Affinage

HSPA1B

Heat shock 70 kDa protein 1B · UniProt P0DMV9

Length
641 aa
Mass
70.1 kDa
Annotated
2026-06-10
100 papers in source corpus 25 papers cited in narrative 25 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

HSPA1B (HSP70-2) is an ATP-dependent molecular chaperone with an essential, dedicated role in male meiosis and spermatogenesis: targeted disruption in mice arrests meiotic progression, blocks synaptonemal complex desynapsis, and triggers pachytene-stage spermatocyte apoptosis and male infertility, whereas female fertility is spared (PMID:8622925, PMID:9409676, PMID:8601336). Mechanistically, HSP70-2 chaperones CDC2 kinase, physically associating with it in testis, driving CDC2/cyclin B1 complex assembly and kinase activity; adding HSP70-2 to Hsp70-2-/- extracts restores both complex formation and activity, explaining the G2/M-transition failure (PMID:9247342). It localizes to both cytoplasm and nucleus of spermatocytes and associates with synaptonemal complexes specifically in males (PMID:8601336), and in post-meiotic spermatids acquires a second chaperone function, associating with transition proteins 1 and 2 to support genome condensation (PMID:17035236). Its chaperone cycle is supported by DnaJ/Hsp40 cochaperones MSJ-1 and DjA4, the latter stimulating its ATPase activity and suppressing protein aggregation (PMID:11466217, PMID:15047721). HSP70-2 abundance is set both post-translationally — BAT3/Scythe protects it from polyubiquitylation and proteasomal degradation (PMID:18678708) — and transcriptionally, through a spermatocyte-specific promoter (PMID:8631503, PMID:14766014) and stress-responsive cis-elements including a tonicity-responsive enhancer and a HIF-1alpha-bound hypoxia-responsive element (PMID:16819288, PMID:18844219); HSF1/HSF2 and RNA Pol II bookmark the promoter in spermatozoa for rapid post-fertilization expression (PMID:18434628, PMID:19336471, PMID:21053113). In cancer cells HSP70-2 promotes survival: its depletion triggers LEDGF-dependent lysosomal membrane permeabilization and cathepsin-mediated death, and mitochondria-dependent apoptosis, and supports tumor cell motility, invasion, and growth (PMID:17363574, PMID:19914824, PMID:18822209).

Mechanistic history

Synthesis pass · year-by-year structured walk · 9 steps
  1. 1996 High

    Established that HSP70-2 is not a redundant stress chaperone but is specifically essential for male meiosis, defining its core biological role.

    Evidence Targeted gene knockout in mice with synaptonemal complex immunocytology and nuclear fractionation

    PMID:8601336 PMID:8622925

    Open questions at the time
    • Molecular substrate of the meiotic arrest not yet defined at this stage
    • Sexual dimorphism of synaptonemal complex association unexplained
  2. 1997 High

    Resolved the cellular lesion as a failure of synaptonemal complex desynapsis, pinpointing the precise prophase I step requiring HSP70-2.

    Evidence Knockout mouse staged spermatogenesis with synaptonemal complex immunostaining

    PMID:9409676

    Open questions at the time
    • Did not identify the chaperone client driving desynapsis
  3. 1997 High

    Identified CDC2 kinase as a functional client, explaining the G2/M-transition block at the molecular level via in vitro reconstitution with KO extracts.

    Evidence Co-IP of endogenous complex, in vitro reconstitution with Hsp70-2-/- testis extracts, histone H1 kinase assay

    PMID:9247342

    Open questions at the time
    • Structural basis of HSP70-2/CDC2 recognition unresolved
    • Whether desynapsis defect is fully downstream of CDC2 inactivation not established
  4. 2004 Medium

    Characterized the cochaperone machinery supporting the HSP70-2 ATPase cycle and revealed functional non-equivalence among Hsp40 partners.

    Evidence In vitro ATPase, luciferase refolding and aggregation-suppression assays with purified DjA4 and MSJ-1 co-IP from spermatogenic cells

    PMID:11466217 PMID:15047721

    Open questions at the time
    • In vivo requirement of each cochaperone for fertility not tested
    • MSJ-1 partnership shown by single lab without genetic confirmation
  5. 2006 Medium

    Extended HSP70-2 function beyond meiosis by identifying transition proteins 1 and 2 as post-meiotic clients in genome condensation.

    Evidence Mass-spectrometry proteomics of condensing spermatids with co-association studies

    PMID:17035236

    Open questions at the time
    • Direct chaperone activity on transition proteins not reconstituted
    • Functional consequence of loss for condensation not genetically isolated
  6. 2008 High

    Defined post-translational control of HSP70-2 abundance, showing BAT3 protects it from proteasomal degradation independent of transcription.

