Affinage

ERH

Enhancer of rudimentary homolog · UniProt P84090

Length
104 aa
Mass
12.3 kDa
Annotated
2026-06-09
100 papers in source corpus 15 papers cited in narrative 15 extracted findings
Cross-family judge faithfulness: 5/5 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ERH is a small, highly conserved nuclear protein that functions as an obligate homodimer to serve as a scaffold presenting multiple distinct ligand-binding surfaces that recruit RNA-processing and chromatin-regulatory factors to maintain genome integrity (PMID:15937287, PMID:17444515). Crystal structures established that ERH adopts a novel alpha+beta fold and dimerizes through its beta-sheet regions to form a pseudo-beta-barrel interface (PMID:15937287, PMID:17444515). Through this architecture ERH engages partners using surfaces that are biochemically separable: it binds the N-terminus of DGCR8 in a 2:2 stoichiometry as a Microprocessor component, where the ERH dimer bridges two Microprocessor complexes to mediate 'cluster assistance' processing of suboptimal pri-miRNAs in polycistronic clusters such as pri-miR-451 helped by pri-miR-144 (PMID:33035348, PMID:32302541); it associates with the Sm spliceosome component SNRPD3 and is required for splicing of the mitotic kinesin CENP-E, with ERH loss causing CENP-E depletion, kinetochore-microtubule attachment defects, and chromosome misalignment (PMID:23236152, PMID:22704934); and it binds a CIZ1 fragment via a surface distinct from that used for DGCR8, forming a 2:2 heterotetramer through intermolecular antiparallel beta-strand pairing (PMID:36047590, PMID:24015320). The same scaffolding logic is conserved in fission yeast, where the Erh1 ortholog forms a 2:2 complex with the YTH RNA-binding protein Mmi1 that is specifically required for facultative heterochromatin assembly and gene silencing (PMID:30651569). ERH depletion broadly perturbs cell-cycle gene expression, and KRAS-mutant cancer cells are preferentially sensitive to its loss (PMID:23236152); in bladder cancer it promotes migration, invasion, and metastasis via the downstream target MYC (PMID:30866868).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 1996 Medium

    Establishing ERH as a discrete, ubiquitously expressed human gene conserved with Drosophila enhancer of rudimentary provided the molecular entry point for all subsequent mechanistic work.

    Evidence cDNA cloning, sequence analysis, and FISH chromosomal mapping

    PMID:8786099

    Open questions at the time
    • No biochemical function assigned
    • No interacting partners identified
  2. 2001 Medium

    The first functional partner assignment came from linking ERH to DCoH/PCD-dependent HNF1 transcription, raising the possibility of a transcriptional regulatory role.

    Evidence Yeast two-hybrid and GAL4/HNF1-responsive reporter assays in cell lines

    PMID:11688721

    Open questions at the time
    • Cell-type-specific repression mechanism unresolved
    • No structural basis for the DCoH interaction
    • Direct DNA binding not demonstrated
  3. 2005 High

    Determining the ERH fold and demonstrating homodimerization defined the structural platform that underlies its scaffolding function.

    Evidence Murine ERH X-ray crystallography at 2.1 A with analytical ultracentrifugation

    PMID:15937287

    Open questions at the time
    • No partner-bound structure
    • Functional consequence of putative Thr18/Ser24 phosphorylation untested
  4. 2007 High

    An independent high-resolution human ERH structure confirmed the dimeric pseudo-beta-barrel architecture and mapped a candidate interface cavity for partner binding.

    Evidence Human ERH X-ray crystallography at 1.55 A with in silico docking

    PMID:17444515

    Open questions at the time
    • Docking-predicted DCOH regulation not experimentally validated
    • No co-complex structure
  5. 2012 High

    Connecting ERH to SNRPD3 and CENP-E splicing established a concrete RNA-processing function with a mitotic readout, and tied ERH dependency to KRAS-mutant cancers.

    Evidence Reciprocal co-IP, RNAi, gene expression profiling, and chromosome congression/kinetochore imaging across cell lines

    PMID:22704934 PMID:23236152

    Open questions at the time
    • Mechanism by which ERH selects CENP-E pre-mRNA unknown
    • Direct role within the spliceosome not structurally defined
    • Basis of KRAS-mutant selective vulnerability unexplained
  6. 2013 Medium

    Mutagenesis showed ERH uses biochemically separable surfaces to recruit distinct partners to distinct nuclear compartments, formalizing the multi-surface scaffold model.

