Showing SEPTIN9 — SEPT9 is a alias.
- Length
- 586 aa
- Mass
- 65.4 kDa
- Annotated
- 2026-06-13
41 papers in source corpus
12 papers cited in narrative
12 extracted findings
Mechanistic narrative
Synthesis pass · prose summary of the discoveries below
Parse failed — see logs
Mechanism profile
Synthesis pass · controlled-vocabulary classification · explore literature graph →
No controlled-vocabulary terms were assigned to this entry.
Evidence
Reading pass · 12 per-paper findings extracted from the source corpus
| Year | Finding | Method | Journal | Conf | PMIDs |
|---|---|---|---|---|---|
| 1999 | AF17q25 (SEPTIN9) is a putative septin family GTPase that fuses with MLL in acute myeloid leukemia bearing t(11;17)(q23;q25), producing an MLL-AF17q25 fusion transcript. The AF17q25 protein is homologous to septin family members including H5, NEDD5, CDC10, and hCDCrel. | Molecular cloning, RT-PCR, sequence analysis of fusion transcripts from AML patient samples | Cancer research | Medium | 10485469 |
| 2011 | SEPTIN9 is required for septin filament formation and cellular stability: loss of Sept9 in murine embryonic fibroblasts abolishes long SEPT7-containing filaments (replaced by short/curved filaments and rings), mislocalizes SEPT7 from the mitotic spindle, impairs cell migration and hypo-osmotic stress resistance, and reduces stress fibers and focal adhesion-associated vinculin staining. | Conditional knockout in immortalized MEFs, immunofluorescence for SEPT7/vinculin/stress fibers, gap-filling and filter-invasion migration assays, osmotic stress assays | Biological chemistry | High | 21824004 |
| 2013 | SEPTIN9 is required for normal T-cell development and CD8+ T-cell homeostasis: T-cell-specific conditional deletion of Sept9 results in increased double-negative thymocytes, decreased peripheral mature T-cells, reduced CD8+ T-cell proliferation upon stimulation, and expansion of CD8+ central memory T-cells. | Conditional knockout (Cre/loxP), flow cytometry (FACS), in vitro proliferation assay | Cell and tissue research | High | 23644740 |
| 2013 | SEPTIN9 protein is localized specifically to the cytoplasm of neurons (not astrocytes or oligodendrocytes) in rat brain, and co-localizes with active caspase-3 in the peritrauma cortex following traumatic brain injury, with expression levels paralleling active caspase-3 upregulation. | Western blot, immunohistochemistry, immunofluorescence co-localization, quantitative RT-PCR in rat TBI model | Journal of molecular neuroscience : MN | Low | 23700216 |
| 2016 | DNMT3a-mediated hypermethylation of the Sept9 promoter suppresses SEPTIN9 protein expression in hepatic stellate cells (HSCs) during liver fibrogenesis. SEPTIN9 loss promotes fibrosis (increases α-SMA, Col1a1, and extracellular matrix accumulation), while SEPTIN9 overexpression attenuates TGF-β1-induced myofibroblast activation and promotes HSC apoptosis. | Reduced representation bisulfite sequencing (RRBS), bisulfite pyrosequencing, DNMT3a-siRNA knockdown, 5-azadC treatment, SEPTIN9 overexpression and RNAi in HSC-T6 cells, CCl4-treated mouse model, western blotting | Toxicology and applied pharmacology | High | 27939986 |
| 2018 | Knockdown of SEPTIN9 (and SEPT2) in GBM cell lines inhibits cell proliferation, arrests cell cycle in S phase, decreases invasive capability, and impairs xenograft tumor growth; these effects involve the p53/p21 axis and MEK/ERK signaling pathways. | RNAi knockdown in A172/U87-MG GBM cell lines, proliferation assay, cell cycle analysis, invasion assay, nude mouse xenograft, western blotting for p53/p21 and MEK/ERK | Cell death & disease | Medium | 29724999 |
