Affinage

MAP1B

Microtubule-associated protein 1B · UniProt P46821

Length
2468 aa
Mass
270.6 kDa
Annotated
2026-06-10
100 papers in source corpus 43 papers cited in narrative 43 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

MAP1B is a neuronally enriched microtubule-associated phosphoprotein that integrates microtubule and actin cytoskeletal dynamics to drive axon growth, guidance, neuronal migration, and synaptic plasticity (PMID:11121433, PMID:10973990, PMID:20719958). It is synthesized as a polyprotein yielding a heavy chain and light chain 1 (LC1) by proteolytic processing, with the heavy chain binding microtubules through a basic KKEE/KKEVI-repeat domain unrelated to those of MAP2 or tau (PMID:2480963, PMID:1712602); LC1 independently stabilizes microtubules, promotes tubulin polymerization, and carries a C-terminal actin-binding domain (PMID:9813091, PMID:11896150), and the heavy chain itself directly binds actin, making MAP1B a bivalent crosslinker of both cytoskeletal systems (PMID:17292804). Beyond stabilizing and acetylating microtubules and enhancing their assembly rate (PMID:1487506, PMID:22033417), MAP1B sequesters the +TIP proteins EB1/EB3 in the cytosol to restrain plus-end dynamics and prevent microtubule overstabilization in growth cones (PMID:23572079). MAP1B activity is governed by a phosphorylation switch: GSK-3β phosphorylates it at Ser1260/Thr1265 (and is primed by DYRK1A) to toggle microtubule dynamic instability selectively in growing axons, with CK2, NLK, and a DLK–MKK7–JNK1 module as additional kinases and PP2A/PP2B/PP1 reversing these modifications to restore microtubule binding (PMID:3164313, PMID:15731007, PMID:19549690, PMID:7690334, PMID:19840224, PMID:23226105); this GSK-3β input is itself relayed from TrkA–MAPK, Wnt, Netrin-1, and Reelin signaling for axon growth and migration (PMID:9570753, PMID:15186740, PMID:15737742, PMID:14622124). Through interaction with the Rac1 GEF Tiam1, MAP1B controls a Rac1/Cdc42/RhoA balance required for axon outgrowth, dendritic spine maturation, and LTD-associated AMPA receptor endocytosis (PMID:20719958, PMID:21984824, PMID:23881099). MAP1B additionally anchors GABA(C) receptors at retinal synapses via the rho1 subunit to tune receptor sensitivity (PMID:9892354, PMID:11102469) and facilitates Nav1.6 surface trafficking (PMID:22474336). Its LC1 is targeted for ubiquitin-dependent degradation by gigaxonin, whose loss causes MAP1B-LC accumulation and neuronal death in giant axonal neuropathy (PMID:16227972), and MAP1B mutations that reduce protein levels and phosphorylation cause hereditary hearing loss with disturbed microtubule dynamics in sensory neurons (PMID:33268592).

Mechanistic history

Synthesis pass · year-by-year structured walk · 39 steps
  1. 1989 High

    Established the structural basis of MAP1B's microtubule binding, defining a novel basic repeat domain distinct from other MAPs.

    Evidence Cell-free translation of subcloned fragments with in vitro microtubule cosedimentation plus transfected-cell deletion analysis

    PMID:2480963

    Open questions at the time
    • No atomic structure of the binding interface
    • Affinity and stoichiometry of binding not quantified
  2. 1991 High

    Resolved how MAP1B generates its multi-subunit architecture by showing heavy chain and LC1 arise from one polyprotein.

    Evidence Protein sequencing, epitope mapping, and Northern/Southern blotting with heavy chain–LC1 co-purification

    PMID:1712602

    Open questions at the time
    • Protease responsible for processing not identified
    • Regulation of processing not addressed
  3. 1988 High

    Identified CK2 as a principal kinase acting on MAP1B during neurite outgrowth, opening the phosphoregulation question.

    Evidence In vitro kinase assay with purified CK2 plus phosphopeptide mapping against in vivo neuroblastoma MAP1B

    PMID:3164313

    Open questions at the time
    • Specific CK2 sites not mapped
    • Functional consequence of CK2 phosphorylation not defined here
  4. 1992 High

    Demonstrated MAP1B is a functional microtubule stabilizer in cells, distinguishing it from the bundling MAPs.

    Evidence Heterologous cDNA transfection with nocodazole resistance and acetylated-tubulin readouts across multiple cell lines

    PMID:1487506

    Open questions at the time
    • Does not separate heavy-chain vs light-chain contributions
    • Mechanism of acetylation increase unresolved
  5. 1998 High

    Dissected the light chain as an autonomous stabilizing/actin-binding module under heavy-chain restraint.

    Evidence COS-cell transfection, drug-resistance assays, actin cosedimentation, and co-IP domain mapping of tagged fragments

    PMID:9813091

    Open questions at the time
    • In vivo relevance of LC1 oligomerization unclear
    • Heavy-chain inhibition mechanism not structurally defined
  6. 1994 High

    Showed a shared light chain (LC3) confers direct microtubule binding to both MAP1A and MAP1B complexes.

    Evidence cDNA sequencing with recombinant LC3 microtubule cosedimentation and immunoprecipitation

    PMID:7908909

    Open questions at the time
    • Functional distinction of LC3 vs LC1 within MAP1B not addressed
    • Stoichiometry within the complex unknown
  7. 1999 High

    Connected MAP1B to receptor anchoring by identifying a specific GABA(C) rho1 interaction at retinal synapses.

    Evidence Yeast two-hybrid, co-IP, retinal co-localization, and COS redistribution assay

    PMID:9892354

    Open questions at the time
    • Did not yet show functional effect on receptor activity
  8. 2000 High

    Showed cytoskeletal anchoring by MAP1B tunes GABA(C) receptor sensitivity, giving the interaction physiological meaning.

    Evidence rho1 domain-mapping mutagenesis plus patch-clamp of retinal bipolar cells after interaction disruption

    PMID:11102469

    Open questions at the time
    • Mechanism linking anchoring to EC50 shift unresolved
  9. 2000 High

    Defined MAP1B's essential developmental roles via complete-null mice and revealed redundancy with tau.

    Evidence Gene targeting with histology and nerve conduction measurement; tau/map1b double-knockout epistasis in cultured neurons

    PMID:10973990 PMID:11121433

    Open questions at the time
    • Molecular basis of corpus callosum-selective requirement unclear
    • Extent of redundancy with other MAPs beyond tau not tested
  10. 2005 High

    Pinpointed GSK-3β phosphosites (Ser1260/Thr1265) as a spatial molecular switch for microtubule dynamics in growing axons.

    Evidence Site-directed mutagenesis, in vitro GSK-3β kinase assay, phospho-specific antibodies, in vivo localization, and MT dynamics in transfected cells

    PMID:15731007

    Open questions at the time
    • How phosphorylation alters MT-binding biophysically not resolved
    • Interplay with other phosphosites not addressed
  11. 2004 Medium

    Placed MAP1B as a downstream effector of Netrin-1 and Reelin axon-guidance/migration signaling via mode I phosphorylation.

    Evidence In vitro phosphorylation assays, mutant-mouse anatomy/phenocopy, and chemoattraction/lamination analyses

    PMID:15186740 PMID:15590913

    Open questions at the time
    • Direct linkage of GSK-3/CDK5 activation to specific MAP1B sites not mapped
    • mDab1 role only partially defined
  12. 2005 Medium

    Traced the upstream growth-factor route to MAP1B by showing NGF–TrkA–MAPK (not PI3K or p75) drives GSK-3β phosphorylation of MAP1B.

