Affinage

CENPE

Centromere-associated protein E · UniProt Q02224

Audit flag: wrong gene
Length
2701 aa
Mass
316.4 kDa
Annotated
2026-06-09
100 papers in source corpus 46 papers cited in narrative 46 extracted findings
Cross-family judge vs UniProt: tie faithfulness: 10/10 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CENP-E (KIF10) is a kinesin-like motor protein that drives chromosome congression and couples kinetochore-microtubule attachment to mitotic checkpoint control during the metaphase-to-anaphase transition (PMID:1406971, PMID:9396744, PMID:10934468). It is expressed cell-cycle-dependently, accumulating through S/G2 to peak in early mitosis and being abruptly degraded at mitotic exit, with antibody perturbation blocking anaphase onset (PMID:2022189, PMID:8207059). As an integral component of the kinetochore fibrous corona, cytoplasmic CENP-E travels along astral microtubules to chromosomes after nuclear envelope breakdown and concentrates at the outermost corona (PMID:9334346). It is a very slow (~2 nm/s), highly processive, plus-end-directed motor with a high-duty-cycle ATPase whose slow microtubule-association step favors binding to stable kinetochore fibers, and whose 230-nm flexible coiled-coil stalk separates motor and kinetochore-binding domains and is required for stable end-on attachment (PMID:18443223, PMID:22637578, PMID:24920822). CENP-E positions chromosomes at the metaphase plate by capturing lateral microtubules and towing polar chromosomes, then converts to a bidirectional tip-tracker that maintains attachment to assembling and disassembling plus ends, with both motor and tail domains contributing (PMID:9363944, PMID:23955301, PMID:23891108). CENP-E directly binds the checkpoint kinase BubR1 in near-stoichiometric complex, and microtubule capture by the CENP-E motor silences BubR1-dependent checkpoint signaling while BubR1 reciprocally phosphorylates CENP-E to switch it from lateral transporter to plus-end tip-tracker (PMID:10934468, PMID:16144904, PMID:31201382). Its activity is multiply regulated: full-length CENP-E is autoinhibited by its tail, relieved by MPS1 or CDK1-cyclin B phosphorylation (PMID:18342609); an Aurora/PP1 switch controls congression versus stable attachment, with distinct Aurora A and Aurora B phosphorylation events governing corona dynamics (PMID:20691903, PMID:37658044); and CDK1/MPF phosphorylation of a C-terminal domain suppresses microtubule cross-linking until anaphase (PMID:8023161). Kinetochore targeting requires SUMO-2/3 chain binding via a SIM motif, dependent on poly-SUMOylation of Nuf2 and SUMOylated NKAP (PMID:18374647, PMID:33910471, PMID:27694884). CENP-E additionally recruits CLASP1/2 to regulate microtubule flux, scaffolds dynein/RZZS recruitment to the corona, and forms a complex with PRC1 for central spindle assembly (PMID:19733075, PMID:37984321, PMID:31174204). Genetic deletion in mouse cells destabilizes kinetochore microtubule capture and causes chromosome missegregation and chromosomal instability (PMID:12361599).

Mechanistic history

Synthesis pass · year-by-year structured walk · 22 steps
  1. 1992 High

    Establishing CENP-E as a kinesin-family motor that cycles through mitosis explained how a centromere protein could physically move chromosomes, framing it as a mechanochemical effector rather than a passive scaffold.

    Evidence Molecular cloning, sequence analysis, and immunofluorescence cell-cycle localization

    PMID:1406971 PMID:2022189

    Open questions at the time
    • Directionality and in vitro motility not yet demonstrated
    • Kinetochore-binding domain unmapped
  2. 1994 High

    Defining timed accumulation and abrupt degradation, plus MPF phosphorylation of a microtubule cross-linking domain, showed CENP-E activity is temporally gated within the mitotic proteolytic and kinase cascade.

    Evidence Elutriation synchronization, pulse-chase immunoblotting, and in vitro MPF kinase/microtubule cross-linking assays

    PMID:8023161 PMID:8207059

    Open questions at the time
    • Ubiquitin ligase responsible for degradation not identified here
    • Physiological cross-linking targets at anaphase undefined
  3. 1997 High

    Immunodepletion, antibody injection, and immuno-EM established CENP-E as a plus-end-directed motor in the kinetochore corona essential for aligning chromosomes at the metaphase plate.

    Evidence Xenopus egg extract depletion, in vitro polarity/motility assays, antibody microinjection, and immunoelectron microscopy

    PMID:9334346 PMID:9363944 PMID:9396744

    Open questions at the time
    • How motor activity is coupled to kinetochore tethering not resolved
    • Lateral vs end-on attachment modes not yet distinguished
  4. 1998 High

    Mapping a mitosis-specific kinetochore-binding domain and identifying BubR1 and CENP-F partners revealed CENP-E as part of a motor-kinase complex assembled in defined order.

    Evidence Yeast two-hybrid screen, co-immunoprecipitation from HeLa cells, and sequential-assembly immunofluorescence

    PMID:9763420

    Open questions at the time
    • Functional consequence of BubR1 binding not yet tested
    • Direct vs indirect nature of CENP-F association unclear
  5. 2000 High

    Loss-of-function in cells and extracts coupled to stoichiometric BubR1 association established CENP-E as the link transducing microtubule-attachment status into checkpoint signaling.

    Evidence Antisense suppression, immunodepletion with MAD2 rescue epistasis, and immunoprecipitation

    PMID:10934468 PMID:11030625

    Open questions at the time
    • Whether CENP-E activates BubR1 kinase not yet shown
    • Molecular switch coupling capture to silencing undefined
  6. 2003 High

    Sequential kinase-dependence experiments placed Aurora B, Bub1, and MPS1 upstream of CENP-E kinetochore recruitment, defining the assembly hierarchy for the motor.

    Evidence Pharmacological inhibition (ZM447439), RNAi, and immunofluorescence localization

    PMID:12686615 PMID:12719470 PMID:15020684

    Open questions at the time
    • Direct molecular receptors for CENP-E at the kinetochore not identified
    • Whether kinase effects are direct or via intermediate proteins unclear
  7. 2004 High

    The motor-domain crystal structure with MgADP and a docked linker confirmed at atomic resolution the plus-end directionality inferred from motility assays.

    Evidence X-ray crystallography at 2.5 Angstrom of the human motor domain and linker

    PMID:15236970

    Open questions at the time
    • Full-length architecture and tail regulation not captured
    • Microtubule-bound state not resolved
  8. 2005 High

    Reconstitution of a BubR1-CENP-E-microtubule ternary complex showed CENP-E binding activates BubR1 kinase and that motor-mediated microtubule capture silences this signaling, defining the mechanistic core of attachment-to-checkpoint coupling.

