Affinage

KIF17

Kinesin-like protein KIF17 · UniProt Q9P2E2

Audit flag: ungrounded claim
Length
1029 aa
Mass
115.1 kDa
Annotated
2026-06-10
61 papers in source corpus 34 papers cited in narrative 34 extracted findings
Cross-family judge vs UniProt: tie faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

KIF17 is a homodimeric kinesin-2 motor with two principal cellular roles: microtubule-based cargo transport into neuronal dendrites and intraflagellar transport within cilia (PMID:10846156, PMID:20526328). In neurons, KIF17 links to NR2B-containing NMDA receptor vesicles through a direct interaction between its tail domain and the PDZ domain of mLin-10/Mint1 within a larger Mint1/CASK/MALS scaffold, and moves these vesicles processively along dendrites (PMID:10846156, PMID:12514209); loss of KIF17 reduces NR2B transport, transcription, synaptic NMDAR currents, LTP/LTD, and hippocampus-dependent memory (PMID:21521616). Cargo loading and release are gated by CaMKII phosphorylation of the tail at Ser1029, which disrupts the KIF17–Mint1 interaction, and both phospho-null and phospho-mimetic states impair synaptic plasticity, establishing that bidirectional phospho-cycling is required for productive transport (PMID:18066053, PMID:22514311). KIF17 also transports the Kv4.2 K+ channel and kainate receptor subunits to dendrites (PMID:16257958, PMID:17174564), and its abundance is controlled by NRF-1-driven transcription co-regulated with NR2B and by activity-dependent local dendritic synthesis directed by its 3'UTR (PMID:21171239, PMID:33328231). Motor output is regulated by autoinhibitory folding that is relieved by cargo binding, while polarized dendritic delivery additionally requires an actin filter at the axon initial segment and dynein-based redirection (PMID:17000874, PMID:27265394). In cilia, KIF17 enters via a tail ciliary localization signal recognized by importins under control of a Ran-GTP gradient and by direct binding to the IFT46–IFT56 dimer of the IFT-B complex, functioning in intraflagellar transport to build photoreceptor outer segments and deliver ciliary membrane receptors (PMID:20526328, PMID:28077622, PMID:18304522, PMID:26182404); the C. elegans ortholog OSM-3 cooperates mechanically with kinesin-II and is activated specifically in the distal ciliary segment through NEKL kinase/PP2A control of its motor domain (PMID:17000880, PMID:40272473). Beyond transport, the KIF17 motor domain directly stabilizes microtubule plus-ends and activates RhoA–ROCK–LIMK–cofilin signaling to reinforce epithelial junctions, and the tail modulates ERRα and Hedgehog/GLI-dependent transcriptional programs in development (PMID:24072717, PMID:26759174, PMID:28881568, PMID:38669326).

Mechanistic history

Synthesis pass · year-by-year structured walk · 33 steps
  1. 2000 High

    Established how a kinesin selects a specific dendritic cargo, by showing KIF17 binds the Mint1 PDZ scaffold to transport NR2B vesicles.

    Evidence Co-IP and direct tail–PDZ binding assay with vesicle transport in neurons

    PMID:10846156

    Open questions at the time
    • Did not define how cargo binding is regulated
    • Did not address polarized dendrite-versus-axon sorting
  2. 2002 High

    Demonstrated in vivo that KIF17 motor activity drives NR2B transport and downstream CREB-linked transcription relevant to memory.

    Evidence Transgenic GFP-KIF17 overexpression mice with behavioral and biochemical readouts

    PMID:12391294

    Open questions at the time
    • Overexpression may not reflect endogenous stoichiometry
    • Did not establish loss-of-function requirement
  3. 2003 High

    Quantified KIF17 dendritic motility and showed its requirement for NR2B synaptic delivery with compensatory NR2A changes.

    Evidence Live imaging, siRNA knockdown and dominant-negative in hippocampal neurons

    PMID:12514209

    Open questions at the time
    • Did not define the unloading signal at synapses
  4. 2007 High

    Identified the molecular switch for cargo release: CaMKII phosphorylation of Ser1029 disrupts the KIF17–Mint1 interaction.

    Evidence In vitro kinase assay, FRET, mutagenesis and Co-IP

    PMID:18066053

    Open questions at the time
    • In vitro/cell-based; in vivo physiological role established only later
  5. 2011 High

    A clean knockout confirmed KIF17 controls NR2B transport and transcription plus NR2A stability, and is required for plasticity and memory.

    Evidence Kif17 knockout mice with electrophysiology, behavior, and proteasome rescue

    PMID:21521616

    Open questions at the time
    • Mechanism coupling transport to nr2b transcription not resolved
  6. 2012 High

    Showed Ser1029 phospho-cycling is bidirectionally essential in vivo: phospho-null blocks loading, phospho-mimetic blocks unloading.

    Evidence S1029A/S1029D knock-in mice in kif17-null background

    PMID:22514311

    Open questions at the time
    • Spatial cues controlling where CaMKII acts not defined
  7. 2005 Medium

    Extended KIF17 cargo repertoire to the Kv4.2 K+ channel, broadening its role in dendritic membrane protein delivery.

    Evidence Dominant-negative constructs and Co-IP from brain and COS cells

    PMID:16257958

    Open questions at the time
    • Direct versus indirect interaction not resolved
    • No reconstitution
  8. 2006 Medium

    Added kainate receptor subunits as KIF17 cargo, further generalizing its dendritic glutamate-receptor transport function.

