Affinage

SENP3

Sentrin-specific protease 3 · UniProt Q9H4L4

Length
574 aa
Mass
65.0 kDa
Annotated
2026-06-10
100 papers in source corpus 43 papers cited in narrative 43 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

SENP3 is a SUMO2/3-specific cysteine isopeptidase that deconjugates SUMO from substrate proteins and is the human counterpart of the yeast protease Ulp1, whose crystallized covalent transition-state complex with the SUMO ortholog Smt3 defined the catalytic and SUMO-recognition determinants of this protease family (PMID:10882122, PMID:11029585). The enzyme is constitutively short-lived: under basal conditions the co-chaperone E3 ligase CHIP (STUB1) ubiquitinates SENP3 for proteasomal degradation, while reactive oxygen species drive thiol modification of specific cysteines that recruits Hsp90, protecting SENP3 from CHIP and stabilizing the protein (PMID:19680224, PMID:20924358). This redox switch makes SENP3 a ROS sensor that accumulates and redistributes from the nucleolus to the nucleoplasm and cytoplasm, with its nucleolar tethering controlled by NPM1 binding through an N-terminal domain that requires mTOR-mediated phosphorylation (PMID:11029585, PMID:19680224, PMID:25288641). In the nucleolus SENP3, together with NPM1, deSUMOylates NPM1 itself to drive 28S rRNA maturation and ribosome biogenesis (PMID:18259216, PMID:19015314). Once stabilized and relocalized, SENP3 acts on a broad substrate range to control diverse processes: it deSUMOylates the HIF-1 co-activator p300 in a redox-tuned manner to set HIF-1 transcriptional output (PMID:19680224, PMID:22684029); it deSUMOylates the fission GTPase Drp1 and the fission protein FIS1 to govern mitochondrial fragmentation, apoptotic cytochrome c release, and mitophagy (PMID:23524851, PMID:28262828, PMID:34994490, PMID:39638786); it regulates chromatin through deSUMOylation of MLL-complex subunit RbBP5 and the methyltransferases SETD7, controlling H3K4 methylation, HOX/DLX3 transcription, and lineage differentiation (PMID:24930734, PMID:31141694); and it modulates innate and adaptive immunity by deSUMOylating MKK7, IFI204, BACH2, and IRF8 to tune JNK/TLR4 signaling, STING-dependent interferon responses, Treg stability, and osteoclastogenesis (PMID:30089837, PMID:29352108, PMID:32049023, PMID:33434504). Its catalytic activity is further gated through the cell cycle by inhibitory CDK1 phosphorylation reversed by PP1α, linking SENP3 to mitotic chromosome stability and the p53-controlled G2 checkpoint (PMID:29438989, PMID:32351703).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 2000 High

    Established the catalytic basis of the SENP/Ulp protease family: a single enzyme both matures SUMO precursors and deconjugates SUMO from substrates, and the structural determinants of SUMO recognition were defined.

    Evidence Crystal structure of the Ulp1-Smt3 covalent transition-state complex with in vitro proteolytic assays and yeast genetics

    PMID:10882122

    Open questions at the time
    • Yeast ortholog rather than human SENP3
    • Did not address substrate selectivity in mammalian cells
  2. 2000 High

    Identified human SENP3 (SMT3IP1) as a SUMO2/3-preferring isopeptidase and localized its nucleolar targeting to an N-terminal sequence, distinguishing it from ubiquitin/Nedd8 proteases.

    Evidence Yeast two-hybrid cloning, baculovirus expression, in vitro isopeptidase assays on RanGAP1 conjugates, and localization

    PMID:11029585

    Open questions at the time
    • In vitro substrate specificity may not reflect cellular targets
    • Mechanism of nucleolar retention not defined
  3. 2003 High

    Showed that subcellular localization, imposed by the non-catalytic N-domain, is a physiological constraint on which SUMO substrates the protease can access.

    Evidence Domain deletion, in vitro cleavage, in vivo conjugate profiling, and karyopherin interaction mapping in yeast Ulp1

    PMID:12471376 PMID:12654900

    Open questions at the time
    • Yeast tethering uses karyopherins/NPC; human SENP3 uses a distinct NPM1-based mechanism
    • Direct mammalian carryover not demonstrated here
  4. 2008 High

    Defined SENP3's first concrete cellular role: NPM1-associated deSUMOylation required for 28S rRNA maturation and ribosome biogenesis, with NPM1 also stabilizing the protein.

    Evidence Co-IP, in vitro desumoylation of NPM1-SUMO2, siRNA knockdown with rRNA processing readouts in mammalian and Xenopus systems

    PMID:18259216 PMID:18948745 PMID:19015314

    Open questions at the time
    • Full set of nucleolar substrates beyond NPM1 not defined
    • ARF/p53-independent contributions only partially resolved
  5. 2009 High

    Identified SENP3 as a ROS-responsive enzyme that is stabilized and relocalized by oxidative stress, linking redox state to HIF-1 transcriptional output via p300 deSUMOylation.

    Evidence Cycloheximide chase, proteasome inhibition, immunofluorescence redistribution, co-IP, luciferase reporter, and xenograft

    PMID:19680224 PMID:22684029

    Open questions at the time
    • Which cysteines sense ROS clarified later (#12) but full redox proteomics absent
    • Quantitative thresholds of ROS sensing not established
  6. 2010 High

    Resolved the molecular basis of SENP3 turnover: CHIP drives constitutive ubiquitination/degradation, while ROS-induced thiol modification recruits Hsp90 to protect SENP3.

    Evidence Co-IP, in-cell ubiquitination assays, CHIP knockdown, Hsp90 inhibition, and redox thiol modification assays

    PMID:20181954 PMID:20924358

    Open questions at the time
    • How Hsp90 binding sterically blocks CHIP not structurally defined
    • Other E3 ligases for SENP3 not excluded
  7. 2013 High

    Connected SENP3 to mitochondrial fission and cell death by identifying Drp1 as a substrate whose deSUMOylation promotes mitochondrial recruitment, cytochrome c release, and caspase activation.

    Evidence OGD ischemia model, PERK/cathepsin B inhibition, co-IP, mitochondrial fractionation, and cytochrome c/caspase assays

    PMID:23524851 PMID:28262828 PMID:34722538

    Open questions at the time
    • Stress-specific degradation pathways (PERK/cathepsin B vs CHIP) not unified
    • Quantitative apoptotic threshold control unclear
  8. 2014 High

    Demonstrated SENP3 controls chromatin and differentiation by deSUMOylating MLL-complex subunit RbBP5 and being targeted via NPM1/mTOR-regulated nucleolar tethering.

