Affinage

ERH

Enhancer of rudimentary homolog · UniProt P84090

Round 2 corrected
Length
104 aa
Mass
12.3 kDa
Annotated
2026-04-28
130 papers in source corpus 14 papers cited in narrative 14 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ERH is a small, evolutionarily conserved homodimeric nuclear protein with a unique alpha+beta fold that functions as a multi-interface scaffold linking pre-mRNA splicing, miRNA biogenesis, heterochromatin formation, and cell-cycle progression (PMID:15937287, PMID:17444515). Through its interaction with the spliceosome component SNRPD3, ERH promotes correct splicing of cell-cycle transcripts including CENP-E, and its depletion causes chromosome misalignment due to loss of CENP-E from kinetochores (PMID:23236152, PMID:22704934). ERH binds the N-terminus of DGCR8 in a 2:2 stoichiometry to enable Microprocessor-mediated 'cluster assistance,' whereby suboptimal pri-miRNA hairpins within polycistronic clusters are efficiently processed (PMID:33035348, PMID:32302541). The ERH dimer engages distinct binding surfaces for different partners—DGCR8, CIZ1, Mmi1 (in fission yeast)—each validated by co-crystal structures and mutagenesis, establishing ERH as a versatile protein-protein interaction hub that uses separate interfaces to coordinate diverse nuclear RNA-processing and chromatin-regulatory functions (PMID:36047590, PMID:30651569, PMID:24015320).

Mechanistic history

Synthesis pass · year-by-year structured walk · 7 steps
  1. 2001 Medium

    The initial functional context of ERH was established when it was identified as a DCoH/PCD-interacting protein that acts as a cell-type-specific transcriptional repressor, linking this conserved small protein to HNF1-dependent gene regulation.

    Evidence Yeast two-hybrid and GAL4-reporter repression assays in HeLa and NIH3T3 cells

    PMID:11688721

    Open questions at the time
    • Interaction with DCoH/PCD not confirmed by reciprocal Co-IP or structural methods
    • Mechanism of cell-type specificity of repression unexplored
    • Endogenous target genes of ERH-DCoH/PCD complex not identified
  2. 2005 High

    Determination of the ERH crystal structure revealed a novel alpha+beta fold forming a homodimer through beta-sheet interactions, providing the first structural framework for understanding how ERH engages binding partners.

    Evidence X-ray crystallography (MAD phasing, 2.1 Å) with analytical ultracentrifugation confirming solution dimer

    PMID:15937287 PMID:17444515

    Open questions at the time
    • No partner-bound co-crystal structures yet available
    • Functional significance of the conserved flexible loop (residues 44–53) unresolved
    • Putative phosphorylation sites (Thr18, Ser24) not experimentally validated
  3. 2012 High

    ERH was shown to be required for mitotic chromosome alignment through its interaction with the spliceosome component SNRPD3 and its role in maintaining CENP-E mRNA splicing, establishing a direct mechanistic link between ERH-dependent splicing and cell-cycle progression.

    Evidence Co-immunoprecipitation, siRNA knockdown, live-cell imaging, gene expression profiling, and cell viability assays in human cancer cell lines

    PMID:22704934 PMID:23236152

    Open questions at the time
    • Whether ERH directly contacts pre-mRNA or acts solely via SNRPD3 is unclear
    • Full spectrum of ERH-dependent splicing targets not delineated
    • Structural basis of ERH–SNRPD3 interaction not determined
  4. 2013 High

    Mutagenesis revealed that ERH uses distinct surface patches to interact with different partners (PDIP46/SKAR for nuclear speckle recruitment versus CIZ1 for replication foci recruitment), establishing the multi-interface scaffold concept.

    Evidence Site-directed mutagenesis, immunofluorescence colocalization, and GST pull-down in HeLa cells

    PMID:24015320

    Open questions at the time
    • Functional consequence of ERH at replication foci not determined
    • Whether monomer-only interaction with PDIP46/SKAR reflects a dimer-disruption mechanism is untested
    • No co-crystal structures with these partners at this time
  5. 2019 High

    The co-crystal structure of fission yeast Erh1–Mmi1 (EMC) in a 2:2 complex demonstrated that ERH orthologs use a conserved hydrophobic interface to recruit RNA-binding proteins for facultative heterochromatin assembly and gametogenic gene silencing, extending ERH function to chromatin regulation.

