Affinage

DDX39A

ATP-dependent RNA helicase DDX39A · UniProt O00148

Length
427 aa
Mass
49.1 kDa
Annotated
2026-04-28
38 papers in source corpus 25 papers cited in narrative 24 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

DDX39A is a DEAD-box RNA helicase that functions as a central coordinator of mRNA nuclear export, alternative splicing, telomere maintenance, replication fork stability, and innate antiviral defense. It possesses RNA-dependent ATPase and bidirectional RNA unwinding activities (PMID:17548965) and assembles the AREX complex with CIP29 in its apo state, which remodels upon ATP binding into a TREX-like complex containing the THO subcomplex and ALYREF to drive NXF1-dependent export of specific mRNA subsets distinct from those controlled by its paralog DDX39B (PMID:20573985, PMID:31917363, PMID:38225262). Beyond mRNA export, DDX39A loads PHAX onto U snRNA for spliceosomal snRNA export (PMID:39011894), resolves RNA–DNA hybrids at stalled replication forks to facilitate DNA2-mediated resection and fork restart (PMID:39706186), directly binds conserved alphavirus genomic RNA structures to restrict replication in an interferon-independent manner (PMID:37949067), interacts with TRF2 and hTERT to regulate telomere length (PMID:21388492), and is itself regulated by RanBP2-mediated SUMO1 modification that attenuates RNA binding (PMID:32393512) and by Erk2 phosphorylation at Y132/Y138 that modulates telomere association and chromatin remodeling (PMID:39107495).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 2004 High

    Establishing DDX39A as a conserved mRNA export factor: URH49 interacted with export adaptor Aly and rescued loss of yeast Sub2p, demonstrating functional conservation in mRNA splicing and nuclear export.

    Evidence Yeast complementation assay and Co-IP with Aly

    PMID:15047853

    Open questions at the time
    • No identification of DDX39A-specific versus DDX39B-specific mRNA targets
    • Mechanism of DDX39A recruitment to mRNA not defined
  2. 2006 High

    Demonstrating DDX39A is essential for bulk mRNA export and identifying its protein interaction network: siRNA depletion caused nuclear poly(A)+ mRNA accumulation, while Co-IP/MS identified CIP29 as a direct partner that enhances DDX39A unwinding activity, alongside ALY and FUS/TLS.

    Evidence RNAi with FISH for poly(A)+ RNA; Co-IP, mass spectrometry, and RNA unwinding assays with CIP29

    PMID:16949219 PMID:17196963

    Open questions at the time
    • Whether CIP29 enhancement of helicase activity is direct or via complex assembly unclear
    • Specific mRNA targets not identified
  3. 2007 High

    Confirming DDX39A as a bona fide RNA helicase: recombinant DDX39A bound RNA, hydrolyzed NTPs in an RNA-dependent manner, and unwound dsRNA bidirectionally.

    Evidence In vitro reconstitution with purified recombinant protein: RNA-binding, NTPase, and unwinding assays

    PMID:17548965

    Open questions at the time
    • No structural basis for bidirectional unwinding
    • Substrate specificity determinants unknown
  4. 2010 High

    Resolving paralog specificity: DDX39A forms the AREX complex (distinct from the UAP56/TREX complex), controls different mRNA subsets, and its depletion causes specific mitotic defects including chromosome arm resolution failure and cytokinesis defects.

    Evidence RNAi knockdown with genome-wide mRNA profiling, Co-IP defining AREX composition, mitotic phenotype analysis

    PMID:20573985

    Open questions at the time
    • How AREX versus TREX achieve mRNA substrate discrimination unknown
    • Direct mechanism linking DDX39A mRNA targets to mitotic phenotype not established
  5. 2011 High

    Linking DDX39A to telomere biology and antiviral defense networks: DDX39A bound TRF2 via an FXLXP motif and associated with hTERT to regulate telomere length; separately, antiviral GTPase MxA was shown to directly bind and sequester DDX39A, and DDX39A was shown to shuttle between nucleus and cytoplasm independently of CRM1.

