Affinage

LSM1

U6 snRNA-associated Sm-like protein LSm1 · UniProt O15116

Length
133 aa
Mass
15.2 kDa
Annotated
2026-04-28
57 papers in source corpus 25 papers cited in narrative 25 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

LSM1 is the defining subunit of the cytoplasmic Lsm1-7-Pat1 hetero-octameric ring complex that functions as a central activator of mRNA decapping in the 5′-to-3′ decay pathway and as a selective translational repressor. The Lsm1-7 heptamer adopts a 1-2-3-6-5-7-4 ring topology in which the Lsm1 C-terminal extension plugs the RNA exit channel, gating 3′-end access; cooperatively with Pat1, the complex preferentially binds oligoadenylated and oligouridylated 3′ tails of deadenylated mRNAs, protects 3′ ends from trimming, and facilitates post-binding steps required for decapping (PMID:24139796, PMID:18719247, PMID:19643916, PMID:25035297, PMID:32518066). Beyond general mRNA turnover, Lsm1-7-Pat1 controls histone mRNA levels to maintain genomic stability, mediates decay of pericentromeric satellite RNA to regulate histone H3.3 variant incorporation in zygotes, represses translation initiation of stress-induced mRNAs, and directly engages viral RNA genomes to modulate viral replication and translation (PMID:21487390, PMID:36810573, PMID:30059503, PMID:20181739, PMID:26092942). The complex localizes to cytoplasmic processing bodies (P-bodies) together with Dcp1/2 and Xrn1, and Pat1-driven multimerization of Lsm1-7 promotes liquid–liquid phase separation with decapping factors (PMID:12515382, PMID:32513655).

Mechanistic history

Synthesis pass · year-by-year structured walk · 17 steps
  1. 1997 Medium

    Identification of LSM1 (CaSm) as an Sm-domain protein whose expression supports the transformed phenotype of cancer cells established it as a gene of functional interest, though no molecular mechanism was yet defined.

    Evidence Antisense RNA knockdown reduced anchorage-independent growth in pancreatic cancer cells

    PMID:9230209

    Open questions at the time
    • No molecular mechanism linking LSM1 to transformation was identified
    • No binding partners or RNA substrates characterized
  2. 2002 High

    Demonstrating that hLSm1-7 form a cytoplasmic heptameric complex enriched in P-bodies with decapping enzymes Dcp1/2 and Xrn1 established the cellular context for LSM1 function in mRNA decay, distinguishing it from the nuclear Lsm2-8 complex.

    Evidence Immunofluorescence, FRET, and co-expression of wild-type and mutant LSm proteins in human cells

    PMID:12515382

    Open questions at the time
    • Direct RNA substrates not identified
    • Mechanism of decapping activation unknown
  3. 2005 High

    Systematic mutagenesis revealed that both the Sm domain and the C-terminal domain of Lsm1 are required for mRNA decay and that the complex protects mRNA 3′ ends, separating RNA-binding from P-body localization functions.

    Evidence Site-directed mutagenesis with mRNA decay and 3′-end protection assays in S. cerevisiae

    PMID:15716506

    Open questions at the time
    • Structural basis for C-terminal domain function unknown
    • Molecular mechanism of 3′-end protection unresolved
  4. 2008 High

    Establishing that mutations abolishing preferential binding to oligoadenylated RNA cause severe mRNA decay defects in vivo demonstrated that oligo(A)-tail recognition is the functional basis for substrate selectivity of the Lsm1-7-Pat1 complex.

    Evidence In vitro RNA binding with purified mutant complexes correlated with in vivo decay phenotypes in yeast

    PMID:18719247

    Open questions at the time
    • How oligouridylated substrates are recognized was unresolved
    • Contribution of Pat1 to RNA binding not yet dissected
  5. 2009 High

    Dissection of binding versus post-binding steps showed that RNA binding alone suffices for 3′-end protection but that decapping activation requires additional conformational or recruitment events, establishing a two-step model for Lsm1-7-Pat1 function.