    Evidence In vitro kinase assays with purified components plus motorless-fragment expression in cells

    PMID:16144904

    Open questions at the time
    • Reciprocal phosphorylation of CENP-E by BubR1 not yet demonstrated
    • In vivo relevance of silencing step partly inferred
  9. 2002 High

    Mouse gene deletion provided in vivo proof that CENP-E is required for stable kinetochore microtubule capture and chromosome stability, linking its molecular activity to chromosomal instability.

    Evidence Conditional knockout with EM quantification of kinetochore microtubules and chromosome counting

    PMID:12361599

    Open questions at the time
    • Molecular basis of microtubule-number reduction not dissected
    • Tissue-specific requirements not fully mapped
  10. 2008 High

    Single-molecule and structural work defined CENP-E as a slow, highly processive motor with a long flexible stalk that is autoinhibited by its tail and relieved by MPS1/CDK1 phosphorylation, explaining how its activity is spatially and temporally licensed.

    Evidence Single-molecule motility, EM of full-length Xenopus protein, tail-deletion constructs, and kinase assays

    PMID:11382767 PMID:18342609 PMID:18443223

    Open questions at the time
    • In vivo phosphorylation sites mediating relief not fully mapped
    • Coordination of autoinhibition with kinetochore loading unclear
  11. 2008 High

    Identifying SUMO-2/3 chain binding via a SIM motif as essential for kinetochore targeting revealed a non-phosphorylation PTM-reader mechanism for CENP-E localization.

    Evidence SUMOylation and SUMO-chain binding assays, SIM mutant analysis, and global SUMOylation inhibition

    PMID:18374647

    Open questions at the time
    • SUMOylated kinetochore receptor not yet identified
    • Whether CENP-E itself or its receptor carries the chain unresolved
  12. 2009 High

    Enzymological kinetics and CLASP recruitment work explained how CENP-E processivity favors stable fibers and linked the motor to kinetochore microtubule flux regulation.

    Evidence Pre-steady-state ATPase kinetics, MS-based interactor identification, co-IP, and FRAP flux measurements

    PMID:19733075 PMID:22637578

    Open questions at the time
    • How the slow association step is structurally encoded unclear
    • Mechanism by which CENP-E delivers CLASPs not defined
  13. 2010 High

    An Aurora/PP1 phosphorylation switch and demonstration of ATP-dependent plus-end elongation showed how CENP-E affinity for microtubules is tuned to first tow polar chromosomes then form stable attachments.

    Evidence In vitro kinase and single-molecule assays, PP1-binding mutants, phospho-antibodies, and microtubule polymerization assays

    PMID:20691903 PMID:20797864

    Open questions at the time
    • The plus-end elongation activity (Medium) is from a single lab and not extensively corroborated
    • Coupling of PP1 rebinding to end-on conversion in vivo partly inferred
  14. 2013 High

    Single-molecule trapping, attachment-intermediate imaging, and stalk-truncation studies defined how CENP-E converts from a lateral transporter and wall-tether into a bidirectional tip-tracker dependent on motor, tail, and stalk domains.

    Evidence Laser trapping, computational modeling, live-cell attachment-intermediate imaging, and Bonsai stalk mutant assays

    PMID:23891108 PMID:23892111 PMID:23955301 PMID:24920822

    Open questions at the time
    • Molecular trigger of the lateral-to-end-on conversion not fully resolved
    • Quantitative contribution of the non-kinesin microtubule-binding domain in vivo unclear
  15. 2018 High

    Biochemical reconstitution established that CENP-E binds BubR1 directly through a dimeric coiled-coil engaging the BubR1 kinase domain, and that CENP-E loads onto kinetochores independently of the RZZ complex.

    Evidence Reconstitution with purified proteins, co-IP, RNAi epistasis, and domain mapping

    PMID:29748388

    Open questions at the time
    • Hierarchy relative to SUMO and ubiquitin receptors not integrated
    • Whether BubR1 binding contributes to CENP-E retention unclear
  16. 2019 High

    Demonstrating BubR1 is a genuine kinase that phosphorylates CENP-E to switch it from transporter to tip-tracker closed the reciprocal regulatory loop with the checkpoint kinase and tied it to central spindle assembly.

    Evidence BubR1 kinase domain crystal structure, in vitro kinase and single-molecule assays, live imaging, and inhibitor design

    PMID:31201382

    Open questions at the time
    • Phospho-site identity on CENP-E not fully defined here
    • In vivo timing relative to attachment status partly modeled
  17. 2015 Medium

    Identification of TRAMM/TrappC12 and CTCF as CENP-E recruiters expanded the set of localization determinants beyond canonical kinetochore proteins to include phospho-regulated and chromatin-anchored pathways.

    Evidence Co-IP, ChIP, phosphomimetic/non-phosphorylatable and dominant-negative constructs, and immunofluorescence

    PMID:25918224 PMID:26321640

    Open questions at the time
    • TRAMM and CTCF links rest on single-lab co-IP plus mutant assays without reconstitution
    • How these pathways integrate with SUMO/ubiquitin recruitment unclear
  18. 2016 Medium

    Showing SUMOylated NKAP, recruited by Bub3, is required to stabilize CENP-E-BubR1 binding connected the SUMO-dependent localization mechanism to a specific kinetochore adaptor.

    Evidence RNAi, SUMOylation assays, co-IP, and SUMOylation-deficient mutant rescue

    PMID:27694884

    Open questions at the time
    • Single-lab evidence; relationship to the CENP-E SIM not fully reconciled
    • Whether NKAP SUMOylation is the chain read by the CENP-E SIM untested
  19. 2019 Medium

    LUBAC-catalyzed linear ubiquitination read by KNL1 was shown to anchor CENP-E specifically at attached kinetochores, adding a ubiquitin-dependent retention mechanism coupled to the KMN network.

    Evidence Linear ubiquitination assays, KNL1 interaction assays, RNAi/knockout, and attachment-status immunofluorescence

    PMID:30655516

    Open questions at the time
    • Single-lab demonstration; the ubiquitinated residues on CENP-E not mapped
    • How this coexists with SUMO-dependent targeting unclear
  20. 2020 Medium

    Identifying a CENP-E-PRC1 complex established a role for CENP-E in organizing kinetochore microtubules into stable midzone arrays at the metaphase-anaphase transition.

