Affinage

NUF2

Kinetochore protein Nuf2 · UniProt Q9BZD4

Length
464 aa
Mass
54.3 kDa
Annotated
2026-06-10
63 papers in source corpus 28 papers cited in narrative 28 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NUF2 is an evolutionarily conserved kinetochore protein that forms the structural core required for chromosome segregation, originally identified as a spindle pole body-associated protein essential for nuclear division in budding yeast (PMID:8188751) and shown across fission yeast and vertebrates to be required for centromere-spindle connection and spindle assembly checkpoint signaling (PMID:11685532, PMID:12829748). It heterodimerizes stably with Hec1/NDC80 throughout the cell cycle—an interaction built on three buried Hec1 glutamate residues that form complementary ionic pairs with three Nuf2 lysines, such that disruption of either set destabilizes the NDC80 complex and blocks mitosis (PMID:24129578)—and localizes to the outer plate of the kinetochore where it constitutes a stable structural element distinct from dynamic checkpoint and motor proteins (PMID:15548592). NUF2/Hec1 are required to maintain kinetochore-microtubule attachment and to retain Mad1/Mad2 at kinetochores against microtubule-dependent stripping (PMID:14654001). The NUF2 calponin homology domain is dispensable for initial attachment but required to generate interkinetochore tension (PMID:21270439), and at the single-molecule level acts as a force-dependent friction regulator governing the slip-clutch behavior of NDC80 sliding along microtubules [PMID:bio_10.1101_2025.03.13.643154]. NUF2 also recruits CENP-E to kinetochores, both through a direct C-terminal interaction and through poly-SUMO-2/3 chain modification that enhances binding to the CENP-E SUMO-interacting motif (PMID:17535814, PMID:33910471). Beyond mitosis, NUF2 (with other NDC80 subunits) is required for pre-rRNA transcription and suppression of nucleolar stress during interphase (PMID:39705402). A de novo missense variant in the CH domain destabilizes the NUF2-NDC80 complex and causes a developmental syndrome with microcephaly and short stature (PMID:33721060). In cancer, NUF2 is transcriptionally activated by FOXM1 and YY1 and engages proliferative and survival signaling through interactions with multiple partners (PMID:35701983, PMID:40187571).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1994 High

    Established NUF2 as a conserved spindle pole body-associated protein required for nuclear division, framing it as a mitotic apparatus component rather than a soluble factor.

    Evidence Coimmunofluorescence with SPB markers, temperature-sensitive mutagenesis and biochemical fractionation in budding yeast

    PMID:8188751

    Open questions at the time
    • Molecular partners at the SPB undefined
    • No direct microtubule or centromere mechanism established
  2. 2001 High

    Distinguished NUF2's two functions—centromere-microtubule attachment and checkpoint signaling—by showing null and ts alleles separate spindle elongation from chromosome segregation and Mad2-dependent arrest.

    Evidence Gene disruption, ts alleles, and mad2 epistasis with live imaging in fission yeast

    PMID:11685532

    Open questions at the time
    • Biochemical basis of attachment vs. checkpoint roles unresolved
    • Vertebrate generality not yet shown
  3. 2003 High

    Defined the stable Nuf2-Hec1 complex and its outer-kinetochore requirement for retaining checkpoint proteins, showing NUF2 prevents microtubule-dependent stripping of Mad1/Mad2.

    Evidence GFP live imaging, conditional knockout and FRAP in DT40 cells; RNAi with spindle-depolymerization rescue in HeLa

    PMID:12829748 PMID:14654001

    Open questions at the time
    • Direct microtubule-binding mechanism not yet mapped
    • Stoichiometry of the full NDC80 complex not addressed here
  4. 2004 High

    Localized NUF2/Hec1 to the kinetochore outer plate as a stable structural core required for outer-plate formation and microtubule attachment, separating it from dynamic corona components.

    Evidence Light and electron microscopy with quantitative RNAi analysis in vertebrate cells

    PMID:15548592

    Open questions at the time
    • Atomic structure of the attachment interface unknown
    • How force is transmitted through the plate undefined
  5. 2005 High

    Showed the Nuf2-Ndc80 complex physically connects centromeres to the SPB and links it to inner kinetochore via CENP-H, establishing NUF2 as a bridge across the kinetochore.

