. INCENP-mediated recruitment of MKLP1 to the midzone/midbody is a crucial step for midbody formation. RNAi/siRNA knockdown, immunofluorescence, 3D live-cell imaging reconstruction The Biochemical journal Medium 15796717
2007 Centralspindlin is a heterotetrameric complex consisting of two CYK-4/MgcRacGAP homodimers and two ZEN-4/MKLP1 homodimers (each subunit dimerizing via parallel coiled coils) assembled through two low-affinity interactions. The assembled centralspindlin complex, but not individual subunits alone, is sufficient to bundle microtubules in vitro. Biochemical reconstitution, in vitro microtubule bundling assay, genetic epistasis (suppressor screen with second-site mutations), co-immunoprecipitation Molecular biology of the cell High 17942600
1999 Activated (GTP-bound) Arf proteins bind directly to an 88-amino-acid domain in the C-terminal tail of MKLP1. This interaction is GTP-dependent and maps to the switch I and switch II regions of Arf3. All human Arf isoforms interact with the MKLP1 C-terminal domain. Yeast two-hybrid screen, GST pulldown assay, deletion mapping, Arf3 point-mutation screen Cell motility and the cytoskeleton Medium 10506747
2012 Arf6 directly interacts with MKLP1 to form a 2:2 heterotetramer. Crystal structure reveals an extended β-sheet spanning the entire heterotetramer, suitable for interaction with concave membrane surfaces at the cleavage furrow. Arf6 first accumulates around the cleavage furrow, then is recruited to the Flemming body via MKLP1. Structure-based mutagenesis and siRNA knockdown show complex formation is required for completion of cytokinesis. Crystal structure determination, structure-based mutagenesis, siRNA knockdown, live-cell imaging The EMBO journal High 22522702
2013 TRAF6 mediates K63-linked ubiquitination of the midbody ring-localized protein KIF23/MKLP1. This ubiquitination is important for recognition by ubiquitin-binding autophagy receptors SQSTM1/p62 and NBR1 (together with WDFY3/ALFY) and subsequent degradation of midbody ring derivatives by selective autophagy. siRNA depletion, ubiquitination assay, co-immunoprecipitation, immunofluorescence Autophagy Medium 24128730
2013 A missense mutation c.2747C>G (p.P916R) in KIF23 causes autosomal dominant congenital dyserythropoietic anemia type III (CDA III). RNAi knockdown and rescue experiments in HeLa cells demonstrated that the p.P916R mutation causes cytokinesis failure, consistent with the large multinucleated erythroblasts observed in CDA III patients. Next-generation resequencing, haplotype analysis, RNAi knockdown and rescue in HeLa cells, functional cytokinesis assay Blood High 23570799
1998 CHO1/MKLP1 is associated with microtubules in podocytes and is required for establishing nonuniform (mixed) microtubule polarity. Antisense oligonucleotide-mediated depletion of CHO1/MKLP1 in differentiating podocytes abolished process formation and the nonuniform polarity of microtubules, demonstrating that MKLP1-dependent microtubule polarity is necessary for process formation. Antisense oligonucleotide treatment, hook-decoration of microtubules (polarity assay), taxol/nocodazole recovery experiments, immunolocalization The Journal of cell biology Medium 9864367
1998 CHO1/MKLP1 is expressed in postmitotic neurons including hippocampal, cortical, and cerebellar neurons. Expression peaks prior to dendritic development and decreases afterward. Dorsal root ganglion neurons that form axons but not dendrites express significantly lower levels, consistent with a role for MKLP1 in establishing the nonuniform microtubule polarity characteristic of dendrites. In situ hybridization (rodent brain sections), mRNA expression analysis in cultured neurons The European journal of neuroscience Low 9749792
2004 ZEN-4/MKLP1 functions postmitotically to establish foregut epithelium polarity in C. elegans. zen-4 mutants express polarity markers but fail to target PAR-3/Bazooka, PKC-3/aPKC, HMR-1/cadherin, and AJM-1 to correct cortical domains, and show disorganized microtubules and actin, indicating MKLP1 regulates an early step in epithelial polarization. Genetic mutant analysis, immunofluorescence of polarity markers, confocal microscopy in C. elegans Current biology : CB Medium 15182666