    Evidence Multiple lsm1 point mutants with dominant-negative analysis, mRNA decay and 3′-end protection assays in yeast

    PMID:19643916

    Open questions at the time
    • Nature of the post-binding step (conformational change, factor recruitment) not identified
    • No structural snapshot of the post-binding intermediate
  6. 2009 High

    Showing that Lsm1-7-Pat1 recognizes U-tracts in addition to oligo(A) tails expanded the substrate repertoire to include oligouridylated histone mRNAs and demonstrated a shared decay mechanism for distinct 3′-end modifications.

    Evidence In vitro RNA binding and in vivo mRNA decay measurements in yeast

    PMID:19279404

    Open questions at the time
    • Structural basis for U-tract recognition not yet resolved at atomic level
  7. 2009 Medium

    Overexpression of LSM1 was shown to deplete U6 snRNA by sequestering shared Lsm2-7 subunits from the nuclear Lsm2-8 complex, revealing competition between cytoplasmic and nuclear Lsm complexes as a regulatory constraint.

    Evidence Genetic and RNA quantification assays in S. cerevisiae

    PMID:19596813

    Open questions at the time
    • Physiological relevance of Lsm subunit competition under normal expression levels not demonstrated
    • Single laboratory study
  8. 2010 High

    Direct binding of reconstituted Lsm1-7 to specific viral RNA elements (BMV tRNA-like structure and internal A-rich regions) demonstrated that the complex engages viral genomes to regulate translation and replication.

    Evidence In vitro reconstitution of recombinant Lsm1-7 with RNA binding assays plus in vivo BMV replication analysis in yeast

    PMID:20181739

    Open questions at the time
    • Mechanism by which Lsm1-7 promotes viral replication versus translation not separated
  9. 2011 High

    Establishing that Lsm1 controls histone mRNA decay and that loss of Lsm1 causes excess histone accumulation leading to replication-fork instability linked mRNA turnover to genome integrity maintenance.

    Evidence Genetic epistasis (histone gene dosage suppression), mRNA decay assays, and DNA damage sensitivity in S. cerevisiae

    PMID:21487390

    Open questions at the time
    • Whether this pathway operates in mammalian cells not tested
    • Whether other decapping activators contribute was not assessed
  10. 2012 High

    Deletion and trans-complementation of the Lsm1 C-terminal domain demonstrated it cooperates with the Sm domain for RNA binding, resolving its functional contribution at the biochemical level.

    Evidence Purified complex in vitro RNA binding, in vivo decay and 3′-end protection assays, trans-complementation in yeast

    PMID:22450758

    Open questions at the time
    • Atomic-resolution structure of the C-terminal domain bound to RNA not yet available
  11. 2013 High

    Crystal structures of Lsm1-7 alone and bound to Pat1C revealed the 1-2-3-6-5-7-4 ring topology with the Lsm1 C-terminal extension plugging the central pore, and showed Pat1 contacts Lsm2-3, not Lsm1, providing the first structural framework for the complex.

    Evidence X-ray crystallography at 2.3 Å (Lsm1-7) and 3.7 Å (Lsm1-7-Pat1C) in S. cerevisiae

    PMID:24139796 PMID:24141094

    Open questions at the time
    • No RNA-bound structure of the full octameric complex
    • How the pore plug is relieved for RNA access not resolved
  12. 2014 High

    Reconstitution showing that neither Lsm1-7 alone nor Pat1 alone has appreciable RNA-binding or oligo(A)-discriminating activity established that the full Lsm1-7-Pat1 complex is the functional unit and that Pat1 directly contacts RNA.

    Evidence Purified component reconstitution with in vitro RNA binding assays and in vivo genetic validation

    PMID:25035297

    Open questions at the time
    • Structural basis for Pat1-RNA contact not resolved
    • Stoichiometry of Pat1 in the RNA-bound complex unclear
  13. 2015 High

    Separation of Lsm1-7-Pat1 roles in BMV RNA translation versus replication-complex recruitment showed that intrinsic RNA binding is required only for translation, while a direct protein–protein interaction between viral 1a and the complex mediates replication recruitment.