    Evidence Site-directed mutagenesis, GST pull-down, and fluorescence microscopy of PDIP46/SKAR and Ciz1 recruitment

    PMID:24015320

    Open questions at the time
    • Functional output of speckle vs replication-foci localization unresolved
    • No co-complex structures at this stage
  7. 2015 Medium

    Identifying Erh mRNA as a CLIP target of MSI1 placed ERH within a posttranscriptional regulatory circuit during spermatogenesis.

    Evidence CLIP-seq and transgenic Msi1 overexpression model in mouse spermatogonia

    PMID:25782991

    Open questions at the time
    • Functional consequence of MSI1-mediated ERH regulation not established
    • Single lab
  8. 2016 Medium

    Mapping nuclear import to the ERH N-terminus and identifying ribosomal-protein partners suggested ERH reaches the nucleus by piggybacking on a nucleic-acid-binding partner rather than via a canonical NLS.

    Evidence Yeast two-hybrid, truncation mutagenesis, and nuclear localization assays in Drosophila and human cells

    PMID:27830090

    Open questions at the time
    • Direct import partner not confirmed in cells
    • Y2H interactions lack orthogonal validation
  9. 2019 High

    The Erh1-Mmi1 co-crystal structure with structure-guided mutagenesis demonstrated that a 2:2 ERH-YTH complex is specifically required for heterochromatin assembly and gene silencing, separable from transcription termination.

    Evidence Co-crystal structure, structure-guided mutagenesis, ChIP, RNA-seq, and genetics in fission yeast

    PMID:30651569

    Open questions at the time
    • Conservation of an analogous chromatin-silencing role in mammals not shown
    • Downstream silencing machinery recruited by EMC not fully defined
  10. 2020 High

    Structural and biochemical work defined ERH as a Microprocessor component that uses its hydrophobic groove to bind the DGCR8 N-terminus and bridge two Microprocessor complexes, enabling 'cluster assistance' processing of suboptimal pri-miRNAs.

    Evidence Co-crystal structure with stoichiometry, ERH knockdown, pri-miRNA processing/reporter assays, Northern blot, and RNA-seq (two independent labs)

    PMID:32302541 PMID:33035348

    Open questions at the time
    • In vivo extent of cluster assistance across the miRNA repertoire not mapped
    • How ERH dimer geometry constrains hairpin spacing not fully resolved
  11. 2022 High

    The ERH-CIZ1 co-crystal structure showed CIZ1 binds via an intermolecular beta-strand on a surface distinct from the DGCR8 and Mmi1 sites, structurally confirming that the ERH dimer presents multiple non-overlapping ligand surfaces; separately, ERH was linked to EIF2alpha and the integrated stress response.

    Evidence X-ray crystallography, GST pull-down, mutagenesis (CIZ1); co-IP/MS, IF colocalization, docking, and RT-PCR (EIF2alpha) in bladder cancer cells

    PMID:35774124 PMID:36047590

    Open questions at the time
    • Cellular function of the ERH-CIZ1 complex at replication foci not mechanistically defined
    • EIF2alpha interaction is single-lab co-IP/MS with predicted rather than validated interface

Open questions

Synthesis pass · forward-looking unresolved questions
  • How a single small dimer coordinates its many partner surfaces in a single cell, and whether its splicing, miRNA, replication-foci, and stress-response roles operate independently or are co-regulated, remains unresolved.
  • No unified model integrating ERH's distinct partner complexes
  • Regulation of partner selection (e.g., by phosphorylation) untested
  • Mammalian counterpart of the yeast heterochromatin-silencing role unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 4 GO:0140110 transcription regulator activity 2
Localization
GO:0005634 nucleus 2 GO:0005654 nucleoplasm 1
Pathway
R-HSA-8953854 Metabolism of RNA 3 R-HSA-1640170 Cell Cycle 2 R-HSA-4839726 Chromatin organization 1
Partners
CIZ1DCOH/PCDDGCR8EIF2S1MMI1PDIP46/SKARRPS3SNRPD3
Complex memberships
ERH homodimerErh1-Mmi1 complex (EMC)Microprocessor (DROSHA/DGCR8)

