Affinage

FLNB

Filamin-B · UniProt O75369

Round 2 corrected
Length
2602 aa
Mass
278.2 kDa
Annotated
2026-04-28
130 papers in source corpus 9 papers cited in narrative 9 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

Filamin B (FLNB) is a large actin-crosslinking protein that stabilizes three-dimensional actin networks, anchors transmembrane proteins to the cytoskeleton, and scaffolds cytoplasmic signaling molecules essential for cell locomotion and fetal development (PMID:11252955, PMID:11336782). Dominant missense mutations clustering in the calponin homology 2 (CH2) domain and filamin repeats 14–15 cause gain-of-function skeletal dysplasias including Larsen syndrome, atelosteogenesis I/III, and boomerang dysplasia, while homozygous loss-of-function mutations cause autosomal recessive spondylocarpotarsal synostosis syndrome (SCT), a phenotype recapitulated by Flnb-knockout mice that develop progressive vertebral and joint fusions (PMID:14991055, PMID:16752402, PMID:17635842). Alternative splicing of FLNB exon 30, regulated by the RNA-binding proteins QKI and RBFOX1, drives epithelial-to-mesenchymal transition by releasing the transcription factor FOXC1, linking FLNB isoform switching to breast cancer mesenchymal programs (PMID:30059005).

Mechanistic history

Synthesis pass · year-by-year structured walk · 6 steps
  1. 2001 High

    Establishing the foundational biochemistry of filamins — including filamin B — as actin-crosslinkers, membrane–cytoskeleton linkers, and signaling scaffolds provided the mechanistic framework for interpreting later disease genetics.

    Evidence Integrative review of biochemical binding studies, cytoskeletal imaging, and structural analyses across multiple laboratories

    PMID:11252955 PMID:11336782

    Open questions at the time
    • Paralog-specific (FLNB vs. FLNA/FLNC) functions were not resolved
    • No in vivo genetic model yet available for FLNB
  2. 2004 High

    The discovery that FLNB mutations cause multiple distinct skeletal dysplasias (Larsen syndrome, atelosteogenesis I/III, and SCT) established FLNB as a non-redundant regulator of vertebral segmentation, joint formation, and endochondral ossification.

    Evidence Mutation screening in patient cohorts; in situ hybridization in human growth plate and mouse developing vertebral bodies

    PMID:14991055

    Open questions at the time
    • Mechanism by which specific mutations produce gain-of-function vs. loss-of-function remained unknown
    • No animal model yet to confirm loss-of-function phenotype
  3. 2005 Medium

    Identification of CH2-domain mutations (L171R, S235P) causing boomerang dysplasia expanded the allelic series and pinpointed the actin-binding domain as a recurrent hotspot for dominant skeletal phenotypes.

    Evidence Sequencing of two boomerang dysplasia patients with evolutionary conservation analysis

    PMID:15994868

    Open questions at the time
    • Only two patients identified; broader cohort confirmation needed
    • Effect of these mutations on actin-binding affinity not biochemically tested
  4. 2006 High

    Systematic genotype–phenotype mapping across Larsen syndrome and atelosteogenesis cohorts revealed that disease-causing mutations cluster non-randomly in two domains — the CH2 actin-binding domain and filamin repeats 14–15 — indicating these regions are critical for FLNB's skeletal function.

    Evidence Mutation screening by dHPLC and sequencing in independent multi-center cohorts (20 Larsen, 15 AOI/AOIII probands); statistical clustering analysis

    PMID:16648377 PMID:16752402 PMID:16801345

    Open questions at the time
    • Structural basis for why repeats 14–15 are uniquely sensitive to missense mutations not determined
    • Whether mutations alter protein folding, dimerization, or partner binding is unknown
  5. 2007 High

    A Flnb-knockout mouse phenocopied human SCT — progressive vertebral and joint fusions — and patient cells with nonsense mutations lacked filamin B protein, formally establishing loss of function as the SCT disease mechanism and demonstrating that filamin B is dispensable for initial segmentation but essential for joint maintenance.

