Affinage

H1-0

Histone H1.0 · UniProt P07305

Length
194 aa
Mass
20.9 kDa
Annotated
2026-06-10
100 papers in source corpus 32 papers cited in narrative 32 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

H1-0 is a linker histone that binds the nucleosome and compacts chromatin to repress transcription, thereby restricting cellular plasticity and driving terminal differentiation (PMID:27708074, PMID:2579343). Its globular domain engages the nucleosome through two distinct DNA-binding sites—one at the major groove near the dyad and one on linker DNA—clustering multiple residues to form the chromatosome and stabilize condensed chromatin (PMID:16462749). The N-terminal domain sets overall chromatin-binding affinity while the C-terminal domain (CTD) dictates the nucleosomal interaction geometry; the CTD binds DNA major grooves with high affinity and releases linker DNA during nucleosome unwrapping and transcription-factor binding, with a short region at the start of the CTD controlling this behavior (PMID:22334665, PMID:12119037, PMID:35377618). H1-0 is loaded onto nucleosomes by chaperones including prothymosin-α, and is selectively removed from somatic nuclei by nucleoplasmin (PMID:30430826, PMID:8918467). Cell cycle-dependent CTD phosphorylation accumulates from interphase to mitosis and reduces nucleosome-bound CTD condensation, providing a switch that relaxes H1-0–imposed compaction (PMID:7191324, PMID:35618225). Genomically, H1-0 is enriched at nucleolus-associated repeats and represses megabase gene domains; this repressive activity restrains cancer cell self-renewal—and can be pharmacologically restored by HDAC inhibition—and is conversely required for myofibroblast activation and cardiac fibrosis through locus-specific control of H3K27 acetylation (PMID:25645921, PMID:27708074, PMID:32286289, PMID:38765203). H1-0 is regulated transcriptionally by histone acetylation, NF-κB, and oncogenic fusion proteins, and post-transcriptionally by brain-specific 3'-UTR RNA-binding proteins (PMID:7925412, PMID:12149419, PMID:40177616, PMID:9712912). Despite these roles, H1-0 is individually dispensable for mouse development because other H1 variants compensate to maintain H1-to-nucleosome stoichiometry (PMID:7604008, PMID:11689686).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 1980 Medium

    Established that H1.0 is dynamically phosphorylated across the cell cycle, linking its modification state to chromosome condensation.

    Evidence Radiolabeling and SDS-PAGE in synchronized CHO cells with EM-based cell cycle staging

    PMID:7191324

    Open questions at the time
    • Did not identify the kinases or specific phosphosites
    • Correlative with condensation, not causal
  2. 1985 Medium

    Connected H1.0 occupancy to transcriptional state in vivo and showed it confers a distinct, more extended chromatin architecture than other H1 subtypes.

    Evidence Nucleosome fractionation/Southern blot of liver genes and biophysical analysis of reconstituted chromatin

    PMID:2579343 PMID:4084523

    Open questions at the time
    • Correlation of H1.0 with repressed genes does not establish causality
    • Mechanistic basis of altered compaction undefined
  3. 1986 Medium

    Showed that in nondividing cells H1.0 is deposited without phosphorylation, dissociating its synthesis from the cell-cycle phosphorylation program.

    Evidence Radiolabeling and electrophoresis in growth-arrested neuroblastoma cells

    PMID:3955009

    Open questions at the time
    • Did not address functional consequence of unphosphorylated deposition
  4. 1995 High

    Determined whether H1.0 is essential, revealing functional redundancy among H1 variants that buffer its loss.

    Evidence Germline H1.0 knockout mice with chromatin H1/nucleosome stoichiometry analysis

    PMID:7604008

    Open questions at the time
    • Redundancy masks H1.0-specific roles
    • No tissue- or context-specific phenotype probed
  5. 1996 Medium

    Identified the chaperone-driven mechanism of linker-histone removal from somatic nuclei, ruling out phosphorylation as the trigger.

    Evidence Xenopus egg extract reconstitution and chromatin remodeling assays

    PMID:8918467

    Open questions at the time
    • In vitro extract system may not reflect somatic dynamics
    • Nucleoplasmin selectivity mechanism not resolved
  6. 2001 High

    Generalized the redundancy model by showing any single H1 subtype is dispensable provided total H1 stoichiometry is maintained.

    Evidence Single and double H1 knockout mouse lines with chromatin stoichiometry analysis

    PMID:11689686

    Open questions at the time
    • Does not reveal non-redundant H1.0 functions
    • Total H1 depletion phenotype not addressed here
  7. 2002 Medium

    Defined upstream regulators of H1.0 and a cellular requirement in dendritic cell differentiation, and characterized its high-affinity DNA binding chemistry.

    Evidence H1.0 knockout mice with hematopoietic differentiation assays plus NF-κB inhibition; DNA thermal denaturation/major-groove modification binding assays

    PMID:12119037 PMID:12149419

    Open questions at the time
    • NF-κB regulation shown by inhibition, not direct promoter occupancy
    • DC defect mechanism downstream of H1.0 unclear
  8. 2006 High

    Resolved the nucleosome-binding architecture of the globular domain as two cooperative DNA-binding sites forming the chromatosome.