Evidence

Reading pass · 15 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2005 Crystal structure of murine ERH determined at 2.1 Å resolution, revealing a novel alpha+beta fold comprising three alpha-helices and four beta-strands. ERH forms a homodimer through interactions between beta-sheet regions, confirmed by analytical ultracentrifugation. Conserved residues at the core and dimer interface suggest conservation of both monomer fold and dimer formation. Putative phosphorylation sites were identified at Thr18 and Ser24. X-ray crystallography (MAD phasing), analytical ultracentrifugation Protein science : a publication of the Protein Society High 15937287
2007 Human ERH (HEF2/ERH) crystal structure determined at 1.55 Å, confirming the novel alpha+beta fold with a four-strand antiparallel beta-sheet and three alpha-helices. The physiological dimer forms a pseudo-beta-barrel at the interface with a cavity channel. Docking analysis suggests ERH may regulate the oligomeric state of its partner DCOH/PCD. X-ray crystallography, in silico protein-protein docking Proteins High 17444515
2001 Xenopus ERH (XERH), which is identical in amino acid sequence to mammalian ERH, was identified as a binding partner of DCoH/PCD (dimerization cofactor of HNF1) by yeast two-hybrid assay. When fused to the GAL4 DNA-binding domain, XERH represses a GAL4-responsive reporter in HeLa cells (but not NIH3T3 cells), and inhibits DCoH/PCD coactivation of an HNF1-responsive reporter, establishing ERH as a cell-type-specific transcriptional repressor that interferes with HNF1-dependent gene regulation via DCoH/PCD. Yeast two-hybrid, GAL4-fusion transcriptional reporter assay, HNF1-responsive reporter assay Biological chemistry Medium 11688721
2012 ERH interacts with the spliceosome protein SNRPD3 (an Sm complex component) and is required for the mRNA splicing of the mitotic motor protein CENP-E. Loss of ERH leads to loss of CENP-E protein and chromosome congression defects. ERH depletion also broadly alters expression of cell cycle genes. KRAS-mutant cancer cells show greater sensitivity to ERH depletion than KRAS wild-type cells. Co-immunoprecipitation, RNAi knockdown, gene expression profiling, chromosome congression assay Proceedings of the National Academy of Sciences of the United States of America High 23236152
2012 ERH depletion causes severe chromosome misalignment and weakened kinetochore-microtubule attachment during mitosis. ERH depletion causes dissociation of CENP-E (a mitotic kinesin involved in stabilizing kinetochore-microtubule attachment) from kinetochores, establishing that ERH contributes to chromosome alignment at the metaphase plate by maintaining CENP-E at kinetochores. RNAi knockdown, immunofluorescence microscopy of mitotic chromosomes and kinetochores Biochemical and biophysical research communications Medium 22704934
2019 In fission yeast, the ERH ortholog Erh1 forms the Erh1-Mmi1 complex (EMC) with the YTH-family RNA-binding protein Mmi1 in a 2:2 stoichiometry, mediated by a conserved hydrophobic dimer interface. Co-crystal structure of the EMC was determined. Structure-guided mutation of Mmi1 Trp112, required for Erh1 binding, causes defects in facultative heterochromatin assembly and gene silencing while leaving Mmi1-mediated transcription termination intact, demonstrating that the ERH–YTH interaction is specifically required for gene silencing. Co-crystal structure, structure-guided mutagenesis, ChIP, RNA-seq, genetic analysis Nature communications High 30651569
2020 ERH is a component of the Microprocessor complex (DROSHA/DGCR8). Crystal structure and biochemical experiments reveal that ERH uses its hydrophobic groove to bind a conserved region in the N-terminus of DGCR8 in a 2:2 stoichiometry. Knockdown of ERH or deletion of the DGCR8 N-terminus reduces processing of suboptimal pri-miRNAs in polycistronic miRNA clusters. ERH increases processing of suboptimal pri-miR-451 in a manner dependent on its neighboring pri-miR-144, suggesting that the ERH dimer mediates 'cluster assistance' by helping load Microprocessor onto poor substrates. Co-crystal structure, biochemical binding assays, ERH knockdown, pri-miRNA processing assay, Northern blot Nucleic acids research High 33035348