    Evidence X-ray co-crystallography, structure-guided Mmi1 W112 mutagenesis, ChIP, gene expression assays in S. pombe

    PMID:30651569

    Open questions at the time
    • Whether mammalian ERH participates in analogous heterochromatin silencing is unknown
    • How EMC is recruited to specific genomic loci remains unclear
    • Relationship between EMC-mediated silencing and the RNAi pathway not fully resolved
  6. 2020 High

    Two studies converged to show that ERH binds DGCR8 in a 2:2 stoichiometry and is essential for Microprocessor-mediated 'cluster assistance,' whereby a high-affinity pri-miRNA hairpin facilitates processing of a neighboring suboptimal hairpin, revealing a new role for ERH in miRNA biogenesis.

    Evidence Crystal structure of ERH–DGCR8 N-terminus complex, siRNA knockdown, pri-miRNA processing assays, biochemical copurification, genetic dissection with hairpin variants

    PMID:32302541 PMID:33035348

    Open questions at the time
    • In vivo global impact on miRNA maturation profiles upon ERH loss not comprehensively mapped
    • Whether ERH-mediated Microprocessor dimerization occurs co-transcriptionally in chromatin context is unresolved
    • Structural mechanism of how ERH bridges two Microprocessor units at polycistronic loci not fully elucidated
  7. 2022 High

    The ERH–CIZ1 co-crystal structure revealed a distinct ligand-binding mode (intermolecular antiparallel beta-strands) on a surface separate from DGCR8 and Mmi1 binding sites, establishing that ERH uses at least three non-overlapping interfaces to engage different partners.

    Evidence X-ray crystallography, GST pull-down, mutagenesis, and binding assays

    PMID:36047590

    Open questions at the time
    • Functional role of ERH–CIZ1 complex at replication foci still uncharacterized
    • Whether simultaneous multi-partner engagement occurs in cells is unknown
    • No structure of ERH–SNRPD3 complex available to complete the interface map

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structural basis of ERH–SNRPD3 interaction and its role in splicing regulation, whether mammalian ERH participates in heterochromatin formation analogous to fission yeast EMC, and the full scope of ERH-dependent miRNA and mRNA targets that drive its essentiality in cell-cycle progression.
  • No ERH–SNRPD3 co-crystal structure
  • Comprehensive transcriptome-wide identification of ERH-dependent splicing events lacking
  • In vivo physiological role of ERH in mammalian development not determined using genetic models

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 5 GO:0005198 structural molecule activity 3 GO:0140110 transcription regulator activity 1
Localization
GO:0005634 nucleus 2 GO:0005654 nucleoplasm 1
Pathway
R-HSA-8953854 Metabolism of RNA 3 R-HSA-1640170 Cell Cycle 2 R-HSA-4839726 Chromatin organization 1
Partners
CIZ1DGCR8MMI1PCDPDIP46SNRPD3
Complex memberships
Erh1-Mmi1 complex (EMC; fission yeast)Microprocessor complex (via DGCR8)