    Evidence Biotinylated-syntelin affinity purification, co-IP, chemical inhibition, and light-sheet live imaging

    PMID:31174204

    Open questions at the time
    • Single-lab affinity-capture interaction; directness of CENP-E-PRC1 binding unclear
    • Whether motor activity is needed for midzone assembly untested
  21. 2023 High

    Distinct Aurora A (pole) and Aurora B (kinetochore) phosphorylation events, and a CENP-E/RZZS platform for dynein recruitment, revealed how CENP-E spatially controls fibrous corona dynamics and dynein loading.

    Evidence Phospho-specific antibodies, Aurora inhibitors, phospho-mutant constructs, MPS1 inhibition, RZZS phosphomimetics, and live imaging

    PMID:37658044 PMID:37984321

    Open questions at the time
    • The EMBO dynein-platform study (Medium) is single-lab
    • How corona disassembly timing integrates with checkpoint silencing unresolved
  22. 2023 Medium

    Demonstrating HPV16 E6/E6AP-driven proteasomal degradation of CENP-E producing chromosomal instability connected CENP-E turnover to viral oncogenesis independently of p53.

    Evidence HPV16 E6 expression, proteasome inhibitor rescue, E6AP knockdown in p53-independent cells, and congression imaging

    PMID:36989302

    Open questions at the time
    • Single-lab; direct CENP-E ubiquitination by E6AP not shown
    • Relevance to spontaneous tumor CIN beyond HPV context unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the multiple parallel recruitment inputs (SUMO-2/3 chains, linear ubiquitin/KNL1, BubR1, NKAP, TRAMM, CTCF, RZZS) are integrated and ordered to set CENP-E levels, residence time, and attachment-mode at a single kinetochore remains unresolved.
  • No unified model reconciling SUMO, ubiquitin, and protein-protein receptor pathways
  • Quantitative stoichiometry of CENP-E loading mechanisms unknown
  • Direct in vivo phospho-site map across regulatory kinases incomplete

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003774 cytoskeletal motor activity 4 GO:0060090 molecular adaptor activity 4 GO:0008092 cytoskeletal protein binding 3 GO:0140657 ATP-dependent activity 2
Localization
GO:0005694 chromosome 2 GO:0005815 microtubule organizing center 2 GO:0005856 cytoskeleton 2
Pathway
R-HSA-1640170 Cell Cycle 4 R-HSA-1852241 Organelle biogenesis and maintenance 2
Partners
BUBR1CENP-FCLASP1KIF18AKNL1PRC1SKAPSKP1
Complex memberships
BubR1-CENP-E complexkinetochore fibrous corona

