Affinage

FIS1

Mitochondrial fission 1 protein · UniProt Q9Y3D6

Length
152 aa
Mass
16.9 kDa
Annotated
2026-06-09
100 papers in source corpus 38 papers cited in narrative 38 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 9/9 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

FIS1 is a tail-anchored outer mitochondrial membrane protein that serves as a membrane-bound adaptor coordinating organelle division, mitophagy, and apoptosis (PMID:14623186, PMID:14996942). Its cytosolic region adopts a TPR-like helical bundle whose concave and convex surfaces mediate protein interactions, while a C-terminal transmembrane segment plus a basic C-terminal patch anchors the protein and confers organelle targeting to both mitochondria and peroxisomes (PMID:14623186, PMID:16107562, PMID:28007883). FIS1 promotes mitochondrial fission upstream of or in concert with the dynamin-related GTPase Drp1: overexpression fragments mitochondria in a Drp1-dependent manner and depletion elongates the network (PMID:14996942), and FIS1 binds Drp1 directly (KD ~12–68 µM) through a conserved TPR surface gated by an N-terminal arm that interconverts between autoinhibited and active conformations (PMID:17884824, PMID:36272645, PMID:37866629). This arm-dependent autoinhibition, first defined structurally in yeast Fis1, occludes the Drp1/adaptor-binding surface, and removal of the arm or mutation of arm/SKY-insert residues tunes Drp1 and TBC1D15 recruitment (PMID:15809300, PMID:22102223, PMID:36272645, PMID:37777154). Beyond Drp1, FIS1 directly recruits the Rab-GAP TBC1D15 to mitochondria to regulate morphology, and FIS1 functions downstream of Mff to couple stress-induced fission to mitophagy and degradation (PMID:23077178, PMID:24196833). FIS1 independently drives non-canonical, PINK1/Parkin-independent mitophagy by controlling STX17 shuttling between ER and mitochondria (PMID:31053718). At ER–mitochondria contact sites FIS1 bridges to the ER protein Bap31, forming a procaspase-8-activating complex that amplifies apoptosis, and the Bap31 vDED engages the convex TPR surface (PMID:21183955, PMID:40770055). FIS1 activity is extensively regulated by post-translational modification: phosphorylation at Tyr38 by Met and at Thr34 by DNA-PKcs each increases Drp1 affinity and pathological fission (PMID:34848680, PMID:35290083); SUMOylation at K149 controls mitochondrial localization and mitophagy, reversed by SENP3/SENP1 which also redirect FIS1 toward MFN2-dependent tethering (PMID:34994490, PMID:37589160); and oxidative stress exposes Cys41 to drive disulfide-bridged homodimers that enhance Drp1 recruitment (PMID:40328741). A distinct Drp1 SWAG groove mediates the pathological FIS1–Drp1 axis separably from physiological Drp1–Mff fission, providing a pharmacological handle (PMID:37468472).

Mechanistic history

Synthesis pass · year-by-year structured walk · 30 steps
  1. 2003 High

    Established the fold and adaptor concept of human Fis1 by solving its structure, framing it as a membrane-anchored protein-interaction scaffold rather than an enzyme.

    Evidence NMR solution structure of human Fis1 cytosolic domain

    PMID:14623186

    Open questions at the time
    • No binding partner identified at the structural level
    • Function of the predicted concave hydrophobic surface untested
  2. 2004 High

    Defined FIS1 as a functional regulator of mitochondrial fission acting with Drp1, answering whether it controls organelle morphology in cells.

    Evidence Deletion mutagenesis, dominant-negative Drp1(K38A) rescue, and RNAi with microscopy

    PMID:14996942

    Open questions at the time
    • Did not establish whether FIS1 binds Drp1 directly
    • Stoichiometry and recruitment mechanism unresolved
  3. 2004 Medium

    Revealed a pro-apoptotic role for FIS1 distinct from and more potent than Drp1, separating its death function from fission per se.

    Evidence shRNA knockdown with apoptosis assays, including Opa1-depletion epistasis

    PMID:15356267

    Open questions at the time
    • Molecular mediators of the apoptotic function not identified
    • Single lab, limited conditions
  4. 2005 High

    Showed FIS1 also drives peroxisomal fission and that targeting versus fission functions are separable between its TM tail and N-terminal region.

    Evidence Differential tagging, deletion mutagenesis, siRNA, microscopy in mammalian cells

    PMID:16107562

    Open questions at the time
    • Peroxisomal partner not identified in this study
    • Whether the same Drp1 machinery operates at peroxisomes untested here
  5. 2005 High

    Identified intramolecular autoinhibition: the yeast Fis1 N-terminus folds onto the TPR concave face and gates adaptor recruitment, providing a regulatory mechanism for fission activity.

    Evidence NMR structure of yeast Fis1 plus yeast complementation assays

    PMID:15809300

    Open questions at the time
    • Whether the more flexible human Fis1 arm is autoinhibitory left open
    • Trigger that relieves autoinhibition unknown
  6. 2005 Medium

    Linked FIS1 oligomeric assembly to division activity, indicating higher-order organization is required for function.

    Evidence Blue-native PAGE, deletion mutagenesis, RNAi in rat Fis1

    PMID:15979461

    Open questions at the time
    • Composition of the ~200 kDa complex not defined
    • Whether oligomerization is homotypic or includes partners unclear
  7. 2007 High

    Provided structural and direct-binding proof that the TPR concave surface binds the Drp1 ortholog Dnm1 and that the N-terminal arm autoinhibits this >100-fold, mechanistically uniting structure with fission control.

    Evidence Yeast Fis1–Mdv1/Caf4 co-crystal structures plus in vitro Dnm1 binding and mutagenesis

    PMID:17884824 PMID:17998537

    Open questions at the time
    • Direct human Fis1–Drp1 binding not yet quantified
    • How adaptors and Dnm1 binding are coordinated in time unresolved
  8. 2007 High

    Identified Pex11pβ as a direct FIS1 partner and detected Fis1–Pex11pβ–DLP1 ternary complexes, defining the peroxisomal fission machinery.

    Evidence Subcellular fractionation, co-IP, chemical crosslinking, RNAi in mammalian cells

    PMID:17408615

    Open questions at the time
    • Binding interface on FIS1 for Pex11pβ not mapped
    • Regulation of peroxisomal versus mitochondrial partitioning unknown
  9. 2008 Medium

    Connected FIS1 to mitophagy/autophagy and long-term cell survival, distinguishing its effects on organelle function from shape.

    Evidence Yeast WHI2 epistasis genetics and Fis1 overexpression autophagy assays

    PMID:18515060 PMID:18756280

    Open questions at the time
    • Molecular link between Fis1 and the autophagy machinery not defined
    • Relevance of yeast WHI2 axis to mammals unclear
  10. 2011 High

    Defined FIS1 as a hub at ER–mitochondria contacts that nucleates a Bap31-containing apoptotic platform, explaining its pro-apoptotic activity mechanistically.

