Affinage

GATA3

Trans-acting T-cell-specific transcription factor GATA-3 · UniProt P23771

Length
443 aa
Mass
47.9 kDa
Annotated
2026-06-10
100 papers in source corpus 32 papers cited in narrative 32 extracted findings
Cross-family judge vs UniProt: tie faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

GATA3 is a dual zinc-finger DNA-binding transcription factor that acts as a lineage-determining master regulator across immune, epithelial, neural, and developmental programs by binding GATA motifs and directing context-specific transcriptional outputs (PMID:10935639, PMID:17658278, PMID:31634421). In type 2 immunity it drives the Th2/ILC2 effector program, where its expression is controlled by Notch signaling and by lineage-restricted enhancers and super-enhancers that distinguish innate from adaptive type 2 cells (PMID:17658278, PMID:34353913, PMID:38969652, PMID:38923989), and its phosphorylated form binds the IL-5 and IL-13 promoters to drive cytokine production (PMID:23851685). GATA3 transcriptional activity is shaped by physical partners that tune target gene programs: it complexes with Ruvbl2 to repress the CDK inhibitor Cdkn2c and promote Th2 proliferation (PMID:24167278), with Bcl11b to limit Th2 cytokine output (PMID:29514917), and recruits the MLL4/UTX chromatin-remodeling machinery (UTX, ASH2L, RBBP5) to control a GATA3-UTX-Dicer axis that suppresses EMT (PMID:31685800). Its abundance and chromatin engagement are gated post-translationally: p38 MAP kinase phosphorylation promotes nuclear import via importin-alpha (PMID:18691059), CBP/p300-mediated acetylation (at K119, reversed by HDAC1/2/3) attenuates DNA binding and suppresses invasion (PMID:29453984, PMID:36329027), and protein stability is set by ubiquitin/proteasome turnover, with Gfi1 stabilizing GATA3 and Sox12-directed Fbw7 promoting its degradation (PMID:18701459, PMID:32152552). In epithelial cancers GATA3 functions broadly as a suppressor of metastasis and EMT—inducing miR-29b to silence pro-metastatic targets (PMID:23354167), enforcing luminal over basal mammary differentiation downstream of BRCA1 (PMID:34373738, PMID:34976209)—while in other contexts it stabilizes HIF-1alpha to enhance invasiveness (PMID:28263977) and confers therapeutic resistance by repressing CYB5R2 to block ferroptosis (PMID:37230023). Haploinsufficiency of GATA3 causes HDR syndrome (hypoparathyroidism, sensorineural deafness, renal anomaly) through loss of DNA binding (PMID:10935639).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 2000 High

    Established GATA3 as a haploinsufficient disease gene and linked its developmental role mechanistically to its DNA-binding function.

    Evidence Deletion mapping and mutation analysis in HDR patients with DNA-binding assays of mutant GATA3

    PMID:10935639

    Open questions at the time
    • Did not identify the direct parathyroid/auditory/renal target genes
    • No structural basis for loss-of-function mutations defined
  2. 2004 Medium

    Placed GATA3 upstream of inner-ear differentiation programs, showing it is required for NeuroD and Gata2 expression and ear morphogenesis.

    Evidence Antisense knockdown in auditory neuroblast line and Gata3-knockout mouse expression analysis

    PMID:15003631 PMID:15499560

    Open questions at the time
    • Direct vs. indirect regulation of NeuroD/Gata2 not distinguished
    • No ChIP evidence of direct promoter binding
  3. 2007 High

    Resolved how the Th2 program activates GATA3 by showing Notch directly induces a developmentally regulated Gata3 transcript.

    Evidence Activated Notch1 allele in CD4+ T cells with transcript-specific analysis and genetic epistasis

    PMID:17658278

    Open questions at the time
    • Mechanism of Notch synergy with GATA3 at IL-4 not fully mapped
    • Other upstream inducers not addressed
  4. 2008 Medium

    Defined two layers of GATA3 regulation: kinase-driven nuclear import and proteasomal control of protein stability.

    Evidence Subcellular localization and kinase inhibitor studies (review summary); Gfi1 knockout with proteasome analysis and GATA3 rescue

    PMID:18691059 PMID:18701459

    Open questions at the time
    • p38/importin-alpha mechanism summarized in a review rather than primary single study
    • E3 ligase mediating degradation not identified at this stage
  5. 2013 High

    Connected GATA3 to phosphorylation-dependent chromatin engagement in ILC2s and to metastasis suppression via a microRNA program.

    Evidence p38 inhibition with ChIP of phospho-GATA3 at IL-5/IL-13 promoters and conditional KO; GATA3/miR-29b overexpression-knockdown in cancer cells and mouse models

    PMID:23354167 PMID:23851685

    Open questions at the time
    • Phosphosite(s) on GATA3 not mapped
    • Direct vs. indirect induction of miR-29b not fully established
  6. 2014 High

    Demonstrated cell-intrinsic GATA3 requirements for innate lymphoid lineages and epithelial differentiation programs beyond T cells.

