Affinage

Showing CBX8 — HPC3 is a alias.

CBX8

Chromobox protein homolog 8 · UniProt Q9HC52

Length
389 aa
Mass
43.4 kDa
Annotated
2026-06-09
62 papers in source corpus 27 papers cited in narrative 27 extracted findings
Cross-family judge vs UniProt: tie faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CBX8 is a Polycomb group chromodomain protein that functions as a context-dependent epigenetic regulator of senescence, proliferation, differentiation, DNA repair, and cancer progression (PMID:17332741, PMID:27505670). It was first defined as a PcG protein that interacts with RING1 via its C-box, localizes to PcG bodies, and acts as a long-range transcriptional silencer (PMID:10825164), and as a PRC1 subunit it directly binds and represses the INK4A-ARF locus to bypass senescence and immortalize cells (PMID:17332741). Its chromodomain drives chromatin association through dual binding to H3K27me3 and DNA (PMID:30597065), and at the structural level PRC1-CBX8 condenses chromatin into a porous, accessible state in which positively charged residues on CBX8's intrinsically disordered regions mask DNA negative charges to stabilize the condensate rather than rigidly compact it (PMID:39815045). In its canonical repressive mode CBX8 occupies target promoters to establish H2AK119ub1 and maintain H3K27me3 — silencing CDKN2C, SCEL, SUSD2, WNK2, Snail, PRDM1, and DDIT4 — often by recruiting co-repressors including SET, BCOR, and TRIM28 (PMID:35894945, PMID:37733753, PMID:33731128, PMID:41943835), and assembling non-canonical PRC1-BCOR complexes at bivalent promoters together with EZH2/BCL6 (PMID:27505670). In non-canonical activating contexts CBX8 instead associates with WDR5- and KMT2b-containing H3K4 methyltransferase complexes to maintain H3K4me3 and promote transcription of Notch-network and LGR5 genes (PMID:27346354, PMID:31849331), and it can act through transcription-factor and microRNA circuits, as in MLL-AF9/TIP60-driven HOX activation and leukemogenesis (PMID:22094252). CBX8 protein level and complex integrity are tuned by phosphorylation: PIM1 (S196), PKD1 (T234, S256/311), and PLK1 (S265) variously promote its degradation or disrupt PRC1 binding, while TRIM28 stabilizes it and NSUN5-mediated m5C methylation of CBX8 mRNA increases its abundance (PMID:29763603, PMID:34253692, PMID:41398073, PMID:40240371, PMID:42066830). CBX8 also functions outside transcription, accumulating at DNA-damage sites in a PARP1- and TRIM33-dependent manner to support recombination repair (PMID:27555324) and acting as an RNA-binding protein that retains pre-miR-378a-3p in the nucleus to limit its maturation (PMID:33417156).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 2000 Medium

    Established CBX8 as a bona fide Polycomb group protein with intrinsic silencing capacity, defining the framework for all later mechanistic work.

    Evidence Yeast two-hybrid and co-IP showing RING1 interaction via the C-box, PcG-body co-localization, and a tethered transcriptional reporter

    PMID:10825164

    Open questions at the time
    • No direct target genes identified
    • Chromatin-binding determinants not yet defined
  2. 2007 High

    Showed CBX8-PRC1 directly represses a defined locus with a physiological consequence, linking CBX8 to senescence control and oncogenic immortalization.

    Evidence ChIP at INK4A-ARF plus gain/loss-of-function senescence and immortalization assays in human and mouse fibroblasts

    PMID:17332741

    Open questions at the time
    • Mechanism of locus selection not resolved
    • Did not address non-canonical activating roles
  3. 2011 High

    Demonstrated that CBX8 can act as a transcriptional activator in an oncogenic context, revealing a function beyond canonical Polycomb silencing.

    Evidence Reciprocal Co-IP with MLL-AF9 and TIP60, interface point mutagenesis, Cbx8 knockout mice, and leukemia transformation assays

    PMID:22094252

    Open questions at the time
    • Chromatin modifications underlying activation not defined here
    • Whether activation requires the chromodomain unresolved at this stage
  4. 2014 Medium

    Generalized the activator role to development, showing Cbx7-to-Cbx8 PRC1 subunit exchange is needed to activate differentiation genes.