Evidence

Reading pass · 14 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 Xenopus ERH (XERH), a 104 amino acid protein identical to its mammalian homologs, was identified as an interaction partner of DCoH/PCD (dimerization cofactor of HNF1) by yeast two-hybrid assay. When fused to the GAL4 DNA-binding domain, XERH represses a GAL4-responsive reporter in HeLa cells (but not NIH3T3 cells), and inhibits DCoH/PCD co-activation of an HNF1-responsive reporter, establishing ERH as a cell-type-specific transcriptional repressor that interferes with HNF1-dependent gene regulation via DCoH/PCD. Yeast two-hybrid, GAL4-reporter transcriptional repression assay in HeLa and NIH3T3 cells Biological chemistry Medium 11688721
2005 The crystal structure of murine ERH was determined at 2.1 Å resolution by MAD phasing, revealing a novel alpha+beta fold (three alpha-helices and four beta-strands) that forms a homodimer through beta-sheet interactions. Analytical ultracentrifugation confirmed dimer formation in solution. Highly conserved residues at the dimer interface and a conserved flexible loop (residues 44–53) were identified as likely functionally important features. Putative phosphorylation sites at Thr18 and Ser24 were noted. X-ray crystallography (MAD), analytical ultracentrifugation Protein science High 15937287
2007 The crystal structure of human HEF2/ERH was determined at 1.55 Å resolution, confirming the novel alpha+beta fold and a pseudo-beta-barrel at the dimer interface with a cavity channel. In silico docking to the partner protein DCOH/PCD suggested that ERH may regulate the oligomeric state of DCOH/PCD. In silico protein-interaction mapping indicated interactions with transcription factors and cell-cycle regulators. X-ray crystallography, computational protein docking Proteins High 17444515
2012 ERH depletion in human cells caused severe chromosome misalignment and weakened kinetochore-microtubule attachment during mitosis, with dissociation of the mitotic kinesin CENP-E from kinetochores. ERH was found to promote chromosome alignment at the metaphase plate by maintaining CENP-E localization at kinetochores. siRNA knockdown, immunofluorescence microscopy, live-cell imaging Biochemical and biophysical research communications Medium 22704934
2012 ERH interacts with the spliceosome protein SNRPD3 (an Sm complex component) and is required for the mRNA splicing of CENP-E, a mitotic motor protein. Loss of ERH leads to loss of CENP-E protein and chromosome congression defects. Gene expression profiling showed ERH is required for expression of multiple cell cycle genes. ERH depletion caused greater toxicity in KRAS-mutant cancer cells than in KRAS wild-type cells, identifying a non-oncogene addiction relationship. Co-immunoprecipitation, RNAi knockdown, gene expression profiling, cell viability assays Proceedings of the National Academy of Sciences of the United States of America High 23236152
2013 ERH is recruited to nuclear speckles when co-expressed with PDIP46/SKAR, and to replication foci when co-expressed with Ciz1, in HeLa cells. Mutagenesis identified specific amino acid residues critical for each localization: H3A/Q9A mutations (on the beta-sheet surface, monomer interface) reduce recruitment to nuclear speckles, while E37A/T51A mutations (near the loop between alpha1 and alpha2 helices) impair recruitment to replication foci. GST pull-down confirmed loss of interactions with PDIP46/SKAR and/or Ciz1 for respective mutants, showing only ERH monomers interact with PDIP46/SKAR while both monomers and dimers can interact with Ciz1. Site-directed mutagenesis, immunofluorescence, GST pull-down, co-transfection PloS one High 24015320
2015 RNA-binding protein Musashi-1 (MSI1) directly targets Erh mRNA in mouse spermatogonia, identified by RIP and overexpression studies using a transgenic Msi1 testis-specific overexpression model, establishing Erh as a translational regulatory target of MSI1 during early spermatogenesis. RNA immunoprecipitation (RIP), transgenic overexpression model, expression studies FASEB journal Medium 25782991
