Affinage

KIF17

Kinesin-like protein KIF17 · UniProt Q9P2E2

Length
1029 aa
Mass
115.1 kDa
Annotated
2026-04-28
61 papers in source corpus 31 papers cited in narrative 31 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

KIF17 is a homodimeric kinesin-2 family motor protein that mediates plus-end-directed microtubule transport of diverse cargoes in neuronal dendrites, primary cilia, and specialized cell types including photoreceptors and spermatids. In neurons, KIF17 transports NR2B-containing NMDA receptor vesicles, Kv4.2 potassium channels, and kainate receptor subunits into dendrites by binding adaptor proteins such as mLin-10/Mint1 through its C-terminal tail domain; cargo loading relieves autoinhibition to initiate motility, while CaMKII phosphorylation of Ser1029 disrupts the KIF17–Mint1 interaction to trigger cargo release, and dendritic selectivity is enforced by actin-based filtering at the axon initial segment and dynein-mediated redirection (PMID:10846156, PMID:18066053, PMID:27265394, PMID:16257958). Kif17 knockout mice exhibit reduced NR2B expression, impaired NMDA receptor-mediated synaptic plasticity, and hippocampus-dependent memory deficits, while activity-dependent local dendritic synthesis of KIF17 via its 3′UTR is required for fear memory extinction (PMID:21521616, PMID:33328231). In non-neuronal contexts, KIF17 enters primary cilia through a RanGTP/importin-β2-regulated ciliary localization signal and IFT-B complex interaction via IFT46–IFT56, where it participates in intraflagellar transport, ciliary receptor targeting, photoreceptor outer segment development, CaMKII-dependent disc shedding, and Hedgehog pathway modulation through cell-type-specific regulation of GLI processing and SHH levels (PMID:20526328, PMID:28077622, PMID:18304522, PMID:30458707, PMID:38669326).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 2000 High

    Identification of KIF17 as the motor for NR2B vesicle transport answered how NMDA receptors reach dendrites: KIF17 directly binds mLin-10/Mint1 via its tail domain, linking it to a mLin-2/mLin-7/NR2B complex for microtubule-based dendritic delivery.

    Evidence Co-immunoprecipitation, direct binding pulldown, and in vitro microtubule transport assay in neurons

    PMID:10846156

    Open questions at the time
    • Stoichiometry of the KIF17–mLin-10–NR2B complex not determined
    • Whether other adaptors can substitute for mLin-10 in vivo unknown
  2. 2002 High

    Transgenic overexpression of KIF17 in mice enhanced NR2B expression, CREB phosphorylation, and spatial/working memory, establishing in vivo physiological relevance of KIF17-mediated NR2B transport for cognition.

    Evidence KIF17 transgenic mice with behavioral tasks, Western blotting, immunohistochemistry

    PMID:12391294

    Open questions at the time
    • Overexpression may have gain-of-function artifacts beyond NR2B trafficking
    • Cell-type specificity of the memory enhancement not resolved
  3. 2003 High

    Live imaging and knockdown experiments revealed that KIF17 vesicles move processively at ~0.76 µm/s in dendrites and that KIF17 loss reduces NR2B with compensatory NR2A increases, defining the functional dynamics of this transport and its impact on NMDAR subunit composition.

    Evidence Live-cell imaging, dominant-negative and siRNA knockdown in hippocampal neurons

    PMID:12514209

    Open questions at the time
    • Mechanism of compensatory NR2A upregulation not established
  4. 2003 Medium

    Discovery that the testis-specific KIF17b isoform transports CREM-regulated mRNAs in an RNA–protein complex with TB-RBP/Translin extended KIF17 function beyond receptor trafficking to mRNP transport in male germ cells.

    Evidence Co-immunoprecipitation, in vivo RNA crosslinking, in situ hybridization with immunohistochemistry at EM level in spermatids

    PMID:14673085

    Open questions at the time
    • Direct motor activity for mRNP transport not reconstituted in vitro
    • Which specific mRNAs are KIF17b-dependent not comprehensively defined
  5. 2005 High

    Identification of Kv4.2 as a second dendritic cargo demonstrated that KIF17 is a general dendritic ion channel transporter, not specific to NMDA receptors.

    Evidence Co-immunoprecipitation from brain lysate, dominant-negative KIF17 blocking Kv4.2 dendritic localization in cortical neurons

    PMID:16257958

    Open questions at the time
    • Adaptor linking KIF17 to Kv4.2 not identified
    • Whether Kv4.2 transport shares the CaMKII-dependent release mechanism unknown
  6. 2006 High

    Three discoveries broadened KIF17's role to ciliary transport and additional neuronal cargoes: KIF17 is required for olfactory CNG channel ciliary targeting, transports kainate receptor subunits in dendrites, and the C. elegans ortholog OSM-3 is autoinhibited through intramolecular tail–hinge folding relieved by cargo binding.