    Evidence Reciprocal co-IP, fractionation, Ca²⁺ measurements, and procaspase-8 activation assays

    PMID:21183955

    Open questions at the time
    • Interaction interface not yet structurally defined
    • How fission and apoptosis platforms are switched not resolved
  11. 2011 High

    Placed FIS1 within signaling control of fission by identifying AKAP121/Siah2 as upstream regulators of the Drp1–Fis1 interaction under hypoxia.

    Evidence Co-IP, Siah2−/− mice, cardiac ischemia and C. elegans models

    PMID:22099302

    Open questions at the time
    • Mechanism by which AKAP121 occludes Drp1–Fis1 not defined
    • Direct versus indirect AKAP121 action unresolved
  12. 2011 Medium

    Showed the FIS1 cytosolic domain can directly engage and cluster membranes, suggesting an intrinsic membrane-remodeling capacity.

    Evidence Fluorescence spectroscopy, CD, light scattering, EM with lipid vesicles in vitro

    PMID:21738650

    Open questions at the time
    • No in-cell validation of membrane clustering
    • Physiological relevance to fission untested
  13. 2012 High

    Identified TBC1D15 as a direct, Drp1-independent FIS1 effector recruited to mitochondria, broadening FIS1's role beyond Drp1-mediated fission.

    Evidence Co-IP, bacterial protein reconstitution, RNAi, microscopy

    PMID:23077178

    Open questions at the time
    • How TBC1D15 recruitment alters morphology mechanistically unclear
    • Relationship to the Drp1 pathway not fully resolved
  14. 2012 Medium

    Established multilayered control of FIS1 abundance through proteasomal degradation, translational repression, and a monomer–dimer functional switch.

    Evidence DJ-1/Akt/RNF5 degradation in MPTP mice, Foxo3a–miR-484 reporter and transgenic assays, and analytical ultracentrifugation mutants

    PMID:22510686 PMID:22789569 PMID:22871147

    Open questions at the time
    • How dimer state links to Drp1 recruitment not directly shown
    • Interplay among these regulatory layers untested
  15. 2013 High

    Showed FIS1 is one of multiple redundant Drp1 receptors and acts after Mff to couple stress-induced fission to downstream mitophagy/degradation.

    Evidence Fis1/Mff single- and double-null cells, co-IP, mitochondrial toxin and LC3 aggregation assays

    PMID:23283981 PMID:24196833

    Open questions at the time
    • Quantitative contribution of each receptor in vivo unclear
    • Molecular step linking FIS1 to degradation not defined
  16. 2013 Medium

    Linked FIS1 to cell cycle progression, placing it upstream of Plk1/FoxM1 in G2/M entry.

    Evidence shRNA knockdown, flow cytometry, and rescue overexpression

    PMID:23907611

    Open questions at the time
    • Mechanism connecting FIS1 to mitotic regulators unknown
    • Whether the effect is via fission or independent unresolved
  17. 2016 High

    Resolved how the FIS1 tail anchor achieves membrane insertion and organelle specificity, and refined the post-recruitment role of FIS1 in organizing Dnm1 puncta.

    Evidence Deep mutational scanning of the tail anchor and NMR-guided E78A mutagenesis with yeast fission assays

    PMID:27496949 PMID:28007883

    Open questions at the time
    • Targeting determinants validated chiefly in yeast
    • How FIS1 organizes puncta versus uniform Dnm1 coating mechanistically unclear
  18. 2019 High

    Defined a Drp1-independent FIS1 function: controlling STX17 ER–mitochondria shuttling to gate PINK1/Parkin-independent mitophagy.

    Evidence Super-resolution microscopy, proteomics, co-IP, STX17 oligomerization and mitophagy flux assays

    PMID:31053718

    Open questions at the time
    • How FIS1 occludes the STX17 N-terminus structurally unclear
    • Interplay with canonical PINK1/Parkin mitophagy not defined
  19. 2021 High

    Identified Tyr38 phosphorylation by Met as an activating modification that promotes Drp1 recruitment and metastasis-supporting fission.

    Evidence In vitro kinase assay, phospho-specific antibody, SIM imaging, invasion and xenograft assays

    PMID:34848680

    Open questions at the time
    • Structural basis of how pY38 enhances Drp1 binding unknown
    • Whether other RTKs write this site untested
  20. 2022 High

    Identified Thr34 phosphorylation by cytoplasmic DNA-PKcs as a fission-promoting modification driving tubular cell death in acute kidney injury.

    Evidence Co-IP, in vitro kinase assay, T34A knock-in mice, kidney function and EM

    PMID:35290083

    Open questions at the time
    • How pThr34 increases Drp1 affinity structurally unresolved
    • Relationship between pThr34 and pTyr38 regulation unknown
  21. 2022 High

    Established SUMOylation at K149 and its reversal by SENP3 as a switch controlling FIS1 mitochondrial localization and mitophagy.

    Evidence SUMOylation assays, K149R mutant, SENP3 depletion, mitophagy flux and fractionation

    PMID:34994490

    Open questions at the time
    • SUMO ligase writing K149 not identified
    • How SUMOylation alters membrane localization mechanistically unclear
  22. 2022 High

    Confirmed that human FIS1 retains arm-dependent autoinhibition conserved from yeast, with arm removal reducing Drp1 recruitment and TBC1D15 partially substituting.

    Evidence MD simulations, NMR, tryptophan fluorescence, cellular Drp1 recruitment and TBC1D15 rescue

    PMID:36272645

    Open questions at the time
    • Physiological trigger that opens the human arm undefined
    • Quantitative coupling of arm state to fission unresolved
  23. 2023 High

    Quantified direct human FIS1–Drp1 binding and mapped arm and surface residues as gain- and loss-of-function alleles, cementing the autoinhibitory model in human cells.

    Evidence NMR, DSF, microscale thermophoresis, alanine scanning, morphology assays in human cells

    PMID:37866629

    Open questions at the time
    • Affinity is weak (µM); how it is potentiated in cells unclear
    • Co-receptor contributions to functional binding not addressed
  24. 2023 High

    Provided atomic-resolution insight into the FIS1–Bap31 apoptotic interface, showing Bap31 vDED engages the convex TPR surface.

    Evidence Co-crystal structure of human Fis1 with Bap31 vDED

    PMID:40770055

    Open questions at the time
    • How procaspase-8 is positioned by the complex not structurally shown
    • Regulation switching FIS1 between fission and apoptosis platforms unclear
  25. 2023 Medium

    Defined a SWAG groove on Drp1 as the pathological FIS1-binding site, separable from physiological Drp1–Mff fission and druggable for selective inhibition.