    Evidence Reciprocal Co-IP with domain mapping for TRF2; telomere FISH/TIF assays; in vitro binding with purified MxA; heterokaryon shuttling assay

    PMID:21388492 PMID:21799930 PMID:21859714

    Open questions at the time
    • How helicase activity contributes to telomere maintenance not resolved
    • Whether MxA sequestration of DDX39A is the primary antiviral mechanism in vivo unknown
  6. 2016 Medium

    Identifying DDX39A as a regulator of alternative splicing of specific oncogenic transcripts: combined DDX39A/B knockdown selectively downregulated the AR-V7 splice variant in prostate cancer cells.

    Evidence shRNA library screen validated by RNAi and RT-qPCR in multiple cell lines

    PMID:28025139

    Open questions at the time
    • Individual contribution of DDX39A versus DDX39B to AR-V7 splicing not deconvolved
    • Direct binding to AR pre-mRNA not shown
  7. 2020 High

    Defining post-translational regulation of DDX39A and ATP-dependent complex remodeling: RanBP2-mediated SUMO1 modification attenuated DDX39A RNA binding; viral infection reduced SUMOylation, increasing DDX39A retention of innate immune mRNAs in the nucleus. Separately, the apo-AREX complex was shown to remodel upon ATP addition into a TREX-like complex for NXF1-dependent export.

    Evidence SUMOylation assays with RanBP2, RNA immunoprecipitation, nuclear fractionation; ATP depletion/addition Co-IP complex assembly assays

    PMID:31917363 PMID:32393512

    Open questions at the time
    • Whether SUMO1 modification is the primary switch controlling DDX39A RNA binding during infection versus other modifications
    • Stoichiometry and structure of the AREX-to-TREX transition unknown
  8. 2023 High

    Establishing DDX39A as a direct cell-intrinsic antiviral effector: upon CHIKV infection, DDX39A relocated from nucleus to cytoplasm, directly bound the alphavirus 5'CSE RNA structure, and inhibited replication independently of interferon signaling.

    Evidence Genetic screen, RIP, biochemical binding assays mapping interaction to 5'CSE, IFN-independent antiviral assay

    PMID:37949067

    Open questions at the time
    • Whether DDX39A unwinds viral RNA structures or acts as a steric block not resolved
    • Breadth of antiviral activity beyond alphaviruses not fully mapped
  9. 2024 High

    Revealing DDX39A's role in replication fork stability and genome integrity: DDX39A resolves RNA–DNA hybrids at stalled forks via RAD51-dependent recruitment, facilitating DNA2-mediated resection and fork restart; its loss confers fork protection and chemoresistance in BRCA1/2-deficient cells.

    Evidence Two independent studies using Co-IP with RAD51, proximity ligation at forks, DNA fiber assay, DRIP-seq

    PMID:39706185 PMID:39706186

    Open questions at the time
    • Whether helicase activity or RNA binding alone resolves RF-RDs not fully dissected
    • Therapeutic implications of DDX39A loss in BRCA-deficient cancers not clinically validated
  10. 2024 High

    Expanding DDX39A's functions to U snRNA export and defining structural determinants of paralog specificity: DDX39A loads PHAX onto U snRNA in an ATP-dependent manner for snRNA export; structural and chimeric analyses revealed that C-terminal sequences of DDX39A are indispensable for AREX assembly and distinguish it from DDX39B/TREX.

    Evidence In vitro reconstitution of PHAX loading; crystal structures and chimeric mutant Co-IP/export assays; genome-wide splicing analysis with minigene validation

    PMID:38225262 PMID:38801080 PMID:39011894

    Open questions at the time
    • No high-resolution structure of the full AREX complex
    • How PHAX loading by DDX39A is coordinated with mRNA export function unclear
  11. 2024 High

    Connecting DDX39A to Erk2 signaling, telomere regulation, and chromatin remodeling: Erk2 phosphorylates DDX39A at Y132/Y138, weakening its interaction with Trf1 and releasing it from telomeres to allow ALT-mediated elongation; phosphorylated DDX39A also recruits Hat1 for H3K27 acetylation at differentiation genes.

    Evidence In vitro kinase assay, Ddx39 KO ESCs, ChIP, CO-FISH for ALT activity

    PMID:39107495

    Open questions at the time
    • Whether the Erk2-DDX39A-telomere axis operates in somatic cells beyond ESCs
    • Structural basis for phosphorylation-dependent dissociation from Trf1 unknown
  12. 2025 Medium

    Linking DDX39A to human disease: a pathogenic DDX39A-K137Q variant disrupted THOC1 interaction and TREX complex integrity, causing aberrant nuclear clumping, nuclear lamina disorganization, and increased cell death in patient-derived fibroblasts.