    Evidence Gata3-deficient fetal liver chimeras with infection challenge; siRNA/enforced expression in bladder cancer with EMT readouts; GATA3 suppression of adipogenesis via PPARgamma promoter binding

    PMID:24419270 PMID:24448324 PMID:26109067

    Open questions at the time
    • Direct ILC3 target genes not defined
    • Bladder cancer findings from a single lab
  7. 2016 High

    Identified GATA3 as a context-dependent repressor of another transcription factor, suppressing GCM1 activity to restrain trophoblast invasion.

    Evidence Co-IP with domain mapping, luciferase assays, and GATA3 knockdown in trophoblast lines; RNA-seq of trophoblast knockdown

    PMID:26899996 PMID:27733378

    Open questions at the time
    • Mechanism of GCM1 transcriptional repression by GATA3 not resolved
    • Trophoblast target genes not validated as direct
  8. 2017 High

    Revealed a pro-invasive GATA3 function via non-canonical stabilization of HIF-1alpha against proteasomal degradation.

    Evidence Reciprocal Co-IP, ubiquitination assay, ChIP of GATA3/HIF-1alpha at target promoters, HIF-1alpha knockdown rescue

    PMID:28263977

    Open questions at the time
    • Reconciliation with GATA3's metastasis-suppressive role left open
    • Structural basis of the GATA3-HIF-1alpha interaction unknown
  9. 2018 High

    Established acetylation as a DNA-binding switch and identified Bcl11b as a partner that recalibrates the Th2 cytokine program.

    Evidence Co-IP/mass-spec mapping of K119 acetylation with CBP writer and HDAC erasers and mimetic mutants; Bcl11b Co-IP, ChIP-seq co-occupancy and GATA3-dependence test

    PMID:29453984 PMID:29514917

    Open questions at the time
    • Stoichiometry and dynamics of K119 acetylation in vivo not measured
    • How Bcl11b alters GATA3 target selection mechanistically unclear
  10. 2019 High

    Defined GATA3 as a recruiter of chromatin-modifying machinery and a partner of additional transcription factors driving lineage and oncogenic programs.

    Evidence Co-IP of GATA3 with UTX/ASH2L/RBBP5 plus ChIP-seq/RNA-seq and in vivo assays; ISL1 Co-IP and co-occupancy in neuroblastoma; Gata3 ChIP at Runx1 enhancers in AGM stroma

    PMID:30867811 PMID:31634421 PMID:31685800

    Open questions at the time
    • Rules governing complex assembly (MLL4 vs. ISL1) by cell type unknown
    • Direct vs. cooperative binding at shared loci not dissected
  11. 2020 High

    Completed the GATA3 degradation circuit by naming Fbw7 as the E3 ligase directed by Sox12.

    Evidence Sox12 gain/loss, proteasome inhibition, GATA3 ubiquitination assay, Fbw7 knockdown rescue in Th2 cells

    PMID:32152552

    Open questions at the time
    • Degron and phosphodegron requirements on GATA3 not mapped
    • Interplay with Gfi1-mediated stabilization not addressed
  12. 2021 High

    Positioned GATA3 in the BRCA1 mammary tumor-suppressor axis and revealed roles in ferroptosis-linked drug resistance.

    Evidence BRCA1/GATA3 depletion, promoter methylation, mouse models and GATA3 reconstitution; ChIP/luciferase of CYB5R2 with ferroptosis assays and xenografts; enhancer deletion of Gata3 +674/762 in ILC2s

    PMID:34353913 PMID:34373738 PMID:37230023

    Open questions at the time
    • How GATA3 toggles between EMT suppression and resistance programs unresolved
    • Direct CYB5R2 binding-site architecture not detailed
  13. 2022 High

    Extended acetylation control to additional acetyltransferases and revealed therapeutic-vulnerability and differentiation roles in cancer.

    Evidence p300 acetylation/DNA-binding assays in T-cell neoplasms; GATA3/MDM2 synthetic lethality across cell, PDO and PDX models with p53/PI3K dissection; Gata3/p18 double-mutant mouse mammary tumor models

    PMID:34976209 PMID:35440675 PMID:36329027

    Open questions at the time
    • p300 study mechanistic detail limited to abstract-level
    • Mechanism linking GATA3 loss to MDM2 dependency not fully defined
  14. 2023 Medium

    Identified a GATA3-controlled vascular program in liver regeneration via PEDF/VEGFA.

    Evidence AAV-CRISPR screening in hepatectomy mice, patient-derived hepatic organoids, ALPPS model with PEDF-neutralizing antibody

    PMID:37918568

    Open questions at the time
    • Direct vs. indirect PEDF/VEGFA regulation not established
    • Single-lab finding
  15. 2024 High

    Showed lineage-specific cis-regulatory elements explain how the same GATA3 program is deployed differently across type 2 cells and stimuli.