    Evidence Bat3 knockout mice, ubiquitylation assay, proteasome inhibitor rescue

    PMID:18678708

    Open questions at the time
    • E3 ligase mediating polyubiquitylation not identified
  7. 2009 High

    Mapped transcriptional control of HSP70-2 to defined stress-responsive cis-elements and bookmarking factors, linking it to osmotic and hypoxic stress and post-fertilization expression.

    Evidence Promoter deletion/mutagenesis reporters, ChIP of HIF-1alpha, HSF1/HSF2 and Pol II, EMSA and transgenic promoter dissection

    PMID:14766014 PMID:16819288 PMID:18434628 PMID:18844219 PMID:19336471 PMID:8631503

    Open questions at the time
    • Interplay among TonE, HRE, and HSE elements not integrated
    • In vivo significance of spermatozoal bookmarking for embryo not directly tested
  8. 2010 Medium

    Demonstrated a pro-survival, oncogenic role in cancer cells through lysosomal stabilization and anti-apoptotic mechanisms.

    Evidence siRNA/shRNA knockdown in carcinoma lines with lysosomal permeabilization, cathepsin/cytochrome c readouts, LEDGF rescue, and xenografts

    PMID:17363574 PMID:18822209 PMID:19914824

    Open questions at the time
    • Direct molecular link between HSP70-2 and LEDGF not established
    • Single-lab cancer phenotypes
  9. 2022 Medium

    Added biophysical detail on a temperature-dependent UBQLN2 interaction and a 3'-UTR/miRNA layer of post-transcriptional control under stress.

    Evidence Tryptophan fluorescence and in vitro binding with WT/ALS-FTD UBQLN2; dual-luciferase miR-23a-5p 3'-UTR assay; reporter assay of 3'-UTR variant

    PMID:12393191 PMID:33206547 PMID:36423739

    Open questions at the time
    • Cellular consequence of UBQLN2 binding for HSPA1B function untested
    • miR-23a-5p axis characterized in renal context only

Open questions

Synthesis pass · forward-looking unresolved questions
  • How HSP70-2's well-defined meiotic chaperone role mechanistically connects to its cancer survival and stress-signaling (AKT, renal fibrosis) functions remains unresolved.
  • No unifying client/substrate map across tissues
  • Structural basis of client recognition unknown
  • Relationship between meiotic and somatic functions not integrated

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0044183 protein folding chaperone 3 GO:0140657 ATP-dependent activity 2 GO:0140096 catalytic activity, acting on a protein 1
Localization
GO:0005694 chromosome 2 GO:0005634 nucleus 1 GO:0005829 cytosol 1
Pathway
R-HSA-1474165 Reproduction 3 R-HSA-392499 Metabolism of proteins 2 R-HSA-5357801 Programmed Cell Death 2 R-HSA-8953897 Cellular responses to stimuli 2 R-HSA-1640170 Cell Cycle 1
Partners
BAG6CCNB1CDK1DNAJA4DNAJB3TNP1TNP2UBQLN2