    Evidence Flnb−/− knockout mouse; skeletal analysis, histology; Western blotting of SCT patient-derived cells

    PMID:17635842

    Open questions at the time
    • Cell-autonomous vs. non-cell-autonomous role of FLNB in chondrocytes/perichondrium not resolved
    • Downstream signaling pathways disrupted by FLNB loss not identified
  6. 2018 High

    Discovery that QKI/RBFOX1-regulated skipping of FLNB exon 30 causes EMT by releasing FOXC1 revealed a non-cytoskeletal, splice-isoform–dependent transcriptional role for FLNB in epithelial plasticity and breast cancer.

    Evidence Genome-scale expression screen, RNA-seq, eCLIP, QKI/RBFOX1 loss/gain-of-function, FOXC1 reporter/rescue assays, patient tumor analysis

    PMID:30059005

    Open questions at the time
    • Mechanism by which the exon 30-skipped FLNB isoform releases FOXC1 (direct binding vs. indirect) is not fully resolved
    • Whether FLNB splicing-driven EMT contributes to metastasis in vivo has not been tested
    • Relationship between the EMT-related splice function and the skeletal phenotypes of FLNB mutations is unexplored

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key open questions include the structural basis for domain-specific pathogenicity of FLNB mutations, the downstream signaling pathways disrupted in chondrocytes, and how the cytoskeletal-scaffolding and FOXC1-regulatory roles of FLNB are coordinated across tissues.
  • No high-resolution structure of full-length filamin B or disease-relevant domains with mutations
  • Signaling pathways downstream of FLNB in growth plate chondrocytes remain uncharacterized
  • Integration of the EMT/splicing axis with skeletal biology has not been attempted

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 2 GO:0008092 cytoskeletal protein binding 2 GO:0060090 molecular adaptor activity 2
Localization
GO:0005829 cytosol 2 GO:0005856 cytoskeleton 2
Pathway
R-HSA-1266738 Developmental Biology 2 R-HSA-1474244 Extracellular matrix organization 2
Partners
FOXC1QKIRBFOX1