    Evidence Co-IP with MLL complexes, ChIP, in vivo SUMOylation, osteogenic differentiation assays, and mTOR inhibitor/domain-mapping experiments

    PMID:15 PMID:24930734 PMID:25288641 PMID:28344658 PMID:31141694

    Open questions at the time
    • How mTOR phosphorylation toggles between rRNA and chromatin functions not resolved
    • Breadth of chromatin substrates incomplete
  9. 2018 High

    Established SENP3 as a redox-gated regulator of immunity, deSUMOylating MKK7, BACH2, and later IFI204 and IRF8 to control inflammatory signaling, Treg stability, interferon responses, and osteoclastogenesis.

    Evidence Lineage-specific conditional Senp3 knockouts (myeloid, Treg, DC), co-IP, SUMOylation assays, and in vivo inflammation/tumor models

    PMID:29352108 PMID:30089837 PMID:32049023 PMID:33434504

    Open questions at the time
    • Substrate selection across immune contexts not mechanistically unified
    • How localization changes dictate immune-specific substrates unclear
  10. 2018 Medium

    Showed SENP3 catalytic activity is gated through mitosis by inhibitory CDK1 phosphorylation (reversed by PP1α) and by p53 during the G2 checkpoint, linking it to chromosome stability and APC/C control.

    Evidence Kinase/phosphatase identification, phospho-mutant analysis, co-IP, chromosome instability and G2 arrest assays

    PMID:29438989 PMID:32351703 PMID:35869062

    Open questions at the time
    • Single-lab phospho-mutant studies
    • Structural basis of phospho-regulation of catalysis not defined
  11. 2022 High

    Extended SENP3's mitochondrial role to mitophagy and identified FIS1 K149 deSUMOylation, with structural evidence that the Brucella effectors NyxA/NyxB sequester SENP3 through a defined acidic patch.

    Evidence FIS1 K149R mutagenesis, mitophagy/hypoxia assays, and crystal structure of NyxB with localization/epistasis experiments

    PMID:34994490 PMID:36609656 PMID:39638786

    Open questions at the time
    • How SENP3 distinguishes fission vs mitophagy outcomes on FIS1/Drp1 unclear
    • Pathogen-driven sequestration physiological scope limited
  12. 2025 Medium

    Expanded the substrate repertoire to stability control (RACK1, CTH, β-catenin, Sp1, STAT3), showing SENP3 deSUMOylation can either stabilize or destabilize targets to influence translation, metabolism, and disease.

    Evidence Co-IP, SUMOylation/stability assays, site-directed mutagenesis, and conditional knockouts in HCC, AAA, and vascular remodeling models

    PMID:26511642 PMID:27181202 PMID:34000626 PMID:39755756 PMID:40019399

    Open questions at the time
    • Why deSUMOylation stabilizes some substrates but destabilizes others not mechanistically generalized
    • Most studies single-lab in disease-specific contexts

Open questions

Synthesis pass · forward-looking unresolved questions
  • How SENP3 selects among its very broad substrate set across compartments and stress states—and whether localization, phosphorylation, redox state, and partner availability constitute a unified targeting code—remains unresolved.
  • No systematic substrate-targeting model
  • No structure of full-length human SENP3
  • Compartment-specific interactomes incompletely mapped

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 6 GO:0098772 molecular function regulator activity 5 GO:0016787 hydrolase activity 3
Localization
GO:0005730 nucleolus 6 GO:0005739 mitochondrion 4 GO:0005829 cytosol 2 GO:0005654 nucleoplasm 1
Pathway
R-HSA-168256 Immune System 5 R-HSA-1640170 Cell Cycle 4 R-HSA-392499 Metabolism of proteins 3 R-HSA-4839726 Chromatin organization 3 R-HSA-8953897 Cellular responses to stimuli 3 R-HSA-9612973 Autophagy 3 R-HSA-5357801 Programmed Cell Death 2 R-HSA-8953854 Metabolism of RNA 2
Partners
DRP1FIS1HSP90MKK7NPM1P300RBBP5STUB1
Complex memberships
MLL1/MLL2 histone methyltransferase complexchromosomal passenger complexrixosome