Evidence

Reading pass · 25 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1996 HSP70-2 (HSPA1B) is required for male meiosis: targeted gene disruption in mice causes failure of meiotic progression, spermatocyte apoptosis, and male infertility, while female fertility is unaffected. HSP70-2 is associated with synaptonemal complexes in the nucleus of meiotic spermatocytes. Gene knockout (targeted disruption) in mice; immunocytology of synaptonemal complexes; nuclear fractionation Proceedings of the National Academy of Sciences of the United States of America High 8622925
1997 HSP70-2 is specifically required for synaptonemal complex desynapsis during meiotic prophase I: in Hsp70-2-/- mice, synaptonemal complexes assemble normally but fail to desynapse, and normal diplotene spermatocytes are not observed, with apoptosis beginning at the pachytene stage. Targeted gene knockout in mice; histological analysis of staged spermatogenesis; immunostaining of synaptonemal complex components; mRNA expression analysis Development (Cambridge, England) High 9409676
1997 HSP70-2 acts as a molecular chaperone for CDC2 kinase: it physically interacts with CDC2 in mouse testis, facilitates CDC2/cyclin B1 complex formation, and is required for CDC2 kinase activity. Addition of HSP70-2 to extracts from Hsp70-2-/- mice restores both complex formation and kinase activity in vitro, explaining failure at the G2/M transition. Immunoprecipitation-coupled western blot; in vitro reconstitution assay; CDC2 kinase activity assay (histone H1 phosphorylation); Hsp70-2-/- mouse testis extracts Development (Cambridge, England) High 9247342
1996 HSP70-2 is present in both the cytoplasm and nucleus of meiotic spermatocytes and is specifically associated with synaptonemal complexes at pachytene and diplotene stages in mouse and hamster; it is absent from female (oocyte) synaptonemal complexes, indicating sexual dimorphism. Two-dimensional gel electrophoresis of cytoplasmic and nuclear fractions; immunocytology of surface-spread synaptonemal complexes; RT-PCR Chromosoma High 8601336
2001 MSJ-1, a testis-specific DnaJ/Hsp40 cochaperone, physically interacts with HSP70-2 and can be co-immunoprecipitated with HSP70-2 from spermatogenic cells. MSJ-1 co-localizes with HSP70-2 in late differentiating spermatids at the acrosome and postnuclear region, suggesting a co-chaperone partnership in spermiogenesis. In vitro binding assays with recombinant MSJ-1; co-immunoprecipitation from spermatogenic cells; immunofluorescence co-localization Biology of reproduction Medium 11466217
2004 DjA4, a type I DnaJ/Hsp40 cochaperone, stimulates the ATPase activity of HSP70-2 and suppresses luciferase aggregation together with HSP70-2, indicating it functions as a cochaperone of HSP70-2. However, DjA4 (unlike DjA2) does not support efficient luciferase refolding with HSP70-2, showing functional non-equivalence between cochaperones. In vitro ATPase assay; luciferase refolding assay; luciferase aggregation suppression assay; comparison with DjA2 cochaperone Journal of biochemistry High 15047721
2006 HSPA2/HSP70-2 acquires new chaperone functions in post-meiotic spermatids, becoming tightly associated with transition proteins 1 and 2 (major spermatid DNA-packaging proteins), identifying it as the first known transition protein chaperone involved in genome condensation during spermiogenesis. Global proteomic approach (mass spectrometry) to identify genome-organizing proteins in condensing spermatids; co-purification/association studies The Journal of biological chemistry Medium 17035236
2007 HSP70-2 depletion in cancer cells triggers lysosomal membrane permeabilization and cathepsin-dependent, caspase-independent cell death. LEDGF (lens epithelium-derived growth factor) is identified as an HSP70-2-regulated downstream effector that maintains lysosomal stability; ectopic LEDGF rescues lysosomal destabilization caused by HSP70-2 knockdown. RNA interference (siRNA knockdown) of HSP70-2 in cancer cells; lysosomal membrane permeabilization assay; cathepsin release measurement; ectopic LEDGF expression rescue experiments; xenograft tumor model Cancer research Medium 17363574