    Evidence CRISPR deletion of G3SE super-enhancer with scRNA-seq; CRISPR deletion of mG900 distal enhancer with 4C-seq and allergic airway models

    PMID:38923989 PMID:38969652

    Open questions at the time
    • Trans-factors binding these enhancers not identified
    • Cnot6l identified as a 'possible' GATA3 target, not validated as direct

Open questions

Synthesis pass · forward-looking unresolved questions
  • How GATA3 selects between opposing transcriptional outcomes (metastasis suppression vs. HIF-1alpha-driven invasion; luminal differentiation vs. drug-resistance programs) in a given cellular context remains unresolved.
  • No unified model integrating PTM state, partner availability, and enhancer usage to predict target selection
  • Structural basis of partner-directed cofactor switching unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 6 GO:0003677 DNA binding 5
Localization
GO:0005634 nucleus 2 GO:0005829 cytosol 1
Pathway
R-HSA-168256 Immune System 7 R-HSA-74160 Gene expression (Transcription) 6 R-HSA-1266738 Developmental Biology 5 R-HSA-1643685 Disease 5 R-HSA-4839726 Chromatin organization 1
Partners
BCL11BCBP/CREBBPFBW7/FBXW7GCM1HIF1AISL1RUVBL2UTX/KDM6A
Complex memberships
MLL4/COMPASS-like (UTX, ASH2L, RBBP5)