Evidence

Reading pass · 9 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2004 Missense mutations in FLNB cause autosomal dominant Larsen syndrome and the perinatal lethal atelosteogenesis I and III phenotypes, while homozygous/compound heterozygous stop-codon mutations cause autosomal recessive spondylocarpotarsal syndrome (SCT). Filamin B was shown to be expressed in human growth plate chondrocytes and developing vertebral bodies in mouse, establishing an unexpected role in vertebral segmentation, joint formation, and endochondral ossification for this ubiquitously expressed cytoskeletal actin-crosslinking protein. Mutation screening (sequencing) in patient cohorts combined with in situ hybridization/expression analysis in human and mouse tissue Nature genetics High 14991055
2001 Filamins (including filamin B) function as large actin-binding proteins that stabilize three-dimensional actin webs, link actin networks to cellular membranes, anchor transmembrane proteins to the cytoskeleton, and provide a scaffold for cytoplasmic signaling proteins. They are essential for cell locomotion and fetal development. Review synthesizing biochemical binding studies, cell biology experiments (knockout/knockdown, cytoskeletal imaging), and structural analyses from multiple labs Nature reviews. Molecular cell biology High 11252955
2001 Filamins organize filamentous actin into networks and stress fibers, anchor various transmembrane proteins to the actin cytoskeleton, and serve as scaffolds for cytoplasmic signaling proteins. The three human filamin paralogs (including filamin B) share this architecture with splice variants contributing to functional complexity. Structural domain analysis, binding partner studies, and functional cell biology experiments reviewed across multiple systems Biochimica et biophysica acta High 11336782
2006 Mutations in FLNB causing Larsen syndrome cluster in two regions: the actin-binding domain (calponin homology 2 domain, CH2) and filamin repeats 13–17, with missense mutations or small in-frame deletions being the exclusive mutation type. The non-random clustering of mutations in specific functional domains indicates that disruption of actin-binding domain function and specific filamin repeat regions is mechanistically responsible for the dominant skeletal dysplasia phenotype. Mutation screening by dHPLC, direct sequencing, and restriction endonuclease digestion in 20 Larsen syndrome probands; genotype-phenotype correlation Journal of medical genetics High 16801345
2005 Mutations L171R and S235P in the calponin homology 2 (CH2) region of the actin-binding domain of filamin B cause boomerang dysplasia, expanding the phenotypic spectrum of FLNB mutations. The affected residues are evolutionarily conserved, indicating critical functional importance of this region for skeletogenesis. Mutation identification by sequencing in patients with boomerang dysplasia; evolutionary conservation analysis Journal of medical genetics Medium 15994868
2006 Mutations causing atelosteogenesis I and III cluster in two regions of filamin B: exons 2–3 encoding the CH2 domain of the actin-binding region, and exons 28–29 encoding repeats 14 and 15. This bimodal clustering of 14 novel missense mutations in 15 patients demonstrates that dysfunction of these two specific FLNB domains underlies AOI/AOIII. Mutation screening and sequencing in 15 unrelated AOI/AOIII patients; genotype-phenotype correlation Human mutation High 16752402
2007 Homozygous disruption of Flnb in mice causes progressive vertebral fusions, short stature, and carpal/sternal joint fusions phenocopying human spondylocarpotarsal synostosis syndrome (SCT). Filamin B protein was absent in cells from SCT patients with nonsense mutations, confirming loss-of-function as the disease mechanism. The Flnb-/- phenotype showed that spinal segmentation can occur without filamin B, but the protein is required for maintenance of intervertebral, carpal and sternal joints — with fusion initiating antenatally. Gene targeting/knockout mouse model (Flnb-/-); skeletal analysis, histology, Western blotting of patient-derived cells Human molecular genetics High 17635842
2018 Alternative splicing of FLNB exon 30, regulated by RNA-binding proteins QKI and RBFOX1, plays a causal role in the epithelial-to-mesenchymal transition (EMT). Specifically, skipping of FLNB exon 30 induces EMT by releasing the FOXC1 transcription factor. QKI and RBFOX1 coordinately regulate FLNB splicing, and exon 30 skipping is strongly associated with EMT gene signatures in basal-like breast cancer. Genome-scale expression screen, RNA-seq, eCLIP analysis, loss-of-function and gain-of-function experiments for QKI/RBFOX1, FOXC1 reporter/rescue assays, patient sample analysis eLife High 30059005
2006 Mutations in FLNB causing Larsen syndrome cluster in the calponin homology 2 domain, repeat 14, and repeat 15 of filamin B, confirming that as few as five of 46 coding exons harbor the majority of disease-causing variants. This non-random distribution indicates these domains are critical for filamin B's role in joint formation and skeletogenesis. Mutation screening by sequencing in five independent Larsen syndrome cases; compilation and statistical analysis of mutation clusters across two studies Journal of medical genetics Medium 16648377