2016 Drosophila ERH binds RPS3, RPL19, and DDIT4 as identified by yeast two-hybrid screens using both human and Drosophila libraries. The first 24 amino acids of ERH are necessary and sufficient for nuclear localization, and the sequences required for interaction with RPS3 and RPL19 map to this same 24-amino-acid region, suggesting ERH may enter the nucleus by binding to ribosomal proteins such as RPS3 or RPL19 that have DNA-binding activity. Yeast two-hybrid, deletion mutagenesis, nuclear localization assays Molecular biology international Medium 27830090
2019 In fission yeast, the ERH ortholog Erh1 forms a homodimer that interacts with the YTH-family RNA-binding protein Mmi1 in a 2:2 stoichiometry (Erh1-Mmi1 complex, EMC) via a conserved hydrophobic molecular interface, as revealed by the co-crystal structure. Structure-guided mutation of Mmi1 Trp112, required for Erh1 binding, caused defects in facultative heterochromatin assembly and gametogenic gene silencing while leaving Mmi1-mediated transcription termination intact, delineating the specific contribution of EMC to gene silencing. X-ray co-crystallography, structure-guided mutagenesis, chromatin immunoprecipitation, gene expression assays Nature communications High 30651569
2019 ERH knockdown in human bladder urothelial carcinoma T24 and 5637 cells significantly inhibited cell migration and invasion in vitro, and reduced lung metastasis in nude mouse tail-vein transfer assays. Gene expression profiling identified MYC as a downstream target of ERH, and functional experiments confirmed that ERH regulates MYC expression to control metastatic behavior. shRNA knockdown, wound-healing assay, transwell migration/invasion assays, nude mouse metastasis model, gene expression profiling BMC cancer Medium 30866868
2020 ERH was identified as a component of the Microprocessor complex, binding to the N-terminus of DGCR8 via its hydrophobic groove in a 2:2 stoichiometry as revealed by crystal structure and biochemical experiments. Knockdown of ERH or deletion of the DGCR8 N-terminus reduced processing of suboptimal pri-miRNAs in polycistronic miRNA clusters, demonstrating that the ERH dimer mediates 'cluster assistance' by enabling Microprocessor to process a poor substrate when loaded via a high-affinity neighboring substrate. X-ray crystallography, biochemical binding assays, siRNA knockdown, pri-miRNA processing assays Nucleic acids research High 33035348
2020 ERH copurifies with Microprocessor and can dimerize and interact with other proteins that can dimerize, suggesting a model in which one Microprocessor recruits another Microprocessor to assist processing of suboptimal miRNA hairpins in clusters. The cluster assistance phenomenon for miR-451 requires Microprocessor recognition of a helper hairpin, physical linkage of the two hairpins, and ERH; it acts predominantly after helper-hairpin processing and can act reciprocally and non-co-transcriptionally. Biochemical copurification, miRNA processing assays, genetic dissection with hairpin variants Molecular cell High 32302541
2022 ERH interacts with EIF2α (encoded by EIF2S1) in human T24 bladder cancer cells, as confirmed by co-immunoprecipitation/mass spectrometry and immunofluorescence colocalization. The binding sites were predicted by ZDOCK docking. ERH overexpression upregulated mRNA levels of ATF4 and CHOP, downstream molecules in the EIF2α/ATF4/CHOP pathway, placing ERH as a regulator of ER stress signaling. Co-immunoprecipitation, shotgun mass spectrometry, immunofluorescence colocalization, ZDOCK docking, qPCR Frontiers in oncology Medium 35774124
2022 The crystal structure of ERH bound to a CIZ1 fragment was solved, revealing that the ERH dimer binds to two CIZ1 fragments in a 2:2 heterotetramer. CIZ1 forms intermolecular antiparallel beta-strands with ERH, and the CIZ1-binding surface on ERH is distinct from those of other known ERH-binding ligands (DGCR8 and Mmi1). Mutagenesis and binding experiments confirmed the ERH-CIZ1 interface, identifying a new ligand-binding mode for ERH. X-ray crystallography, GST pull-down, mutagenesis, binding assays The FEBS journal High 36047590