    Evidence Co-IP from hippocampal neurons and dominant-negative KIF17

    PMID:17174564

    Open questions at the time
    • Adaptor linking KIF17 to kainate receptors unknown
    • Single lab
  9. 2003 Medium

    Defined a germline transport role: testis isoform KIF17b moves CREM-regulated mRNPs in complex with TB-RBP.

    Evidence Co-IP, RNA crosslinking and EM-level in situ hybridization in male germ cells

    PMID:14673085

    Open questions at the time
    • mRNA selectivity determinants unknown
    • Single lab
  10. 2005 Medium

    Revealed a non-canonical, motor-independent KIF17b function: PKA-regulated nucleocytoplasmic shuttling of the CREM coactivator ACT.

    Evidence Truncations, nocodazole, PKA modulation and fractionation

    PMID:16002395

    Open questions at the time
    • Mechanism of microtubule-independent transport undefined
    • Single lab
  11. 2006 Medium

    Connected KIF17b to germ-cell RNA granules through interaction with the PIWI protein MIWI in chromatoid bodies.

    Evidence Co-IP, localization, and miwi-null spermatid analysis

    PMID:16787948

    Open questions at the time
    • Functional consequence of MIWI association not quantified
  12. 2006 Medium

    First placed the mammalian motor in ciliary transport, showing KIF17 is needed to deliver olfactory CNG channels to cilia.

    Evidence Dominant-negative KIF17 in olfactory sensory neurons

    PMID:16782012

    Open questions at the time
    • Dominant-negative only; no endogenous knockout
    • Direct cargo interaction unmapped
  13. 2006 High

    Reconstituted the core ciliary mechanism in C. elegans: OSM-3 and kinesin-II cooperate by mechanical competition to drive IFT.

    Evidence In vitro gliding with purified motors plus in vivo IFT in mutants

    PMID:17000880

    Open questions at the time
    • Generalization to mammalian KIF17 not directly tested here
  14. 2006 High

    Defined the autoregulatory logic of the motor: OSM-3 is autoinhibited by hinge folding and activated by cargo or hinge mutation.

    Evidence Single-molecule, ATPase, optical trap and G444E mutagenesis

    PMID:17000874

    Open questions at the time
    • Physiological activating cargo in vivo not identified here
  15. 2008 High

    Showed vertebrate KIF17 is part of the photoreceptor IFT machinery and required for outer segment formation.

    Evidence Zebrafish morpholino knockdown and Co-IP with IFT proteins

    PMID:18304522

    Open questions at the time
    • Specific IFT subunit contacts not mapped here
  16. 2010 High

    Identified a ciliary localization signal in the tail recognized by importin-β2 under Ran-GTP control, paralleling nuclear import.

    Evidence CLS fusion/deletion, importin-β2 Co-IP, dominant-negative RanG19V imaging

    PMID:20526328

    Open questions at the time
    • Did not show direct IFT-complex binding (resolved 2017)
  17. 2010 High

    Linked KIF17 transcription to neuronal activity by identifying NRF-1 as a direct promoter regulator co-regulating KIF17 and NR2B.

    Evidence EMSA, ChIP, promoter mutants, NRF-1 knockdown/overexpression

    PMID:21171239

    Open questions at the time
    • Other transcriptional inputs not excluded
  18. 2013 High

    Showed the motor doubles as a microtubule plus-end stabilizer competing with EB1, separating its enzymatic and lattice-regulating activities.

    Evidence In vitro dynamic instability, ATPase, cosedimentation with purified domains

    PMID:24072717

    Open questions at the time
    • In-cell physiological relevance of plus-end stabilization not fully defined
  19. 2013 Medium

    Added a candidate IFT-linking adaptor by showing TTC30B/DYF-1 directly binds KIF17 and associates with IFT-B.

    Evidence Co-IP from pituitary cells and cell-free expression Co-IP with MS

    PMID:23810713

    Open questions at the time
    • Functional consequence of the interaction untested
    • Single lab
  20. 2015 High

    Defined a Rab23-dependent route for ciliary membrane receptor delivery requiring IFT-B and KIF17.

    Evidence siRNA of IFT-B/KIF17/Rab23 plus Rab23 fusion mis-targeting, live imaging

    PMID:26182404

    Open questions at the time
    • Direct KIF17–receptor contact not established
  21. 2015 Medium

    Placed Rab23 upstream of KIF17 ciliary entry as part of a Rab23–KIF17–importin-β2 complex.

    Evidence Co-IP, affinity binding, siRNA knockdown and immunofluorescence

    PMID:26136363

    Open questions at the time
    • Whether Rab23 directly contacts KIF17 versus via importin unresolved
    • Single lab
  22. 2016 High

    Resolved the dendrite-targeting logic into three steps: cargo-activated transport, an AIS actin filter, and dynein-mediated redirection.

    Evidence Inducible cargo trafficking, actin depolymerization and dynein inhibition imaging

    PMID:27265394

    Open questions at the time
    • Molecular identity of the AIS filtering machinery unspecified
  23. 2016 High

    Identified SEPT9 as a negative regulator competing with Mint1 for the KIF17 tail to throttle NR2B transport.