    Evidence Systematic mutagenesis with in vivo FRAP and structural modeling in native chromatin

    PMID:16462749

    Open questions at the time
    • Atomic-resolution structure of the bound complex not provided
    • Did not address subtype-specific binding
  9. 2012 High

    Partitioned the domain logic of H1.0 binding—N-terminal domain controlling affinity, C-terminal domain controlling interaction geometry.

    Evidence Domain-swap and point mutagenesis with dual-color FRAP in living cells

    PMID:20444700 PMID:22334665

    Open questions at the time
    • Geometry differences inferred from binding dynamics, not direct structure
  10. 2013 High

    Mapped the H1.0 protein interactome to a nucleolar network including FACT and splicing factors, and quantified CTD-dominated DNA binding.

    Evidence Domain-resolved pull-down/LC-MS/MS with recombinant binding validation; ITC/CD DNA-binding measurements

    PMID:23435226 PMID:24036047

    Open questions at the time
    • Functional significance of FACT/splicing-factor interactions untested
    • Globular-domain DNA binding calorimetrically silent, limiting thermodynamic interpretation
  11. 2016 High

    Established H1.0 as a tumor-restraining factor that compacts megabase gene domains and enforces differentiation, and mapped its enrichment at nucleolus-associated repeats.

    Evidence Knockdown/re-expression across cancer types with genome-wide transcriptional analysis; ChIP-seq with cell fractionation

    PMID:25645921 PMID:27708074

    Open questions at the time
    • How H1.0 selects specific megabase domains is unresolved
    • Link between nucleolar enrichment and gene-domain repression not directly tied
  12. 2018 Medium

    Identified prothymosin-α as a chaperone that delivers H1.0 to nucleosomes, defining a deposition pathway.

    Evidence ITC binding measurement and in vitro nucleosome assembly with recombinant proteins

    PMID:30430826

    Open questions at the time
    • In vivo relevance of ProTα-mediated deposition not shown
    • Selectivity for H1.0 vs other variants untested
  13. 2022 High

    Mechanistically dissected the CTD-driven release of linker DNA during transcription-factor binding and quantified how CTD phosphorylation reduces nucleosome-bound condensation, while determining the unbound globular-domain structure.

    Evidence FRET-based in vitro nucleosome assays with synthetic PTM proteins; MS phosphosite mapping with phosphomimetic condensation assays; NMR structure with charge-variant thermostability

    PMID:35066947 PMID:35377618 PMID:35618225

    Open questions at the time
    • Phosphomimetics approximate but do not equal physiological phosphorylation
    • In vitro PTM effects differed from phosphomimetic studies, leaving the PTM role unsettled
    • Globular-domain structure determined unbound, not on nucleosome
  14. 2024 Medium

    Extended H1.0's role beyond repression to a required driver of myofibroblast activation and fibrosis, and revealed an autophagy-controlled degradation route governing chromatin accessibility.

    Evidence Fibroblast knockdown/overexpression with H3K27ac ChIP and in vivo cardiac fibrosis model; IFRD1 knockdown with co-IP and nucleophagy/chromatin accessibility assays

    PMID:38765203 PMID:38802351

    Open questions at the time
    • How a repressive linker histone activates pro-fibrotic transcription is mechanistically unclear
    • IFRD1-TRIM21-ATG14 nucleophagy pathway shown in single lab
  15. 2025 Medium

    Identified H1-0 as a transcriptionally induced mediator of an oncogenic fusion's repressive program in preleukemia.

    Evidence CRISPR-engineered hiPSC models, dual-luciferase promoter assays, and knockdown transcriptomics/single-cell sequencing

    PMID:40177616

    Open questions at the time
    • Direct ETV6::RUNX1 promoter occupancy vs activity not fully separated
    • Single-system model of preleukemia

Open questions

Synthesis pass · forward-looking unresolved questions
  • How H1.0 achieves locus selectivity—choosing specific megabase domains to repress versus the pro-activation it exerts in fibroblasts—and which kinases govern its cell-cycle CTD phosphorylation in vivo remain unresolved.
  • Targeting/recruitment mechanism to specific genomic domains unknown
  • Kinases responsible for cell-cycle CTD phosphorylation unidentified
  • Reconciliation of repressive vs activating roles in different cell types lacking

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003677 DNA binding 4 GO:0140110 transcription regulator activity 4 GO:0005198 structural molecule activity 2
Localization
GO:0005730 nucleolus 3 GO:0000228 nuclear chromosome 2 GO:0005634 nucleus 2
Pathway
R-HSA-74160 Gene expression (Transcription) 4 R-HSA-1266738 Developmental Biology 3 R-HSA-4839726 Chromatin organization 3
Partners
ARGCN5IFRD1PTMASF2/ASFU2AF65
Complex memberships
chromatosome