Evidence

Reading pass · 46 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1991 CENP-E is a novel ~250-300 kDa chromosome scaffold-associated protein that localizes to centromeres during prometaphase and metaphase, relocalizes to the spindle midplate/midbody at anaphase/telophase, and is absent during interphase. Microinjection of anti-CENP-E antibody (mAb177) into metaphase cells blocks or significantly delays progression into anaphase, establishing a required role in the metaphase-to-anaphase transition. Monoclonal antibody generation, immunofluorescence cell cycle staging, microinjection of antibody into live cells The EMBO journal High 2022189
1992 CENP-E is a kinesin-like motor protein (Mr ~312,000) that accumulates during G2 phase, associates with kinetochores during congression, relocalizes to the spindle midzone at anaphase, and is quantitatively degraded at the end of cell division, suggesting roles in chromosome movement and/or spindle elongation. Molecular cloning, sequence analysis, immunofluorescence cell-cycle localization, immunoblotting Nature High 1406971
1994 CENP-E levels increase progressively through S and G2 (peaking ~22,000 molecules/cell in early mitosis) due to stabilization, then are abruptly degraded (>10-fold) at the end of mitosis (after cyclin B). This degradation is independent of cytokinesis and defines a fourth point in a mitotic cascade of timed proteolysis. Centrifugal elutriation cell synchronization, immunoblotting, [35S]methionine pulse labeling, cytochalasin block The Journal of cell biology High 8207059
1994 CENP-E contains a 99-amino acid C-terminal domain that cross-links microtubules at anaphase. Phosphorylation of this C-terminal domain by MPF (CDK1/cyclin B) inhibits its microtubule-binding activity, suppressing CENP-E microtubule cross-linking until anaphase when MPF activity is lost. In vitro microtubule binding/cross-linking assay, MPF phosphorylation assay, domain mapping Science High 8023161
1995 CENP-E helps couple chromosomes to depolymerizing microtubules in vitro. Antibodies to the CENP-E neck region completely block depolymerization-dependent chromosome motion, while antibodies to the head or tail slow it ~3-fold, demonstrating domain-specific contributions to microtubule end-tracking. In vitro chromosome motility assay with depolymerizing microtubules, domain-specific antibody inhibition, immunoblotting The Journal of cell biology High 7822408
1997 CENP-E is a plus-end-directed microtubule motor essential for positioning chromosomes at the metaphase plate. Immunodepletion from Xenopus egg extracts prevents chromosome alignment, and antibody addition to the extracts recapitulates this phenotype. In vitro motility assays confirm plus-end directionality. Immunodepletion from Xenopus egg extracts, antibody addition, in vitro microtubule gliding/polarity assays Cell High 9363944
1997 CENP-E function at kinetochores is essential for chromosome alignment: antibody microinjection prevents kinetochore assembly of CENP-E, causing two defects—monopolar chromosomes cannot establish bipolar connections, and bipolar chromosomes with normal oscillation velocities fail to align. Overexpression of an N-terminal motor-deleted truncation competitively blocks endogenous CENP-E from kinetochores and phenocopies antibody injection, implicating motor domain activity. Affinity-purified antibody microinjection, dominant-negative truncation overexpression, immunofluorescence, video microscopy The Journal of cell biology High 9396744
1997 CENP-E is an integral component of kinetochore corona fibers. Immunoelectron microscopy shows cytoplasmic CENP-E travels along astral microtubules toward chromosomes after nuclear envelope breakdown, then localizes to the outermost kinetochore corona extending ≥50 nm from the outer plate, intertwining with spindle microtubules throughout congression and anaphase A. Immunoelectron microscopy (immuno-EM), immunofluorescence The Journal of cell biology High 9334346
1998 A 350-amino acid domain in CENP-E mediates kinetochore binding specifically in mitosis. Yeast two-hybrid screening with this domain identified interactions with CENP-F and hBUBR1; co-immunoprecipitation from HeLa cells confirmed CENP-E and hBUBR1 associate, suggesting a motor-kinase complex. CENP-F, hBUBR1, and CENP-E assemble onto kinetochores in sequential order. Yeast two-hybrid screen, co-immunoprecipitation from HeLa cells, domain mapping, immunofluorescence The Journal of cell biology High 9763420
1998 Active MAP kinase (ERK1/2) localizes to kinetochores during mitosis and associates preferentially with CENP-E. CENP-E is phosphorylated in vitro by MAP kinase on sites known to regulate its microtubule interactions. Immunofluorescence with phospho-MAP kinase antibody, co-immunoprecipitation, in vitro kinase assay The Journal of cell biology Medium 9744883
1999 hBUBR1 checkpoint kinase activity is detected only after mitotic entry and is stimulated by spindle disruption. hBUBR1 monitors kinetochore activities that depend on CENP-E, and associates with the APC/cyclosome in mitotically arrested cells, suggesting dual checkpoint functions. Kinase assays, co-immunoprecipitation, immunofluorescence, cell arrest assays The Journal of cell biology Medium 10477750
2000 CENP-E suppression by antisense yields chronically mono-oriented chromosomes, spindle pole fragmentation, and profound checkpoint activation. Immunoprecipitation reveals nearly stoichiometric association of CENP-E with the checkpoint kinase BubR1 during mitosis, establishing CENP-E as a link between spindle microtubule attachment and mitotic checkpoint signaling. Antisense suppression, immunoprecipitation, immunofluorescence, spindle pole analysis Nature cell biology High 10934468
2000 Farnesyl transferase inhibitors (FTIs) block farnesylation of CENP-E and CENP-F in DLD-1 cells, but FTI treatment does not prevent kinetochore localization of CENP-E; instead, it alters the association between CENP-E and microtubules. In vitro farnesyl transferase assays with CENP-E CAAX peptides, immunohistochemistry, FTI treatment of cell lines The Journal of biological chemistry Medium 10852915
2000 CENP-E is required for establishing and maintaining the mitotic checkpoint in Xenopus egg extracts. Immunodepletion or antibody inhibition of CENP-E prevents extract arrest in response to spindle damage; adding high levels of soluble MAD2 restores arrest, indicating CENP-E is needed for kinetochore-dependent checkpoint signaling but not downstream steps. CENP-E directly binds BUBR1. Immunodepletion from Xenopus egg extracts, antibody inhibition, MAD2 rescue experiment Cell High 11030625
2002 Selective gene deletion of mouse CENP-E in primary cells causes unstable microtubule capture at kinetochores (half the normal microtubule number at aligned chromosomes, none at unaligned polar chromosomes), chromosome missegregation, and chromosomal instability. Embryonic CENP-E deletion leads to early developmental arrest. Conditional/selective gene knockout in mouse, electron microscopy of kinetochore microtubules, immunofluorescence, chromosome counting Developmental cell High 12361599
2003 Aurora B kinase activity is required for kinetochore localization of CENP-E (as well as BubR1 and Mad2). Inhibition of Aurora B with ZM447439 diminishes CENP-E kinetochore localization; RNAi confirms Aurora B (not Aurora A) mediates this effect. Loss of Aurora B compromises spindle checkpoint and chromosome alignment. Small-molecule Aurora kinase inhibitor (ZM447439), RNAi, immunofluorescence The Journal of cell biology High 12719470