    Evidence GST pulldown with purified proteins, Co-IP, live motility and NR2B imaging

    PMID:26823018

    Open questions at the time
    • Upstream control of SEPT9–KIF17 competition unknown
  24. 2016 High

    Uncovered a transport-independent epithelial role: KIF17 motor domain activates RhoA–ROCK–LIMK–cofilin to reinforce junctions.

    Evidence siRNA/OE, pathway inhibitors and activated mutants, 3D organotypic culture

    PMID:26759174

    Open questions at the time
    • How the motor domain engages RhoA signaling biochemically not defined
  25. 2017 High

    Mapped a second ciliary-entry determinant: direct KIF17 binding to the IFT46–IFT56 dimer, independent of the NLS and motor domain.

    Evidence Visible immunoprecipitation, deletion mutants, importin-α Co-IP, tip imaging

    PMID:28077622

    Open questions at the time
    • Relative contribution of the two determinants in different cilia unresolved
  26. 2017 Medium

    Extended the tail's regulatory reach to transcription, showing an LXXLL motif binds ERRα and restrains its nuclear accumulation and activity.

    Evidence Yeast-2-hybrid, in vitro binding, KO cells, fractionation and reporter assays

    PMID:28881568

    Open questions at the time
    • Physiological context of ERRα regulation unclear
    • Single lab
  27. 2018 High

    Showed the conserved CaMKII/Ser phospho-switch also governs ciliary KIF17 by driving photoreceptor disc shedding.

    Evidence Phospho-mimetic/null zebrafish, kif17 mutants in two species, CaMKII epistasis

    PMID:30458707

    Open questions at the time
    • Mechanism linking KIF17 localization to disc shedding undefined
  28. 2019 Medium

    Implicated KIF17 in meiotic spindle migration downstream of GTP-Rab23 and via RhoA-pathway components in oocytes.

    Evidence siRNA of Rab23/Kif17, spindle imaging, αTAT/Sirt2 blots and Co-IP

    PMID:30696709

    Open questions at the time
    • Direct versus indirect RhoA-pathway engagement unresolved
    • Single lab
  29. 2020 High

    Revealed activity-dependent local control of KIF17 abundance through 3'UTR-driven dendritic synthesis required for fear memory extinction.

    Evidence Live imaging of synthesis, 3'UTR reporters, NMDAR modulation, conditional 3'UTR deletion mice with behavior

    PMID:33328231

    Open questions at the time
    • RNA-binding factors directing local synthesis not identified
  30. 2021 High

    Provided structural basis for the conserved kinesin-2 ATP hydrolysis mechanism via crystallized OSM-3 motor states.

    Evidence X-ray structures along the nucleotide cycle compared with Eg5

    PMID:33513284

    Open questions at the time
    • Structures of cargo-bound or autoinhibited full-length motor lacking
  31. 2022 Medium

    Linked KIF17 stability to disease by showing SUMOylation maintains KIF17 levels and modulates epileptic NR2B membrane expression.

    Evidence KD/OE in epilepsy model, SUMO assay, electrophysiology, NR2B fractionation

    PMID:35678994

    Open questions at the time
    • SUMO sites and ligases not mapped
    • Single lab
  32. 2024 High

    Demonstrated opposing cell-type-specific roles for KIF17 in Hedgehog-driven cerebellar development through SHH levels and GLI processing.

    Evidence Germline and cell-type-specific Kif17 deletion mice with HH pathway and GLI readouts

    PMID:38669326

    Open questions at the time
    • Molecular mechanism by which KIF17 influences GLI processing undefined
  33. 2025 High

    Defined spatial control of motor activation: NEKL-3 phosphorylates the OSM-3 motor domain and NEKL/PP2A restrict activation to the distal cilium.

    Evidence In vitro kinase assay, in vivo FLIM conformational imaging, NEKL/PP2A genetics, FRAP

    PMID:40272473

    Open questions at the time
    • Conservation of NEKL regulation for mammalian KIF17 untested

Open questions

Synthesis pass · forward-looking unresolved questions
  • How KIF17's diverse tail-mediated functions—cargo transport, transcriptional modulation, junctional signaling, and ciliary entry—are coordinated and selected within a single cell remains unresolved.
  • No structure of cargo-bound full-length mammalian KIF17
  • Mechanism switching between motor-dependent and motor-independent functions undefined
  • Direct disease-causing human mutations not established in this corpus

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003774 cytoskeletal motor activity 4 GO:0140657 ATP-dependent activity 4 GO:0060090 molecular adaptor activity 3 GO:0140110 transcription regulator activity 2 GO:0008092 cytoskeletal protein binding 1
Localization
GO:0005929 cilium 5 GO:0005829 cytosol 3 GO:0005856 cytoskeleton 3 GO:0005634 nucleus 2 GO:0005815 microtubule organizing center 1 GO:0005886 plasma membrane 1
Pathway
R-HSA-112316 Neuronal System 4 R-HSA-1852241 Organelle biogenesis and maintenance 4 R-HSA-9609507 Protein localization 4 R-HSA-1266738 Developmental Biology 2 R-HSA-162582 Signal Transduction 2
Partners
ESRRAIFT46IFT56KPNB1MINT1RAB23SEPT9TTC30B
Complex memberships
IFT-B complexKIF17b–TB-RBP mRNPmLin-10/Mint1–CASK–MALS scaffold