Evidence

Reading pass · 32 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2006 The globular domain of H1.0 binds to the nucleosome via two distinct DNA-binding sites formed by spatial clustering of multiple residues: one site interacts with the major groove near the nucleosome dyad, and the second site interacts with linker DNA adjacent to the nucleosome core. Multiple residues bind cooperatively to form a chromatosome structure that facilitates chromatin condensation. Systematic mutagenesis combined with in vivo FRAP (fluorescence recovery after photobleaching) and structural modeling in native chromatin Nature structural & molecular biology High 16462749
2012 The N-terminal domain of H1.0 determines overall chromatin binding affinity, while the C-terminal domain influences the nucleosomal interaction surface of the globular domain. Exchanging N-terminal domains between H1.0 and H1c swapped their binding affinities, while swapping C-terminal domains altered the chromatin interaction geometry. Domain swap and point mutagenesis combined with dual-color FRAP assay in living cells The Journal of biological chemistry High 22334665
2010 The nucleosome interaction surface of H1c globular domain is distinct from that of H1.0 globular domain, despite considerable structural conservation, suggesting the two subtypes bind the nucleosome with different orientations. Site-directed mutagenesis combined with in vivo photobleaching (FRAP) The Journal of biological chemistry Medium 20444700
2013 H1.0 interacts with an extensive network of proteins enriched in the nucleolus, including FACT and splicing factors SF2/ASF and U2AF65 (confirmed by direct binding), as well as rRNA biogenesis factors and ribosomal proteins. About one-third of H1.0-dependent interactions are mediated by the C-terminal domain, and two-thirds by the N-terminal domain/globular domain fragment. Protein pull-down with full-length H1.0 and CTD-deleted H1.0 from human nuclear extracts, LC-MS/MS proteomics, quantitative biophysical binding assays with recombinant proteins Nucleic acids research High 23435226
1996 H1.0 is selectively released from chromatin in Xenopus egg cytoplasm, and the molecular chaperone nucleoplasmin plays an important role in the selective removal of linker histones (including H1.0) from somatic nuclei. Phosphorylation of somatic linker histone variants does not direct their release from chromatin, and direct competition with cytoplasmic B4 histone does not determine their release. Biochemical reconstitution using Xenopus egg extracts; chromatin remodeling assays; phosphorylation analysis The EMBO journal Medium 8918467
1995 Mice completely lacking H1.0 develop and reproduce normally with no anatomic or histological abnormalities. In H1.0-knockout chromatin, other H1 subtypes (especially H1c, H1d, H1e) compensate to maintain normal H1-to-nucleosome stoichiometry, indicating functional redundancy among H1 variants. Gene knockout by homologous recombination in mouse ES cells; chromatin analysis of H1 stoichiometry Proceedings of the National Academy of Sciences of the United States of America High 7604008
2001 Single knockouts of H1c, H1d, or H1e, and their double knockouts with H1.0, all develop normally with normal H1-to-nucleosome stoichiometry, confirming that any individual H1 subtype (including H1.0) is dispensable for mouse development provided total H1 stoichiometry is maintained. Homologous recombination knockouts; double-knockout breeding; chromatin H1/nucleosome stoichiometry analysis Molecular and cellular biology High 11689686
2016 Silencing of H1.0 promotes maintenance of self-renewing cancer cells by inducing derepression of megabase-sized gene domains harboring downstream effectors of oncogenic pathways, demonstrating that H1.0 restricts long-term proliferative potential of cancer cells and drives their differentiation. H1.0 knockdown and re-expression in multiple cancer types; single-cell analysis; genome-wide transcriptional analysis Science (New York, N.Y.) High 27708074
2020 Quisinostat (HDAC inhibitor) re-expresses H1.0 in cancer cells, and H1.0 mediates the anti-self-renewal effects of Quisinostat. H1.0 re-expression inhibits cancer cell self-renewal without affecting normal stem cells, and hinders expansion of cells surviving targeted therapy in mouse lung cancer models. H1.0 knockdown and pharmacological induction; mouse models of lung cancer; cancer cell self-renewal assays Nature communications High 32286289
2024 H1.0 depletion prevents cytokine-induced fibroblast contraction, proliferation, and migration via inhibition of a transcriptome comprising extracellular matrix, cytoskeletal, and contractile genes through a process involving locus-specific H3K27 acetylation. H1.0 expression is necessary and sufficient to induce myofibroblast activation, and transient depletion prevents fibrosis in cardiac muscle in vivo. H1.0 knockdown and overexpression in fibroblasts; ChIP for H3K27 acetylation; in vivo cardiac fibrosis model Nature cardiovascular research High 38765203
2002 H1.0 is required for normal dendritic cell (DC) differentiation; H1.0-deficient mice show significantly decreased DC production while macrophage, granulocyte, and lymphocyte generation are normal. Transcription factor NF-κB is involved in regulation of H1.0 expression, and tumor-derived factors reduce H1.0 expression in hematopoietic progenitor cells to inhibit DC differentiation. H1.0 knockout mice; hematopoietic cell differentiation assays; NF-κB inhibition experiments Journal of leukocyte biology Medium 12149419
