Affinage

KIF23

Kinesin-like protein KIF23 · UniProt Q02241

Length
960 aa
Mass
110.1 kDa
Annotated
2026-04-28
79 papers in source corpus 28 papers cited in narrative 29 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

KIF23 (MKLP1/CHO1) is a kinesin-6 family motor protein that functions as an essential organizer of the central spindle and midbody during cytokinesis, with additional postmitotic roles in establishing nonuniform microtubule polarity in neurons and podocytes. It forms the centralspindlin heterotetramer with CYK-4/MgcRacGAP, which is necessary and sufficient for antiparallel microtubule bundling (PMID:17942600), and its proper function requires phosphorylation by PLK1 at Ser904/905 (PMID:15199097), Aurora B on C-terminal serines (PMID:15854913), and NDR/LATS kinases at S716/S814 controlling 14-3-3 binding and clustering (PMID:25658096), as well as INCENP-dependent recruitment to the midzone (PMID:15796717) and Arf6 interaction at the midbody via a structurally defined 2:2 heterotetramer that bridges microtubule bundles to membranes (PMID:22522702). KIF23 transcription is cell-cycle regulated through the p53/p21/DREAM-MMB/CHR axis (PMID:23650552) and CUX1/E2F1 (PMID:19015243), while its mRNA is post-transcriptionally stabilized by NAT10-mediated ac4C modification (PMID:36522719) and its protein by SIRT7-mediated desuccinylation (PMID:38360598). A missense mutation (p.P916R) in KIF23 causes autosomal dominant congenital dyserythropoietic anemia type III through cytokinesis failure (PMID:23570799).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 1995 High

    Identifying PLK1 as a mitotic-phase interactor and kinase for KIF23 established the first regulatory connection between a mitotic kinase and a kinesin motor at the midbody, explaining how cytokinesis-specific motor activation might be achieved.

    Evidence Co-IP, in vitro kinase assay, and immunofluorescence colocalization in mammalian cells

    PMID:8524282

    Open questions at the time
    • Specific phosphorylation sites not mapped
    • Functional consequence of PLK1 phosphorylation on cytokinesis not tested
  2. 1998 High

    Demonstrating that KIF23 is expressed postmitotically in neurons and podocytes, and is required for nonuniform microtubule polarity in podocyte processes, revealed a cell-division-independent function for this motor in organizing microtubule arrays for cell morphogenesis.

    Evidence Antisense oligonucleotide knockdown in podocytes with hook-decoration MT polarity assay; in situ hybridization and expression analysis in rodent neurons

    PMID:9749792 PMID:9864367

    Open questions at the time
    • No direct loss-of-function in neurons at this stage
    • Mechanism by which KIF23 establishes minus-end-distal polarity not defined
  3. 1999 High

    Discovery that GTP-bound Arf proteins directly bind the KIF23 C-terminal tail provided the first link between this motor and membrane trafficking machinery, suggesting a mechanism for coupling microtubule organization to membrane delivery at the cleavage furrow.

    Evidence Yeast two-hybrid, GST pull-down with Arf switch-region mutants

    PMID:10506747

    Open questions at the time
    • Structural basis of the interaction unknown
    • In vivo relevance for cytokinesis not tested
  4. 2004 High

    Mapping PLK1 phosphorylation to Ser904/905 and demonstrating that non-phosphorylatable KIF23 causes cytokinesis failure established that PLK1-mediated phosphorylation is functionally required, not merely correlative.

    Evidence In vitro kinase assay with phosphosite mutagenesis, siRNA knockdown, rescue experiments in mammalian cells

    PMID:15199097

    Open questions at the time
    • Whether PLK1 phosphorylation regulates motor activity vs. localization vs. protein interactions not distinguished
  5. 2005 High

    Identifying Aurora B as a second essential kinase for KIF23 — phosphorylating C-terminal serines required for cytokinesis completion but not midzone localization — separated localization from activation and revealed a two-kinase regulatory logic; simultaneously, INCENP was shown to be required for KIF23 midzone recruitment, defining the recruitment pathway.

    Evidence In vitro kinase assay, phosphosite mutagenesis, Aurora kinase inhibitor in C. elegans and human cells; siRNA knockdowns with live-cell imaging and 3D reconstruction

    PMID:15796717 PMID:15854913

    Open questions at the time
    • Substrates downstream of KIF23 phosphorylation at these sites not identified
    • How INCENP recruits KIF23 molecularly not resolved
  6. 2006 Medium

    Defining the motor and tail domain requirements for dendritic targeting of KIF23, and identifying two NLS sequences required for interphase nuclear import regulated by phosphorylation, revealed how domain architecture controls both neuronal and cell-cycle-dependent KIF23 trafficking.