    Evidence ChIP-seq, expression microarray, co-IP, and siRNA depletion in mouse ES cell differentiation

    PMID:25500566

    Open questions at the time
    • Mechanism converting PRC1 from repressor to activator unclear
    • Direct vs indirect activation not distinguished
  5. 2016 High

    Defined distinct partner-dependent modes: a non-canonical PRC1-BCOR repressive complex versus a WDR5-associated activating complex, plus a SIRT1 senescence-suppression axis.

    Evidence Co-IP/ChIP of PRC1-BCOR-CBX8 in GC B cells; loss-of-function screen, Co-IP and ChIP-seq for WDR5/Notch; Co-IP/GST pulldown for SIRT1

    PMID:23474493 PMID:27346354 PMID:27505670

    Open questions at the time
    • Determinants of complex choice at a given promoter unknown
    • Stoichiometry of mutually exclusive complexes not measured
  6. 2016 Medium

    Revealed a transcription-independent genome-protective role for CBX8 in DNA double-strand break repair.

    Evidence Laser microirradiation live imaging, Co-IP with TRIM33, siRNA knockdown, and HR/NHEJ and radiosensitivity assays

    PMID:27555324

    Open questions at the time
    • Molecular substrate of CBX8 at break sites unknown
    • Whether PRC1 is required at damage sites not established
  7. 2019 High

    Provided the structural basis for CBX8 chromatin engagement and a chemical probe demonstrating chromodomain function is druggable and required for MLL-AF9 target activation.

    Evidence Structural/biochemical characterization of chromodomain dual H3K27me3-DNA binding; DNA-encoded library inhibitor SW2_110A with cellular chromatin displacement and proliferation assays

    PMID:30597065 PMID:31755685

    Open questions at the time
    • Relative contribution of DNA vs histone binding in vivo not quantified
    • Probe selectivity across CBX paralogs in cells limited
  8. 2019 Medium

    Expanded the non-canonical activation mechanism, showing CBX8 recruits KMT2b to maintain H3K4me3 at the LGR5 promoter and that CBX8 mRNA is stabilized by methylation.

    Evidence ChIP-seq, RNA-seq, Co-IP, luciferase reporter, RIP, and sphere-formation assays

    PMID:31849331

    Open questions at the time
    • Switch between KMT2b-activating and PRC1-repressive states unresolved
    • RNA methylation enzyme not identified here
  9. 2021 Medium

    Defined post-translational and import controls of CBX8 abundance and localization, and uncovered an RNA-binding function regulating microRNA maturation.

    Evidence CRISPR screen, Co-IP/MS for PIM1 and TRIM28 stability control; KPNA2 nuclear-import Co-IP and BCOR/PRDM1 ChIP; RIP plus RNA-binding-domain and NLS mutagenesis for pre-miR-378a-3p retention

    PMID:33417156 PMID:33731128 PMID:34253692

    Open questions at the time
    • Kinase that drives S196 phosphorylation in other contexts not mapped
    • Generality of CBX8 RNA binding beyond miR-378a unknown
  10. 2022 Medium

    Mechanistically detailed CBX8-driven repression through co-repressor recruitment and H2AK119ub1 deposition at tumor-suppressor promoters.

    Evidence FLAG affinity purification/MS identifying SET, Co-IP, ChIP, and RNA-seq rescue at the SUSD2 promoter

    PMID:35894945

    Open questions at the time
    • E3 ligase activity responsible for H2AK119ub1 in this complex not isolated
    • INHAT acetylation-blocking step not directly demonstrated
  11. 2025 High

    Established phosphorylation as a master switch over CBX8: kinases control its degradation, PRC1 complex integrity, and downstream activation outcomes.

    Evidence In vitro kinase assays with site mutagenesis for PKD1 (T234, S256/311) and PLK1 (S265), Co-IP for BMI1/RING1A binding, ChIP, ubiquitination, and ferroptosis/senescence readouts in cells and PDX models

    PMID:40240371 PMID:41398073

    Open questions at the time
    • Phosphatases reversing these marks not identified
    • Cross-talk among the multiple phospho-sites not integrated
  12. 2025 High

    Provided a structural mechanism showing PRC1-CBX8 stabilizes accessible, porous chromatin condensates through IDR-DNA charge masking rather than compaction.