2003 Human MPS1 (TTK) kinase is required for mitotic arrest induced by loss of CENP-E from kinetochores. MPS1 kinetochore localization in CENP-E-defective cells is sensitive to microtubule occupancy. MPS1 is required for kinetochore localization of MAD1 and MAD2 but not hBUB1 or hBUBR1. siRNA depletion, immunofluorescence, dominant-negative kinetochore targeting domain expression Molecular biology of the cell Medium 12686615
2004 Crystal structure of the human CENP-E motor domain and linker region at 2.5 Å resolution with MgADP bound in the active site. The linker region adopts a 'docked' conformation identical to that of plus-end-directed conventional kinesin, consistent with CENP-E being a plus-end motor. X-ray crystallography (2.5 Å resolution) Journal of molecular biology High 15236970
2004 Bub1 kinase is required at the kinetochore for subsequent localization of CENP-E (as well as Cenp-F, BubR1, and Mad2) in human somatic cells. RNAi depletion of Bub1 prevents CENP-E kinetochore recruitment and increases lagging chromosomes, suggesting Bub1 acts upstream of CENP-E in the kinetochore assembly pathway. RNAi in human somatic cells, immunofluorescence quantification of kinetochore protein levels Journal of cell science High 15020684
2005 Direct binding of BubR1 to CENP-E activates BubR1 kinase activity. A motorless CENP-E fragment constitutively activates BubR1 at kinetochores, producing checkpoint signaling not silenced by microtubule capture or tension. In a purified ternary system, microtubule capture by the CENP-E motor domain silences BubR1 kinase activity in the BubR1-CENP-E-microtubule complex, revealing CENP-E as the signal transducer responsible for silencing checkpoint signaling upon kinetochore microtubule capture. In vitro kinase assay with purified BubR1, CENP-E, and microtubules; motorless CENP-E fragment expression; immunofluorescence The Journal of cell biology High 16144904
2008 CENP-E is specifically modified by SUMO-2/3 (not SUMO-1) during mitosis. CENP-E possesses SUMO-2/3 polymeric chain-binding activity via a SUMO-interacting motif (SIM) that is essential for its kinetochore localization. Global inhibition of SUMOylation causes prometaphase arrest due to failure of CENP-E targeting to kinetochores. SUMOylation assays, SUMO-2/3 binding assays, SIM mutant analysis, global SUMOylation inhibition, immunofluorescence Molecular cell High 18374647
2008 Full-length Xenopus CENP-E is an autoinhibited motor: its tail domain directly interacts with and completely blocks the motor domain's motility in vitro. Phosphorylation of the CENP-E tail by MPS1 or CDK1-cyclin B relieves this autoinhibition, activating CENP-E motility. In vitro motility assays with purified full-length Xenopus CENP-E, tail-deletion constructs, MPS1 and CDK1-cyclin B kinase assays Molecular cell High 18342609
2008 CENP-E is a very slow (~2 nm/s), highly processive plus-end-directed motor with a 230-nm flexible coiled-coil stalk separating its kinetochore-binding and motor domains. Single-molecule assays show long microtubule attachment times. This processivity and flexibility support roles in chromosome congression and kinetochore tethering to dynamic microtubule plus ends. Single-molecule motility assays, electron microscopy of full-length Xenopus CENP-E, fluorescence imaging The Journal of cell biology High 18443223
2008 Native purified CENP-E from mitotic HeLa cells does not induce microtubule gliding but retains microtubule-binding activity, suggesting that the native form is in a regulated (possibly autoinhibited) state. Native protein purification from mitotic HeLa cells, microtubule gliding assay, microtubule binding assay The Journal of biological chemistry Medium 11382767
2009 CENP-E motor domain associates with microtubules unusually slowly (0.08 µM⁻¹s⁻¹), followed by slow ADP release (0.9 s⁻¹), but fast ATP binding and hydrolysis; motor dissociation rate (~1.4 s⁻¹) matches the stepping rate. This high duty cycle explains CENP-E processivity. The slow microtubule association step is proposed to favor binding to stable kinetochore fibers over dynamic microtubules. Pre-steady-state and steady-state kinetics (stopped-flow, fluorescence), ATPase assays The Journal of biological chemistry High 22637578
2009 CENP-E recruits both CLASP1 and CLASP2 to kinetochores independently of its motor activity or the presence of microtubules, linking CENP-E to regulation of kinetochore microtubule poleward flux and turnover. CENP-E was identified as a CLASP1 interactor by proteomic approach. Proteomic co-purification (MS), co-immunoprecipitation, RNAi, immunofluorescence, microtubule flux/turnover measurements (FRAP) Current biology High 19733075
2010 Aurora kinases A and B phosphorylate a conserved residue on CENP-E. PP1 binds CENP-E via a motif overlapping this phosphorylation site; Aurora phosphorylation disrupts PP1 binding. At spindle poles, Aurora phosphorylation of CENP-E reduces its affinity for individual microtubules, promoting towing of polar chromosomes. Dephosphorylation of CENP-E (or PP1 rebinding) is required for subsequent stable end-on kinetochore-microtubule capture, revealing an Aurora/PP1 switch controlling both congression and stable attachment. In vitro kinase assays, single-molecule motility assays, phospho-specific antibodies, PP1-binding domain mutants, immunofluorescence, live-cell imaging Cell High 20691903
2010 CENP-E promotes microtubule plus-end elongation in vitro in an ATP-dependent manner. ~60% of polarity-marked microtubules show CENP-E-dependent plus-end elongation at ~1.48 µm/30 min. CENP-E localizes to elongating plus ends. The kinetics fit a single exponential (k_obs = 5.1 s⁻¹), suggesting tubulin addition coupled to ATP turnover. In vitro microtubule polymerization assay with purified human CENP-E, real-time fluorescence microscopy, immunolocalization on microtubules Current biology Medium 20797864
2011 CENP-E interacts with SKAP via its C-terminal tail in vitro and in vivo. SKAP depletion by RNAi dramatically reduces inter-kinetochore tension, phenocopying CENP-E depletion. SKAP localizes to kinetochore corona fibers. SKAP and CENP-E synergistically promote microtubule binding in vitro. Co-immunoprecipitation, in vitro pulldown, siRNA, immunoelectron microscopy, microtubule co-sedimentation assay, inter-kinetochore distance measurement The Journal of biological chemistry Medium 22110139
2013 After chromosomes have congressed, CENP-E converts from a lateral microtubule transporter into a bidirectional microtubule tip-tracker that maintains association with both assembling and disassembling microtubule tips. Laser-trapping single-molecule experiments and computational modeling demonstrate this tip-tracking relies on both motor and tail domains. Single-molecule motility assays, laser trapping, computational modeling, fluorescence microscopy Nature cell biology High 23955301
2013 CENP-E tethers laterally attached kinetochores to microtubule walls (wall-tethering), which is a required step prior to end-on conversion. MCAK is separately needed to release lateral microtubules after partial end-on attachment. Together, CENP-E and MCAK define sequential deterministic steps in lateral-to-end-on attachment conversion. High-resolution live-cell imaging assay for attachment intermediates, siRNA depletion of CENP-E and MCAK, fluorescence microscopy Current biology Medium 23891108
2013 The C-terminal non-kinesin microtubule-binding domain of CENP-E binds microtubules with affinity similar to the Ndc80 complex. Electron microscopy shows this domain engages microtubules in a disordered manner with no favored binding geometry, suggesting a role in initial lateral attachments. Microtubule co-sedimentation assay, electron microscopy of microtubule-bound domain Journal of molecular biology Medium 23892111