Evidence

Reading pass · 34 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 KIF17 directly interacts with the PDZ domain of mLin-10 (Mint1/X11) via its tail domain, linking it to a large protein complex (mLin-2/CASK, mLin-7/MALS/Velis, NR2B) to transport NR2B-containing vesicles along microtubules in neuronal dendrites. Co-immunoprecipitation, direct binding assay (tail-PDZ interaction), vesicle transport reconstitution in neurons Science High 10846156
2003 KIF17 vesicles move processively along dendrites at ~0.76 µm/sec and are associated with extrasynaptic NR2B; knockdown or dominant-negative blockade of KIF17 reduces NR2B expression and its synaptic localization, accompanied by a compensatory increase in synaptic NR2A. Live-cell imaging of GFP-tagged KIF17 in hippocampal neurons, siRNA knockdown, dominant-negative overexpression, immunostaining The Journal of Neuroscience High 12514209
2002 Overexpression of KIF17 in transgenic mice upregulates NR2B expression and increases CREB phosphorylation, demonstrating that KIF17 motor activity drives in vivo NR2B transport and influences downstream transcriptional programs linked to learning and memory. Transgenic mouse overexpression (GFP-KIF17), behavioral assays, Western blot, CREB phosphorylation analysis PNAS High 12391294
2007 CaMKII phosphorylates KIF17 at Ser1029 in its tail domain, disrupting the KIF17–Mint1 interaction and releasing transported NR2B-containing cargo from the motor. FRET (direct visualization of protein-protein interaction), in vitro kinase assay, site-directed mutagenesis, co-immunoprecipitation Nature Cell Biology High 18066053
2011 Genetic deletion of kif17 in mice impairs NR2B transport and reduces nr2b transcription; it also reduces NR2A levels via accelerated ubiquitin-proteasome degradation. Synaptic NMDAR-mediated currents, LTP, LTD, CREB responses, and hippocampus-dependent memory are all attenuated in kif17−/− mice. Kif17 knockout mouse, electrophysiology, behavioral testing, Western blot, ubiquitin-proteasome inhibitor rescue experiments Neuron High 21521616
2012 Phosphorylation of KIF17 at Ser1029 by CaMKII is required bidirectionally for normal NR2B transport in vivo: phospho-null (S1029A) mice cannot load GluN2B cargo onto KIF17, while phospho-mimetic (S1029D) mice cannot unload it; both result in reduced synaptic NMDA receptors, impaired synaptic plasticity, and spatial memory deficits. Transgenic mice expressing S1029A or S1029D KIF17 in kif17−/− background, electrophysiology, behavioral assays, immunostaining The Journal of Neuroscience High 22514311
2010 KIF17 contains a ciliary localization signal (CLS) in its tail domain that is necessary and sufficient for ciliary targeting. The CLS is recognized by importin-β2, and this interaction is inhibited by RanGTP. Cytoplasmic expression of GTP-locked Ran(G19V) abolishes ciliary entry of KIF17, establishing that a Ran-GTP gradient (analogous to nuclear import) regulates KIF17 ciliary entry. CLS deletion/fusion constructs, importin-β2 co-immunoprecipitation, dominant-negative RanG19V expression, live-cell imaging Nature Cell Biology High 20526328
2006 KIF17 is required for ciliary targeting of olfactory CNG channels in mammalian olfactory sensory neurons; dominant-negative KIF17 disrupts CNG channel localization to olfactory cilia. Dominant-negative KIF17 expression, immunofluorescence in olfactory sensory neurons Current Biology Medium 16782012
2006 In C. elegans, OSM-3 (KIF17 ortholog) and kinesin-II cooperate via a mechanical competition mechanism to move IFT particles along microtubule doublets; purified OSM-3 and kinesin-II alone can recapitulate cooperative movement in vitro without additional regulatory factors. Competitive in vitro MT gliding assays with purified motors, in vivo IFT transport assays in BBS and motor mutants, quantitative modeling The Journal of Cell Biology High 17000880
2006 OSM-3 (C. elegans KIF17 ortholog) motility is autoinhibited in solution via intramolecular folding at a central hinge; a G444E point mutation in the hinge or hinge deletion relieves autoinhibition, activating ATPase and processive movement. Cargo attachment (bead in optical trap) also activates wild-type OSM-3 motility. Single-molecule fluorescence assay, MT-stimulated ATPase assay, optical trap, site-directed mutagenesis (G444E), EM/conformational analysis The Journal of Cell Biology High 17000874
2016 Polarized dendritic sorting of KIF17 requires three steps: (1) cargo binding relieves KIF17 autoinhibition and initiates microtubule transport; (2) the actin cytoskeleton at the axon initial segment (AIS) acts as a filter preventing KIF17-coupled vesicles from progressing into the axon; (3) dynein-based activity redirects KIF17 cargo into dendrites. Live-cell imaging, inducible trafficking assays (rapamycin-based), actin depolymerization, dynein inhibition in cultured neurons Current Biology High 27265394
2008 KIF17 (zebrafish Kif17) is essential for photoreceptor outer segment (OS) development; Kif17 knockdown severely disrupts OS formation and visual pigment targeting. Kif17 co-immunoprecipitates with IFT proteins within photoreceptor OS, establishing it as part of the IFT machinery in vertebrate photoreceptors. Morpholino knockdown in zebrafish, co-immunoprecipitation with IFT proteins, immunofluorescence Developmental Biology High 18304522