    Evidence Receptor-selective ligands, TrkA-null PC12 cells, K252a inhibition, and pharmacological MAPK/PI3K dissection

    PMID:14622124 PMID:15737742

    Open questions at the time
    • Relies on inhibitor specificity
    • Intermediate steps between MAPK and GSK-3β not defined
  13. 2005 High

    Identified gigaxonin-controlled proteasomal degradation of MAP1B-LC as a determinant of neuronal survival, linking MAP1B to giant axonal neuropathy.

    Evidence Pull-down/co-IP, proteasome inhibition, GAN-null mouse neurons, and MAP1B siRNA rescue of survival

    PMID:16227972

    Open questions at the time
    • Mechanism by which MAP1B-LC excess kills neurons not defined
    • Ubiquitination sites on MAP1B-LC not mapped
  14. 2009 High

    Expanded the phosphocode by establishing DYRK1A as a priming kinase generating distinct spatial pools of GSK-3β-phosphorylated MAP1B.

    Evidence Mass spectrometry mapping of 28 sites, phospho-antibody panel, DYRK1A shRNA, and EB3 MT-dynamics imaging

    PMID:19549690

    Open questions at the time
    • Functional consequence of each phosphosite class not individually tested
  15. 2009 Medium

    Added NLK as a direct MAP1B kinase coupling NGF signaling to actin redistribution and neurite outgrowth.

    Evidence In vitro kinase assay with purified NLK plus NLK knockdown with F-actin and outgrowth readouts

    PMID:19840224

    Open questions at the time
    • NLK target sites on MAP1B not mapped
    • Single lab
  16. 1993 Medium

    Defined the phosphatase arm of the switch, distinguishing PP2A/PP2B/PP1 specificities for mode I vs mode II sites.

    Evidence In vitro phosphatase assays with purified enzymes plus okadaic acid/cyclosporin A treatment of brain slices with MT-binding readout

    PMID:7690334

    Open questions at the time
    • In vivo phosphatase targeting/regulation not addressed
  17. 2000 Medium

    Established PP2A as the dominant phosphatase controlling MAP1B microtubule-binding activity in brain.

    Evidence Okadaic acid/cyclosporin A treatment of metabolically active brain slices with phospho-MAP1B and MT-binding assays

    PMID:10640627

    Open questions at the time
    • Relies on inhibitor specificity
    • PP2A holoenzyme composition not defined
  18. 1996 Medium

    Showed phosphorylation negatively regulates MAP1B–F-actin binding, linking the phosphoswitch to the actin arm.

    Evidence In vitro alkaline-phosphatase dephosphorylation with F-actin cosedimentation

    PMID:8690071

    Open questions at the time
    • Single in vitro method
    • Specific PDPK site responsible not pinpointed
  19. 2007 Medium

    Confirmed the heavy chain is itself a bivalent actin/microtubule binder, establishing MAP1B as a direct cytoskeletal crosslinker.

    Evidence Brain co-IP, mass spectrometry, atomic force microscopy force measurement, and electron microscopy

    PMID:17292804

    Open questions at the time
    • Actin-binding site on heavy chain not mapped
    • Single lab
  20. 2007 Medium

    Demonstrated MAP1B is required for coordinated microtubule–actin remodeling during process retraction and Schwann cell migration.

    Evidence Map1b-null neurons and Schwann cells with LPA stimulation, live cytoskeletal imaging, and migration assays

    PMID:17764972

    Open questions at the time
    • Molecular step coupling actin contraction to MT backfolding undefined
  21. 2002 Medium

    Refined LC1 domain architecture, separating its N-terminal MT-polymerizing and C-terminal actin-binding activities.

    Evidence Transfected-cell binding assays, in vitro tubulin polymerization, and domain deletion analysis

    PMID:11896150

    Open questions at the time
    • Structural basis of LC1 vs LC2 functional divergence unresolved
  22. 2008 Medium

    Linked MAP1B to autophagy and cell death by identifying it as a DAPK-1 binding partner required for DAPK-1-driven phenotypes.

    Evidence Peptide-library binding, starvation-induced co-IP, siRNA loss-of-function, and autophagy/blebbing/clonogenic assays

    PMID:18195017

    Open questions at the time
    • Whether MAP1B is a DAPK-1 substrate not established
    • Single lab
  23. 2010 High

    Defined the MAP1B–Tiam1–Rac1 axis coordinating actin remodeling and microtubule–actin crosstalk for neuronal polarization.

    Evidence MAP1B-null neuron GTPase activity assays, Tiam1 co-IP, and rescue with constitutively active Rac1/Cdc42/Tiam1

    PMID:20719958

    Open questions at the time
    • Direct vs indirect MAP1B–Tiam1 binding interface not mapped
  24. 2011 High

    Extended MAP1B function to dendritic spine maturation through Rac1/RhoA/cofilin actin regulation.

    Evidence MAP1B+/- neurons with spine morphology, patch-clamp, GTPase assays, and PSD Western blots

    PMID:21984824

    Open questions at the time
    • How MAP1B controls cofilin phosphorylation not mechanistically resolved
  25. 2011 High

    Quantified MAP1B's contribution to microtubule assembly rate along the axon shaft.

    Evidence RNAi and gain-of-function with EB3-GFP polymerization-speed imaging and axon morphology

    PMID:22033417

    Open questions at the time
    • Molecular mechanism of growth-rate enhancement not defined
  26. 2011 Medium

    Established post-transcriptional control of Map1b via Stau2-dependent dendritic mRNA localization required for mGluR-LTD.

    Evidence Stau2 shRNA, Map1b mRNA FISH, dendritic protein quantification, and LTP/LTD electrophysiology

    PMID:21508097

    Open questions at the time
    • Translational regulation step downstream of localization not detailed
  27. 2012 Medium

    Revealed MAP1B as a major Syk substrate promoting microtubule stability in non-neuronal cancer cells.

    Evidence Syk expression/silencing, MAP1B siRNA epistasis, drug-resistance/acetylation assays, and AFM nanomechanics

    PMID:24914616

    Open questions at the time
    • Syk phosphosites on MAP1B not mapped
    • Single lab
  28. 2012 High

    Identified MAP1B-LC as a target of S-nitrosylation-dependent ubiquitination by MITOL linking it to mitochondrial integrity.

    Evidence S-nitrosylation and ubiquitination assays, MITOL manipulation, and mitochondrial-function/cell-death readouts

    PMID:22308378

    Open questions at the time
    • Nitrosylated cysteine and ubiquitination sites not mapped
    • Relationship to gigaxonin pathway unresolved
  29. 2012 High

    Showed MAP1B facilitates Nav1.6 surface trafficking, broadening its membrane-protein chaperoning role.

    Evidence Yeast two-hybrid, brain co-IP, alanine-scanning of the Nav1.6 N-terminus, and patch-clamp of transfected cells

    PMID:22474336

    Open questions at the time
    • Trafficking mechanism (vs stabilization) not distinguished
  30. 2012 High

    Connected MAP1B to centrosomal MT organization and Golgi/trafficking integrity via dystonin-a2.

    Evidence Dystonin mutant mice, isoform-specific RNAi, co-IP, and rescue by trichostatin A or MAP1B overexpression

    PMID:22412020

    Open questions at the time
    • Direct vs scaffolded MAP1B–dystonin contact not resolved
  31. 2012 Medium

    Added a locally translated DLK–MKK7–JNK1 module phosphorylating Map1b to control growth-cone microtubule bundling.