Evidence

Reading pass · 43 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 Ulp1 (yeast ortholog of SENP3) catalyzes two distinct SUMO pathway reactions: (1) processing of full-length SUMO precursor to its mature form, and (2) deconjugation of SUMO from targeted proteins. A covalent thiohemiacetal transition state complex between Ulp1 C-terminal fragment and Smt3 was captured and its crystal structure solved, revealing determinants of SUMO recognition. An N-terminal regulatory element (N-terminal to the proteolytic domain) is required for cell growth in yeast. Crystal structure of Ulp1-Smt3 covalent transition-state complex; in vitro proteolytic assays; genetic analysis Molecular cell High 10882122
2000 SMT3IP1 (human SENP3) was cloned via yeast two-hybrid with Smt3b as bait. Baculovirus-expressed SMT3IP1 cleaves SUMO-1 and Smt3b from RanGAP1 conjugates in vitro, with slightly stronger activity toward Smt3b (SUMO-2/3) conjugates. The enzyme binds Smt3a and Smt3b more strongly than SUMO-1 in vitro. SMT3IP1 does not cleave Nedd8 or ubiquitin from their conjugates. The N-terminal sequence is responsible for nucleolar localization of the enzyme. Yeast two-hybrid cloning; baculovirus expression; in vitro isopeptidase assay on RanGAP1 conjugates; in vitro binding assays; cell fractionation/immunofluorescence for localization European journal of biochemistry High 11029585
2003 The N-terminal domain of yeast Ulp1 is necessary and sufficient to concentrate Ulp1 at nuclear envelope (NPC) sites, while the Ulp domain (UD, ~200 residues) alone supports wild-type growth and can cleave SUMO from substrates in vitro. The N-terminal domain restricts Ulp1 activity toward certain sumoylated proteins while enabling cleavage of others, indicating subcellular localization as a physiologically significant constraint on substrate specificity. N-terminally deleted Ulp1 can suppress defects of ulp2Δ cells, unlike full-length Ulp1. Domain deletion analysis; in vitro SUMO cleavage assay; in vivo SUMO conjugate profiling; genetic complementation/suppression assays The Journal of cell biology High 12654900
2003 Yeast Ulp1 is tethered to nuclear pores via its non-catalytic N-domain, which associates with three karyopherins (Pse1, Kap95, and Kap60) in a complex not dissociated by RanGTP in vitro. The Ulp1 N-domain has two distinct binding sites for Pse1 and Kap95/Kap60, both required for NPC anchoring. The catalytic C-domain of Ulp1 must be excluded from the nucleoplasm for cell viability. Co-immunoprecipitation; mass spectrometry interactome; in vitro RanGTP dissociation assay; domain mapping; genetic viability assays Nature cell biology High 12471376
2008 SENP3 is a nucleolar SUMO-specific protease associated with nucleophosmin (NPM1). SENP3 catalyzes desumoylation of NPM1-SUMO2 conjugates in vitro and counteracts ARF-induced SUMO2 modification of NPM1 in vivo. Depletion of SENP3 by siRNA inhibits conversion of 32S rRNA to 28S rRNA, phenocopying NPM1 depletion. Constitutive SUMO2 modification of NPM1 interferes with 28S rRNA maturation, defining SENP3 as an essential factor for ribosome biogenesis. Co-immunoprecipitation; in vitro desumoylation assay; siRNA knockdown; rRNA processing assay (Northern blot/metabolic labeling) EMBO reports High 18259216
2008 SENP3 is a specific interaction partner of Borealin (a chromosomal passenger complex component) and catalyzes removal of SUMO2/3 from Borealin. The SUMO E3 ligase RanBP2 interacts with the CPC, stimulates SUMO modification of Borealin in vitro, and is required for its modification in vivo. This defines a mitotic SUMO2/3 conjugation-deconjugation cycle on Borealin regulated by RanBP2 and SENP3. Co-immunoprecipitation; in vitro SUMOylation assay with RanBP2; in vivo SUMOylation analysis; siRNA knockdown; mitotic synchronization Molecular biology of the cell High 18946085
2008 B23/nucleophosmin binds SENP3 and SENP5 in Xenopus laevis egg extracts and is essential for stable accumulation of SENP3 and SENP5 in mammalian tissue culture cells. Codepletion of SENP3 and SENP5, or depletion of B23/nucleophosmin, causes accumulation of SUMO proteins within nucleoli and defects in ribosome biogenesis. Co-immunoprecipitation in Xenopus extracts; siRNA depletion; SUMO localization by immunofluorescence; ribosome biogenesis assays The Journal of cell biology High 19015314
2008 ARF triggers sequential phosphorylation, polyubiquitination, and rapid proteasomal degradation of Senp3 in an NPM-dependent manner. Stabilization of both ARF and Senp3 requires NPM; viable Npm-null cells destabilize both proteins. NPM mutants retaining acidic and oligomerization domains can re-stabilize Senp3. Knockdown of Senp3 mimics the antiproliferative functions of ARF, suggesting SENP3 antagonism mediates p53-independent tumor-suppressive functions of ARF. shRNA knockdown; NPM-null MEFs; Western blot for ubiquitination and phosphorylation; NPM domain mutant rescue experiments Cell cycle (Georgetown, Tex.) Medium 18948745
2009 SENP3 protein is continuously degraded through the ubiquitin-proteasome pathway under basal conditions; reactive oxygen species (ROS) inhibit this degradation and stabilize SENP3. ROS also causes SENP3 to redistribute from the nucleolus to the nucleoplasm. Stabilized/redistributed SENP3 de-conjugates SUMO2/3 from the HIF-1α co-activator p300 (not HIF-1α itself), which enhances p300 binding to HIF-1α and increases HIF-1 transcriptional activity. Cycloheximide chase; proteasome inhibitor treatment; immunofluorescence for subcellular redistribution; siRNA knockdown; co-immunoprecipitation; luciferase reporter; in vivo xenograft The EMBO journal High 19680224
2010 SENP3 stability is regulated by interplay between the co-chaperone/E3 ubiquitin ligase CHIP and Hsp90. Under non-stress conditions, CHIP mediates Hsp90-independent ubiquitination and degradation of SENP3. Upon mild oxidative stress, SENP3 undergoes thiol modification, which recruits Hsp90; Hsp90/SENP3 association protects SENP3 from CHIP-mediated ubiquitination, but this protection requires the presence of CHIP. Enhanced SENP3/Hsp90 association is found in cancer cells. Co-immunoprecipitation; ubiquitination assay; Hsp90 inhibitor treatment; redox thiol modification assay; Western blot stability assay The EMBO journal High 20924358