2008 BAT3/Scythe is a critical regulator of HSP70-2 protein stability during spermatogenesis: Bat3 deficiency induces polyubiquitylation and proteasomal degradation of HSP70-2, depleting HSP70-2 protein despite normal transcript levels. Inhibition of proteasomal degradation restores HSP70-2 protein levels in Bat3-deficient cells. Targeted Bat3 knockout mice; western blot showing HSP70-2 protein absence with normal mRNA; ubiquitylation assay; proteasome inhibitor rescue experiment The Journal of cell biology High 18678708
1996 The Hsp70-2 promoter region between -640 and +1 (287 bp upstream of the transcription start site) is required for developmental, spermatocyte-specific expression. Transgenic promoter-reporter studies delimited the sequences sufficient to drive meiotic expression of Hsp70-2. Transgenic mice with Hsp70-2/lacZ reporter constructs of varying upstream lengths; histochemical detection of beta-galactosidase; Northern blot and in situ hybridization Developmental biology High 8631503
2004 A 165 bp fragment of the Hst70/Hsp70.2 gene promoter (containing the T1 transcription start region, exon 1, and 42 bp of the intron) is sufficient to drive testis-specific expression in transgenic mice. An Oct (octamer) sequence downstream of T1 binds repressor proteins present in juvenile rat testes when the gene is repressed. Transgenic mice with truncated promoter-CAT reporter constructs; electrophoretic mobility-shift assay (EMSA) with testis nuclear proteins The Biochemical journal Medium 14766014
2006 Human HSP70-2 promoter contains a tonicity-responsive enhancer (TonE) site at position -135 that is essential for hypertonic stress-induced transcriptional upregulation; site-directed mutagenesis of this TonE site completely abolishes hypertonicity induction, while two other TonE sites are dispensable. Luciferase reporter assays with promoter deletion constructs; site-directed mutagenesis of TonE sites; transfection into human kidney epithelial cells and fibroblasts Experimental & molecular medicine High 16819288
2009 HIF-1alpha directly binds to a hypoxia-responsive element (HRE1) at position -446 in the HSP70-2 promoter and is required for transcriptional upregulation under hypoxia. Mutation of HRE1 (but not HRE2) abolishes hypoxia-induced HSP70-2 promoter activity; HIF-1alpha siRNA or the HIF-1 inhibitor YC-1 suppresses both HIF-1alpha and HSP70-2 expression. Luciferase reporter assays with serial promoter deletions and site-directed mutagenesis; chromatin immunoprecipitation (ChIP); HIF-1alpha siRNA knockdown; HIF binding/competition assays International journal of cancer High 18844219
2008 HSF1 and HSF2 are present in epididymal spermatozoa and both bind to the Hspa1b promoter, as demonstrated by chromatin immunoprecipitation. HSF2 binding increases from early to late spermatids, suggesting that these transcription factors bookmark the Hspa1b promoter during late spermatogenesis to enable rapid expression after fertilization during minor zygotic genome activation. Western blot of spermatozoa; immunofluorescence; chromatin immunoprecipitation (ChIP) of HSF1, HSF2, and SP1 on Hspa1b promoter in spermatids and spermatozoa Biology of reproduction Medium 18434628
2009 RNA polymerase II is present in epididymal spermatozoa and bound to the Hspa1b promoter, supporting a model in which pre-loaded transcriptional machinery (HSF1, HSF2, SP1, Pol II) enables rapid Hspa1b expression after fertilization during minor zygotic genome activation. Western blot of spermatozoa; chromatin immunoprecipitation (ChIP) of RNA Pol II on Hspa1b promoter Reproduction (Cambridge, England) Medium 19336471
2016 Recombinant human HSP70-2 (Tat-Hsp70-2 fusion) exhibits ATPase activity in vitro and partially protects human neuroblastoma SH-SY5Y cells from hydrogen peroxide or 6-hydroxydopamine-induced oxidative stress when added exogenously. Recombinant protein expression in E. coli; ATPase activity assay; cell viability assay in neuroblastoma cells under oxidative stress Protein expression and purification Medium 27405095
2002 A 3'-UTR variant allele of porcine hsp70.2 gene increases mRNA half-life, as demonstrated by reporter gene assays, indicating that post-transcriptional regulation via 3'-UTR sequences contributes to variation in hsp70.2 transcript abundance. Reporter gene (luciferase) assay comparing wild-type vs. variant 3'-UTR; mRNA stability measurement Biochimica et biophysica acta Medium 12393191