Evidence

Reading pass · 32 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 GATA3 haploinsufficiency causes HDR syndrome (hypoparathyroidism, sensorineural deafness, renal anomaly). Deletion mapping defined a critical 200 kb region containing GATA3; nonsense mutations and intragenic deletions in HDR patients predicted loss of function, confirmed by absence of DNA binding by the mutant GATA3 protein in binding assays. Deletion mapping, mutation analysis, DNA-binding assay with mutant GATA3 protein Nature High 10935639
2013 GATA3 suppresses breast cancer metastasis and alters the tumor microenvironment by inducing microRNA-29b (miR-29b) expression; miR-29b targets pro-metastatic regulators including VEGFA, ANGPTL4, PDGF, LOX, MMP9, ITGA6, ITGB1, and TGFB. Overexpression/knockdown of GATA3 and miR-29b in breast cancer cells and mouse models; target gene identification by functional assays Nature Cell Biology High 23354167
2007 Notch signaling directly regulates Gata3 transcription during Th2 cell differentiation. Activated Notch1 specifically and directly upregulates a developmentally regulated Gata3 transcript containing exon 1a sequences; Notch acts in parallel with GATA-3 to synergistically activate IL-4 expression. Activated Notch1 allele introduction into CD4+ T cells; transcript-specific analysis; genetic epistasis Immunity High 17658278
2008 In human T lymphocytes, GATA3 is normally localized to the cytoplasm, but upon activation via TCR (CD3) and co-stimulatory receptor CD28, GATA3 is phosphorylated by p38 MAP kinase and translocates to the nucleus via the nuclear import protein importin-alpha. Corticosteroids bound to glucocorticoid receptors inhibit GATA3 function by competing for nuclear entry via importin-alpha and by inhibiting p38 MAP kinase through induction of MAP kinase phosphatase-1. Subcellular localization studies, kinase inhibitor assays, nuclear import pathway dissection Current Molecular Medicine Medium 18691059
2013 p38 MAP kinase activation in natural helper (NH/ILC2) cells leads to phosphorylation of GATA3; the phosphorylated form of GATA3 binds to the IL-5 and IL-13 promoters. p38 inhibition blocks this phosphorylation and blocks IL-5, IL-13, and IL-6 production. Mature NH cells lacking Gata3 are impaired in proliferation and production of IL-5 and IL-13. p38 inhibitor (SB203580) treatment, ChIP showing phospho-GATA3 binding to IL-5/IL-13 promoters, conditional Gata3 knockout in NH cells Journal of Immunology High 23851685
2008 Gfi1 (growth factor independent-1), a downstream target of the ERK MAPK cascade, stabilizes GATA3 protein in Th2 cells. In the absence of Gfi1, enhanced ubiquitin/proteasome-dependent degradation of GATA3 protein was observed; overexpression of GATA3 rescued Th2 cell functional defects in Gfi1-deficient cells. Gfi1 knockout T cells, ubiquitin-proteasome pathway analysis, GATA3 overexpression rescue experiments Journal of Biological Chemistry High 18701459
2013 Gata3 associates with RuvB-like protein 2 (Ruvbl2) and directly represses expression of the CDK inhibitor Cdkn2c at its locus in an Ruvbl2-dependent manner, thereby facilitating Th2 cell proliferation. Co-immunoprecipitation of Gata3-Ruvbl2 complex, ChIP showing Gata3 binding to Cdkn2c locus, Cdkn2c knockdown rescue of Gata3-deficient proliferation defect PNAS High 24167278
2017 GATA3 physically associates with HIF-1α under hypoxia to inhibit ubiquitination and proteasomal degradation of HIF-1α (independent of prolyl hydroxylation), thereby stabilizing HIF-1α. The GATA3/HIF-1α complex binds to and regulates HIF-1 target genes, enhancing cancer cell invasiveness. Co-IP of GATA3-HIF-1α, ubiquitination assay, ChIP showing GATA3/HIF-1α complex at target gene promoters, HIF-1α knockdown reversal of GATA3-mediated invasiveness Oncogene High 28263977
2018 Bcl11b binds to GATA3 through protein-protein interaction and colocalizes with GATA3 at cis-regulatory elements in Th2 cells. The GATA3/Bcl11b complex limits Th2 cytokine (IL-4, IL-5, IL-13) expression; upregulation of these cytokines in Bcl11b-deficient Th2 cells is completely GATA3-dependent. Co-IP of Bcl11b-GATA3, ChIP-seq co-occupancy analysis, Bcl11b knockout with GATA3 dependence test, RNA-seq Journal of Experimental Medicine High 29514917
2020 Sox12 promotes Fbw7-mediated ubiquitination and proteasomal degradation of GATA3 protein in Th2 cells without affecting GATA3 mRNA. Fbw7 knockdown partially abrogates Sox12-mediated GATA3 suppression, identifying Fbw7 as the E3 ligase responsible for GATA3 degradation. Sox12 overexpression/knockout, proteasome inhibitor assays, ubiquitination assay of GATA3, Fbw7 knockdown rescue Cellular & Molecular Immunology High 32152552