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 A human protein-protein interaction network: a resource for annotating the proteome. Cell 1704 16169070
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
2012 The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. Molecular cell 973 22681889
2005 Nucleolar proteome dynamics. Nature 934 15635413
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2009 A genome-wide RNAi screen identifies multiple synthetic lethal interactions with the Ras oncogene. Cell 843 19490893
2007 Large-scale mapping of human protein-protein interactions by mass spectrometry. Molecular systems biology 733 17353931
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2018 High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies. Molecular cell 580 29395067
2019 Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism. Nature communications 557 31672964
2017 Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. Science (New York, N.Y.) 533 28302793
2020 SARS-CoV-2 Disrupts Splicing, Translation, and Protein Trafficking to Suppress Host Defenses. Cell 449 33080218
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2010 Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science (New York, N.Y.) 421 20360068
2004 Dissection of the mammalian midbody proteome reveals conserved cytokinesis mechanisms. Science (New York, N.Y.) 417 15166316
2007 Functional specialization of beta-arrestin interactions revealed by proteomic analysis. Proceedings of the National Academy of Sciences of the United States of America 360 17620599
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2012 The cellular EJC interactome reveals higher-order mRNP structure and an EJC-SR protein nexus. Cell 272 23084401
2011 A directed protein interaction network for investigating intracellular signal transduction. Science signaling 258 21900206
2022 EWSR1-induced circNEIL3 promotes glioma progression and exosome-mediated macrophage immunosuppressive polarization via stabilizing IGF2BP3. Molecular cancer 257 35031058
2022 Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration. Cell 256 35063084
2018 Mapping the Genetic Landscape of Human Cells. Cell 225 30033366
2009 A genome-wide short hairpin RNA screening of jurkat T-cells for human proteins contributing to productive HIV-1 replication. The Journal of biological chemistry 211 19460752
2015 ∆F508 CFTR interactome remodelling promotes rescue of cystic fibrosis. Nature 209 26618866
2011 Next-generation sequencing to generate interactome datasets. Nature methods 200 21516116
2012 NOTCH1 nuclear interactome reveals key regulators of its transcriptional activity and oncogenic function. Molecular cell 174 23022380
2020 UFMylation maintains tumour suppressor p53 stability by antagonizing its ubiquitination. Nature cell biology 168 32807901
2019 H4K20me0 recognition by BRCA1-BARD1 directs homologous recombination to sister chromatids. Nature cell biology 162 30804502
2019 A protein-interaction network of interferon-stimulated genes extends the innate immune system landscape. Nature immunology 159 30833792
2005 Comparative studies on the hypolipidemic and growth suppressive effects of oolong, black, pu-erh, and green tea leaves in rats. Journal of agricultural and food chemistry 121 15656692
2021 Pu-erh tea ameliorates obesity and modulates gut microbiota in high fat diet fed mice. Food research international (Ottawa, Ont.) 120 34053553
2011 Effects of enzymatic action on the formation of theabrownin during solid state fermentation of Pu-erh tea. Journal of the science of food and agriculture 108 21656777
2015 Pu-erh tea polysaccharides decrease blood sugar by inhibition of α-glucosidase activity in vitro and in mice. Food & function 83 25820466
2015 An Integrated Metagenomics/Metaproteomics Investigation of the Microbial Communities and Enzymes in Solid-state Fermentation of Pu-erh tea. Scientific reports 82 25974221
2004 Effects of pu-erh tea on oxidative damage and nitric oxide scavenging. Journal of agricultural and food chemistry 74 15612813
2010 Effects of theabrownin from pu-erh tea on the metabolism of serum lipids in rats: mechanism of action. Journal of food science 73 20722930
2021 Aged Ripe Pu-erh Tea Reduced Oxidative Stress-Mediated Inflammation in Dextran Sulfate Sodium-Induced Colitis Mice by Regulating Intestinal Microbes. Journal of agricultural and food chemistry 64 34460244
2011 Pu-erh tea, green tea, and black tea suppresses hyperlipidemia, hyperleptinemia and fatty acid synthase through activating AMPK in rats fed a high-fructose diet. Food & function 63 22127373
2014 Oolong, black and pu-erh tea suppresses adiposity in mice via activation of AMP-activated protein kinase. Food & function 62 25098399
2010 Application of metabolomics in the analysis of manufacturing type of pu-erh tea and composition changes with different postfermentation year. Journal of agricultural and food chemistry 60 19916505
2020 MicroRNA Clustering Assists Processing of Suboptimal MicroRNA Hairpins through the Action of the ERH Protein. Molecular cell 58 32302541
