Affinage

MAGI2

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 · UniProt Q86UL8

Length
1455 aa
Mass
158.8 kDa
Annotated
2026-04-28
100 papers in source corpus 19 papers cited in narrative 19 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

MAGI2 is a multi-PDZ domain scaffold protein that organizes signaling complexes at cell junctions and synapses, functioning in epithelial barrier integrity, synaptic receptor maintenance, and tumor suppression. At adherens junctions and the kidney slit diaphragm, MAGI2 stabilizes PTEN protein (via PDZ2-mediated binding regulated by PTEN C-terminal phosphorylation) to suppress PI3K-Akt signaling, and scaffolds nephrin and RapGEF2 to activate Rap1 GTPase signaling essential for podocyte cytoskeletal integrity; loss-of-function mutations in MAGI2 cause congenital nephrotic syndrome in humans (PMID:10760291, PMID:11431330, PMID:25271328, PMID:31171376, PMID:27932480). At synapses, MAGI2 interacts with TARPs/stargazin, neuroligin-2, and β-dystroglycan to maintain surface AMPA receptor pools and dendritic spine density in a GluA2-dependent, activity-independent manner, and its hemizygous deletion is associated with infantile spasms (PMID:22593065, PMID:16870733, PMID:17059560, PMID:18565486). MAGI2 is also targeted for proteasomal degradation by high-risk HPV E6 proteins and is subject to miRNA-mediated downregulation, linking its loss to epithelial-mesenchymal transition and drug resistance in cancer (PMID:12140759, PMID:24258346).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 2000 High

    Identifying how PTEN is stabilized at cell membranes, this work established that MAGI2 directly binds PTEN through a PDZ2–PDZ-binding motif interaction, enhancing PTEN-mediated Akt suppression and defining MAGI2 as a PTEN scaffold.

    Evidence Yeast two-hybrid, co-immunoprecipitation, and functional Akt suppression assays in mammalian cells

    PMID:10760291

    Open questions at the time
    • Structural basis of PDZ2–PTEN interaction unresolved
    • In vivo significance of MAGI2-PTEN axis not yet tested in animal models
  2. 2001 High

    Addressing how the MAGI2–PTEN interaction is regulated, phosphorylation at PTEN T382/T383 was shown to control binding affinity to MAGI2 — dephosphorylated PTEN binds MAGI2 more tightly but becomes susceptible to proteasomal degradation, revealing a phosphorylation-dependent switch governing PTEN junctional recruitment.

    Evidence Site-directed mutagenesis, pulldown assays, and ubiquitination assays

    PMID:11431330

    Open questions at the time
    • Kinase(s) responsible for T382/T383 phosphorylation in vivo not identified
    • Whether this regulation operates at specific junction types unclear
  3. 2001 High

    Extending MAGI2's scaffolding repertoire beyond PTEN, its PDZ1 domain was shown to bind the β1-adrenergic receptor C-terminus in an agonist-enhanced manner, promoting receptor internalization and linking MAGI2 to GPCR trafficking.

    Evidence Overlay assays, pulldown, co-immunoprecipitation, and immunofluorescence in transfected cells

    PMID:11526121

    Open questions at the time
    • Physiological significance of MAGI2–β1AR interaction in cardiac tissue not tested
    • Whether MAGI2 scaffolds other GPCRs unknown
  4. 2002 Medium

    Revealing a mechanism by which HPV subverts cell polarity, high-risk HPV E6 proteins were shown to target MAGI2 for proteasome-mediated degradation via a specific PDZ domain interaction, which could be blocked by competitive expression of that domain.

    Evidence Co-expression and degradation assays with domain competition in cultured cells

    PMID:12140759

    Open questions at the time
    • In vivo relevance during HPV-driven carcinogenesis not demonstrated
    • Which E3 ligase mediates E6-directed MAGI2 ubiquitination not identified
  5. 2004 High

    Establishing the junctional pathway that stabilizes PTEN, vinculin was shown to maintain the β-catenin–MAGI2 interaction at adherens junctions; loss of vinculin disrupts this complex, causing PTEN destabilization and degradation.

    Evidence Genetic rescue in vinculin-null cells, co-immunoprecipitation, and MAGI2 overexpression rescue

    PMID:15579911

    Open questions at the time
    • Direct vs. indirect nature of vinculin's effect on MAGI2 assembly unclear
    • Whether this pathway operates in all epithelial tissues not tested
  6. 2005 High

    Identifying MAGI2 as a slit diaphragm component, it was found in the nephrin multiprotein complex at glomerular podocyte foot processes, establishing a non-neuronal scaffolding role for MAGI2 in kidney filtration.