2010 Low-dose H2O2 induces SENP3 protein accumulation and causes SENP3 to co-localize with PML bodies, resulting in de-conjugation of SUMO2/3 from PML. DeSUMOylation of PML by SENP3 reduces PML body number and is responsible for accelerated cell proliferation under mild oxidative stress; only SUMOylated PML exerts an inhibitory role on proliferation. H2O2 treatment; immunofluorescence co-localization; SUMO2/3 de-conjugation assay; siRNA knockdown; SUMOylation-deficient PML mutant rescue The Journal of biological chemistry Medium 20181954
2011 SMT3IP1/SENP3 interacts with p53 and Mdm2, and desumoylates both proteins. Overexpression of SMT3IP1 causes Mdm2 accumulation in the nucleolus, increases p53 stability, and suppresses Mdm2-mediated p53 ubiquitination and degradation. SMT3IP1 competes with p53 for binding to the acidic domain of Mdm2. Notably, the desumoylation activity of SMT3IP1 is not required for p53 stabilization. Co-immunoprecipitation; overexpression; in vivo ubiquitination assay; nucleolar localization by immunofluorescence; catalytic mutant analysis Biochemical and biophysical research communications Medium 21316347
2012 The biphasic redox regulation of HIF-1 transcriptional activity by H2O2 is mediated by differential cysteine modification of SENP3. ROS levels differentially modify cysteines 243 and 532 in SENP3, regulating its interaction with p300 to cause differential p300 SUMOylation and thereby shifting HIF-1 transcriptional activity (enhanced at low ROS, suppressed at high ROS). Dose-response H2O2 treatment; luciferase reporter; co-immunoprecipitation; chromatin immunoprecipitation; site-directed mutagenesis of SENP3 cysteines Acta pharmacologica Sinica Medium 22684029
2013 SENP3 is degraded during oxygen/glucose deprivation (OGD, an ischemia model) via a pathway involving the UPR kinase PERK and lysosomal enzyme cathepsin B. A key target for SENP3-mediated deSUMOylation is the GTPase Drp1. SENP3 depletion prolongs Drp1 SUMOylation, suppressing Drp1 localization at mitochondria, cytochrome c release, and caspase-mediated cell death. SENP3 recovery upon reoxygenation enables Drp1 deSUMOylation and promotes mitochondrial fragmentation and cell death. RNAi knockdown; OGD model; PERK inhibitor; cathepsin B inhibitor; co-immunoprecipitation; mitochondrial fractionation; cytochrome c release assay; caspase activity assay The EMBO journal High 23524851
2014 SENP3 controls H3K4 methylation by regulating MLL1/MLL2 histone methyltransferase complexes. SENP3 associates with MLL1/MLL2 complexes and catalyzes deSUMOylation of RbBP5. This is required for activation of HOX genes including DLX3, as SENP3 absence impairs menin and Ash2L association with the DLX3 gene, decreases H3K4 methylation, and reduces RNA polymerase II recruitment. The SENP3-DLX3 pathway governs osteogenic differentiation of human stem cells. Co-immunoprecipitation; ChIP; in vivo SUMOylation assay; siRNA knockdown; osteogenic differentiation assay; H3K4 methylation analysis Molecular cell High 24930734
2014 mTOR kinase pathway controls the nucleolar targeting of SENP3 by regulating its interaction with NPM1. An N-terminal domain of SENP3 is defined as the critical NPM1 binding region; mTOR-mediated phosphorylation of serine/threonine residues within this region fosters the SENP3-NPM1 interaction. Inhibition of mTOR triggers nucleolar release of SENP3, compromising its activity in rRNA processing. mTOR inhibitor (rapamycin/Torin) treatment; domain mapping; co-immunoprecipitation; phosphorylation analysis; rRNA processing assay Molecular and cellular biology Medium 25288641
2014 SENP3 promotes epithelial-mesenchymal transition (EMT) in gastric cancer via deSUMOylation of the transcription factor FOXC2. FOXC2 is identified as a SENP3 substrate; deSUMOylation of FOXC2 by SENP3 enhances its transcriptional activity toward N-cadherin. ROS-induced deSUMOylation of FOXC2 is blocked by silencing SENP3. Co-immunoprecipitation; in vivo SUMOylation assay; siRNA knockdown and overexpression; EMT marker analysis; migration assay; nude mouse model Oncotarget Medium 25216525
2015 SENP3 antagonizes SUMO2/3-targeted ubiquitination of Sp1 mediated by the SUMO-targeted ubiquitin E3 ligase RNF4. DeSUMOylation of Sp1 by SENP3 attenuates Sp1 interaction with RNF4, preventing its proteasomal degradation and increasing Sp1 protein levels. Co-immunoprecipitation; ubiquitination assay; siRNA knockdown; Western blot stability assay Protein & cell Medium 26511642
2016 SENP3 enhances STAT3 phosphorylation at Y705 by de-conjugating SUMO2/3 from STAT3 at K451. SUMO2/3 modification of STAT3 at K451 facilitates STAT3 binding to the phosphatase TC45 through a SUMO-interacting motif of TC45, thereby dephosphorylating STAT3. SENP3-mediated deSUMOylation of STAT3 prevents TC45 binding and thus enhances STAT3 phosphorylation. Co-immunoprecipitation; site-directed mutagenesis (K451); siRNA knockdown; phosphorylation assay; tobacco extract treatment Oncogene Medium 27181202
2017 SENP3-mediated deSUMOylation of Drp1 selectively promotes Drp1 binding to the mitochondrial outer membrane adaptor protein Mff. Preventing Drp1 SUMOylation (SUMO acceptor site mutants) enhances Mff binding; increasing SUMOylation by SENP3 knockdown reduces Drp1-Mff binding and stress-induced cytochrome c release. Direct tethering of Drp1 to the mitochondrial outer membrane occludes the effect of SENP3 overexpression. Co-immunoprecipitation; SUMO acceptor site mutagenesis; siRNA knockdown; SENP3 overexpression; cytochrome c release assay; mitochondrial tethering construct Scientific reports Medium 28262828
2017 FLII (flightless-I homolog, a gelsolin family actin-remodeling protein) associates with SENP3 and the MLL1/2 complex, determines SENP3 recruitment and MLL1/2 complex assembly at the DLX3 gene, and is required for H3K4 methylation, active RNA polymerase II loading, and osteogenic differentiation. Co-immunoprecipitation; ChIP; siRNA knockdown; osteogenic differentiation assay Epigenetics & chromatin Medium 28344658