2014 The elongated coiled-coil stalk of CENP-E is required for stable kinetochore-microtubule end-on attachment. 'Bonsai' CENP-E with significantly shortened stalk but intact motor and tail domains fails to bind microtubules in vitro unless cargo is bound to its tail. In cells, Bonsai CENP-E causes chromosome misalignment and lagging chromosomes, demonstrating the stalk is required for kinetochore-microtubule attachment stability. Stalk-truncation mutant ('Bonsai CENP-E'), in vitro microtubule binding assay, live-cell imaging, immunofluorescence Molecular biology of the cell High 24920822
2016 SUMOylated NKAP protein is required for CENP-E kinetochore localization. NKAP is SUMOylated predominantly during mitosis, and SUMOylation is necessary for NKAP to bind CENP-E. Bub3 recruits NKAP to kinetochores to stabilize CENP-E binding to BubR1. A SUMOylation-deficient NKAP mutant cannot support CENP-E kinetochore localization or chromosome alignment. RNAi, SUMOylation assays, co-immunoprecipitation, NKAP SUMOylation-deficient mutant rescue experiments, immunofluorescence Nature communications Medium 27694884
2018 CENP-E and CENP-F directly and specifically interact with BUBR1 and BUB1, respectively, through biochemical reconstitution. The CENP-E/BUBR1 interaction requires a dimeric coiled-coil in CENP-E and the kinase domain of BUBR1. BUBR1 is dispensable for kinetochore localization of CENP-E, whereas BUB1 is stringently required for CENP-F localization. CENP-E and CENP-F are recruited to kinetochores independently of the RZZ complex. Biochemical reconstitution with purified proteins, co-immunoprecipitation, RNAi, immunofluorescence, domain mapping The Journal of biological chemistry High 29748388
2019 BubR1 is a bona fide kinase that phosphorylates CENP-E, switching it from a laterally-attached microtubule transporter to a plus-end microtubule tip-tracker. BubR1-mediated CENP-E phosphorylation is required for proper microtubule capture at kinetochores and for assembly of the central spindle/midzone at mitotic exit. Computational modeling identified bubristatin as a selective BubR1 kinase antagonist. Crystal structure of Drosophila BubR1 kinase domain, in vitro kinase assay, single-molecule motility assays, live-cell imaging, computational modeling and inhibitor design Cell research High 31201382
2019 LUBAC ubiquitin ligase catalyzes linear ubiquitination of CENP-E, which is specifically required for CENP-E localization at attached kinetochores (but not unattached ones). KNL1 acts as a receptor for linear ubiquitin chains to anchor CENP-E at attached kinetochores. This mechanism promotes chromosome congression by coupling CENP-E to the KMN network. Co-immunoprecipitation, linear ubiquitination assay, KNL1 interaction assay, RNAi/knockout, immunofluorescence with attachment status tracking Nature communications Medium 30655516
2015 TRAMM/TrappC12 interacts with CENP-E and is required for its kinetochore recruitment. TRAMM is phosphorylated early in mitosis and dephosphorylated at anaphase onset; this phosphorylation state correlates with CENP-E association. A phosphomimetic TRAMM mutant recruits CENP-E to kinetochores more efficiently than the non-phosphorylatable form. Co-immunoprecipitation, RNAi, phosphomimetic and non-phosphorylatable mutants, immunofluorescence The Journal of cell biology Medium 25918224
2015 CTCF interacts with CENP-E both in vitro and in vivo via its C-terminal zinc fingers, and recruits CENP-E to pericentric/centromeric DNA early in mitosis. Overexpression of a CENP-E fragment targeted to CTCF sites delays chromosome alignment during mitosis, suggesting physiological relevance of CTCF-mediated CENP-E recruitment. Co-immunoprecipitation, ChIP, in vitro binding assay, dominant-negative fragment overexpression, immunofluorescence Cell reports Medium 26321640
2021 Poly-SUMO-2/3 chain modification of Nuf2 (a kinetochore protein) facilitates CENP-E kinetochore localization and chromosome congression. Nuf2-Ubc9 fusion (stimulating Nuf2 SUMOylation) or Nuf2-SUMO-2 trimer fusion rescues CENP-E kinetochore localization when global SUMOylation is inhibited. The rescue requires a functional SIM in CENP-E. SUMO-2/3 monomer or dimer, or SUMO-1 trimer modifications, cannot rescue, demonstrating specificity for poly-SUMO-2/3 chains. Fusion protein rescue experiments, SUMO chain-specific binding assay, immunofluorescence, global sumoylation inhibition Cell cycle High 33910471
2006 CENP-E interacts with Skp1 at the midbody via its coiled-coil domain (residues 955-1571), and this interaction is required for cytokinesis. Skp1 siRNA causes accumulation of telophase cells with elongated midbodies and elevated CENP-E levels. Overexpression of Skp1 lacking the CENP-E-binding domain confirms that Skp1-CENP-E interaction is essential for faithful cytokinesis, suggesting SCF-mediated degradation of CENP-E at the midbody is required for mitotic exit. Yeast two-hybrid, in vitro binding assay, co-immunoprecipitation, siRNA, immunofluorescence, domain mapping Biochemical and biophysical research communications Medium 16682006
2023 Aurora A phosphorylates CENP-E at spindle poles to promote chromosome congression and prevent accumulation of corona proteins (fibrous corona components) at centrosomes, enabling their redistribution. Aurora B phosphorylates CENP-E at kinetochores to release it from an autoinhibited state and prevent premature removal by dynein, thereby controlling fibrous corona disassembly timing. Phospho-specific antibodies, Aurora kinase inhibitors, dominant-negative and phospho-mutant CENP-E constructs, live-cell imaging, immunofluorescence Nature communications High 37658044
2023 CENP-E is required to retain RZZS (ROD-Zwilch-ZW10-Spindly) at kinetochores when corona assembly is prevented by MPS1 inhibition. With active MPS1, CENP-E is dispensable for corona expansion but strictly required for physiological kinetochore accumulation of dynein-dynactin. CENP-E and the RZZ-Spindly complex form an integrated platform to recruit dynein to the kinetochore corona. MPS1 inhibition, CENP-E depletion/rescue, RZZS phosphomimetic mutants, immunofluorescence quantification The EMBO journal Medium 37984321
2023 HPV16 E6 oncoprotein causes proteasome-dependent degradation of CENP-E through the E6-associated ubiquitin ligase E6AP/UBE3A, producing polar chromosomes and chromosomal instability independently of p53 degradation. HPV16 E6 expression in cell lines, proteasome inhibitor experiments, E6AP knockdown, p53-independent cell lines, chromosome congression imaging Proceedings of the National Academy of Sciences of the United States of America Medium 36989302
2020 CENP-E forms a complex with PRC1 in mitotic cells (identified via biotinylated syntelin affinity matrix). Chemical inhibition of CENP-E in metaphase perturbs temporal assembly of PRC1 to the spindle midzone, preventing accurate central spindle assembly. This identifies a role for CENP-E in organizing kinetochore microtubules into stable midzone arrays at the metaphase-anaphase transition. Affinity purification (biotinylated syntelin), co-immunoprecipitation, chemical inhibition (syntelin), live-cell light sheet microscopy of 3D organoids and 2D culture Journal of molecular cell biology Medium 31174204
2009 KIF18A physically interacts with CENP-E and BubR1 during mitosis (co-immunoprecipitation). KIF18A depletion causes specific downregulation of CENP-E through enhanced protein degradation (not reduced transcription). Ectopic expression of wild-type CENP-E tail domain, but not a corresponding mutant, rescues chromosome congression defects from KIF18A silencing. Co-immunoprecipitation, siRNA, immunofluorescence, protein stability analysis (transcription vs. degradation), rescue by CENP-E tail overexpression Cell cycle Medium 19625775