2012 Evolutionarily conserved protein ERH controls CENP-E mRNA splicing and is required for the survival of KRAS mutant cancer cells. Proceedings of the National Academy of Sciences of the United States of America 58 23236152
2021 Ripened Pu-erh Tea Extract Promotes Gut Microbiota Resilience against Dextran Sulfate Sodium Induced Colitis. Journal of agricultural and food chemistry 55 33570405
2016 Pu-erh tea extract ameliorates high-fat diet-induced nonalcoholic steatohepatitis and insulin resistance by modulating hepatic IL-6/STAT3 signaling in mice. Journal of gastroenterology 49 26794005
2006 Free radical scavenging effect of Pu-erh tea extracts and their protective effect on oxidative damage in human fibroblast cells. Journal of agricultural and food chemistry 46 17032009
2009 Pu-erh tea attenuates hyperlipogenesis and induces hepatoma cells growth arrest through activating AMP-activated protein kinase (AMPK) in human HepG2 cells. Journal of agricultural and food chemistry 43 19459711
2022 Pu-erh tea and theabrownin ameliorate metabolic syndrome in mice via potential microbiota-gut-liver-brain interactions. Food research international (Ottawa, Ont.) 39 36461373
2021 Investigation and dynamic profiling of oligopeptides, free amino acids and derivatives during Pu-erh tea fermentation by ultra-high performance liquid chromatography tandem mass spectrometry. Food chemistry 37 34601212
2013 The enigmatic ERH protein: its role in cell cycle, RNA splicing and cancer. Protein & cell 37 24078386
2019 Theabrownin from Pu-erh tea together with swinging exercise synergistically ameliorates obesity and insulin resistance in rats. European journal of nutrition 36 31273522
2001 ERH (enhancer of rudimentary homologue), a conserved factor identical between frog and human, is a transcriptional repressor. Biological chemistry 36 11688721
2012 Studies on the bioactivity of aqueous extract of pu-erh tea and its fractions: in vitro antioxidant activity and α-glycosidase inhibitory property, and their effect on postprandial hyperglycemia in diabetic mice. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 35 23211442
2022 Pu-erh tea increases the metabolite Cinnabarinic acid to improve circadian rhythm disorder-induced obesity. Food chemistry 34 35749873
2019 A conserved dimer interface connects ERH and YTH family proteins to promote gene silencing. Nature communications 33 30651569
2017 Pu-erh Tea Extract Ameliorates Ovariectomy-Induced Osteoporosis in Rats and Suppresses Osteoclastogenesis In Vitro. Frontiers in pharmacology 32 28620304
2010 Pu-erh black tea supplementation decreases quinocetone-induced ROS generation and oxidative DNA damage in Balb/c mice. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 32 21112366
2020 ERH facilitates microRNA maturation through the interaction with the N-terminus of DGCR8. Nucleic acids research 31 33035348
2019 Pu-erh Tea Regulates Fatty Acid Metabolism in Mice Under High-Fat Diet. Frontiers in pharmacology 31 30804786
2005 Pu-erh tea supplementation suppresses fatty acid synthase expression in the rat liver through downregulating Akt and JNK signalings as demonstrated in human hepatoma HepG2 cells. Oncology research 31 16925113
2019 Aqueous raw and ripe Pu-erh tea extracts alleviate obesity and alter cecal microbiota composition and function in diet-induced obese rats. Applied microbiology and biotechnology 30 30610284
2005 Crystal structure of an enhancer of rudimentary homolog (ERH) at 2.1 Angstroms resolution. Protein science : a publication of the Protein Society 30 15937287
2014 Component analysis of Pu-erh and its anti-constipation effects. Molecular medicine reports 29 24604453
2013 Structure and dynamics of the bacterial communities in fermentation of the traditional Chinese post-fermented pu-erh tea revealed by 16S rRNA gene clone library. World journal of microbiology & biotechnology 29 23591759
2022 Pu-erh Tea Restored Circadian Rhythm Disruption by Regulating Tryptophan Metabolism. Journal of agricultural and food chemistry 28 35475616
2019 The ERH gene regulates migration and invasion in 5637 and T24 bladder cancer cells. BMC cancer 28 30866868
2016 Pu-erh Tea Protects the Nervous System by Inhibiting the Expression of Metabotropic Glutamate Receptor 5. Molecular neurobiology 28 27578019
2015 RNA binding protein Musashi-1 directly targets Msi2 and Erh during early testis germ cell development and interacts with IPO5 upon translocation to the nucleus. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27 25782991
2012 Enhancer of rudimentary homolog (ERH) plays an essential role in the progression of mitosis by promoting mitotic chromosome alignment. Biochemical and biophysical research communications 26 22704934
2017 Physicochemical properties and cell-based bioactivity of Pu'erh tea polysaccharide conjugates. International journal of biological macromolecules 25 28366855