    Evidence Peptide mapping of P110, structural modeling, small-molecule (SC9) screening, cell and endotoxemia models

    PMID:37468472

    Open questions at the time
    • Direct structure of FIS1 bound at SWAG not resolved
    • How pathological signals route FIS1 to SWAG unclear
  26. 2023 Medium

    Identified the SKY insert in the first TPR repeat as required for TBC1D15 and Drp1 recruitment via intramolecular interaction with the regulatory arm.

    Evidence Site-directed deletion/mutagenesis, co-IP, TBC1D15 recruitment assays, microscopy

    PMID:37777154

    Open questions at the time
    • Why SKY-deleted FIS1 still co-IPs DRP1 but fails to recruit it unresolved
    • Structural detail of the SKY–arm interaction lacking
  27. 2023 High

    Revealed that SENP1-mediated deSUMOylation can redirect FIS1 from fission toward MFN2/VDAC1-dependent tethering, balancing mitochondrial integrity and ER–mitochondria Ca²⁺ signaling.

    Evidence Co-IP, MFN2 oligomerization assay, FIS1 mutant knock-in mice, hypoxia/Sugen rat PH models

    PMID:37589160

    Open questions at the time
    • How deSUMOylated FIS1 promotes MFN2 oligomerization mechanistically unclear
    • Reconciliation with K149/SENP3 SUMO control not addressed
  28. 2023 Medium

    Identified K20 lactylation, driven by SIRT3/PDHA1-controlled lactate, as a pathological modification promoting excessive fission in sepsis-induced AKI.

    Evidence Lactylation site identification, PDHA1 acetylation assays, in vitro and in vivo AKI models

    PMID:37479690

    Open questions at the time
    • Lactyltransferase writing K20 not identified
    • How K20la alters FIS1 conformation/binding unknown
  29. 2024 High

    Established an oxidative-stress sensing mechanism: Cys41 exposure drives disulfide-linked FIS1 homodimers that boost Drp1 recruitment and excessive fission, and defined a small-molecule probe of the activated state.

    Evidence Activated Fis1–SP11 crystal structure, disulfide crosslinking, C41A knock-in cells, Drp1 recruitment assays

    PMID:40328741

    Open questions at the time
    • How dimerization geometrically enhances Drp1 recruitment unresolved
    • Interplay with arm autoinhibition not defined
  30. 2024 Medium

    Showed SUMOylation-dependent FIS1–TBC1D17 interaction suppresses hypoxia-induced mitophagy, with SENP3 deSUMOylation relieving the block.

    Evidence Co-IP, SUMOylation assays, SENP3 depletion, mitophagy flux in glioma patient-derived stem cells

    PMID:39638786

    Open questions at the time
    • Mechanism by which FIS1–TBC1D17 suppresses mitophagy unclear
    • Relationship to the FIS1–TBC1D15 axis not resolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the many regulatory inputs (arm autoinhibition, SKY insert, phosphorylation, SUMOylation, lactylation, Cys41 dimerization) are integrated to switch FIS1 between physiological fission, pathological fission, mitophagy, tethering, and apoptosis remains unresolved.
  • No unified structural model coupling PTMs to arm conformation and partner choice
  • Quantitative hierarchy among redundant Drp1 receptors in vivo undefined
  • Switch determining fission-platform versus Bap31 apoptotic-platform engagement unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 4 GO:0098772 molecular function regulator activity 3 GO:0008289 lipid binding 1
Localization
GO:0005783 endoplasmic reticulum 3 GO:0005777 peroxisome 2
Pathway
R-HSA-1852241 Organelle biogenesis and maintenance 3 R-HSA-8953897 Cellular responses to stimuli 3 R-HSA-9612973 Autophagy 3 R-HSA-5357801 Programmed Cell Death 2
Partners
BCAP31/BAP31DNM1L/DRP1MFN2PEX11B/PEX11PΒSENP3STX17TBC1D15TBC1D17
Complex memberships
Drp1–Fis1 fission complexFis1–Bap31 (ARCosome)Fis1–Pex11pβ–DLP1 ternary complex