    Evidence Genome-wide mRNA localization screen, MKK7 FISH, phospho-Map1b staining, and kinase inhibitor/dominant-negative assays

    PMID:23226105

    Open questions at the time
    • JNK1 target sites on Map1b not mapped
  32. 2013 High

    Uncovered MAP1B cytosolic sequestration of EB1/EB3 as a brake on plus-end dynamics governing axon outgrowth.

    Evidence Co-IP, direct binding, RNAi, and EB3-GFP live imaging with growth-cone morphology

    PMID:23572079

    Open questions at the time
    • Binding interface and competition with MT plus-ends not structurally defined
  33. 2013 High

    Established a MAP1B–Tiam1–Rac1 relay required for AMPA receptor endocytosis during LTD.

    Evidence Conditional MAP1B deletion/shRNA, LTD electrophysiology, endocytosis and Tiam1 localization assays, and Rac1 rescue

    PMID:23881099

    Open questions at the time
    • How MAP1B targets Tiam1 to synapses mechanistically unclear
  34. 2016 Medium

    Showed local dephosphorylation of MAP1B at branch points, downstream of GSK-3, controls neurite branching and protects modified microtubules.

    Evidence Map1b-null neurons, GSK-3 inhibition, cDNA rescue, fibroblast nocodazole assays, and tyrosinated/acetylated tubulin staining

    PMID:26773468

    Open questions at the time
    • Phosphatase responsible for local dephosphorylation not identified
  35. 2016 Medium

    Identified a presynaptic structural/functional role for MAP1B in terminal and vesicle organization.

    Evidence MAP1B KO neurons with terminal-density immunofluorescence, FM4-64 fusion assay, and electron microscopy

    PMID:27425640

    Open questions at the time
    • Molecular partners mediating presynaptic role not defined
  36. 2006 Medium

    Defined QKI-dependent mRNA stabilization as oligodendroglial post-transcriptional control of MAP1B levels.

    Evidence 3'UTR binding, qv mutant mice, QKI knockdown/overexpression, and mRNA-stability measurements

    PMID:16855020

    Open questions at the time
    • Functional consequence of MAP1B in oligodendroglia not tested here
  37. 2015 Medium

    Revealed FMRP/miR-181d-mediated repression of axonal MAP1B local translation released by NGF.

    Evidence FMRP co-IP with miR-181d/Map1b mRNA, microfluidic axon isolation, axonal protein quantification, and Fmr1 mutant mice

    PMID:26711345

    Open questions at the time
    • Direct miR-181d targeting of Map1b 3'UTR not fully validated
    • Single lab
  38. 2019 Medium

    Linked reduced MAP1B expression to Parkinson's-related neuritic defects through Nrf2 transcriptional control at the Map1b enhancer.

    Evidence Isogenic SNCA-A53T iPSC dopaminergic neurons, Nrf2 ChIP/reporter assays, and MAP1B/Nrf2 rescue of morphology

    PMID:31235589

    Open questions at the time
    • Causal contribution of MAP1B loss to PD pathology in vivo not established
  39. 2020 High

    Demonstrated MAP1B mutations cause hereditary hearing loss via reduced levels/phosphorylation and disturbed microtubule dynamics in sensory neurons.

    Evidence Patient iPSC-derived otic neurons with CRISPR correction, Map1b+/- mouse audiometry, and MT-dynamics/electrophysiology readouts

    PMID:33268592

    Open questions at the time
    • How specific missense changes impair phosphorylation not mechanistically resolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the many converging kinase inputs, phosphosites, and binding partners are spatially integrated into a single dynamic regulation of MAP1B at defined subcellular sites remains unresolved.
  • No structural model of full-length MAP1B or its phosphorylated states
  • Site-by-site functional decoding of the 28+ phosphosites incomplete
  • Quantitative integration of competing actin/MT/+TIP/receptor interactions undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 7 GO:0060090 molecular adaptor activity 4 GO:0098772 molecular function regulator activity 3
Localization
GO:0005856 cytoskeleton 3 GO:0005815 microtubule organizing center 1 GO:0005829 cytosol 1
Pathway
R-HSA-112316 Neuronal System 6 R-HSA-1266738 Developmental Biology 5 R-HSA-162582 Signal Transduction 5
Partners
DAPK1DSTEB1EB3GANMARCHF5SCN8ATIAM1
Complex memberships
MAP1B heavy chain–light chain (LC1/LC3) complex