    Evidence GST-nephrin pulldown with mass spectrometry and immunofluorescence co-localization in kidney sections

    PMID:15994232

    Open questions at the time
    • Functional consequence of MAGI2 loss in podocytes not yet shown
    • Binding domains mediating MAGI2–nephrin interaction not mapped
  7. 2006 High

    Defining MAGI2's synaptic scaffolding architecture, two key interactions were mapped: MAGI2 bridges β-dystroglycan (via WW domains) and neuroligin-2 (via PDZ2) into a tripartite complex at inhibitory synapses, and separately interacts with stargazin/TARPs (via PDZ1/3/5) to scaffold AMPA receptor complexes at excitatory synapses.

    Evidence Yeast two-hybrid, co-immunoprecipitation from rodent brain, in vitro complex reconstitution, domain mapping in HEK-293T cells

    PMID:16870733 PMID:17059560

    Open questions at the time
    • Functional consequence of disrupting the tripartite complex at inhibitory synapses not tested
    • Whether MAGI2 simultaneously engages all these partners in a single complex unknown
  8. 2008 Medium

    Linking MAGI2 to human neurological disease, hemizygous MAGI2 deletion was identified in patients with infantile spasms, consistent with its role in scaffolding stargazin/epilepsy-associated proteins.

    Evidence High-resolution genomic mapping of chromosomal deletions in patients with infantile spasms

    PMID:18565486

    Open questions at the time
    • Causal role not proven by functional rescue
    • Whether MAGI2 haploinsufficiency alone is sufficient for epilepsy or requires additional hits unclear
  9. 2012 High

    Resolving the functional role of MAGI2 at excitatory synapses, bidirectional manipulation showed that MAGI2 is essential for maintaining surface GluA2-containing AMPA receptors and dendritic spine density, and its overexpression blocks NMDA-induced AMPAR internalization and LTD.

    Evidence RNAi knockdown and overexpression in rat hippocampal neurons with electrophysiology, spine imaging, and surface receptor quantification

    PMID:22593065

    Open questions at the time
    • Whether MAGI2 regulation of AMPARs involves direct GluA2 binding or is entirely TARP-mediated not fully resolved
    • In vivo behavioral consequences of synaptic MAGI2 loss not tested
  10. 2014 High

    Demonstrating MAGI2's essential role in kidney filtration, two independent MAGI2-knockout mouse studies showed progressive proteinuria, podocyte foot process effacement, loss of nephrin and dendrin from slit diaphragms, dendrin nuclear translocation, upregulated cathepsin L, and renal failure, establishing MAGI2 as indispensable for podocyte integrity.

    Evidence Homologous recombination knockout mice analyzed by electron microscopy, immunohistochemistry, and proteinuria measurement

    PMID:25108225 PMID:25271328

    Open questions at the time
    • Molecular mechanism linking MAGI2 loss to dendrin nuclear translocation not defined
    • Whether MAGI2 loss causes a primary signaling defect or structural disassembly is unclear
  11. 2014 High

    Connecting MAGI2 to sensory cell function, CK2 phosphorylation of the Usher syndrome protein SANS was shown to regulate SANS–MAGI2 complex assembly, controlling MAGI2-mediated clathrin-dependent endocytosis required for ciliogenesis; patient mutations in SANS that abolish MAGI2 binding deregulate endocytosis and disrupt photoreceptor function.

    Evidence Co-immunoprecipitation, CK2 kinase assays, endocytosis assays, patient mutation analysis in retinal photoreceptor cells

    PMID:24608321

    Open questions at the time
    • Whether MAGI2 directly participates in clathrin coat assembly or acts indirectly through cargo sorting not resolved
    • Role of MAGI2 in other ciliated cell types not tested
  12. 2016 Medium

    Confirming clinical relevance, MAGI2 loss-of-function mutations (frameshift, duplication) were identified as a cause of congenital nephrotic syndrome in humans, with absent MAGI2 protein in patient kidney tissue.

    Evidence Whole-exome sequencing of patients with congenital nephrotic syndrome and immunohistochemistry of patient kidneys

    PMID:27932480

    Open questions at the time
    • Number of families studied is small
    • Genotype-phenotype correlation across different MAGI2 mutations not established
  13. 2019 High

    Identifying the signaling pathway downstream of MAGI2 in podocytes, MAGI2 was shown to form a complex with RapGEF2 to activate Rap1 GTPase; disease-causing MAGI2 mutations disrupt this activation, podocyte-specific RapGEF2 deletion phenocopies MAGI2 loss, and pharmacological Rap1 activation rescues actin defects caused by MAGI2 mutations.