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 The tandem affinity purification (TAP) method: a general procedure of protein complex purification. Methods (San Diego, Calif.) 1369 11403571
2006 A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nature biotechnology 1336 16964243
2009 Defining the human deubiquitinating enzyme interaction landscape. Cell 1282 19615732
2016 ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure. Cell 1233 26777405
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2012 The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. Molecular cell 973 22681889
2004 Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Nature biotechnology 916 15592455
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2001 Filamins as integrators of cell mechanics and signalling. Nature reviews. Molecular cell biology 825 11252955
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2008 Large-scale proteomics and phosphoproteomics of urinary exosomes. Journal of the American Society of Nephrology : JASN 607 19056867
2003 A proteomics strategy to elucidate functional protein-protein interactions applied to EGF signaling. Nature biotechnology 558 12577067
2011 Renal cyst formation in Fh1-deficient mice is independent of the Hif/Phd pathway: roles for fumarate in KEAP1 succination and Nrf2 signaling. Cancer cell 497 22014577
2011 Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation. Nature cell biology 490 21423176
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
2005 Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways. Proceedings of the National Academy of Sciences of the United States of America 383 16009940
2004 14-3-3-affinity purification of over 200 human phosphoproteins reveals new links to regulation of cellular metabolism, proliferation and trafficking. The Biochemical journal 372 14744259
2001 Structural and functional aspects of filamins. Biochimica et biophysica acta 354 11336782
2020 The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis. Nature 292 32322062
2017 Genome-wide CRISPR screen identifies HNRNPL as a prostate cancer dependency regulating RNA splicing. Proceedings of the National Academy of Sciences of the United States of America 282 28611215
2012 A high-throughput approach for measuring temporal changes in the interactome. Nature methods 273 22863883
2004 Functional proteomics mapping of a human signaling pathway. Genome research 247 15231748
1994 Homozygous human TAP peptide transporter mutation in HLA class I deficiency. Science (New York, N.Y.) 244 7517574
1998 TAP- and tapasin-dependent HLA-E surface expression correlates with the binding of an MHC class I leader peptide. Current biology : CB 228 9427624
2017 Optimized fragmentation schemes and data analysis strategies for proteome-wide cross-link identification. Nature communications 221 28524877
2014 Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes. Journal of proteomics 215 25281560
2015 ∆F508 CFTR interactome remodelling promotes rescue of cystic fibrosis. Nature 209 26618866
2009 Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6. Science signaling 207 19738201
2004 Mutations in the gene encoding filamin B disrupt vertebral segmentation, joint formation and skeletogenesis. Nature genetics 203 14991055
2007 The role of the FH1 domain and profilin in formin-mediated actin-filament elongation and nucleation. Current biology : CB 181 18160294
2011 Protein interactome reveals converging molecular pathways among autism disorders. Science translational medicine 180 21653829
1994 Functional expression and purification of the ABC transporter complex associated with antigen processing (TAP) in insect cells. FEBS letters 180 8082812
2007 Targeted inactivation of fh1 causes proliferative renal cyst development and activation of the hypoxia pathway. Cancer cell 153 17418408
2004 The ABCs of immunology: structure and function of TAP, the transporter associated with antigen processing. Physiology (Bethesda, Md.) 152 15304636
2008 Mutually exclusive interactions drive handover of mRNA from export adaptors to TAP. Proceedings of the National Academy of Sciences of the United States of America 151 18364396