Evidence

Reading pass · 38 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2003 NMR solution structure of human Fis1 revealed a TPR-like helix bundle composed of six alpha-helices forming a slightly twisted slab; the structure suggests Fis1 acts as a molecular adaptor on the mitochondrial outer membrane, with a concave hydrophobic surface predicted to mediate protein-protein interactions, and a C-terminal transmembrane segment for membrane anchoring. NMR spectroscopy Journal of molecular biology High 14623186
2004 Human Fis1 inserts into the mitochondrial outer membrane via a C-terminal transmembrane domain; this transmembrane domain together with a short basic segment is essential for mitochondrial targeting. Overexpression causes mitochondrial fragmentation suppressible by dominant-negative Drp1(K38A), and RNAi knockdown causes elongation of mitochondrial tubules, establishing hFis1 as a regulator of mitochondrial fission acting upstream of or in concert with Drp1. Deletion mutagenesis, dominant-negative Drp1 rescue, RNAi knockdown with fluorescence microscopy Journal of cell science High 14996942
2004 Down-regulation of hFis1 by shRNA inhibits apoptosis more potently than down-regulation of Drp1, and at a distinct stage. Cells depleted of Opa1 die spontaneously by a process requiring hFis1 expression, indicating Fis1 has a pro-apoptotic function that Opa1 normally counteracts. shRNA knockdown, apoptosis assays (flow cytometry, cell death quantification) Molecular biology of the cell Medium 15356267
2005 Human Fis1 localizes to peroxisomes in addition to mitochondria; the transmembrane domain and short C-terminal tail are necessary and sufficient for targeting to both organelles, whereas the N-terminal region is required for organelle fission. Fis1 overexpression promotes peroxisome division and siRNA silencing causes peroxisome tubulation, establishing a role for Fis1 in peroxisomal fission. Differential tagging, deletion mutagenesis, siRNA, fluorescence microscopy Molecular biology of the cell High 16107562
2005 Analysis of rat Fis1 by BN-PAGE revealed it forms an ~200 kDa complex at the outer mitochondrial membrane. Domain analysis showed the N-terminal and C-terminal segments are both essential for oligomeric rFis1 interaction, and the middle TPR-like domains regulate proper oligomer assembly; disruption of oligomeric assembly abolishes mitochondrial division-stimulating activity. Blue-native PAGE, deletion mutagenesis, RNAi, fluorescence microscopy Biochemical and biophysical research communications Medium 15979461
2005 NMR structure of yeast Fis1 revealed that, unlike the flexible unstructured N-terminus of human Fis1, the longer N-terminus of yeast Fis1 is fixed to the concave face of the TPR domain in an intramolecular fashion. This N-terminal segment is required for Mdv1 recruitment to mitochondria and for rescuing mitochondrial fission in fis1Δ yeast, indicating intramolecular autoinhibitory regulation of Fis1 bioactivity. NMR spectroscopy, yeast complementation assay, fluorescence microscopy The Journal of biological chemistry High 15809300
2007 Crystal structures of yeast Fis1 in complex with adaptor proteins Mdv1 and Caf4 showed these adaptors use two helices to bind both the concave and convex surfaces of the Fis1 TPR domain — a novel binding mode for TPR domains. Genetic and biochemical studies confirmed both interfaces are important for Mdv1/Caf4 binding and for mitochondrial fission in vivo. X-ray crystallography, biochemical binding assays, yeast genetics Proceedings of the National Academy of Sciences of the United States of America High 17998537
2007 Yeast Fis1 directly binds Dnm1 (the Drp1 ortholog) in a manner negatively regulated by the Fis1 N-terminal arm; the concave surface of the Fis1 TPR domain mediates Dnm1 binding; removal of the arm increases Dnm1 binding >100-fold, indicating the arm acts in an autoinhibitory manner to restrict access to the Dnm1-binding site. In vitro binding assays, mutagenesis, fluorescence microscopy The Journal of biological chemistry High 17884824
2007 Fis1 localizes to peroxisomes as well as mitochondria in mammalian cells (confirmed by subcellular fractionation). Fis1 directly interacts with the peroxisomal membrane protein Pex11pβ (co-IP and binding studies), and ternary complexes of Fis1–Pex11pβ–DLP1 were detected by chemical crosslinking; Fis1 overexpression induces peroxisome proliferation while Fis1 RNAi causes peroxisome tubulation. Subcellular fractionation, co-immunoprecipitation, chemical crosslinking, RNAi, fluorescence microscopy Experimental cell research High 17408615
2008 In yeast, deletion of FIS1 consistently selects for a secondary mutation in WHI2, which rescues the mitochondrial respiratory defect (petite formation) caused by FIS1 deficiency but causes defective growth control. The long-term survival function of Fis1 in this context appears independent of Dnm1 and Mdv1 but may be mediated through the Caf4 adaptor. Yeast genetics, tiling arrays, genomic sequencing, growth assays Cell death and differentiation Medium 18756280
2008 High-level Fis1 overexpression triggers autophagy (mitophagy) correlating with mitochondrial dysfunction rather than fragmentation per se, as demonstrated by Fis1 mutants with dissociated effects on organelle shape versus function. Fis1 overexpression/mutagenesis, autophagosome formation assays, fluorescence microscopy Biochimica et biophysica acta Medium 18515060
2010 Fis1 bridges the mitochondria–ER interface by interacting with the ER protein Bap31, facilitating Bap31 cleavage into pro-apoptotic p20Bap31; the Fis1–Bap31 complex (ARCosome) recruits procaspase-8 via the variant death effector domain (vDED) of Bap31, activating procaspase-8 and releasing ER Ca²⁺ to amplify mitochondrial apoptosis in a feedback loop. Co-immunoprecipitation, subcellular fractionation, apoptosis assays, Ca²⁺ measurements, domain deletion analysis The EMBO journal High 21183955
2011 AKAP121 inhibits Drp1–Fis1 interaction in a PKA-independent manner; reduced AKAP121 availability (via ubiquitin ligase Siah2) relieves inhibition and increases Drp1–Fis1 interaction, resulting in mitochondrial fission under hypoxia. This defines Siah2 as a key upstream regulator of Fis1/Drp1-mediated fission. Co-immunoprecipitation, genetic knockout (Siah2−/− mice), cardiac ischemia models, C. elegans lifespan assays Molecular cell High 22099302