    Evidence Dominant-negative KIF17 in olfactory neurons (PMID:16782012); Co-IP of GluR6/KA2 with KIF17 and dominant-negative experiments (PMID:17174564); single-molecule motility, ATPase, and EM conformational analysis of OSM-3 (PMID:17000874)

    PMID:16782012 PMID:17000874 PMID:17174564

    Open questions at the time
    • Whether mammalian KIF17 uses the same hinge-based autoinhibition mechanism as OSM-3 not directly shown
    • Structural basis of autoinhibition at atomic resolution not yet available for mammalian KIF17
  7. 2007 High

    Identification of CaMKII phosphorylation at Ser1029 as the cargo-release trigger answered how NR2B is unloaded at the destination: phosphorylation disrupts the KIF17–Mint1 interaction, providing a signal-dependent delivery mechanism.

    Evidence FRET-based interaction assay, in vitro CaMKII kinase assay, site-directed mutagenesis, in vivo imaging

    PMID:18066053

    Open questions at the time
    • Whether other kinases phosphorylate Ser1029 in vivo unknown
    • Spatiotemporal dynamics of CaMKII activation relative to cargo unloading not resolved
  8. 2008 High

    Zebrafish Kif17 co-immunoprecipitated with IFT proteins and its knockdown disrupted photoreceptor outer segment formation, establishing KIF17 as an IFT-associated motor essential for vertebrate photoreceptor development.

    Evidence Co-IP, morpholino knockdown, immunofluorescence, and electron microscopy in zebrafish photoreceptors

    PMID:18304522

    Open questions at the time
    • Whether KIF17 is an essential anterograde IFT motor or accessory motor in mammalian photoreceptors debated
  9. 2010 High

    Discovery of a ciliary localization signal (CLS) in KIF17's tail homologous to NLSs, regulated by importin-β2 and a ciliary RanGTP gradient, revealed that ciliary entry uses a nuclear-import-like gating mechanism.

    Evidence CLS mutagenesis, importin-β2 Co-IP, dominant-negative RanG19V, live-cell fluorescence imaging

    PMID:20526328

    Open questions at the time
    • Whether the RanGTP gradient is the sole regulator of ciliary entry or whether Rab23 operates in parallel not resolved
  10. 2011 High

    The Kif17 knockout mouse confirmed essential in vivo roles: NR2B transport is blocked, NR2A is destabilized via proteasomal degradation, NMDAR-mediated plasticity (LTP/LTD) is impaired, and hippocampus-dependent memory is deficient.

    Evidence Kif17 germline knockout mouse with electrophysiology, behavior, ubiquitin-proteasome pathway analysis

    PMID:21521616

    Open questions at the time
    • Why NR2A is destabilized upon KIF17 loss (indirect mechanism) not fully elucidated
  11. 2012 High

    Phosphomimetic (S1029D) and phospho-null (S1029A) transgenic mice in the kif17−/− background both showed impaired NR2B delivery and memory deficits, demonstrating that CaMKII phosphocycling at Ser1029 is required for both cargo loading and unloading.

    Evidence Transgenic phosphomutant mice in kif17−/− background with electrophysiology and behavioral assays

    PMID:22514311

    Open questions at the time
    • Whether phosphocycling rate determines delivery efficiency not quantified
    • Upstream signals that time CaMKII activation for loading versus unloading remain unclear
  12. 2013 High

    Reconstitution studies showed that the KIF17 motor domain stabilizes MT plus-ends and inhibits depolymerization independently of EB1, while its tail domain binds tubulin dimers and delays polymerization, revealing a direct role in microtubule dynamics regulation beyond cargo transport.

    Evidence In vitro MT dynamics assays, ATPase assays, MT gliding, cell-based nocodazole resistance, domain competition experiments

    PMID:24072717

    Open questions at the time
    • Physiological relevance of KIF17-mediated MT stabilization in vivo not demonstrated
  13. 2015 High

    Rab23 was identified as a regulator of KIF17 ciliary entry, forming a complex with KIF17 and importin-β2, and both KIF17 and the IFT-B complex were shown to be required for D1 dopamine receptor delivery to the ciliary membrane.

    Evidence Co-IP, Rab23 depletion/mutant studies, receptor chimera assays, immunofluorescence

    PMID:26136363 PMID:26182404

    Open questions at the time
    • How Rab23 and RanGTP pathways are coordinated for KIF17 ciliary entry not resolved
    • Whether KIF17 directly transports the receptor or facilitates lateral membrane delivery unclear
  14. 2016 High

    Three parallel advances defined KIF17's mechanism in epithelial polarity and dendritic selectivity: KIF17 activates RhoA signaling at epithelial junctions through a motor-domain-dependent but microtubule-independent mechanism; SEPT9 competes with Mint1 for KIF17 tail binding to regulate NR2B transport; and dendritic targeting requires cargo-dependent autoinhibition relief, AIS actin filtering, and dynein-mediated redirection.