| 2020 | SEPTIN9 acts as a negative regulator of ARHGAP4 (a Rho-GAP) and promotes epithelial-to-mesenchymal transition (EMT) by activating the FAK/Src signaling pathway. SEPTIN9 was identified as an ARHGAP4-interacting protein by proteomic analysis and its role in suppressing ARHGAP4 activity was functionally validated. | Comprehensive Rho-GAP siRNA screen, proteomic analysis (mass spectrometry) of ARHGAP4 interactors identifying SEPTIN9, multidisciplinary functional assays (cell morphology, EMT marker expression, proliferation, migration, 3D morphogenesis, focal adhesion/stress fiber analysis, FAK/Src pathway western blot) | FASEB journal | Medium | 32378260 |
| 2022 | SEPTIN9 is dispensable for septin multimer formation in peripheral nervous system (PNS) myelin Schwann cells: Schwann cell-specific deletion of Septin9 did not affect the abundance or localization of other septin subunits (SEPTIN2, SEPTIN7, SEPTIN8, SEPTIN11). In contrast, Schwann cell-specific deletion of Septin2 markedly reduced all relevant septin subunits including SEPTIN9, indicating SEPTIN2 (not SEPTIN9) is required for septin multimer formation/stabilization in PNS myelin. | Cre/loxP conditional knockout of Septin9 and Septin2 specifically in Schwann cells, immunofluorescence and western blot for septin subunit abundance and localization in sciatic nerves, nerve conduction velocity measurement, motor/sensory behavioral testing | Cytoskeleton (Hoboken, N.J.) | High | 36378242 |
| 2025 | SEPTIN9 localizes to mitochondrial fission sites from early stages (co-occurring with the ER and prior to DRP1 recruitment) and plays an isoform-specific role in mitochondrial fission. SEPTIN9 N-terminal domain activates the Rho guanine nucleotide exchange factor ARHGEF18; loss of SEPTIN9 impairs mitochondrial calcium influx, indicating that SEPTIN9-containing octamers are required for early stages of mitochondrial fission. | Live-cell imaging of SEPTIN9 at mitochondrial fission sites, loss-of-function experiments, isoform-specific rescue experiments, functional assays for mitochondrial calcium influx (calcium imaging), identification of N-terminal domain interaction with ARHGEF18 | The Journal of cell biology | High | 40920138 |
| 2025 | SEPTIN9 directly interacts with Ebola virus VP35 protein via the C-terminal region of VP35. SEPTIN9 knockdown or depletion significantly suppresses EBOV replication and disrupts formation of viral inclusion bodies (VIBs), as shown by EBOV minigenome and trVLP systems. | VP35-TurboID proximity labeling coupled with quantitative mass spectrometry, western blot and Far-Western blot validation of direct interaction, EBOV minigenome system, transcription- and replication-competent VLP (trVLP) system, SEPTIN9 knockdown | Sheng wu gong cheng xue bao = Chinese journal of biotechnology | Medium | 40873324 |
| 2026 | SEPTIN9 localizes to cortical microtubules (rather than actin filaments) in epithelial cells and drives microtubule-templated actin assembly required for apical domain expansion and ciliogenesis. SEPTIN9 and microtubules form a mutually stabilizing scaffold; the microtubule-binding function of SEPTIN9 is genetically separable from its actin-binding function, and microtubule association is specifically required for actin assembly. | Xenopus multiciliated cell model, SEPTIN9 depletion, rescue with human SEPT9 isoforms, live-cell imaging, pharmacological microtubule/actin disruption, isoform-specific depletion and rescue experiments separating microtubule vs actin binding functions | bioRxivpreprint | Medium | 42239308 |
| 2026 | SEPTIN9 suppression in bladder cancer cell lines reduces cell proliferation, invasion, and migration, and shifts EMT markers (decreases N-cadherin and vimentin, increases E-cadherin), indicating a role for SEPTIN9 in promoting EMT in bladder cancer. | siRNA knockdown, colony formation assay, transwell invasion assay, wound-healing assay, qRT-PCR and western blot for EMT markers | Urologic oncology | Low | 41951453 |