2015 Pu-erh tea down-regulates sterol regulatory element-binding protein and stearyol-CoA desaturase to reduce fat storage in Caenorhaditis elegans. PloS one 25 25659129
2011 Pu-erh tea inhibits tumor cell growth by down-regulating mutant p53. International journal of molecular sciences 25 22174618
2020 Hot-water extract of ripened Pu-erh tea attenuates DSS-induced colitis through modulation of the NF-κB and HIF-1α signaling pathways in mice. Food & function 24 32239008
2020 Optimizing the extraction of bioactive compounds from pu-erh tea (Camellia sinensis var. assamica) and evaluation of antioxidant, cytotoxic, antimicrobial, antihemolytic, and inhibition of α-amylase and α-glucosidase activities. Food research international (Ottawa, Ont.) 24 33233112
2017 Pu'erh tea extract-mediated protection against hepatosteatosis and insulin resistance in mice with diet-induced obesity is associated with the induction of de novo lipogenesis in visceral adipose tissue. Journal of gastroenterology 24 28364190
2013 Free radical scavenging and anti-oxidative activities of an ethanol-soluble pigment extract prepared from fermented Zijuan Pu-erh tea. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 24 23831194
2011 Pu-Erh tea and GABA attenuates oxidative stress in kainic acid-induced status epilepticus. Journal of biomedical science 23 22014163
2013 Pu-erh tea suppresses diet-induced body fat accumulation in C57BL/6J mice by down-regulating SREBP-1c and related molecules. Bioscience, biotechnology, and biochemistry 22 23832364
2008 Enhancer of the rudimentary gene homologue (ERH) expression pattern in sporadic human breast cancer and normal breast tissue. BMC cancer 22 18500978
1995 The embryonic RNA helicase gene (ERH): a new member of the DEAD box family of RNA helicases. The Biochemical journal 22 8948440
2012 Inhibition of advanced glycation end product formation by Pu-erh tea ameliorates progression of experimental diabetic nephropathy. Journal of agricultural and food chemistry 21 22482420
2024 Identification and molecular mechanism of novel hypoglycemic peptide in ripened pu-erh tea: Molecular docking, dynamic simulation, and cell experiments. Food research international (Ottawa, Ont.) 20 39232541
2022 Multi-omics analysis of the metabolism of phenolic compounds in tea leaves by Aspergillus luchuensis during fermentation of pu-erh tea. Food research international (Ottawa, Ont.) 20 36461293
2011 Inhibition of the replication of hepatitis B virus in vitro by pu-erh tea extracts. Journal of agricultural and food chemistry 20 21870867
2007 A 1.55 A resolution X-ray crystal structure of HEF2/ERH and insights into its transcriptional and cell-cycle interaction networks. Proteins 20 17444515
1996 Cloning and mapping of a novel human cDNA homologous to DROER, the enhancer of the Drosophila melanogaster rudimentary gene. Genomics 20 8786099
2023 Theabrownin of raw and ripened pu-erh tea varies in the alleviation of HFD-induced obesity via the regulation of gut microbiota. European journal of nutrition 19 37024732
2019 Ripe and Raw Pu-Erh Tea: LC-MS Profiling, Antioxidant Capacity and Enzyme Inhibition Activities of Aqueous and Hydro-Alcoholic Extracts. Molecules (Basel, Switzerland) 19 30699941
2022 ERH Gene and Its Role in Cancer Cells. Frontiers in oncology 18 35677162
2017 Pu-erh Tea Water Extract Mediates Cell Cycle Arrest and Apoptosis in MDA-MB-231 Human Breast Cancer Cells. Frontiers in pharmacology 18 28428754
2014 Pu-erh tea extract induces the degradation of FET family proteins involved in the pathogenesis of amyotrophic lateral sclerosis. BioMed research international 18 24804206
2011 Pu-erh black tea extract supplementation attenuates the oxidative DNA damage and oxidative stress in Sprague-Dawley rats with renal dysfunction induced by subchronic 3-methyl-2-quinoxalin benzenevinylketo-1,4-dioxide exposure. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 18 22079314
2022 Theabrownin isolated from Pu-erh tea regulates Bacteroidetes to improve metabolic syndrome of rats induced by high-fat, high-sugar and high-salt diet. Journal of the science of food and agriculture 17 35040129
2022 Pu-erh tea alleviated colitis-mediated brain dysfunction by promoting butyric acid production. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 17 36592713
2020 Correlation analysis between filamentous fungi and chemical compositions in a pu-erh type tea after a long-term storage. Food science & nutrition 17 32405406
2024 Theabrownin from Pu-erh Tea Improves DSS-Induced Colitis via Restoring Gut Homeostasis and Inhibiting TLR2&4 Signaling Pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology 16 39029137
2022 ERH Interacts With EIF2α and Regulates the EIF2α/ATF4/CHOP Pathway in Bladder Cancer Cells. Frontiers in oncology 15 35774124
2017 Preventive effect of Silibinin in combination with Pu-erh tea extract on non-alcoholic fatty liver disease in ob/ob mice. Food & function 15 28164196
2012 Pu-erh tea reduces nitric oxide levels in rats by inhibiting inducible nitric oxide synthase expression through toll-like receptor 4. International journal of molecular sciences 15 22837686