2011 The cytosolic domain of Fis1 alone binds lipid vesicles (enhanced by protonation and anionic phospholipids), undergoes a membrane-induced conformational change burying two tryptophan residues, and promotes reversible lipid vesicle clustering, suggesting Fis1 can directly interact with membranes to facilitate fission. Fluorescence spectroscopy, circular dichroism, light scattering, electron microscopy PloS one Medium 21738650
2011 Crystal structure of yeast Fis1 at 1.75 Å resolution confirmed the TPR fold creates a concave surface important for fission that is sterically occluded by the N-terminal arm, providing a structural basis for autoinhibition. X-ray crystallography Acta crystallographica. Section F High 22102223
2012 Fis1 directly and efficiently interacts with the Rab-GAP protein TBC1D15 (but not with Drp1) at mitochondria; bacterially expressed Fis1 and TBC1D15 form a direct stable complex in vitro; TBC1D15 localizes to mitochondria only when co-expressed with Fis1, and TBC1D15 knockdown induces mitochondrial network development similar to Fis1 knockdown, indicating Fis1 recruits TBC1D15 to mitochondria to regulate morphology independently of Drp1. Co-immunoprecipitation from HeLa extracts, bacterial protein reconstitution, RNAi, fluorescence microscopy Journal of cell science High 23077178
2012 A designed point mutation A72P in Fis1 disrupts dimerization and abolishes fission activity, while two nonfunctional Fis1 variants (L80P or E78D/I85T/Y88H) are unexpectedly dimeric; these data support a model in which Fis1 activity depends on its ability to interconvert between monomer and dimer species. Analytical ultracentrifugation, mutagenesis, yeast fission assays Journal of molecular biology Medium 22789569
2012 DJ-1 promotes proteasomal degradation of Fis1 via the DJ-1/Akt/RNF5 signaling pathway: Akt1 enhances mitochondrial translocation and E3-ligase activity of RNF5, leading to Fis1 ubiquitination and degradation; DJ-1 loss increases Fis1 levels and neuronal vulnerability in an MPTP mouse model. Western blotting, co-immunoprecipitation, MPTP mouse model, RNF5 E3 ligase activity assay The Biochemical journal Medium 22871147
2012 miR-484 suppresses translation of Fis1 by binding to the amino acid coding sequence of Fis1 mRNA, inhibiting Fis1-mediated mitochondrial fission and apoptosis; Foxo3a transactivates miR-484 expression, establishing a Foxo3a–miR-484–Fis1 axis regulating mitochondrial fission. Luciferase reporter assay, miRNA overexpression/knockout mice, Western blot, apoptosis assays Nature communications Medium 22510686
2013 Both Fis1 and Mff independently contribute to mitochondrial fission and are important for the number and size of Drp1 puncta on mitochondria; either MiD49 or MiD51 can mediate Drp1 recruitment and fission in the absence of both Fis1 and Mff, demonstrating that multiple independent receptors recruit Drp1. Fis1-null, Mff-null, and Fis1/Mff double-null cell lines, immunofluorescence, Drp1 localization analysis Molecular biology of the cell High 23283981
2013 During the fission cycle, Drp1 first binds Mff, then enters a complex including Fis1 and ER proteins at the ER–mitochondrial interface; Fis1 mutations do not affect fission per se but disrupt downstream degradation events when mitochondrial toxins are used, leading to accumulation of large LC3 aggregates, indicating Fis1 acts after Mff to couple stress-induced fission with downstream mitophagy/degradation. Co-immunoprecipitation, Fis1 point mutations, mitochondrial toxin treatment, LC3 aggregation assays, ER-mitochondria interface imaging Molecular biology of the cell Medium 24196833
2013 hFis1 depletion causes a severe cell cycle progression defect through G2/M phase (~3-fold reduction in mitotic index); reintroduction of hFis1 restores the cell cycle; G2/M regulators including cyclin B1, Cdk1, Plk1, Aurora kinase A, and Mad2 are suppressed in hFis1-knockdown cells; Plk1 or FoxM1 expression (but not cyclin B1/Cdk1 complex reconstitution) restores mitotic entry, placing hFis1 upstream of Plk1/FoxM1 in G2/M regulation. shRNA knockdown, flow cytometry, Western blotting, rescue overexpression experiments Cellular and molecular life sciences Medium 23907611
2016 Deep mutational scanning of the Fis1p tail anchor in yeast showed: (1) the positively charged patch at the C-terminus is required for both membrane insertion and organelle specificity; (2) positively charged residues are more acceptable within the membrane-associated domain than negatively charged residues, providing in vivo evidence for lysine/arginine 'snorkeling'; (3) TA length changes of ±3 amino acids do not inhibit mitochondrial targeting. Next-generation sequencing-based deep mutational scanning, Gal4 reporter assay, fluorescence microscopy of individual mutants Genetics High 28007883
2016 Fis1 E78A mutation weakens interaction with Mdv1 (identified by NMR-guided pKa analysis), alters mitochondrial morphology, abolishes fission in growth assays, and causes uniform Dnm1 coating of mitochondria rather than puncta formation at low expression, supporting a post-recruitment role for Fis1 in organizing Dnm1 puncta. NMR pKa measurements, mutagenesis, yeast fission growth assay, fluorescence microscopy The Journal of biological chemistry Medium 27496949
2018 AMPK is intrinsically activated in AML leukemia stem cells and is upstream of FIS1; FIS1 mediates mitophagy activity essential for LSC self-renewal; FIS1 depletion attenuates mitophagy, inactivates GSK3, induces myeloid differentiation, cell cycle arrest, and loss of LSC self-renewal potential, establishing an AMPK→FIS1→mitophagy axis in LSC maintenance. shRNA knockdown, AMPK inhibition, mitophagy assays, colony-forming/self-renewal assays, xenograft models Cell stem cell Medium 29910151
2019 Fis1 depletion from the outer mitochondrial membrane causes aberrant STX17 (Syntaxin 17) accumulation on mitochondria; STX17–Fis1 interaction (identified by proteomics) controls dynamic shuttling of STX17 between ER and mitochondria; Fis1 loss exposes the STX17 N-terminus, promotes STX17 self-oligomerization, triggering PINK1/Parkin-independent mitophagy via ATG14 recruitment and Rab7-dependent lysosome fusion. Super-resolution microscopy (SR-SIM), proteomics, Co-IP, STX17 oligomerization assays, mitophagy flux assays, Rab7 interaction experiments Nature communications High 31053718