2019 GATA3 recruits the chromatin-remodeling MLL4 complex and directly interacts with UTX (histone H3K27 demethylase), ASH2L, and RBBP5. The GATA3/UTX complex synergistically regulates a cohort of genes including Dicer and UTX, controlling the EMT program; the GATA3-UTX-Dicer axis inhibits EMT, invasion, and metastasis. Co-IP of GATA3 with UTX/ASH2L/RBBP5, RNA-seq, ChIP-seq, in vitro and in vivo functional assays Cell Death & Disease High 31685800
2018 GATA3 interacts with and is acetylated by the acetyltransferase CBP at lysine 119 (K119). Class I deacetylases HDAC1, HDAC2, and HDAC3 deacetylate GATA3. Acetylation-mimicking K119Q mutant GATA3 inhibits lung adenocarcinoma cell migration and invasion with concomitant downregulation of EMT transcription factors Slug, Zeb1, and Zeb2. Co-IP of GATA3-CBP interaction, mass spectrometry identification of acetylation site, HDAC deacetylation assay, acetylation-mimetic mutant functional assays Biochemical and Biophysical Research Communications High 29453984
2021 BRCA1 depletion stimulates methylation of the GATA3 promoter, thereby repressing GATA3 transcription. GATA3 functions downstream of BRCA1 to suppress EMT in mammary tumor development; reconstitution of GATA3 in BRCA1-deficient tumor cells activates mesenchymal-epithelial transition and suppresses tumor initiation and metastasis. BRCA1/GATA3 depletion in human cells, promoter methylation analysis, genetically engineered mouse models, GATA3 reconstitution rescue experiments Theranostics High 34373738
2016 GATA3 interacts with GCM1 through the DNA-binding domain and first transcriptional activation domain of GCM1, and through the transcriptional activation domains and zinc finger 1 domain of GATA3. GATA3 does not affect GCM1 DNA binding but suppresses GCM1 transcriptional activity, thereby reducing HtrA4 expression and inhibiting trophoblast cell invasion. Co-IP of GATA3-GCM1, domain mapping by deletion mutants, luciferase transcriptional activity assay, GATA3 knockdown in trophoblast cell lines Scientific Reports High 26899996
2015 GATA3 suppresses adipogenesis by binding to the promoter of PPARγ and other key adipogenic regulators. AMPK activation by AICAR increases GATA3 expression through Wnt/β-catenin signaling: AMPK phosphorylates and inhibits GSK3β, stabilizing β-catenin, which then interacts with TCF to induce GATA3 transcription. AICAR also relieves CtBP co-repressor binding at the GATA3 promoter. ChIP showing GATA3 binding to PPARγ promoter, β-catenin/TCF reporter assay, AMPK/GSK3β phosphorylation assays, Wnt pathway inhibition Journal of Biological Chemistry High 26109067
2004 In auditory neuroblasts, GATA3 knockdown via antisense oligonucleotides reversibly downregulates NeuroD expression, demonstrating that NeuroD expression is dependent on GATA3 in auditory neuroblasts. GATA3 and NeuroD mark distinct neuronal populations: at E13.5 most auditory neurons express GATA3 but not NeuroD, whereas vestibular neurons express NeuroD but not GATA3. Antisense oligonucleotide knockdown in conditionally immortal auditory neuroblast cell line; immunostaining and expression analysis in vivo Mechanisms of Development Medium 15003631
2004 In Gata3-knockout mice, Gata2 expression is delayed and strongly reduced in the otic epithelium, indicating that Gata3 is required for normal Gata2 expression in the inner ear. Ear morphogenesis is blocked in Gata3-deficient embryos. Gata3 knockout mouse analysis, in situ hybridization and immunostaining for Gata2 expression in Gata3-/- embryos Developmental Dynamics Medium 15499560
2014 Cell-intrinsic Gata3 expression in fetal liver hematopoietic precursors is required for development of gut RORγt+ ILC3 subsets. Gata3-deficient chimeric mice lack all intestinal RORγt+ ILC3 subsets and show defective IL-22 production early after Citrobacter rodentium infection. Chimeric mice generated with Gata3-deficient fetal liver hematopoietic precursors; flow cytometry; infection challenge model Journal of Experimental Medicine High 24419270
2019 Gata3 directly targets Runx1 in stromal cells of the aorta-gonads-mesonephros (AGM) region, binding to specific enhancer elements at the Runx1 locus. This interaction promotes haematopoietic stem cell emergence in the embryo, with Runx1 expression reduced in Gata3-/- embryos. ChIP-seq and ChIP-on-chip in AGM-derived stromal cell line; co-localization by immunostaining; Gata3-/- embryo analysis IUBMB Life High 31634421
2017 GATA3 knockdown in keratinocytes significantly reduces filaggrin (FLG) mRNA expression under atopic dermatitis-like inflammatory conditions; overexpression of GATA3 upregulates both filaggrin and filaggrin-2 (FLG2) mRNA expression, demonstrating GATA3 regulates epidermal barrier genes. shRNA knockdown and GATA3 overexpression in primary human keratinocytes; RT-qPCR Scientific Reports Medium 28928464
2016 GATA3 knockdown in first trimester trophoblast cells (HTR8/SVneo) decreases cell migration and invasion. RNA-seq of GATA3 knockdown cells identified 96 differentially regulated genes including CTGF, CYR61, ADAMTS12, and TIMP3. Estradiol downregulates GATA3 expression in these cells. siRNA knockdown in trophoblast cell line, migration/invasion assays, RNA-seq, estradiol treatment Biology of Reproduction Medium 27733378