2023 Theabrownin Isolated from Pu-Erh Tea Enhances the Innate Immune and Anti-Inflammatory Effects of RAW264.7 Macrophages via the TLR2/4-Mediated Signaling Pathway. Foods (Basel, Switzerland) 14 37048289
2021 Paenibacillus puerhi sp. nov., isolated from the rhizosphere soil of Pu-erh tea plants (Camellia sinensis var. assamica). Archives of microbiology 14 33386866
2019 Isolation, analysis and in vitro assessment of CYP3A4 inhibition by methylxanthines extracted from Pu-erh and Bancha tea leaves. Scientific reports 14 31558747
2013 Fermented Pu-erh tea increases in vitro anticancer activities in HT-29 cells and has antiangiogenetic effects on HUVECs. Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer 14 24579782
2013 Identification of amino acid residues of ERH required for its recruitment to nuclear speckles and replication foci in HeLa cells. PloS one 13 24015320
2011 Antibacterial property and mechanism of a novel Pu-erh tea nanofibrous membrane. Applied microbiology and biotechnology 13 21858494
2023 The Characteristic Aroma Compounds of GABA Sun-Dried Green Tea and Raw Pu-Erh Tea Determined by Headspace Solid-Phase Microextraction Gas Chromatography-Mass Spectrometry and Relative Odor Activity Value. Foods (Basel, Switzerland) 12 38137315
2012 Effects of aqueous extracts of raw pu-erh tea and ripened pu-erh tea on proliferation and differentiation of 3T3-L1 preadipocytes. Phytotherapy research : PTR 12 23027678
2024 Metagenomics-based gene exploration and biochemical characterization of novel glucoamylases and α-amylases in Daqu and Pu-erh tea microorganisms. International journal of biological macromolecules 11 39069062
2022 Mellow and Thick Taste of Pu-Erh Ripe Tea Based on Chemical Properties by Sensory-Directed Flavor Analysis. Foods (Basel, Switzerland) 11 35954052
2013 Pu-erh tea hot-water extract activates Akt and induces insulin-independent glucose transport in rat skeletal muscle. Journal of medicinal food 10 23437791
2010 Protective effect of a new amide compound from Pu-erh tea on human micro-vascular endothelial cell against cytotoxicity induced by hydrogen peroxide. Fitoterapia 10 20970486
2019 Guanine deaminase provides evidence of the increased caffeine content during the piling process of pu'erh tea. RSC advances 9 35540571
2018 Pu-erh Tea Ameliorates Atherosclerosis Associated with Promoting Macrophage Apoptosis by Reducing NF-κB Activation in ApoE Knockout Mice. Oxidative medicine and cellular longevity 9 30210650
2016 Pu-erh Tea Extract Attenuates Nicotine-Induced Foam Cell Formation in Primary Cultured Monocytes: An in Vitro Mechanistic Study. Journal of agricultural and food chemistry 9 27001463
2020 ERH proteins: connecting RNA processing to tumorigenesis? Current genetics 8 32144435
2016 Drosophila Enhancer of Rudimentary Homolog, ERH, Is a Binding Partner of RPS3, RPL19, and DDIT4, Suggesting a Mechanism for the Nuclear Localization of ERH. Molecular biology international 8 27830090
2024 Pu-erh tea theabrownin improves the ovarian function and gut microbiota in laying hens. Poultry science 7 38723460
2021 Proteogenomics Study of Blastobotrys adeninivorans TMCC 70007-A Dominant Yeast in the Fermentation Process of Pu-erh Tea. Journal of proteome research 7 34008989
2024 Theabrownin from Pu-erh tea attenuated high-fat diet-induced metabolic syndrome in rat by regulating microRNA and affecting gut microbiota. International journal of biological macromolecules 6 39638201
2021 Apoptotic Effects of Anthocyanins from Vitis coignetiae Pulliat Are Enhanced by Augmented Enhancer of the Rudimentary Homolog (ERH) in Human Gastric Carcinoma MKN28 Cells. International journal of molecular sciences 6 33809701
2005 Overproduction, purification, crystallization and preliminary X-ray diffraction studies of the human transcription repressor ERH. Acta crystallographica. Section F, Structural biology and crystallization communications 6 16511088
2024 High lead-tolerant mutant Bacillus tropicus AT31-1 from rhizosphere soil of Pu-erh and its remediation mechanism. Bioresource technology 5 39521187
2023 Thirty Years with ERH: An mRNA Splicing and Mitosis Factor Only or Rather a Novel Genome Integrity Protector? Cells 5 37887293
2022 Molecular basis for the recognition of CIZ1 by ERH. The FEBS journal 5 36047590
2021 Massilia puerhi sp. nov., isolated from soil of Pu-erh tea cellar. International journal of systematic and evolutionary microbiology 5 34499597
2012 [Spectroscopic and structural characteristics of the main components of Theabrownin in Pu-erh tea]. Guang pu xue yu guang pu fen xi = Guang pu 5 22715783
1981 Defective packing of an unusual DNA in a virulent Erwinia phage, Erh 1. Progress in clinical and biological research 5 7330047
2023 Effects of Pu-erh and Dian Hong tea polyphenols on the gut-liver axis in mice. AMB Express 4 37266757
2012 [The mechanisms of weight-cutting effect and bioactive components in Pu-erh tea]. Dong wu xue yan jiu = Zoological research 4 22855451