    Evidence Epistatic analysis with lsm1 alleles, co-IP, and RNA-binding assays in yeast BMV system

    PMID:26092942

    Open questions at the time
    • Interface between BMV 1a and the Lsm1-7-Pat1 complex not structurally characterized
  14. 2018 High

    Discovering that Lsm1-7-Pat1 preferentially binds and translationally represses stress-induced mRNAs expanded its function beyond mRNA decay to translational regulation, particularly at initiation.

    Evidence MS2 RNA pulldown, polysome profiling, and 5P-Seq in yeast under osmotic stress

    PMID:30059503

    Open questions at the time
    • Mechanism of translational repression (competition with eIF4F, ribosome stalling?) not defined
    • Whether translational repression is independent of decapping not fully resolved
  15. 2020 High

    High-resolution structures of RNA-bound Lsm1-7 established that the complex loads from the 3′ end, discriminates against cyclic phosphates, recognizes terminal purines via Lsm5, and that the Lsm1 C-terminal region gates internal scanning along RNA.

    Evidence Cryo-EM and X-ray crystallography of Lsm1-7–RNA complexes with mutagenesis

    PMID:32518066

    Open questions at the time
    • Full octameric Lsm1-7-Pat1–RNA structure not yet obtained
    • Functional relevance of internal scanning in vivo not demonstrated
  16. 2020 High

    Pat1 was shown to broaden RNA specificity, enhance binding cooperativity, promote Lsm1-7 multimerization, and drive liquid–liquid phase separation with Dcp1/Dcp2, linking the biochemistry of RNA recognition to P-body assembly.

    Evidence In vitro reconstitution with purified proteins, RNA binding, multimerization, and phase separation assays

    PMID:32513655

    Open questions at the time
    • In vivo relevance of Pat1-driven LLPS for decapping kinetics not tested
    • Stoichiometry of Lsm1-7 multimers in phase-separated droplets unknown
  17. 2023 High

    Lsm1-mediated decay of pericentromeric major satellite RNA was shown to be required for asymmetric histone H3.3 incorporation in the male pronucleus, linking RNA turnover to epigenetic reprogramming in early zygotic development.

    Evidence siRNA knockdown in mouse zygotes with histone ChIP, RNA quantification, and rescue by MajSat RNA knockdown

    PMID:36810573

    Open questions at the time
    • Whether Lsm1-7-Pat1 or Lsm1-7 alone mediates MajSat decay in zygotes not determined
    • Generality to other repeat-derived RNAs not tested

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structure of the full RNA-bound Lsm1-7-Pat1 octamer, the molecular mechanism of the post-binding decapping-activation step, how translational repression is mechanistically executed independently of decay, and the physiological regulation of Lsm1 expression levels that balance cytoplasmic and nuclear Lsm complex pools.
  • No full octameric RNA-bound structure
  • Post-binding decapping activation step molecularly undefined
  • Translational repression mechanism versus decay not separated at the structural level

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 6 GO:0098772 molecular function regulator activity 2
Localization
GO:0005829 cytosol 2 GO:0031410 cytoplasmic vesicle 2
Pathway
R-HSA-8953854 Metabolism of RNA 8 R-HSA-1640170 Cell Cycle 1 R-HSA-392499 Metabolism of proteins 1
Partners
LSM2LSM3LSM4LSM5LSM6LSM7PAT1
Complex memberships
Lsm1-7 heptameric ringLsm1-7-Pat1 octameric complex