2006 Two nuclear localization signals (NLSs) in the tail domain of Mklp-1 were mapped to residues 899SRKRRSST906 and 949KRKKP953. Ectopic expression of an NLS-deleted mutant causes cell cycle arrest at cytokinesis. Phosphomimetic mutation of two serine residues in the first NLS (S→D) attenuates nuclear localization, suggesting NLS activity is regulated by phosphorylation. Deletion/mutation mapping, ectopic expression, fluorescence microscopy, cell cycle analysis Biochemical and biophysical research communications Medium 17198681
1999 The C-terminal domain of MKLP-1 contains at least one nuclear localization sequence; C-terminal constructs fused to GFP localize to the nucleus in HeLa cells and remain tightly associated with the nucleus following detergent and salt extraction, indicating stable interaction with a nuclear component. GFP-fusion expression in HeLa cells, subcellular fractionation, fluorescence microscopy Biochemical and biophysical research communications Low 10403813
2006 The motor domain and tail domain of MKLP1 are both required for dendritic targeting in hippocampal neurons. Deletion of the motor domain prevents dendritic distribution; deletion of the tail domain causes axonal mis-targeting; deletion of the stalk domain does not affect dendritic specificity. GFP-tagged domain deletion constructs expressed in primary hippocampal neurons, fluorescence microscopy Journal of cell science Medium 16418225
2013 p53 transcriptionally represses KIF23 mRNA and protein expression. This repression is mediated through the CDK inhibitor p21(WAF1/CIP1) and requires a cell cycle genes homology region (CHR) element in the KIF23 promoter. Cell cycle- and p53-dependent regulation of KIF23 involves differential binding of DREAM and MMB complexes to the CHR element. Promoter-reporter assays, chromatin immunoprecipitation (DREAM/MMB binding to CHR), siRNA knockdown of p21, RT-PCR and western blot PloS one Medium 23650552
2008 CUX1 and E2F1 cooperatively regulate KIF23/MKLP1 transcription at S-phase entry. Both transcription factors bind the MKLP1 promoter (demonstrated by ChIP) upon S-phase entry via CHR elements and E2F binding elements. Overexpression of either E2F1 or CUX1 increases endogenous MKLP1 levels; siRNA knockdown or dominant-negative E2F1 reduces expression. Chromatin immunoprecipitation, promoter-luciferase reporter assays, siRNA knockdown, overexpression Molecular and cellular biology Medium 19015243
2015 The two LATS/NDR kinase consensus sites (S716 and S814) in the long Kif23/CHO1 isoform are phosphorylated by NDR and LATS kinases in vitro. LATS1/2 contribute to S814 phosphorylation in vivo. Phosphorylation of the upstream S716 site is required for efficient phosphorylation at S814, revealing sequential phosphorylation. S814 phosphorylation constitutes a 14-3-3 binding site involved in Kif23 clustering during cytokinesis, and this modification is absent in post-abscission midbodies. In vitro kinase assay, site-directed mutagenesis, in vivo phosphorylation assay, 14-3-3 binding assay PloS one Medium 25658096
2016 In Drosophila motoneurons, the Toll-6 receptor–dSARM–FoxO signaling pathway represses expression of Pavarotti/MKLP1 (the Drosophila ortholog). Elevated Pavarotti/MKLP1 attenuates microtubule dynamics. Toll-6-FoxO signaling promotes MT dynamics by limiting Pav-KLP expression, and this pathway is essential for axon transport and activity-dependent structural plasticity in motoneurons. Genetic epistasis (Drosophila pathway mutants), genetic suppression, in vivo imaging of MT dynamics, overexpression of Pav-KLP The Journal of cell biology Medium 27502486
2013 v-Src induces cytokinesis failure by delocalizing Mklp1 from the spindle midzone. Aurora B, which regulates Mklp1 localization, and Mklp2 (responsible for Aurora B relocation from chromosomes to midzone) are also delocalized from the spindle midzone upon v-Src expression, placing Mklp1 downstream of the v-Src-Aurora B-Mklp2 axis. Inducible v-Src expression, flow cytometry, time-lapse microscopy, immunofluorescence localization Experimental cell research Medium 23562843