    Evidence GFP-tagged domain deletion mutants in hippocampal neurons; NLS mutagenesis with cell cycle analysis in mammalian cells

    PMID:16418225 PMID:17198681

    Open questions at the time
    • Identity of the kinase(s) phosphorylating the NLS in vivo unknown
    • No loss-of-function phenotype for NLS-deleted KIF23 in neurons
  7. 2007 High

    Biochemical reconstitution of centralspindlin as a CYK-4/KIF23 heterotetramer that is necessary and sufficient for microtubule bundling resolved the stoichiometry and established centralspindlin — not KIF23 alone — as the functional unit for central spindle organization.

    Evidence In vitro reconstitution, microtubule bundling assay, conditional genetics and suppressor screen in C. elegans

    PMID:17942600

    Open questions at the time
    • High-resolution structure of the full complex not available
    • How centralspindlin is targeted specifically to antiparallel overlaps in vivo not resolved
  8. 2008 High

    Demonstrating that CUX1 and E2F1 cooperatively activate the KIF23 promoter upon S-phase entry established the transcriptional basis for cell-cycle-dependent KIF23 expression.

    Evidence ChIP, promoter-luciferase assays, overexpression and siRNA of CUX1/E2F1

    PMID:19015243

    Open questions at the time
    • Whether CUX1/E2F1 regulation is sufficient in all cell types not tested
  9. 2012 High

    The crystal structure of the Arf6–KIF23 complex as a 2:2 heterotetramer with a membrane-binding β-sheet surface provided a structural mechanism for how the motor bridges microtubule bundles to membranes at the cleavage furrow.

    Evidence X-ray crystallography, structure-based mutagenesis, siRNA knockdowns

    PMID:22522702

    Open questions at the time
    • Membrane lipid specificity of the complex not defined
    • How Arf6 activation is spatially coupled to KIF23 localization in vivo not resolved
  10. 2013 High

    Identification of the KIF23 p.P916R mutation as the cause of autosomal dominant CDA III, validated by rescue experiments showing cytokinesis failure, linked the motor's molecular function to human disease and erythropoiesis.

    Evidence Targeted sequencing, haplotype analysis in families, RNAi knockdown and mutant rescue in HeLa cells

    PMID:23570799

    Open questions at the time
    • Structural/biochemical mechanism by which P916R disrupts function not determined
    • No erythroid-specific cellular model used
  11. 2013 Medium

    Demonstrating p53/p21/DREAM-MMB/CHR-dependent transcriptional repression of KIF23 established the tumor suppressor pathway controlling KIF23 expression and explained why KIF23 is upregulated in p53-deficient cancers.

    Evidence Promoter reporter assays with CHR mutagenesis, ChIP for DREAM/MMB complexes, qRT-PCR

    PMID:23650552

    Open questions at the time
    • Relative contributions of DREAM vs. CUX1/E2F1 not integrated
    • Whether CHR-mediated regulation occurs in non-transformed cells not confirmed
  12. 2015 High

    Identifying NDR/LATS kinases as regulators of sequential KIF23 phosphorylation (S716→S814) creating a 14-3-3 binding site revealed a Hippo-pathway-linked mechanism controlling KIF23 clustering during cytokinesis.

    Evidence In vitro kinase assays, phosphosite mutagenesis, 14-3-3 binding assays, in vivo phosphorylation analysis

    PMID:25658096

    Open questions at the time
    • Whether LATS kinase regulation of KIF23 connects to canonical Hippo pathway tumor suppression not explored
    • Functional consequence of 14-3-3 binding on motor activity or localization not fully resolved
  13. 2016 High

    Demonstrating that p120-catenin binds KIF23 at the cleavage furrow to spatially control RhoA cycling connected the adherens junction scaffold to cytokinetic RhoA regulation, explaining how cell-cell adhesion components influence division.

    Evidence Reciprocal Co-IP, domain mutagenesis, siRNA knockdown with multinucleation phenotype

    PMID:28004812

    Open questions at the time
    • Whether this mechanism operates in epithelial tissues in vivo not tested
    • Molecular details of how p120/KIF23 interaction modulates Ect2 or CYK-4 GAP activity unclear
  14. 2022 Medium

    Discovery that centralspindlin/Ect2 excludes NuMA/dynein from the equatorial membrane revealed a spatial antagonism mechanism by which KIF23-containing complexes define cleavage furrow identity by segregating cortical force generators.

    Evidence siRNA knockdowns, immunofluorescence of membrane domain compartmentalization, live-cell imaging

    PMID:36197340

    Open questions at the time
    • Biochemical basis for mutual exclusion between centralspindlin/Ect2 and NuMA/dynein complexes not defined
    • Whether this mechanism operates in asymmetric divisions not tested
  15. 2022 Medium

    Post-transcriptional regulation of KIF23 by NAT10-mediated ac4C mRNA modification and transcriptional activation by FOXM1 or TAZ-TEAD2 revealed multiple cancer-relevant pathways converging to elevate KIF23 expression.