2022 UBQLN2 undergoes a reversible temperature-induced conformational switch (detected by intrinsic tryptophan fluorescence) that increases its in vitro binding to HSPA1B when temperature rises from 37°C to 42°C. ALS/FTD mutant UBQLN2 proteins have an attenuated conformational switch but retain similar HSPA1B binding at 42°C. Intrinsic tryptophan fluorescence spectroscopy; in vitro protein-protein binding assay at different temperatures; comparison of WT vs. five ALS/FTD mutant UBQLN2 proteins Biochimica et biophysica acta. General subjects Medium 36423739
2004 The HSPA1B promoter polymorphism HSPA1B-179C>T is in linkage disequilibrium with HSPA1B1267A>G and is functionally associated with stimulated HSPA1A and HSPA1B mRNA levels after LPS stimulation: individuals homozygous for the C allele produce lower HSPA1A and HSPA1B mRNA than CT heterozygotes after 8 h LPS. Promoter sequencing; RT-PCR of stimulated mRNA levels in peripheral blood mononuclear cells after LPS treatment; linkage disequilibrium analysis Intensive care medicine Medium 15232679
2024 TREM-2 deletion in macrophages alleviates renal fibrosis through a pathway involving HSPa1b/AKT: TREM-2-/- macrophage exosomes suppress fibrosis, and pharmacological inhibition of HSPa1b (with VER-155008) or AKT (with LY294002) reverses this protective effect, placing HSPa1b upstream of AKT in this anti-fibrotic exosome pathway. TREM-2 knockout macrophage exosome transfer; RNA-seq; pharmacological inhibition of HSPa1b and AKT in vitro and in vivo (UUO mouse model); AAV-shTREM-2 renal pelvis injection American journal of physiology. Renal physiology Medium 39657110
2010 HSF1 regulates Hsp70.1 (Hspa1b) expression in oocytes and early embryos even in the absence of stress. HSF2 is differentially required for Hsp expression and cell survival in blastocysts under different stress conditions, establishing that HSF1 and HSF2 have distinct and complementary roles in developmental regulation of Hspa1b. HSF1 and HSF2 knockout mouse models; transgenic mice with HSE reporter; heat shock and other stress treatments of blastocysts; cell survival assays Cell stress & chaperones Medium 21053113
2010 HSP70-2 knockdown in bladder urothelial carcinoma cells (HTB-1, UMUC-3) significantly suppresses cellular motility, invasion, and tumor growth in vivo (xenograft), establishing a functional role for HSP70-2 in cancer cell migration and invasion. siRNA/shRNA knockdown of HSP70-2; colony formation assay; migration assay; invasion assay; in vivo xenograft tumor model European journal of cancer Medium 19914824
2008 HSP70-2 knockdown in hepatocellular carcinoma cells (HepG2, Huh-7) induces mitochondria-dependent apoptosis, indicated by cytochrome c release, caspase-9 and caspase-3 activation, loss of mitochondrial membrane potential, upregulation of Bax, and downregulation of Bcl-2. shRNA knockdown of HSP70-2; flow cytometry (apoptosis, mitochondrial potential); western blot for Bax, Bcl-2, PARP, caspase-9, caspase-3; cytochrome c release assay Zhonghua gan zang bing za zhi Medium 18822209
2009 miR-23a-5p directly targets the 3'-UTR of HSPA1B, as validated by dual-luciferase assay. Under hypertonic stress, miR-23a-5p is downregulated, leading to increased HSPA1B expression that promotes renal cell survival; overexpression of miR-23a-5p suppresses HSPA1B and reduces cell viability under hypertonic conditions. miRNA profiling; dual-luciferase 3'-UTR reporter assay; miR-23a-5p knockdown and overexpression; HSPA1B western blot; cell viability assay; in vivo mouse renal medulla correlation American journal of physiology. Cell physiology Medium 33206547
2009 Msx proteins (Msx1 and Msx2) activate the Hspa1b promoter via their C-terminal domains in a manner dependent on heat shock elements (HSEs); physical interactions between Msx proteins and heat shock factors may contribute to this activation. Promoter-reporter (luciferase) assays with Msx expression constructs; domain deletion analysis; HSE mutation Biochemical and biophysical research communications Low 19338779