Evidence

Reading pass · 25 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2002 Human LSm1-7 proteins colocalize with mRNA-degrading enzymes Dcp1/2 and Xrn1 in discrete cytoplasmic foci (P-bodies); FRET and co-expression studies show hLSm1-7 form a heptameric complex similar to yeast, and complex formation is required for enrichment in these foci. hLSm1 is predominantly cytoplasmic, whereas hLSm8 is not found in the same foci. Subcellular localization by immunofluorescence, FRET, co-expression of wild-type and mutant LSm proteins RNA (New York, N.Y.) High 12515382
2013 Crystal structure of S. cerevisiae Lsm1-7 at 2.3 Å resolution reveals a heptameric ring with Lsm1-2-3-6-5-7-4 topology; the C-terminal extension of Lsm1 plugs the exit site of the central channel and approaches RNA-binding pockets. Structure of Lsm1-7 bound to Pat1 C-terminal domain at 3.7 Å shows Pat1 is recognized by Lsm2 and Lsm3, not Lsm1. X-ray crystallography (2.3 Å and 3.7 Å resolution structures) Cell reports High 24139796
2005 Mutations in yeast Lsm1 affecting predicted RNA-binding and inter-subunit interaction residues impair mRNA decay; mutations affecting RNA contact residues do not affect P-body localization; the C-terminal domain of Lsm1 is important for mRNA decay function in addition to the Sm domain. mRNA 3'-end protection requires binding of the Lsm1-7-Pat1 complex to mRNA prior to decapping activation. Site-directed mutagenesis, mRNA decay assays, genetic phenotypic analysis in S. cerevisiae Genetics High 15716506
2009 The Lsm1-7-Pat1 complex has a strong intrinsic binding preference for oligoadenylated mRNAs over polyadenylated mRNAs, and this preferential binding is crucial for its mRNA decay function; the complex can also recognize U-tracts at the 3' end of RNA to facilitate decapping and 5'-to-3' decay of histone mRNAs in response to oligouridylation. In vitro RNA binding assays, genetic analysis, mRNA decay measurements in yeast RNA biology High 19279404
2013 Lsm2 and Lsm3 bridge the interaction between the C-terminus of Pat1 and the Lsm1-7 complex; the crystal structure of Lsm2-3-Pat1C shows three Pat1C molecules surrounding a heptameric ring formed by Lsm2-3; the Lsm2-3-Pat1C complex stimulates decapping in vitro similarly to the full Lsm1-7-Pat1C complex; structure-based mutagenesis confirmed the importance of Lsm2-3-Pat1C interactions for decapping activation in vivo. X-ray crystallography, in vitro decapping assay, structure-based mutagenesis, in vivo decapping assay Cell research High 24247251
2014 Pat1 contributes directly to RNA binding of the Lsm1-7-Pat1 complex; Lsm1-7 complex alone and Pat1 fragments alone have very low RNA binding activity and cannot discriminate oligoadenylated RNA, but reconstitution of the complex restores RNA binding and oligo(A) preference; Pat1 directly contacts RNA in the context of the complex. The middle domain of Pat1 is essential for its interaction with the Lsm1-7 complex in vivo. Protein purification, in vitro RNA binding assays, complex reconstitution, genetic interaction studies RNA (New York, N.Y.) High 25035297
2009 Decapping by the Lsm1-7-Pat1 complex requires both binding of the complex to the mRNA and facilitation of post-binding events; RNA binding per se is sufficient for 3'-end protection; lsm1 mutants (lsm1-9, lsm1-14) that retain partial RNA binding but block post-binding steps show dominant inhibition of mRNA decay when overproduced, while lsm1-8 (nearly abolishes RNA binding) does not. Genetic analysis with multiple lsm1 point mutants, mRNA decay assays, 3'-end protection assays, overexpression dominant-negative analysis in S. cerevisiae RNA (New York, N.Y.) High 19643916
2008 lsm1 mutations that abolish preferential binding to oligoadenylated RNA in vitro (while retaining complex integrity and binding to U-tract RNAs) cause a strong mRNA decay defect in vivo, demonstrating that the oligo(A) tail-mediated enhancement of Lsm1p-7p-Pat1p complex–mRNA interaction is crucial for mRNA decay. In vitro RNA binding assays with purified mutant complexes, mRNA decay analysis in vivo in S. cerevisiae RNA (New York, N.Y.) High 18719247