    Evidence Patient-derived fibroblast functional studies, Co-IP showing loss of THOC1 binding, structural modeling, nuclear morphology analysis

    PMID:40726340

    Open questions at the time
    • Clinical phenotype and inheritance pattern not fully characterized in the timeline
    • Whether K137Q affects AREX assembly independently of TREX not tested
    • Single patient study; independent replication needed

Open questions

Synthesis pass · forward-looking unresolved questions
  • Major open questions include: the high-resolution structure of the AREX complex and the molecular basis of mRNA substrate discrimination between DDX39A/AREX and DDX39B/TREX; how DDX39A's helicase activity is partitioned among its multiple functions (mRNA export, splicing, R-loop resolution, antiviral defense); and whether DDX39A mutations cause a defined Mendelian disorder.
  • No cryo-EM or crystal structure of the intact AREX complex
  • No systems-level model integrating DDX39A's multiple activities
  • In vivo validation of DDX39A as a therapeutic target in chemoresistant BRCA-deficient cancers lacking

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 7 GO:0140657 ATP-dependent activity 3 GO:0098772 molecular function regulator activity 2 GO:0140098 catalytic activity, acting on RNA 2
Localization
GO:0005654 nucleoplasm 5 GO:0005694 chromosome 2 GO:0005829 cytosol 1
Pathway
R-HSA-8953854 Metabolism of RNA 8 R-HSA-9609507 Protein localization 5 R-HSA-74160 Gene expression (Transcription) 4 R-HSA-168256 Immune System 2 R-HSA-1640170 Cell Cycle 1 R-HSA-73894 DNA Repair 1
Partners
ALYREFCIP29HTERTNXF1RAD51RANBP2THOC1TRF2
Complex memberships
AREX complexTREX-like complex (ATP-remodeled)