2020 ERH copurifies with Microprocessor and can dimerize and interact with other proteins that dimerize. ERH is required for the 'cluster assistance' phenomenon in which a helper miRNA hairpin in a polycistronic cluster augments processing of an otherwise defective neighboring hairpin (e.g., miR-451 with miR-144 as helper). This effect requires Microprocessor recognition of the helper hairpin, physical linkage of the two hairpins, and ERH, suggesting ERH bridges two Microprocessor complexes. Biochemical copurification, miRNA processing reporter assays, ERH knockdown/complementation, RNA-seq Molecular cell High 32302541
2013 ERH is localized to nuclear speckles when coexpressed with PDIP46/SKAR in HeLa cells, and to replication foci when coexpressed with Ciz1. Mutagenesis identified specific ERH residues: H3A Q9A mutations diminish recruitment to nuclear speckles but not to replication foci; E37A T51A mutations impair replication foci recruitment while preserving nuclear speckle accumulation. These mutants show loss of interaction with PDIP46/SKAR and/or Ciz1 in GST pull-down assays. The beta-sheet surface of ERH (monomer form) mediates interaction with PDIP46/SKAR, while the loop between alpha1 and alpha2 helices mediates Ciz1 interaction. Site-directed mutagenesis, fluorescence microscopy, GST pull-down assay PloS one Medium 24015320
2016 Drosophila ERH nuclear localization requires the first 24 amino acids (which contain no canonical NLS). Three new ERH binding partners were identified by yeast two-hybrid: RPS3, RPL19, and DDIT4. RPS3 was identified in both human and Drosophila screens. The ERH sequences required for RPS3 and RPL19 interactions map to the same 24-amino-acid N-terminal region required for nuclear localization, suggesting ERH enters the nucleus through association with one of these DNA/RNA-binding partners. Yeast two-hybrid, deletion/truncation mutagenesis, nuclear localization assay Molecular biology international Medium 27830090
2015 RNA-binding protein Musashi-1 (MSI1) directly targets Erh mRNA in mouse spermatogonia, as identified through crosslinking and immunoprecipitation (CLIP) and validated in a transgenic Msi1 overexpression model, establishing Erh as a posttranscriptional target of MSI1 during early spermatogenesis. CLIP-seq, transgenic overexpression model, expression studies FASEB journal : official publication of the Federation of American Societies for Experimental Biology Medium 25782991
2019 ERH knockdown in bladder cancer T24 and 5637 cells significantly inhibits cell migration and invasion in vitro and metastasis in vivo (nude mouse tail vein assay). Gene expression profiling revealed MYC as an important downstream target of ERH, and functional experiments confirmed MYC as a functional target mediating ERH's effect on metastasis in T24 cells. shRNA knockdown, wound healing assay, transwell migration/invasion assay, nude mouse metastasis assay, gene expression profiling, functional rescue experiments BMC cancer Medium 30866868
2022 ERH interacts with EIF2α (encoded by EIF2S1) in human T24 bladder cancer cells, as demonstrated by co-immunoprecipitation/shotgun mass spectrometry and immunofluorescence colocalization, with binding sites predicted by ZDOCK docking. ERH overexpression upregulates mRNA levels of ATF4 and CHOP, placing ERH upstream of the EIF2α/ATF4/CHOP integrated stress response pathway. Co-immunoprecipitation, shotgun mass spectrometry, immunofluorescence colocalization, ZDOCK computational docking, RT-PCR Frontiers in oncology Medium 35774124
2022 Crystal structure of the human ERH–CIZ1 complex was determined, showing that a CIZ1 fragment upstream of its first zinc finger is sufficient for ERH binding (confirmed by GST pull-down). The ERH dimer binds two CIZ1 fragments in a 2:2 heterotetramer. CIZ1 forms intermolecular antiparallel beta-strands with ERH, and its binding surface on ERH is distinct from those of other known ERH ligands (DGCR8, Mmi1). Interface validated by mutagenesis and binding experiments. X-ray crystallography, GST pull-down, mutagenesis, binding assays The FEBS journal High 36047590
1996 Human ERH cDNA was cloned, encoding a 104 amino acid protein with 80% amino acid identity to Drosophila DROER (enhancer of rudimentary). The ERH gene was mapped to chromosomal band 7q34 by fluorescence in situ hybridization (FISH). ERH mRNA is expressed in all normal human tissues examined. cDNA cloning, sequence analysis, FISH chromosomal mapping Genomics Medium 8786099