1985 H1.0-containing nucleosomes are preferentially associated with the alpha-fetoprotein gene (which is repressed in adult liver) but not with the expressed albumin gene, demonstrating selective association of H1.0 with transcriptionally repressed chromatin during liver development. Nucleosome fractionation from adult mouse liver chromatin; Southern blot analysis for specific gene association Nature Medium 2579343
2002 H1.0 and its C-terminal domain bind to the major groove of DNA with high affinity (~10^8 M^-1, covering ~10 bp per molecule). The globular domain alone binds much more weakly (~6×10^4 M^-1, covering ~3 bp) and shows no major groove interaction. Glucosylation projecting into the major groove of T4 DNA reduces the number of H1.0 binding sites, confirming major groove interaction. Thermal denaturation of DNA titrated with H1.0, full-length and domain fragments; comparison with wild-type and major-groove-modified T4 bacteriophage DNA Biochemistry Medium 12119037
1999 H1.0 specifically recognizes the central domain of four-way junction DNA via its globular domain, and the C-terminal domain makes additional contacts with regions distant from the crossover, as demonstrated by UV laser footprinting of specific guanine residues. UV laser footprinting of H1.0 and C-terminal deletion mutant with synthetic four-way junction DNA; immunofractionation Biochemistry Medium 10471283
1998 H1.0 contains multiple sequence elements that can function as nuclear localization signals (NLS). Transport of H1.0 into the nucleus is energy- and temperature-dependent and is competed by the SV40 T-antigen NLS, indicating use of an importin-dependent pathway. Digitonin-permeabilized cell import assay; transfection of H1.0-beta-galactosidase fusion constructs; competition with SV40 NLS peptide Experimental cell research Medium 9770363
1980 H1.0 (as BEP) undergoes cell cycle-dependent phosphorylation: little phosphorylation in G1-arrested cells, 1-2 sites phosphorylated in late interphase, and ~4 sites phosphorylated during mitosis. Mitotic phosphorylation is temporally correlated with chromosomal condensation during prophase/metaphase/anaphase and is reversed during exit from mitosis. Radiolabeling, SDS-PAGE electrophoresis, synchronized CHO cell populations, cell cycle analysis by electron microscopy Biochemistry Medium 7191324
1986 In nondividing cells, H1.0 is synthesized and deposited onto chromatin without accompanying phosphorylation, despite other H1 subtypes being phosphorylated upon synthesis. This demonstrates that phosphorylation of H1.0 is uncoupled from its synthesis when cells are arrested from dividing. Radiolabeling and electrophoretic analysis of H1 subfractions in mouse neuroblastoma cells blocked by butyrate, DMSO, or serum withdrawal Biochemistry Medium 3955009
1994 H1.0 gene expression is correlated with histone acetylation status. Trichostatin A (TSA), a specific histone deacetylase inhibitor, efficiently induces H1.0 gene expression. This induction is promoter-dependent (demonstrated by transfection of the H1.0 promoter) and is specific to H1.0, not shared by cell-cycle-dependent H1 or H4 genes. TSA treatment; TSA-resistant cell line comparison; transfection of H1.0 promoter-reporter constructs; cell cycle analysis European journal of biochemistry Medium 7925412
1993 The H1.0 promoter contains an 80 bp element (located ~430 bp upstream of the TATA box) necessary and sufficient for basal transcription, to which at least two nuclear factors of MW 90,000 and 30,000 bind; this binding is required for transcription. The basal element requires additional proximal promoter sequences for full activity. Promoter deletion analysis; in vitro footprinting; DMS interference; site-directed mutagenesis; UV-cross-linking; transfection reporter assays Nucleic acids research Medium 8451192
2018 H1.0 forms a 1:1 complex with its chaperone prothymosin-α (ProTα) with a KD of ~4.6×10^-7 M (measured by ITC). ProTα facilitates formation of the H1.0-nucleosome complex in vitro, suggesting a chaperone function in delivering H1.0 to nucleosomes rather than displacing it from chromatin. Isothermal titration calorimetry (ITC); in vitro nucleosome assembly assays with recombinant proteins Biochemistry Medium 30430826
2022 The H1.0 C-terminal domain (CTD) releases linker DNA during nucleosome partial unwrapping and transcription factor (TF) binding, while the globular domain remains bound to the nucleosome dyad. A 16 amino acid region at the beginning of the CTD is largely responsible for regulating nucleosome wrapping and TF binding within nucleosomes. Phosphorylation and citrullination PTMs have no detectable influence on nucleosome binding and wrapping and only minor impact on TF occupancy. In vitro fluorescence assays with fluorophores positioned throughout H1 and nucleosome; mutational studies; fully synthetic H1 with PTMs via native chemical ligation Biochemistry High 35377618