    Evidence Cryo-electron tomography, in vitro chromatin condensation reconstitution, ATAC-seq accessibility, and IDR mutagenesis

    PMID:39815045

    Open questions at the time
    • How condensate accessibility reconciles with gene silencing not fully explained
    • In vivo relevance of static-chromatin/dynamic-PRC1 model needs broader testing
  13. 2026 Medium

    Linked CBX8 to mTOR and JAK-STAT signaling via co-repressor recruitment and to an upstream RNA-modification control of its own abundance.

    Evidence ChIP/Co-IP of TRIM28 at DDIT4 with H3K27me3 readout; NSUN5 catalytic-mutant rescue and RIP for m5C-dependent CBX8 mRNA stabilization, with inhibitor and in vivo validation

    PMID:41943835 PMID:42066830

    Open questions at the time
    • Whether m5C and phospho-control act jointly on CBX8 levels untested
    • Tissue specificity of the SOCS2/JAK-STAT axis unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unresolved what determines, at a given locus, whether CBX8 assembles a repressive PRC1/co-repressor complex versus an activating WDR5/KMT2b complex, and how its condensate, phospho-, and RNA-binding states are integrated.
  • No unified model linking complex choice to chromatin state
  • Phosphatases and upstream signals governing the phospho-switch unidentified
  • Generality of RNA-binding and DNA-repair roles relative to transcriptional roles unclear

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 7 GO:0060090 molecular adaptor activity 4 GO:0003677 DNA binding 2 GO:0042393 histone binding 2 GO:0003723 RNA binding 1
Localization
GO:0000228 nuclear chromosome 3 GO:0005634 nucleus 3 GO:0005654 nucleoplasm 2
Pathway
R-HSA-1643685 Disease 6 R-HSA-74160 Gene expression (Transcription) 6 R-HSA-4839726 Chromatin organization 3 R-HSA-1266738 Developmental Biology 1 R-HSA-73894 DNA Repair 1
Partners
BCORBMI1KMT2BRING1SETSIRT1TRIM28WDR5
Complex memberships
PRC1WDR5/KMT2b (MLL4) H3K4 methyltransferase complexnon-canonical PRC1-BCOR-CBX8