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2003 Aurora B couples chromosome alignment with anaphase by targeting BubR1, Mad2, and Cenp-E to kinetochores. The Journal of cell biology 1052 12719470
1992 CENP-E is a putative kinetochore motor that accumulates just before mitosis. Nature 375 1406971
1997 CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment. Cell 358 9363944
1991 CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase. The EMBO journal 346 2022189
2000 CENP-E forms a link between attachment of spindle microtubules to kinetochores and the mitotic checkpoint. Nature cell biology 320 10934468
1999 Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC. The Journal of cell biology 314 10477750
2000 Farnesyl transferase inhibitors block the farnesylation of CENP-E and CENP-F and alter the association of CENP-E with the microtubules. The Journal of biological chemistry 292 10852915
2004 Bub1 is required for kinetochore localization of BubR1, Cenp-E, Cenp-F and Mad2, and chromosome congression. Journal of cell science 288 15020684
1997 CENP-E function at kinetochores is essential for chromosome alignment. The Journal of cell biology 276 9396744
2002 Unstable kinetochore-microtubule capture and chromosomal instability following deletion of CENP-E. Developmental cell 267 12361599
1998 Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1. The Journal of cell biology 239 9763420
2001 CENP-E is essential for reliable bioriented spindle attachment, but chromosome alignment can be achieved via redundant mechanisms in mammalian cells. Molecular biology of the cell 220 11553716
2008 SUMO-2/3 modification and binding regulate the association of CENP-E with kinetochores and progression through mitosis. Molecular cell 203 18374647
2010 Aurora kinases and protein phosphatase 1 mediate chromosome congression through regulation of CENP-E. Cell 199 20691903
1997 The microtubule-dependent motor centromere-associated protein E (CENP-E) is an integral component of kinetochore corona fibers that link centromeres to spindle microtubules. The Journal of cell biology 199 9334346
1998 Active MAP kinase in mitosis: localization at kinetochores and association with the motor protein CENP-E. The Journal of cell biology 197 9744883
2001 Spindle checkpoint protein Bub1 is required for kinetochore localization of Mad1, Mad2, Bub3, and CENP-E, independently of its kinase activity. The Journal of cell biology 196 11402067
1995 Identification of centromeric antigens in dicentric Robertsonian translocations: CENP-C and CENP-E are necessary components of functional centromeres. Human molecular genetics 196 8634687
1995 Antibodies to the kinesin motor domain and CENP-E inhibit microtubule depolymerization-dependent motion of chromosomes in vitro. The Journal of cell biology 190 7822408
2000 CENP-E as an essential component of the mitotic checkpoint in vitro. Cell 179 11030625
1997 Localization of CENP-E in the fibrous corona and outer plate of mammalian kinetochores from prometaphase through anaphase. Chromosoma 147 9391217
2003 Human MPS1 kinase is required for mitotic arrest induced by the loss of CENP-E from kinetochores. Molecular biology of the cell 140 12686615
1994 Cyclin-like accumulation and loss of the putative kinetochore motor CENP-E results from coupling continuous synthesis with specific degradation at the end of mitosis. The Journal of cell biology 127 8207059
1994 Mitotic regulation of microtubule cross-linking activity of CENP-E kinetochore protein. Science (New York, N.Y.) 123 8023161
2005 Microtubule capture by CENP-E silences BubR1-dependent mitotic checkpoint signaling. The Journal of cell biology 122 16144904
2008 CENP-E combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether. The Journal of cell biology 118 18443223
2013 Kinetochore kinesin CENP-E is a processive bi-directional tracker of dynamic microtubule tips. Nature cell biology 115 23955301
2013 Lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENP-E and MCAK. Current biology : CB 110 23891108
2004 Gene silencing of CENP-E by small interfering RNA in HeLa cells leads to missegregation of chromosomes after a mitotic delay. Molecular biology of the cell 103 15181147
2009 Motor-independent targeting of CLASPs to kinetochores by CENP-E promotes microtubule turnover and poleward flux. Current biology : CB 102 19733075
2008 Phosphorylation relieves autoinhibition of the kinetochore motor Cenp-E. Molecular cell 89 18342609
2017 LSD1-Mediated Epigenetic Reprogramming Drives CENPE Expression and Prostate Cancer Progression. Cancer research 83 28916652
2014 Mutations in CENPE define a novel kinetochore-centromeric mechanism for microcephalic primordial dwarfism. Human genetics 78 24748105
1996 The kinesin-like protein CENP-E is kinetochore-associated throughout poleward chromosome segregation during anaphase-A. Journal of cell science 75 8743943
1999 Cleavage furrows formed between centrosomes lacking an intervening spindle and chromosomes contain microtubule bundles, INCENP, and CHO1 but not CENP-E. Molecular biology of the cell 71 9950678
2011 First-time-in-human study of GSK923295, a novel antimitotic inhibitor of centromere-associated protein E (CENP-E), in patients with refractory cancer. Cancer chemotherapy and pharmacology 70 22020315
2018 The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases. The Journal of biological chemistry 69 29748388
2007 Farnesyl transferase inhibitors impair chromosomal maintenance in cell lines and human tumors by compromising CENP-E and CENP-F function. Molecular cancer therapeutics 62 17431110
2012 Evolutionarily conserved protein ERH controls CENP-E mRNA splicing and is required for the survival of KRAS mutant cancer cells. Proceedings of the National Academy of Sciences of the United States of America 60 23236152
1997 CENP-E is an essential kinetochore motor in maturing oocytes and is masked during mos-dependent, cell cycle arrest at metaphase II. Proceedings of the National Academy of Sciences of the United States of America 60 9256453
2012 Computational screening and molecular dynamics simulation of disease associated nsSNPs in CENP-E. Mutation research 57 22974711
2010 Mitotic kinesin CENP-E promotes microtubule plus-end elongation. Current biology : CB 57 20797864
2019 BubR1 phosphorylates CENP-E as a switch enabling the transition from lateral association to end-on capture of spindle microtubules. Cell research 56 31201382
2019 CENPE promotes lung adenocarcinoma proliferation and is directly regulated by FOXM1. International journal of oncology 55 31115500
2014 Chemogenetic evaluation of the mitotic kinesin CENP-E reveals a critical role in triple-negative breast cancer. Molecular cancer therapeutics 54 24928852
2008 The mitotic kinesin CENP-E is a processive transport motor. Proceedings of the National Academy of Sciences of the United States of America 53 18427114
2020 Leaving no-one behind: how CENP-E facilitates chromosome alignment. Essays in biochemistry 51 32347304
1996 Modulation of CENP-E organization at kinetochores by spindle microtubule attachment. Cell motility and the cytoskeleton 51 8894282
2004 Crystal structure of the motor domain of the human kinetochore protein CENP-E. Journal of molecular biology 47 15236970
2015 Cenp-E inhibitor GSK923295: Novel synthetic route and use as a tool to generate aneuploidy. Oncotarget 46 26320186
2015 Chromokinesin Kid and kinetochore kinesin CENP-E differentially support chromosome congression without end-on attachment to microtubules. Nature communications 45 25743205