2022 Six phosphorylation sites were identified within the CTD of Xenopus H1.0. Phosphomimetic substitutions at S117E, S155E, S181E, S188E, and S192E significantly reduce nucleosome-bound H1.0 CTD condensation compared to unphosphorylated H1.0, and distinct phosphomimetics have unique effects on H1-dependent linker DNA trajectory. Mass spectrometry identification of phosphorylation sites; phosphomimetic mutagenesis; nucleosome-dependent CTD condensation assays; linker DNA trajectory analysis Molecular & cellular proteomics : MCP High 35618225
1981 The globular domain of H1.0 has a conformation and stability similar to that of the globular domain of H5, rather than to other H1 subtypes, as determined by NMR and optical spectroscopy. The globular regions of H1.0 and H5 are proposed to bind to the same specific site on the nucleosome. High-resolution NMR and optical spectroscopy (CD) of purified proteins European journal of biochemistry Medium 7318833
2022 The solution structure of the unbound globular domain (GD) of human H1.0 was determined by NMR. The structure is almost completely unperturbed by complex formation (except a loop between two antiparallel β-strands). Modulating the number of positive charges on the GD affects stability (26 K difference in melting temperature between net charge +5 and +13 variants) but not structure, suggesting positive charges have evolved for DNA-binding function rather than structural stability. NMR structure determination; thermostability measurements of 11 charge variants Protein science Medium 35066947
1998 Rat brain contains specific RNA-binding proteins (p40, p110, p70) that bind to a conserved portion of the H1.0 mRNA 3'-untranslated region and are expressed predominantly or exclusively in adult rat brain. These factors are proposed to regulate H1.0 mRNA stability and/or translation in neurons. RNase T1 protection assays with rat brain extracts; UV cross-linking; identification of specific 3'-UTR binding region The Journal of biological chemistry Medium 9712912
2013 H1.0 binds to calf thymus DNA with high affinity (Ka ~10^7 M^-1) primarily through its C-terminal domain; the electrostatic contribution to binding is small (6-17% of total ΔG). Binding H1.0-globular domain to DNA at 25°C is calorimetrically silent (no detectable ITC signal). Isothermal titration calorimetry (ITC) and circular dichroism with full-length H1.0 and isolated C-terminal and globular domains Biophysical chemistry Medium 24036047
2024 IFRD1 inhibits autophagy (via promoting proteasomal degradation of ATG14 in a TRIM21-dependent manner), protecting H1.0 from nucleophagic degradation under glutamine starvation. Depletion of IFRD1 increases autophagy flux leading to nucleophagic degradation of H1.0, resulting in globally enhanced chromatin accessibility, unchecked increases in ribosome and protein biosynthesis, and cancer cell exhaustive death. IFRD1 knockdown; autophagy flux measurement; co-IP for IFRD1-ATG14-TRIM21 interactions; nucleophagy assays; chromatin accessibility analysis Cell discovery Medium 38802351
2022 H1.0 induces expression of GCN5 and recruits GCN5 and androgen receptor (AR) to drive transcription of paclitaxel-resistance genes ABCB1 and ABCG2 in ovarian cancer cells. H1.0 levels are regulated by the PI3K/AKT pathway. Knockdown of H1.0 downregulates AR and sensitizes paclitaxel-resistant cells to paclitaxel. H1.0 knockdown and overexpression; PI3K inhibitor treatment; chromatin immunoprecipitation; gene expression analysis in paclitaxel-resistant cell lines Cancer science Medium 35639349
2025 H1-0 is upregulated by the ETV6::RUNX1 fusion protein via direct induction of H1-0 promoter activity (shown by dual-luciferase assays). H1-0 depletion specifically inhibits ETV6::RUNX1 signature genes including RAG1 and EPOR, identifying H1-0 as a key mediator of the repressive ETV6::RUNX1 transcriptional landscape in preleukemia. CRISPR/Cas9-engineered hiPSC models; dual-luciferase promoter assays; H1-0 knockdown with transcriptome analysis; single-cell sequencing HemaSphere Medium 40177616
1985 H1.0 induces a less efficient compaction of stripped chromatin than H1-1, resulting in a more extended chromatin structure as judged by orientational relaxation time measurements. H1.0 reconstituted chromatin shows reduced protection of DNA (longer free linker DNA) compared to H1-1, suggesting H1.0 confers a different chromatin structure with greater flexibility. Thermal denaturation, circular dichroism, electric birefringence, nuclease digestion of stripped/reconstituted rat liver chromatin Biochemistry Medium 4084523
1993 Immunoelectron microscopy demonstrates that H1.0 accumulates in condensed chromatin areas including perinucleolar chromatin, and is also found in perichromatin regions (sites of pre-mRNA synthesis), indicating H1.0 is not fully excluded from active chromatin. Immunofluorescence light microscopy and immunoelectron microscopy with monoclonal antibodies specific for H1.0 in MEL cells Experimental cell research Medium 8453989
2016 H1.0 is enriched at nucleolus-associated DNA repeats and chromatin domains (by ChIP-seq), while H1X is associated with coding regions and RNA polymerase II-enriched regions. This differential genomic distribution was established by ChIP-seq combined with cell fractionation. ChIP-sequencing and cell fractionation in human breast cancer cells The Journal of biological chemistry Medium 25645921