    Evidence Co-immunoprecipitation, Rap1 activation assays, podocyte-specific conditional knockout mice, pharmacological rescue in human podocytes

    PMID:31171376

    Open questions at the time
    • Whether the MAGI2–RapGEF2–Rap1 axis operates in neurons or other cell types unknown
    • Structural basis of MAGI2–RapGEF2 interaction not determined

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include how MAGI2 coordinates its multiple PDZ domain interactions simultaneously, whether the PTEN-stabilizing and Rap1-activating functions are integrated or independent at podocyte junctions, and what molecular mechanisms link MAGI2 haploinsufficiency to epileptogenesis in humans.
  • No structural model of full-length MAGI2 or multi-partner complexes
  • Relative contribution of PTEN stabilization vs. Rap1 activation to podocyte survival not dissected
  • Causal mechanism linking MAGI2 loss to infantile spasms not functionally validated

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 6
Localization
GO:0005886 plasma membrane 7
Pathway
R-HSA-1500931 Cell-Cell communication 4 R-HSA-162582 Signal Transduction 4 R-HSA-112316 Neuronal System 3 R-HSA-1266738 Developmental Biology 3 GO:0005886 plasma membrane 1
Partners
CACNG2CTNNB1DAG1NLGN2NPHS1PTENRAPGEF2USH1G
Complex memberships
Nephrin slit diaphragm complexTARP/stargazin–AMPAR complexβ-dystroglycan–neuroligin-2 complex