Evidence

Reading pass · 43 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1989 The microtubule-binding domain of MAP1B was mapped to a highly basic region containing repeated KKEE/KKEVI motifs; this region copurifies with microtubules in vitro and mediates microtubule interaction in vivo in transfected cells. A separate 12-repeat region does not bind microtubules. The MAP1B microtubule-binding domain is structurally unrelated to those of MAP2, tau, or kinesin. Cell-free translation of subcloned fragments + microtubule cosedimentation cycles in vitro; full-length and deletion constructs transfected into cultured cells with immunofluorescence readout The Journal of Cell Biology High 2480963
1991 MAP1B is encoded as a polyprotein: the heavy chain and light chain 1 (LC1) are translated from the same mRNA within the same open reading frame and generated by proteolytic processing. LC1 binds near the heavy-chain N-terminus and together they form a complex microtubule-binding domain. Amino acid sequencing of heavy-chain fragment, epitope mapping, Northern and Southern blotting; co-purification of heavy chain and LC1 Neuron High 1712602
1994 Light chain 3 (LC3) is a subunit of both MAP1A and MAP1B complexes; purified recombinant LC3 associates with microtubules assembled in the presence of brain MAPs and with microtubules assembled from purified tubulin, demonstrating a direct microtubule-binding activity. cDNA sequencing, microtubule cosedimentation assays with recombinant LC3, immunoprecipitation, Western blot The Journal of Biological Chemistry High 7908909
1988 MAP1B is phosphorylated in vitro by a casein kinase II (CK2)-like activity present in developing neuroblastoma cells; phosphopeptide maps of brain MAP1B phosphorylated by purified CK2 are identical to those of in vivo phosphorylated neuroblastoma MAP1B, identifying CK2 as a principal kinase acting on MAP1B during neurite outgrowth. In vitro kinase assay with purified CK2 and other kinases; phosphopeptide mapping; immunoprecipitation; microtubule cosedimentation The Journal of Cell Biology High 3164313
1992 Expression of MAP1B in fibroblasts stabilizes microtubules against depolymerizing reagents and increases alpha-tubulin acetylation, demonstrating that MAP1B promotes microtubule stability in vivo, though without the extensive microtubule bundling induced by MAP2 or tau. cDNA transfection into COS/HeLa/3T3 cells; nocodazole resistance assay; immunofluorescence for acetylated tubulin Journal of Cell Science High 1487506
1998 The MAP1B light chain (LC1), in the absence of the heavy chain, induces formation of nocodazole- and taxol-resistant stable microtubules; the heavy chain inhibits LC1 activity. LC1 contains a C-terminal actin filament-binding domain, and LC1 can dimerize/oligomerize. Heavy chain–light chain interaction domains were localized by coimmunoprecipitation of epitope-tagged fragments. Transient transfection in COS cells; immunofluorescence; nocodazole/taxol resistance assay; coimmunoprecipitation of epitope-tagged fragments; actin cosedimentation The Journal of Cell Biology High 9813091
1999 MAP1B specifically interacts with the GABA(C) receptor rho1 subunit (but not GABA(A) subunits), co-localizes with GABA(C) receptors at bipolar cell axon terminals in the retina, and redistributes rho1 upon co-expression in COS cells, suggesting MAP1B anchors GABA(C) receptors at postsynaptic sites. Yeast two-hybrid (interaction discovery), co-immunoprecipitation, immunofluorescence co-localization in retinal slices, heterologous co-expression redistribution assay in COS cells Nature High 9892354
2000 Twelve amino acids at the C-terminus of the large intracellular loop of rho1 (and rho2) are sufficient for interaction with MAP1B; disrupting the MAP1B–rho1 interaction in bipolar cells in retinal slices decreased the EC50 of GABA(C) receptors, doubling current at low GABA concentrations without affecting maximum current, demonstrating that cytoskeletal anchoring by MAP1B modulates GABA(C) receptor sensitivity. Deletion/mutagenesis mapping of rho1 interaction domain; patch-clamp electrophysiology of retinal bipolar cells in slices after disruption of MAP1B–rho interaction The Journal of Neuroscience High 11102469
1998 GSK-3β directly phosphorylates MAP1B in vitro; in cerebellar granule neurons, WNT-7a and lithium (a GSK-3β inhibitor) induce loss of the phosphorylated form of MAP1B (MAP1B-P) from axonal processes before axonal remodelling is visible, identifying MAP1B as a downstream target of the WNT–GSK-3β pathway in axonal remodelling. In vitro phosphorylation assay with purified GSK-3β and MAP1B; immunostaining of granule neurons treated with lithium or WNT-7a; time-course imaging of axonal morphology Journal of Cell Science High 9570753
2005 GSK-3β phosphorylates MAP1B at Ser1260 and Thr1265 in vitro and in vivo; phospho-specific antibodies show that GSK-3β-phosphorylated MAP1B is restricted to growing axons (distal gradient) in developing rat embryos. Site-directed mutation (Ser1260Val or Thr1265Val, or both) in full-length MAP1B alters microtubule dynamics in transfected cells, establishing these sites as a molecular switch regulating microtubule stability in growing axons. Site-directed mutagenesis of recombinant MAP1B; in vitro GSK-3β kinase assay; phospho-specific antibody generation; immunostaining of developing nervous system; heterologous cell transfection with MT dynamics assay Journal of Cell Science High 15731007
2005 Gigaxonin (GAN protein) binds MAP1B light chain (LC) through its C-terminal kelch repeat domain; gigaxonin overexpression leads to enhanced proteasome-dependent degradation of MAP1B-LC; GAN-null neurons accumulate MAP1B-LC; MAP1B overexpression causes neuronal death similar to GAN-null neurons, while MAP1B knockdown improves GAN-null neuron survival, identifying gigaxonin as a ubiquitin scaffolding protein controlling MAP1B-LC degradation and neuronal survival. Pull-down, co-immunoprecipitation; overexpression in neurons; proteasome inhibitor experiments; GAN knockout mouse neurons; MAP1B siRNA knockdown; cell viability assay Nature High 16227972
2000 MAP1B null mice (complete null allele) are viable but selectively lack the corpus callosum due to misguided cortical axons, and have reduced/thinner myelinated axons in peripheral nerves with decreased nerve conduction velocity, demonstrating an essential role for MAP1B in axon guidance and CNS/PNS development. Gene targeting (complete null allele), histology, electrophysiological nerve conduction velocity measurement, immunohistochemistry The Journal of Cell Biology High 11121433
2000 Tau and MAP1B cooperate synergistically in axonal elongation and neuronal migration: double-knockout (tau−/−map1b−/−) mice show much more severe defects (inhibited axonal elongation in hippocampal neurons, delayed neuronal migration in cerebellar neurons) than single knockouts, demonstrating functional redundancy between the two MAPs. Double-knockout mouse generation; primary hippocampal and cerebellar neuron cultures from knockout mice; morphological analysis of axon elongation and migration The Journal of Cell Biology High 10973990
2004 Netrin-1 regulates mode I MAP1B phosphorylation (activating GSK-3 and CDK5) both in vivo and in vitro; MAP1B-deficient neurons show reduced chemoattractive response to Netrin-1 in vitro, and map1b mutant mice display severe axonal tract abnormalities similar to netrin-1-deficient mice, placing MAP1B as a downstream effector in Netrin-1 signaling for axon guidance and neuronal migration. In vitro phosphorylation assays; Netrin-1 treatment of wild-type and map1b-null neurons; chemoattraction assay; analysis of map1b and netrin-1 mutant mouse brain anatomy Current Biology High 15186740
2004 Reelin induces mode I MAP1B phosphorylation through GSK-3 and CDK5 activation, with mDab1 participating in the signaling cascade; map1b-deficient mice have abnormal cortical layering consistent with a failure of neuronal migration, placing MAP1B downstream of Reelin signaling. In vitro and in vivo phosphorylation assays after Reelin treatment; analysis of map1b-null mouse brain lamination; mDab1 involvement tested biochemically Cerebral Cortex Medium 15590913