2018 Veterinary pharmaceutical residues from natural water to tap water: Sales, occurrence and fate. Journal of hazardous materials 148 30179788
2001 Overexpression of TAP/p15 heterodimers bypasses nuclear retention and stimulates nuclear mRNA export. The Journal of biological chemistry 114 11259411
2003 Complex formation among the RNA export proteins Nup98, Rae1/Gle2, and TAP. The Journal of biological chemistry 113 12637516
2016 Relationship between antibiotic- and disinfectant-resistance profiles in bacteria harvested from tap water. Chemosphere 103 26966812
2006 An intron with a constitutive transport element is retained in a Tap messenger RNA. Nature 101 16971948
2018 An alternative splicing switch in FLNB promotes the mesenchymal cell state in human breast cancer. eLife 100 30059005
2002 Formation of Tap/NXT1 heterodimers activates Tap-dependent nuclear mRNA export by enhancing recruitment to nuclear pore complexes. Molecular and cellular biology 98 11739738
1999 Function of the transport complex TAP in cellular immune recognition. Biochimica et biophysica acta 95 10581370
2006 A molecular and clinical study of Larsen syndrome caused by mutations in FLNB. Journal of medical genetics 93 16801345
2019 Anthropogenic gadolinium in tap water and in tap water-based beverages from fast-food franchises in six major cities in Germany. The Science of the total environment 81 31412473
1994 Altered natural killer cell repertoire in Tap-1 mutant mice. Proceedings of the National Academy of Sciences of the United States of America 81 8022815
2019 IL-12 signaling drives the differentiation and function of a TH1-derived TFH1-like cell population. Scientific reports 78 31570752
2015 Viral inhibition of the transporter associated with antigen processing (TAP): a striking example of functional convergent evolution. PLoS pathogens 78 25880312
1998 Chimeric chemoreceptors in Escherichia coli: signaling properties of Tar-Tap and Tap-Tar hybrids. Journal of bacteriology 76 9473047
1999 Specificity of the proteasome and the TAP transporter. Current opinion in immunology 72 10322157
2001 The transporter associated with antigen processing (TAP): structural integrity, expression, function, and its clinical relevance. Molecular medicine (Cambridge, Mass.) 69 11471551
1997 Interferon-gamma rapidly increases peptide transporter (TAP) subunit expression and peptide transport capacity in endothelial cells. The Journal of biological chemistry 69 9195970
2008 Features of TAP-independent MHC class I ligands revealed by quantitative mass spectrometry. European journal of immunology 67 18446792
2005 A comparative sequence analysis reveals a common GBD/FH3-FH1-FH2-DAD architecture in formins from Dictyostelium, fungi and metazoa. BMC genomics 66 15740615
2012 Determinants of Formin Homology 1 (FH1) domain function in actin filament elongation by formins. The Journal of biological chemistry 65 22247555
2018 Identification of non-mutated neoantigens presented by TAP-deficient tumors. The Journal of experimental medicine 63 30115740
2019 Microplastics Detection in Streaming Tap Water with Raman Spectroscopy. Sensors (Basel, Switzerland) 61 31003396
2009 Strep/FLAG tandem affinity purification (SF-TAP) to study protein interactions. Current protocols in protein science 61 19688738
2011 A proteasome-dependent, TAP-independent pathway for cross-presentation of phagocytosed antigen. EMBO reports 60 22037009
1997 Tap: a novel cellular protein that interacts with tip of herpesvirus saimiri and induces lymphocyte aggregation. Immunity 60 9175835
2001 U2AF participates in the binding of TAP (NXF1) to mRNA. The Journal of biological chemistry 58 11724776
1988 T cell receptor/CD3 negative variants are unresponsive to stimulation through the Ly-6 encoded molecule, TAP. Journal of immunology (Baltimore, Md. : 1950) 58 2842394
2019 Tumor-targeted silencing of the peptide transporter TAP induces potent antitumor immunity. Nature communications 57 31434881
2012 Functional and genetic characterization of the tap efflux pump in Mycobacterium bovis BCG. Antimicrobial agents and chemotherapy 57 22232275
2005 Mutations in FLNB cause boomerang dysplasia. Journal of medical genetics 56 15994868
2021 Spotlight on TAP and its vital role in antigen presentation and cross-presentation. Molecular immunology 55 34973498