    Evidence RhoA pulldown, ROCK/LIMK inhibitors, 3D organotypic cultures (PMID:26759174); direct binding and Co-IP with NR2B transport assay (PMID:26823018); live-cell inducible trafficking, actin depolymerization, dynein inhibition in neurons (PMID:27265394)

    PMID:26759174 PMID:26823018 PMID:27265394

    Open questions at the time
    • How KIF17 motor domain activates RhoA without MT binding is mechanistically unclear
    • Whether SEPT9 regulation is activity-dependent not tested
  15. 2017 High

    Mapping the KIF17–IFT-B interface to IFT46–IFT56 and a C-terminal region upstream of the NLS clarified how KIF17 docks onto the IFT train, while showing KIF17 is dispensable for ciliogenesis and IFT-B intraciliary movement in mammalian cells.

    Evidence VIP assay, deletion/truncation mutants, Co-IP, fluorescence imaging of ciliary entry

    PMID:28077622

    Open questions at the time
    • Whether KIF17 provides anterograde force redundant with kinesin-2 heterotrimeric motor not resolved
    • Cargo specificity of KIF17 within cilia not comprehensively defined
  16. 2018 High

    CaMKII phosphorylation of KIF17 at the conserved Ser815/S1029 site was shown to promote outer segment localization and drive photoreceptor disc shedding, linking the same phosphoregulatory mechanism used for NR2B release in neurons to a distinct ciliary function.

    Evidence Phosphomimetic/phospho-null transgenic zebrafish, kif17 genetic mutants, disc shedding assays, constitutively active CaMKII expression

    PMID:30458707

    Open questions at the time
    • Whether disc shedding is a direct mechanical consequence of KIF17 motility or signaling-mediated unknown
  17. 2020 High

    Discovery that KIF17 is locally synthesized in dendrites in an NMDAR-activity-dependent manner via its 3′UTR, and that 3′UTR deletion impairs fear memory extinction, revealed a previously unrecognized layer of translational regulation governing motor availability at synapses.

    Evidence NMDAR agonist/antagonist, local synthesis assay, hippocampus-specific 3′UTR deletion mouse, fear memory behavioral assays

    PMID:33328231

    Open questions at the time
    • RNA-binding proteins mediating 3′UTR-dependent local translation not identified
    • Whether local KIF17 synthesis is specific to particular dendritic compartments unknown
  18. 2024 High

    Cell-type-specific Kif17 knockouts in cerebellum revealed dual opposing roles in Hedgehog signaling: KIF17 promotes SHH secretion in Purkinje cells while restraining GLI-dependent target gene expression in CGNPs, establishing KIF17 as a context-dependent Hedgehog pathway regulator.

    Evidence Germline and cell-type-specific conditional Kif17 knockout mice, HH target gene expression, SHH quantification, cerebellar morphometry

    PMID:38669326

    Open questions at the time
    • Mechanism by which KIF17 regulates GLI processing in CGNPs not molecularly defined
    • Whether these roles generalize to other Hedgehog-dependent tissues not tested

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structural basis of mammalian KIF17 autoinhibition, the identity of adaptors linking KIF17 to non-NR2B dendritic cargoes (e.g., Kv4.2), the precise force-generating role of KIF17 versus heterotrimeric kinesin-2 in mammalian IFT, and how the same CaMKII phosphorylation site coordinates cargo release across neuronal and ciliary contexts.
  • No high-resolution structure of full-length mammalian KIF17
  • Adaptor for Kv4.2 cargo unknown
  • Redundancy with heterotrimeric kinesin-2 in mammalian cilia not genetically resolved

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003774 cytoskeletal motor activity 4 GO:0140657 ATP-dependent activity 3 GO:0098772 molecular function regulator activity 2 GO:0008092 cytoskeletal protein binding 1
Localization
GO:0005929 cilium 6 GO:0031410 cytoplasmic vesicle 3 GO:0005856 cytoskeleton 2 GO:0005886 plasma membrane 1
Pathway
R-HSA-112316 Neuronal System 8 R-HSA-9609507 Protein localization 5 R-HSA-1852241 Organelle biogenesis and maintenance 3 R-HSA-382551 Transport of small molecules 3 R-HSA-162582 Signal Transduction 2
Partners
CAMK2AGRIN2BIFT46IFT56KPNB1MINT1RAB23SEPT9
Complex memberships
IFT-B complexmLin-2/mLin-7/mLin-10/NR2B transport complex