    Evidence Meiotic live imaging with genetic epistasis in fission yeast; yeast two-hybrid and Co-IP with CENP-H in DT40

    PMID:15713649 PMID:15728720

    Open questions at the time
    • Direct vs. indirect nature of the CENP-H linkage in vertebrates not fully resolved
    • Regulation of complex removal by pheromone signaling mechanistically incomplete
  6. 2007 High

    Identified NUF2 as required for CENP-E kinetochore localization and tension generation through a direct C-terminal interaction, connecting the structural core to a kinetochore motor.

    Evidence GST pulldown, yeast two-hybrid, siRNA, and tension measurement in human cells

    PMID:17535814

    Open questions at the time
    • Whether interaction is direct in cells or scaffold-mediated not fully resolved
    • Regulation of the interaction unknown at this stage
  7. 2011 High

    Dissected the NUF2 calponin homology domain function, showing it is dispensable for initial attachment but required for full interkinetochore tension—separating attachment stability from force generation.

    Evidence siRNA-and-rescue structure-function mutagenesis with live imaging and interkinetochore distance assays

    PMID:21270439

    Open questions at the time
    • Biophysical mechanism of tension generation not addressed
    • How the CH domain engages microtubules unresolved
  8. 2013 High

    Defined the structural basis of Hec1-Nuf2 heterodimerization at residue resolution, identifying three ionic pairs whose disruption destabilizes the NDC80 complex and blocks mitosis.

    Evidence Co-IP, domain mapping and alanine-scanning mutagenesis with cell-based mitotic assays

    PMID:24129578

    Open questions at the time
    • No crystal structure of the full dimer reported here
    • Effects on downstream Spc24/Spc25 assembly not detailed
  9. 2015 Medium

    Extended NUF2 function to meiosis and identified its transcriptional control, showing precise dosage is required for oocyte chromosome alignment and that hnRNP K directly transactivates NUF2.

    Evidence mRNA injection, morpholino knockdown and immunofluorescence in mouse oocytes; ChIP and reporter assay with siRNA in cells

    PMID:25701787 PMID:26054848

    Open questions at the time
    • Meiosis-specific regulators of NUF2 undefined
    • hnRNP K regulation tested in a single lineage
  10. 2021 High

    Revealed a post-translational mechanism for CENP-E recruitment—mitotic poly-SUMO-2/3 chain modification of NUF2 enhances binding to the CENP-E SIM in a chain-length- and SUMO-type-specific manner.

    Evidence SUMO modification assays, Nuf2-SUMO/Ubc9 fusion rescue with SIM-mutant controls and binding affinity assays

    PMID:33910471

    Open questions at the time
    • SUMO ligase and acceptor lysines on NUF2 not pinpointed
    • Temporal dynamics of de-SUMOylation undefined
  11. 2021 Medium

    Linked NUF2 to human disease, showing a de novo CH-domain missense variant destabilizes the NUF2-NDC80 complex and produces aneuploidy and a developmental syndrome.

    Evidence Whole-exome sequencing with patient lymphoblastoid cell Western blot and chromosome/spindle analysis

    PMID:33721060

    Open questions at the time
    • Single case limits genotype-phenotype generality
    • Tissue-specific basis of microcephaly phenotype unexplained
  12. 2024 Medium

    Uncovered an interphase, non-mitotic role: NUF2 and other NDC80 subunits are required for pre-rRNA transcription and suppression of nucleolar stress.

    Evidence siRNA validation, pre-rRNA assays, POLR1A Western blot and RPL5 co-depletion epistasis in MCF10A cells

    PMID:39705402

    Open questions at the time
    • Molecular link between NUF2 and Pol I machinery unknown
    • Whether this requires the NDC80 complex or a separate NUF2 pool unresolved
  13. 2024 Medium

    Mapped NUF2 into oncogenic signaling, showing it is transcriptionally activated by FOXM1/YY1 and stabilizes or engages multiple partners to drive proliferation and survival across cancer types.