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2019 Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism. Nature communications 566 31672964
2005 Comparative studies on the hypolipidemic and growth suppressive effects of oolong, black, pu-erh, and green tea leaves in rats. Journal of agricultural and food chemistry 123 15656692
2021 Pu-erh tea ameliorates obesity and modulates gut microbiota in high fat diet fed mice. Food research international (Ottawa, Ont.) 122 34053553
2011 Effects of enzymatic action on the formation of theabrownin during solid state fermentation of Pu-erh tea. Journal of the science of food and agriculture 109 21656777
2015 Pu-erh tea polysaccharides decrease blood sugar by inhibition of α-glucosidase activity in vitro and in mice. Food & function 83 25820466
2015 An Integrated Metagenomics/Metaproteomics Investigation of the Microbial Communities and Enzymes in Solid-state Fermentation of Pu-erh tea. Scientific reports 83 25974221
2004 Effects of pu-erh tea on oxidative damage and nitric oxide scavenging. Journal of agricultural and food chemistry 74 15612813
2010 Effects of theabrownin from pu-erh tea on the metabolism of serum lipids in rats: mechanism of action. Journal of food science 73 20722930
2021 Aged Ripe Pu-erh Tea Reduced Oxidative Stress-Mediated Inflammation in Dextran Sulfate Sodium-Induced Colitis Mice by Regulating Intestinal Microbes. Journal of agricultural and food chemistry 64 34460244
2014 Oolong, black and pu-erh tea suppresses adiposity in mice via activation of AMP-activated protein kinase. Food & function 63 25098399
2011 Pu-erh tea, green tea, and black tea suppresses hyperlipidemia, hyperleptinemia and fatty acid synthase through activating AMPK in rats fed a high-fructose diet. Food & function 63 22127373
2020 MicroRNA Clustering Assists Processing of Suboptimal MicroRNA Hairpins through the Action of the ERH Protein. Molecular cell 60 32302541
2012 Evolutionarily conserved protein ERH controls CENP-E mRNA splicing and is required for the survival of KRAS mutant cancer cells. Proceedings of the National Academy of Sciences of the United States of America 60 23236152
2010 Application of metabolomics in the analysis of manufacturing type of pu-erh tea and composition changes with different postfermentation year. Journal of agricultural and food chemistry 60 19916505
2021 Ripened Pu-erh Tea Extract Promotes Gut Microbiota Resilience against Dextran Sulfate Sodium Induced Colitis. Journal of agricultural and food chemistry 57 33570405
2016 Pu-erh tea extract ameliorates high-fat diet-induced nonalcoholic steatohepatitis and insulin resistance by modulating hepatic IL-6/STAT3 signaling in mice. Journal of gastroenterology 49 26794005
2006 Free radical scavenging effect of Pu-erh tea extracts and their protective effect on oxidative damage in human fibroblast cells. Journal of agricultural and food chemistry 46 17032009
2009 Pu-erh tea attenuates hyperlipogenesis and induces hepatoma cells growth arrest through activating AMP-activated protein kinase (AMPK) in human HepG2 cells. Journal of agricultural and food chemistry 44 19459711
2022 Pu-erh tea and theabrownin ameliorate metabolic syndrome in mice via potential microbiota-gut-liver-brain interactions. Food research international (Ottawa, Ont.) 41 36461373
2021 Investigation and dynamic profiling of oligopeptides, free amino acids and derivatives during Pu-erh tea fermentation by ultra-high performance liquid chromatography tandem mass spectrometry. Food chemistry 39 34601212
2013 The enigmatic ERH protein: its role in cell cycle, RNA splicing and cancer. Protein & cell 38 24078386
2001 ERH (enhancer of rudimentary homologue), a conserved factor identical between frog and human, is a transcriptional repressor. Biological chemistry 37 11688721
2019 Theabrownin from Pu-erh tea together with swinging exercise synergistically ameliorates obesity and insulin resistance in rats. European journal of nutrition 36 31273522
2012 Studies on the bioactivity of aqueous extract of pu-erh tea and its fractions: in vitro antioxidant activity and α-glycosidase inhibitory property, and their effect on postprandial hyperglycemia in diabetic mice. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 35 23211442