2021 GATA3 mediates doxorubicin resistance by transcriptionally repressing CYB5R2, thereby maintaining iron homeostasis and inhibiting ferroptosis. ChIP and luciferase assays confirmed direct GATA3 regulation of CYB5R2 expression. GATA3 overexpression/knockdown, ChIP assay, luciferase reporter assay, iron/ROS/lipid peroxidation detection, in vivo xenograft Drug Resistance Updates High 37230023
2022 p300-dependent acetylation regulates GATA3-mediated transcription in T-cell neoplasms by attenuating GATA3 DNA binding. Biochemical acetylation assays, DNA binding assays with acetylated GATA3 Blood Cancer Journal Medium 36329027
2021 GATA3 disruption in mouse embryos leads to craniofacial microsomia and syngnathia (bony fusion of jaws), resulting from changes in BMP4 and FGF8 gene regulatory networks within neural crest cells near the maxillomandibular junction, demonstrating GATA3 is essential for establishing network factors that separate upper and lower jaw patterning domains. Conditional Gata3 disruption in mouse embryos, in situ hybridization and immunostaining for BMP4 and FGF8 Development Medium 34383890
2022 Loss of GATA3 function in p18-deficient background in mice directly induces basal-like mammary tumors. Haploid loss of Gata3 impairs luminal differentiation, promotes basal differentiation, and p18 is identified as a downstream target of GATA3 that restrains mammary epithelial cell proliferation. Genetically engineered mouse models (Gata3/p18 double mutants), mammary gland phenotyping, immunostaining, flow cytometry Theranostics High 34976209
2019 ISL1 physically interacts with GATA3, and genome-wide mapping shows substantial co-occupancy of ISL1 and GATA3 binding regions in neuroblastoma cells; together they synergistically regulate oncogenic pathways including LMO1 and LIN28B. Co-IP of ISL1-GATA3, ISL1 ChIP-seq, RNA-seq, neuroblastoma cell KD functional assays Theranostics Medium 30867811
2021 A Gata3 enhancer (Gata3 +674/762) controls ILC2 differentiation and high-level GATA3 expression specifically in ILC2s, while playing only a minimal role in Th2 cell differentiation, demonstrating distinct regulatory mechanisms for the same GATA3-driven type 2 effector program in innate vs. adaptive lymphocytes. CRISPR deletion of Gata3 enhancer in mice, ILC2 and Th2 development analysis, type 2 inflammatory response assays PNAS High 34353913
2024 ILC2-specific Gata3-related tandem super-enhancers (G3SE) induce high GATA3 expression in ILC2-committed precursors. G3SE-deficient mice exhibit ILC2 deficiency with minimal impact on other ILC lineages or Th2 cells. Cnot6l (part of the CCR4-NOT deadenylase complex) is identified as a possible GATA3 target during ILC2 development. CRISPR deletion of G3SE in mice, single-cell RNA-seq, flow cytometry, developmental stage analysis Nature Communications High 38969652
2024 A distal enhancer region (mG900, ~926-970 kb downstream of GATA3) interacts with the GATA3 transcription start site in Th2 cells and is required for GATA3 expression and Th2 differentiation during house dust mite-induced allergic airway inflammation, but not during papain-induced inflammation. CRISPR deletion of mG900 region in mice, 4C-seq chromatin conformation analysis, ChIP-qPCR, allergic airway inflammation models PNAS High 38923989
2014 GATA3 loss in bladder cancer cells (siRNA knockdown) promotes cell migration and invasion, associated with increased VEGF, MMP-2, MMP-9 expression/activity, increased N-cadherin, and decreased β-catenin; enforced GATA3 expression in UMUC3 cells inhibits migration and invasion. siRNA knockdown, enforced expression, migration/invasion assays, MMP activity assays, Western blot for EMT markers Cancer Biology & Therapy Medium 24448324
2023 In hepatocytes, Gata3 regulates hepatic vascular reconstitution after hepatectomy by controlling expression of PEDF and VEGFA. Downregulation of Gata3 promotes liver sinusoidal endothelial cell proliferation and hepatic revascularization; overexpression of Gata3 leads to higher mortality in ALPPS mice, reversible by PEDF-neutralizing antibody. AAV-CRISPR screening in Alb-Cre mice with partial hepatectomy, patient-derived hepatic organoids with Gata3 inhibitor, ALPPS mouse model Journal of Hepatology Medium 37918568
2022 GATA3 and MDM2 are synthetically lethal in ER-positive breast cancer. GATA3 deficiency combined with MDM2 inhibition significantly impairs tumor growth. The synthetic lethality requires p53 and acts via the PI3K/Akt/mTOR pathway. GATA3 depletion combined with MDM2 inhibitors in vitro, in vivo, and patient-derived organoids/xenografts; p53 requirement established by genetic manipulation; pathway analysis Communications Biology High 35440675