Evidence

Reading pass · 27 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 CBX8 (HPC3) was identified as a novel Polycomb group protein that physically interacts with RING1 via its C-box domain; this interaction occurs in vivo preferentially with covalently modified forms of RING1. CBX8 co-localizes with other PcG proteins in PcG bodies and functions as a long-range transcriptional silencer when tethered to a reporter gene. Yeast two-hybrid screen, co-immunoprecipitation, fluorescence microscopy (co-localization), transcriptional reporter assay The Journal of biological chemistry Medium 10825164
2007 CBX8, as a component of a PRC1 complex, directly binds the INK4A-ARF locus and represses it; ectopic CBX8 expression leads to Ink4a-Arf repression, bypass of senescence, and cellular immortalization in human and mouse diploid fibroblasts. Chromatin immunoprecipitation (ChIP), gene expression analysis, ectopic overexpression and loss-of-function in primary fibroblasts, genome-wide location analysis The EMBO journal High 17332741
2011 CBX8 physically interacts with MLL-AF9 and TIP60, and is required for MLL-AF9-mediated transcriptional activation of HOX genes and leukemic transformation; point mutations disrupting the CBX8–MLL-AF9 interaction abolish HOX upregulation and leukemogenesis. Co-immunoprecipitation, point mutagenesis of MLL-AF9, Cbx8 knockout mice, leukemia transformation assays in vivo and in vitro Cancer cell High 22094252
2013 CBX8 physically interacts with SIRT1 and cooperates with it to suppress p53 acetylation and p21WAF1 expression, thereby repressing premature senescence in breast cancer cells. CBX8 prevents SIRT1-inhibitor-induced senescence, and this effect is reversed by CBX8 knockdown. Co-immunoprecipitation, GST pull-down, fluorescence microscopy, transcriptional repression reporter, siRNA knockdown, Western blot Cancer letters Medium 23474493
2014 A Cbx8-containing PRC1 complex facilitates transcriptional activation of developmental genes during ES cell differentiation; exchange of Cbx7 for Cbx8 in the PRC1 complex is required for effective activation of differentiation genes. Depletion of Cbx8 partially impairs activation of these genes. Activating Cbx8 associates with intact PRC1 components as shown by ChIP. ChIP-seq (genome-wide), gene expression microarray, interaction analysis (co-immunoprecipitation), siRNA depletion in mouse ES cells PLoS genetics Medium 25500566
2016 EZH2 and BCL6 cooperate to assemble a non-canonical PRC1-BCOR-CBX8 complex at bivalent promoters in germinal center B cells; CBX8 binds H3K27me3 at these promoters and is required for stable complex association and for the resulting histone modifications, GC formation, and lymphomagenesis. Co-immunoprecipitation, ChIP, CBX8 inducible expression in GC B cells and lymphoma cells, mouse GC formation assays Cancer cell High 27505670
2016 Cbx8 associates with non-PRC1 complexes containing WDR5, a component of H3K4 methyltransferase complexes, and together they maintain H3K4me3 levels on Notch-network gene promoters, positively regulating Notch signaling to promote mammary tumorigenesis. Loss-of-function screen, co-immunoprecipitation, ChIP-seq, Notch1 rescue experiment, in vivo tumorsphere assays Cell reports Medium 27346354
2016 CBX8 rapidly accumulates at sites of DNA damage within 30 seconds in a PARP1-dependent (but ATM-independent) manner. CBX8 biochemically interacts with TRIM33, and its recruitment to DNA damage sites requires TRIM33. CBX8 knockdown reduces efficiency of both homologous and non-homologous recombination and increases cellular sensitivity to ionizing radiation. Live-cell imaging (laser microirradiation), co-immunoprecipitation, siRNA knockdown, DNA repair assays, ionizing radiation sensitivity assay The Journal of biological chemistry Medium 27555324