2018 SENP3 maintains Treg cell stability and function by controlling SUMOylation and nuclear localization of BACH2. SENP3-mediated deSUMOylation of BACH2 prevents its nuclear export, thereby repressing effector T cell differentiation genes and stabilizing Treg gene signatures. Treg-specific deletion of Senp3 results in T cell activation and autoimmune symptoms. ROS-driven SENP3 accumulation in the tumor microenvironment contributes to Treg-mediated tumor immunosuppression. Conditional Senp3 knockout (Treg-specific); co-immunoprecipitation; nuclear/cytoplasmic fractionation; SUMOylation assay; gene expression profiling Nature communications High 30089837
2018 SENP3 deficiency in myeloid cells markedly compromises TLR4 inflammatory signaling and JNK phosphorylation in response to LPS. MKK7, which selectively phosphorylates JNK, is a SENP3 substrate; SENP3-mediated deSUMOylation of MKK7 favors its binding to JNK. ROS-dependent SENP3 accumulation and MKK7 deSUMOylation occur rapidly after LPS stimulation. Conditional Senp3 KO in myeloid cells; LPS-induced inflammation model; co-immunoprecipitation; JNK phosphorylation assay; septic shock model The Journal of biological chemistry High 29352108
2018 Mitotic phosphorylation of SENP3 by Cyclin B-CDK1 suppresses SENP3 deSUMOylation activity toward chromosome-associated proteins including topoisomerase IIα (TopoIIα). Protein phosphatase 1α (PP1α) is the phosphatase that reverses this modification. SENP3 phosphorylation decreases its interaction with TopoIIα. Non-phosphorylatable SENP3 mutant causes mitotic arrest, increased chromosomal instability, and tumorigenesis promotion. Kinase identification (CDK1); phosphatase identification (PP1α); co-immunoprecipitation; phospho-mutant analysis; chromosome instability assay; tumorigenesis assay Cancer research Medium 29438989
2019 SENP3 deSUMOylates BECN1 (beclin 1) at K380, a SUMO3-conjugation site mediated by PIAS3. BECN1 SUMOylation enhances autophagosome formation by facilitating BECN1 interaction with UVRAG, PIK3C3, and ATG14, promoting PIK3C3 activity. SENP3 deSUMOylates BECN1 to impair BECN1-PIK3C3 complex formation, suppressing PIK3C3 activity and autophagy. Liver-specific Senp3 KO mice show increased autophagy under basal and fasting conditions. Liver-specific conditional KO; co-immunoprecipitation; SUMOylation assay; PIK3C3 activity assay; autophagic flux measurement; site-directed mutagenesis (K380) Autophagy High 31373534
2019 SENP3 is associated with the SETD7 histone methyltransferase and deSUMOylates SETD7. In muscle cells, SENP3 recruits SETD7 to the sarcomeric MyHC-II gene, promotes SETD7 association with active RNA polymerase II, and precludes the opposing methyltransferase Suv39h1. SENP3 is degraded in cachexia, leading to perturbed MyHC-II expression and disorganized sarcomeres. Co-immunoprecipitation; ChIP; in vivo SUMOylation assay; siRNA knockdown; muscle cell contractility assay; cachexia model Cell reports Medium 31141694
2019 SUMO1 catalyzes NLRP3 SUMOylation at K204, which facilitates ASC oligomerization, inflammasome activation, and IL-1β secretion. SENP3 is required for deSUMOylation of NLRP3 and, upon deSUMOylation, attenuates ASC recruitment, speck formation, inflammasome activation, and IL-1β cleavage. Co-immunoprecipitation; SUMOylation assay; siRNA knockdown; ASC speck formation assay; IL-1β ELISA; site-directed mutagenesis (K204) FASEB journal Medium 31914638
2020 p53 suppresses SENP3 phosphorylation in response to DNA damage at G2/M phase. Suppression of SENP3 phosphorylation activates SENP3 deSUMOylation of Cdh1, which promotes Cdh1 dephosphorylation by Cdc14B, activates APC/CCdh1 E3 ligase, and leads to ubiquitination and degradation of Plk1 in the G2 checkpoint pathway. Co-immunoprecipitation; SUMOylation assay; phosphorylation analysis; CDK1/CDK2 inhibitors; Cdc14B phosphatase assay; Plk1 ubiquitination assay; G2 arrest assay Cell discovery Medium 32351703
2020 SENP3 loss in bone marrow-derived monocytes promotes osteoclast differentiation. Mechanistically, loss of SENP3 increases SUMO3 modification of IRF8 at K310, which upregulates NFATc1 expression and osteoclastogenesis. Myeloid-specific SENP3 KO mice exhibit more severe bone loss after ovariectomy. Conditional SENP3 KO (myeloid); SUMOylation assay; site-directed mutagenesis (K310); NFATc1 expression analysis; osteoclast differentiation assay; ovariectomy model Cell reports High 32049023
2021 SENP3 senses ROS in dendritic cells (DCs) to facilitate STING-dependent antitumor immunity. DC-derived ROS trigger SENP3 accumulation and the SENP3-IFI204 interaction; SENP3 catalyzes IFI204 deSUMOylation to boost STING signaling activation. DC-specific deletion of Senp3 blunts STING-dependent type-I interferon signaling and dampens antitumor immune responses. DC-specific conditional Senp3 KO; co-immunoprecipitation; IFI204 SUMOylation assay; STING signaling assay; IFN-I measurement; tumor growth model Molecular cell High 33434504
2021 SENP3 loss in macrophages promotes M2 polarization by causing enhanced Akt1 SUMOylation and hyper-phosphorylation/activation of Akt1. Macrophage-specific deletion of SENP3 in vivo accelerates breast cancer malignancy. Conditional SENP3 KO (macrophage-specific); co-immunoprecipitation; Akt1 SUMOylation assay; Akt1 phosphorylation assay; M2 polarization assay; orthotopic tumor model Molecular oncology Medium 33932085
2021 SENP3 interacts with β-catenin and inhibits its proteasome-dependent degradation via de-SUMOylation of β-catenin, promoting VSMC proliferation and migration and vascular remodeling. SENP3+/- mice exhibit alleviated vascular remodeling. Co-immunoprecipitation; in vivo deSUMOylation assay; siRNA knockdown/overexpression; VSMC proliferation/migration assay; SENP3 heterozygous KO mouse model EBioMedicine Medium 34000626