2005 The MAPK pathway (not the PI3K pathway) links NGF/TrkA receptor engagement to GSK-3β activation, which then phosphorylates MAP1B to regulate microtubule dynamics and axon growth rate; pharmacological inhibition of MAPK prevents GSK-3β activation and MAP1B phosphorylation and reduces neurite growth, while PI3K inhibition does not. Pharmacological inhibitor studies (MAPK and PI3K inhibitors) in PC12 cells and sympathetic neurons; in vitro kinase assay; GSK-3β activation assay Molecular and Cellular Neurosciences Medium 15737742
2003 NGF activates GSK-3β phosphorylation of MAP1B through TrkA receptors, not through p75NTR; BDNF (which activates p75NTR but not TrkA) does not stimulate this phosphorylation; TrkA-deficient PC12 nnr cells fail to show NGF-dependent MAP1B phosphorylation; TrkA inhibition blocks neurite elongation and MAP1B phosphorylation. Use of receptor-selective ligands and PC12 nnr cells lacking TrkA; TrkA kinase inhibitor (K252a); in vivo immunostaining for phospho-MAP1B Journal of Neurochemistry Medium 14622124
2008 DAPK-1 (death-associated protein kinase 1) binds directly to the N-terminal domain of MAP1B (residues 1–126 and a 12-aa motif); amino acid starvation induces a stable endogenous MAP1B–DAPK-1 immune complex; MAP1B is required for DAPK-1-stimulated autophagy and membrane blebbing: MAP1B siRNA attenuates these DAPK-1 activities, and MAP1B overexpression synergizes with DAPK-1 for growth inhibition. Peptide library selection; immunobinding assays; confocal co-localization; siRNA knockdown; clonogenic growth assay; autophagy and blebbing phenotype assays The Journal of Biological Chemistry Medium 18195017
2009 DYRK1A acts as a priming kinase for GSK-3β phosphorylation of MAP1B; mass spectrometry identified 28 MAP1B phosphorylation sites; DYRK1A-primed GSK-3β sites are distributed throughout the neuron while non-primed GSK-3β sites are restricted to growing axons; DYRK1A knockdown compromises neuritogenesis and alters microtubule stability. Mass spectrometry phosphosite mapping; phospho-specific antibody panel; kinase inhibitor treatments in embryonic cortical neurons; shRNA knockdown of DYRK1A; EB3 microtubule dynamics imaging Journal of Cell Science High 19549690
1993 Protein phosphatase 2A (PP2A) and PP2B (calcineurin) dephosphorylate mode I (proline-directed) MAP1B phosphorylation sites, while mode II (CK2-type) sites are dephosphorylated by PP2A and PP1 but not PP2B; inhibition of PP2A in rat brain slices (okadaic acid) increases MAP1B phosphorylation and inhibits its microtubule-binding activity. In vitro phosphatase assay with purified PP1, PP2A, PP2B; phosphorylation-state antibodies on rat brain slices treated with okadaic acid or cyclosporin A; microtubule binding assay FEBS Letters Medium 7690334
2000 PP2A is the major phosphatase regulating MAP1B phosphorylation and microtubule-binding activity in rat brain: okadaic acid (PP2A inhibitor) treatment of brain slices markedly increases MAP1B phosphorylation and inhibits MAP1B microtubule-binding activity; cyclosporin A (PP2B inhibitor) has a lesser effect. Okadaic acid and cyclosporin A treatment of metabolically active rat brain slices; Western blot with phospho-MAP1B antibodies; immunocytochemistry; microtubule binding assay Brain Research Medium 10640627
1996 Dephosphorylated MAP1B (but not native phosphorylated MAP1B) binds and cosediments with microfilaments in vitro; the proline-directed kinase (PDPK) phosphorylation site (not the CK2 sites) negatively regulates MAP1B interaction with F-actin. In vitro alkaline phosphatase dephosphorylation; F-actin cosedimentation assay; dephosphorylation kinetics correlated with F-actin binding FEBS Letters Medium 8690071
2002 MAP1B light chain 1 (LC1) binds microtubules and induces tubulin polymerization via a critical NH2-terminal microtubule-binding domain; LC1 also contains a C-terminal actin-binding domain that directly binds actin filaments; the two MAP1 light chains (LC1 of MAP1B and LC2 of MAP1A) differ in their effects on microtubule bundling and stability despite structural similarity. In vivo microtubule/actin binding assays in transfected cells; in vitro tubulin polymerization assay; domain deletion analysis; immunofluorescence The Journal of Neuroscience Medium 11896150
2007 MAP1B heavy chain directly binds actin; co-immunoprecipitation shows actin and tubulin co-precipitate with MAP1B at similar ratios throughout development regardless of phosphorylation state; atomic force microscopy measures MAP1B–actin binding force comparable to MAP1B–tubulin interaction. MAP1B heavy chain thus contains both a microtubule-stabilizing domain and an actin-binding site. Co-immunoprecipitation from brain tissue; mass spectrometry identification; atomic force microscopy force measurement; electron microscopy; COS-7 cell immunofluorescence Brain Research Bulletin Medium 17292804
2010 MAP1B deficiency reduces Rac1 and Cdc42 activity and increases RhoA activity; MAP1B interacts with Tiam1 (a Rac1 GEF); constitutively active Rac1, Cdc42, or Tiam1 rescues axon growth defects in MAP1B-deficient neurons, establishing a MAP1B–Tiam1–Rac1 axis required for microtubule–actin crosstalk during neuronal polarization. MAP1B-null mouse neurons; Rac1/Cdc42/RhoA activity pull-down assays; co-immunoprecipitation of MAP1B with Tiam1; rescue by constitutively active GTPase constructs; axon outgrowth assay Molecular Biology of the Cell High 20719958
2011 MAP1B is present in dendritic spines; MAP1B-deficient mice show decreased density of mature dendritic spines, increased filopodia-like protrusions, reduced AMPA receptor-mediated synaptic currents, decreased Rac1 activity, increased RhoA activity, and decreased phospho-cofilin in postsynaptic densities, implicating MAP1B in dendritic spine maturation via actin cytoskeleton regulation. MAP1B+/- mouse neurons; spine morphology analysis; patch-clamp electrophysiology; Rac1/RhoA activity assays; Western blot of PSD fractions The Journal of Biological Chemistry High 21984824
2013 MAP1B interacts directly with EB1 and EB3 (+TIP proteins) and sequesters them in the neuronal cytosol; MAP1B overexpression reduces EB binding to microtubule plus-ends, while MAP1B knockdown increases EB-MT association and causes microtubule overstabilization and looping in growth cones, resulting in delayed axon outgrowth. Co-immunoprecipitation; direct interaction assay; RNAi knockdown; EB3-GFP live imaging of microtubule dynamics; growth cone morphology analysis The EMBO Journal High 23572079
2011 MAP1B knockdown in embryonic rat cortical neurons decreases microtubule growth speed in the proximal and distal axon shaft (but not in growth cone filopodia) and produces more branched, slower growing axons; expression of MAP1B in MAP1B-naive cells increases microtubule elongation rate, demonstrating that MAP1B enhances microtubule assembly rates. RNAi knockdown; EB3-GFP live imaging of microtubule polymerization speed; axon morphology analysis; MAP1B expression in heterologous cells Molecular and Cellular Neurosciences High 22033417
2013 MAP1B deficiency impairs LTD expression specifically by preventing AMPA receptor endocytosis and spine shrinkage during LTD; this is due to failure of Tiam1 (Rac1 GEF) targeting to synaptic compartments and reduced Rac1 activation; providing additional Rac1 restores LTD and AMPA receptor endocytosis in MAP1B-deficient neurons, establishing a MAP1B–Tiam1–Rac1 relay for synaptic plasticity. Conditional MAP1B-deficient mouse + shRNA; electrophysiological LTD recording; AMPA receptor endocytosis assay; Tiam1 localization by immunostaining; Rac1 activity assay; rescue with Rac1 expression The EMBO Journal High 23881099