Evidence

Reading pass · 24 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2006 DDX39A (DDX39) is ubiquitylated and its stability is regulated via the ubiquitin-proteasome pathway; it tethers ALY (an mRNA export factor) in vivo; CIP29 was identified as a main DDX39-interacting protein by Co-IP and mass spectrometry, and CIP29 binds RNA on its own and enhances the RNA unwinding activity of DDX39; FUS/TLS was identified as a CIP29-interacting protein, placing DDX39 in a network with ALY, CIP29, and FUS/TLS in transcription, splicing, and RNA export. Co-immunoprecipitation, mass spectrometry, in vivo ubiquitylation assay, RNA unwinding activity assay Experimental cell research High 17196963
2007 Recombinant DDX39A (DDX39-L) binds RNA, hydrolyzes NTPs in an RNA-dependent manner, and unwinds double-stranded RNA bidirectionally, confirming it is a bona fide RNA helicase with ATPase activity; punctate nuclear localization suggests a role in RNA splicing/export. Recombinant protein biochemistry: RNA-binding assay, NTPase assay, RNA unwinding assay; nuclear localization by fluorescence microscopy Cancer biology & therapy High 17548965
2010 UAP56 (DDX39B) forms the human TREX complex, whereas URH49 (DDX39A) forms a distinct AREX complex with CIP29; genome-wide mRNA targets regulated by each helicase are different, with each controlling distinct subsets of mitotic regulators. Depletion of URH49/DDX39A causes chromosome arm resolution defects and cytokinesis failure, whereas depletion of CIP29 phenocopies URH49 depletion. RNAi knockdown, genome-wide mRNA profiling, fluorescence microscopy of mitotic phenotypes, Co-IP to define complex composition Molecular biology of the cell High 20573985
2004 URH49 (DDX39A) interacts with the mRNA export factor Aly and can rescue loss of Sub2p (yeast UAP56 homolog), demonstrating functional conservation in mRNA splicing and nuclear export. Yeast complementation assay, Co-immunoprecipitation with Aly Nucleic acids research High 15047853
2006 siRNA-mediated reduction of URH49 (DDX39A) expression leads to nuclear accumulation of poly(A)+ mRNA in a speckled pattern, demonstrating its essential role in nuclear mRNA export; combined depletion of both UAP56 and URH49 causes cell death and major mRNA export block. RNAi knockdown, fluorescence in situ hybridization (FISH) of poly(A)+ RNA Gene High 16949219
2011 DDX39A directly interacts with the TRFH domain of TRF2 via an FXLXP motif and is also found in association with catalytically competent telomerase through interaction with hTERT; overexpression causes progressive telomere elongation while shRNA-mediated depletion causes telomere shortening, DNA-damage foci at telomeres, and aberrant telomere structure. Co-immunoprecipitation, motif mapping, shRNA knockdown, telomere FISH, telomere length assay, telomere dysfunction-induced foci (TIF) assay Aging cell High 21388492
2011 MxA (antiviral GTPase) directly interacts with URH49 (DDX39A) and UAP56 in vitro using purified recombinant proteins; MxA-URH49 complexes localize to the perinuclear region, suggesting MxA exerts antiviral activity by sequestering these RNA helicases. Immunoprecipitation, in vitro binding assay with purified recombinant proteins, co-localization by fluorescence microscopy The Journal of biological chemistry High 21859714
2011 UAP56 and URH49 (DDX39A) exhibit CRM1-independent nucleocytoplasmic shuttling activity; the shuttling region of UAP56 maps to C-terminal amino acids 195–428; the region required for intranuclear localization maps to aa 81–381; interaction with REF requires aa 51–428; human UAP56 shuttles independently of Rae1. Domain mapping with truncation/chimeric mutants, heterokaryon shuttling assay, co-localization studies PloS one Medium 21799930