2021 Met receptor tyrosine kinase directly phosphorylates Fis1 at Tyr38 (Fis1 pY38); Fis1 pY38 promotes mitochondrial fission by recruiting Drp1 to mitochondria; fragmented mitochondria fuel actin remodeling and lamellipodia/invadopodia formation to facilitate HCC cell metastasis in vitro and in vivo. Structured illumination microscopy, live-cell imaging, in vitro kinase assay, phospho-specific antibody, Drp1 recruitment assays, invasion/migration assays, xenograft models Signal transduction and targeted therapy High 34848680
2022 DNA-PKcs translocates to the cytoplasm during AKI, directly interacts with Fis1, and phosphorylates Fis1 at Thr34 (TQ motif); Fis1 pThr34 increases affinity of Fis1 for Drp1, inducing mitochondrial fragmentation and tubular cell death; knock-in T34A mice are protected from AKI and show reduced mitochondrial fragmentation. Co-immunoprecipitation, in vitro kinase assay, phospho-specific detection, T34A knockin mice, kidney function assays, electron microscopy Science signaling High 35290083
2022 Fis1 is SUMOylated at K149, an amino acid residue critical for its mitochondrial localization; SENP3 (stabilized by CHIP downregulation upon DFP treatment) deSUMOylates Fis1, enhancing Fis1 mitochondrial localization; SENP3 depletion abolishes DFP-induced mitophagy; a K149R mutation (preventing SUMOylation) enhances mitochondrial Fis1 localization and restores DFP-induced mitophagy in SENP3-depleted cells. SUMOylation assays, site-directed mutagenesis (K149R), SENP3 depletion, mitophagy flux assays, fractionation EMBO reports High 34994490
2022 NMR and X-ray structural studies showed the human Fis1 arm can adopt an intramolecular conformation akin to yeast Fis1; removal of the Fis1 arm reduces Drp1 recruitment and mitochondrial fission in cells similar to yeast; TBC1D15 expression partially rescues arm-less Fis1 function, reminiscent of Mdv1 in yeast, supporting conserved arm-dependent regulation across eukaryotes. Molecular dynamics simulations, NMR, intrinsic tryptophan fluorescence, cellular DRP1 recruitment assays, TBC1D15 rescue experiments The Journal of biological chemistry High 36272645
2023 Human Fis1 directly binds human Drp1 (KD = 12–68 μM) as measured by NMR, differential scanning fluorimetry, and microscale thermophoresis; similar to yeast, interaction is governed by the Fis1 N-terminal arm and a conserved surface; alanine scanning of the arm identified loss-of-function (N6A → elongated mitochondria) and gain-of-function (E7A → fragmented mitochondria) alleles; Y76A substitution also caused fragmented mitochondria, supporting intramolecular arm–surface interactions regulating Drp1-mediated fission. NMR, differential scanning fluorimetry, microscale thermophoresis, alanine scanning mutagenesis, mitochondrial morphology assays in human cells The Journal of biological chemistry High 37866629
2023 A switch I-adjacent groove (SWAG) on Drp1 is the binding site for the Fis1-derived peptide inhibitor P110; small molecules binding SWAG (e.g., SC9) mimic P110's benefit by selectively inhibiting pathological Drp1–Fis1-mediated fission without blocking physiological Drp1–Mff-mediated fission in cells and in a mouse endotoxemia model. Peptide mapping, structural modeling, small molecule screening, cellular Drp1–Fis1 interaction assays, mouse endotoxemia model Nature communications Medium 37468472
2023 Crystal structure of human Fis1 bound to the C-terminal Bap31_vDED region showed that the Bap31 vDED region engages the convex surface of Fis1's TPR domain; one Fis1 conformation features the N-terminal arm (suggestive of autoinhibition); this provides structural basis for Fis1–Bap31 interaction at ER–mitochondria contact sites. X-ray crystallography (co-crystal structure), structural analysis Communications biology High 40770055
2023 A conserved three-residue SKY insert (S45, K46, Y47) in FIS1's first TPR repeat is required for TBC1D15 and DRP1 recruitment to mitochondria; deletion of the SKY insert elongates mitochondrial networks with reduced mitochondrial DRP1 despite enriched DRP1 co-immunoprecipitates; the SKY insert mediates intramolecular interactions with the regulatory Fis1 arm to govern FIS1 activity. Site-directed mutagenesis/deletion, co-immunoprecipitation, fluorescence microscopy, YFP-TBC1D15 recruitment assays The Journal of biological chemistry Medium 37777154
2023 SENP1 translocates to endothelial mitochondria under short-term hypoxia and deSUMOylates FIS1; deSUMOylated FIS1 assembles with MFN2 and VDAC1, enhancing MFN2 oligomerization and membrane tethering activity, thereby maintaining mitochondrial integrity and ER–mitochondria Ca²⁺ communication; prolonged hypoxia reduces SENP1 availability via miR-138 induction, causing SUMOylated FIS1 accumulation and mitochondrial dysfunction leading to pulmonary hypertension. Co-immunoprecipitation, MFN2 oligomerization assay, viral-packaged FIS1 mutant knock-in mice, Ca²⁺ communication assays, hypoxia/Sugen rat PH models Circulation research High 37589160
2023 Lactylation of Fis1 at lysine 20 (Fis1 K20la), driven by excess lactate produced via SIRT3-mediated PDHA1 hyperacetylation/inactivation, promotes excessive mitochondrial fission leading to ATP depletion, mtROS overproduction, and mitochondrial apoptosis in sepsis-induced AKI; reducing lactate (via DCA or SIRT3 overexpression) decreases Fis1 K20la and alleviates AKI. Lactylation site identification, PDHA1 acetylation assays, in vitro and in vivo AKI models, mitochondrial morphology assays Cell death & disease Medium 37479690
2024 Under oxidative stress, Fis1 undergoes a conformational change exposing its only cysteine Cys41 (in the α1 helix); exposed Cys41 forms disulfide-bridged Fis1 homodimers that increase Drp1 recruitment to mitochondria and excessive fission; a small molecule SP11 binds specifically to activated (Cys41-exposed) Fis1, blocking homodimerization and preserving mitochondrial integrity; cells lacking Cys41 (C41A) show reduced stress-induced fission. Structural analysis (activated Fis1–SP11 crystal structure), disulfide crosslinking, C41A knockin cell lines, Drp1 recruitment assays, mitochondrial morphology under oxidative stress Nature communications High 40328741
2024 FIS1 deSUMOylation by SENP3 interacts with TBC1D17 (a Rab-GAP) in a SUMOylation-dependent manner under hypoxia; hypoxia promotes FIS1 SUMO2/3-ylation, triggering FIS1–TBC1D17 interaction that suppresses hypoxia-induced mitophagy; SENP3-mediated deSUMOylation of FIS1 promotes hypoxia-induced mitophagy and protects against hypoxic cell death. Co-immunoprecipitation, SUMOylation assays, SENP3 depletion, mitophagy flux assays, glioma patient-derived stem cells Cell death & disease Medium 39638786