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 Individual somatic H1 subtypes are dispensable for mouse development even in mice lacking the H1(0) replacement subtype. Molecular and cellular biology 154 11689686
2006 Mapping the interaction surface of linker histone H1(0) with the nucleosome of native chromatin in vivo. Nature structural & molecular biology 151 16462749
1995 Mice develop normally without the H1(0) linker histone. Proceedings of the National Academy of Sciences of the United States of America 149 7604008
2016 The linker histone H1.0 generates epigenetic and functional intratumor heterogeneity. Science (New York, N.Y.) 144 27708074
1996 Remodeling somatic nuclei in Xenopus laevis egg extracts: molecular mechanisms for the selective release of histones H1 and H1(0) from chromatin and the acquisition of transcriptional competence. The EMBO journal 108 8918467
1988 Early increase in histone H1(0) mRNA during differentiation of F9 cells to parietal endoderm. The EMBO journal 100 2846273
1985 Association of histone H1(0) with a gene repressed during liver development. Nature 95 2579343
1993 All known human H1 histone genes except the H1(0) gene are clustered on chromosome 6. Genomics 75 8325638
2013 Linker histone H1.0 interacts with an extensive network of proteins found in the nucleolus. Nucleic acids research 65 23435226
1993 Developmental regulation and butyrate-inducible transcription of the Xenopus histone H1(0) promoter. Gene 60 8514185
2015 Genome distribution of replication-independent histone H1 variants shows H1.0 associated with nucleolar domains and H1X associated with RNA polymerase II-enriched regions. The Journal of biological chemistry 58 25645921
1981 Conformation studies of histone H1(0) in comparison with histones H1 and H5. European journal of biochemistry 58 7318833
1980 Amino acid analysis and cell cycle dependent phosphorylation of an H1-like, butyrate-enhanced protein (BEP; H1(0); IP25) from Chinese hamster cells. Biochemistry 57 7191324
1986 Butyrate induced accumulation of a 2.3 kb polyadenylated H1(0) histone mRNA in HeLa cells. Nucleic acids research 56 3020508
1991 Regulation of histone H1(0) accumulation during induced differentiation of murine erythroleukemia cells. Journal of molecular biology 53 1988682
1985 Effects of butyric acid on cell cycle regulation and induction of histone H1(0) in mouse cells and tissue culture. Inducibility of H1 (0)in the late S-G2 phase of the cell cycle. Journal of molecular biology 52 4009723
1981 Accumulation of histone H1(0) during chemically induced differentiation of murine neuroblastoma cells. European journal of biochemistry 50 6263627
2012 N- and C-terminal domains determine differential nucleosomal binding geometry and affinity of linker histone isotypes H1(0) and H1c. The Journal of biological chemistry 44 22334665
2002 H1(0) histone and differentiation of dendritic cells. A molecular target for tumor-derived factors. Journal of leukocyte biology 40 12149419
1984 Differential kinetics of histone H1(0) accumulation in neuronal and glial cells from rat cerebral cortex during postnatal development. Biochemical and biophysical research communications 40 6487308
1994 Relationship between core histone acetylation and histone H1(0) gene activity. European journal of biochemistry 38 7925412
1982 Antibodies against the folding domain of histone H5 cross-react with H1(0) but not with H1. The Journal of biological chemistry 38 6179941
1985 Differences in the condensation of chromatin by individual subfractions of histone H1: implications for the role of H1(0) in the structural organization of chromatin. Biochemistry 33 4084523
2020 Selective inhibition of cancer cell self-renewal through a Quisinostat-histone H1.0 axis. Nature communications 32 32286289
1994 H1(0) and H3.3B mRNA levels in developing rat brain. Neurochemical research 32 7877725
1989 Cloning and characterization of the mouse histone H1(0) promoter region. Gene 31 2806918
1985 The involvement of histone H1[0] in chromatin structure. Nucleic acids research 31 4000966
2010 Nucleosome interaction surface of linker histone H1c is distinct from that of H1(0). The Journal of biological chemistry 30 20444700
1991 Cell cycle-related accumulation of H1(0) mRNA: induction in murine erythroleukemia cells. Experimental cell research 29 2026174
1998 The histone H1(0) contains multiple sequence elements for nuclear targeting. Experimental cell research 28 9770363
2018 H1.0 Linker Histone as an Epigenetic Regulator of Cell Proliferation and Differentiation. Genes 27 29925815
2018 Binding Affinity and Function of the Extremely Disordered Protein Complex Containing Human Linker Histone H1.0 and Its Chaperone ProTα. Biochemistry 26 30430826
1994 Two mRNA species encoding the differentiation-associated histone H1(0) are produced by alternative polyadenylation in mouse. European journal of biochemistry 25 8168529