Source papers
Stage 0 corpus · 41 papers · ranked by NIH iCite citations
| Year | Title | Journal | Citations | PMID |
|---|---|---|---|---|
| 2014 | Plasma Septin9 versus fecal immunochemical testing for colorectal cancer screening: a prospective multicenter study. | PloS one | 128 | 24901436 |
| 1999 | AF17q25, a putative septin family gene, fuses the MLL gene in acute myeloid leukemia with t(11;17)(q23;q25). | Cancer research | 77 | 10485469 |
| 2011 | Septin9 is involved in septin filament formation and cellular stability. | Biological chemistry | 63 | 21824004 |
| 2016 | Promoter hypermethylation of HS3ST2, SEPTIN9 and SLIT2 combined with FGFR3 mutations as a sensitive/specific urinary assay for diagnosis and surveillance in patients with low or high-risk non-muscle-invasive bladder cancer. | BMC cancer | 60 | 27586786 |
| 2018 | Repression of Septin9 and Septin2 suppresses tumor growth of human glioblastoma cells. | Cell death & disease | 41 | 29724999 |
| 2016 | Methylation of Septin9 mediated by DNMT3a enhances hepatic stellate cells activation and liver fibrogenesis. | Toxicology and applied pharmacology | 40 | 27939986 |
| 2022 | Methylated Septin9 (mSEPT9): A promising blood-based biomarker for the detection and screening of early-onset colorectal cancer. | Cancer research communications | 34 | 35992328 |
| 2018 | Diagnostic Assessment of septin9 DNA Methylation for Colorectal Cancer Using Blood Detection: A Meta-Analysis. | Pathology oncology research : POR | 26 | 30488278 |
| 2016 | The Relationship between the Methylated Septin-9 DNA Blood Test and Stool Occult Blood Test for Diagnosing Colorectal Cancer in Taiwanese People. | Journal of clinical laboratory analysis | 26 | 27390118 |
| 2020 | Comprehensive analysis on the whole Rho-GAP family reveals that ARHGAP4 suppresses EMT in epithelial cells under negative regulation by Septin9. | FASEB journal : official publication of the Federation of American Societies for Experimental Biology | 24 | 32378260 |
| 2016 | Diagnostic Value of Methylated Septin9 for Colorectal Cancer Screening: A Meta-Analysis. | Medical science monitor : international medical journal of experimental and clinical research | 22 | 27665580 |
| 2017 | A pooled analysis of the diagnostic efficacy of plasmic methylated septin-9 as a novel biomarker for colorectal cancer. | Biomedical reports | 21 | 29085631 |
| 2024 | Diagnostic performances of methylated septin9 gene, CEA, CA19-9 and platelet-to-lymphocyte ratio in colorectal cancer. | BMC cancer | 17 | 39068425 |
| 2013 | Septin9 is involved in T-cell development and CD8+ T-cell homeostasis. | Cell and tissue research | 16 | 23644740 |
| 2011 | Distinct functions of evolutionary conserved MSF1 and late embryogenesis abundant (LEA)-like domains in mitochondria. | The Journal of biological chemistry | 14 | 21930701 |
| 2022 | Performance of circulating methylated Septin9 gene DNA in diagnosis and recurrence monitoring of colorectal cancer in Western China. | Clinica chimica acta; international journal of clinical chemistry | 13 | 36330944 |
| 2021 | Dual-modality loop-mediated isothermal amplification for pretreatment-free detection of Septin9 methylated DNA in colorectal cancer. | Mikrochimica acta | 13 | 34453211 |
| 2023 | The stool syndecan2 methylation test is more robust than blood tests for methylated septin9, CEA, CA19-9 and CA724: a diagnostic test for the early detection of colorectal neoplasms. | Translational cancer research | 11 | 36760372 |
| 2022 | Septin9 DNA methylation as a promising biomarker for cervical cancer. | Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology | 11 | 36476308 |
| 2019 | Methylated SEPTIN9 plasma test for colorectal cancer detection may be applicable to Lynch syndrome. | BMJ open gastroenterology | 11 | 31275589 |