2016 p120-catenin (p120) binds directly to MKLP1 and to RhoA at the cleavage furrow during anaphase. The N-terminal coiled-coil domain of p120 isoform 1A is required for MKLP1 binding. p120 spatially controls RhoA GTPase cycling through concomitant binding to RhoA and MKLP1, and loss of p120 leads to multinucleation. Co-immunoprecipitation, immunofluorescence (cleavage furrow enrichment), domain deletion analysis, siRNA knockdown Nature communications Medium 28004812
2020 The tail domain of MKLP1 exhibits an autoinhibitory effect on its motor activity. Overexpression of the tail domain alone blocks cytokinesis and causes bi-/multinucleation. PAK2 (p21-activated kinase 2) binds to the MKLP1 tail domain (confirmed by GST pulldown, LC-MS/MS, co-IP, and FRET). PAK2 knockdown by siRNA mislocalizes MKLP1 from the midbody and impedes cytokinesis, suggesting PAK2 relieves MKLP1 autoinhibition to promote cytokinesis. GST pulldown followed by LC-MS/MS, co-immunoprecipitation, FRET, siRNA knockdown, overexpression BioMed research international Low 33204722 38550036
2022 The centralspindlin complex (Cyk4/Mklp1) and its interacting partner RhoGEF Ect2 are required for exclusion of NuMA/dynein/dynactin from the equatorial cell membrane during anaphase. The equatorial membrane enrichment of the Ect2/Cyk4/Mklp1 complex is essential for NuMA/dynein/dynactin exclusion and proper spindle elongation, linking centralspindlin to coordination of spindle elongation with cleavage furrow formation. siRNA depletion, live-cell imaging (membrane compartmentalization), immunofluorescence The Journal of cell biology Medium 36197340
2020 KIF23 directly interacts with APC membrane recruitment 1 (Amer1) protein. This interaction displaces Amer1 from the membrane/cytoplasm to the nucleus and blocks Amer1's association with APC, thereby attenuating Amer1's negative regulation of Wnt/β-catenin signaling and promoting nuclear β-catenin accumulation. Co-immunoprecipitation, immunofluorescence (subcellular localization of Amer1), functional rescue experiments, western blot Aging Medium 32365332
2024 SIRT7 interacts with KIF23 and inhibits succinylation of KIF23 at lysine K537. SIRT7-mediated desuccinylation enhances the protein stability of KIF23, and SIRT7 overexpression promotes anaplastic thyroid cancer cell viability and migration partly via KIF23. Co-immunoprecipitation, western blot for succinylation, overexpression/silencing in HEK-293T and ATC cells, cell viability/migration assays BMC cancer Medium 38360598
2023 FOXM1 epigenetically activates KIF23 expression by increasing RNA polymerase II and histone H3K27 acetylation at the KIF23 promoter. Downregulation of FOXM1 reduces KIF23 levels and alleviates sorafenib resistance in hepatocellular carcinoma cells. ChIP for RNA pol II and H3K27ac at KIF23 promoter, siRNA knockdown, western blot, drug resistance assays Biochemical and biophysical research communications Medium 36940637
2024 KIF23 knockdown in embryonic mouse cortex causes precocious neurogenesis and neuronal apoptosis, attributed to accelerated cell cycle exit from disrupted mitotic spindle orientation and impaired cytokinesis. KIF23 depletion also perturbs apical surface structure of neural stem and progenitor cells by affecting localization of apical junction proteins. Wild-type human KIF23 rescues these phenotypes, but a microcephaly-associated KIF23 variant does not. In utero electroporation knockdown in mice, immunofluorescence, live imaging, rescue with wild-type vs. variant human KIF23 The EMBO journal High 39632980
2002 Zebrafish Mklp1 redistributes from spindle microtubules at metaphase to the spindle midzone during anaphase and concentrates in the midbody during telophase/cytokinesis. Nuclear targeting is conferred by two basic motifs in the COOH terminus. Dominant-negative Mklp1 variants in one- or two-cell zebrafish embryos cause failure to complete cytokinesis, resulting in multinucleated blastomeres. GFP-tagging and live imaging in zebrafish embryos, dominant-negative mRNA injection, fractionation Physiological genomics Medium 11842131