    Evidence RIP-seq and acRIP-seq for NAT10/ac4C; ChIP for FOXM1 at KIF23 promoter; CRISPRi screen identifying KIF23 as TAZ-TEAD2 target

    PMID:36522719 PMID:36894036 PMID:36940637

    Open questions at the time
    • Whether these regulatory pathways operate independently or synergistically not determined
    • Relative contribution of transcriptional vs. post-transcriptional regulation to KIF23 protein levels in normal cells unknown
  16. 2024 High

    KIF23 depletion in mouse cortex disrupted spindle orientation and apical junction integrity in neural stem cells — rescued by wild-type but not a microcephaly variant — establishing KIF23 as required for cortical neurogenesis beyond its canonical cytokinesis role.

    Evidence In vivo knockdown in mouse cortex, rescue with WT vs. disease-variant human KIF23, live imaging, immunofluorescence

    PMID:39632980

    Open questions at the time
    • Whether spindle orientation defect is independent of the cytokinesis role not fully separated
    • Identity and mechanism of the microcephaly-associated variant not fully characterized

Open questions

Synthesis pass · forward-looking unresolved questions
  • The mechanism by which KIF23 motor activity is autoinhibited and relieved in vivo, the structural basis of full-length centralspindlin, and how KIF23's multiple post-translational modifications are integrated to coordinate cytokinesis progression remain unresolved.
  • No structure of full-length centralspindlin or autoinhibited KIF23
  • Integration of PLK1, Aurora B, and LATS phosphorylation events temporally and functionally not resolved
  • Autoinhibition mechanism rests on a retracted paper (PMID:33204722) and awaits independent confirmation

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003774 cytoskeletal motor activity 2 GO:0008092 cytoskeletal protein binding 2
Localization
GO:0005856 cytoskeleton 3 GO:0005634 nucleus 1
Pathway
R-HSA-1640170 Cell Cycle 6 R-HSA-162582 Signal Transduction 3
Partners
ARF6AURKBCTNND1INCENPMYH9PLK1RACGAP1SIRT7
Complex memberships
centralspindlin (CYK-4/MKLP1 heterotetramer)