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2013 Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in mitochondrial fission. Molecular biology of the cell 1008 23283981
2004 Roles of the mammalian mitochondrial fission and fusion mediators Fis1, Drp1, and Opa1 in apoptosis. Molecular biology of the cell 897 15356267
2010 Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform for apoptosis induction. The EMBO journal 434 21183955
2004 Levels of human Fis1 at the mitochondrial outer membrane regulate mitochondrial morphology. Journal of cell science 281 14996942
2005 A role for Fis1 in both mitochondrial and peroxisomal fission in mammalian cells. Molecular biology of the cell 262 16107562
2013 Adaptor proteins MiD49 and MiD51 can act independently of Mff and Fis1 in Drp1 recruitment and are specific for mitochondrial fission. The Journal of biological chemistry 248 23921378
2006 Formation of elongated giant mitochondria in DFO-induced cellular senescence: involvement of enhanced fusion process through modulation of Fis1. Journal of cellular physiology 241 16883569
2018 AMPK/FIS1-Mediated Mitophagy Is Required for Self-Renewal of Human AML Stem Cells. Cell stem cell 240 29910151
2010 Decreased expression of Drp1 and Fis1 mediates mitochondrial elongation in senescent cells and enhances resistance to oxidative stress through PINK1. Journal of cell science 214 20179104
2011 Fine-tuning of Drp1/Fis1 availability by AKAP121/Siah2 regulates mitochondrial adaptation to hypoxia. Molecular cell 212 22099302
2008 High levels of Fis1, a pro-fission mitochondrial protein, trigger autophagy. Biochimica et biophysica acta 204 18515060
2012 miR-484 regulates mitochondrial network through targeting Fis1. Nature communications 192 22510686
2013 Mutations in Fis1 disrupt orderly disposal of defective mitochondria. Molecular biology of the cell 181 24196833
2019 Drp1/Fis1 interaction mediates mitochondrial dysfunction in septic cardiomyopathy. Journal of molecular and cellular cardiology 164 30981733
2023 PDHA1 hyperacetylation-mediated lactate overproduction promotes sepsis-induced acute kidney injury via Fis1 lactylation. Cell death & disease 153 37479690
2018 Inhibition of Drp1/Fis1 interaction slows progression of amyotrophic lateral sclerosis. EMBO molecular medicine 153 29335339
2003 The solution structure of human mitochondria fission protein Fis1 reveals a novel TPR-like helix bundle. Journal of molecular biology 134 14623186
2007 Fis1, DLP1, and Pex11p coordinately regulate peroxisome morphogenesis. Experimental cell research 128 17408615
2012 Fis1 acts as a mitochondrial recruitment factor for TBC1D15 that is involved in regulation of mitochondrial morphology. Journal of cell science 124 23077178
2017 Drp1/Fis1 interaction mediates mitochondrial dysfunction, bioenergetic failure and cognitive decline in Alzheimer's disease. Oncotarget 120 29464060
2007 Structural basis for recruitment of mitochondrial fission complexes by Fis1. Proceedings of the National Academy of Sciences of the United States of America 115 17998537
2019 STX17 dynamically regulated by Fis1 induces mitophagy via hierarchical macroautophagic mechanism. Nature communications 114 31053718
2021 Fis1 phosphorylation by Met promotes mitochondrial fission and hepatocellular carcinoma metastasis. Signal transduction and targeted therapy 113 34848680
2015 Drp1, Mff, Fis1, and MiD51 are coordinated to mediate mitochondrial fission during UV irradiation-induced apoptosis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 100 26432782
2022 DNA-PKcs interacts with and phosphorylates Fis1 to induce mitochondrial fragmentation in tubular cells during acute kidney injury. Science signaling 95 35290083
2017 Ischemia-induced Drp1 and Fis1-mediated mitochondrial fission and right ventricular dysfunction in pulmonary hypertension. Journal of molecular medicine (Berlin, Germany) 94 28265681
2020 FIS1 encodes a GA2-oxidase that regulates fruit firmness in tomato. Nature communications 77 33203832
2015 miR-483-5p determines mitochondrial fission and cisplatin sensitivity in tongue squamous cell carcinoma by targeting FIS1. Cancer letters 77 25843291
2005 Analysis of functional domains of rat mitochondrial Fis1, the mitochondrial fission-stimulating protein. Biochemical and biophysical research communications 64 15979461
2018 Drp1/Fis1-mediated mitochondrial fragmentation leads to lysosomal dysfunction in cardiac models of Huntington's disease. Journal of molecular and cellular cardiology 61 30550751
2008 Fis1 deficiency selects for compensatory mutations responsible for cell death and growth control defects. Cell death and differentiation 60 18756280
2024 Inhibition of Drp1- Fis1 interaction alleviates aberrant mitochondrial fragmentation and acute kidney injury. Cellular & molecular biology letters 58 38439028
2005 Novel structure of the N terminus in yeast Fis1 correlates with a specialized function in mitochondrial fission. The Journal of biological chemistry 57 15809300
2023 Targeting an allosteric site in dynamin-related protein 1 to inhibit Fis1-mediated mitochondrial dysfunction. Nature communications 54 37468472
2007 Direct binding of the dynamin-like GTPase, Dnm1, to mitochondrial dynamics protein Fis1 is negatively regulated by the Fis1 N-terminal arm. The Journal of biological chemistry 54 17884824
2006 Dimeric Dnm1-G385D interacts with Mdv1 on mitochondria and can be stimulated to assemble into fission complexes containing Mdv1 and Fis1. The Journal of biological chemistry 53 16601120
2020 Downregulation of Drp1 and Fis1 Inhibits Mitochondrial Fission and Prevents High Glucose-Induced Apoptosis in Retinal Endothelial Cells. Cells 52 32664237
2022 Long-term cadmium exposure impairs cognitive function by activating lnc-Gm10532/m6A/FIS1 axis-mediated mitochondrial fission and dysfunction. The Science of the total environment 48 36336035
2022 The SUMO protease SENP3 regulates mitochondrial autophagy mediated by Fis1. EMBO reports 42 34994490
2011 Fis1, Bap31 and the kiss of death between mitochondria and endoplasmic reticulum. The EMBO journal 42 21285974
2012 DJ-1 promotes the proteasomal degradation of Fis1: implications of DJ-1 in neuronal protection. The Biochemical journal 40 22871147
2008 Peroxisome fission in Hansenula polymorpha requires Mdv1 and Fis1, two proteins also involved in mitochondrial fission. Traffic (Copenhagen, Denmark) 39 18513378
2023 Endothelial FIS1 DeSUMOylation Protects Against Hypoxic Pulmonary Hypertension. Circulation research 38 37589160
2013 Human mitochondrial Fis1 links to cell cycle regulators at G2/M transition. Cellular and molecular life sciences : CMLS 38 23907611
2021 miR-379 deletion ameliorates features of diabetic kidney disease by enhancing adaptive mitophagy via FIS1. Communications biology 37 33398021
2022 Mitochondrial fission mediated by Drp1-Fis1 pathway and neurodegenerative diseases. Reviews in the neurosciences 33 36059131
2019 Fis1 deficiencies differentially affect mitochondrial quality in skeletal muscle. Mitochondrion 33 31526891
2018 Melatonin activates FIS1, DYN1, and DYN2 Plasmodium falciparum related-genes for mitochondria fission: Mitoemerald-GFP as a tool to visualize mitochondria structure. Journal of pineal research 32 29480948
2021 The mitochondrial fission factor FIS1 promotes stemness of human lung cancer stem cells via mitophagy. FEBS open bio 31 34051059
2011 Charcot-Marie-Tooth-related gene GDAP1 complements cell cycle delay at G2/M phase in Saccharomyces cerevisiae fis1 gene-defective cells. The Journal of biological chemistry 30 21890626
2023 Human Fis1 directly interacts with Drp1 in an evolutionarily conserved manner to promote mitochondrial fission. The Journal of biological chemistry 29 37866629
2020 Identification of Fis1 Interactors in Toxoplasma gondii Reveals a Novel Protein Required for Peripheral Distribution of the Mitochondrion. mBio 29 32047127
2016 Precise expression of Fis1 is important for glucose responsiveness of beta cells. The Journal of endocrinology 28 27179109