Evidence

Reading pass · 31 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 KIF17 directly interacts with the PDZ domain of mLin-10 (Mint1/X11) via its tail domain, linking KIF17 to a large protein complex (mLin-2/CASK, mLin-7/MALS/Velis, NR2B) and enabling transport of NR2B-containing vesicles along microtubules in neuronal dendrites. Co-immunoprecipitation, direct interaction pulldown, in vitro microtubule transport assay Science High 10846156
2003 KIF17 vesicles move processively along dendrites at ~0.76 μm/sec and are associated with extrasynaptic NR2B; knockdown or dominant-negative blockade of KIF17 significantly reduces NR2B expression and synaptic localization, with a compensatory increase in synaptic NR2A. Live-cell imaging in hippocampal neurons, dominant-negative expression, siRNA knockdown, immunostaining The Journal of Neuroscience High 12514209
2002 Overexpression of KIF17 in transgenic mice upregulates NR2B expression with increased CREB phosphorylation, enhancing spatial and working memory, establishing KIF17 as an in vivo regulator of NR2B trafficking and synaptic plasticity. Transgenic mouse overexpression, behavioral tasks, Western blotting, immunohistochemistry PNAS High 12391294
2007 CaMKII phosphorylates KIF17 at Ser1029 in its tail domain, disrupting the KIF17–Mint1 interaction and causing release of the NR2B cargo from microtubule-based transport, establishing a phosphorylation-dependent cargo release mechanism. FRET-based protein-protein interaction assay, in vitro kinase assay, site-directed mutagenesis, in vivo approaches Nature Cell Biology High 18066053
2010 KIF17 contains a ciliary localization signal (CLS) in its tail domain homologous to nuclear localization signals (NLSs); ciliary entry of KIF17 is regulated by importin-β2 binding (dependent on CLS and inhibited by RanGTP) and a ciliary-cytoplasmic RanGTP gradient, analogous to nuclear import. Deletion/mutagenesis of CLS, co-immunoprecipitation with importin-β2, dominant-negative RanG19V expression, live-cell fluorescence imaging Nature Cell Biology High 20526328
2006 KIF17 is required for ciliary targeting of olfactory CNG channels in mammalian olfactory sensory neurons; the CNGB1b subunit's RVxP motif is necessary for this KIF17-dependent transport into cilia. Dominant-negative KIF17 expression, immunofluorescence, subcellular fractionation in olfactory epithelium Current Biology High 16782012
2008 In zebrafish photoreceptors, Kif17 co-localizes with IFT proteins within the outer segment (OS), co-immunoprecipitates with IFT proteins, and knockdown of Kif17 severely disrupts OS formation and visual pigment targeting, establishing Kif17 as essential for photoreceptor OS development via IFT. Co-immunoprecipitation, morpholino knockdown in zebrafish, immunofluorescence, electron microscopy Developmental Biology High 18304522
2011 In kif17-/- mice, NR2B transport is inhibited and nr2b transcription is decreased; NR2A levels are also reduced through accelerated ubiquitin-proteasome-dependent degradation. NMDAR-mediated synaptic currents, LTP, LTD, and CREB responses are attenuated, causing hippocampus-dependent memory impairment. Kif17 knockout mouse, electrophysiology, behavioral tests, Western blotting, ubiquitin-proteasome pathway analysis Neuron High 21521616
2012 Phosphorylation of KIF17 at Ser1029 (by CaMKII) controls both loading and unloading of GluN2B cargo in vivo; phosphomimetic (S1029D) and phospho-null (S1029A) transgenic mice in kif17-/- background both show reduced synaptic NMDA receptors, impaired plasticity, and spatial memory deficits due to inability to properly load/unload GluN2B. Transgenic phosphomutant mice in kif17-/- background, electrophysiology, behavioral tests, immunostaining The Journal of Neuroscience High 22514311
2005 KIF17 transports the dendritic K+ channel Kv4.2 to dendrites; dominant-negative KIF17 inhibits Kv4.2 dendritic localization, Kv4.2 co-immunoprecipitates with KIF17 from brain lysate, and the interaction is mediated through the extreme C-terminus of Kv4.2. Dominant-negative expression, co-immunoprecipitation from brain and COS cells, immunofluorescence in cortical neurons The Journal of Biological Chemistry High 16257958
2006 KIF17 interacts with kainate receptor subunits GluR6 and KA2, and is required for localization of GluR5-containing kainate receptors to distal dendrites of hippocampal neurons, establishing KIF17 as a motor for this receptor subtype. Co-immunoprecipitation, dominant-negative KIF17 expression, immunofluorescence in hippocampal neurons Molecular and Cellular Neurosciences Medium 17174564