2012 The C-terminal domain (CTD) of Lsm1, in addition to the Sm domain, is required for normal RNA-binding activity of the Lsm1-7-Pat1 complex; deletion of the CTD (while preserving the Sm domain) severely impairs in vitro RNA binding and causes mRNA decay and 3'-end protection defects in vivo; overexpression of the CTD polypeptide in trans partially suppresses these defects. Deletion mutagenesis, in vitro RNA binding assays with purified complexes, in vivo mRNA decay and 3'-end protection assays, trans-complementation RNA (New York, N.Y.) High 22450758
2016 Mutagenic analysis of the C-terminal extension of Lsm1 identified specific residues at the very C-terminal end that are functionally important for RNA binding and mRNA decay function of the Lsm1-7-Pat1 complex. Site-directed mutagenesis, in vitro RNA binding assays, in vivo mRNA decay assays in yeast PloS one Medium 27434131
2020 High-resolution cryo-EM/crystal structures of Lsm1-7 bound to RNA show the complex strongly discriminates against cyclic phosphates and tightly binds oligouridylate tracts with terminal purines; Lsm5 uniquely recognizes purine bases; Lsm1-7 loads onto RNA from the 3' end, and removal of the Lsm1 carboxy-terminal region allows the complex to scan along RNA, suggesting a gated mechanism for accessing internal binding sites. High-resolution cryo-EM and X-ray crystallography of Lsm complexes bound to RNA, mutagenesis RNA (New York, N.Y.) High 32518066
2010 Reconstituted recombinant LSm1-7 complexes directly bind to two distinct RNA target sequences in the BMV genome: a tRNA-like structure at the 3'-UTR and two internal A-rich single-stranded regions; these sequences regulate translation and replication of the BMV genome in vivo. In vitro reconstitution of recombinant LSm1-7, RNA binding assays, in vivo viral replication analysis RNA (New York, N.Y.) High 20181739
2015 The Lsm1-7-Pat1 complex acts differentially in viral RNA translation versus recruitment to replication: complex integrity is essential for both, but intrinsic RNA-binding ability is only required for translation. The BMV 1a protein interacts with Lsm1-7-Pat1 complex in an RNA-independent manner to mediate viral RNA recruitment to replication complexes. Genetic analysis with lsm1 mutant alleles in yeast BMV replication system, co-immunoprecipitation, RNA-binding assays RNA (New York, N.Y.) High 26092942
2011 Lsm1 promotes genomic stability by controlling histone mRNA decay in yeast; cells lacking Lsm1 accumulate excess histones, leading to replication-fork instability; reducing histone gene dosage suppresses the replication sensitivity of lsm1Δ cells, placing excess histone accumulation as the causative factor. Genetic epistasis (histone gene dosage suppression), mRNA decay assays, DNA damage sensitivity assays in S. cerevisiae The EMBO journal High 21487390
2009 In neuronal dendrites, LSm1 associates with intact mRNAs (not degradation intermediates) in a complex containing the cap-binding protein CBP80, suggesting the complex is assembled in the nucleus and transported to dendrites; neuronal LSm1 is partially nuclear and inhibition of mRNA synthesis increases its nuclear localization; LSm1 and CBP80 shift into dendritic spines upon glutamatergic receptor stimulation, indicating these mRNPs contribute to regulated local protein synthesis. Immunofluorescence localization, co-immunoprecipitation, live-cell imaging, pharmacological inhibition of transcription The Journal of cell biology Medium 19188494
2015 LSm1 binds to the 3' UTR of Dengue virus RNA; LSm1 knockdown by siRNA reduces viral RNA levels and infectious particle production; the LSm1-viral RNA interaction localizes to P-bodies in the cytoplasm. RNA pulldown/co-IP, siRNA knockdown, confocal immunofluorescence, RT-qPCR for viral RNA levels International journal of molecular medicine Medium 25872476