Evidence

Reading pass · 19 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 MAGI2 (renamed from AIP-1/atrophin interacting protein 1) binds PTEN through an interaction between the PDZ-binding motif of PTEN and the second PDZ domain of MAGI2, and this interaction enhances PTEN's ability to suppress Akt activation and stabilizes PTEN protein at the cell membrane. Yeast two-hybrid screening, co-immunoprecipitation, functional Akt suppression assay Proceedings of the National Academy of Sciences of the United States of America High 10760291
2001 PTEN binding to MAGI2 is regulated by phosphorylation at threonine 382 and 383 of PTEN; phosphorylation-incompetent PTEN mutants (T382A/T383A) show dramatically enhanced binding affinity to MAGI2 but reduced protein stability via proteasome-dependent polyubiquitination, suggesting PTEN recruitment to cell-cell junctions is regulated through C-terminal phosphorylation. Site-directed mutagenesis, pulldown assay, ubiquitination assay Cancer research High 11431330
2001 MAGI2 binds the beta1-adrenergic receptor (beta1AR) carboxyl terminus through its first PDZ domain, with the last few amino acids of the beta1AR C-terminus being the key determinants; this interaction is enhanced by agonist stimulation and markedly increases beta1AR internalization. MAGI2 also promotes physical association of beta1AR with beta-catenin. Overlay and pulldown assays, co-immunoprecipitation, immunofluorescence co-localization, domain-specific binding assays The Journal of biological chemistry High 11526121
2002 High-risk HPV E6 proteins target MAGI2 for proteasome-mediated degradation through interaction with a specific PDZ domain of MAGI2; co-expression of this PDZ domain can protect full-length MAGI2 from E6-mediated degradation. Co-expression and degradation assays, domain competition experiments Oncogene Medium 12140759
2004 Vinculin maintains PTEN protein levels by preserving the interaction of beta-catenin with MAGI2 at adherens junctions; loss of vinculin disrupts the beta-catenin–MAGI2 interaction, leading to PTEN destabilization and degradation. Genetic rescue experiments (vinculin-null cells), co-immunoprecipitation, transfection with vinculin mutants and E-cadherin fusion constructs, MAGI2 overexpression rescue The Journal of biological chemistry High 15579911
2005 MAGI2 (S-SCAM) is a component of the nephrin multiprotein complex at glomerular slit diaphragms; it is pulled down by the GST-nephrin cytoplasmic domain from glomerular lysates and co-localizes with nephrin in podocyte foot processes. GST pulldown with mass spectrometry, immunofluorescence co-localization Proceedings of the National Academy of Sciences of the United States of America High 15994232
2003 PKC-mediated phosphorylation of the delta2 glutamate receptor regulates its interaction with MAGI2 (S-SCAM), and co-expression of delta2 receptor with MAGI2 dramatically alters receptor localization in cells. Co-immunoprecipitation, co-expression in COS7 cells, immunolocalization Biochemical and biophysical research communications Medium 12589829
2006 MAGI2 (S-SCAM) localizes at inhibitory synapses and interacts with beta-dystroglycan (beta-DG) through its WW domains; MAGI2 WW domains and its second PDZ domain interact with neuroligin 2. Beta-DG, neuroligin 2, and MAGI2 form a tripartite complex in vitro, linking the dystrophin glycoprotein complex to the neurexin-neuroligin complex at inhibitory synapses. Yeast two-hybrid, co-immunoprecipitation from rat brain, in vitro complex reconstitution, immunofluorescence in hippocampal neurons Journal of neurochemistry High 17059560
2006 MAGI2 interacts with stargazin and other TARPs (transmembrane AMPA receptor regulating proteins) via the C-terminal -TTPV motif of stargazin and the PDZ1, PDZ3, and PDZ5 domains of MAGI2; MAGI2 co-immunoprecipitates with stargazin in vivo from mouse cerebral cortex and stargazin expression recruits MAGI2 to cell membranes. Yeast two-hybrid, co-immunoprecipitation from mouse brain, in vitro domain mapping, co-expression in HEK-293T cells The Journal of neuroscience High 16870733
2007 MAGI2 overexpression inhibits cell migration and proliferation in hepatocarcinoma cells by upregulating PTEN protein stability (not mRNA), leading to downregulation of p-FAK and p-Akt; the effect is attenuated in PTEN-null cells and rescued by PTEN transfection, confirming PTEN-dependent mechanism. Transfection/overexpression, migration and proliferation assays, western blot, siRNA knockdown of PTEN, co-immunoprecipitation Archives of biochemistry and biophysics Medium 17880912