Evidence

Reading pass · 29 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1995 PLK1 (Plk) physically interacts with CHO1/MKLP1 (KIF23) in vivo during late M phase, colocalizing at the midbody, and CHO1/MKLP1 is phosphorylated by Plk-associated kinase activity in vitro. CHO1/MKLP1 induces microtubule bundling and antiparallel movement in vitro. Co-immunoprecipitation, in vitro kinase assay, immunofluorescence colocalization Molecular and cellular biology High 8524282
2004 PLK1 binds CHO1/MKLP1 (KIF23) via its Polo-box domain, while the stalk domain of CHO1/MKLP1 mediates binding to PLK1. Ser904 and Ser905 are the two major PLK1 phosphorylation sites on CHO1/MKLP1. Depletion of CHO1/MKLP1 causes multinucleation and mislocalizes PLK1 during late mitosis; overexpression of a non-phosphorylatable mutant causes cytokinesis defects; rescue requires the phosphorylatable form. Transient transfection domain-mapping, in vitro kinase assay with phosphosite mutagenesis, siRNA knockdown, immunofluorescence, rescue experiments Journal of cell science High 15199097
2005 Aurora B phosphorylates MKLP1 (KIF23) on conserved C-terminal serine residues in vitro and in vivo. A non-phosphorylatable MKLP1 mutant localizes properly but fails to support completion of cytokinesis. Aurora B inhibition in late anaphase attenuates MKLP1 phosphorylation and causes cytokinesis failure without disrupting the central spindle. In vitro kinase assay, phosphosite mutagenesis, Aurora kinase inhibitor treatment, live-cell imaging, C. elegans genetics and human cell culture Current biology : CB High 15854913
2005 INCENP is required for recruiting MKLP1 (KIF23) to the spindle midzone/midbody. MKLP1 depletion by siRNA does not disrupt chromosome segregation or midzone formation but abrogates midbody formation and completion of cytokinesis. INCENP depletion disrupts midzone/midbody formation and causes binucleation. RNAi/siRNA knockdown, immunofluorescence, live-cell imaging, 3D reconstruction The Biochemical journal High 15796717
1999 GTP-bound (activated) Arf proteins directly bind the C-terminal tail domain of MKLP1 (KIF23) via switch I and switch II regions. An 88-amino acid domain in the MKLP1 C-terminus is sufficient for GTP-dependent Arf binding, and all human Arf isoforms interact with this domain. Yeast two-hybrid screen, deletion mapping, GST pull-down assay, point mutagenesis of Arf switch regions Cell motility and the cytoskeleton High 10506747
2012 Arf6 directly interacts with MKLP1 (KIF23) at the Flemming body/midbody. Crystal structure of the Arf6-MKLP1 complex shows MKLP1 forms a homodimer flanked by two Arf6 molecules (2:2 heterotetramer) with an extended β-sheet spanning the complex, suitable for membrane interaction at the cleavage furrow. Structure-based mutagenesis and siRNA knockdowns confirm that complex formation is required for completion of cytokinesis. Crystal structure determination, structure-based mutagenesis, siRNA knockdown, immunofluorescence The EMBO journal High 22522702
2007 Centralspindlin is a heterotetrameric complex composed of two CYK-4/MgcRacGAP homodimers (via parallel coiled coil) and two ZEN-4/MKLP1 homodimers (via parallel coiled coil), assembled through two low-affinity interactions. Centralspindlin (but not individual subunits alone) is sufficient to bundle microtubules in vitro. Biochemical dissection, in vitro microtubule bundling assay, conditional genetic mutations and suppressor screen in C. elegans Molecular biology of the cell High 17942600
2013 TRAF6 E3 ubiquitin ligase mediates ubiquitination of KIF23/MKLP1 at the midbody ring. SQSTM1/p62 and WDFY3/ALFY form a complex with TRAF6, and these proteins plus NBR1 are required for clearance of midbody ring derivatives by selective autophagy. TRAF6 depletion reduces ubiquitinated midbody ring derivatives. siRNA knockdown, immunofluorescence, autophagy assays Autophagy Medium 24128730
2013 A missense mutation in KIF23 (p.P916R) causes autosomal dominant congenital dyserythropoietic anemia type III (CDA III). RNAi-based knockdown and rescue experiments in HeLa cells demonstrate that the p.P916R mutation causes cytokinesis failure, consistent with large multinucleated erythroblasts observed in patients. Targeted next-generation sequencing, RNAi knockdown, mutant rescue in HeLa cells, haplotype analysis Blood High 23570799
1998 CHO1/MKLP1 (KIF23) is required for establishing nonuniform microtubule polarity in podocyte processes. Antisense oligonucleotide treatment in differentiating podocytes abolishes both process formation and nonuniform MT polarity. CHO1/MKLP1 associates with microtubules in podocytes during process formation. Antisense oligonucleotide treatment, hook-decoration MT polarity assay, immunofluorescence, taxol/nocodazole recovery experiments The Journal of cell biology High 9864367