2006 The testis-specific KIF17b physically interacts with MIWI (a PIWI/Argonaute family member) in chromatoid bodies of round spermatids; KIF17b localizes in chromatoid bodies and its presence offers a mechanism for microtubule-dependent chromatoid body mobility and transport of RNA components. Co-immunoprecipitation, immunofluorescence, live imaging of chromatoid body movement Journal of Cell Science Medium 16787948
2003 KIF17b co-immunoprecipitates with TB-RBP (Translin) in an RNA-protein complex containing specific CREM-regulated mRNAs; KIF17b functions as a motor component of an mRNP complex transporting CREM-regulated mRNAs in male postmeiotic germ cells, with TB-RBP release coinciding with the time of mRNA translation. Co-immunoprecipitation, in vivo RNA-protein crosslinking, in situ hybridization combined with immunohistochemistry at EM level, transfection assays PNAS Medium 14673085
2005 KIF17b's nucleocytoplasmic shuttling and transport of the transcriptional coactivator ACT (activator of CREM in testis) are independent of its motor domain and microtubules; instead, protein kinase A phosphorylates KIF17b and regulates its subcellular localization. Motor domain deletion mutants, microtubule depolymerization assays, PKA inhibition, immunofluorescence The Journal of Biological Chemistry Medium 16002395
2013 The KIF17 motor domain (K370) directly stabilizes microtubule plus-ends and inhibits depolymerization independently of EB1, while the KIF17 autoinhibitory tail domain binds MTs and tubulin dimers, delaying MT polymerization; EB1 and KIF17-Tail competitively modulate K370 ATPase activity. In vitro MT dynamic instability assays, in vitro ATPase assays, MT gliding assay, cell-based nocodazole depolymerization assay, domain truncation/competition experiments The Journal of Biological Chemistry High 24072717
2016 KIF17 localizes at cell-cell adhesions and its motor domain (independent of microtubule binding) activates RhoA-GTPase signaling via RhoA→ROCK→LIMK→cofilin pathway to drive junctional actin remodeling and stabilize the apical junctional complex in epithelial cells. KIF17 KD and OE with truncation mutants, active RhoA pulldown assay, ROCK/LIMK inhibitors, actin incorporation assay, 3D organotypic cultures Journal of Cell Science Medium 26759174
2016 Polarized dendritic targeting of KIF17 operates via three steps: (1) cargo binding relieves KIF17 autoinhibition and initiates transport; (2) KIF17-vesicles enter axons but are excluded by the actin cytoskeleton at the axon initial segment (AIS); (3) dynein activity redirects KIF17-coupled cargoes into dendrites. Live-cell imaging with inducible trafficking assays, actin depolymerization at AIS, dynein inhibition, dominant-negative constructs in neurons Current Biology High 27265394
2016 Septin 9 (SEPT9) directly associates with the C-terminal tail of KIF17 (the cargo-binding domain), preferentially in the extended conformation, and competitively interferes with mLin-10/Mint1 PDZ1 binding to KIF17, thereby downregulating NR2B transport into dendrites without affecting KIF17 microtubule-dependent motility. Direct binding assay (in vitro), co-immunoprecipitation, live-cell imaging of KIF17 motility, NR2B transport assay in hippocampal neurons, domain mapping Molecular Biology of the Cell High 26823018
2017 KIF17 interacts with the IFT46-IFT56 dimer of the IFT-B complex through its C-terminal sequence immediately upstream of the NLS; ciliary entry of KIF17 requires both IFT-B binding and its NLS (which binds importin-α), but KIF17 is dispensable for ciliogenesis and IFT-B intraciliary trafficking in mammalian cells. Visible immunoprecipitation (VIP) assay, deletion/truncation mutants, co-immunoprecipitation, fluorescence imaging Molecular Biology of the Cell High 28077622
2015 D1-type dopamine receptors are delivered to the ciliary membrane from the extra-ciliary plasma membrane via a mechanism requiring IFT-B complex and KIF17; Rab23 (a small GTPase) is essential for this process, as Rab23 depletion prevents receptor ciliary access and Rab23 fusion to a non-ciliary receptor drives its ciliary targeting. siRNA depletion of IFT-B/KIF17/Rab23, receptor chimeras, fluorescence imaging, nucleotide-dependent Rab23 fusion constructs eLife High 26182404
2015 Rab23 exists in a complex with KIF17 and importin-β2, and Rab23 depletion disrupts ciliary localization of KIF17; active Rab23 (Q68L) localizes to cilia and promotes KIF17 ciliary targeting through a mechanism distinct from Ran-GTP. Co-immunoprecipitation, affinity-binding studies, siRNA depletion, immunofluorescence, constitutively active/inactive Rab23 mutants Journal of Cell Science Medium 26136363