2020 Photoswitchable CENP-E Inhibitor Enabling the Dynamic Control of Chromosome Movement and Mitotic Progression. Journal of the American Chemical Society 43 31927956
2011 CENP-E kinesin interacts with SKAP protein to orchestrate accurate chromosome segregation in mitosis. The Journal of biological chemistry 42 22110139
2001 Purification and characterization of native conventional kinesin, HSET, and CENP-E from mitotic hela cells. The Journal of biological chemistry 41 11382767
2009 Defects in chromosome congression and mitotic progression in KIF18A-deficient cells are partly mediated through impaired functions of CENP-E. Cell cycle (Georgetown, Tex.) 40 19625775
2010 Germinal Cell Aplasia in Kif18a Mutant Male Mice Due to Impaired Chromosome Congression and Dysregulated BubR1 and CENP-E. Genes & cancer 39 20981276
2016 SUMOylated NKAP is essential for chromosome alignment by anchoring CENP-E to kinetochores. Nature communications 38 27694884
2014 Kinetochore-microtubule attachment throughout mitosis potentiated by the elongated stalk of the kinetochore kinesin CENP-E. Molecular biology of the cell 35 24920822
2011 Initial testing of the CENP-E inhibitor GSK923295A by the pediatric preclinical testing program. Pediatric blood & cancer 35 21584937
1996 The centromere kinesin-like protein, CENP-E. An autoantigen in systemic sclerosis. Arthritis and rheumatism 34 8702444
2000 Specific regulation of CENP-E and kinetochores during meiosis I/meiosis II transition in pig oocytes. Molecular reproduction and development 32 10737967
2016 XAB2 functions in mitotic cell cycle progression via transcriptional regulation of CENPE. Cell death & disease 31 27735937
2015 TRAMM/TrappC12 plays a role in chromosome congression, kinetochore stability, and CENP-E recruitment. The Journal of cell biology 31 25918224
1994 Chromosomal localization of the genes encoding the kinetochore proteins CENPE and CENPF to human chromosomes 4q24-->q25 and 1q32-->q41, respectively, by fluorescence in situ hybridization. Genomics 30 7851898
2015 CTCF Recruits Centromeric Protein CENP-E to the Pericentromeric/Centromeric Regions of Chromosomes through Unusual CTCF-Binding Sites. Cell reports 29 26321640
2015 A Novel Time-Dependent CENP-E Inhibitor with Potent Antitumor Activity. PloS one 29 26649895
2002 Expression and behaviour of CENP-E at kinetochores during mouse spermatogenesis. Chromosoma 29 12068923
2007 BubR1 and CENP-E have antagonistic effects upon the stability of microtubule-kinetochore attachments in Drosophila S2 cell mitosis. Cell cycle (Georgetown, Tex.) 28 17525528
1998 Immunolocalization of alpha-tubulin, gamma-tubulin, and CENP-E in male rat and male mouse meiotic divisions: pathway of meiosis I spindle formation in mammalian spermatocytes. Developmental biology 28 9520321
2018 CENP-E as a target for cancer therapy: Where are we now? Life sciences 27 30031812
2009 Reduced expression of cenp-e in human hepatocellular carcinoma. Journal of experimental & clinical cancer research : CR 27 20021663
2006 Interaction of Skp1 with CENP-E at the midbody is essential for cytokinesis. Biochemical and biophysical research communications 27 16682006
2019 LUBAC controls chromosome alignment by targeting CENP-E to attached kinetochores. Nature communications 26 30655516
2013 The microtubule binding properties of CENP-E's C-terminus and CENP-F. Journal of molecular biology 25 23892111
2020 Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly. Journal of molecular cell biology 24 31174204
2020 Kinesin Family Member C1 (KIFC1) Regulated by Centrosome Protein E (CENPE) Promotes Proliferation, Migration, and Epithelial-Mesenchymal Transition of Ovarian Cancer. Medical science monitor : international medical journal of experimental and clinical research 24 33361741
2001 Elevating the level of Cdc34/Ubc3 ubiquitin-conjugating enzyme in mitosis inhibits association of CENP-E with kinetochores and blocks the metaphase alignment of chromosomes. The Journal of cell biology 24 11514588
2023 CENP-E activation by Aurora A and B controls kinetochore fibrous corona disassembly. Nature communications 23 37658044
2023 RZZ-Spindly and CENP-E form an integrated platform to recruit dynein to the kinetochore corona. The EMBO journal 23 37984321
2019 Impaired CENP-E Function Renders Large Chromosomes More Vulnerable to Congression Failure. Biomolecules 23 30691136
2019 Reduced expression of CENP-E contributes to the development of hepatocellular carcinoma and is associated with adverse clinical features. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 23 31881483
2009 The ATPase cycle of the mitotic motor CENP-E. The Journal of biological chemistry 23 19759394
2015 Synthetic Studies on Centromere-Associated Protein-E (CENP-E) Inhibitors: 2. Application of Electrostatic Potential Map (EPM) and Structure-Based Modeling to Imidazo[1,2-a]pyridine Derivatives as Anti-Tumor Agents. Journal of medicinal chemistry 22 26372373
2019 Mechanisms of kinesin-7 CENP-E in kinetochore-microtubule capture and chromosome alignment during cell division. Biology of the cell 21 30784092
2023 HPV16 E6 induces chromosomal instability due to polar chromosomes caused by E6AP-dependent degradation of the mitotic kinesin CENP-E. Proceedings of the National Academy of Sciences of the United States of America 20 36989302
2012 Microtubule capture by mitotic kinesin centromere protein E (CENP-E). The Journal of biological chemistry 20 22637578
2014 Tumor suppressor roles of CENP-E and Nsl1 in Drosophila epithelial tissues. Cell cycle (Georgetown, Tex.) 19 24626182
2011 UA62784 Is a cytotoxic inhibitor of microtubules, not CENP-E. Chemistry & biology 19 21609844
2021 CENPE Inhibition Leads to Mitotic Catastrophe and DNA Damage in Medulloblastoma Cells. Cancers 18 33804489
2023 ATAD2 is a driver and a therapeutic target in ovarian cancer that functions by upregulating CENPE. Cell death & disease 17 37479754
1999 Kinesin-like protein CENP-E is upregulated in rheumatoid synovial fibroblasts. Arthritis research 17 11056662
2020 Kinesin-7 CENP-E regulates chromosome alignment and genome stability of spermatogenic cells. Cell death discovery 16 32351712
2010 Aurora B kinase cooperates with CENP-E to promote timely anaphase onset. Chromosoma 16 20354862
2010 The interaction between mitotic checkpoint proteins, CENP-E and BubR1, is diminished in epothilone B-resistant A549 cells. Cell cycle (Georgetown, Tex.) 15 20237434
1998 Variegated aneuploidy in two siblings: phenotype, genotype, CENP-E analysis, and literature review. American journal of medical genetics 15 9450856
2021 Lin28A/CENPE Promoting the Proliferation and Chemoresistance of Acute Myeloid Leukemia. Frontiers in oncology 13 34868981
2018 Probing Mitotic CENP-E Kinesin with the Tethered Cargo Motion Assay and Laser Tweezers. Biophysical journal 13 29874614
2013 Synthetic studies of centromere-associated protein-E (CENP-E) inhibitors: 1.Exploration of fused bicyclic core scaffolds using electrostatic potential map. Bioorganic & medicinal chemistry 13 23816042
2021 Poly-SUMO-2/3 chain modification of Nuf2 facilitates CENP-E kinetochore localization and chromosome congression during mitosis. Cell cycle (Georgetown, Tex.) 12 33910471
2020 Distinct Mechanisms of Resistance to a CENP-E Inhibitor Emerge in Near-Haploid and Diploid Cancer Cells. Cell chemical biology 12 32442423

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