2017 CBX8 activates AKT/β-catenin signaling in hepatocellular carcinoma via a non-canonical mechanism: it directly binds the EGR1 promoter to enhance its transcription and also interacts with EGR1 protein in the nucleus to prevent its degradation; additionally, CBX8 increases miR-365a-3p transcription, which promotes nuclear localization of β-catenin by targeting ZNRF1 3'-UTR. ChIP, co-immunoprecipitation, luciferase reporter assay, Western blot, ectopic expression and knockdown, in vivo tumor assays Cancer research Medium 29066512
2017 CBX8 suppresses ESCC metastasis by directly binding the Snail promoter to repress Snail transcription, thereby inhibiting EMT. ChIP, luciferase reporter, siRNA knockdown, in vitro migration/invasion assays, in vivo metastasis assay Theranostics Medium 28912889
2018 PIM1 kinase phosphorylates CBX8, promoting its proteasomal degradation, which leads to upregulation of p16 and induction of oncogene-induced senescence in human diploid fibroblasts. In vitro kinase assay, overexpression and knockdown of PIM1/CBX8, Western blot, senescence assays Biochemical and biophysical research communications Medium 29763603
2019 The CBX8 chromodomain (CD) drives chromatin association through dual binding to H3K27me3 and DNA; structural characterization revealed integration of both activities and showed that the chromatin environment is critical for determining CBX8 CD function in chromatin association. In vitro binding assays, mutagenesis, structural characterization (NMR/biochemical), cellular chromatin association assays (ChIP), fluorescence polarization Nucleic acids research High 30597065
2019 CBX8 chromodomain inhibitor SW2_110A (Kd ~800 nM) selectively displaces CBX8 from chromatin in cells in a chromodomain-dependent manner, reducing MLL-AF9 target gene expression (including HOXA9) and inhibiting THP1 leukemia cell proliferation, establishing the chromodomain as necessary for CBX8's role in MLL-AF9 transcriptional activation. DNA-encoded library selection, fluorescence polarization, cellular chromatin displacement assay, gene expression analysis, cell proliferation assay ACS chemical biology Medium 31755685
2019 CBX8 upregulates LGR5 expression in a non-canonical manner by recruiting KMT2b (MLL4) to the LGR5 promoter, maintaining H3K4me3 to promote LGR5 transcription, thereby promoting cancer stemness; CBX8 mRNA stability is maintained by m6A methylation. ChIP-seq, RNA-seq, ChIP, co-immunoprecipitation, luciferase reporter assay, RNA immunoprecipitation, sphere formation assay Molecular cancer Medium 31849331
2019 CBX8 interacts with YBX1; YBX1 knockdown impairs CBX8-mediated upregulation of CyclinD1 and cell proliferation in hepatocellular carcinoma cells, establishing a CBX8-YBX1-CyclinD1 axis in cell cycle regulation. Co-immunoprecipitation, bioinformatics, siRNA knockdown, Western blot, proliferation assays Aging Low 31495785
2020 CBX8 binds the WNK2 promoter to suppress WNK2 expression, resulting in elevated MMP2 and RAC1 activity, thereby promoting invasion and migration in glioblastoma, breast cancer, and lung cancer cells. ChIP, luciferase reporter, ectopic expression and knockdown, invasion/migration assays in vitro and in vivo Molecular therapy oncolytics Medium 33251331
2021 PIM1 phosphorylates CBX8 at serine 196, promoting its proteasomal degradation during erythroid differentiation of K562 CML cells; TRIM28 interacts with CBX8 and maintains its protein stability, and TRIM28 loss induces proteasomal degradation of CBX8 and accelerates erythroid differentiation. CRISPR-Cas9 screen, co-immunoprecipitation, proteomic analysis (mass spectrometry), siRNA knockdown, proteasome inhibitor treatment, Western blot, flow cytometry (CD235A) Molecules and cells Medium 34253692
2021 KPNA2 promotes nuclear import of CBX8; CBX8 in turn downregulates PRDM1 by recruiting BCOR to the PRDM1 promoter region in bladder cancer cells. Co-immunoprecipitation, chromatin immunoprecipitation (ChIP), nuclear fractionation, knockdown/overexpression, in vivo xenograft model Journal of translational medicine Medium 33731128