2010 Arginine methylation of REF/ALY promotes efficient handover of mRNA to TAP/NXF1. Nucleic acids research 54 20129943
2001 Enhanced expression of human ABC-transporter tap is associated with cellular resistance to mitoxantrone. FEBS letters 54 11513878
1999 Identification and characterization of a protein containing formin homology (FH1/FH2) domains. Gene 53 10352228
2007 Disruption of the Flnb gene in mice phenocopies the human disease spondylocarpotarsal synostosis syndrome. Human molecular genetics 52 17635842
2003 Fhos, a mammalian formin, directly binds to F-actin via a region N-terminal to the FH1 domain and forms a homotypic complex via the FH2 domain to promote actin fiber formation. Journal of cell science 52 14576350
2001 RNA export mediated by tap involves NXT1-dependent interactions with the nuclear pore complex. The Journal of biological chemistry 49 11579093
2007 Tandem affinity purification of functional TAP-tagged proteins from human cells. Nature protocols 48 17546005
2006 Mutations in two regions of FLNB result in atelosteogenesis I and III. Human mutation 48 16752402
2023 Tumor Area Positivity (TAP) score of programmed death-ligand 1 (PD-L1): a novel visual estimation method for combined tumor cell and immune cell scoring. Diagnostic pathology 44 37076889
2009 Genetic variants in TAP are associated with high-grade cervical neoplasia. Clinical cancer research : an official journal of the American Association for Cancer Research 44 19188174
2005 Modulation of the antigen transport machinery TAP by friends and enemies. FEBS letters 43 16359665
2021 TAP dysfunction in dendritic cells enables noncanonical cross-presentation for T cell priming. Nature immunology 41 33790474
2011 EBV protein BNLF2a exploits host tail-anchored protein integration machinery to inhibit TAP. Journal of immunology (Baltimore, Md. : 1950) 39 21296983
1987 Determination of leukocytosis in traumatic spinal tap specimens. The American journal of medicine 39 3605134
2000 Synergistic induction of the Tap-1 gene by IFN-gamma and lipopolysaccharide in macrophages is regulated by STAT1. Journal of immunology (Baltimore, Md. : 1950) 37 10975834
1979 Sucrose uptake by sugar beet tap root tissue. Plant physiology 37 16661065
2016 Arabidopsis FH1 Formin Affects Cotyledon Pavement Cell Shape by Modulating Cytoskeleton Dynamics. Plant & cell physiology 35 26738547
2003 Identification and characterization of a TAP-family gene in the lamprey. Immunogenetics 35 12679854
2003 Two highly divergent ancient allelic lineages of the transporter associated with antigen processing (TAP) gene in Xenopus: further evidence for co-evolution among MHC class I region genes. European journal of immunology 35 14579270
2007 XIST RNA exhibits nuclear retention and exhibits reduced association with the export factor TAP/NXF1. Chromosoma 33 17333237
2003 ForC, a novel type of formin family protein lacking an FH1 domain, is involved in multicellular development in Dictyostelium discoideum. Journal of cell science 33 12538772
2000 Detection of adenoviruses and enteroviruses in tap water and river water by reverse transcription multiplex PCR. Canadian journal of microbiology 33 10872077
2012 Mesoscale DNA structural changes on binding and photoreaction with Ru[(TAP)2PHEHAT]2+. Journal of the American Chemical Society 32 22667828
2020 Saline lavage after a "dry tap". The bone & joint journal 30 32475286
2014 Cowpox virus protein CPXV012 eludes CTLs by blocking ATP binding to TAP. Journal of immunology (Baltimore, Md. : 1950) 29 25024387
2006 Mutations responsible for Larsen syndrome cluster in the FLNB protein. Journal of medical genetics 29 16648377
2002 Regulation of the expression of mouse TAP-associated glycoprotein (tapasin) by cytokines. Immunology letters 29 12095710
2008 The varicellovirus UL49.5 protein blocks the transporter associated with antigen processing (TAP) by inhibiting essential conformational transitions in the 6+6 transmembrane TAP core complex. Journal of immunology (Baltimore, Md. : 1950) 28 18802093
2018 In vitro bioanalysis of drinking water from source to tap. Water research 27 29656192
2012 Importance of TAP-independent processing pathways. Molecular immunology 27 23183105