1998 CHO1/MKLP1 (KIF23) is expressed in postmitotic neurons (in vivo and in vitro), with mRNA levels peaking prior to dendritic development and declining after dendrites differentiate. Dorsal root ganglion neurons (axon-only) express significantly lower levels, consistent with a role in establishing the dendritic minus-end-distal microtubule polarity pattern. In situ hybridization in rodent brain, in vitro hippocampal neuron culture, quantitative expression analysis The European journal of neuroscience Medium 9749792
2006 MKLP1 (KIF23) nuclear localization during interphase requires two NLS sequences (899SRKRRSST906 and 949KRKKP953) in its tail domain. Expression of an NLS-deleted mutant causes cell cycle arrest at cytokinesis. Phosphomimetic mutations at two serines within the first NLS attenuate nuclear localization, suggesting phosphorylation-regulated nuclear import. Domain mapping by mutagenesis, ectopic expression of NLS mutants, cell cycle analysis Biochemical and biophysical research communications Medium 17198681
2006 The motor and tail domains of MKLP1 (KIF23) are required for its dendritic targeting in hippocampal neurons. Deletion of the motor domain prevents dendritic distribution; deletion of the tail domain causes axonal mislocalization; deletion of the stalk domain still permits dendritic targeting. eGFP-tagged domain deletion mutants expressed in primary hippocampal neurons, fluorescence microscopy Journal of cell science Medium 16418225
2015 Kif23 isoform CHO1 (but not the shorter MKLP1 isoform) contains two NDR/LATS kinase consensus sites (S716 and S814) that are phosphorylated by NDR and LATS kinases in vitro. Phosphorylation at S814 creates a 14-3-3 binding site important for Kif23 clustering during cytokinesis. Sequential phosphorylation occurs: S716 phosphorylation is required for efficient S814 phosphorylation. LATS1/2 participate in S814 phosphorylation in vivo. In vitro kinase assay with NDR/LATS, site-directed mutagenesis, 14-3-3 binding assay, in vivo phosphorylation analysis PloS one High 25658096
2016 p120-catenin binds MKLP1 (KIF23) via its N-terminal coiled-coil domain (isoform 1A) at the cleavage furrow during anaphase to spatially control RhoA GTPase cycling. This binding is independent of cadherin association. Loss of p120 leads to multinucleation due to cytokinesis failure. Co-immunoprecipitation, domain mutagenesis, immunofluorescence, siRNA knockdown with multinucleation readout Nature communications High 28004812
2013 KIF23 transcription is regulated by p53 via p21(WAF1/CIP1)-dependent repression. The cell cycle genes homology region (CHR) element in the KIF23 promoter is required for both p53-dependent repression and cell cycle-dependent expression. DREAM and MMB complexes differentially bind the CHR element to control cell cycle- and p53-dependent regulation of KIF23. Promoter reporter assays, qRT-PCR, Western blot, chromatin immunoprecipitation PloS one Medium 23650552
2008 CUX1 and E2F1 transcription factors cooperatively bind the MKLP1 (KIF23) promoter upon S-phase entry, driving its transcriptional activation. The KIF23 promoter contains CHR sites, E2F binding elements, and CUX1 binding sites. CUX1 or E2F1 overexpression increases endogenous MKLP1 protein; E2F1 knockdown reduces it. Chromatin immunoprecipitation, promoter-luciferase reporter assays, dominant negative/overexpression of transcription factors, siRNA Molecular and cellular biology High 19015243
2024 SIRT7 physically interacts with KIF23 and inhibits its succinylation at K537, thereby enhancing KIF23 protein stability. Overexpression of SIRT7 increases KIF23 protein levels in HEK-293T cells, and SIRT7 knockdown blocks the pro-proliferative and pro-migratory effects of KIF23 overexpression in anaplastic thyroid cancer cells. Co-immunoprecipitation, Western blot for succinylation, protein stability assay, siRNA knockdown, overexpression BMC cancer Medium 38360598
2020 KIF23 activates Wnt/β-catenin signaling in gastric cancer by directly interacting with Amer1, competitively blocking Amer1-APC association, and relocating Amer1 from the membrane/cytoplasm to the nucleus, thereby attenuating Amer1's negative regulation of Wnt/β-catenin signaling. Co-immunoprecipitation, immunofluorescence, siRNA knockdown, cell function assays, TCGA/GEO data analysis Aging Medium 32365332