2022 Pu-erh tea increases the metabolite Cinnabarinic acid to improve circadian rhythm disorder-induced obesity. Food chemistry 34 35749873
2020 ERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8. Nucleic acids research 34 33035348
2019 A conserved dimer interface connects ERH and YTH family proteins to promote gene silencing. Nature communications 33 30651569
2017 Pu-erh Tea Extract Ameliorates Ovariectomy-Induced Osteoporosis in Rats and Suppresses Osteoclastogenesis In Vitro. Frontiers in pharmacology 32 28620304
2010 Pu-erh black tea supplementation decreases quinocetone-induced ROS generation and oxidative DNA damage in Balb/c mice. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 32 21112366
2019 Pu-erh Tea Regulates Fatty Acid Metabolism in Mice Under High-Fat Diet. Frontiers in pharmacology 31 30804786
2005 Crystal structure of an enhancer of rudimentary homolog (ERH) at 2.1 Angstroms resolution. Protein science : a publication of the Protein Society 31 15937287
2005 Pu-erh tea supplementation suppresses fatty acid synthase expression in the rat liver through downregulating Akt and JNK signalings as demonstrated in human hepatoma HepG2 cells. Oncology research 31 16925113
2019 Aqueous raw and ripe Pu-erh tea extracts alleviate obesity and alter cecal microbiota composition and function in diet-induced obese rats. Applied microbiology and biotechnology 30 30610284
2019 The ERH gene regulates migration and invasion in 5637 and T24 bladder cancer cells. BMC cancer 29 30866868
2014 Component analysis of Pu-erh and its anti-constipation effects. Molecular medicine reports 29 24604453
2013 Structure and dynamics of the bacterial communities in fermentation of the traditional Chinese post-fermented pu-erh tea revealed by 16S rRNA gene clone library. World journal of microbiology & biotechnology 29 23591759
2022 Pu-erh Tea Restored Circadian Rhythm Disruption by Regulating Tryptophan Metabolism. Journal of agricultural and food chemistry 28 35475616
2016 Pu-erh Tea Protects the Nervous System by Inhibiting the Expression of Metabotropic Glutamate Receptor 5. Molecular neurobiology 28 27578019
2015 RNA binding protein Musashi-1 directly targets Msi2 and Erh during early testis germ cell development and interacts with IPO5 upon translocation to the nucleus. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27 25782991
2012 Enhancer of rudimentary homolog (ERH) plays an essential role in the progression of mitosis by promoting mitotic chromosome alignment. Biochemical and biophysical research communications 26 22704934
2017 Physicochemical properties and cell-based bioactivity of Pu'erh tea polysaccharide conjugates. International journal of biological macromolecules 25 28366855
2015 Pu-erh tea down-regulates sterol regulatory element-binding protein and stearyol-CoA desaturase to reduce fat storage in Caenorhaditis elegans. PloS one 25 25659129
2011 Pu-Erh tea and GABA attenuates oxidative stress in kainic acid-induced status epilepticus. Journal of biomedical science 25 22014163
2011 Pu-erh tea inhibits tumor cell growth by down-regulating mutant p53. International journal of molecular sciences 25 22174618
2020 Hot-water extract of ripened Pu-erh tea attenuates DSS-induced colitis through modulation of the NF-κB and HIF-1α signaling pathways in mice. Food & function 24 32239008
2020 Optimizing the extraction of bioactive compounds from pu-erh tea (Camellia sinensis var. assamica) and evaluation of antioxidant, cytotoxic, antimicrobial, antihemolytic, and inhibition of α-amylase and α-glucosidase activities. Food research international (Ottawa, Ont.) 24 33233112
2017 Pu'erh tea extract-mediated protection against hepatosteatosis and insulin resistance in mice with diet-induced obesity is associated with the induction of de novo lipogenesis in visceral adipose tissue. Journal of gastroenterology 24 28364190
2013 Free radical scavenging and anti-oxidative activities of an ethanol-soluble pigment extract prepared from fermented Zijuan Pu-erh tea. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 24 23831194
2022 Multi-omics analysis of the metabolism of phenolic compounds in tea leaves by Aspergillus luchuensis during fermentation of pu-erh tea. Food research international (Ottawa, Ont.) 23 36461293