2005 KIF17 transports the K+ channel Kv4.2 to dendrites; dominant-negative KIF17 (but not dominant-negatives of other kinesins) dramatically inhibits dendritic localization of endogenous and introduced Kv4.2. Kv4.2 and KIF17 co-immunoprecipitate from brain lysate and from co-transfected COS cells, indicating direct or indirect interaction through the extreme C-terminus of Kv4.2. Dominant-negative constructs, co-immunoprecipitation from brain lysate and COS cells, colocalization in cortical neurons The Journal of Biological Chemistry Medium 16257958
2006 KIF17 interacts with kainate receptor subunits GluR6 and KA2 and is required for localization of GluR5 to distal dendrites; coimmunoprecipitation from hippocampal neurons and dominant-negative KIF17 experiments established this interaction and functional requirement. Co-immunoprecipitation from hippocampal neurons, dominant-negative KIF17 expression, immunofluorescence Molecular and Cellular Neurosciences Medium 17174564
2005 KIF17b (testis-specific isoform) controls the subcellular localization of the CREM transcriptional coactivator ACT between nucleus and cytoplasm in a manner that is independent of its motor domain and microtubules but dependent on protein kinase A-mediated phosphorylation of KIF17b. Truncation/deletion constructs, microtubule depolymerization (nocodazole), PKA inhibitor/activator experiments, subcellular fractionation The Journal of Biological Chemistry Medium 16002395
2003 KIF17b (testis-specific isoform) co-immunoprecipitates with TB-RBP (Translin) in an RNA-protein complex that contains specific CREM-regulated mRNAs, and KIF17b serves as the motor component transporting these mRNPs in postmeiotic male germ cells; dissociation of KIF17b and TB-RBP from mRNA is temporally linked to the time of translation. Co-immunoprecipitation, in vivo RNA-protein crosslinking, in situ hybridization combined with immunohistochemistry at EM level PNAS Medium 14673085
2006 KIF17b physically interacts with the PIWI/Argonaute family protein MIWI in chromatoid bodies of round spermatids; KIF17b localizes to chromatoid bodies and may provide microtubule-dependent mobility to these structures and facilitate loading of haploid RNAs. Co-immunoprecipitation (KIF17b–MIWI), immunofluorescence localization, analysis of miwi-null mouse spermatids Journal of Cell Science Medium 16787948
2017 KIF17 ciliary entry requires two independent determinants: (1) binding to the IFT46–IFT56 dimer of the IFT-B complex through a C-terminal sequence just upstream of the NLS, and (2) the NLS itself, to which importin-α proteins bind. KIF17 reaches the ciliary tip independently of its motor domain and is dispensable for ciliogenesis and IFT-B trafficking in mammalian cells. Visible immunoprecipitation (VIP) assay, deletion mutant analysis, importin-α co-immunoprecipitation, live-cell imaging of ciliary tip accumulation Molecular Biology of the Cell High 28077622
2015 Ciliary delivery of D1-type dopamine receptors from the extra-ciliary plasma membrane requires IFT-B, KIF17, and Rab23; Rab23 depletion prevents receptor ciliary access, and fusion of Rab23 to a non-ciliary receptor drives nucleotide-dependent mis-localization to cilia. siRNA knockdown of IFT-B, KIF17, Rab23; constitutively active/dominant-negative Rab23 fusions; live-cell imaging of receptor trafficking eLife High 26182404
2015 Rab23 forms a complex with KIF17 and importin-β2, and Rab23 depletion disrupts ciliary localization of KIF17; wild-type and constitutively active Rab23(Q68L) enrich at the primary cilium, suggesting Rab23 acts as an effector regulating KIF17 ciliary transport. Co-immunoprecipitation, affinity-binding assay (Rab23–KIF17–importin-β2 complex), siRNA knockdown, immunofluorescence Journal of Cell Science Medium 26136363
2013 The KIF17 motor domain (K370) directly stabilizes microtubule plus-ends and inhibits depolymerization independently of EB1; the KIF17 autoinhibitory tail domain binds microtubules and tubulin dimers and delays initial MT polymerization. EB1 and the KIF17 tail competitively interact with the motor domain and have opposing effects on MT-stimulated ATPase activity. In vitro MT dynamic instability assay, MT-stimulated ATPase assay, MT dilution depolymerization assay, MT cosedimentation, nocodazole washout in cells The Journal of Biological Chemistry High 24072717
2016 KIF17 localizes at epithelial cell-cell adhesions where it activates RhoA signaling through its motor domain (independently of microtubule binding), promoting junctional actin accumulation via the RhoA–ROCK–LIMK–cofilin pathway and stabilizing the apical junctional complex. KIF17 depletion (siRNA), overexpression of full-length and truncation mutants, dominant-negative RhoA, ROCK inhibitor, kinase-dead LIMK1, activated cofilin(S3A), GFP-actin incorporation assay, 3D organotypic culture Journal of Cell Science High 26759174
2016 Septin 9 (SEPT9) directly associates with the C-terminal tail of KIF17 (preferentially in the extended cargo-binding conformation), competes with the PDZ1 domain of mLin-10/Mint1 for KIF17 tail binding, and thereby down-regulates NR2B transport into hippocampal neuron dendrites without affecting KIF17 microtubule-based motility. Direct binding assay (GST pulldown with purified proteins), co-immunoprecipitation, live-cell imaging of KIF17 motility, NR2B immunostaining in neurons, siRNA knockdown Molecular Biology of the Cell High 26823018
2013 TTC30B (mouse ortholog of C. elegans DYF-1) interacts directly with KIF17 as shown by co-immunoprecipitation and cell-free in vitro expression co-IP, and also associates with multiple IFT-B complex proteins, suggesting a physical link between TTC30B and KIF17 within the IFT machinery. Co-immunoprecipitation from mouse pituitary cells, in vitro cell-free expression followed by Co-IP, mass spectrometry Experimental Cell Research Medium 23810713