2020 The tail domain of MKLP1 (KIF23) exhibits an autoinhibitory effect on its motor activity; overexpression of the tail domain alone blocks cytokinesis and causes binucleation. PAK2 (p21-activated kinase 2) binds the MKLP1 tail domain, and PAK2 knockdown causes loss of MKLP1 midbody localization and cytokinesis failure, suggesting PAK2 binding relieves autoinhibition. GST pull-down, LC-MS/MS interactome, co-immunoprecipitation, FRET, siRNA knockdown, overexpression in HEK293 cells BioMed research international Low 33204722
2022 FOXM1 epigenetically activates KIF23 expression in hepatocellular carcinoma by increasing RNA polymerase II occupancy and histone H3K27 acetylation at the KIF23 promoter. FOXM1 suppression reduces KIF23 levels and reverses sorafenib resistance. ChIP for RNA Pol II and H3K27ac at KIF23 promoter, Western blot, siRNA knockdown Biochemical and biophysical research communications Medium 36940637
2022 NAT10 binds the 3'UTR of KIF23 mRNA and up-regulates its ac4C modification, stabilizing KIF23 mRNA and increasing KIF23 protein levels, which in turn activates Wnt/β-catenin signaling via GSK-3β/β-catenin to promote colorectal cancer progression. RIP-seq, acRIP-seq, RNA stability assays, luciferase reporter assay, Western blot, in vitro and in vivo cancer models Journal of experimental & clinical cancer research : CR Medium 36522719
2024 KIF23 depletion in mice disrupts mitotic spindle orientation and impairs cytokinesis in neural stem and progenitor cells (NSPCs), leading to precocious neurogenesis, neuronal apoptosis, accelerated cell cycle exit, and disrupted apical junction protein localization. Phenotypes are rescued by wild-type human KIF23 but not by a microcephaly-associated variant, establishing KIF23 as required for spindle orientation and apical surface maintenance in cortical NSPCs. Kif23 knockdown in mouse cortex, rescue with WT vs. disease-variant human KIF23, live imaging, immunofluorescence for spindle orientation and apical junction proteins The EMBO journal High 39632980
2022 Centralspindlin (Cyk4/MKLP1) and its partner RhoGEF Ect2 are required for exclusion of NuMA/dynein/dynactin from the equatorial cell membrane during anaphase. Ect2-based and NuMA-based complexes occupy mutually exclusive membrane domains; equatorial enrichment of the Ect2/Cyk4/Mklp1 complex is essential for proper spindle elongation and cleavage furrow formation. siRNA knockdown, immunofluorescence, live-cell imaging of membrane compartmentalization The Journal of cell biology Medium 36197340
2025 Kif23 promotes cardiac fibroblast proliferation and myofibroblast transdifferentiation following myocardial infarction by activating RhoA and suppressing Ces1d-mediated fatty acid β-oxidation via the RhoA/ROCK1 signaling axis, causing pathological lipid accumulation and fibrosis. Kif23 knockdown (via AAV-shRNA) attenuated post-MI fibrosis in mice. AAV-mediated shRNA knockdown in vivo, TGF-β1-stimulated cardiac fibroblasts, proteomic profiling, lipid droplet analysis, Western blot for RhoA/ROCK1 Hypertension (Dallas, Tex. : 1979) Medium 41078122
2023 In hepatocellular carcinoma, the TAZ-TEAD2 transcriptional pathway promotes tumor cell proliferation via increased expression of KIF23 (and ANLN) as direct transcriptional targets. CRISPRi knockdown of KIF23 in mice reduced HCC tumor growth, and TAZ deletion consistently decreased HCC growth and mortality. CRISPRi screen, ChIP to confirm TAZ target genes, RNA sequencing, genetic deletion of TAZ in floxed mice, AAV8-Cre delivery Gastroenterology Medium 36894036
2016 In Drosophila motoneurons, Toll-6/dSARM/FoxO signaling represses Pavarotti/MKLP1 (ortholog of KIF23) expression. Elevated MKLP1 attenuates microtubule dynamics. Pathway loss-of-function phenotypes in axon transport and structural plasticity are rescued by reducing Pav-KLP expression, placing MKLP1 downstream of Toll-6-FoxO signaling. Drosophila genetic epistasis (suppressor analysis), in vivo imaging, Western blot The Journal of cell biology Medium 27502486
2026 The C-terminal domain of KIF23 directly binds the myosin tail domain of MYH9. This interaction stabilizes MYH9 by recruiting deubiquitinase USP7 to remove K48-linked ubiquitin chains from MYH9. Stabilized MYH9 then recruits USP15 to deubiquitinate MCM2 at K469, preventing MCM2 degradation. Elevated MCM2 enhances binding to PCNA, promoting cervical cancer cell proliferation and cisplatin resistance. Co-immunoprecipitation, CRISPR/Cas9 knockout, protein half-life assay, ubiquitination assay, domain mapping Clinical and translational medicine Medium 41940421
2025 CDK1 and Aurora B phosphorylation of Citron kinase (CIT-K) at S440 and S699 reduces CIT-K's ability to interact with its midbody partners including KIF23/MKLP1 (and KIF14 and AURKB), thereby regulating midbody formation and post-mitotic midbody remnant stability. Phosphosite mutagenesis, Co-immunoprecipitation, in vitro kinase assay, live-cell imaging bioRxivpreprint Medium