2013 The P-body protein LSm1 contributes to activation of HCV IRES-driven translation by miR-122 but is not required for miR-122-mediated repressive function at 3' UTR sites, miR-122 cleavage activity, or miR-122 stimulation of HCV replication; LSm1 does not influence RISC recruitment to the HCV 5' UTR, implying it acts downstream of target binding. siRNA knockdown of LSm1, HCV IRES reporter assays, replication assays, RISC recruitment assays Nucleic acids research Medium 24141094
2017 Pat1b forms a nuclear complex with the Lsm2-8 heptamer that binds U6 snRNA in Cajal bodies; co-IP and immunofluorescence demonstrate Pat1b/Lsm2-8/U6 snRNA/SART3 interactions connecting to tri-snRNP components; this is distinct from the cytoplasmic Pat1b/Lsm1-7 decapping complex, demonstrating dual roles for Pat1b via distinct Lsm complexes. Co-immunoprecipitation, immunofluorescence, RNAi, RNA sequencing Cell reports Medium 28768202
2018 The Lsm1-7/Pat1 complex preferentially binds stress-activated mRNAs and acts as a translational repressor in addition to its role in mRNA decay; lsm1 mutants show abnormally high association of mRNAs with polysomes under osmotic stress, and 5P-Seq reveals increased ribosome accumulation upstream of start codons, indicating the complex represses translation initiation particularly for highly expressed stress-induced mRNAs. MS2 RNA tagging/purification, polysome profiling, 5P-Seq (co-translational decay sequencing), genetic analysis in yeast PLoS genetics High 30059503
2020 Pat1 broadens RNA specificity of Lsm1-7 by enhancing binding to A-rich RNAs and increases cooperativity on all oligonucleotides tested; Pat1 promotes multimerization of the Lsm1-7 complex potentiated by RNA binding; Pat1's inherent ability to multimerize drives liquid-liquid phase separation with multivalent decapping enzyme complexes Dcp1/Dcp2. In vitro RNA binding assays with recombinant purified proteins, biochemical multimerization assays, phase separation assays RNA (New York, N.Y.) High 32513655
2011 Structure of the LSm657 assembly intermediate at 2.5 Å reveals three monomers forming a hexameric LSm657-657 ring with canonical Sm fold; NMR and pulldown studies show LSm657 can incorporate LSm23 to assemble further toward native LSm1-7 and LSm2-8 rings, identifying LSm657 as a functional assembly intermediate. X-ray crystallography (2.5 Å), NMR spectroscopy, pulldown assays Journal of molecular biology High 22001694
2009 LSM1 overexpression in yeast inhibits growth primarily by depleting U6 snRNA levels; excess Lsm1 reduces the availability of Lsm2-7 proteins that normally assemble with Lsm8 to form the Lsm2-8 complex that stabilizes U6 snRNA, thereby disrupting pre-mRNA splicing. Genetic analysis, U6 snRNA quantification, hypersensitivity assays in S. cerevisiae Nucleic acids research Medium 19596813
2023 LSM1-mediated decay of major satellite repeat RNA (MajSat RNA) is required for preferential incorporation of histone variant H3.3 into the male pronucleus; Lsm1 knockdown in mouse zygotes disrupts nonequilibrium histone H3.3 incorporation and asymmetric H3K9me3 modification; accumulated MajSat RNA in Lsm1-depleted oocytes causes abnormal H3.1 incorporation into the male pronucleus; knockdown of MajSat RNA reverses these defects. siRNA knockdown of Lsm1 in mouse zygotes, histone ChIP, RNA quantification, rescue experiments by MajSat RNA knockdown Nature communications High 36810573
2015 In C. elegans, lsm-1 mutants have impaired Insulin/IGF-1 signaling (IIS); heat stress-induced translocation of the FOXO transcription factor DAF-16 to the nucleus is dependent on lsm-1; lsm-1 mutants show heightened sensitivity to thermal stress and starvation while lsm-1 overexpression has the opposite effect; under stress, cytoplasmic LSm proteins aggregate into granules in an LSM-1-dependent manner. Genetic analysis (RNAi and mutants), DAF-16::GFP reporter, RNA-seq, stress assays in C. elegans RNA (New York, N.Y.) Medium 26150554
1997 CaSm (LSM1) encodes a 133-amino acid protein containing two Sm motifs; antisense CaSm RNA reduces anchorage-independent growth of pancreatic cancer cells, indicating CaSm expression is necessary for maintenance of the transformed state. Antisense RNA expression, soft agar colony formation assay Cancer research Medium 9230209