2021 CBX8 acts as an RNA-binding protein that interacts with pre-miR-378a-3p via its RNA-binding domain and retains it in the nucleus, limiting nucleoplasmic transport and thereby inhibiting maturation of miR-378a-3p; mutation of the RNA-binding domain or nuclear localization signal of CBX8 abolishes this regulation. RNA immunoprecipitation, mutagenesis of RNA-binding domain and NLS, miRNA expression profiling, nucleocytoplasmic fractionation Human cell Medium 33417156
2021 CBX8 positive allosteric modulator UNC7040 antagonizes H3K27me3 binding by CBX8 while increasing its interactions with nucleic acids, leading to eviction of CBX8-containing PRC1 from chromatin, loss of gene silencing, and reduced cancer cell proliferation. Quantitative cellular assay, in vitro biochemical binding assay, ChIP, gene expression analysis, cell proliferation assay Cell chemical biology Medium 34715055
2022 CBX8 interacts with SET (an INHAT subunit) and together they co-bind the SUSD2 promoter to establish H2AK119ub1 and prevent histone H3 acetylation, resulting in transcriptional suppression of the tumor suppressor SUSD2 in ovarian carcinoma cells. FLAG affinity purification coupled with mass spectrometry, co-immunoprecipitation, ChIP, RNA-seq, functional rescue experiments Molecular cancer research : MCR Medium 35894945
2023 CBX8 directly binds the promoters of CDKN2C and SCEL to establish H2AK119ub1, repressing their transcription and thereby promoting lung adenocarcinoma growth and metastasis; CBX8 depletion reduces H2AK119ub1 at these promoters and restores their expression. ChIP, RNA-seq, RT-PCR, knockdown and rescue experiments in cell culture and mouse models Journal of cellular physiology Medium 37733753
2025 PKD1 (protein kinase D1) physically interacts with CBX8 and phosphorylates it at Thr234 and Ser256/311; phosphorylation at Thr234 promotes ubiquitination-mediated degradation of CBX8, while Ser256/311 phosphorylation decreases CBX8 binding to PRC1 components BMI1 and RING1A, impairing PRC1 complex integrity, reducing H2AK119ub1, and derepressing p16INK4A to facilitate cellular senescence. In vitro kinase assay, site-directed mutagenesis, co-immunoprecipitation, Western blot, ubiquitination assay, ChIP, gene expression analysis Oncogene High 41398073
2025 PLK1 phosphorylates CBX8 at Ser265, driving GPX4 expression, which antagonizes BRAFi±EGFRi-induced ferroptosis in BRAFV600E colorectal cancer; disruption of this PLK1-CBX8-GPX4 axis by PLK1 inhibition enhances ferroptosis and overcomes drug resistance. Genome-wide CRISPR-Cas9 screen, in vitro kinase assay (PLK1), site mutagenesis, ChIP, Western blot, ferroptosis assays, organoid and PDX models Nature communications High 40240371
2025 Cryo-electron tomography revealed that PRC1-CBX8-condensed chromatin forms a porous, accessible structure stabilized by multivalent dynamic interactions. Mechanistically, positively charged residues on the intrinsically disordered regions (IDRs) of CBX8 mask negative charges on DNA to stabilize condensed chromatin; PRC1 remains dynamic within condensates while the chromatin structure is static. In differentiated mouse ES cells, CBX8-bound chromatin remains accessible. Cryo-electron tomography (3D structural determination), in vitro chromatin condensation reconstitution, accessibility assays (ATAC-seq), mutagenesis Nature structural & molecular biology High 39815045
2026 CBX8 inhibits autophagy-dependent senescence in colorectal cancer by transcriptionally repressing DDIT4 (a negative regulator of mTOR); CBX8 recruits TRIM28 to the DDIT4 promoter, maintaining H3K27me3 and repressing DDIT4 expression, thereby activating mTOR signaling. ChIP, co-immunoprecipitation, RNA-seq, CBX8 knockdown and overexpression in vitro and in vivo, mTOR signaling readouts, senescence assays International journal of biological sciences Medium 41943835
2026 NSUN5 methylates CBX8 mRNA (m5C), stabilizing it and increasing CBX8 protein levels; elevated CBX8 then represses SOCS2 expression as part of PRC1, activating JAK-STAT3 signaling to promote gastric tumorigenesis. Catalytic mutant rescue (Nsun5C330A/C404A), RNA immunoprecipitation, Western blot, CBX8 inhibitor (SW2-110A) functional experiments, in vivo mouse tumor model Cellular signalling Medium 42066830