2022 NAT10-mediated ac4C modification of KIF23 mRNA at its 3'UTR region stabilizes KIF23 mRNA and elevates KIF23 protein levels, which in turn activates the Wnt/β-catenin pathway to promote colorectal cancer progression. GSK-3β negatively regulates NAT10 forming a feedback loop. RIP-seq, acRIP-seq, RNA immunoprecipitation, luciferase reporter assay, siRNA knockdown, xenograft models Journal of experimental & clinical cancer research : CR Medium 36522719
2019 Neddylation inhibition by MLN4924 causes premature accumulation of MKLP1 at the cleavage furrow during mitosis, which is associated with increased abscission delay and failure. NEDD8 and CSN subunits localize to the midbody and cleavage furrow, indicating neddylation/deneddylation regulation at the midbody controls MKLP1 timing. MLN4924 drug treatment in synchronized cells, fixed and live-cell microscopy, immunofluorescence Cell cycle (Georgetown, Tex.) Low 31057046
2026 The C-terminal domain of KIF23 directly binds to the myosin tail domain of MYH9. This interaction stabilizes MYH9 by recruiting deubiquitinase USP7, which removes K48-linked ubiquitin chains from MYH9. Elevated MYH9 then promotes recruitment of USP15 to deubiquitinate MCM2 at K469, preventing MCM2 degradation and enabling MCM2-PCNA interaction to promote cell cycle progression and cisplatin resistance in cervical cancer. Co-immunoprecipitation, protein half-life assays, ubiquitination assays, CRISPR/Cas9 knockout, site-directed mutagenesis Clinical and translational medicine Medium 41940421
2025 In cardiac fibroblasts, Kif23 promotes fibrosis by activating the RhoA/ROCK1 signaling axis, which suppresses Ces1d-mediated fatty acid β-oxidation, leading to lipid accumulation and myofibroblast transdifferentiation. Kif23 knockdown in vivo (post-MI mouse model) improved cardiac function and attenuated fibrosis. Proteomic profiling of knockdown vs. overexpression fibroblasts, adeno-associated virus shRNA knockdown in vivo, western blot, lipid droplet analysis, echocardiography Hypertension Medium 41078122
2025 ETV5 transcription factor directly binds to the KIF23 promoter region (within 1–700 bp upstream of the transcription start site) and represses KIF23 transcription. ETV5 overexpression suppresses caspase-3/GSDME-mediated pyroptosis and cognitive impairment in a perioperative neurocognitive disorder model, and this protective effect is neutralized by KIF23 overexpression. Chromatin immunoprecipitation (ETV5 binding to KIF23 promoter), AAV9-mediated overexpression/knockdown in mice, western blot, cognitive behavior assays Biochemical pharmacology Medium 40983152
2021 SHCBP1 interacts with KIF23 via its Nesd homology domain (NHD) and this interaction is important for KIF23's nuclear localization. SHCBP1 positively modulates KIF23 expression. KIF23 knockdown abrogates cisplatin resistance induced by SHCBP1 overexpression, placing KIF23 downstream of SHCBP1 in modulating cisplatin resistance. Co-immunoprecipitation (domain mapping), siRNA knockdown, overexpression, drug sensitivity assays Head & neck Low 34918847
2025 CDK1 and Aurora B phosphorylate two serine residues (S440 and S699) on Citron kinase (CIT-K), and phosphorylation at either residue reduces the ability of CIT-K to interact with its midbody partners including KIF23/MKLP1, thereby regulating midbody formation and stability. In vitro kinase assay, phospho-mutant analysis, co-immunoprecipitation, live-cell imaging bioRxivpreprint Low

Source papers

Stage 0 corpus · 81 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1995 Plk is an M-phase-specific protein kinase and interacts with a kinesin-like protein, CHO1/MKLP-1. Molecular and cellular biology 246 8524282
2005 Phosphorylation of ZEN-4/MKLP1 by aurora B regulates completion of cytokinesis. Current biology : CB 179 15854913
2022 Acetyltransferase NAT10 regulates the Wnt/β-catenin signaling pathway to promote colorectal cancer progression via ac4C acetylation of KIF23 mRNA. Journal of experimental & clinical cancer research : CR 134 36522719