2020 DDX39A is SUMOylated by SUMO1, 2, and 3; RanBP2 acts as an E3 SUMO1 ligase for DDX39A and enhances SUMO1 modification, which attenuates DDX39A's ability to bind RNA. Viral infection reduces RanBP2-mediated SUMO1 modification of DDX39A, increasing DDX39A binding to TRAF3, TRAF6, and MAVS mRNAs, sequestering them in the nucleus and suppressing innate immune signaling. Co-immunoprecipitation, SUMO modification assays, RNAi knockdown, RNA immunoprecipitation, nuclear/cytoplasmic fractionation Journal of immunology High 32393512
2020 URH49 (DDX39A) forms an Apo-AREX complex containing CIP29 but not ALYREF or the THO subcomplex in ATP-depleted conditions; upon ATP addition, the Apo-AREX complex is remodeled to an ATP-TREX-like complex containing THO subcomplex, ALYREF, and CIP29; URH49-dependent mRNA export is achieved through NXF1. ATP depletion/addition complex assembly assays, Co-IP, knockdown with cytokinesis phenotype readout Biochimica et biophysica acta. Gene regulatory mechanisms High 31917363
2016 Simultaneous knockdown of DDX39A and its paralog DDX39B drastically and selectively downregulates AR-V7 mRNA expression in AR-V7-positive prostate cancer cell lines, identifying DDX39A as a regulator of androgen receptor splice variant generation. shRNA library screen, RNAi knockdown, RT-PCR/qPCR Biochemical and biophysical research communications Medium 28025139
2018 DDX39A overexpression increases β-catenin nuclear accumulation and upregulates Wnt/β-catenin target genes in HCC cells; knockdown of TCF4 and LEF1 in DDX39A-overexpressing cells inhibits Wnt pathway target genes and reduces invasion, placing DDX39A upstream of β-catenin/TCF4/LEF1 in Wnt signaling. Overexpression and knockdown studies, nuclear/cytoplasmic fractionation, Western blot, Wnt target gene RT-PCR, co-knockdown epistasis Cell death & disease Medium 29867138
2021 ECD (mammalian ecdysoneless protein) physically interacts with DDX39A and is required for mRNA nuclear export; ECD knockdown blocks mRNA nuclear export, which is rescued by full-length ECD but not by an ECD mutant defective in DDX39A interaction, establishing DDX39A as a functional partner of ECD in mRNA export. Co-immunoprecipitation, knockdown rescue experiment, FISH for mRNA localization Molecular and cellular biology High 33941617
2023 Upon chikungunya virus (CHIKV) infection, the predominantly nuclear DDX39A accumulates in the cytoplasm; DDX39A binds CHIKV genomic RNA, specifically interacting with the 5' conserved sequence element (5'CSE), and this interaction is essential for antiviral activity; DDX39A inhibits alphavirus replication independently of the canonical interferon pathway. Genetic screen, RNAi/overexpression functional assays, RNA immunoprecipitation, biochemical binding assays, live-cell imaging of relocalization, IFN-independent antiviral assay Molecular cell High 37949067
2024 Upon replication stress, DDX39A resolves RNA-DNA hybrids at stalled replication forks; DDX39A is a RAD51-associated protein that binds stalled forks and unwinds replication fork-associated RNA-DNA hybrids (RF-RDs), facilitating regulated DNA2-mediated DNA resection and fork restart; loss of DDX39A enhances fork protection in BRCA1/2-deficient cells, conferring chemoresistance. Co-immunoprecipitation with RAD51, proximity ligation assays at forks, DNA fiber assay, DRIP-seq (RNA-DNA hybrid mapping), DDX39A knockdown/overexpression with fork restart readouts Molecular cell High 39706185 39706186
2024 DDX39A (URH49) loads PHAX onto U snRNA in an ATP-dependent manner, stimulating RNA binding of PHAX (a U snRNA export adapter), thereby facilitating spliceosomal U snRNA nuclear export; ALYREF acts as a bridge between PHAX and UAP56/DDX39B in this pathway. In vitro reconstitution assay for ATP-dependent U snRNA export factors, RNA-protein loading assay, Co-IP Nucleic acids research High 39011894
2024 DDX39A and DDX39B have significant redundancy in alternative splicing targets genome-wide, but specific pre-mRNAs require one or the other paralog; DDX39A cannot complement DDX39B-dependent splicing of IL7R exon 6; cassette exons specifically dependent on DDX39B have U-poor/C-rich polypyrimidine tracts upstream, establishing this sequence feature as a determinant of DDX39B (not DDX39A) dependency. RNAi knockdown, RNA-seq, splicing minigene assays, paralog-specific complementation Nucleic acids research High 38801080