2012 Dystonin-a2 binds MAP1B in the centrosomal region; loss of this interaction in dt mutant neurons causes altered MAP1B perikaryal localization, microtubule deacetylation and instability, Golgi fragmentation, and impaired anterograde trafficking; restoring MT acetylation (trichostatin A) or MAP1B overexpression rescues these defects. Dystonin mutant mouse + isoform-specific RNAi; co-immunoprecipitation; immunofluorescence of MAP1B and acetylated tubulin; Golgi morphology; vesicle trafficking assay; rescue experiments The Journal of Cell Biology High 22412020
2012 S-nitrosylation of MAP1B light chain 1 (LC1) induces a conformational change that activates LC1 and promotes its ubiquitination by MITOL (mitochondrial ubiquitin ligase); MITOL inhibition results in accumulation of S-nitrosylated LC1, mitochondrial dysfunction, and neuronal cell death, demonstrating that MITOL regulates MAP1B-LC1 through nitrosylation-dependent ubiquitination. S-nitrosylation assay; MITOL knockdown/overexpression; ubiquitination assay; mitochondrial function assay; neuronal cell death readout; conformational change analysis Proceedings of the National Academy of Sciences USA High 22308378
2012 Syk protein-tyrosine kinase uses MAP1B as a major substrate to promote microtubule stability in MDA-MB-231 breast cancer cells; MAP1B silencing attenuates Syk-dependent microtubule acetylation and nocodazole resistance, and reverses Syk-induced changes in cell topography/stiffness measured by atomic force microscopy. Syk expression/silencing; MAP1B siRNA; nocodazole resistance assay; acetylated tubulin immunostaining; multiharmonic AFM nanomechanical mapping Biochemistry Medium 24914616
2012 Nav1.6 (SCN8A) voltage-gated sodium channel N-terminus interacts with MAP1B light chain via residues 77–80 (VAVP) of Nav1.6; co-expression of Nav1.6 with Map1b in ND7/23 neuronal cells increases sodium current density 50%; mutation of the Map1b-binding site of Nav1.6 prevents generation of sodium current, demonstrating that MAP1B facilitates Nav1.6 trafficking to the neuronal cell surface. Yeast two-hybrid screen; co-immunoprecipitation from mouse brain; alanine-scanning mutagenesis; patch-clamp electrophysiology in transfected cells The Journal of Biological Chemistry High 22474336
2009 Nemo-like kinase (NLK) directly phosphorylates MAP1B in vitro; NGF promotes NLK translocation to leading edges of PC12 cells and activates NLK kinase activity; NLK knockdown reduces MAP1B phosphorylation and inhibits NGF-induced F-actin redistribution and neurite outgrowth. In vitro kinase assay with purified NLK and MAP1B; NLK knockdown; immunofluorescence of F-actin and NLK; neurite outgrowth assay Journal of Neurochemistry Medium 19840224
2006 QKI RNA-binding protein binds the 3'UTR of MAP1B mRNA in oligodendroglia; QKI-deficiency (quakingviable mice) reduces MAP1B mRNA expression; QKI knockdown destabilizes MAP1B mRNA in CG4 cells; forced QKI expression is sufficient to promote MAP1B expression, demonstrating QKI-dependent mRNA stabilization as a post-transcriptional mechanism controlling MAP1B levels specifically in oligodendroglia. 3'UTR binding assay; qv mutant mice analysis; RNAi knockdown of QKI; QKI overexpression; Northern blot / qPCR for MAP1B mRNA stability Molecular Biology of the Cell Medium 16855020
2011 Staufen 2 (Stau2) knockdown reduces dendritic localization of Map1b mRNA, decreases basal Map1b protein in dendrites, and prevents mGluR/DHPG-induced increases in dendritic Map1b protein; Stau2 is required for mGluR-LTD (but not LTP); mGluR stimulation induces Map1b mRNA dissociation from Stau2/P0-containing granules, demonstrating Stau2 controls Map1b mRNA dendritic distribution and translation for mGluR-LTD. Stau2 shRNA knockdown; Map1b mRNA FISH; protein immunostaining in dendrites; electrophysiology (LTP, LTD); granule co-localization; DHPG stimulation Learning & Memory Medium 21508097
2012 In growth cones, a DLK–MKK7–JNK1 MAP kinase module phosphorylates Map1b to regulate microtubule bundling and neurite elongation; MKK7 mRNA localizes to the growth cone and can be locally translated there; disruption of this pathway alters Map1b phosphorylation and microtubule bundling. Genome-wide mRNA localization screen; MKK7 mRNA FISH in growth cones; phospho-Map1b immunostaining; kinase inhibitor and dominant-negative experiments; neurite elongation assay PLoS Biology Medium 23226105
2007 MAP1B coordinates microtubule and actin cytoskeleton remodeling; MAP1B is required for LPA-induced microtubule backfolding during process retraction in DRG neurons and Schwann cells; MAP1B-deficient cells show actin contraction but fail to execute the subsequent microtubule backfolding step, and MAP1B is required for Schwann cell migration in vitro. Map1b-null mouse neurons and Schwann cells; LPA stimulation; time-lapse imaging of cytoskeletal rearrangements; migration assay Molecular and Cellular Neurosciences Medium 17764972
2016 GSK-3-mediated MAP1B phosphorylation is locally reduced at neurite branching points; MAP1B is required downstream of GSK-3 for branching control, as map1b−/− neurons are not affected by GSK-3 inhibition and re-expression of MAP1B in map1b−/− neurons restores wild-type branching. Phospho-MAP1B preferentially associates with tyrosinated microtubules and its dephosphorylation by GSK-3 inhibition protects both tyrosinated and acetylated MTs from nocodazole depolymerization. Map1b-null mouse neurons; GSK-3 inhibitor treatments; cDNA rescue transfection; MAP1B-transfected fibroblasts + nocodazole assay; phospho-MAP1B immunostaining at branch points; tyrosinated/acetylated tubulin staining Molecular and Cellular Neurosciences Medium 26773468
2015 FMRP associates with miR-181d, Map1b mRNA, and Calm1 mRNA in axons; miR-181d delivered by FMRP negatively regulates local translation of MAP1B in axons; FMRP deficiency (Fmr1I304N or Fmr1 knockdown) impedes axonal delivery of miR-181d and Map1b mRNA, reducing MAP1B protein in axons; NGF releases Map1b mRNA from FMRP/miR-181d-repressing granules to promote axon elongation. FMRP co-immunoprecipitation with miR-181d and Map1b mRNA; microfluidic axon isolation; MAP1B protein quantification in axons; NGF stimulation; Fmr1 mutant mice Cell Reports Medium 26711345
2019 In SNCA-A53T (Parkinson's disease) human neurons, mutant α-synuclein fails to complex with PKC, impairing Nrf2 activation; reduced Nrf2 activity on antioxidant response elements (AREs) at the Map1b gene enhancer decreases MAP1B expression; forced MAP1B expression or Nrf2 activation rescues neuritic length/complexity defects in PD neurons. hPSC-derived A9-type dopaminergic neurons + isogenic controls; ChIP-seq/reporter assay for Nrf2 on Map1b ARE; MAP1B overexpression rescue; Nrf2 pharmaceutical activation; neuritic morphology analysis Proceedings of the National Academy of Sciences USA Medium 31235589
2016 MAP1B-deficient neurons show decreased density of presynaptic terminals, increased proportion of orphan presynaptic terminals, altered synaptic vesicle fusion (FM4-64 assay), and decreased density of synaptic vesicles and dense core vesicles at presynaptic terminals, identifying a presynaptic structural and functional role for MAP1B. MAP1B KO mouse neurons; immunofluorescence quantification of synaptic terminal density; FM4-64 synaptic vesicle fusion assay; electron microscopy of presynaptic terminals Scientific Reports Medium 27425640
2020 MAP1B mutations (c.4198A>G p.S1400G; c.2768T>C p.I923T; c.5512T>C p.F1838L) cause reduced MAP1B levels and deficient MAP1B phosphorylation in patient-derived otic sensory neuron-like cells; these cells exhibit disturbed microtubule dynamics, impaired axonal elongation, and electrophysiological defects, all rescued by CRISPR/Cas9 correction of the MAP1B mutation; Map1b+/- mice show progressive hearing loss with spiral ganglion neuron microtubule phosphorylation defects. Patient iPSC-derived otic neurons; CRISPR/Cas9 correction; Map1b+/- mouse audiometry; MAP1B phosphorylation Western blot; microtubule dynamics imaging; patch-clamp electrophysiology JCI Insight High 33268592