Source papers

Stage 0 corpus · 62 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1998 A SWI/SNF-related chromatin remodeling complex, E-RC1, is required for tissue-specific transcriptional regulation by EKLF in vitro. Cell 265 9778250
2016 EZH2 and BCL6 Cooperate to Assemble CBX8-BCOR Complex to Repress Bivalent Promoters, Mediate Germinal Center Formation and Lymphomagenesis. Cancer cell 201 27505670
2007 Bypass of senescence by the polycomb group protein CBX8 through direct binding to the INK4A-ARF locus. The EMBO journal 160 17332741
2011 CBX8, a polycomb group protein, is essential for MLL-AF9-induced leukemogenesis. Cancer cell 137 22094252
1996 SMC proteins constitute two subunits of the mammalian recombination complex RC-1. The EMBO journal 115 8670910
2019 m6A modification-mediated CBX8 induction regulates stemness and chemosensitivity of colon cancer via upregulation of LGR5. Molecular cancer 108 31849331
2016 Cbx8 Acts Non-canonically with Wdr5 to Promote Mammary Tumorigenesis. Cell reports 82 27346354
2018 CircRNA8924 Promotes Cervical Cancer Cell Proliferation, Migration and Invasion by Competitively Binding to MiR-518d-5p /519-5p Family and Modulating the Expression of CBX8. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 80 30007986
2017 CBX8 Exhibits Oncogenic Activity via AKT/β-Catenin Activation in Hepatocellular Carcinoma. Cancer research 72 29066512
2019 Optimization of Ligands Using Focused DNA-Encoded Libraries To Develop a Selective, Cell-Permeable CBX8 Chromodomain Inhibitor. ACS chemical biology 61 31755685
2000 HPC3 is a new human polycomb orthologue that interacts and associates with RING1 and Bmi1 and has transcriptional repression properties. The Journal of biological chemistry 60 10825164
2014 A Cbx8-containing polycomb complex facilitates the transition to gene activation during ES cell differentiation. PLoS genetics 56 25500566
2014 Paradoxical role of CBX8 in proliferation and metastasis of colorectal cancer. Oncotarget 52 25360999
2007 A novel bubble liposome and ultrasound-mediated gene transfer to ocular surface: RC-1 cells in vitro and conjunctiva in vivo. Experimental eye research 47 17889849
2018 Robustness of In Vitro Selection Assays of DNA-Encoded Peptidomimetic Ligands to CBX7 and CBX8. SLAS discovery : advancing life sciences R & D 44 29309209
2017 CBX8 Suppresses Tumor Metastasis via Repressing Snail in Esophageal Squamous Cell Carcinoma. Theranostics 43 28912889
2013 CBX8 suppresses Sirtinol-induced premature senescence in human breast cancer cells via cooperation with SIRT1. Cancer letters 40 23474493
1999 Cloning and characterization of mammalian SMC1 and SMC3 genes and proteins, components of the DNA recombination complexes RC-1. Gene 39 10072753
2019 Engagement of DNA and H3K27me3 by the CBX8 chromodomain drives chromatin association. Nucleic acids research 33 30597065
1992 cDNA cloning of rat proteasome subunit RC1, a homologue of RING10 located in the human MHC class II region. FEBS letters 30 1451788
2022 Quorum Sensing Inhibitory and Quenching Activity of Bacillus cereus RC1 Extracts on Soft Rot-Causing Bacteria Lelliottia amnigena. ACS omega 28 35910130
2022 Actinomycin D: a novel Pseudomonas aeruginosa quorum sensing inhibitor from the endophyte Streptomyces cyaneochromogenes RC1. World journal of microbiology & biotechnology 26 35904625
2021 KPNA2 interaction with CBX8 contributes to the development and progression of bladder cancer by mediating the PRDM1/c-FOS pathway. Journal of translational medicine 25 33731128
1996 Analysis of the mammalian recombination protein complex RC-1. Mutation research 25 8657184
2021 Reprogramming CBX8-PRC1 function with a positive allosteric modulator. Cell chemical biology 24 34715055
2020 Upregulated CBX8 Promotes Cancer Metastasis via the WNK2/MMP2 Pathway. Molecular therapy oncolytics 24 33251331
2014 CBX8, a novel DNA repair protein, promotes tumorigenesis in human esophageal carcinoma. International journal of clinical and experimental pathology 24 25197352
2019 Chromobox homolog 8 (CBX8) Interacts with Y-Box binding protein 1 (YBX1) to promote cellular proliferation in hepatocellular carcinoma cells. Aging 21 31495785
2015 CBX8 antagonizes the effect of Sirtinol on premature senescence through the AKT-RB-E2F1 pathway in K562 leukemia cells. Biochemical and biophysical research communications 20 26718407
2019 CBX8 promotes tumorigenesis and confers radioresistance in esophageal squamous cell carcinoma cells through targeting APAF1. Gene 18 31255735
2021 Enhanced cadmium removal by growing Bacillus cereus RC-1 immobilized on different magnetic biochars through simultaneous adsorption and bioaccumulation. Environmental science and pollution research international 16 34689298