    Evidence ChIP/reporter assays, Co-IP, ubiquitination assays and pathway Western blots with rescue across glioma, ovarian, cholangiocarcinoma, HCC, LUAD, thyroid, gastric and prostate models

    PMID:35701983 PMID:36670423 PMID:37056930 PMID:38507923 PMID:39193700 PMID:39242581 PMID:40187571 PMID:40582584 PMID:41229848 PMID:41943078

    Open questions at the time
    • Mechanistic relationship between NUF2's kinetochore role and signaling functions unclear
    • Most interactions validated in single labs without reciprocal/structural confirmation
    • Whether signaling is direct or a consequence of proliferation not always separable
  14. 2025 Medium

    Provided the biophysical basis of the NUF2 CH domain's tension role, showing it acts as a force-dependent friction regulator enabling NDC80 slip-clutch microtubule end-coupling.

    Evidence Ultrafast force-clamp single-molecule spectroscopy on reconstituted Ndc80 complexes with domain mutants (preprint)

    PMID:bio_10.1101_2025.03.13.643154

    Open questions at the time
    • Preprint, not yet peer-reviewed
    • Behavior in the assembled kinetochore in cells not demonstrated

Open questions

Synthesis pass · forward-looking unresolved questions
  • How NUF2's canonical kinetochore/microtubule function mechanistically connects to its interphase nucleolar role and its reported cancer signaling activities remains unresolved.
  • No unifying model linking mitotic, nucleolar, and signaling functions
  • Direct vs. indirect nature of many cancer-context interactions undefined
  • No high-resolution structure of the human NDC80 complex on microtubules in the timeline

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 2 GO:0008092 cytoskeletal protein binding 2 GO:0060090 molecular adaptor activity 2
Localization
GO:0005694 chromosome 2 GO:0005815 microtubule organizing center 2 GO:0005730 nucleolus 1
Pathway
R-HSA-162582 Signal Transduction 3 R-HSA-1640170 Cell Cycle 3 R-HSA-8953854 Metabolism of RNA 1
Partners
BUB1BBUB3CENP-ECENP-HERBB3HNRNPA2B1NDC80USP7
Complex memberships
NDC80 complexkinetochore