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 Drosophila fragile X-related gene regulates the MAP1B homolog Futsch to control synaptic structure and function. Cell 547 11733059
2000 Drosophila Futsch/22C10 is a MAP1B-like protein required for dendritic and axonal development. Neuron 377 10839355
2000 Defects in axonal elongation and neuronal migration in mice with disrupted tau and map1b genes. The Journal of cell biology 331 10973990
1989 The microtubule binding domain of microtubule-associated protein MAP1B contains a repeated sequence motif unrelated to that of MAP2 and tau. The Journal of cell biology 297 2480963
1992 Increased microtubule stability and alpha tubulin acetylation in cells transfected with microtubule-associated proteins MAP1B, MAP2 or tau. Journal of cell science 292 1487506
1998 Inhibition of GSK-3beta leading to the loss of phosphorylated MAP-1B is an early event in axonal remodelling induced by WNT-7a or lithium. Journal of cell science 260 9570753
2014 Activity-associated miRNA are packaged in Map1b-enriched exosomes released from depolarized neurons. Nucleic acids research 241 25053844
1994 Molecular characterization of light chain 3. A microtubule binding subunit of MAP1A and MAP1B. The Journal of biological chemistry 239 7908909
2012 microRNA-9 regulates axon extension and branching by targeting Map1b in mouse cortical neurons. Nature neuroscience 232 22484572
1986 MAP5: a novel brain microtubule-associated protein under strong developmental regulation. Journal of neurocytology 219 3819779
1991 Abnormal expression of two microtubule-associated proteins (MAP2 and MAP5) in specific subfields of the hippocampal formation in schizophrenia. Proceedings of the National Academy of Sciences of the United States of America 211 1961755
2000 MAP1B is required for axon guidance and Is involved in the development of the central and peripheral nervous system. The Journal of cell biology 177 11121433
1988 A casein kinase II-related activity is involved in phosphorylation of microtubule-associated protein MAP-1B during neuroblastoma cell differentiation. The Journal of cell biology 162 3164313
1994 Microtubule-associated protein 1b (MAP1b) is concentrated in the distal region of growing axons. The Journal of neuroscience : the official journal of the Society for Neuroscience 150 8301365
2005 Glycogen synthase kinase-3beta phosphorylation of MAP1B at Ser1260 and Thr1265 is spatially restricted to growing axons. Journal of cell science 137 15731007
1990 Molecular cloning of microtubule-associated protein 1 (MAP1A) and microtubule-associated protein 5 (MAP1B): identification of distinct genes and their differential expression in developing brain. Journal of neurochemistry 137 2355215
1998 Novel features of the light chain of microtubule-associated protein MAP1B: microtubule stabilization, self interaction, actin filament binding, and regulation by the heavy chain. The Journal of cell biology 132 9813091
2014 Futsch/MAP1B mRNA is a translational target of TDP-43 and is neuroprotective in a Drosophila model of amyotrophic lateral sclerosis. The Journal of neuroscience : the official journal of the Society for Neuroscience 126 25429138
2008 DAPK-1 binding to a linear peptide motif in MAP1B stimulates autophagy and membrane blebbing. The Journal of biological chemistry 115 18195017
2005 Gigaxonin-controlled degradation of MAP1B light chain is critical to neuronal survival. Nature 114 16227972
1997 Delayed development of nervous system in mice homozygous for disrupted microtubule-associated protein 1B (MAP1B) gene. The Journal of cell biology 112 9199175
1999 The protein MAP-1B links GABA(C) receptors to the cytoskeleton at retinal synapses. Nature 111 9892354
1990 Immunochemical evidence that fragments of phosphorylated MAP5 (MAP1B) are bound to neurofibrillary tangles in Alzheimer's disease. Neuron 110 2361014
2011 TDP-43 regulates Drosophila neuromuscular junctions growth by modulating Futsch/MAP1B levels and synaptic microtubules organization. PloS one 108 21412434
2000 MAP1B expression and microtubule stability in growing and regenerating axons. Microscopy research and technique 108 10649507
2005 Microtubule-associated protein MAP1A, MAP1B, and MAP2 proteolysis during soluble amyloid beta-peptide-induced neuronal apoptosis. Synergistic involvement of calpain and caspase-3. The Journal of biological chemistry 103 16234245
2004 MAP1B is required for Netrin 1 signaling in neuronal migration and axonal guidance. Current biology : CB 102 15186740
2014 The MAP1B case: an old MAP that is new again. Developmental neurobiology 101 24700609
1991 MAP1B is encoded as a polyprotein that is processed to form a complex N-terminal microtubule-binding domain. Neuron 101 1712602
1990 Changes in microtubule-associated protein MAP1B phosphorylation during rat brain development. Journal of neurochemistry 99 1693946
1994 Respective roles of neurofilaments, microtubules, MAP1B, and tau in neurite outgrowth and stabilization. Molecular biology of the cell 95 7803854
1993 Heterogeneity in the phosphorylation of microtubule-associated protein MAP1B during rat brain development. Journal of neurochemistry 92 7689645
2004 A role of MAP1B in Reelin-dependent neuronal migration. Cerebral cortex (New York, N.Y. : 1991) 91 15590913
2005 The MAP kinase pathway is upstream of the activation of GSK3beta that enables it to phosphorylate MAP1B and contributes to the stimulation of axon growth. Molecular and cellular neurosciences 88 15737742
2009 Nonprimed and DYRK1A-primed GSK3 beta-phosphorylation sites on MAP1B regulate microtubule dynamics in growing axons. Journal of cell science 84 19549690
1997 Internexin, MAP1B, and nestin in cortical dysplasia as markers of developmental maturity. Acta neuropathologica 83 9194902
2002 Microtubule-associated protein 1A (MAP1A) and MAP1B: light chains determine distinct functional properties. The Journal of neuroscience : the official journal of the Society for Neuroscience 81 11896150
2011 Microtubule-associated protein 1B (MAP1B) is required for dendritic spine development and synaptic maturation. The Journal of biological chemistry 80 21984824
2010 MAP1B regulates axonal development by modulating Rho-GTPase Rac1 activity. Molecular biology of the cell 79 20719958
2005 Disruption of the MAP1B-related protein FUTSCH leads to changes in the neuronal cytoskeleton, axonal transport defects, and progressive neurodegeneration in Drosophila. Molecular biology of the cell 79 15772149
2000 The microtubule-associated protein MAP1B is involved in local stabilization of turning growth cones. Molecular and cellular neurosciences 79 10662505
1993 Refinement of the spinal muscular atrophy locus to the interval between D5S435 and MAP1B. Genomics 74 8449502
1989 Cloning of a cDNA encoding MAP1B in rat brain: regulation of mRNA levels during development. Journal of neurochemistry 74 2723641
2014 Neuronal deletion of GSK3β increases microtubule speed in the growth cone and enhances axon regeneration via CRMP-2 and independently of MAP1B and CLASP2. BMC biology 73 24923837
2013 MAP1B regulates microtubule dynamics by sequestering EB1/3 in the cytosol of developing neuronal cells. The EMBO journal 73 23572079
2000 Regulation of phosphorylation of neuronal microtubule-associated proteins MAP1b and MAP2 by protein phosphatase-2A and -2B in rat brain. Brain research 68 10640627
2015 FMRP-Mediated Axonal Delivery of miR-181d Regulates Axon Elongation by Locally Targeting Map1b and Calm1. Cell reports 65 26711345
2011 MAP1B enhances microtubule assembly rates and axon extension rates in developing neurons. Molecular and cellular neurosciences 64 22033417
1993 Microtubule-associated protein MAP1B expression precedes the morphological differentiation of oligodendrocytes. Journal of neuroscience research 63 8350387
1990 Microtubule associated protein (MAP1B) is present in cultured oligodendrocytes and co-localizes with tubulin. Journal of neuroscience research 62 2254953