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 GATA3 haplo-insufficiency causes human HDR syndrome. Nature 415 10935639
2013 GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression. Nature cell biology 314 23354167
2007 Notch directly regulates Gata3 expression during T helper 2 cell differentiation. Immunity 303 17658278
2010 GATA3 in development and cancer differentiation: cells GATA have it! Journal of cellular physiology 244 19798694
2019 Methodologies for Improving HDR Efficiency. Frontiers in genetics 242 30687381
2014 GATA-3 function in innate and adaptive immunity. Immunity 217 25148023
2004 Mutation of GATA3 in human breast tumors. Oncogene 216 15361840
2014 GATA3: a master of many trades in immune regulation. Trends in immunology 197 24786134
2014 Gata3 drives development of RORγt+ group 3 innate lymphoid cells. The Journal of experimental medicine 181 24419270
2013 GATA3 expression in breast carcinoma: utility in triple-negative, sarcomatoid, and metastatic carcinomas. Human pathology 158 23375642
2021 Dynamics and competition of CRISPR-Cas9 ribonucleoproteins and AAV donor-mediated NHEJ, MMEJ and HDR editing. Nucleic acids research 130 33398341
2009 GATA3 inhibits breast cancer growth and pulmonary breast cancer metastasis. Oncogene 118 19483726
2013 Critical role of p38 and GATA3 in natural helper cell function. Journal of immunology (Baltimore, Md. : 1950) 110 23851685
2015 GATA3 in Breast Cancer: Tumor Suppressor or Oncogene? Gene expression 103 26637396
2018 Divergent lncRNA GATA3-AS1 Regulates GATA3 Transcription in T-Helper 2 Cells. Frontiers in immunology 101 30420860
2015 The role of GATA3 in breast carcinomas: a review. Human pathology 89 26772397
2018 Efficient single-copy HDR by 5' modified long dsDNA donors. eLife 86 30156184
2004 GATA3 and NeuroD distinguish auditory and vestibular neurons during development of the mammalian inner ear. Mechanisms of development 81 15003631
2007 GATA-3 - not just for Th2 cells anymore. Cellular & molecular immunology 75 17349208
2008 Role of GATA-3 in allergic diseases. Current molecular medicine 71 18691059
2022 TRPS1, GATA3, and SOX10 expression in triple-negative breast carcinoma. Human pathology 65 35413381
2017 GATA3 interacts with and stabilizes HIF-1α to enhance cancer cell invasiveness. Oncogene 58 28263977
2021 GATA3 functions downstream of BRCA1 to suppress EMT in breast cancer. Theranostics 57 34373738
2001 Transcription factor GATA3 and the human HDR syndrome. Cellular and molecular life sciences : CMLS 55 11577985
1994 Structure and expression of the human GATA3 gene. Genomics 55 8088776
2022 RAD51AP1 regulates ALT-HDR through chromatin-directed homeostasis of TERRA. Molecular cell 54 36265488
2004 Partially overlapping expression of Gata2 and Gata3 during inner ear development. Developmental dynamics : an official publication of the American Association of Anatomists 51 15499560
2014 Loss of GATA3 in bladder cancer promotes cell migration and invasion. Cancer biology & therapy 50 24448324
2008 Gfi1-mediated stabilization of GATA3 protein is required for Th2 cell differentiation. The Journal of biological chemistry 50 18701459
2014 Shift in GATA3 functions, and GATA3 mutations, control progression and clinical presentation in breast cancer. Breast cancer research : BCR 49 25410484
2008 GATA-3 as a marker of hormone response in breast cancer. The Journal of surgical research 48 19059610
2021 Role of GATA3 in tumor diagnosis: A review. Pathology, research and practice 46 34547599
2018 Bcl11b, a novel GATA3-interacting protein, suppresses Th1 while limiting Th2 cell differentiation. The Journal of experimental medicine 46 29514917
2023 GATA3 mediates doxorubicin resistance by inhibiting CYB5R2-catalyzed iron reduction in breast cancer cells. Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 44 37230023
2013 A study of gata3 and phox2b expression in tumors of the autonomic nervous system. The American journal of surgical pathology 43 23715162
2015 Wnt/β-Catenin Mediates AICAR Effect to Increase GATA3 Expression and Inhibit Adipogenesis. The Journal of biological chemistry 42 26109067
2016 Function and Hormonal Regulation of GATA3 in Human First Trimester Placentation. Biology of reproduction 40 27733378
2016 GATA3 inhibits GCM1 activity and trophoblast cell invasion. Scientific reports 37 26899996
2015 GATA3 expression in gestational trophoblastic tissues and tumours. Histopathology 34 25753145
2021 A Gata3 enhancer necessary for ILC2 development and function. Proceedings of the National Academy of Sciences of the United States of America 33 34353913
2019 GATA3 recruits UTX for gene transcriptional activation to suppress metastasis of breast cancer. Cell death & disease 33 31685800
2022 Setd2 supports GATA3+ST2+ thymic-derived Treg cells and suppresses intestinal inflammation. Nature communications 32 36463230
2022 Multiplex HDR for disease and correction modeling of SCID by CRISPR genome editing in human HSPCs. Molecular therapy. Nucleic acids 32 36618262
2017 GATA3 regulates FLG and FLG2 expression in human primary keratinocytes. Scientific reports 32 28928464
2021 The estrogen receptor/GATA3/FOXA1 transcriptional network: lessons learned from breast cancer. Current opinion in structural biology 29 34225008
2016 GATA3 immunohistochemical expression in invasive urothelial carcinoma. Urologic oncology 29 27241168
2013 Gata3/Ruvbl2 complex regulates T helper 2 cell proliferation via repression of Cdkn2c expression. Proceedings of the National Academy of Sciences of the United States of America 28 24167278
2020 Sox12 enhances Fbw7-mediated ubiquitination and degradation of GATA3 in Th2 cells. Cellular & molecular immunology 27 32152552
2020 The Diagnostic Dilemma of GATA3 Immunohistochemistry in Pheochromocytoma and Paraganglioma. Endocrine pathology 27 32303954
2019 Diagnostic utility of GATA3 immunohistochemical expression in urothelial carcinoma. Indian journal of pathology & microbiology 27 30971548
2006 Involvement of GATA3 in protein kinase C theta-induced Th2 cytokine expression. European journal of immunology 27 17111354
2022 Predictable NHEJ Insertion and Assessment of HDR Editing Strategies in Plants. Frontiers in genome editing 26 35481279
2022 GATA-3 is a proto-oncogene in T-cell lymphoproliferative neoplasms. Blood cancer journal 26 36329027