Evidence

Reading pass · 28 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1994 NUF2 encodes a spindle pole body (SPB)-associated protein in budding yeast required for nuclear division; temperature-sensitive alleles cause arrest with a short mitotic spindle and undivided nucleus, and anti-Nuf2 antibodies cross-react with a mammalian centrosome-associated protein, suggesting conserved function. Coimmunofluorescence with SPB markers, temperature-sensitive mutagenesis, fractionation (salt/detergent/urea extraction) The Journal of cell biology High 8188751
2001 Nuf2 in fission yeast (S. pombe) is required for centromere-spindle connection and for the spindle assembly checkpoint; nuf2 null cells show spindle elongation without chromosome segregation, while certain ts alleles cause Mad2-dependent metaphase arrest, demonstrating Nuf2 is needed for both microtubule attachment and checkpoint signaling at the centromere. Gene disruption, temperature-sensitive allele analysis, epistasis with mad2 deletion, live cell microscopy Chromosoma High 11685532
2003 Nuf2 forms a stable complex with Hec1 throughout the cell cycle in vertebrate (chicken DT40) cells, localizing to centrosomes during G1/S and relocating to centromeres in G2/mitosis; conditional loss of either subunit causes prometaphase arrest, abolishes Mad2 kinetochore localization, and FRAP shows the complex is stably associated with centromeres during mitosis while its centrosome interaction is dynamic. GFP fusion live imaging, conditional knockout (DT40), FRAP, immunofluorescence Journal of cell science High 12829748
2003 RNAi depletion of Nuf2 or Hec1 in HeLa cells reduces both proteins at kinetochores and causes progressive, microtubule-dependent depletion of Mad1 and Mad2 from kinetochores during prometaphase; spindle depolymerization restores Mad1/Mad2 levels, supporting a model where Nuf2/Hec1 prevent microtubule-dependent stripping of checkpoint proteins. RNAi (siRNA) knockdown, quantitative immunofluorescence, spindle depolymerization rescue experiment Current biology : CB High 14654001
2004 Nuf2 and Hec1 localize throughout the outer plate (not the corona) of the vertebrate kinetochore, form a stable structural core distinct from dynamic checkpoint/motor proteins, and are required for formation and maintenance of the outer plate structure and for normal kinetochore microtubule attachment. Fluorescence light microscopy, live cell imaging, electron microscopy, RNAi knockdown with quantitative EM analysis Molecular biology of the cell High 15548592
2005 The Nuf2-Ndc80 complex (including Nuf2, Ndc80, Spc24, Spc25) disappears from centromeres during meiotic prophase in fission yeast coinciding with centromere-SPB dissociation; inactivation of Nuf2 by mutation causes premature centromere-SPB release, demonstrating that the Nuf2-Ndc80 complex physically connects centromeres to the SPB and that mating pheromone signaling triggers complex removal for meiotic kinetochore remodeling. Live cell imaging of GFP-tagged proteins in meiotic cells, genetic loss-of-function (nuf2 temperature-sensitive mutation), epistasis with pat1 mutant Molecular biology of the cell High 15728720
2005 The functional region of CENP-H interacts directly with Hec1 (a member of the Nuf2 complex) as demonstrated by yeast two-hybrid and coimmunoprecipitation in chicken DT40 cells; the Nuf2 complex is proposed to act as a connector between inner (CENP-H side) and outer kinetochore during mitosis. Yeast two-hybrid, coimmunoprecipitation, conditional loss-of-function mutant, FRAP Molecular and cellular biology High 15713649
2006 CDCA1 (NUF2) forms a complex with KNTC2 (Hec1/NDC80); a cell-permeable 19-amino-acid peptide derived from the CDCA1 binding domain to KNTC2 (residues 398–416) disrupts complex formation and suppresses lung cancer cell growth, mapping the interaction interface of the CDCA1-KNTC2 dimer. Cell-permeable peptide competition assay, siRNA knockdown, cell growth assays Cancer research Medium 17079454
2007 Human NUF2 is required for stable kinetochore localization of CENP-E; NUF2 physically interacts with the C-terminal domain of CENP-E via its own C-terminal domain (mapped by GST pulldown and yeast two-hybrid); siRNA depletion of NUF2 abolishes CENP-E kinetochore localization and reduces kinetochore tension, with double knockdown of CENP-E and NUF2 further eliminating tension. GST pulldown, yeast two-hybrid, siRNA knockdown, immunofluorescence, tension measurement The Journal of biological chemistry High 17535814
2011 The calponin homology (CH) domains of both Hec1 and Nuf2 contribute to kinetochore-microtubule attachment, but in distinct ways: Hec1 CH domain mutants show the most severe attachment defects; Nuf2 CH domain mutants generate stable kinetochore-microtubule attachments but fail to produce wild-type interkinetochore tension and delay anaphase onset, indicating the Nuf2 CH domain is dispensable for initial attachment but required for tension generation. Gene silencing and rescue (siRNA + re-expression of mutant constructs), live cell imaging, kinetochore-microtubule attachment assays, interkinetochore distance measurement Molecular biology of the cell High 21270439