2024 Identification and molecular mechanism of novel hypoglycemic peptide in ripened pu-erh tea: Molecular docking, dynamic simulation, and cell experiments. Food research international (Ottawa, Ont.) 22 39232541
2013 Pu-erh tea suppresses diet-induced body fat accumulation in C57BL/6J mice by down-regulating SREBP-1c and related molecules. Bioscience, biotechnology, and biochemistry 22 23832364
2008 Enhancer of the rudimentary gene homologue (ERH) expression pattern in sporadic human breast cancer and normal breast tissue. BMC cancer 22 18500978
1995 The embryonic RNA helicase gene (ERH): a new member of the DEAD box family of RNA helicases. The Biochemical journal 22 8948440
2019 Ripe and Raw Pu-Erh Tea: LC-MS Profiling, Antioxidant Capacity and Enzyme Inhibition Activities of Aqueous and Hydro-Alcoholic Extracts. Molecules (Basel, Switzerland) 21 30699941
2012 Inhibition of advanced glycation end product formation by Pu-erh tea ameliorates progression of experimental diabetic nephropathy. Journal of agricultural and food chemistry 21 22482420
2022 Pu-erh tea alleviated colitis-mediated brain dysfunction by promoting butyric acid production. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 20 36592713
2011 Inhibition of the replication of hepatitis B virus in vitro by pu-erh tea extracts. Journal of agricultural and food chemistry 20 21870867
2007 A 1.55 A resolution X-ray crystal structure of HEF2/ERH and insights into its transcriptional and cell-cycle interaction networks. Proteins 20 17444515
1996 Cloning and mapping of a novel human cDNA homologous to DROER, the enhancer of the Drosophila melanogaster rudimentary gene. Genomics 20 8786099
2023 Theabrownin of raw and ripened pu-erh tea varies in the alleviation of HFD-induced obesity via the regulation of gut microbiota. European journal of nutrition 19 37024732
2022 ERH Gene and Its Role in Cancer Cells. Frontiers in oncology 19 35677162
2014 Pu-erh tea extract induces the degradation of FET family proteins involved in the pathogenesis of amyotrophic lateral sclerosis. BioMed research international 19 24804206
2017 Pu-erh Tea Water Extract Mediates Cell Cycle Arrest and Apoptosis in MDA-MB-231 Human Breast Cancer Cells. Frontiers in pharmacology 18 28428754
2011 Pu-erh black tea extract supplementation attenuates the oxidative DNA damage and oxidative stress in Sprague-Dawley rats with renal dysfunction induced by subchronic 3-methyl-2-quinoxalin benzenevinylketo-1,4-dioxide exposure. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 18 22079314
2024 Theabrownin from Pu-erh Tea Improves DSS-Induced Colitis via Restoring Gut Homeostasis and Inhibiting TLR2&4 Signaling Pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology 17 39029137
2022 Theabrownin isolated from Pu-erh tea regulates Bacteroidetes to improve metabolic syndrome of rats induced by high-fat, high-sugar and high-salt diet. Journal of the science of food and agriculture 17 35040129
2020 Correlation analysis between filamentous fungi and chemical compositions in a pu-erh type tea after a long-term storage. Food science & nutrition 17 32405406
2022 ERH Interacts With EIF2α and Regulates the EIF2α/ATF4/CHOP Pathway in Bladder Cancer Cells. Frontiers in oncology 15 35774124
2017 Preventive effect of Silibinin in combination with Pu-erh tea extract on non-alcoholic fatty liver disease in ob/ob mice. Food & function 15 28164196
2012 Pu-erh tea reduces nitric oxide levels in rats by inhibiting inducible nitric oxide synthase expression through toll-like receptor 4. International journal of molecular sciences 15 22837686
2023 Theabrownin Isolated from Pu-Erh Tea Enhances the Innate Immune and Anti-Inflammatory Effects of RAW264.7 Macrophages via the TLR2/4-Mediated Signaling Pathway. Foods (Basel, Switzerland) 14 37048289
2021 Paenibacillus puerhi sp. nov., isolated from the rhizosphere soil of Pu-erh tea plants (Camellia sinensis var. assamica). Archives of microbiology 14 33386866
2019 Isolation, analysis and in vitro assessment of CYP3A4 inhibition by methylxanthines extracted from Pu-erh and Bancha tea leaves. Scientific reports 14 31558747
2013 Identification of amino acid residues of ERH required for its recruitment to nuclear speckles and replication foci in HeLa cells. PloS one 14 24015320