1986 Identification of histone H1(0) in Physarum polycephalum. Its high level in the plasmodial stage increases in amount and phosphorylation in the sclerotial stage. The Journal of biological chemistry 25 3944138
2024 Histone H1.0 couples cellular mechanical behaviors to chromatin structure. Nature cardiovascular research 24 38765203
2005 Immunohistochemical demonstration of histone H1(0) in human breast carcinoma. Histochemistry and cell biology 23 16158288
1987 Relationship of H1(0) histone to differentiation and cancer. Cancer biochemistry biophysics 23 3325165
2016 Extracellular vesicles shed by melanoma cells contain a modified form of H1.0 linker histone and H1.0 mRNA-binding proteins. International journal of oncology 21 27633859
1993 Basal level transcription of the histone H1(0) gene is mediated by a 80 bp promoter fragment. Nucleic acids research 21 8451192
1987 The histone H1(0)/H5 variant and terminal differentiation of cells during development of Xenopus laevis. Differentiation; research in biological diversity 20 3327718
1993 Light and electron microscope immunocytochemical analyses of histone H1(0) distribution in the nucleus of Friend erythroleukemia cells. Experimental cell research 19 8453989
1983 Extent of histone modifications and H1(0) content during cell cycle progression in the presence of butyrate. Experimental cell research 19 6617774
2024 IFRD1 promotes tumor cells "low-cost" survival under glutamine starvation via inhibiting histone H1.0 nucleophagy. Cell discovery 18 38802351
2007 The differentiation-associated linker histone, H1.0, during the in vitro aging and senescence of human diploid fibroblasts. Annals of the New York Academy of Sciences 18 17460199
1998 H1(0) RNA-binding proteins specifically expressed in the rat brain. The Journal of biological chemistry 18 9712912
1995 Accumulation of histone H1(0) during early Xenopus laevis development. Experimental cell research 17 7796895
1990 The expression of the histone H1 (0) gene in the human hepatoma cell line HepG2 is independent of the state of cell proliferation. Differentiation; research in biological diversity 17 2167251
1985 Induction of histone H1(0) differs with different treatments among different cell lines. FEBS letters 17 4040875
1993 Differential expression and gonadal hormone regulation of histone H1(0) in the developing and adult rat brain. Brain research. Developmental brain research 16 8513555
1985 Immunohistochemical distribution of the histone H1(0)/H5 variant in various tissues of adult Xenopus laevis. Cell differentiation 15 3891099
2022 H1.0 C Terminal Domain Is Integral for Altering Transcription Factor Binding within Nucleosomes. Biochemistry 14 35377618
1986 H1(0) histones of normal and cancer human cells. Amino acid composition of H1 purified by polyacrylamide gel electrophoresis. Molecular and cellular biochemistry 14 3022119
1985 Histone H1(0) is distributed unlike H1 in chromatin aggregation. FEBS letters 14 3979562
2020 Effect of Phosphorylation on the Structural Behaviour of Peptides Derived from the Intrinsically Disordered C-Terminal Domain of Histone H1.0. Chemistry (Weinheim an der Bergstrasse, Germany) 13 32073728
2016 Regulation of Cellular Dynamics and Chromosomal Binding Site Preference of Linker Histones H1.0 and H1.X. Molecular and cellular biology 13 27528617
2002 Histone h1(0) and its carboxyl-terminal domain bind in the major groove of DNA. Biochemistry 13 12119037
1996 Chromosome mapping of rat histone genes H1fv, H1d, H1t, Th2a and Th2b. Cytogenetics and cell genetics 13 9040779
1992 Multiple cis-acting elements of the proximal promoter region are required for basal level transcription of the H1(0) histone gene. Biochemical and biophysical research communications 13 1445349
1986 Immunological evidence for an H1(0) type of histone protein in chicken liver. Differentiation; research in biological diversity 13 3536645
2003 Construction of an EGF receptor-mediated histone H1(0)-based gene delivery system. Journal of cancer research and clinical oncology 12 12884022
1992 Increased histone H1(0) expression in differentiating mouse erythroleukemia cells is related to decreased cell proliferation. Experimental cell research 12 1639146
1990 Interaction of histones H1 and H1(0) with superhelical and linear DNA. FEBS letters 12 2335225
1987 Histone H1(0) is synthesized by human lymphocytic leukemia cells but not by normal lymphocytes. Blood 12 3651604
1983 Dynamics of H1(0) content in rat liver after partial hepatectomy. The International journal of biochemistry 12 6653867
1983 Histone H1(0) expression during developmental growth of rat liver. Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire 12 6667425
1999 Sequence preference of mouse H1(0) and H1t. Biochemistry 11 10529182
1995 Developmentally regulated chromatin acetylation and histone H1(0) accumulation. The International journal of developmental biology 11 8619958
1993 Specific expression in adult mice and post-implantation embryos of a transgene carrying the histone H1(0) regulatory region. Differentiation; research in biological diversity 11 8299878