2024 SNRPD2 (PD2), together with HNRNPL, modulates DDX39A intron retention to produce a short DDX39A variant (39A_S); 39A_S mediates MYC mRNA nuclear export to maintain high MYC protein expression; MYC in turn potentiates PD2 transcription, forming a positive feedback loop in HCC. RNA-seq, RIP-qPCR, Co-IP, mRNA export fractionation assay, knockdown/overexpression, xenograft models Advanced science Medium 39018261
2024 UAP56 (DDX39B) and URH49 (DDX39A) have distinct structural features in their apo forms that determine their respective complex formation (apo-TREX vs apo-AREX); chimeric mutant analysis identified that the terminal regions of UAP56 and the C-terminal region of URH49 are indispensable for their respective complex assembly, and a specific C-terminal amino acid in UAP56 is critical for its complex formation and mRNA export activity. Crystal/structural analysis of UAP56 and URH49, chimeric mutant construction, Co-IP complex assembly assays, mRNA export assays Nature communications High 38225262
2024 In mouse ESCs, Erk2 phosphorylates Ddx39 on Y132 and Y138; phosphorylated Ddx39 promotes recruitment of Hat1 to acetylate H3K27 and activate differentiation genes; Ddx39 is recruited to telomeres by Trf1, disrupts Trf1-mediated DNA loops and suppresses ALT activity; phosphorylation of Ddx39 by Erk2 weakens its interaction with Trf1, releasing it from telomeres and allowing ALT-mediated telomere elongation. Co-IP/MS for Erk2 interactors, in vitro kinase assay, Ddx39 KO ESCs, ChIP, H3K27ac analysis, telomere FISH/CO-FISH for ALT activity Cell death and differentiation High 39107495
2025 A pathogenic DDX39A variant (K137Q) fails to interact with the TREX component THOC1, destabilizing TREX complex integrity; mutant DDX39A-K137Q displays aberrant nuclear clumping rather than normal nuclear distribution; structural modeling shows Lys137 mediates critical inter- and intra-molecular interactions disrupted by K137Q substitution; mutant cells show nuclear lamina disorganization and increased cell death. Patient-derived fibroblast functional studies, Co-IP (DDX39A-K137Q vs THOC1), structural modeling, nuclear morphology analysis, transcriptomic analysis Clinical genetics Medium 40726340
2026 DDX39A directly binds SP1 mRNA, stabilizing it and enhancing SP1 translation efficiency; increased SP1 protein then transcriptionally activates Ku70 (a key NHEJ component) by binding the −223/−214 bp region of the Ku70 promoter, contributing to radioresistance in ESCC; this DDX39A–SP1–Ku70 axis is necessary and sufficient to mediate radioresistance. RIP-qPCR (DDX39A binding to SP1 mRNA), ChIP-qPCR (SP1 on Ku70 promoter), dual-luciferase reporter assay, knockdown/rescue, proteomics, xenograft models Cellular oncology Medium 41984289
2026 DDX39A regulates alternative splicing of WISP1 pre-mRNA, stabilizing it; DDX39A KD reduces the oncogenic WISP1 isoform, suppressing AKT signaling; secreted WISP1 acts as a paracrine signal promoting immunosuppressive tumor-associated macrophage polarization; Fluphenazine hydrochloride binds to and inhibits DDX39A. RNA-seq, RIP-seq, knockdown in vitro and in vivo, WISP1 isoform analysis, AKT pathway readouts, macrophage co-culture assay, drug-target interaction (binding assay) Oncogene Medium 41772197
2025 DDX39A promotes exon skipping of ITGA6 pre-mRNA to produce the oncogenic ITGA6A transcript; SNRPB depletion induces DDX39A intron retention at introns 6 and 8, generating a noncoding transcript subject to nonsense-mediated decay; DDX39A knockdown reduces proliferative and metastatic capacities downstream of SNRPB overexpression. RNA-seq (splicing analysis), RNAi knockdown, antisense oligonucleotide silencing, CDX/PDX models, epistasis between SNRPB and DDX39A Oncogene Medium 40216968