2019 GATA3 Expression in Common Gynecologic Carcinomas: A Potential Pitfall. International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 26 30059453
2019 Combined use of SOX10 and GATA3 in mammary carcinoma. Pathology, research and practice 26 31924335
2023 Functional role of GATA3 and CDX2 in lineage specification during bovine early embryonic development. Reproduction (Cambridge, England) 25 36630554
2018 The multi-faceted role of Gata3 in developmental haematopoiesis. Open biology 25 30463912
2017 GATA3 and MYB Expression in Cutaneous Adnexal Neoplasms. The American Journal of dermatopathology 25 28323779
2013 Linking GATA-3 and interleukin-13: implications in asthma. Inflammation research : official journal of the European Histamine Research Society ... [et al.] 25 24363163
2018 Mutant GATA3 Actively Promotes the Growth of Normal and Malignant Mammary Cells. Anticancer research 24 30061207
2022 GATA3 and MDM2 are synthetic lethal in estrogen receptor-positive breast cancers. Communications biology 23 35440675
2016 Expression of T-bet, Eomesodermin and GATA-3 in porcine αβ T cells. Developmental and comparative immunology 23 26920461
2024 Enhancing homology-directed repair efficiency with HDR-boosting modular ssDNA donor. Nature communications 22 39122671
2023 Gene of the month: GATA3. Journal of clinical pathology 22 37726118
2017 Utility of GATA3 in the differential diagnosis of pheochromocytoma. Histopathology 22 28374498
2021 LncRNA GATA3‑AS1‑miR‑30b‑5p‑Tex10 axis modulates tumorigenesis in pancreatic cancer. Oncology reports 21 33760161
2017 GATA3 expression in triple-negative breast cancers. Histopathology 20 28211079
2025 Fine-Tuning Homology-Directed Repair (HDR) for Precision Genome Editing: Current Strategies and Future Directions. International journal of molecular sciences 18 40362308
2022 Immunohistochemical expression of renin and GATA3 help distinguish juxtaglomerular cell tumors from renal glomus tumors. Human pathology 18 35926808
2014 GATA3 expression in sarcomatoid urothelial carcinoma of the bladder. Human pathology 18 24824028
2023 Balance of Gata3 and Ramp2 in hepatocytes regulates hepatic vascular reconstitution in postoperative liver regeneration. Journal of hepatology 17 37918568
2020 High rate of HDR in gene editing of p.(Thr158Met) MECP2 mutational hotspot. European journal of human genetics : EJHG 17 32332872
2020 The GATA3 X308_Splice breast cancer mutation is a hormone context-dependent oncogenic driver. Oncogene 17 32587399
2019 GATA-3 in T-cell lymphoproliferative disorders. IUBMB life 17 31317631
2017 GATA3 expression and its relationship with clinicopathological parameters in invasive breast carcinomas. Pathology, research and practice 17 28215639
2023 METTL3 promotes SMSCs chondrogenic differentiation by targeting the MMP3, MMP13, and GATA3. Regenerative therapy 16 36793308
2022 Loss of function of GATA3 induces basal-like mammary tumors. Theranostics 16 34976209
2022 GATA3 Predicts the Tumor Microenvironment Phenotypes and Molecular Subtypes for Bladder Carcinoma. Frontiers in surgery 16 35647011
2022 Precise exogenous insertion and sequence replacements in poplar by simultaneous HDR overexpression and NHEJ suppression using CRISPR-Cas9. Horticulture research 16 36133672
2021 ILC2 Cells Promote Th2 Cell Differentiation in AECOPD Through Activated Notch-GATA3 Signaling Pathway. Frontiers in immunology 16 34354706
2021 GATA3 is essential for separating patterning domains during facial morphogenesis. Development (Cambridge, England) 16 34383890
2019 SOX10 and GATA3 in Adenoid Cystic Carcinoma and Polymorphous Adenocarcinoma. Head and neck pathology 16 31222589
2017 Regulatory network of GATA3 in pediatric acute lymphoblastic leukemia. Oncotarget 16 28415601
2024 TRPS1 and GATA3 Expression in Invasive Breast Carcinoma With Apocrine Differentiation. Archives of pathology & laboratory medicine 15 37074839
2024 A distal enhancer of GATA3 regulates Th2 differentiation and allergic inflammation. Proceedings of the National Academy of Sciences of the United States of America 15 38923989
2024 Stage-specific GATA3 induction promotes ILC2 development after lineage commitment. Nature communications 15 38969652
2023 HDR, the last enzyme in the MEP pathway, differently regulates isoprenoid biosynthesis in two woody plants. Plant physiology 15 36848194
2022 GATA3 Inhibits Viral Infection by Promoting MicroRNA-155 Expression. Journal of virology 15 35319228
2022 PRPF6 promotes metastasis and paclitaxel resistance of ovarian cancer via SNHG16/CEBPB/GATA3 axis. Oncology research 15 37303939
2021 Stratification of urothelial bladder carcinoma depending on immunohistochemical expression of GATA3 and CK5/6. Journal of immunoassay & immunochemistry 15 34106817
2019 Collaborative ISL1/GATA3 interaction in controlling neuroblastoma oncogenic pathways overlapping with but distinct from MYCN. Theranostics 15 30867811
2019 Gata3 targets Runx1 in the embryonic haematopoietic stem cell niche. IUBMB life 15 31634421
2007 Gata-3 and mammary cell fate. Breast cancer research : BCR 14 17381824
2017 GATA3 expression in benign prostate glands with radiation atypia: a diagnostic pitfall. Histopathology 13 28316088
2022 Classification of BRCA2 Variants of Uncertain Significance (VUS) Using an ACMG/AMP Model Incorporating a Homology-Directed Repair (HDR) Functional Assay. Clinical cancer research : an official journal of the American Association for Cancer Research 12 35736817
2020 Polyamines polarized Th2/Th9 cell-fate decision by regulating GATA3 expression. Archives of biochemistry and biophysics 12 32946839
2018 GATA3 acetylation at K119 by CBP inhibits cell migration and invasion in lung adenocarcinoma. Biochemical and biophysical research communications 12 29453984
2021 FOXP3 and GATA3 Polymorphisms, Vitamin D3 and Multiple Sclerosis. Brain sciences 11 33806248
2020 Incidence and Significance of GATA3 Positivity in Pancreatic Ductal Adenocarcinoma and Cholangiocarcinoma. Applied immunohistochemistry & molecular morphology : AIMM 11 31033498
2020 CDX2, SATB2, GATA3, TTF1, and PAX8 Immunohistochemistry in Krukenberg Tumors. International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 11 32045390

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