2016 Nuclear and nucleolar activity of linker histone variant H1.0. Cellular & molecular biology letters 10 28536618
1999 Ultraviolet laser footprinting of histone H1(0)-four-way junction DNA complexes. Biochemistry 10 10471283
1990 Accessibility of histone H1(0) and its structural domains to antibody binding in extended and folded chromatin. Molecular and cellular biochemistry 10 1694955
1987 Autoantibodies against the H1(0) subtype of histone H1. Clinical immunology and immunopathology 10 2824111
2022 H1.0 induces paclitaxel-resistance genes expression in ovarian cancer cells by recruiting GCN5 and androgen receptor. Cancer science 9 35639349
2002 The effect of the histone deacetylase inhibitor, trichostatin A, on total histone synthesis, H1(0) synthesis and histone H4 acetylation in peripheral blood lymphocytes increases as a function of increasing age: a model study. Experimental gerontology 9 11772521
1995 Variation of H1(0) content throughout the cell cycle in regenerating rat liver. Experimental cell research 9 7698253
2022 Structure, dynamics, and stability of the globular domain of human linker histone H1.0 and the role of positive charges. Protein science : a publication of the Protein Society 8 35066947
2018 A CON-based NMR assignment strategy for pro-rich intrinsically disordered proteins with low signal dispersion: the C-terminal domain of histone H1.0 as a case study. Journal of biomolecular NMR 8 30414042
1988 Proliferation and differentiation are not directly related to H1(0) accumulation in cultured glial cells. Brain research 8 3219583
1985 Histone H1(0): a maintainer of the differentiated cell state? The International journal of biochemistry 8 2408935
2022 Analysis of histone variant constraint and tissue expression suggests five potential novel human disease genes: H2AFY2, H2AFZ, H2AFY, H2AFV, H1F0. Human genetics 7 35072799
1990 Increased level of histone H1(0) messenger RNA in hypoxic Ehrlich ascites tumor cells. Experimental cell research 7 2328774
1989 Expression of mouse histone H1(0) promoter sequences following microinjection into Xenopus oocytes and developing embryos. The International journal of developmental biology 6 2518451
2013 Calorimetric studies of the interactions of linker histone H1(0) and its carboxyl (H1(0)-C) and globular (H1(0)-G) domains with calf-thymus DNA. Biophysical chemistry 5 24036047
1997 Histone H1(0) expression is restricted to progenitor cells during human hematopoiesis. European journal of cell biology 5 9013724
1995 Cloning and analysis of the coding region of the histone H1(0)-encoding gene from rat PC12 cells. Gene 5 8543182
1991 Antigenic structure of histone H1(0). Molecular and cellular biochemistry 5 1724285
1988 Histones H1(0) and H5 share common epitopes with RNA polymerase II. The Journal of biological chemistry 5 2454917
1992 Histone H1(0) mRNA and protein accumulate early during retinoic acid induced differentiation of synchronized embryonal carcinoma cells. Molecular biology reports 4 1545783
2025 H1-0 is a specific mediator of the repressive ETV6::RUNX1 transcriptional landscape in preleukemia and B cell acute lymphoblastic leukemia. HemaSphere 3 40177616
2022 Identification and Analysis of Six Phosphorylation Sites Within the Xenopus laevis Linker Histone H1.0 C-Terminal Domain Indicate Distinct Effects on Nucleosome Structure. Molecular & cellular proteomics : MCP 3 35618225
2017 Preparative two-step purification of recombinant H1.0 linker histone and its domains. PloS one 3 29206861
2014 Cloning of cDNAs for H1F0, TOP1, CLTA and CDK1 and the effects of cryopreservation on the expression of their mRNA transcripts in yak (Bos grunniens) oocytes. Cryobiology 3 24854867
1996 Uncoordinated regulation of histone H1(0)synthesis and H1(0) mRNA level. Biochemistry and molecular biology international 3 9132149
1993 Altered levels of histone H1(0) and DNA topoisomerase activity in the liver of the tumour-bearing rat. Biochemistry and molecular biology international 3 8220251
1993 Effect of alpha 1-adrenergic blockade on nucleolar growth, chromatin relaxation, and histone H1(0) content in regenerating liver. Experimental cell research 3 8416799
1989 Changes in the synthesis of histone H1(0) and H1 in rat FRTL-5 thyroid cells exposed to thyrotropin. Life sciences 3 2601573
1988 Conformational effects of histones H1 on DNA structure. Comparative study between H1-1, H1(0), H5 and sperm holothuria phi 0. Biophysical chemistry 3 3233301
1984 On the occurrence of polymers of H1, H1(0) and H5 in extracts of whole tissues. Artificial production during protein preparation. Biochimica et biophysica acta 3 6498205
1991 Possible role of histone acetylation and histone H1(0) replacement for the initiation of replication in regenerating rat liver. The Biochemical journal 2 1764040
1986 In nondividing cells, histone H1(0) is synthesized and deposited onto chromatin without accompanying phosphorylation. Biochemistry 2 3955009

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