2022 PEX11β and FIS1 cooperate in peroxisome division independently of mitochondrial fission factor. Journal of cell science 27 35678336
2019 SS-31 reduces inflammation and oxidative stress through the inhibition of Fis1 expression in lipopolysaccharide-stimulated microglia. Biochemical and biophysical research communications 27 31582222
2018 Hyperglycaemia Stress-Induced Renal Injury is Caused by Extensive Mitochondrial Fragmentation, Attenuated MKP1 Signalling, and Activated JNK-CaMKII-Fis1 Biological Axis. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 23 30504726
2022 Hepatic Fis1 regulates mitochondrial integrated stress response and improves metabolic homeostasis. JCI insight 22 35015731
2024 Targeting DNM1L/DRP1-FIS1 axis inhibits high-grade glioma progression by impeding mitochondrial respiratory cristae remodeling. Journal of experimental & clinical cancer research : CR 21 39350223
2021 Loss of Fis1 impairs proteostasis during skeletal muscle aging in Drosophila. Aging cell 21 34061429
2020 Selective brain hypothermia-induced neuroprotection against focal cerebral ischemia/reperfusion injury is associated with Fis1 inhibition. Neural regeneration research 21 31719256
2016 Evidence for Amino Acid Snorkeling from a High-Resolution, In Vivo Analysis of Fis1 Tail-Anchor Insertion at the Mitochondrial Outer Membrane. Genetics 21 28007883
1996 Identification of new Fis binding sites by DNA scission with Fis-1,10-phenanthroline-copper(I) chimeras. Biochemistry 21 8605181
2022 Lanthanum decreased VAPB-PTPP51, BAP31-FIS1, and MFN2-MFN1 expression of mitochondria-associated membranes and induced abnormal autophagy in rat hippocampus. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 20 35090998
2018 Effect of Resveratrol on Sirtuins, OPA1, and Fis1 Expression in Adult Zebrafish Retina. Investigative ophthalmology & visual science 20 30208422
2022 Structural studies of human fission protein FIS1 reveal a dynamic region important for GTPase DRP1 recruitment and mitochondrial fission. The Journal of biological chemistry 19 36272645
2019 Genome-wide synthetic lethal CRISPR screen identifies FIS1 as a genetic interactor of ALS-linked C9ORF72. Brain research 19 31843624
2019 Sirtuin 3 inhibition induces mitochondrial stress in tongue cancer by targeting mitochondrial fission and the JNK-Fis1 biological axis. Cell stress & chaperones 18 30656603
2024 Lipotoxicity-induced upregulation of FIS1 exacerbates mitochondrial fragmentation and promotes NLRP3-dependent pyroptosis in diabetic cardiomyopathy. Free radical biology & medicine 17 39734056
2022 Iron chelation promotes mitophagy through SENP3-mediated deSUMOylation of FIS1. Autophagy 17 35275026
2021 FIS1 Overexpression Is Correlated with Tumor Metastasis in Gastric Adenocarcinoma. Journal of gastrointestinal cancer 17 33890233
2011 The cytosolic domain of Fis1 binds and reversibly clusters lipid vesicles. PloS one 17 21738650
2021 Dynamic interplay between the co-opted Fis1 mitochondrial fission protein and membrane contact site proteins in supporting tombusvirus replication. PLoS pathogens 16 33725015
2024 SIRT1 regulates mitochondrial fission to alleviate high altitude hypoxia inducedcardiac dysfunction in rats via the PGC-1α-DRP1/FIS1/MFF pathway. Apoptosis : an international journal on programmed cell death 15 38678130
2020 Dispensable Role of Mitochondrial Fission Protein 1 (Fis1) in the Erythrocytic Development of Plasmodium falciparum. mSphere 15 32968006
2018 Phosphatidylinositol 3-kinase-mediated HO-1/CO represses Fis1 levels and alleviates lipopolysaccharide-induced oxidative injury in alveolar macrophages. Experimental and therapeutic medicine 15 30210614
2016 Carbon monoxide alleviates lipopolysaccharide-induced oxidative stress injury through suppressing the expression of Fis1 in NR8383 cells. Experimental cell research 14 27751838
2005 A rust-inducible gene from flax (fis1) is involved in proline catabolism. Planta 13 16079997
2024 SENP3-FIS1 axis promotes mitophagy and cell survival under hypoxia. Cell death & disease 11 39638786
2016 A Targeted Mutation Identified through pKa Measurements Indicates a Postrecruitment Role for Fis1 in Yeast Mitochondrial Fission. The Journal of biological chemistry 11 27496949
2012 A designed point mutant in Fis1 disrupts dimerization and mitochondrial fission. Journal of molecular biology 11 22789569
2009 Evidence for conformational heterogeneity of fission protein Fis1 from Saccharomyces cerevisiae. Biochemistry 9 19522466
2025 A hidden cysteine in Fis1 targeted to prevent excessive mitochondrial fission and dysfunction under oxidative stress. Nature communications 8 40328741
2024 NOTCH localizes to mitochondria through the TBC1D15-FIS1 interaction and is stabilized via blockade of E3 ligase and CDK8 recruitment to reprogram tumor-initiating cells. Experimental & molecular medicine 7 38409448
2023 A conserved, noncanonical insert in FIS1 mediates TBC1D15 and DRP1 recruitment for mitochondrial fission. The Journal of biological chemistry 7 37777154
2023 Selective inhibitors targeting Fis1/Mid51 protein-protein interactions protect against hypoxia-induced damage in cardiomyocytes. Frontiers in pharmacology 7 38192411
2011 The 1.75 Å resolution structure of fission protein Fis1 from Saccharomyces cerevisiae reveals elusive interactions of the autoinhibitory domain. Acta crystallographica. Section F, Structural biology and crystallization communications 7 22102223
2024 Mitochondrial FIS1 As a Novel Drug Target for the Treatment of Erectile Dysfunction: A Multi-Omic and Epigenomic Association Study. The world journal of men's health 6 39478651
2016 Transcriptomic analysis reveals sex-specific differences in the expression of Dcl1 and Fis1 genes in the radio-adaptive response of thymocytes to TRP53-mediated apoptosis. BMC genomics 6 27581076
2012 Mitochondrial fission proteins Fis1 and Mdv1, but not Dnm1, play a role in maintenance of heteroplasmy in budding yeast. FEBS letters 6 22575664
2024 Biogenic selenium nanoparticles alleviate intestinal barrier injury in mice through TBC1D15/Fis1/Rab7 pathway. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 5 38749178
2018 Molecular Characterisation and Functions of Fis1 and PDCD6 Genes from Echinococcus granulosus. International journal of molecular sciences 5 30205566
2018 The Mitochondrial Genes BAK1, FIS1 and SFN are Linked with Alterations in Mitochondrial Membrane Potential in Barrett's Esophagus. International journal of molecular sciences 4 30404157
2025 BMAL1 modulation alleviates inflammatory responses in monocytes by targeting the Fis1-mediated mitochondrial unfolded protein response in high-altitude hypoxia. Cell communication and signaling : CCS 3 41063179
2025 Mitochondrial dysfunction in mesenchymal stem cells impairs osteogenesis in radiation-induced bone injury via Ca2+-NFATc1-Fis1 pathway. Cell death & disease 3 41331010
2025 CUMS stress facilitates hippocampal neural mitophagy through FIS1/MFF-mediated mitochondrial fragmentation. Journal of neurophysiology 2 40748742
2025 Crystal structure of Fis1 and Bap31 provides information on protein-protein interactions at mitochondria-associated ER membranes. Communications biology 2 40770055
2025 Tumor peripheral stiffness modulates lenvatinib resistance in HCC preclinical models by regulating FIS1-dependent mitophagy. JHEP reports : innovation in hepatology 2 41334455
2022 Modeling Adsorption, Conformation, and Orientation of the Fis1 Tail Anchor at the Mitochondrial Outer Membrane. Membranes 2 36005667
2025 Pharmacological and Genetic Approaches to Downregulate FIS1 Mitigate Neuropathic Pain. The Journal of neuroscience : the official journal of the Society for Neuroscience 1 40769725
2025 The role of FIS1 and its post-translational modifications in diseases and health damage caused by environmental pollution. Cell biology and toxicology 1 41351740

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