2007 Cooperative assembly of CYK-4/MgcRacGAP and ZEN-4/MKLP1 to form the centralspindlin complex. Molecular biology of the cell 95 17942600
2005 Recruitment of MKLP1 to the spindle midzone/midbody by INCENP is essential for midbody formation and completion of cytokinesis in human cells. The Biochemical journal 82 15796717
2011 Downregulation of KIF23 suppresses glioma proliferation. Journal of neuro-oncology 80 21904957
2015 Overexpression of KIF23 predicts clinical outcome in primary lung cancer patients. Lung cancer (Amsterdam, Netherlands) 79 26775597
2004 Molecular interactions of Polo-like-kinase 1 with the mitotic kinesin-like protein CHO1/MKLP-1. Journal of cell science 78 15199097
2013 Congenital dyserythropoietic anemia type III (CDA III) is caused by a mutation in kinesin family member, KIF23. Blood 74 23570799
2023 A Therapeutically Targetable TAZ-TEAD2 Pathway Drives the Growth of Hepatocellular Carcinoma via ANLN and KIF23. Gastroenterology 62 36894036
1999 Arf proteins bind to mitotic kinesin-like protein 1 (MKLP1) in a GTP-dependent fashion. Cell motility and the cytoskeleton 58 10506747
2012 Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. The EMBO journal 51 22522702
2013 TRAF6 mediates ubiquitination of KIF23/MKLP1 and is required for midbody ring degradation by selective autophagy. Autophagy 48 24128730
2019 KIF23 Promotes Gastric Cancer by Stimulating Cell Proliferation. Disease markers 46 31007778
2013 p53 and cell cycle dependent transcription of kinesin family member 23 (KIF23) is controlled via a CHR promoter element bound by DREAM and MMB complexes. PloS one 45 23650552
2004 ZEN-4/MKLP1 is required to polarize the foregut epithelium. Current biology : CB 45 15182666
1998 Nonuniform microtubular polarity established by CHO1/MKLP1 motor protein is necessary for process formation of podocytes. The Journal of cell biology 45 9864367
2008 CUX1 and E2F1 regulate coordinated expression of the mitotic complex genes Ect2, MgcRacGAP, and MKLP1 in S phase. Molecular and cellular biology 43 19015243
2016 An important role for Myb-MuvB and its target gene KIF23 in a mouse model of lung adenocarcinoma. Oncogene 39 27212033
2019 Methylation-mediated repression of MiR-424/503 cluster promotes proliferation and migration of ovarian cancer cells through targeting the hub gene KIF23. Cell cycle (Georgetown, Tex.) 38 31135262
2020 KIF23 enhances cell proliferation in pancreatic ductal adenocarcinoma and is a potent therapeutic target. Annals of translational medicine 37 33313139
2016 A Toll receptor-FoxO pathway represses Pavarotti/MKLP1 to promote microtubule dynamics in motoneurons. The Journal of cell biology 37 27502486
2013 v-Src causes delocalization of Mklp1, Aurora B, and INCENP from the spindle midzone during cytokinesis failure. Experimental cell research 37 23562843
2020 KIF23 activated Wnt/β-catenin signaling pathway through direct interaction with Amer1 in gastric cancer. Aging 36 32365332
1998 Expression of the mitotic motor protein CHO1/MKLP1 in postmitotic neurons. The European journal of neuroscience 36 9749792
2020 Knockdown of lncRNA PVT1 inhibits prostate cancer progression in vitro and in vivo by the suppression of KIF23 through stimulating miR-15a-5p. Cancer cell international 31 32624708
2021 KIF23 promotes triple negative breast cancer through activating epithelial-mesenchymal transition. Gland surgery 30 34268078
2016 Kinesin family members KIF11 and KIF23 as potential therapeutic targets in malignant pleural mesothelioma. International journal of oncology 30 27279560
2002 Zebrafish mitotic kinesin-like protein 1 (Mklp1) functions in embryonic cytokinesis. Physiological genomics 29 11842131
2006 The nuclear localization signal of mitotic kinesin-like protein Mklp-1: effect on Mklp-1 function during cytokinesis. Biochemical and biophysical research communications 26 17198681
2021 Aberrant KIF23 expression is associated with adverse clinical outcome and promotes cellular malignant behavior through the Wnt/β-catenin signaling pathway in Colorectal Cancer. Journal of Cancer 23 33754001