2013 Fermented Pu-erh tea increases in vitro anticancer activities in HT-29 cells and has antiangiogenetic effects on HUVECs. Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer 14 24579782
2024 Metagenomics-based gene exploration and biochemical characterization of novel glucoamylases and α-amylases in Daqu and Pu-erh tea microorganisms. International journal of biological macromolecules 13 39069062
2011 Antibacterial property and mechanism of a novel Pu-erh tea nanofibrous membrane. Applied microbiology and biotechnology 13 21858494
2023 The Characteristic Aroma Compounds of GABA Sun-Dried Green Tea and Raw Pu-Erh Tea Determined by Headspace Solid-Phase Microextraction Gas Chromatography-Mass Spectrometry and Relative Odor Activity Value. Foods (Basel, Switzerland) 12 38137315
2022 Mellow and Thick Taste of Pu-Erh Ripe Tea Based on Chemical Properties by Sensory-Directed Flavor Analysis. Foods (Basel, Switzerland) 12 35954052
2012 Effects of aqueous extracts of raw pu-erh tea and ripened pu-erh tea on proliferation and differentiation of 3T3-L1 preadipocytes. Phytotherapy research : PTR 12 23027678
2019 Guanine deaminase provides evidence of the increased caffeine content during the piling process of pu'erh tea. RSC advances 10 35540571
2013 Pu-erh tea hot-water extract activates Akt and induces insulin-independent glucose transport in rat skeletal muscle. Journal of medicinal food 10 23437791
2010 Protective effect of a new amide compound from Pu-erh tea on human micro-vascular endothelial cell against cytotoxicity induced by hydrogen peroxide. Fitoterapia 10 20970486
2018 Pu-erh Tea Ameliorates Atherosclerosis Associated with Promoting Macrophage Apoptosis by Reducing NF-κB Activation in ApoE Knockout Mice. Oxidative medicine and cellular longevity 9 30210650
2016 Pu-erh Tea Extract Attenuates Nicotine-Induced Foam Cell Formation in Primary Cultured Monocytes: An in Vitro Mechanistic Study. Journal of agricultural and food chemistry 9 27001463
2020 ERH proteins: connecting RNA processing to tumorigenesis? Current genetics 8 32144435
2016 Drosophila Enhancer of Rudimentary Homolog, ERH, Is a Binding Partner of RPS3, RPL19, and DDIT4, Suggesting a Mechanism for the Nuclear Localization of ERH. Molecular biology international 8 27830090
2024 Pu-erh tea theabrownin improves the ovarian function and gut microbiota in laying hens. Poultry science 7 38723460
2021 Proteogenomics Study of Blastobotrys adeninivorans TMCC 70007-A Dominant Yeast in the Fermentation Process of Pu-erh Tea. Journal of proteome research 7 34008989
2024 Theabrownin from Pu-erh tea attenuated high-fat diet-induced metabolic syndrome in rat by regulating microRNA and affecting gut microbiota. International journal of biological macromolecules 6 39638201
2023 Thirty Years with ERH: An mRNA Splicing and Mitosis Factor Only or Rather a Novel Genome Integrity Protector? Cells 6 37887293
2021 Apoptotic Effects of Anthocyanins from Vitis coignetiae Pulliat Are Enhanced by Augmented Enhancer of the Rudimentary Homolog (ERH) in Human Gastric Carcinoma MKN28 Cells. International journal of molecular sciences 6 33809701
2005 Overproduction, purification, crystallization and preliminary X-ray diffraction studies of the human transcription repressor ERH. Acta crystallographica. Section F, Structural biology and crystallization communications 6 16511088
2024 High lead-tolerant mutant Bacillus tropicus AT31-1 from rhizosphere soil of Pu-erh and its remediation mechanism. Bioresource technology 5 39521187
2022 Molecular basis for the recognition of CIZ1 by ERH. The FEBS journal 5 36047590
2021 Massilia puerhi sp. nov., isolated from soil of Pu-erh tea cellar. International journal of systematic and evolutionary microbiology 5 34499597
2012 [Spectroscopic and structural characteristics of the main components of Theabrownin in Pu-erh tea]. Guang pu xue yu guang pu fen xi = Guang pu 5 22715783
1981 Defective packing of an unusual DNA in a virulent Erwinia phage, Erh 1. Progress in clinical and biological research 5 7330047
2023 Effects of Pu-erh and Dian Hong tea polyphenols on the gut-liver axis in mice. AMB Express 4 37266757
2022 ERH: a plug-and-play protein important for gene silencing and cell cycle progression. The FEBS journal 4 36334004

Missed literature

Know a paper Affinage missed for ERH? Flag it for the maintainers and the community.

No submissions yet.