Source papers

Stage 0 corpus · 57 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 The human LSm1-7 proteins colocalize with the mRNA-degrading enzymes Dcp1/2 and Xrnl in distinct cytoplasmic foci. RNA (New York, N.Y.) 303 12515382
2022 Many roads lead to CASM: Diverse stimuli of noncanonical autophagy share a unifying molecular mechanism. Science advances 117 36288315
2013 Architecture of the Lsm1-7-Pat1 complex: a conserved assembly in eukaryotic mRNA turnover. Cell reports 65 24139796
2005 Mutations in the Saccharomyces cerevisiae LSM1 gene that affect mRNA decapping and 3' end protection. Genetics 54 15716506
2009 Lsm1-7-Pat1 complex: a link between 3' and 5'-ends in mRNA decay? RNA biology 52 19279404
2006 Transforming function of the LSM1 oncogene in human breast cancers with the 8p11-12 amplicon. Oncogene 51 17001308
2016 The LSM1-7 Complex Differentially Regulates Arabidopsis Tolerance to Abiotic Stress Conditions by Promoting Selective mRNA Decapping. The Plant cell 47 26764377
2011 Lsm1 promotes genomic stability by controlling histone mRNA decay. The EMBO journal 47 21487390
2009 Dendritic LSm1/CBP80-mRNPs mark the early steps of transport commitment and translational control. The Journal of cell biology 45 19188494
2025 A STING-CASM-GABARAP pathway activates LRRK2 at lysosomes. The Journal of cell biology 37 39812709
1997 CaSm: an Sm-like protein that contributes to the transformed state in cancer cells. Cancer research 37 9230209
2013 Lsm2 and Lsm3 bridge the interaction of the Lsm1-7 complex with Pat1 for decapping activation. Cell research 35 24247251
2014 Pat1 contributes to the RNA binding activity of the Lsm1-7-Pat1 complex. RNA (New York, N.Y.) 34 25035297
2010 LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication. RNA (New York, N.Y.) 34 20181739
2020 Molecular basis for the distinct cellular functions of the Lsm1-7 and Lsm2-8 complexes. RNA (New York, N.Y.) 31 32518066
2016 The CaSm (LSm1) oncogene promotes transformation, chemoresistance and metastasis of pancreatic cancer cells. Oncogenesis 31 26751936
2009 Activation of decapping involves binding of the mRNA and facilitation of the post-binding steps by the Lsm1-7-Pat1 complex. RNA (New York, N.Y.) 30 19643916
2017 Dual RNA Processing Roles of Pat1b via Cytoplasmic Lsm1-7 and Nuclear Lsm2-8 Complexes. Cell reports 28 28768202
2018 The Lsm1-7/Pat1 complex binds to stress-activated mRNAs and modulates the response to hyperosmotic shock. PLoS genetics 26 30059503
2008 CaSm (LSm-1) overexpression in lung cancer and mesothelioma is required for transformed phenotypes. American journal of respiratory cell and molecular biology 25 18218995
2015 The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms. RNA (New York, N.Y.) 23 26092942
2009 LSM1 over-expression in Saccharomyces cerevisiae depletes U6 snRNA levels. Nucleic acids research 23 19596813
2008 lsm1 mutations impairing the ability of the Lsm1p-7p-Pat1p complex to preferentially bind to oligoadenylated RNA affect mRNA decay in vivo. RNA (New York, N.Y.) 22 18719247
2013 The P body protein LSm1 contributes to stimulation of hepatitis C virus translation, but not replication, by microRNA-122. Nucleic acids research 20 24141094
2022 Integrated analysis of pivotal biomarker of LSM1, immune cell infiltration and therapeutic drugs in breast cancer. Journal of cellular and molecular medicine 18 35692083
2023 The role of LSM1 in breast cancer: Shaping metabolism and tumor-associated macrophage infiltration. Pharmacological research 17 37995895
2015 LSm1 binds to the Dengue virus RNA 3' UTR and is a positive regulator of Dengue virus replication. International journal of molecular medicine 17 25872476
2003 CaSm antisense gene therapy: a novel approach for the treatment of pancreatic cancer. Anticancer research 17 12894573
2002 Down-regulation of Lsm1 is involved in human prostate cancer progression. British journal of cancer 17 11953827
2015 Cytoplasmic LSM-1 protein regulates stress responses through the insulin/IGF-1 signaling pathway in Caenorhabditis elegans. RNA (New York, N.Y.) 15 26150554
