| 2001 |
IL-24 (MDA-7/MOB-5) binds to and signals through two heterodimeric receptor complexes: IL-22R1/IL-20R2 and IL-20R1/IL-20R2 (the latter being the same receptor used by IL-20). COS cells transfected with either receptor heterodimer bind IL-24 with similar saturation kinetics, and IL-24 binding to either receptor complex on keratinocytes or ectopically expressing BHK cells activates STAT transcription factors. |
Ligand binding assay (COS cell transfection, saturation kinetics), STAT activation assay in keratinocytes and BHK cells |
The Journal of biological chemistry |
High |
11706020
|
| 2011 |
The IL-20–IL-20R1–IL-20R2 ternary complex was successfully purified and crystallized, forming a 1:1:1 complex that diffracted to 3 Å resolution, establishing the stoichiometry and feasibility of structural determination of the IL-20/IL-20R1/IL-20R2 signaling complex. |
Protein purification, crystallization, preliminary X-ray diffraction analysis |
Acta crystallographica. Section F, Structural biology and crystallization communications |
Medium |
22232181
|
| 2014 |
IL-20 signals through IL-20R1-containing receptor complexes to activate hepatic stellate cells, upregulate TGF-β1, TNF-α, and type I collagen expression, promote HSC proliferation and migration, and induce hepatocyte cell-cycle arrest. IL-20R1-deficient mice were protected from both short-term and long-term CCl4-induced liver injury, establishing IL-20R1 as required for IL-20-mediated hepatic fibrosis. |
Anti-IL-20R1 monoclonal antibody (51D) treatment in vivo; IL-20R1-deficient mouse model; in vitro HSC activation assays; CCl4 liver injury model |
Hepatology (Baltimore, Md.) |
High |
24763901
|
| 2019 |
IL-24 binding to receptor 1 subunits (IL-20R1 and IL-22R1) is governed by a flexible region around residue T198 in IL-24; a single back-engineered wild-type residue (T198) restored 80% of binding affinity to IL-20R1 and IL-22R1 and restored signaling capacity, while affinity to IL-20R2 was preserved in stabilized IL-24 variants. |
Protein engineering (PROSS algorithm-based mutagenesis), biophysical binding assays to extracellular receptor domains, signaling capacity assay, crystal structure of IL-24 variant (PDB 6GG1) |
The FEBS journal |
High |
31152679
|
| 2019 |
In intestinal lymphatic endothelial cells from Crohn's disease patients, mTOR signaling drives upregulation of IL-20RA, and IL-20RA-mediated intracellular signaling is required for LPMC transmigration through the lymphatic endothelial barrier; blocking this pathway reduced leukocyte trafficking. |
Transcriptomic profiling of isolated human intestinal lymphatic endothelial cells, transwell co-culture transmigration assay, mTOR pathway manipulation |
Cells |
Medium |
31426584
|
| 2021 |
IL-20RA activates the JAK1–STAT3–SOX2 signaling axis in breast cancer cells, enhancing stemness (increased SP proportion, ALDH activity, sphere formation, Sox2/Oct4 expression) and promoting PD-L1 expression while reducing CD8+ T cell and NK cell recruitment and increasing MDSC proportions in the tumor microenvironment. |
Gain- and loss-of-function (overexpression and knockdown) in breast cancer cell lines and mouse models; ELISA; flow cytometry; in vivo tumor initiation and metastasis assays |
Theranostics |
Medium |
33456560
|
| 2021 |
MIR452 directly targets IL-20RA (confirmed by luciferase reporter assay); overexpression of MIR452 decreases IL-20RA protein and downstream JAK1 and STAT3 (but not STAT1), and IL-20RA knockdown similarly decreases JAK1 and STAT3, placing IL-20RA upstream of JAK1–STAT3 (but not JAK1–STAT1) signaling in colorectal cancer cells. |
Luciferase reporter assay (miR-452 targeting IL-20RA 3'UTR), siRNA knockdown, Western blot, RT-PCR |
Inflammation research |
Medium |
34283251
|
| 2021 |
IL-20R1 mediates hematoma resolution after germinal matrix hemorrhage via the IL-20R1/ERK/Nrf2 pathway: rIL-19 treatment upregulated ERK, Nrf2, and CD163 expression, while IL-20R1 CRISPR knockdown abolished these effects, demonstrating that IL-20R1 is required for rIL-19-mediated scavenger receptor CD163 upregulation. |
In vivo rat GMH model; intranasal rIL-19 administration; IL-20R1 CRISPR knockdown (intracerebroventricular); Western blot; immunohistochemistry; hemoglobin assay; neurobehavioral testing |
Oxidative medicine and cellular longevity |
Medium |
33532035
|
| 2018 |
CRISPR/Cas9 deletion of a genomic region containing variant rs6927172 (located ~140 kb upstream of TNFAIP3) altered expression of IL-20RA; EMSA and chromatin conformation capture demonstrated that the DNA element carrying rs6927172 physically interacts with the IL-20RA locus and that the risk allele enhances NFκB binding and chromatin looping to regulate IL-20RA expression. |
CRISPR/Cas9 knockout in HEK293T cells, EMSA, Western blot, chromatin conformation capture (3C), TALE-based transcriptional analysis |
Genes and immunity |
Medium |
29483615
|
| 2022 |
In grass carp, IL-20R1 (CiIL-20R1/CRFB8) but not IL-20R2 is responsible for STAT3 phosphorylation downstream of IL-20 signaling; co-immunoprecipitation showed that IL-20 binds CiIL-20R2 but not CiIL-20R1, suggesting a division of labor where R2 captures ligand and R1 transduces the intracellular STAT3 signal. Structural modeling showed conservation of key residues with human IL-20R1. |
Co-immunoprecipitation, STAT3 phosphorylation assay, structural modeling |
Fish & shellfish immunology |
Medium |
36414129
|
| 2024 |
In fish (crucian carp hybrid), WR-IL-26 forms a complex with both WR-IL10R2 and WR-IL20R1 (co-immunoprecipitation); silencing WR-IL20R1 via RNA interference significantly attenuated IL-26-mediated JAK1–STAT3 pathway activation and reduced gut mucosal barrier protection against Aeromonas hydrophila infection. |
Co-immunoprecipitation, RNA interference knockdown, JAK1–STAT3 signaling assay, in vivo bacterial challenge |
Developmental and comparative immunology |
Medium |
39154973
|
| 2025 |
IL-20RA knockdown in nephroblastoma (Wilms tumor) cells increased apoptosis and ferroptosis, and IL-20RA promotes epithelial-mesenchymal transition through the STAT3/SNAIL pathway. |
siRNA knockdown, apoptosis/ferroptosis assays, EMT marker analysis, Western blot |
Scientific reports |
Low |
40185911
|
| 2024 |
In pig Sertoli cells, IL-20RA knockdown (siRNA) affected mitochondrial superoxide production and catalase secretion, identifying IL-20RA as a regulator of mitochondrial antioxidant capacity in these cells. |
siRNA knockdown, ROS measurement, mitochondrial superoxide assay, catalase activity assay, transcriptomic analysis |
Antioxidants (Basel, Switzerland) |
Low |
39765872
|