Source papers

Stage 0 corpus · 79 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1995 Plk is an M-phase-specific protein kinase and interacts with a kinesin-like protein, CHO1/MKLP-1. Molecular and cellular biology 245 8524282
2005 Phosphorylation of ZEN-4/MKLP1 by aurora B regulates completion of cytokinesis. Current biology : CB 179 15854913
2022 Acetyltransferase NAT10 regulates the Wnt/β-catenin signaling pathway to promote colorectal cancer progression via ac4C acetylation of KIF23 mRNA. Journal of experimental & clinical cancer research : CR 131 36522719
2007 Cooperative assembly of CYK-4/MgcRacGAP and ZEN-4/MKLP1 to form the centralspindlin complex. Molecular biology of the cell 94 17942600
2005 Recruitment of MKLP1 to the spindle midzone/midbody by INCENP is essential for midbody formation and completion of cytokinesis in human cells. The Biochemical journal 82 15796717
2011 Downregulation of KIF23 suppresses glioma proliferation. Journal of neuro-oncology 80 21904957
2015 Overexpression of KIF23 predicts clinical outcome in primary lung cancer patients. Lung cancer (Amsterdam, Netherlands) 79 26775597
2004 Molecular interactions of Polo-like-kinase 1 with the mitotic kinesin-like protein CHO1/MKLP-1. Journal of cell science 78 15199097
2013 Congenital dyserythropoietic anemia type III (CDA III) is caused by a mutation in kinesin family member, KIF23. Blood 74 23570799
2023 A Therapeutically Targetable TAZ-TEAD2 Pathway Drives the Growth of Hepatocellular Carcinoma via ANLN and KIF23. Gastroenterology 58 36894036
1999 Arf proteins bind to mitotic kinesin-like protein 1 (MKLP1) in a GTP-dependent fashion. Cell motility and the cytoskeleton 58 10506747
2012 Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. The EMBO journal 51 22522702
2013 TRAF6 mediates ubiquitination of KIF23/MKLP1 and is required for midbody ring degradation by selective autophagy. Autophagy 48 24128730
2019 KIF23 Promotes Gastric Cancer by Stimulating Cell Proliferation. Disease markers 46 31007778
2013 p53 and cell cycle dependent transcription of kinesin family member 23 (KIF23) is controlled via a CHR promoter element bound by DREAM and MMB complexes. PloS one 45 23650552
1998 Nonuniform microtubular polarity established by CHO1/MKLP1 motor protein is necessary for process formation of podocytes. The Journal of cell biology 45 9864367
2004 ZEN-4/MKLP1 is required to polarize the foregut epithelium. Current biology : CB 44 15182666
2008 CUX1 and E2F1 regulate coordinated expression of the mitotic complex genes Ect2, MgcRacGAP, and MKLP1 in S phase. Molecular and cellular biology 43 19015243
2016 An important role for Myb-MuvB and its target gene KIF23 in a mouse model of lung adenocarcinoma. Oncogene 39 27212033
2019 Methylation-mediated repression of MiR-424/503 cluster promotes proliferation and migration of ovarian cancer cells through targeting the hub gene KIF23. Cell cycle (Georgetown, Tex.) 38 31135262
2020 KIF23 enhances cell proliferation in pancreatic ductal adenocarcinoma and is a potent therapeutic target. Annals of translational medicine 37 33313139
2020 KIF23 activated Wnt/β-catenin signaling pathway through direct interaction with Amer1 in gastric cancer. Aging 36 32365332
2013 v-Src causes delocalization of Mklp1, Aurora B, and INCENP from the spindle midzone during cytokinesis failure. Experimental cell research 36 23562843
1998 Expression of the mitotic motor protein CHO1/MKLP1 in postmitotic neurons. The European journal of neuroscience 36 9749792
2016 A Toll receptor-FoxO pathway represses Pavarotti/MKLP1 to promote microtubule dynamics in motoneurons. The Journal of cell biology 35 27502486
2020 Knockdown of lncRNA PVT1 inhibits prostate cancer progression in vitro and in vivo by the suppression of KIF23 through stimulating miR-15a-5p. Cancer cell international 31 32624708
2016 Kinesin family members KIF11 and KIF23 as potential therapeutic targets in malignant pleural mesothelioma. International journal of oncology 30 27279560
2021 KIF23 promotes triple negative breast cancer through activating epithelial-mesenchymal transition. Gland surgery 29 34268078
2002 Zebrafish mitotic kinesin-like protein 1 (Mklp1) functions in embryonic cytokinesis. Physiological genomics 29 11842131
2006 The nuclear localization signal of mitotic kinesin-like protein Mklp-1: effect on Mklp-1 function during cytokinesis. Biochemical and biophysical research communications 26 17198681
2021 Aberrant KIF23 expression is associated with adverse clinical outcome and promotes cellular malignant behavior through the Wnt/β-catenin signaling pathway in Colorectal Cancer. Journal of Cancer 23 33754001
2016 p120-catenin prevents multinucleation through control of MKLP1-dependent RhoA activity during cytokinesis. Nature communications 23 28004812
2024 SIRT7 promotes the proliferation and migration of anaplastic thyroid cancer cells by regulating the desuccinylation of KIF23. BMC cancer 20 38360598
2017 Mutation analysis and copy number alterations of KIF23 in non-small-cell lung cancer exhibiting KIF23 over-expression. OncoTargets and therapy 18 29066916
2021 Functional roles of the SHCBP1 and KIF23 interaction in modulating the cell-cycle and cisplatin resistance of head and neck squamous cell carcinoma. Head & neck 16 34918847
2006 MKLP1 requires specific domains for its dendritic targeting. Journal of cell science 15 16418225
2023 KIF23, under regulation by androgen receptor, contributes to nasopharyngeal carcinoma deterioration by activating the Wnt/β-catenin signaling pathway. Functional & integrative genomics 14 37010644
2021 MiR-17-5p downregulation alleviates apoptosis and fibrosis in high glucose-induced human mesangial cells through inactivation of Wnt/β-catenin signaling by targeting KIF23. Environmental toxicology 13 34014023
2017 Temporal regulation of epithelium formation mediated by FoxA, MKLP1, MgcRacGAP, and PAR-6. Molecular biology of the cell 13 28539408
2015 Binding of Kif23-iso1/CHO1 to 14-3-3 is regulated by sequential phosphorylations at two LATS kinase consensus sites. PloS one 13 25658096
2022 Knockdown of circ_0067934 inhibits gastric cancer cell proliferation, migration and invasion via the miR‑1301‑3p/KIF23 axis. Molecular medicine reports 12 35475447