2016 p120-catenin prevents multinucleation through control of MKLP1-dependent RhoA activity during cytokinesis. Nature communications 23 28004812
2024 SIRT7 promotes the proliferation and migration of anaplastic thyroid cancer cells by regulating the desuccinylation of KIF23. BMC cancer 21 38360598
2017 Mutation analysis and copy number alterations of KIF23 in non-small-cell lung cancer exhibiting KIF23 over-expression. OncoTargets and therapy 18 29066916
2021 Functional roles of the SHCBP1 and KIF23 interaction in modulating the cell-cycle and cisplatin resistance of head and neck squamous cell carcinoma. Head & neck 16 34918847
2023 KIF23, under regulation by androgen receptor, contributes to nasopharyngeal carcinoma deterioration by activating the Wnt/β-catenin signaling pathway. Functional & integrative genomics 15 37010644
2006 MKLP1 requires specific domains for its dendritic targeting. Journal of cell science 15 16418225
2021 MiR-17-5p downregulation alleviates apoptosis and fibrosis in high glucose-induced human mesangial cells through inactivation of Wnt/β-catenin signaling by targeting KIF23. Environmental toxicology 14 34014023
2024 KIF23 promotes cervical cancer progression via inhibiting NLRP3-mediated pyroptosis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 13 38780518
2017 Temporal regulation of epithelium formation mediated by FoxA, MKLP1, MgcRacGAP, and PAR-6. Molecular biology of the cell 13 28539408
2015 Binding of Kif23-iso1/CHO1 to 14-3-3 is regulated by sequential phosphorylations at two LATS kinase consensus sites. PloS one 13 25658096
2022 Knockdown of circ_0067934 inhibits gastric cancer cell proliferation, migration and invasion via the miR‑1301‑3p/KIF23 axis. Molecular medicine reports 12 35475447
2022 KIF23 promotes autophagy-induced imatinib resistance in chronic myeloid leukaemia through activating Wnt/β-catenin pathway. Clinical and experimental pharmacology & physiology 12 36066385
2020 Kinesin family member 23 (KIF23) contributes to the progression of bladder cancer cells in vitro and in vivo. Neoplasma 12 33231086
2008 Zygotic loss of ZEN-4/MKLP1 results in disruption of epidermal morphogenesis in the C. elegans embryo. Developmental dynamics : an official publication of the American Association of Anatomists 12 18265015
2022 Inhibition of KIF23 Alleviates IPAH by Targeting Pyroptosis and Proliferation of PASMCs. International journal of molecular sciences 11 35457254
1999 Nuclear localization of C-terminal domains of the kinesin-like protein MKLP-1. Biochemical and biophysical research communications 11 10403813
2023 FOXM1 augments sorafenib resistance and promotes progression of hepatocellular carcinoma by epigenetically activating KIF23 expression. Biochemical and biophysical research communications 10 36940637
2021 Systematic Pan-Cancer Analysis of KIF23 and a Prediction Model Based on KIF23 in Clear Cell Renal Cell Carcinoma (ccRCC). Pharmacogenomics and personalized medicine 10 35002290
2021 Mutation and Copy Number Alterations Analysis of KIF23 in Glioma. Frontiers in genetics 9 34017355
2024 Mechanism of regulation of KIF23 on endometrial cancer cell growth and apoptosis. Discover oncology 8 38514510
2024 Kinesin-like motor protein KIF23 maintains neural stem and progenitor cell pools in the developing cortex. The EMBO journal 8 39632980
2022 KIF23 is a potential biomarker of diffuse large B cell lymphoma: Analysis based on bioinformatics and immunohistochemistry. Medicine 8 35713434
2020 Congenital dyserythropoietic anemia types Ib, II, and III: novel variants in the CDIN1 gene and functional study of a novel variant in the KIF23 gene. Annals of hematology 8 33159567
2024 Identification and validation of KIF23 as a hypoxia-regulated lactate metabolism-related oncogene in uterine corpus endometrial carcinoma. Life sciences 7 38336274
2024 Regulation of KIF23 by miR-107 controls replicative tumor cell fitness in mouse and human hepatocellular carcinoma. Journal of hepatology 7 40235270