Source papers

Stage 0 corpus · 38 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2010 The closely related RNA helicases, UAP56 and URH49, preferentially form distinct mRNA export machineries and coordinately regulate mitotic progression. Molecular biology of the cell 87 20573985
2006 Intracellular characterization of DDX39, a novel growth-associated RNA helicase. Experimental cell research 70 17196963
2004 Growth-regulated expression and G0-specific turnover of the mRNA that encodes URH49, a mammalian DExH/D box protein that is highly related to the mRNA export protein UAP56. Nucleic acids research 66 15047853
2006 Nuclear localization of poly(A)+ mRNA following siRNA reduction of expression of the mammalian RNA helicases UAP56 and URH49. Gene 63 16949217
2011 Requirement of DDX39 DEAD box RNA helicase for genome integrity and telomere protection. Aging cell 55 21388492
2007 DDX39, upregulated in lung squamous cell cancer, displays RNA helicase activities and promotes cancer cell growth. Cancer biology & therapy 51 17548965
2018 DDX39 promotes hepatocellular carcinoma growth and metastasis through activating Wnt/β-catenin pathway. Cell death & disease 50 29867138
2016 The RNA helicase DDX39B and its paralog DDX39A regulate androgen receptor splice variant AR-V7 generation. Biochemical and biophysical research communications 50 28025139
2011 Interferon-induced antiviral protein MxA interacts with the cellular RNA helicases UAP56 and URH49. The Journal of biological chemistry 35 21859714
2020 SUMOylation of DDX39A Alters Binding and Export of Antiviral Transcripts to Control Innate Immunity. Journal of immunology (Baltimore, Md. : 1950) 27 32393512
2011 The cellular DExD/H-box RNA-helicases UAP56 and URH49 exhibit a CRM1-independent nucleocytoplasmic shuttling activity. PloS one 22 21799930
2023 The RNA helicase DDX39A binds a conserved structure in chikungunya virus RNA to control infection. Molecular cell 20 37949067
2013 Up-regulation of DDX39 in human malignant pleural mesothelioma cell lines compared to normal pleural mesothelial cells. Anticancer research 16 23749908
2024 Intron Retention of DDX39A Driven by SNRPD2 is a Crucial Splicing Axis for Oncogenic MYC/Spliceosome Program in Hepatocellular Carcinoma. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 14 39018261
2024 Parsing the roles of DExD-box proteins DDX39A and DDX39B in alternative RNA splicing. Nucleic acids research 13 38801080
2021 The Mammalian Ecdysoneless Protein Interacts with RNA Helicase DDX39A To Regulate Nuclear mRNA Export. Molecular and cellular biology 13 33941617
2015 Identification of DDX39A as a Potential Biomarker for Unfavorable Neuroblastoma Using a Proteomic Approach. Pediatric blood & cancer 13 26469522
2024 Structural differences between the closely related RNA helicases, UAP56 and URH49, fashion distinct functional apo-complexes. Nature communications 12 38225262
2020 Phosphosite Analysis of the Cytomegaloviral mRNA Export Factor pUL69 Reveals Serines with Critical Importance for Recruitment of Cellular Proteins Pin1 and UAP56/URH49. Journal of virology 12 31969433
2013 Up-regulation of DDX39 in human pancreatic cancer cells with acquired gemcitabine resistance compared to gemcitabine-sensitive parental cells. Anticancer research 12 23898070
2010 Requirement of UAP56, URH49, RBM15, and OTT3 in the expression of Kaposi sarcoma-associated herpesvirus ORF57. Virology 12 20828777
2020 URH49 exports mRNA by remodeling complex formation and mediating the NXF1-dependent pathway. Biochimica et biophysica acta. Gene regulatory mechanisms 11 31917363
2009 RNA helicase Ddx39 is expressed in the developing central nervous system, limb, otic vesicle, branchial arches and facial mesenchyme of Xenopus laevis. Gene expression patterns : GEP 11 19900578
2024 Dynamic control of RNA-DNA hybrid formation orchestrates DNA2 activation at stalled forks by RNAPII and DDX39A. Molecular cell 10 39706186
2010 Binding of the human cytomegalovirus (HCMV) tegument protein UL69 to UAP56/URH49 is not required for efficient replication of HCMV. Journal of virology 9 20610707
2024 DDX39A resolves replication fork-associated RNA-DNA hybrids to balance fork protection and cleavage for genomic stability maintenance. Molecular cell 8 39706185
2024 The RNA helicase DDX39 contributes to the nuclear export of spliceosomal U snRNA by loading of PHAX onto RNA. Nucleic acids research 6 39011894
2024 Novel role for Ddx39 in differentiation and telomere length regulation of embryonic stem cells. Cell death and differentiation 4 39107495
2024 TRAIP enhances progression of tongue squamous cell carcinoma through EMT and Wnt/β-catenin signaling by interacting with DDX39A. BMC cancer 3 39623306
2025 Up-regulated DDX39 in Adrenocortical Carcinoma Is Associated With Patient Survival. Anticancer research 2 39890176
2025 SNRPB-mediated regulation of DDX39A splicing promotes ovarian cancer progression by regulating α6 integrin subunit expression. Oncogene 2 40216968
2025 DDX39 Is Down-regulated in Chromophobe Renal Cell Carcinoma Tissues, Whereas Overexpression Is Associated With Shorter Patient Survival. Anticancer research 1 41151902
2024 Terminal regions of UAP56 and URH49 are required for their distinct complex formation functioning to an essential role in mRNA processing and export. Biochemical and biophysical research communications 1 38377942
2024 Evaluation of protective efficacy of recombinant Toxoplasma gondii DDX39 protein vaccine against acute and chronic T. gondii infection in mice. Acta tropica 1 39461580
2026 The multifunctional RNA helicase DDX39A drives glioblastoma progression by modulating WISP1 alternative splicing that induces an immunosuppressive macrophage polarization. Oncogene 0 41772197
2026 Role of DDX39A in modulating the tumor progression and radiosensitivity in esophageal squamous cell carcinoma. Cellular oncology (Dordrecht, Netherlands) 0 41984289
2025 Pathogenic DDX39A Variant Disrupts Nuclear Homeostasis and Causes an Early-Onset Neurodegenerative Disorder With Cerebral Atrophy. Clinical genetics 0 40726340
2023 The Evil DExH/D: Chikungunya virus runs but cannot hide from DDX39A. Molecular cell 0 37977114