2021 SENP3 promotes Drp1 binding to Bcl-xL at the mitochondrial outer membrane in a manner primed by Mff. DeSUMOylation of Drp1 by SENP3 promotes the Drp1-Bcl-xL interaction in vivo and in vitro. Mff and Bcl-xL can interact directly through their transmembrane domains independent of Drp1. SENP3 loss during OGD correlates with reduced Drp1-Bcl-xL interaction, contributing to cell death after reoxygenation. Co-immunoprecipitation; in vitro binding assay; SUMO acceptor site mutants; OGD/reoxygenation model; Bcl-xL transmembrane domain mutant Frontiers in cell and developmental biology Medium 34722538
2022 SENP3 is responsible for deSUMOylation of mitochondrial fission protein Fis1 at K149 (a residue critical for Fis1 mitochondrial localization). DFP treatment stabilizes SENP3 via downregulation of the E3 ubiquitin ligase CHIP. SENP3-mediated Fis1 deSUMOylation enhances Fis1 mitochondrial targeting and is required for DFP-induced mitophagy. Fis1 K149R mutation (preventing SUMOylation) enhances mitochondrial localization and restores mitophagy in SENP3-depleted cells. siRNA knockdown; co-immunoprecipitation; Fis1 K149R site-directed mutant; mitophagy assay; mitochondrial fractionation; CHIP Western blot EMBO reports High 34994490
2022 TIP60 is hyper-SUMOylated under normal conditions; upon irradiation-induced DNA damage, SENP3-mediated deSUMOylation of TIP60 promotes its interaction with DNA-PKcs to form the TIP60-DNA-PKcs complex. This is required for TIP60-mediated acetylation of DNA-PKcs and DNA-PKcs autophosphorylation, enabling NHEJ-mediated DNA damage repair. SENP3 knockdown impairs DNA damage repair. Co-immunoprecipitation; TIP60/DNA-PKcs acetylation assay; NHEJ reporter system; comet assay; γH2AX immunofluorescence; clonogenic survival assay MedComm Medium 35356800
2021 SENP3 and SENP5 deSUMOylate Aurora A (AurA) at K258, where SUMO2 conjugation in early mitosis promotes AurA kinase activity and facilitates binding with its activator Bora. Knockdown of SENP3 and SENP5 increases AurA SUMOylation, leading to increased kinase activity and abnormalities in spindle assembly and chromosome segregation; these defects are rescued by suppressing AurA kinase activity. In vivo and in vitro SUMOylation assay; site-directed mutagenesis (K258); co-immunoprecipitation; kinase activity assay; spindle assembly checkpoint assay; siRNA knockdown Journal of cell science Medium 34313310
2022 Mitotic activation of SENP3 (by prevention of inhibitory phosphorylation) in tumor cells increases micronuclei formation, which activates cGAS signaling-dependent innate immune response and increases CD8+ T cell infiltration. p53 responding to DNA damage activates mitotic SENP3 by inhibiting its phosphorylation, further increasing cellular senescence and innate immune response. Phospho-mutant SENP3 expression; micronuclei quantification; cGAS-STING pathway assay; immune-competent mouse tumor model; CD8+ T cell flow cytometry Cell death & disease Medium 35869062
2023 The enzymatic activity of SENP3 is required for deSUMOylation of rixosome subunits, which is necessary for association of the rixosome with PRC1 and for silencing of Polycomb target genes. Both SENP3 and USP7 enzymatic activities are required for Polycomb- and rixosome-dependent silencing at an ectopic reporter locus. Co-immunoprecipitation; SUMOylation assay; siRNA knockdown with catalytic mutant rescue; reporter silencing assay; ChIP Cell reports Medium 37014752
2023 The Brucella effectors NyxA and NyxB directly interact with SENP3 via a defined acidic patch (identified by crystal structure of NyxB) and prevent nucleolar localization of SENP3 at late stages of infection. By sequestering SENP3, the effectors promote cytoplasmic accumulation of nucleolar AAA-ATPase NVL and ribosomal protein RPL5. SENP3 normally regulates the subcellular localization of these nucleolar proteins through its SUMO protease activity; the Beclin1 and PIAS3 were also found to be required for the effector-dependent shuttling. Crystal structure of NyxB-acidic patch; co-immunoprecipitation; SENP3 localization by immunofluorescence; NVL/RPL5 localization analysis; genetic epistasis with Beclin1 and PIAS3 Nature communications High 36609656
2023 SENP3-mediated deSUMOylation of c-Jun activates its transcriptional activity and the MAPK/AP-1 signaling pathway in microglia following cerebral ischemia. Microglia-specific SENP3 knockdown reduces neuroinflammation, infarct volume, and neurological deficits after ischemic stroke. Co-immunoprecipitation; deSUMOylation assay; microglia-specific KD; cytokine measurement; infarct volume measurement iScience Medium 37332598
2024 SENP3 deSUMOylates RACK1, increasing RACK1 stability and its interaction with PKCβII, thereby promoting eIF4E phosphorylation and translation of oncogenes (Bcl2, Snail, Cyclin D1) in hepatocellular carcinoma. SENP3 also promotes CCL20 translation via the RACK1/eIF4E axis, facilitating tumor-associated macrophage infiltration and immune evasion. Co-immunoprecipitation; SUMOylation assay; RACK1 stability assay; eIF4E phosphorylation assay; liver-specific SENP3 KD; chemically induced HCC model Cell death and differentiation Medium 39755756
2024 SENP3 promotes hypoxia-induced mitophagy (HIM) through deSUMOylation of FIS1. Under hypoxia, FIS1 SUMO2/3-ylation promotes interaction with Rab GAP protein TBC1D17, which suppresses HIM. SENP3-mediated FIS1 deSUMOylation counteracts TBC1D17-mediated suppression, promoting mitophagy and cell survival under hypoxia. Co-immunoprecipitation; FIS1 SUMOylation assay; TBC1D17 interaction assay; mitophagy assay; hypoxia model; siRNA knockdown; glioblastoma patient-derived cultures Cell death & disease Medium 39638786
2025 SENP3 deSUMOylates CTH (Cystathionine Gamma-Lyase) at K361, facilitating its proteasome-dependent degradation. SUMO-3 modification of CTH at K361 promotes CTH protein stability. SENP3-mediated CTH degradation reduces H2S production, promoting macrophage ferroptosis and inflammation in abdominal aortic aneurysm. SENP3 expression is negatively regulated by the E3 ubiquitin ligase STUB1/CHIP. Co-immunoprecipitation; SUMOylation assay; site-directed mutagenesis (K361); CTH stability assay; myeloid-specific SENP3 KO; AAA mouse models (AngII and CaCl2); ferroptosis assay Advanced science Medium 40019399