2018 CaMKII-mediated phosphorylation of KIF17 at S815 (zebrafish)/S1029 (mouse) promotes its localization along the cone outer segment and drives photoreceptor outer segment disc shedding in a cell-autonomous manner; constitutively active tCaMKII increases disc shedding in a kif17-dependent manner. Transgenic zebrafish with phosphomimetic/phospho-null KIF17, genetic kif17 mutants, disc shedding assay, constitutively active CaMKII expression BMC Cell Biology High 30458707
2019 GTP-bound Rab23 recruits Kif17 to meiotic spindle poles in mouse oocytes; Kif17 regulates tubulin acetylation by associating with αTAT and Sirt2, and its tail domain interacts with the RhoA-ROCK-LIMK-cofilin pathway to modulate actin filament assembly needed for spindle migration to the cortex during meiosis. siRNA depletion of Rab23/Kif17, co-immunoprecipitation of tail domain with pathway components, immunofluorescence, polar body extrusion assays in mouse oocytes Development Medium 30696709
2020 KIF17 is locally degraded and synthesized in an NMDAR-mediated activity-dependent manner in individual dendrites; local KIF17 synthesis is driven by its 3'UTR, and hippocampus-specific deletion of Kif17 3'UTR impairs fear memory extinction by disrupting activity-dependent local KIF17 synthesis. NMDAR antagonist/agonist treatment, dendritic local synthesis assay, 3'UTR deletion mouse model, fear memory behavioral assays, fluorescence imaging Science Advances High 33328231
2017 KIF17 interacts with the orphan nuclear receptor ERR1 (ERRα) via its C-terminal tail domain (through an LXXLL nuclear receptor box motif); KIF17 tail expression attenuates nuclear accumulation of ERR1 and inhibits its transcriptional activity, while KIF17 knockout elevates ERR1 activity. Yeast-2-hybrid screen, in vitro biochemical binding assay, domain mapping, ERR1 transcriptional reporter assay, KIF17 KO cells, nuclear localization imaging Oncotarget Medium 28881568
2013 Mouse TTC30B (ortholog of C. elegans DYF-1) interacts with KIF17 and multiple IFT-B complex proteins, suggesting TTC30B links KIF17 to the IFT-B complex for ciliary transport. Co-immunoprecipitation from pituitary cells, mass spectrometry, cell-free co-immunoprecipitation Experimental Cell Research Medium 23810713
2006 OSM-3 (C. elegans KIF17 ortholog) is autoinhibited in solution through an intramolecular interaction involving folding at a central hinge; a point mutation (G444E) in the hinge or hinge deletion activates ATPase activity and processive movement, and cargo attachment to beads also relieves autoinhibition. Single-molecule fluorescence motility assay, MT-stimulated ATPase assay, point mutations, optical trap, conformation analysis by electron microscopy The Journal of Cell Biology High 17000874
2021 Crystal structures of the OSM-3 (KIF17 ortholog) motor domain in ADP-bound, nucleotide-free, and ATP-analog-bound states show that OSM-3 shares an identical nucleotide-binding site with kinesin-5 Eg5, supporting a universal two-water ATP hydrolysis mechanism in the kinesin superfamily including kinesin-2. X-ray crystallography of OSM-3 motor domain in multiple nucleotide states, structural comparison FEBS Open Bio High 33513284
2024 KIF17 has dual opposing roles in HH-dependent cerebellar development: in Purkinje cells (HH-producing), KIF17 deletion reduces SHH protein levels and decreases CGNP proliferation; in CGNPs (HH-responding), KIF17 deletion increases HH target gene expression and CGNP proliferation through altered GLI transcription factor processing. Germline and cell-type-specific conditional Kif17 knockout mice, HH pathway target gene expression analysis, SHH protein quantification, cerebellar morphometry Science Advances High 38669326
2022 KIF17 is modified by SUMOylation, which contributes to its stabilization and maintenance in epileptic neurons; KIF17 overexpression increases NR2B membrane expression and excitatory synaptic transmission, whereas knockdown has the opposite effect. Kainic acid epilepsy mouse model, KIF17 OE/KD, electrophysiology, SUMO modification assay, Western blotting Neuroscience Bulletin Medium 35678994
2006 Spatial-ε protein co-localizes and co-immunoprecipitates with KIF17b in spermatid manchette and sperm tail principal piece, establishing Spatial-ε as a KIF17b cargo during spermatogenesis. Co-immunoprecipitation from testis lysates, indirect immunofluorescence co-localization Experimental Cell Research Medium 17196196