2008 Hemizygous deletion within the MAGI2 gene (encoding the synaptic scaffolding protein) is associated with infantile spasms; MAGI2 interacts with Stargazin, a protein associated with epilepsy in the stargazer mouse. High-resolution genomic mapping of chromosomal deletions in patients, candidate gene analysis American journal of human genetics Medium 18565486
2012 MAGI2 (S-SCAM) is an essential synaptic scaffolding molecule for the GluA2-containing maintenance pool of AMPA receptors; increasing MAGI2 levels selectively increases surface AMPAR levels and enlarges dendritic spines, while MAGI2 knockdown causes loss of synaptic AMPARs and severe reduction in dendritic spine density in rat hippocampal neurons. MAGI2 regulates AMPARs in a GluA2-dependent, activity-independent manner, and its overexpression hampers NMDA-induced AMPAR internalization and prevents LTD induction. RNAi knockdown, overexpression in hippocampal neurons, electrophysiology (AMPAR-mediated EPSC), dendritic spine imaging, surface AMPAR quantification The Journal of neuroscience High 22593065
2013 MiR-134/487b/655 cluster directly targets MAGI2 mRNA, suppressing MAGI2 expression to destabilize PTEN and promote TGF-β1-induced EMT and gefitinib resistance in lung adenocarcinoma cells. miRNA array, qRT-PCR, overexpression/knockdown of miRNAs, western blot for MAGI2 and PTEN, EMT markers, drug resistance assays Molecular cancer therapeutics Medium 24258346
2014 MAGI2 is required for kidney filtration barrier integrity and podocyte survival in mice; MAGI2-null mice develop progressive proteinuria, podocyte foot process effacement, loss of nephrin and dendrin at the slit diaphragm, nuclear translocation of dendrin, enhanced cathepsin L expression, and die of renal failure by 3 months. Homologous recombination knockout mice, immunohistochemistry, electron microscopy, proteinuria measurement Proceedings of the National Academy of Sciences of the United States of America High 25271328
2014 MAGI2-null mice show neonatal lethality with podocyte morphological abnormalities; loss of MAGI2 causes significant decreases in nephrin and dendrin at the slit diaphragm, nuclear translocation of dendrin, and enhanced cathepsin L expression, which is critical for rearrangement of the actin cytoskeleton in podocytes. Homozygous gene deletion mouse model, immunohistological analysis, ultrastructural analysis The American journal of pathology High 25108225
2014 Phosphorylation of the Usher syndrome protein SANS by CK2 at an internal PDZ-binding motif in its SAM domain regulates SANS-MAGI2 complex assembly; phosphorylated SANS tightly regulates MAGI2-mediated clathrin-dependent endocytosis, which in turn regulates ciliogenesis. USH1G patient mutations in SANS that eliminate MAGI2 binding deregulate endocytosis and disrupt photoreceptor cell function. Co-immunoprecipitation, RNAi depletion, kinase assay (CK2), endocytosis assays, localization in retinal photoreceptor cells, patient mutation analysis Human molecular genetics High 24608321
2015 MAGI2 is a transcriptional target of WT1 (Wilms' tumor suppressor) in podocytes, and is required for normal development of the embryonic zebrafish kidney as shown by functional assays in zebrafish. ChIP-seq, cDNA microarray, zebrafish functional assays (morpholino knockdown) Journal of the American Society of Nephrology Medium 25556170
2016 MAGI2 mutations (frameshift and duplication) cause congenital nephrotic syndrome by disrupting podocyte MAGI2 expression, consistent with its essential role in interacting with nephrin and regulating podocyte cytoskeleton and slit diaphragm dynamics. Whole-exome sequencing of patients, immunohistochemistry of patient kidney sections Journal of the American Society of Nephrology Medium 27932480
2019 MAGI2 forms a complex with the Rap1 guanine nucleotide exchange factor RapGEF2; co-expression of RapGEF2 with wild-type but not MAGI2 CNS variants enhances Rap1 GTPase activation. Podocyte-specific RapGEF2 deletion in mice causes glomerulosclerosis similar to MAGI2 knockout, and pharmacological Rap1 activation rescues actin cytoskeletal defects in human podocytes expressing MAGI2 CNS mutations. Co-immunoprecipitation, Rap1 activation assays, podocyte-specific conditional knockout mice, pharmacological rescue, patient kidney immunostaining Kidney international High 31171376