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast. Molecular cell 607 10882122
2013 SENP3-mediated deSUMOylation of dynamin-related protein 1 promotes cell death following ischaemia. The EMBO journal 196 23524851
2009 SENP3 is responsible for HIF-1 transactivation under mild oxidative stress via p300 de-SUMOylation. The EMBO journal 183 19680224
2003 The Ulp1 SUMO isopeptidase: distinct domains required for viability, nuclear envelope localization, and substrate specificity. The Journal of cell biology 170 12654900
2000 A novel mammalian Smt3-specific isopeptidase 1 (SMT3IP1) localized in the nucleolus at interphase. European journal of biochemistry 139 11029585
2008 The nucleolar SUMO-specific protease SENP3 reverses SUMO modification of nucleophosmin and is required for rRNA processing. EMBO reports 138 18259216
2007 Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processes. Molecular biology of the cell 120 17538013
2018 SENP3 maintains the stability and function of regulatory T cells via BACH2 deSUMOylation. Nature communications 117 30089837
2003 Unconventional tethering of Ulp1 to the transport channel of the nuclear pore complex by karyopherins. Nature cell biology 111 12471376
2010 Redox regulation of the stability of the SUMO protease SENP3 via interactions with CHIP and Hsp90. The EMBO journal 109 20924358
2008 RanBP2 and SENP3 function in a mitotic SUMO2/3 conjugation-deconjugation cycle on Borealin. Molecular biology of the cell 97 18946085
2021 SENP3 senses oxidative stress to facilitate STING-dependent dendritic cell antitumor function. Molecular cell 96 33434504
2008 Nucleolar protein B23/nucleophosmin regulates the vertebrate SUMO pathway through SENP3 and SENP5 proteases. The Journal of cell biology 95 19015314
2010 SENP3-mediated de-conjugation of SUMO2/3 from promyelocytic leukemia is correlated with accelerated cell proliferation under mild oxidative stress. The Journal of biological chemistry 92 20181954
2016 SUMOylation and SENP3 regulate STAT3 activation in head and neck cancer. Oncogene 76 27181202
2021 SENP3 loss promotes M2 macrophage polarization and breast cancer progression. Molecular oncology 75 33932085
2000 Yeast Ulp1, an Smt3-specific protease, associates with nucleoporins. Journal of biochemistry 70 11056382
2017 SENP3-mediated deSUMOylation of Drp1 facilitates interaction with Mff to promote cell death. Scientific reports 65 28262828
2013 The nuclear pore regulates GAL1 gene transcription by controlling the localization of the SUMO protease Ulp1. Molecular cell 63 24074957
2019 A fine-tuning mechanism underlying self-control for autophagy: deSUMOylation of BECN1 by SENP3. Autophagy 62 31373534
2014 The SUMO-specific isopeptidase SENP3 regulates MLL1/MLL2 methyltransferase complexes and controls osteogenic differentiation. Molecular cell 61 24930734
2019 SUMO1 SUMOylates and SENP3 deSUMOylates NLRP3 to orchestrate the inflammasome activation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 59 31914638
2024 METTL16-SENP3-LTF axis confers ferroptosis resistance and facilitates tumorigenesis in hepatocellular carcinoma. Journal of hematology & oncology 58 39218945
2008 Arf-induced turnover of the nucleolar nucleophosmin-associated SUMO-2/3 protease Senp3. Cell cycle (Georgetown, Tex.) 58 18948745
2021 Redox-sensitive enzyme SENP3 mediates vascular remodeling via de-SUMOylation of β-catenin and regulation of its stability. EBioMedicine 56 34000626
2014 De-SUMOylation of FOXC2 by SENP3 promotes the epithelial-mesenchymal transition in gastric cancer cells. Oncotarget 56 25216525
2020 Exosome-transmitted long non-coding RNA SENP3-EIF4A1 suppresses the progression of hepatocellular carcinoma. Aging 53 32602848
2002 Cell-cycle-dependent localisation of Ulp1, a Schizosaccharomyces pombe Pmt3 (SUMO)-specific protease. Journal of cell science 51 11884512
2018 DeSUMOylation of MKK7 kinase by the SUMO2/3 protease SENP3 potentiates lipopolysaccharide-induced inflammatory signaling in macrophages. The Journal of biological chemistry 44 29352108
2004 Drosophila Ulp1, a nuclear pore-associated SUMO protease, prevents accumulation of cytoplasmic SUMO conjugates. The Journal of biological chemistry 44 15294908
2022 The SUMO protease SENP3 regulates mitochondrial autophagy mediated by Fis1. EMBO reports 42 34994490
2005 The 2 microm plasmid causes cell death in Saccharomyces cerevisiae with a mutation in Ulp1 protease. Molecular and cellular biology 39 15870298
2014 SUMO as a solubility tag and in vivo cleavage of SUMO fusion proteins with Ulp1. Methods in molecular biology (Clifton, N.J.) 38 24943315
2012 The biphasic redox sensing of SENP3 accounts for the HIF-1 transcriptional activity shift by oxidative stress. Acta pharmacologica Sinica 36 22684029
2020 SENP3 Suppresses Osteoclastogenesis by De-conjugating SUMO2/3 from IRF8 in Bone Marrow-Derived Monocytes. Cell reports 35 32049023
2010 A novel mechanism for SUMO system control: regulated Ulp1 nucleolar sequestration. Molecular and cellular biology 35 20647537
2013 Overexpression of SENP3 in oral squamous cell carcinoma and its association with differentiation. Oncology reports 33 23467634
2004 A new Saccharomyces cerevisiae strain with a mutant Smt3-deconjugating Ulp1 protein is affected in DNA replication and requires Srs2 and homologous recombination for its viability. Molecular and cellular biology 33 15169880
2018 Mitotic Phosphorylation of SENP3 Regulates DeSUMOylation of Chromosome-Associated Proteins and Chromosome Stability. Cancer research 30 29438989
2015 SENP3 regulates the global protein turnover and the Sp1 level via antagonizing SUMO2/3-targeted ubiquitination and degradation. Protein & cell 30 26511642
2023 SENP3 facilitates M1 macrophage polarization via the HIF-1α/PKM2 axis in lipopolysaccharide-induced acute lung injury. Innate immunity 29 37016838
2018 Rpp1 Encodes a ULP1-NBS-LRR Protein That Controls Immunity to Phakopsora pachyrhizi in Soybean. Molecular plant-microbe interactions : MPMI 29 30303765
2013 Regulation of Toll signaling and inflammation by β-arrestin and the SUMO protease Ulp1. Genetics 29 24077307
2011 Sumo-dependent substrate targeting of the SUMO protease Ulp1. BMC biology 29 22034919
2014 mTOR signaling regulates nucleolar targeting of the SUMO-specific isopeptidase SENP3. Molecular and cellular biology 28 25288641
2020 The Critical Roles of the SUMO-Specific Protease SENP3 in Human Diseases and Clinical Implications. Frontiers in physiology 27 33192553
2014 SSP3 is a novel Plasmodium yoelii sporozoite surface protein with a role in gliding motility. Infection and immunity 27 25156733
2019 Protective role of the deSUMOylating enzyme SENP3 in myocardial ischemia-reperfusion injury. PloS one 26 30973885
2020 SENP3 in monocytes/macrophages up-regulates tissue factor and mediates lipopolysaccharide-induced acute lung injury by enhancing JNK phosphorylation. Journal of cellular and molecular medicine 22 32237051
2019 Regulation of SETD7 Methyltransferase by SENP3 Is Crucial for Sarcomere Organization and Cachexia. Cell reports 22 31141694
2020 P53 suppresses SENP3 phosphorylation to mediate G2 checkpoint. Cell discovery 21 32351703
2019 Nuclear Nrf2 Activity in Laryngeal Carcinoma is Regulated by SENP3 After Cisplatin-Induced Reactive Oxygen Species Stress. Journal of Cancer 21 31293646
2012 An upregulation of SENP3 after spinal cord injury: implications for neuronal apoptosis. Neurochemical research 20 23054070