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1996 Targeted gene disruption of Hsp70-2 results in failed meiosis, germ cell apoptosis, and male infertility. Proceedings of the National Academy of Sciences of the United States of America 450 8622925
1997 HSP70-2 is required for desynapsis of synaptonemal complexes during meiotic prophase in juvenile and adult mouse spermatocytes. Development (Cambridge, England) 194 9409676
1997 HSP70-2 is required for CDC2 kinase activity in meiosis I of mouse spermatocytes. Development (Cambridge, England) 175 9247342
1999 Role of heat shock protein HSP70-2 in spermatogenesis. Reviews of reproduction 164 10051099
2007 Lens epithelium-derived growth factor is an Hsp70-2 regulated guardian of lysosomal stability in human cancer. Cancer research 109 17363574
1993 Polymorphic analysis of the human MHC-linked heat shock protein 70 (HSP70-2) and HSP70-Hom genes in insulin-dependent diabetes mellitus (IDDM). Scandinavian journal of immunology 109 7901896
2006 Post-meiotic shifts in HSPA2/HSP70.2 chaperone activity during mouse spermatogenesis. The Journal of biological chemistry 101 17035236
1996 HSP70-2 is part of the synaptonemal complex in mouse and hamster spermatocytes. Chromosoma 85 8601336
1999 A hsp70-2 mutation recognized by CTL on a human renal cell carcinoma. Journal of immunology (Baltimore, Md. : 1950) 78 9973436
1993 Chromosomal localization of five murine HSP70 gene family members: Hsp70-1, Hsp70-2, Hsp70-3, Hsc70t, and Grp78. Genomics 77 8486356
2001 Decreased expression of the heat shock protein hsp70-2 is associated with the pathogenesis of male infertility. Fertility and sterility 71 11730740
1996 Developmentally regulated expression of Hsp70-2 and a Hsp70-2/lacZ transgene during spermatogenesis. Developmental biology 71 8631503
2007 Heat shock protein 72 (HSPA1B) gene polymorphism and Toll-like receptor (TLR) 4 mutation are associated with increased risk of urinary tract infection in children. Pediatric research 67 17314700
2010 Heat-shock protein 70-2 (HSP70-2) expression in bladder urothelial carcinoma is associated with tumour progression and promotes migration and invasion. European journal of cancer (Oxford, England : 1990) 66 19914824
2003 Heat shock protein 70 genotypes HSPA1B and HSPA1L influence cytokine concentrations and interfere with outcome after major injury. Critical care medicine 62 12544996
2003 Polymorphism of the stress protein HSP70-2 gene is associated with the susceptibility to the nasopharyngeal carcinoma. Cancer letters 60 12691826
1995 The major histocompatibility complex region marked by HSP70-1 and HSP70-2 variants is associated with clozapine-induced agranulocytosis in two different ethnic groups. Blood 59 7579351
2016 Heat shock protein 70-2 (HSP70-2) overexpression in breast cancer. Journal of experimental & clinical cancer research : CR 55 27658496
2009 Transcriptional upregulation of HSP70-2 by HIF-1 in cancer cells in response to hypoxia. International journal of cancer 55 18844219
2016 Heat shock protein 70-2 (HSP70-2) is a novel therapeutic target for colorectal cancer and is associated with tumor growth. BMC cancer 50 27473057
2005 Hsp70-2 is required for tumor cell growth and survival. Cell cycle (Georgetown, Tex.) 50 15970673
2005 Polymorphism of the TNF-alpha, HSP70-2, and CD14 genes increases susceptibility to severe acute pancreatitis. Pancreas 49 15714129
2008 Bat3 deficiency accelerates the degradation of Hsp70-2/HspA2 during spermatogenesis. The Journal of cell biology 47 18678708
1993 HLA-linked heat-shock protein 70 (HSP70-2) gene polymorphism and celiac disease. Tissue antigens 47 8096093
2005 Association of hsp70-2 and hsp-hom gene polymorphisms with risk of acute high-altitude illness in a Chinese population. Cell stress & chaperones 44 16333988
2008 Interaction of serum 70-kDa heat shock protein levels and HspA1B (+1267) gene polymorphism with disease severity in patients with chronic heart failure. Cell stress & chaperones 41 18759004
2014 Expressions of miR-15a and its target gene HSPA1B in the spermatozoa of patients with varicocele. Reproduction (Cambridge, England) 40 24481955
2005 Polymorphism of the heat-shock protein gene Hsp70-2, but not polymorphisms of the IL-10 and CD14 genes, is associated with the outcome of Crohn's disease. Scandinavian journal of gastroenterology 40 16165702
2003 HSP70-2 (HSPA1B) is associated with noncognitive symptoms in late-onset Alzheimer's disease. Journal of geriatric psychiatry and neurology 40 12967056
1995 Polymorphism of the heat-shock protein gene HSP70-2 in systemic lupus erythematosus. British journal of rheumatology 39 7551654
2001 MSJ-1, a mouse testis-specific DnaJ protein, is highly expressed in haploid male germ cells and interacts with the testis-specific heat shock protein Hsp70-2. Biology of reproduction 38 11466217
1998 HSP70-2 heat-shock protein of mouse spermatogenic cells. The Journal of experimental zoology 36 9723183
2004 The septic shock associated HSPA1B1267 polymorphism influences production of HSPA1A and HSPA1B. Intensive care medicine 34 15232679
2004 Polymorphism of stress protein HSP70-2 gene in Tunisians: susceptibility implications in type 2 diabetes and obesity. Diabetes & metabolism 33 15223990
1999 Polymorphism of heat-shock protein gene HSP70-2 in Crohn disease: possible genetic marker for two forms of Crohn disease. Scandinavian journal of gastroenterology 33 10466882