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2003 AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding. Cell 689 14505569
2000 Evidence for regulation of the PTEN tumor suppressor by a membrane-localized multi-PDZ domain containing scaffold protein MAGI-2. Proceedings of the National Academy of Sciences of the United States of America 340 10760291
2007 Structural and biochemical studies of ALIX/AIP1 and its role in retrovirus budding. Cell 272 17350572
1999 Cloning of AIP1, a novel protein that associates with the apoptosis-linked gene ALG-2 in a Ca2+-dependent reaction. The Journal of biological chemistry 201 9880530
2002 Oncogenic human papillomavirus E6 proteins target the MAGI-2 and MAGI-3 proteins for degradation. Oncogene 166 12140759
2013 MiR-134/487b/655 cluster regulates TGF-β-induced epithelial-mesenchymal transition and drug resistance to gefitinib by targeting MAGI2 in lung adenocarcinoma cells. Molecular cancer therapeutics 158 24258346
2003 Coordinated regulation of actin filament turnover by a high-molecular-weight Srv2/CAP complex, cofilin, profilin, and Aip1. Current biology : CB 149 14680631
2008 Actin disassembly by cofilin, coronin, and Aip1 occurs in bursts and is inhibited by barbed-end cappers. The Journal of cell biology 143 18663144
2003 YPXL/I is a protein interaction motif recognized by aspergillus PalA and its human homologue, AIP1/Alix. Molecular and cellular biology 137 12588984
2005 Cell junction-associated proteins IQGAP1, MAGI-2, CASK, spectrins, and alpha-actinin are components of the nephrin multiprotein complex. Proceedings of the National Academy of Sciences of the United States of America 136 15994232
2007 Associations with tight junction genes PARD3 and MAGI2 in Dutch patients point to a common barrier defect for coeliac disease and ulcerative colitis. Gut 129 17989107
2007 Potent rescue of human immunodeficiency virus type 1 late domain mutants by ALIX/AIP1 depends on its CHMP4 binding site. Journal of virology 127 17428861
2003 AIP1 mediates TNF-alpha-induced ASK1 activation by facilitating dissociation of ASK1 from its inhibitor 14-3-3. The Journal of clinical investigation 127 12813029
2001 Regulation of PTEN binding to MAGI-2 by two putative phosphorylation sites at threonine 382 and 383. Cancer research 127 11431330
2004 The actin-interacting protein AIP1 is essential for actin organization and plant development. Current biology : CB 124 14738737
2006 Rapid actin monomer-insensitive depolymerization of Listeria actin comet tails by cofilin, coronin, and Aip1. The Journal of cell biology 120 17060499
2007 Caspase-11 regulates cell migration by promoting Aip1-Cofilin-mediated actin depolymerization. Nature cell biology 114 17293856
2019 LncRNA MAGI2-AS3 Is Regulated by BRD4 and Promotes Gastric Cancer Progression via Maintaining ZEB1 Overexpression by Sponging miR-141/200a. Molecular therapy. Nucleic acids 113 31837602
2000 The glioma-associated protein SETA interacts with AIP1/Alix and ALG-2 and modulates apoptosis in astrocytes. The Journal of biological chemistry 111 10858458
1999 XAIP1: a Xenopus homologue of yeast actin interacting protein 1 (AIP1), which induces disassembly of actin filaments cooperatively with ADF/cofilin family proteins. Journal of cell science 109 10212149
2001 Yeast PalA/AIP1/Alix homolog Rim20p associates with a PEST-like region and is required for its proteolytic cleavage. Journal of bacteriology 107 11698381
2001 beta 1-adrenergic receptor association with the synaptic scaffolding protein membrane-associated guanylate kinase inverted-2 (MAGI-2). Differential regulation of receptor internalization by MAGI-2 and PSD-95. The Journal of biological chemistry 106 11526121
2006 Aip1 and cofilin promote rapid turnover of yeast actin patches and cables: a coordinated mechanism for severing and capping filaments. Molecular biology of the cell 103 16611742
2004 AIP1/DAB2IP, a novel member of the Ras-GAP family, transduces TRAF2-induced ASK1-JNK activation. The Journal of biological chemistry 102 15310755
2004 Alix/AIP1 antagonizes epidermal growth factor receptor downregulation by the Cbl-SETA/CIN85 complex. Molecular and cellular biology 101 15456872
2004 Vinculin controls PTEN protein level by maintaining the interaction of the adherens junction protein beta-catenin with the scaffolding protein MAGI-2. The Journal of biological chemistry 101 15579911
2008 AIP1 is critical in transducing IRE1-mediated endoplasmic reticulum stress response. The Journal of biological chemistry 98 18281285
2008 Infantile spasms is associated with deletion of the MAGI2 gene on chromosome 7q11.23-q21.11. American journal of human genetics 97 18565486
2007 Mutations in the cofilin partner Aip1/Wdr1 cause autoinflammatory disease and macrothrombocytopenia. Blood 95 17515402
2007 Human immunodeficiency virus type 1 Gag engages the Bro1 domain of ALIX/AIP1 through the nucleocapsid. Journal of virology 92 18032513
2006 Synaptic scaffolding molecule (S-SCAM) membrane-associated guanylate kinase with inverted organization (MAGI)-2 is associated with cell adhesion molecules at inhibitory synapses in rat hippocampal neurons. Journal of neurochemistry 81 17059560
2003 SETA/CIN85/Ruk and its binding partner AIP1 associate with diverse cytoskeletal elements, including FAKs, and modulate cell adhesion. Journal of cell science 80 12771190