2022 KIF23 promotes autophagy-induced imatinib resistance in chronic myeloid leukaemia through activating Wnt/β-catenin pathway. Clinical and experimental pharmacology & physiology 12 36066385
2020 Kinesin family member 23 (KIF23) contributes to the progression of bladder cancer cells in vitro and in vivo. Neoplasma 12 33231086
2008 Zygotic loss of ZEN-4/MKLP1 results in disruption of epidermal morphogenesis in the C. elegans embryo. Developmental dynamics : an official publication of the American Association of Anatomists 12 18265015
2024 KIF23 promotes cervical cancer progression via inhibiting NLRP3-mediated pyroptosis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 11 38780518
1999 Nuclear localization of C-terminal domains of the kinesin-like protein MKLP-1. Biochemical and biophysical research communications 11 10403813
2023 FOXM1 augments sorafenib resistance and promotes progression of hepatocellular carcinoma by epigenetically activating KIF23 expression. Biochemical and biophysical research communications 10 36940637
2022 Inhibition of KIF23 Alleviates IPAH by Targeting Pyroptosis and Proliferation of PASMCs. International journal of molecular sciences 10 35457254
2021 Systematic Pan-Cancer Analysis of KIF23 and a Prediction Model Based on KIF23 in Clear Cell Renal Cell Carcinoma (ccRCC). Pharmacogenomics and personalized medicine 10 35002290
2021 Mutation and Copy Number Alterations Analysis of KIF23 in Glioma. Frontiers in genetics 9 34017355
2024 Mechanism of regulation of KIF23 on endometrial cancer cell growth and apoptosis. Discover oncology 8 38514510
2024 Kinesin-like motor protein KIF23 maintains neural stem and progenitor cell pools in the developing cortex. The EMBO journal 8 39632980
2022 KIF23 is a potential biomarker of diffuse large B cell lymphoma: Analysis based on bioinformatics and immunohistochemistry. Medicine 8 35713434
2020 Congenital dyserythropoietic anemia types Ib, II, and III: novel variants in the CDIN1 gene and functional study of a novel variant in the KIF23 gene. Annals of hematology 8 33159567
2024 Regulation of KIF23 by miR-107 controls replicative tumor cell fitness in mouse and human hepatocellular carcinoma. Journal of hepatology 7 40235270
2022 DEP domain containing 1B (DEPDC1B) exerts the tumor promoter in hepatocellular carcinoma through activating p53 signaling pathway via kinesin family member 23 (KIF23). Bioengineered 7 34983303
2024 Identification and validation of KIF23 as a hypoxia-regulated lactate metabolism-related oncogene in uterine corpus endometrial carcinoma. Life sciences 6 38336274
2022 Membrane compartmentalization of Ect2/Cyk4/Mklp1 and NuMA/dynein regulates cleavage furrow formation. The Journal of cell biology 6 36197340
2025 KIF23 inhibition protects against perioperative neurocognitive disorders by hindering ROS/caspase-3/GSDME-mediated pyroptosis. Experimental neurology 5 40639436
2024 Knockdown of KIF23 alleviates the progression of asthma by inhibiting pyroptosis. BMJ open respiratory research 5 38569671
2020 Differential tissue specific expression of Kif23 alternative transcripts in mice with the human mutation causing congenital dyserythropoietic anemia type III. Blood cells, molecules & diseases 5 32818800
2020 Revealing PAK2's Function in the Cell Division through MKLP1's Interactome. BioMed research international 5 33204722
2023 KCNQ1OT1 promotes retinoblastoma progression by targeting miR-339-3p that suppresses KIF23. International ophthalmology 3 37198502
2019 Inhibition of neddylation induces mitotic defects and alters MKLP1 accumulation at the midbody during cytokinesis. Cell cycle (Georgetown, Tex.) 3 31057046
1998 Sequence and expression of DmMKLP1, a homolog of the human MKLP1 kinesin-like protein from Drosophila melanogaster. Development genes and evolution 3 9799428
2025 Cancer-associated fibroblast-derived exosomal FAM83F regulates KIF23 expression to promote the malignant progression and reduce radiosensitivity in non-small cell lung cancer. Cytotechnology 2 39867833
2025 Kif23 Promotes Myocardial Fibrosis by Suppressing Ces1d-Dependent Lipid Metabolism. Hypertension (Dallas, Tex. : 1979) 2 41078122
2025 ETV5 transcriptionally inhibits KIF23 to repress pyroptosis in aged mice with perioperative neurocognitive disorders. Biochemical pharmacology 1 40983152
2024 Molecular structure of polysaccharide mediated autophagy markers KIF23 and PRC1 proteins and their regulatory role in triple negative cancer through the p53 signaling pathway. International journal of biological macromolecules 1 39725112
2026 KIF23 Overexpression Promotes Cell Viability, Migration, and Invasion via the Wnt/β-Catenin Signaling Pathway in Anaplastic Thyroid Carcinoma. International journal of endocrinology 0 41675939
2026 NCBP2 Regulates PGAM5-Mediated Mitophagy Via KIF23 Alternative Splicing To Promote Cervical Cancer ProgressionRun Title: NCBP2 Promotes Cervical Cancer Via Mitophagy. Applied biochemistry and biotechnology 0 41874817
2026 Targeting KIF23 inhibits cell proliferation and primary chemoresistance in cervical cancer by inactivating the MYH9/MCM2/PCNA pathway. Clinical and translational medicine 0 41940421
2025 RETRACTION: KIF23 Promotes Gastric Cancer by Stimulating Cell Proliferation. Disease markers 0 40726601
2025 Assessment of anti-cancer activity of cyclovirobuxine D in nasopharyngeal carcinoma cells: Involvement of KIF23-mediated Akt/mTOR pathway. Pathology, research and practice 0 40902449
2025 Congenital Dyserythropoietic Anemia Type III Associated With a Novel KIF23 Variant (c.2132A>G; p.Gln711Arg): A Case Report. Clinical case reports 0 40927401
2025 KIF23 silencing suppresses papillary thyroid carcinoma metastasis by regulating mitophagy via Wnt/β-catenin pathway. Endocrine connections 0 41138746
2025 A core stemness-associated module reveals PLK1, NUF2, KIF23, CDCA8, TOP2A, CENPF, AURKA, and ASPM as key genes in rectal cancer. European journal of medical research 0 41373021
2024 Retracted: Revealing PAK2's Function in the Cell Division through MKLP1's Interactome. BioMed research international 0 38550036
2024 [Corrigendum] Knockdown of circ_0067934 inhibits gastric cancer cell proliferation, migration and invasion via the miR‑1301‑3p/KIF23 axis. Molecular medicine reports 0 39155879