2022 DEP domain containing 1B (DEPDC1B) exerts the tumor promoter in hepatocellular carcinoma through activating p53 signaling pathway via kinesin family member 23 (KIF23). Bioengineered 7 34983303
2024 Knockdown of KIF23 alleviates the progression of asthma by inhibiting pyroptosis. BMJ open respiratory research 6 38569671
2022 Membrane compartmentalization of Ect2/Cyk4/Mklp1 and NuMA/dynein regulates cleavage furrow formation. The Journal of cell biology 6 36197340
2025 KIF23 inhibition protects against perioperative neurocognitive disorders by hindering ROS/caspase-3/GSDME-mediated pyroptosis. Experimental neurology 5 40639436
2020 Differential tissue specific expression of Kif23 alternative transcripts in mice with the human mutation causing congenital dyserythropoietic anemia type III. Blood cells, molecules & diseases 5 32818800
2020 Revealing PAK2's Function in the Cell Division through MKLP1's Interactome. BioMed research international 5 33204722
2023 KCNQ1OT1 promotes retinoblastoma progression by targeting miR-339-3p that suppresses KIF23. International ophthalmology 3 37198502
2019 Inhibition of neddylation induces mitotic defects and alters MKLP1 accumulation at the midbody during cytokinesis. Cell cycle (Georgetown, Tex.) 3 31057046
1998 Sequence and expression of DmMKLP1, a homolog of the human MKLP1 kinesin-like protein from Drosophila melanogaster. Development genes and evolution 3 9799428
2025 Cancer-associated fibroblast-derived exosomal FAM83F regulates KIF23 expression to promote the malignant progression and reduce radiosensitivity in non-small cell lung cancer. Cytotechnology 2 39867833
2025 Kif23 Promotes Myocardial Fibrosis by Suppressing Ces1d-Dependent Lipid Metabolism. Hypertension (Dallas, Tex. : 1979) 2 41078122
2025 Assessment of anti-cancer activity of cyclovirobuxine D in nasopharyngeal carcinoma cells: Involvement of KIF23-mediated Akt/mTOR pathway. Pathology, research and practice 1 40902449
2025 ETV5 transcriptionally inhibits KIF23 to repress pyroptosis in aged mice with perioperative neurocognitive disorders. Biochemical pharmacology 1 40983152
2024 Molecular structure of polysaccharide mediated autophagy markers KIF23 and PRC1 proteins and their regulatory role in triple negative cancer through the p53 signaling pathway. International journal of biological macromolecules 1 39725112
2026 KIF23 Overexpression Promotes Cell Viability, Migration, and Invasion via the Wnt/β-Catenin Signaling Pathway in Anaplastic Thyroid Carcinoma. International journal of endocrinology 0 41675939
2026 NCBP2 Regulates PGAM5-Mediated Mitophagy Via KIF23 Alternative Splicing To Promote Cervical Cancer ProgressionRun Title: NCBP2 Promotes Cervical Cancer Via Mitophagy. Applied biochemistry and biotechnology 0 41874817
2026 Targeting KIF23 inhibits cell proliferation and primary chemoresistance in cervical cancer by inactivating the MYH9/MCM2/PCNA pathway. Clinical and translational medicine 0 41940421
2026 DLX6-AS1 promotes the progression of Wilms tumor by sponging miR-195-5p to upregulate KIF23 in Wilms tumor cells. Discover oncology 0 42154117
2026 KIF23 in disease pathogenesis and its therapeutic and diagnostic potential. Discover oncology 0 42178466
2025 RETRACTION: KIF23 Promotes Gastric Cancer by Stimulating Cell Proliferation. Disease markers 0 40726601
2025 Congenital Dyserythropoietic Anemia Type III Associated With a Novel KIF23 Variant (c.2132A>G; p.Gln711Arg): A Case Report. Clinical case reports 0 40927401
2025 KIF23 silencing suppresses papillary thyroid carcinoma metastasis by regulating mitophagy via Wnt/β-catenin pathway. Endocrine connections 0 41138746
2025 A core stemness-associated module reveals PLK1, NUF2, KIF23, CDCA8, TOP2A, CENPF, AURKA, and ASPM as key genes in rectal cancer. European journal of medical research 0 41373021
2024 Retracted: Revealing PAK2's Function in the Cell Division through MKLP1's Interactome. BioMed research international 0 38550036
2024 [Corrigendum] Knockdown of circ_0067934 inhibits gastric cancer cell proliferation, migration and invasion via the miR‑1301‑3p/KIF23 axis. Molecular medicine reports 0 39155879

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