2008 AIP1 functions as an endogenous inhibitor of VEGFR2-mediated signaling and inflammatory angiogenesis in mice. The Journal of clinical investigation 76 19033661
2007 RIP1-mediated AIP1 phosphorylation at a 14-3-3-binding site is critical for tumor necrosis factor-induced ASK1-JNK/p38 activation. The Journal of biological chemistry 76 17389591
2005 AIP1/Alix is a binding partner of Sendai virus C protein and facilitates virus budding. Journal of virology 75 15994787
2008 AIP1 recruits phosphatase PP2A to ASK1 in tumor necrosis factor-induced ASK1-JNK activation. Circulation research 74 18292600
2010 Loss of Aip1 reveals a role in maintaining the actin monomer pool and an in vivo oligomer assembly pathway. The Journal of cell biology 70 20231387
2020 Deregulated lncRNA MAGI2-AS3 in Alzheimer's disease attenuates amyloid-β induced neurotoxicity and neuroinflammation by sponging miR-374b-5p. Experimental gerontology 67 33279663
2018 Long non-coding RNA (lncRNA) MAGI2-AS3 inhibits breast cancer cell growth by targeting the Fas/FasL signalling pathway. Human cell 67 29679339
2014 Aip1 destabilizes cofilin-saturated actin filaments by severing and accelerating monomer dissociation from ends. Current biology : CB 66 25448002
2019 LncRNA MAGI2-AS3 inhibits hepatocellular carcinoma cell proliferation and migration by targeting the miR-374b-5p/SMG1 signaling pathway. Journal of cellular physiology 65 30924168
2006 Stargazin and other transmembrane AMPA receptor regulating proteins interact with synaptic scaffolding protein MAGI-2 in brain. The Journal of neuroscience : the official journal of the Society for Neuroscience 65 16870733
2006 The YLDL sequence within Sendai virus M protein is critical for budding of virus-like particles and interacts with Alix/AIP1 independently of C protein. Journal of virology 64 17166905
2004 The penta-EF-hand protein ALG-2 interacts with a region containing PxY repeats in Alix/AIP1, which is required for the subcellular punctate distribution of the amino-terminal truncation form of Alix/AIP1. Journal of biochemistry 62 14999017
1999 Cooperation of two actin-binding proteins, cofilin and Aip1, in Saccharomyces cerevisiae. Genes to cells : devoted to molecular & cellular mechanisms 60 10231390
2016 MAGI2 Mutations Cause Congenital Nephrotic Syndrome. Journal of the American Society of Nephrology : JASN 59 27932480
2004 Src phosphorylation of Alix/AIP1 modulates its interaction with binding partners and antagonizes its activities. The Journal of biological chemistry 59 15557335
2017 Functions of actin-interacting protein 1 (AIP1)/WD repeat protein 1 (WDR1) in actin filament dynamics and cytoskeletal regulation. Biochemical and biophysical research communications 58 29056508
2007 The flare gene, which encodes the AIP1 protein of Drosophila, functions to regulate F-actin disassembly in pupal epidermal cells. Genetics 58 17565945
2018 Long noncoding RNA MAGI2-AS3 regulates CCDC19 expression by sponging miR-15b-5p and suppresses bladder cancer progression. Biochemical and biophysical research communications 57 30442369
2015 Integration of Cistromic and Transcriptomic Analyses Identifies Nphs2, Mafb, and Magi2 as Wilms' Tumor 1 Target Genes in Podocyte Differentiation and Maintenance. Journal of the American Society of Nephrology : JASN 55 25556170
2014 Aip1 promotes actin filament severing by cofilin and regulates constriction of the cytokinetic contractile ring. The Journal of biological chemistry 54 25451933
2007 MAGI-2 Inhibits cell migration and proliferation via PTEN in human hepatocarcinoma cells. Archives of biochemistry and biophysics 53 17880912
2006 Interactions between Nef and AIP1 proliferate multivesicular bodies and facilitate egress of HIV-1. Retrovirology 52 16764724
2005 Functions of early (AP-2) and late (AIP1/ALIX) endocytic proteins in equine infectious anemia virus budding. The Journal of biological chemistry 52 16215227
2016 MiR-487a Promotes TGF-β1-induced EMT, the Migration and Invasion of Breast Cancer Cells by Directly Targeting MAGI2. International journal of biological sciences 48 27019625
2012 S-SCAM/MAGI-2 is an essential synaptic scaffolding molecule for the GluA2-containing maintenance pool of AMPA receptors. The Journal of neuroscience : the official journal of the Society for Neuroscience 48 22593065
2011 AIP1 prevents graft arteriosclerosis by inhibiting interferon-γ-dependent smooth muscle cell proliferation and intimal expansion. Circulation research 48 21700930
2005 AIP1/WDR1 supports mitotic cell rounding. Biochemical and biophysical research communications 47 15629458
2019 Long non-coding RNA MAGI2-AS3 inhibits breast cancer cell migration and invasion via sponging microRNA-374a. Cancer biomarkers : section A of Disease markers 44 30883342
2013 AIP1 suppresses atherosclerosis by limiting hyperlipidemia-induced inflammation and vascular endothelial dysfunction. Arteriosclerosis, thrombosis, and vascular biology 44 23413429
2020 MAGI2-AS3 inhibits breast cancer by downregulating DNA methylation of MAGI2. Journal of cellular physiology 42 32730644
2019 lncRNA MAGI2-AS3 Prevents the Development of HCC via Recruiting KDM1A and Promoting H3K4me2 Demethylation of the RACGAP1 Promoter. Molecular therapy. Nucleic acids 42 31629962
2007 Recruitment of Alix/AIP1 to the plasma membrane by Sendai virus C protein facilitates budding of virus-like particles. Virology 42 18028977