2018 CaMKII-dependent phosphorylation of KIF17 at Ser815 (zebrafish equivalent of mouse Ser1029) promotes KIF17 localization along cone outer segments and drives outer segment disc shedding in a cell-autonomous manner; kif17 genetic mutants in zebrafish and mice exhibit diminished disc shedding, and constitutively active CaMKII increases disc shedding in a kif17-dependent manner. Transgenic zebrafish expressing phospho-mimetic/phospho-null KIF17, kif17 genetic mutants in zebrafish and mice, constitutively active CaMKII transgene, quantitative disc shedding assay BMC Cell Biology High 30458707
2019 GTP-bound Rab23 promotes migration of Kif17 to spindle poles during mouse oocyte meiosis. Depletion of Rab23 or Kif17 causes polar body extrusion failure, perturbs spindle formation, and disrupts tubulin acetylation via altered αTAT and Sirt2 expression. The Kif17 tail domain associates with constituents of the RhoA–ROCK–LIMK–cofilin pathway to regulate cytoplasmic actin and spindle migration. siRNA depletion of Rab23 and Kif17 in mouse oocytes, immunofluorescence for spindle markers, Western blot for αTAT/Sirt2, Co-immunoprecipitation of Kif17 tail with RhoA pathway components Development Medium 30696709
2020 KIF17 is locally degraded and synthesized in dendrites in an NMDAR-activity-dependent manner; activity-dependent local synthesis is driven by the KIF17 3'UTR at dendritic shafts, and newly synthesized KIF17 engages in dendritic transport. Hippocampus-specific deletion of Kif17 3'UTR impairs fear memory extinction by disrupting activity-dependent KIF17 synthesis. Live imaging of GFP-KIF17 synthesis in dendrites, 3'UTR reporter assays, NMDAR antagonist/agonist treatments, hippocampus-specific 3'UTR deletion mouse, fear conditioning and extinction behavioral assay Science Advances High 33328231
2021 Crystal structures of the OSM-3 (C. elegans KIF17 ortholog) motor domain in ADP-bound, nucleotide-free, and ATP-analog-bound states show that the nucleotide-binding site is virtually identical to that of Eg5 in the ATP-like state, demonstrating that a two-water-molecule ATP hydrolysis mechanism is conserved in kinesin-2. X-ray crystallography (structures 7A3Z, 7A40, 7A5E), structural comparison with Eg5 FEBS Open Bio High 33513284
2017 KIF17 interacts with estrogen-related receptor alpha (ERRα) via its C-terminal tail (containing an LXXLL nuclear receptor box) and the ERRα ligand-binding/AF2 domain; KIF17 tail expression attenuates nuclear accumulation of newly synthesized ERRα and inhibits ERRα transcriptional activity, while KIF17 knockout elevates ERRα activity. Yeast-2-hybrid screen, in vitro binding assay, KIF17 knockout cells, nuclear fractionation, reporter gene (transcriptional activity) assay, deletion/peptide constructs Oncotarget Medium 28881568
2024 KIF17 has dual and opposing roles in Hedgehog (HH)-dependent cerebellar development: in Purkinje cells (HH-producing), KIF17 promotes SHH protein levels and its cell-type-specific deletion reduces SHH and CGNP proliferation; in CGNPs (HH-responding), KIF17 deletion increases CGNP proliferation and HH target gene expression by altering GLI transcription factor processing. Germline Kif17 knockout mice, Purkinje cell-specific and CGNP-specific conditional Kif17 deletion, cerebellar morphometry, HH pathway target gene expression (qPCR/Western blot), GLI processing analysis Science Advances High 38669326
2025 In C. elegans, NEKL-3 (a NIMA-related kinase) directly phosphorylates the motor domain of OSM-3 (KIF17 ortholog), inhibiting its in vitro ATPase activity; NEKL-3 and NEKL-4 localize at the ciliary base and restrict OSM-3 activation to the distal ciliary region. Elevated protein phosphatase 2A at the transition zone triggers premature OSM-3 activation, while PP2A deficiency reduces OSM-3 activity and cilia length. In vitro kinase assay (NEKL-3 phosphorylation of OSM-3), fluorescence lifetime imaging microscopy (FLIM) for OSM-3 conformational state in vivo, genetic analysis of NEKL-3/4 and PP2A mutants, FRAP The Journal of Cell Biology High 40272473
1993 osm-3 encodes a kinesin heavy chain-like protein with conserved ATPase and microtubule-binding domains; osm-3 mutants accumulate dense matrix material in amphid sheath cytoplasm and have shortened distal cilia segments, establishing a kinesin-like role in axonal/ciliary transport in C. elegans chemosensory neurons. Germline transformation rescue, DNA sequencing, Northern blot, electron microscopy of cilia ultrastructure Neuroreport Medium 7690265
2022 KIF17 is modified by SUMOylation, which stabilizes and maintains KIF17 protein levels in epileptic neurons; KIF17 overexpression increases severity of KA-induced epileptic activity and elevates membrane NR2B expression, while KIF17 knockdown has the opposite effect. KIF17 overexpression/knockdown in mouse epilepsy model, SUMO modification assay, electrophysiology, NR2B membrane fractionation Neuroscience Bulletin Medium 35678994
2010 Nuclear respiratory factor 1 (NRF-1) directly binds conserved sites in the Kif17 gene promoter and functionally regulates Kif17 transcription; NRF-1 silencing blocks KCl-depolarization-induced Kif17 upregulation, while NRF-1 overexpression rescues TTX-suppressed Kif17 expression, co-regulating KIF17 with its cargo NR2B. EMSA, supershift assay, chromatin immunoprecipitation (ChIP), promoter mutation assay, NRF-1 siRNA knockdown and overexpression, real-time qPCR Biochimica et Biophysica Acta High 21171239