1996 The 8.5-kb PstI allele of the stress protein gene, Hsp70-2: an independent risk factor for systemic lupus erythematosus in African Americans? Human immunology 33 8655362
2010 HSFs and regulation of Hsp70.1 (Hspa1b) in oocytes and preimplantation embryos: new insights brought by transgenic and knockout mouse models. Cell stress & chaperones 32 21053113
2015 Simultaneous Administration of Dexamethasone and Vitamin E Reversed Experimental Varicocele-induced Impact in testicular tissue in Rats; Correlation with Hsp70-2 Chaperone Expression. International braz j urol : official journal of the Brazilian Society of Urology 30 26401872
2005 Polymorphisms of heat shock protein 70 gene (HSPA1A, HSPA1B and HSPA1L) and schizophrenia. Neuroscience research 30 15963589
2006 Involvement of -308 TNF-alpha and 1267 Hsp70-2 polymorphisms and zinc status in the susceptibility of coronary artery disease (CAD) in old patients. Biogerontology 29 16953332
2005 1267 HSP70-2 polymorphism as a risk factor for carotid plaque rupture and cerebral ischaemia in old type 2 diabetes-atherosclerotic patients. Mechanisms of ageing and development 29 15992611
2001 Genetic variation in the stress protein hsp70-2 gene is highly associated with obesity. International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity 29 11319647
2017 Heat shock protein 70-2 (HSP70-2) a novel cancer testis antigen that promotes growth of ovarian cancer. American journal of cancer research 28 28670489
1998 Heterogeneous patterns of constitutive and heat shock induced expression of HLA-linked HSP70-1 and HSP70-2 heat shock genes in human melanoma cell lines. Melanoma research 28 9918409
2009 Diminished reproductive potential of male mice in response to selenium-induced oxidative stress: involvement of HSP70, HSP70-2, and MSJ-1. Journal of biochemical and molecular toxicology 27 19367646
2003 Heat-shock protein HSP70-2 genotypes in patients with Crohn's disease: a more severe clinical course with intestinal complications in presence of the PstI-polymorphism. European journal of medical research 23 12730033
2009 Selenium variation induced oxidative stress regulates p53 dependent germ cell apoptosis: plausible involvement of HSP70-2. European journal of nutrition 22 19247702
2004 Modulation of chaperone activities of Hsp70 and Hsp70-2 by a mammalian DnaJ/Hsp40 homolog, DjA4. Journal of biochemistry 21 15047721
2001 Expression of Hsp70-2 in unilateral cryptorchid testis of rhesus monkey during germ cell apoptosis. Endocrine 21 11887939
1996 Sense and antisense transcripts of the developmentally regulated murine hsp70.2 gene are expressed in distinct and only partially overlapping areas in the adult brain. Brain research. Molecular brain research 21 8738139
2014 Association of heat shock protein70-2 (HSP70-2) gene polymorphism with coronary artery disease in an Iranian population. Gene 20 25108126
2002 A 3'-UTR variant of the inducible porcine hsp70.2 gene affects mRNA stability. Biochimica et biophysica acta 20 12393191
2014 Targeting the testis-specific heat-shock protein 70-2 (HSP70-2) reduces cellular growth, migration, and invasion in renal cell carcinoma cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 18 25213699
1994 HSP70-2 9.0 kb variant is in linkage disequilibrium with the HLA-B and DRB1* alleles associated with clozapine-induced agranulocytosis. The Journal of clinical psychiatry 18 7961560
2008 Interaction of HSF1 and HSF2 with the Hspa1b promoter in mouse epididymal spermatozoa. Biology of reproduction 17 18434628
2006 The role of tonicity responsive enhancer sites in the transcriptional regulation of human hsp70-2 in response to hypertonic stress. Experimental & molecular medicine 17 16819288
2014 Association between the HSPA1B ±1267A/G polymorphism and cancer risk: a meta-analysis of 14 case-control studies. Asian Pacific journal of cancer prevention : APJCP 16 25169537
1999 Completion of meiosis is not always required for acrosome formation in HSP70-2 null mice. Biology of reproduction 16 10456862
2009 RNA polymerase II interacts with the Hspa1b promoter in mouse epididymal spermatozoa. Reproduction (Cambridge, England) 15 19336471
2004 Location of promoter elements necessary and sufficient to direct testis-specific expression of the Hst70/Hsp70.2 gene. The Biochemical journal 15 14766014
2016 Association of heat shock protein70-2 (HSP70-2) gene polymorphism with obesity. Annals of human biology 14 26728972
2007 Sequence analysis of HSPA1A and HSPA1B in a multi-ethnic study population. DNA sequence : the journal of DNA sequencing and mapping 14 17364813
2006 Promoter of the heat shock testis-specific Hsp70.2/Hst70 gene is active in nervous system during embryonic development of mice. Anatomy and embryology 14 17047988
2001 Expression of HSP70-2 gene during germ cell apoptosis in rat unilateral cryptorchid testes. Archives of andrology 14 11297064
2017 Polymorphisms of heat shock protein 70 genes (HSPA1A, HSPA1B and HSPA1L) and susceptibility of noise-induced hearing loss in a Chinese population: A case-control study. PloS one 13 28182740
2008 Polymorphisms of the TNF, CD14, and HSPA1B genes in patients with acute alcohol-induced pancreatitis. Pancreas 13 18580445
1996 Distinct transcripts are recognized by sense and antisense riboprobes for a member of the murine HSP70 gene family, HSP70.2, in various reproductive tissues. Molecular reproduction and development 13 9110944