2012 Both Sm-domain and C-terminal extension of Lsm1 are important for the RNA-binding activity of the Lsm1-7-Pat1 complex. RNA (New York, N.Y.) 15 22450758
2011 Structure of the LSm657 complex: an assembly intermediate of the LSm1-7 and LSm2-8 rings. Journal of molecular biology 15 22001694
2001 CaSm/gemcitabine chemo-gene therapy leads to prolonged survival in a murine model of pancreatic cancer. Surgery 15 11490361
2022 Arabidopsis RNA processing body components LSM1 and DCP5 aid in the evasion of translational repression during Cauliflower mosaic virus infection. The Plant cell 14 35511183
2020 Pdc2/Pat1 increases the range of decay factors and RNA bound by the Lsm1-7 complex. RNA (New York, N.Y.) 14 32513655
2021 Genomic Mapping of Splicing-Related Genes Identify Amplifications in LSM1, CLNS1A, and ILF2 in Luminal Breast Cancer. Cancers 11 34439272
2023 LSM1-mediated Major Satellite RNA decay is required for nonequilibrium histone H3.3 incorporation into parental pronuclei. Nature communications 10 36810573
2021 A new flavor of cellular Atg8-family protein lipidation - alternative conjugation to phosphatidylserine during CASM. Autophagy 10 34251968
2005 Establishing a murine pancreatic cancer CaSm model: up-regulation of CaSm is required for the transformed phenotype of murine pancreatic adenocarcinoma. Molecular therapy : the journal of the American Society of Gene Therapy 10 15727932
2005 Bystander effect contributes to the antitumor efficacy of CaSm antisense gene therapy in a preclinical model of advanced pancreatic cancer. Molecular therapy : the journal of the American Society of Gene Therapy 10 16226492
2013 The cellular decapping activators LSm1, Pat1, and Dhh1 control the ratio of subgenomic to genomic Flock House virus RNAs. Journal of virology 9 23536653
2022 mRNA Decapping Factors LSM1 and PAT Paralogs Are Involved in Turnip Mosaic Virus Viral Infection. Molecular plant-microbe interactions : MPMI 8 35100808
2022 The cytoplasmic LSm1-7 and nuclear LSm2-8 complexes exert opposite effects on Hepatitis B virus biosynthesis and interferon responses. Frontiers in immunology 8 36016928
2019 Homozygous noncanonical splice variant in LSM1 in two siblings with multiple congenital anomalies and global developmental delay. Cold Spring Harbor molecular case studies 7 31010896
2022 LSM1 is the new candidate gene for neurodevelopmental disorder. European journal of medical genetics 6 36100156
1997 Lineage- and differentiation stage-specific expression of LSM-1 (LPAP), a possible substrate for CD45, in human hematopoietic cells. American journal of hematology 6 8980254
2024 CASM mediates LRRK2 recruitment and activation under lysosomal stress. Autophagy 4 38497477
2023 TECPR1 helps bridge the CASM during lysosome damage. The EMBO journal 4 37638605
1990 Serum and serotonin induce retraction of calf aortic smooth muscle (CASM) cells in vitro: inhibition by ketanserin, a 5-HT2 receptor antagonist. Experimental cell research 4 2298230
2025 BLTP3A is associated with membranes of the late endocytic pathway and is an effector of CASM. bioRxiv : the preprint server for biology 3 39386594
2023 Correlation between LSM1 Expression and Clinical Outcomes in Glioblastoma and Functional Mechanisms. International journal of genomics 3 37954131
2025 BLTP3A is associated with membranes of the late endocytic pathway and is an effector of CASM. The EMBO journal 2 40935891
2024 Individual Atg8 paralogs and a bacterial metabolite sequentially promote hierarchical CASM-xenophagy induction and transition. Cell reports 2 38656870
2023 The separate axes of TECPR1 and ATG16L1 in CASM. Autophagy 2 37676042
2025 LSM1 c.231+4A>C hotspot variant is associated with a novel neurodevelopmental syndrome: first patient cohort. Journal of medical genetics 1 40204357
2026 The Lsm1-7 complex couples 3'-end protection to histone H4 acetylation to maintain mRNA homeostasis in Fusarium graminearum. Nucleic acids research 0 41805128
2016 Mutagenic Analysis of the C-Terminal Extension of Lsm1. PloS one 0 27434131