Source papers

Stage 0 corpus · 61 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 Kinesin superfamily motor protein KIF17 and mLin-10 in NMDA receptor-containing vesicle transport. Science (New York, N.Y.) 584 10846156
2010 Ciliary entry of the kinesin-2 motor KIF17 is regulated by importin-beta2 and RanGTP. Nature cell biology 224 20526328
2006 Ciliary targeting of olfactory CNG channels requires the CNGB1b subunit and the kinesin-2 motor protein, KIF17. Current biology : CB 175 16782012
2003 KIF17 dynamics and regulation of NR2B trafficking in hippocampal neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience 168 12514209
2006 Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors. The Journal of cell biology 158 17000880
2007 Disruption of KIF17-Mint1 interaction by CaMKII-dependent phosphorylation: a molecular model of kinesin-cargo release. Nature cell biology 154 18066053
2002 Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice. Proceedings of the National Academy of Sciences of the United States of America 147 12391294
2008 The homodimeric kinesin, Kif17, is essential for vertebrate photoreceptor sensory outer segment development. Developmental biology 119 18304522
2011 Molecular motor KIF17 is fundamental for memory and learning via differential support of synaptic NR2A/2B levels. Neuron 117 21521616
1995 Exclusive expression of C. elegans osm-3 kinesin gene in chemosensory neurons open to the external environment. Journal of molecular biology 103 7714894
2006 Interplay of PIWI/Argonaute protein MIWI and kinesin KIF17b in chromatoid bodies of male germ cells. Journal of cell science 102 16787948
1993 C. elegans osm-3 gene mediating osmotic avoidance behaviour encodes a kinesin-like protein. Neuroreport 102 7690265
2006 Autoinhibition regulates the motility of the C. elegans intraflagellar transport motor OSM-3. The Journal of cell biology 91 17000874
2005 A role for Kif17 in transport of Kv4.2. The Journal of biological chemistry 85 16257958
2015 Dopamine receptors reveal an essential role of IFT-B, KIF17, and Rab23 in delivering specific receptors to primary cilia. eLife 71 26182404
2003 The kinesin KIF17b and RNA-binding protein TB-RBP transport specific cAMP-responsive element modulator-regulated mRNAs in male germ cells. Proceedings of the National Academy of Sciences of the United States of America 70 14673085
2017 Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal. Molecular biology of the cell 51 28077622
2012 Regulation of NMDA receptor transport: a KIF17-cargo binding/releasing underlies synaptic plasticity and memory in vivo. The Journal of neuroscience : the official journal of the Society for Neuroscience 50 22514311
2009 Different roles for KIF17 and kinesin II in photoreceptor development and maintenance. Developmental dynamics : an official publication of the American Association of Anatomists 49 19384852
2006 Localization of glutamate receptors to distal dendrites depends on subunit composition and the kinesin motor protein KIF17. Molecular and cellular neurosciences 46 17174564
2015 A role for Rab23 in the trafficking of Kif17 to the primary cilium. Journal of cell science 38 26136363
2006 Dynamic distribution of Spatial during mouse spermatogenesis and its interaction with the kinesin KIF17b. Experimental cell research 36 17196196
2005 Microtubule-independent and protein kinase A-mediated function of kinesin KIF17b controls the intracellular transport of activator of CREM in testis (ACT). The Journal of biological chemistry 36 16002395
2012 The kinesin superfamily protein KIF17: one protein with many functions. Biomolecular concepts 35 23762210
2019 Rab23/Kif17 regulate meiotic progression in oocytes by modulating tubulin acetylation and actin dynamics. Development (Cambridge, England) 34 30696709
2016 Septin 9 interacts with kinesin KIF17 and interferes with the mechanism of NMDA receptor cargo binding and transport. Molecular biology of the cell 30 26823018
2018 Kif17 phosphorylation regulates photoreceptor outer segment turnover. BMC cell biology 27 30458707
2014 The inhibitor of calcium/calmodulin-dependent protein kinase II KN93 attenuates bone cancer pain via inhibition of KIF17/NR2B trafficking in mice. Pharmacology, biochemistry, and behavior 27 24836181
2022 Modulation of KIF17/NR2B crosstalk by tozasertib attenuates inflammatory pain in rats. Inflammopharmacology 25 35243557
2016 Three-Step Model for Polarized Sorting of KIF17 into Dendrites. Current biology : CB 25 27265394
2012 Prenatal stress disturbs hippocampal KIF17 and NR2B in spatial cognition in male offspring. Journal of neuroscience research 23 23239421
2022 Inhibition of pan-Aurora kinase attenuates evoked and ongoing pain in nerve injured rats via regulating KIF17-NR2B mediated signaling. International immunopharmacology 22 35183034