2016 A Novel Role of Chromodomain Protein CBX8 in DNA Damage Response. The Journal of biological chemistry 16 27555324
2015 Insulin-Like Growth Factor-1 Modulates Polycomb Cbx8 Expression and Inhibits Colon Cancer Cell Apoptosis. Cell biochemistry and biophysics 16 25398592
2025 Dynamic PRC1-CBX8 stabilizes a porous structure of chromatin condensates. Nature structural & molecular biology 14 39815045
2025 Targeting PLK1-CBX8-GPX4 axis overcomes BRAF/EGFR inhibitor resistance in BRAFV600E colorectal cancer via ferroptosis. Nature communications 14 40240371
2016 l-2-Haloacid dehalogenase (DehL) from Rhizobium sp. RC1. SpringerPlus 13 27347470
2022 CBX8 Together with SET Facilitates Ovarian Carcinoma Growth and Metastasis by Suppressing the Transcription of SUSD2. Molecular cancer research : MCR 12 35894945
1979 The amplitude of post-tetanic potentiation of the EPSP RC1-R15 in Aplysia is modulated by environmental parameters. Brain research 12 226212
2021 Polycomb Paralog Chromodomain Inhibitors Active against Both CBX6 and CBX8*. ChemMedChem 11 34174168
2016 Multi-template homology-based structural model of L-2-haloacid dehalogenase (DehL) from Rhizobium sp. RC1. Journal of biomolecular structure & dynamics 10 27800712
2023 CBX8 promotes lung adenocarcinoma growth and metastasis through transcriptional repression of CDKN2C and SCEL. Journal of cellular physiology 9 37733753
2018 PIM1-catalyzed CBX8 phosphorylation promotes the oncogene-induced senescence of human diploid fibroblast. Biochemical and biophysical research communications 9 29763603
2017 Deciphering the catalytic amino acid residues of l-2-haloacid dehalogenase (DehL) from Rhizobium sp. RC1: An in silico analysis. Computational biology and chemistry 9 28873365
2023 TRIM28 recruits E2F1 to regulate CBX8-mediated cell proliferation and tumor metastasis of ovarian cancer. Human cell 8 37709991
2021 Genome-wide CRISPR-Cas9 screening identifies that hypoxia-inducible factor-1a-induced CBX8 transcription promotes pancreatic cancer progression via IRS1/AKT axis. World journal of gastrointestinal oncology 8 34853645
2014 Insights into the stereospecificity of the d-specific dehalogenase from Rhizobium sp. RC1 toward d- and l-2-chloropropionate. Biotechnology, biotechnological equipment 8 26740767
2021 CBX8 acts as an independent RNA-binding protein to regulate the maturation of miR-378a-3p in colon cancer cells. Human cell 7 33417156
2021 hsa‑miR‑429 targets CBX8 to promote cell apoptosis in diffuse large B‑cell lymphoma. Molecular medicine reports 7 34651663
2015 Identification of functional residues essential for dehalogenation by the non-stereospecific α-haloalkanoic acid dehalogenase from Rhizobium sp. RC1. Journal of basic microbiology 7 25727054
2021 Negative Regulation of Erythroid Differentiation via the CBX8-TRIM28 Axis. Molecules and cells 6 34253692
2021 CBX8 interacts with chromatin PTEN and is involved in regulating mitotic progression. Cell proliferation 6 34592789
2012 Structure prediction, molecular dynamics simulation and docking studies of D-specific dehalogenase from Rhizobium sp. RC1. International journal of molecular sciences 5 23443090
2022 Cloning and characterization of Aiia, an acylhomoserine lactonase from Bacillus cereus RC1 to control soft rot causing pathogen Lelliottia amnigena RCE. Archives of microbiology 4 36209456
2019 Theoretical analyses on enantiospecificity of L-2-haloacid dehalogenase (DehL) from Rhizobium sp. RC1 towards 2-chloropropionic acid. Journal of molecular graphics & modelling 4 31352207
2024 CBX8 Promotes Epithelial-mesenchymal Transition, Migration, and Invasion of Lung Cancer through Wnt/β-catenin Signaling Pathway. Current protein & peptide science 2 38265409
2024 Dynamic PRC1-CBX8 stabilizes a porous structure of chromatin condensates. bioRxiv : the preprint server for biology 1 38405976
2026 CBX8 suppresses autophagy-dependent senescence in colorectal cancer by modulating the mTOR signaling pathway. International journal of biological sciences 0 41943835
2026 NSUN5-CBX8 m5C axis promotes gastric tumorigenesis by activating JAK-STAT3 signaling. Cellular signalling 0 42066830
2025 RETRACTION: CBX8 Interacts with Chromatin PTEN and Is Involved in Regulating Mitotic Progression. Cell proliferation 0 40538052
2025 Phosphorylation of CBX8 by PKD1 suppresses PRC1 activity and promotes cell senescence. Oncogene 0 41398073
2023 CBX8 Promotes Cell Proliferation and Metastasis and Leads to Radiotherapy Tolerance of Glioma Cells. Turkish neurosurgery 0 35713250
1982 Modulation of synapse RC1-R15 of Aplysia californica by fiber(s) of the right connective. Canadian journal of physiology and pharmacology 0 7104855

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