1996 Expression of a phosphorylated isoform of MAP1B is maintained in adult central nervous system areas that retain capacity for structural plasticity. The Journal of comparative neurology 61 8725342
1994 Microtubule-associated protein MAP1B showing a fetal phosphorylation pattern is present in sites of neurofibrillary degeneration in brains of Alzheimer's disease patients. Brain research. Molecular brain research 61 7854037
1991 Specific association of an M-phase kinase with isolated mitotic spindles and identification of two of its substrates as MAP4 and MAP1B. Cell regulation 61 1667090
1996 Dephosphorylated but not phosphorylated microtubule associated protein MAP1B binds to microfilaments. FEBS letters 60 8690071
2006 QKI binds MAP1B mRNA and enhances MAP1B expression during oligodendrocyte development. Molecular biology of the cell 58 16855020
2001 Expression of QKI proteins and MAP1B identifies actively myelinating oligodendrocytes in adult rat brain. Molecular and cellular neurosciences 57 11178867
2012 Microtubule stability, Golgi organization, and transport flux require dystonin-a2-MAP1B interaction. The Journal of cell biology 56 22412020
2009 Bcl11A/CTIP1 regulates expression of DCC and MAP1b in control of axon branching and dendrite outgrowth. Molecular and cellular neurosciences 56 19616629
1999 Upregulation of MAP1B and MAP2 in the rat brain after middle cerebral artery occlusion: effect of age. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 56 10197512
2011 Staufen 2 regulates mGluR long-term depression and Map1b mRNA distribution in hippocampal neurons. Learning & memory (Cold Spring Harbor, N.Y.) 55 21508097
2012 Mitochondrial ubiquitin ligase MITOL blocks S-nitrosylated MAP1B-light chain 1-mediated mitochondrial dysfunction and neuronal cell death. Proceedings of the National Academy of Sciences of the United States of America 54 22308378
2018 De novo and inherited private variants in MAP1B in periventricular nodular heterotopia. PLoS genetics 53 29738522
1996 Differences in the regulation of microtubule dynamics by microtubule-associated proteins MAP1B and MAP2. Cell motility and the cytoskeleton 53 8894283
1995 Expression and distribution of phosphorylated MAP1B in growing axons of cultured hippocampal neurons. Journal of neuroscience research 52 7616605
2016 The GSK3-MAP1B pathway controls neurite branching and microtubule dynamics. Molecular and cellular neurosciences 51 26773468
2013 Modulation of mGluR-dependent MAP1B translation and AMPA receptor endocytosis by microRNA miR-146a-5p. The Journal of neuroscience : the official journal of the Society for Neuroscience 51 23699512
1987 Developmental regulation of two microtubule-associated proteins (MAP2 and MAP5) in the embryonic avian retina. Development (Cambridge, England) 49 3502994
1996 Gene localization and developmental expression of light chain 3: a common subunit of microtubule-associated protein 1A(MAP1A) and MAP1B. Journal of neuroscience research 48 8833088
1994 Postmortem changes in the levels and localization of microtubule-associated proteins (tau, MAP2 and MAP1B) in the rat and human hippocampus. Hippocampus 48 7951696
1993 Dephosphorylation of distinct sites on microtubule-associated protein MAP1B by protein phosphatases 1, 2A and 2B. FEBS letters 48 7690334
2015 Autism and Intellectual Disability-Associated KIRREL3 Interacts with Neuronal Proteins MAP1B and MYO16 with Potential Roles in Neurodevelopment. PloS one 47 25902260
1993 Distribution of microtubule-associated proteins (MAPs) in adult and embryonic mouse retinal explants: presence of the embryonic map, MAP5/1B, in regenerating adult retinal axons. Developmental biology 46 8432404
2006 The Drosophila microtubule associated protein Futsch is phosphorylated by Shaggy/Zeste-white 3 at an homologous GSK3beta phosphorylation site in MAP1B. Molecular and cellular neurosciences 44 16949836
2000 GABAC receptor sensitivity is modulated by interaction with MAP1B. The Journal of neuroscience : the official journal of the Society for Neuroscience 44 11102469
2012 Growth cone MKK7 mRNA targeting regulates MAP1b-dependent microtubule bundling to control neurite elongation. PLoS biology 43 23226105
2013 MAP1B-dependent Rac activation is required for AMPA receptor endocytosis during long-term depression. The EMBO journal 42 23881099
1994 Claustrin, an antiadhesive neural keratan sulfate proteoglycan, is structurally related to MAP1B. Journal of neurobiology 42 7906711
2007 MAP1B coordinates microtubule and actin filament remodeling in adult mouse Schwann cell tips and DRG neuron growth cones. Molecular and cellular neurosciences 41 17764972
1992 Fine-mapping of the spinal muscular atrophy locus to a region flanked by MAP1B and D5S6. Genomics 41 1505990
2015 Epigenetic Repression of CCDC37 and MAP1B Links Chronic Obstructive Pulmonary Disease to Lung Cancer. Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 39 26200272
1990 The expression of phosphorylated and non-phosphorylated forms of MAP5 in the amphibian CNS. Brain research 39 2306617
1989 Microtubule-associated proteins MAP5 and MAP1x: closely related components of the neuronal cytoskeleton with different cytoplasmic distributions in the developing brain. Brain research. Molecular brain research 38 2927285
1997 Age-dependent organotypic expression of microtubule-associated proteins (MAP1, MAP2, and MAP5) in rat brain. Neurochemical research 37 9178955
1989 Characterization of proteins immunologically related to brain microtubule-associated protein MAP-1B in non-neural cells. Journal of cell science 37 2689462
2016 Microtubule-associated protein 1B (MAP1B)-deficient neurons show structural presynaptic deficiencies in vitro and altered presynaptic physiology. Scientific reports 36 27425640
2003 Joint regulation of the MAP1B promoter by HNF3beta/Foxa2 and Engrailed is the result of a highly conserved mechanism for direct interaction of homeoproteins and Fox transcription factors. Development (Cambridge, England) 36 12642491
2003 NGF activates the phosphorylation of MAP1B by GSK3beta through the TrkA receptor and not the p75(NTR) receptor. Journal of neurochemistry 36 14622124
1998 The mouse and rat MAP1B genes: genomic organization and alternative transcription. Genomics 36 9615228
2020 Mutations of MAP1B encoding a microtubule-associated phosphoprotein cause sensorineural hearing loss. JCI insight 35 33268592
2014 Nanomechanical property maps of breast cancer cells as determined by multiharmonic atomic force microscopy reveal Syk-dependent changes in microtubule stability mediated by MAP1B. Biochemistry 35 24914616
1995 Microtubule associated protein 1B (MAP1B) promotes efficient tubulin polymerisation in vitro. FEBS letters 35 7664878
1991 Association of microtubule-associated protein (MAP1B) with growing axons in cultured hippocampal neurons. Molecular and cellular neurosciences 35 19912782
2007 Characterization of MAP1B heavy chain interaction with actin. Brain research bulletin 34 17292804
1997 Differential regulation of microtubule-associated protein 1B (MAP1B) in rat CNS and PNS during development. Journal of neuroscience research 34 9260743
2018 MAP1B mutations cause intellectual disability and extensive white matter deficit. Nature communications 33 30150678
1996 Characterization of microtubule-associated protein MAP1B: phosphorylation state, light chains, and binding to microtubules. Biochemistry 33 8608140
1992 Immunohistochemical expression of microtubule-associated protein 5 (MAP5) in glial cells in multiple system atrophy. Journal of the neurological sciences 33 1517758
2012 Interaction of voltage-gated sodium channel Nav1.6 (SCN8A) with microtubule-associated protein Map1b. The Journal of biological chemistry 32 22474336
2009 Nemo-like kinase is involved in NGF-induced neurite outgrowth via phosphorylating MAP1B and paxillin. Journal of neurochemistry 32 19840224
2019 Axonal pathology in hPSC-based models of Parkinson's disease results from loss of Nrf2 transcriptional activity at the Map1b gene locus. Proceedings of the National Academy of Sciences of the United States of America 31 31235589

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