2019 Long noncoding MAGI2-AS3 promotes colorectal cancer progression through regulating miR-3163/TMEM106B axis. Journal of cellular physiology 41 31709544
2009 MAGI2 genetic variation and inflammatory bowel disease. Inflammatory bowel diseases 40 18720471
2014 MAGI-2 scaffold protein is critical for kidney barrier function. Proceedings of the National Academy of Sciences of the United States of America 37 25271328
2020 LncRNA MAGI2-AS3 inhibits bladder cancer progression by targeting the miR-31-5p/TNS1 axis. Aging 35 33231563
2009 An order of magnitude faster AIP1-associated actin disruption than nucleation by the Arp2/3 complex in lamellipodia. PloS one 35 19290054
2022 Long noncoding RNA MAGI2-AS3 regulates the H2O2 level and cell senescence via HSPA8. Redox biology 33 35797800
2014 MAGI-2 is critical for the formation and maintenance of the glomerular filtration barrier in mouse kidney. The American journal of pathology 33 25108225
2012 Both internalization and AIP1 association are required for tumor necrosis factor receptor 2-mediated JNK signaling. Arteriosclerosis, thrombosis, and vascular biology 33 22743059
2019 Long Non-Coding RNA MAGI2-AS3 is a New Player with a Tumor Suppressive Role in High Grade Serous Ovarian Carcinoma. Cancers 32 31842477
2023 MAGI2-AS3 and miR-374b-5p as Putative Regulators of Multiple Sclerosis via Modulating the PTEN/AKT/IRF-3/IFN-β Axis: New Clinical Insights. ACS chemical neuroscience 31 36878000
2021 Roles of lncRNA MAGI2-AS3 in human cancers. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 31 34126355
2012 Common variants in MAGI2 gene are associated with increased risk for cognitive impairment in schizophrenic patients. PloS one 31 22649501
2020 LncRNA MAGI2-AS3 Overexpression Sensitizes Esophageal Cancer Cells to Irradiation Through Down-Regulation of HOXB7 via EZH2. Frontiers in cell and developmental biology 30 33330444
2015 AIP1-mediated actin disassembly is required for postnatal germ cell migration and spermatogonial stem cell niche establishment. Cell death & disease 29 26181199
2009 AIP1 functions as Arf6-GAP to negatively regulate TLR4 signaling. The Journal of biological chemistry 29 19948740
2004 Ca2+ binding to EF hands 1 and 3 is essential for the interaction of apoptosis-linked gene-2 with Alix/AIP1 in ocular melanoma. Biochemistry 29 15366927
2003 PKC regulates the delta2 glutamate receptor interaction with S-SCAM/MAGI-2 protein. Biochemical and biophysical research communications 29 12589829
2020 LncRNA MAGI2-AS3 inhibits the self-renewal of leukaemic stem cells by promoting TET2-dependent DNA demethylation of the LRIG1 promoter in acute myeloid leukaemia. RNA biology 28 32174258
2019 LncRNA MAGI2-AS3 suppresses the proliferation and invasion of non-small cell lung carcinoma through miRNA-23a-3p/PTEN axis. European review for medical and pharmacological sciences 28 31539127
2014 AIP1 mediates vascular endothelial cell growth factor receptor-3-dependent angiogenic and lymphangiogenic responses. Arteriosclerosis, thrombosis, and vascular biology 27 24407031
2014 Arabidopsis AIP1-2 restricted by WER-mediated patterning modulates planar polarity. Development (Cambridge, England) 27 25428588
2022 AIP1 suppresses neovascularization by inhibiting the NOX4-induced NLRP3/NLRP6 imbalance in a murine corneal alkali burn model. Cell communication and signaling : CCS 26 35524333
2020 Long noncoding RNA MAGI2-AS3/miR-218-5p/GDPD5/SEC61A1 axis drives cellular proliferation and migration and confers cisplatin resistance in nasopharyngeal carcinoma. International forum of allergy & rhinology 26 32450008
2015 MAGI2/S-SCAM outside brain. Journal of biochemistry 26 25637633
2020 MAGI2-AS3 suppresses MYC signaling to inhibit cell proliferation and migration in ovarian cancer through targeting miR-525-5p/MXD1 axis. Cancer medicine 25 32681706
2010 Genetic evidence for concerted control of actin dynamics in cytokinesis, endocytic traffic, and cell motility by coronin and Aip1. Cytoskeleton (Hoboken, N.J.) 25 20506401
2007 Actin organization and root hair development are disrupted by ethanol-induced overexpression of Arabidopsis actin interacting protein 1 (AIP1). The New phytologist 25 17335497
2020 Long Noncoding MAGI2-AS3 Suppresses Several Cellular Processes of Lung Squamous Cell Carcinoma Cells by Regulating miR-374a/b-5p/CADM2 Axis. Cancer management and research 24 32021443
2014 Phosphorylation of the Usher syndrome 1G protein SANS controls Magi2-mediated endocytosis. Human molecular genetics 23 24608321
2010 Deletion of 7q11.21-q11.23 and infantile spasms without deletion of MAGI2. American journal of medical genetics. Part A 23 20101691
2007 Regulation of HTLV-1 Gag budding by Vps4A, Vps4B, and AIP1/Alix. Virology journal 23 17601348
2003 AIP1: a new player in TNF signaling. The Journal of clinical investigation 23 12813014
2021 LncRNA MAGI2-AS3 inhibits tumor progression and angiogenesis by regulating ACY1 via interacting with transcription factor HEY1 in clear cell renal cell carcinoma. Cancer gene therapy 22 34002044
2020 Catastrophic actin filament bursting by cofilin, Aip1, and coronin. The Journal of biological chemistry 22 32723865
2019 Disruption of MAGI2-RapGEF2-Rap1 signaling contributes to podocyte dysfunction in congenital nephrotic syndrome caused by mutations in MAGI2. Kidney international 22 31171376
2021 Novel insights for lncRNA MAGI2-AS3 in solid tumors. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 21 33761624