2017 Isolation and identification of Acanthamoeba strains from soil and tap water in Yanji, China. Environmental health and preventive medicine 25 29165144
2016 TAP-Dependent and -Independent Peptide Import into Dendritic Cell Phagosomes. Journal of immunology (Baltimore, Md. : 1950) 25 27664280
2021 Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway. Nucleic acids research 24 34096602
2008 Genome-wide association study identifies two novel loci containing FLNB and SBF2 genes underlying stature variation. Human molecular genetics 24 19039035
1998 TAP-independent delivery of antigenic peptides to the endoplasmic reticulum: therapeutic potential and insights into TAP-dependent antigen processing. Journal of immunotherapy (Hagerstown, Md. : 1997) 23 9551364
2019 Arabidopsis Class I Formin FH1 Relocates between Membrane Compartments during Root Cell Ontogeny and Associates with Plasmodesmata. Plant & cell physiology 22 31135031
2007 TAP expression reduces IL-10 expressing tumor infiltrating lymphocytes and restores immunosurveillance against melanoma. International journal of cancer 22 17278102
2005 Recombinant SEC14-like proteins (TAP) possess GTPase activity. Biochemical and biophysical research communications 22 15567179
2022 Blastocystis in tap water of a community in northern Thailand. Parasitology international 21 35842087
2019 RppH can faithfully replace TAP to allow cloning of 5'-triphosphate carrying small RNAs. MethodsX 21 30788220
2015 Clinical Application of Serum Tumor Abnormal Protein (TAP) in Colorectal Cancer Patients. Asian Pacific journal of cancer prevention : APJCP 21 25921156
2020 To TAP or not to TAP: alternative peptides for immunotherapy of cancer. Current opinion in immunology 20 31952027
2019 M-TAP Dance: Targeting PRMT1 and PRMT5 Family Members to Push Cancer Cells Over the Edge. Cancer cell 20 31287990
2015 Affinity Purification of Protein Complexes Using TAP Tags. Methods in enzymology 20 26096502
2013 Urinary concentration: different ways to open and close the tap. Pediatric nephrology (Berlin, Germany) 20 23736674
2012 Alteration of α-tocopherol-associated protein (TAP) expression in human breast epithelial cells during breast cancer development. Food chemistry 20 23411208
2008 Differential contribution of TAP and tapasin to HLA class I antigen expression. Immunology 20 18194274
1998 Physical association between the EBV protein EBNA-1 and P32/TAP/hyaluronectin. Journal of biomedical science 20 9678487
2018 The Vacuolar Pathway of Long Peptide Cross-Presentation Can Be TAP Dependent. Journal of immunology (Baltimore, Md. : 1950) 19 30559321
2015 NTF2-like domain of Tap plays a critical role in cargo mRNA recognition and export. Nucleic acids research 19 25628355
2013 Association of TAP gene polymorphisms and risk of cervical intraepithelial neoplasia. Disease markers 19 24288424
2003 Association of TAP and HLA-DM genes with psoriasis in Koreans. The Journal of investigative dermatology 19 12648225
2018 Identification of free-living amoebae isolated from tap water in Istanbul, Turkey. Experimental parasitology 18 30336121
2016 Biological mechanisms associated with triazophos (TAP) removal by horizontal subsurface flow constructed wetlands (HSFCW). The Science of the total environment 18 26897579
2015 Tumor abnormal protein (TAP) examination contributes to primary diagnosis of bladder cancer. International journal of clinical and experimental medicine 18 26770464
2009 Reduced expression of tocopherol-associated protein (TAP/Sec14L2) in human breast cancer. Cancer investigation 18 19909011
2024 Hepatic Klf10-Fh1 axis promotes exercise-mediated amelioration of NASH in mice. Metabolism: clinical and experimental 17 38615945
2020 Division of Labor Between Two Actin Nucleators-the Formin FH1 and the ARP2/3 Complex-in Arabidopsis Epidermal Cell Morphogenesis. Frontiers in plant science 17 32194585
2019 Tandem Affinity Purification and Mass Spectrometry (TAP-MS) for the Analysis of Protein Complexes. Current protocols in protein science 17 30706993
2017 Clinical Validation of a Serum Protein Panel (FLNA, FLNB and KRT19) for Diagnosis of Prostate Cancer. Journal of molecular biomarkers & diagnosis 17 29682400