Source papers

Stage 0 corpus · 61 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 Kinesin superfamily motor protein KIF17 and mLin-10 in NMDA receptor-containing vesicle transport. Science (New York, N.Y.) 584 10846156
2010 Ciliary entry of the kinesin-2 motor KIF17 is regulated by importin-beta2 and RanGTP. Nature cell biology 223 20526328
2006 Ciliary targeting of olfactory CNG channels requires the CNGB1b subunit and the kinesin-2 motor protein, KIF17. Current biology : CB 175 16782012
2003 KIF17 dynamics and regulation of NR2B trafficking in hippocampal neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience 168 12514209
2006 Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors. The Journal of cell biology 158 17000880
2007 Disruption of KIF17-Mint1 interaction by CaMKII-dependent phosphorylation: a molecular model of kinesin-cargo release. Nature cell biology 154 18066053
2002 Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice. Proceedings of the National Academy of Sciences of the United States of America 147 12391294
2011 Molecular motor KIF17 is fundamental for memory and learning via differential support of synaptic NR2A/2B levels. Neuron 117 21521616
2008 The homodimeric kinesin, Kif17, is essential for vertebrate photoreceptor sensory outer segment development. Developmental biology 116 18304522
1995 Exclusive expression of C. elegans osm-3 kinesin gene in chemosensory neurons open to the external environment. Journal of molecular biology 103 7714894
2006 Interplay of PIWI/Argonaute protein MIWI and kinesin KIF17b in chromatoid bodies of male germ cells. Journal of cell science 102 16787948
1993 C. elegans osm-3 gene mediating osmotic avoidance behaviour encodes a kinesin-like protein. Neuroreport 102 7690265
2006 Autoinhibition regulates the motility of the C. elegans intraflagellar transport motor OSM-3. The Journal of cell biology 91 17000874
2005 A role for Kif17 in transport of Kv4.2. The Journal of biological chemistry 85 16257958
2015 Dopamine receptors reveal an essential role of IFT-B, KIF17, and Rab23 in delivering specific receptors to primary cilia. eLife 70 26182404
2003 The kinesin KIF17b and RNA-binding protein TB-RBP transport specific cAMP-responsive element modulator-regulated mRNAs in male germ cells. Proceedings of the National Academy of Sciences of the United States of America 69 14673085
2017 Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal. Molecular biology of the cell 50 28077622
2012 Regulation of NMDA receptor transport: a KIF17-cargo binding/releasing underlies synaptic plasticity and memory in vivo. The Journal of neuroscience : the official journal of the Society for Neuroscience 49 22514311
2009 Different roles for KIF17 and kinesin II in photoreceptor development and maintenance. Developmental dynamics : an official publication of the American Association of Anatomists 49 19384852
2006 Localization of glutamate receptors to distal dendrites depends on subunit composition and the kinesin motor protein KIF17. Molecular and cellular neurosciences 46 17174564
2015 A role for Rab23 in the trafficking of Kif17 to the primary cilium. Journal of cell science 38 26136363
2006 Dynamic distribution of Spatial during mouse spermatogenesis and its interaction with the kinesin KIF17b. Experimental cell research 36 17196196
2005 Microtubule-independent and protein kinase A-mediated function of kinesin KIF17b controls the intracellular transport of activator of CREM in testis (ACT). The Journal of biological chemistry 36 16002395
2012 The kinesin superfamily protein KIF17: one protein with many functions. Biomolecular concepts 35 23762210
2019 Rab23/Kif17 regulate meiotic progression in oocytes by modulating tubulin acetylation and actin dynamics. Development (Cambridge, England) 34 30696709
2016 Septin 9 interacts with kinesin KIF17 and interferes with the mechanism of NMDA receptor cargo binding and transport. Molecular biology of the cell 30 26823018
2018 Kif17 phosphorylation regulates photoreceptor outer segment turnover. BMC cell biology 27 30458707
2014 The inhibitor of calcium/calmodulin-dependent protein kinase II KN93 attenuates bone cancer pain via inhibition of KIF17/NR2B trafficking in mice. Pharmacology, biochemistry, and behavior 27 24836181
2022 Modulation of KIF17/NR2B crosstalk by tozasertib attenuates inflammatory pain in rats. Inflammopharmacology 24 35243557
2016 Three-Step Model for Polarized Sorting of KIF17 into Dendrites. Current biology : CB 24 27265394
2012 Prenatal stress disturbs hippocampal KIF17 and NR2B in spatial cognition in male offspring. Journal of neuroscience research 23 23239421
2022 Inhibition of pan-Aurora kinase attenuates evoked and ongoing pain in nerve injured rats via regulating KIF17-NR2B mediated signaling. International immunopharmacology 22 35183034