2012 Association of hsp70-2 (+1267A/G), hsp70-hom (+2437T/C), HMOX-1 (number of GT repeats) and TNF-alpha (+489G/A) polymorphisms with COPD in Croatian population. Clinical biochemistry 12 22542718
2012 Hsp70-2 gene polymorphism: susceptibility implication in Tunisian patients with coronary artery disease. Diagnostic pathology 12 22834788
2009 Analysis of Msx1 and Msx2 transactivation function in the context of the heat shock 70 (Hspa1b) gene promoter. Biochemical and biophysical research communications 12 19338779
2020 Identification of Plasmodium falciparum HSP70-2 as a resident of the Plasmodium export compartment. Heliyon 11 32529065
2019 Association of HSPA1B Polymorphisms with Paranoid Schizophrenia in a Polish Population. Neuromolecular medicine 11 31642026
2020 Inhibition of orf virus replication in goat skin fibroblast cells by the HSPA1B protein, as demonstrated by iTRAQ-based quantitative proteome analysis. Archives of virology 10 32876795
2013 Crohn's disease and polymorphism of heat shock protein gene HSP70-2 in the Chinese population. Journal of gastroenterology and hepatology 10 23425104
2000 Lack of association between the polymorphism at the heat-shock protein (HSP70-2) gene and systemic lupus erythematosus (SLE) in the Mexican mestizo population. Genes and immunity 10 11196683
1994 A pentanucleotide tandem duplication polymorphism in the 3' untranslated region of the HLA-linked heat-shock protein 70-2 (HSP70-2) gene. Human genetics 10 7959705
2019 HSPA1L and HSPA1B gene polymorphisms and haplotypes are associated with idiopathic male infertility in Iranian population. European journal of obstetrics, gynecology, and reproductive biology 9 31228677
2013 Association of HSPA1B SNP rs6457452 with Alopecia Areata in the Korean population. Immunological investigations 9 24303776
2012 Tumor cell expression of heat shock protein (HSP) 72 is influenced by HSP72 [HSPA1B A(1267)G] polymorphism and predicts survival in small Cell lung cancer (SCLC) patients. Cancer investigation 9 22468780
2007 Crohn's disease and polymorphism of heat shock protein gene HSP70-2 in the Tunisian population. European journal of gastroenterology & hepatology 9 17301649
2020 A novel tonicity-responsive microRNA miR-23a-5p modulates renal cell survival under osmotic stress through targeting heat shock protein 70 HSPA1B. American journal of physiology. Cell physiology 8 33206547
2019 Increase of Hspa1a and Hspa1b genes in the resting B cells of Sirt1 knockout mice. Molecular biology reports 8 31102152
2008 Polymorphisms of the MCP-1 and HSP70-2 genes in Korean patients with alcoholic chronic pancreatitis. Digestive diseases and sciences 8 17940904
2002 Expression of Hsp70-2 in rhesus monkey testis during germ cell apoptosis induced by testosterone undecanoate. Contraception 8 12443970
2020 Genetic association study of a novel indel polymorphism in HSPA1B with the risk of sudden cardiac death in the Chinese populations. Forensic science international 7 33309992
2009 [Hypoxia induces heat shock protein HSP70-2 expression in a HIF-1 dependent manner]. Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology 7 19335985
2024 Exosomes derived from TREM-2 knocked-out macrophages alleviated renal fibrosis via HSPa1b/AKT pathway. American journal of physiology. Renal physiology 6 39657110
2015 Mutations in HSP70-2 gene change the susceptibility to clinical mastitis in Chinese Holstein. Gene 6 25592821
2008 [Inhibition of HSP70-2 expression by RNA interference induces apoptosis of human hepatocellular carcinoma cells]. Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology 6 18822209
2022 Next Generation Sequencing of Genotype Variants and Genetic Association between Heat Shock Proteins HSPA1B Single Nucleotide Polymorphism at the g.31829044 Locus and Heat Tolerance: A Pilot Quasi-Experimental Study. Biomolecules 5 36291674
2022 UBQLN2 undergoes a reversible temperature-induced conformational switch that regulates binding with HSPA1B: ALS/FTD mutations cripple the switch but do not destroy HSPA1B binding. Biochimica et biophysica acta. General subjects 5 36423739
2016 Recombinant human Tat-Hsp70-2: A tool for neuroprotection. Protein expression and purification 5 27405095
2015 Genetic polymorphisms of hspa1b and hspa1l in infertile men. JPMA. The Journal of the Pakistan Medical Association 5 26160076
2006 Polymorphisms of heat shock protein-70 (HSPA1B and HSPA1L loci) do not influence infection or outcome risk in critically ill surgical patients. Shock (Augusta, Ga.) 5 16525348
1998 Analysis of the 5'-flanking regions of the MHC-linked Hsp70-2 and Hsp70-3 genes of the rat. Biochimica et biophysica acta 5 9434152
2017 [The GG genotype of the HSPA1B gene is associated with increased risk of glaucoma in northern Iran]. Molekuliarnaia biologiia 4 28251963
2025 The Endothelial Cell-Related Genes EIF1 and HSPA1B Contribute to the Pathogenesis of Alzheimer's Disease by Modulating Peripheral Immunoinflammatory Responses. Brain sciences 3 40002537
2020 Erratum: Heat shock protein 70-2 (HSP70-2) a novel cancer testis antigen that promotes growth of ovarian cancer. American journal of cancer research 3 32642300
1999 Heat-shock protein (HSP70-2) allelic frequencies in three distinct Mexican populations. Genes and immunity 3 11197308
2022 Up-regulation of HSPA1A and HSPA1B in the blood of tophi patients and its clinical significance. Acta biochimica Polonica 2 36515646

Missed literature

Know a paper Affinage missed for HSPA1B? Flag it for the maintainers and the community.

No submissions yet.