| 2018 | Study on early diagnosis of epithelial ovarian cancer by analysis of plasma septin-9 and clusterin level. | Journal of cancer research and therapeutics | 11 | 29970704 |
| 2014 | [Significance of Septin9 gene methylation detection of plasma circulation DNA in colorectal cancer screening]. | Zhonghua yi xue za zhi | 9 | 25623318 |
| 2024 | Septin9 DNA methylation is associated with breast cancer recurrence or metastasis. | The Journal of international medical research | 7 | 38180895 |
| 2023 | Methylation of Septin9, SRSF1, and PAX8 in Early Screening of Colorectal Cancer in the Population Undergoing Physical Examinations. | Clinical laboratory | 7 | 38084698 |
| 2023 | External quality assessment for detection of colorectal cancer by Septin9 DNA methylation in clinical laboratories. | Clinica chimica acta; international journal of clinical chemistry | 6 | 38008152 |
| 2013 | The expression patterns of Septin-9 after traumatic brain injury in rat brain. | Journal of molecular neuroscience : MN | 6 | 23700216 |
| 2024 | A novel dual-target Septin9 methylation assay for improved detection of early-stage colorectal cancer and high-grade intraepithelial neoplasia. | BMC cancer | 4 | 39080571 |
| 2022 | SEPTIN9-SDC2-VIM methylation signature as a biomarker for the early diagnosis of colorectal cancer. | American journal of cancer research | 4 | 35968354 |
| 2022 | Targeted inactivation of the Septin2 and Septin9 genes in myelinating Schwann cells of mice. | Cytoskeleton (Hoboken, N.J.) | 4 | 36378242 |
| 2025 | SEPTIN9 locally activates the RhoGEF ARHGEF18 to promote early stages of mitochondrial fission. | The Journal of cell biology | 3 | 40920138 |
| 2015 | [Protein levels and its clinical significance of septin-9 and clusterin in peripheral blood of epithelial ovarian cancer patients]. | Zhonghua fu chan ke za zhi | 3 | 26675395 |
| 2023 | Analyzing the Role of Septin9 Gene Methylation in the Diagnosis and Treatment of Primary Liver Cancer in the Elderly. | Alternative therapies in health and medicine | 2 | 36947654 |
| 2026 | Synergistic CRISPR/Cas12a-Nanozyme System for Iontronic Sensing of Site-Specific Septin9 Methylation. | Analytical chemistry | 1 | 41548240 |
| 2026 | Development and validation of machine learning-based models integrating Septin9 methylation and serum biomarkers for early detection and differentiation of colorectal cancer. | PeerJ | 0 | 41940389 |
| 2026 | Repression of Septin9 inhibits the oncogenic phenotype of bladder cancer. | Urologic oncology | 0 | 41951453 |
| 2026 | Microtubule-templated actin assembly by septin9 drives apical expansion of epithelial cells. | bioRxiv : the preprint server for biology | 0 | 42239308 |
| 2025 | Pre-Treatment SEPTIN9 Gene Methylation Ratio Predicts Tumor Response to Total Neoadjuvant Therapy in Patients with Locally Advanced Rectal Cancer. | Cancers | 0 | 40149300 |
| 2025 | Detecting plasma SHOX2, HOXA9, SEPTIN9, and RASSF1A methylation and circulating cancer cells for cholangiocarcinoma clinical diagnosis and monitoring. | World journal of gastrointestinal oncology | 0 | 40235897 |
| 2025 | Case Report: Parsonage-Turner syndrome due to SEPTIN9 mutation: report of an Italian family with childhood onset and review of the literature. | Frontiers in pediatrics | 0 | 40852410 |
| 2025 | [Septin9 promotes viral replication by interacting with Ebola virus VP35 to regulate inclusion body formation]. | Sheng wu gong cheng xue bao = Chinese journal of biotechnology | 0 | 40873324 |
| 2025 | Diagnostic efficacy and subgroup heterogeneity of SHOX2, RASSF1A, Septin9, and HOXA9 methylation in pleural effusion lymphoma. | Clinical epigenetics | 0 | 41225534 |
Missed literature
Know a paper Affinage missed for SEPTIN9? Flag it for the maintainers and the community.
No submissions yet.