2011 Desumoylation of the endoplasmic reticulum membrane VAP family protein Scs2 by Ulp1 and SUMO regulation of the inositol synthesis pathway. Molecular and cellular biology 20 22025676
2011 The nucleolar SUMO-specific protease SMT3IP1/SENP3 attenuates Mdm2-mediated p53 ubiquitination and degradation. Biochemical and biophysical research communications 18 21316347
2022 Iron chelation promotes mitophagy through SENP3-mediated deSUMOylation of FIS1. Autophagy 17 35275026
2021 Long Non-coding RNA SENP3-EIF4A1 Functions as a Sponge of miR-195-5p to Drive Triple-Negative Breast Cancer Progress by Overexpressing CCNE1. Frontiers in cell and developmental biology 17 33791304
2021 SENP3-Mediated PPARγ2 DeSUMOylation in BM-MSCs Potentiates Glucocorticoid-Induced Osteoporosis by Promoting Adipogenesis and Weakening Osteogenesis. Frontiers in cell and developmental biology 17 34249943
2015 The SUMO Protease SENP3 Orchestrates G2-M Transition and Spindle Assembly in Mouse Oocytes. Scientific reports 17 26493771
2025 SENP3 inhibition suppresses hepatocellular carcinoma progression and improves the efficacy of anti-PD-1 immunotherapy. Cell death and differentiation 16 39755756
2023 SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury. iScience 16 37332598
2018 SENP3 protects H9C2 cells from apoptosis triggered by H/R via STAT3 pathway. European review for medical and pharmacological sciences 16 29771430
2017 Inhibition of SENP3 by URB597 ameliorates neurovascular unit dysfunction in rats with chronic cerebral hypoperfusion. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 16 28501776
2016 Dynamic regulation of HIF1Α stability by SUMO2/3 and SENP3 in the human placenta. Placenta 16 27016777
2015 Inhibition of SENP3 by lentivirus induces suppression of apoptosis in experimental subarachnoid hemorrhage in rats. Brain research 16 26151898
2017 Increased SUMO-2/3-ylation mediated by SENP3 degradation is protective against cadmium-induced caspase 3-dependent cytotoxicity. The Journal of toxicological sciences 15 28747609
2008 SMT3IP1, a nucleolar SUMO-specific protease, deconjugates SUMO-2 from nucleolar and cytoplasmic nucleophosmin. Biochemical and biophysical research communications 15 18639523
2016 A Novel Strategy for the Preparation of Codon-Optimized Truncated Ulp1 and its Simplified Application to Cleavage the SUMO Fusion Protein. The protein journal 14 26960810
2023 Brucella effectors NyxA and NyxB target SENP3 to modulate the subcellular localisation of nucleolar proteins. Nature communications 13 36609656
2022 EAPB0503, an Imidazoquinoxaline Derivative Modulates SENP3/ARF Mediated SUMOylation, and Induces NPM1c Degradation in NPM1 Mutant AML. International journal of molecular sciences 13 35408798
2019 SENP3-mediated host defense response contains HBV replication and restores protein synthesis. PloS one 13 30640896
2018 Comparative study of the insoluble and soluble Ulp1 protease constructs as Carrier free and dependent protein immobilizates. Journal of bioscience and bioengineering 13 30001877
2017 SENP3 grants tight junction integrity and cytoskeleton architecture in mouse Sertoli cells. Oncotarget 13 28938568
2017 Flightless-I governs cell fate by recruiting the SUMO isopeptidase SENP3 to distinct HOX genes. Epigenetics & chromatin 12 28344658
2024 SENP3-FIS1 axis promotes mitophagy and cell survival under hypoxia. Cell death & disease 11 39638786
2022 SENP3 affects the expression of PYCR1 to promote bladder cancer proliferation and EMT transformation by deSUMOylation of STAT3. Aging 11 36227136
2021 SENP3 Promotes an Mff-Primed Bcl-x -Drp1 Interaction Involved in Cell Death Following Ischemia. Frontiers in cell and developmental biology 11 34722538
2019 A novel approach for production of an active N-terminally truncated Ulp1 (SUMO protease 1) catalytic domain from Escherichia coli inclusion bodies. Protein expression and purification 11 31586598
2025 SENP3 Drives Abdominal Aortic Aneurysm Development by Regulating Ferroptosis via De-SUMOylation of CTH. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10 40019399
2023 SENP3 and USP7 regulate Polycomb-rixosome interactions and silencing functions. Cell reports 10 37014752
2023 Myeloid SENP3 deficiency protects mice from diet and age-induced obesity via regulation of YAP1 SUMOylation. Cellular and molecular life sciences : CMLS 10 38070059
2018 Bioprocess optimization for the overproduction of catalytic domain of ubiquitin-like protease 1 (Ulp1) from S. cerevisiae in E. coli fed-batch culture. Enzyme and microbial technology 10 30396406
2025 SERPINH1 secretion by cancer-associated fibroblasts promotes hepatocellular carcinoma malignancy through SENP3-mediated SP1/SQLE pathway. International immunopharmacology 9 39946769
2022 Mitotic SENP3 activation couples with cGAS signaling in tumor cells to stimulate anti-tumor immunity. Cell death & disease 9 35869062
2020 A simple and rapid protein purification method based on cell-surface display of SUMO-fused recombinant protein and Ulp1 protease. AMB Express 9 32266507
2020 High SENP3 Expression Promotes Cell Migration, Invasion, and Proliferation by Modulating DNA Methylation of E-Cadherin in Osteosarcoma. Technology in cancer research & treatment 9 33030103
2015 Expression and Cell Distribution of SENP3 in Brain Tissue After Traumatic Brain Injury in Mice: A Pilot Study. Cellular and molecular neurobiology 9 25772139
2014 Expression and cell distribution of SENP3 in the cerebral cortex after experimental subarachnoid hemorrhage in rats: a pilot study. Cellular and molecular neurobiology 9 25423917
2010 A SUMO-Groucho Q domain fusion protein: characterization and in vivo Ulp1-mediated cleavage. Protein expression and purification 9 20732424
2025 SENP3 induced HADHA deSUMOylation enhances intrahepatic cholangiocarcinoma chemotherapy sensitivity via fatty acid oxidation. Cancer letters 8 40320039
2024 SENP3 mediates deSUMOylation of SIX1 to promote prostate cancer proliferation and migration. Cellular & molecular biology letters 8 39623295
2023 Overexpression of SENP3 promotes PPAR-γ transcription through the increase of HIF-1α stability via SUMO2/3 and participates in molecular mechanisms of osteoporosis. Molecular and cellular endocrinology 8 37473957
2022 SENP3-mediated TIP60 deSUMOylation is required for DNA-PKcs activity and DNA damage repair. MedComm 8 35356800
2022 Profiling Substrate Specificity of the SUMO Protease Ulp1 by the YESS-PSSC System to Advance the Conserved Mechanism for Substrate Cleavage. International journal of molecular sciences 8 36293045
2022 SENP3 Aggravates Renal Tubular Epithelial Cell Apoptosis in Lipopolysaccharide-Induced Acute Kidney Injury via deSUMOylation of Drp1. Kidney diseases (Basel, Switzerland) 8 36466072
2019 Plasmodium berghei sporozoite specific genes- PbS10 and PbS23/SSP3 are required for the development of exo-erythrocytic forms. Molecular and biochemical parasitology 8 31251952
2018 SUMO targeting of a stress-tolerant Ulp1 SUMO protease. PloS one 8 29351565
2024 SENP3 attenuates foam cell formation by deSUMOylating NLRP3 in macrophages stimulated with ox-LDL. Cellular signalling 7 38331013
2021 SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A. Journal of cell science 7 34313310
2020 SENP3 regulates high glucose-induced endothelial dysfunction via ROS dependent signaling. Diabetes & vascular disease research 7 33231124

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