2017 Cos2/Kif7 and Osm-3/Kif17 regulate onset of outer segment development in zebrafish photoreceptors through distinct mechanisms. Developmental biology 19 28341548
2010 The kinesin superfamily protein KIF17 is regulated by the same transcription factor (NRF-1) as its cargo NR2B in neurons. Biochimica et biophysica acta 18 21172391
2016 A Screen for Modifiers of Cilia Phenotypes Reveals Novel MKS Alleles and Uncovers a Specific Genetic Interaction between osm-3 and nphp-4. PLoS genetics 16 26863025
2013 Direct regulation of microtubule dynamics by KIF17 motor and tail domains. The Journal of biological chemistry 16 24072717
2012 Analysis of KIF17 distal tip trafficking in zebrafish cone photoreceptors. Vision research 16 23099049
2009 The presence of kinesin superfamily motor proteins KIFC1 and KIF17 in normal and pathological human placenta. Placenta 16 19679349
2008 Altered expression of KIF17, a kinesin motor protein associated with NR2B trafficking, may mediate learning deficits in a Down syndrome mouse model. American journal of obstetrics and gynecology 15 18313453
2016 KIF17 regulates RhoA-dependent actin remodeling at epithelial cell-cell adhesions. Journal of cell science 14 26759174
2014 Intrathecal injection of selected peptide Myr-RC-13 attenuates bone cancer pain by inhibiting KIF17 and NR2B expression. Pharmacology, biochemistry, and behavior 13 24726705
2013 Interaction of mouse TTC30/DYF-1 with multiple intraflagellar transport complex B proteins and KIF17. Experimental cell research 12 23810713
2022 KIF17 Modulates Epileptic Seizures and Membrane Expression of the NMDA Receptor Subunit NR2B. Neuroscience bulletin 11 35678994
2020 An activity-dependent local transport regulation via degradation and synthesis of KIF17 underlying cognitive flexibility. Science advances 11 33328231
2018 KIF17 mediates the learning and memory impairment in offspring induced by maternal exposure to propofol during middle pregnancy. Molecular medicine reports 9 29393422
2007 Neuronal distribution of spatial in the developing cerebellum and hippocampus and its somatodendritic association with the kinesin motor KIF17. Experimental cell research 9 17961552
2022 Betaine Attenuates Chronic Constriction Injury-Induced Neuropathic Pain in Rats by Inhibiting KIF17-Mediated Nociception. ACS chemical neuroscience 7 36367842
2014 The cyclic AMP response element-binding protein antisense oligonucleotide induced anti-nociception and decreased the expression of KIF17 in spinal cord after peripheral nerve injury in mice. International journal of clinical and experimental medicine 7 25664020
2023 Sida cordifolia L. attenuates behavioral hypersensitivity by interfering with KIF17-NR2B signaling in rat model of neuropathic pain. Journal of ethnopharmacology 6 37640257
2021 R869C mutation in molecular motor KIF17 gene is involved in dementia with Lewy bodies. Alzheimer's & dementia (Amsterdam, Netherlands) 6 34124335
2017 Modulation of estrogen related receptor alpha activity by the kinesin KIF17. Oncotarget 6 28881568
2015 A decrease in protein level and a missense polymorphism of KIF17 are associated with schizophrenia. Psychiatry research 6 26421900
2000 C. elegans KLP-11/OSM-3/KAP-1: orthologs of the sea urchin kinesin-II, and mouse KIF3A/KIFB/KAP3 kinesin complexes. DNA research : an international journal for rapid publication of reports on genes and genomes 6 10819327
2021 Biallelic Variants in KIF17 Associated with Microphthalmia and Coloboma Spectrum. International journal of molecular sciences 5 33922911
2022 The potential function of KIF17 in large yellow croaker (Larimichthys crocea) spermatid remodeling: molecular characterization and expression pattern during spermiogenesis. Fish physiology and biochemistry 4 35538183
2021 Structural snapshots of the kinesin-2 OSM-3 along its nucleotide cycle: implications for the ATP hydrolysis mechanism. FEBS open bio 4 33513284
2024 Dual and opposing roles for the kinesin-2 motor, KIF17, in Hedgehog-dependent cerebellar development. Science advances 3 38669326
2025 Phosphorylation-dependent regional motility of the ciliary kinesin OSM-3. The Journal of cell biology 2 40272473
2023 Expression Dynamics Indicate Potential Roles of KIF17 for Nuclear Reshaping and Tail Formation during Spermiogenesis in Phascolosoma esculenta. International journal of molecular sciences 2 38203305
2022 KIF17 maintains the epithelial phenotype of breast cancer cells and curbs tumour metastasis. Cancer letters 2 36089118
2025 GRIN2B alleviates mid-gestational sevoflurane exposure-induced early differentiation of rat neural stem cells by interacting with KIF17. Journal of cell communication and signaling 1 40568341