2017 Cos2/Kif7 and Osm-3/Kif17 regulate onset of outer segment development in zebrafish photoreceptors through distinct mechanisms. Developmental biology 19 28341548
2010 The kinesin superfamily protein KIF17 is regulated by the same transcription factor (NRF-1) as its cargo NR2B in neurons. Biochimica et biophysica acta 19 21172391
2016 A Screen for Modifiers of Cilia Phenotypes Reveals Novel MKS Alleles and Uncovers a Specific Genetic Interaction between osm-3 and nphp-4. PLoS genetics 17 26863025
2013 Direct regulation of microtubule dynamics by KIF17 motor and tail domains. The Journal of biological chemistry 16 24072717
2012 Analysis of KIF17 distal tip trafficking in zebrafish cone photoreceptors. Vision research 16 23099049
2009 The presence of kinesin superfamily motor proteins KIFC1 and KIF17 in normal and pathological human placenta. Placenta 16 19679349
2008 Altered expression of KIF17, a kinesin motor protein associated with NR2B trafficking, may mediate learning deficits in a Down syndrome mouse model. American journal of obstetrics and gynecology 15 18313453
2016 KIF17 regulates RhoA-dependent actin remodeling at epithelial cell-cell adhesions. Journal of cell science 14 26759174
2014 Intrathecal injection of selected peptide Myr-RC-13 attenuates bone cancer pain by inhibiting KIF17 and NR2B expression. Pharmacology, biochemistry, and behavior 13 24726705
2020 An activity-dependent local transport regulation via degradation and synthesis of KIF17 underlying cognitive flexibility. Science advances 12 33328231
2013 Interaction of mouse TTC30/DYF-1 with multiple intraflagellar transport complex B proteins and KIF17. Experimental cell research 12 23810713
2022 KIF17 Modulates Epileptic Seizures and Membrane Expression of the NMDA Receptor Subunit NR2B. Neuroscience bulletin 11 35678994
2018 KIF17 mediates the learning and memory impairment in offspring induced by maternal exposure to propofol during middle pregnancy. Molecular medicine reports 9 29393422
2007 Neuronal distribution of spatial in the developing cerebellum and hippocampus and its somatodendritic association with the kinesin motor KIF17. Experimental cell research 9 17961552
2022 Betaine Attenuates Chronic Constriction Injury-Induced Neuropathic Pain in Rats by Inhibiting KIF17-Mediated Nociception. ACS chemical neuroscience 7 36367842
2015 A decrease in protein level and a missense polymorphism of KIF17 are associated with schizophrenia. Psychiatry research 7 26421900
2014 The cyclic AMP response element-binding protein antisense oligonucleotide induced anti-nociception and decreased the expression of KIF17 in spinal cord after peripheral nerve injury in mice. International journal of clinical and experimental medicine 7 25664020
2023 Sida cordifolia L. attenuates behavioral hypersensitivity by interfering with KIF17-NR2B signaling in rat model of neuropathic pain. Journal of ethnopharmacology 6 37640257
2021 R869C mutation in molecular motor KIF17 gene is involved in dementia with Lewy bodies. Alzheimer's & dementia (Amsterdam, Netherlands) 6 34124335
2017 Modulation of estrogen related receptor alpha activity by the kinesin KIF17. Oncotarget 6 28881568
2000 C. elegans KLP-11/OSM-3/KAP-1: orthologs of the sea urchin kinesin-II, and mouse KIF3A/KIFB/KAP3 kinesin complexes. DNA research : an international journal for rapid publication of reports on genes and genomes 6 10819327
2025 Phosphorylation-dependent regional motility of the ciliary kinesin OSM-3. The Journal of cell biology 5 40272473
2021 Structural snapshots of the kinesin-2 OSM-3 along its nucleotide cycle: implications for the ATP hydrolysis mechanism. FEBS open bio 5 33513284
2021 Biallelic Variants in KIF17 Associated with Microphthalmia and Coloboma Spectrum. International journal of molecular sciences 5 33922911
2022 The potential function of KIF17 in large yellow croaker (Larimichthys crocea) spermatid remodeling: molecular characterization and expression pattern during spermiogenesis. Fish physiology and biochemistry 4 35538183
2024 Dual and opposing roles for the kinesin-2 motor, KIF17, in Hedgehog-dependent cerebellar development. Science advances 3 38669326
2023 Expression Dynamics Indicate Potential Roles of KIF17 for Nuclear Reshaping and Tail Formation during Spermiogenesis in Phascolosoma esculenta. International journal of molecular sciences 3 38203305
2022 KIF17 maintains the epithelial phenotype of breast cancer cells and curbs tumour metastasis. Cancer letters 2 36089118
2025 GRIN2B alleviates mid-gestational sevoflurane exposure-induced early differentiation of rat neural stem cells by interacting with KIF17. Journal of cell communication and signaling 1 40568341

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