2012 PTPIP51 interacts with Nuf2 (and CGI-99) in vitro and in vivo; the PTPIP51/Nuf2 complex localizes to the equatorial region during mitosis, as shown by Duolink proximity ligation assays and confocal microscopy. In vitro binding, in vivo colocalization, Duolink proximity ligation assay, confocal microscopy Biomolecules Low 24970130
2013 Hec1-Nuf2 heterodimerization requires three contiguous heptad repeats (Leu-324 to Leu-352) of Hec1; three buried glutamic acid residues (Glu-334, Glu-341, Glu-348) in Hec1 form complementary ionic pairs with three Nuf2 lysine residues (Lys-227, Lys-234, Lys-241); alanine substitution of either set disrupts the dimer, destabilizes the NDC80 complex, and blocks mitotic progression. Co-immunoprecipitation, domain mapping, alanine-scanning mutagenesis, cell-based mitotic progression assays The Journal of biological chemistry High 24129578
2015 Nuf2 localizes to kinetochores from germinal vesicle breakdown through metaphase I in mouse oocytes, disappears at anaphase I, and reappears at MII; overexpression causes defective spindles, chromosome misalignment, and spindle checkpoint activation blocking meiotic progression, while Nuf2 knockdown causes premature polar body extrusion with misaligned chromosomes and aneuploidy. mRNA injection, immunofluorescence, morpholino knockdown, flow cytometry for spindle checkpoint Cell cycle (Georgetown, Tex.) Medium 26054848
2015 hnRNP K directly transactivates the NUF2 gene; hnRNP K knockdown reduces NUF2 expression and causes failure in metaphase chromosome alignment, linking hnRNP K to kinetochore function via NUF2 transcriptional regulation. ChIP (chromatin immunoprecipitation), reporter assay, siRNA knockdown, immunofluorescence for chromosome alignment Biochemical and biophysical research communications Medium 25701787
2021 Nuf2 is preferentially modified by poly-SUMO-2/3 chains during mitosis; this modification is required for CENP-E kinetochore localization and chromosome congression. A Nuf2-Ubc9 fusion (to stimulate SUMO modification) or a Nuf2–SUMO-2 trimer fusion rescues CENP-E localization defects caused by global sumoylation inhibition, while Nuf2 fused to SUMO-2 monomer, dimer, or SUMO-1 trimer cannot. The SUMO-2/3-trimeric chain-modified Nuf2 binds CENP-E with higher affinity through CENP-E's SUMO-interacting motif (SIM). SUMO modification assays, Nuf2-SUMO/Ubc9 fusion rescue experiments, binding affinity assays (CENP-E SIM mutant controls), immunofluorescence Cell cycle (Georgetown, Tex.) High 33910471
2021 A de novo missense variant in NUF2 (affecting the calponin homology domain at the N-terminus, which interfaces with NDC80) causes markedly reduced protein levels of both NUF2 and NDC80, aneuploidy, increased micronuclei, and spindle abnormalities in patient-derived lymphoblastoid cells, demonstrating that NUF2 CH domain integrity is required for NDC80 complex stability in human cells and that NUF2 loss-of-function causes a developmental syndrome (microcephaly, short stature). Whole-exome sequencing, patient-derived lymphoblastoid cell analysis, Western blot for NUF2 and NDC80 levels, chromosome and spindle analysis Human genetics Medium 33721060
2022 FOXM1 transcriptionally activates NUF2 by binding to its promoter (mapped by luciferase reporter assays and ChIP); FOXM1-induced proliferation of glioma TMZ-resistant cells is dependent on elevated NUF2 expression, as NUF2 knockdown abolishes the FOXM1-mediated proliferative phenotype. Luciferase reporter assay, chromatin immunoprecipitation (ChIP), siRNA knockdown, rescue experiments Neuropathology Medium 35701983
2023 NUF2 interacts with HNRNPA2B1 (identified by immunoprecipitation/mass spectrometry) and activates the PI3K/AKT/mTOR signaling pathway in ovarian cancer cells; HNRNPA2B1 silencing reduces NUF2 expression, placing HNRNPA2B1 upstream of NUF2. Immunoprecipitation/mass spectrometry, Co-IP, siRNA knockdown, Western blot for PI3K/AKT/mTOR pathway components Journal of ovarian research Medium 36670423
2023 NUF2 promotes cholangiocarcinoma progression through the p38/MAPK signaling pathway by inhibiting p62-mediated autophagic degradation of transferrin receptor 1 (TFR1); NUF2 knockdown reduces TFR1 protein levels and impairs p38/MAPK activation. siRNA knockdown, Western blot, autophagy assays, Co-IP for p62-TFR1 interaction, xenograft models International journal of biological sciences Medium 37056930
2024 NUF2 interacts with ERBB3 in hepatocellular carcinoma cells and inhibits ERBB3 ubiquitination-mediated degradation, thereby stabilizing ERBB3 protein levels and activating the PI3K/AKT signaling pathway to regulate cell cycle progression. Co-immunoprecipitation, ubiquitination assay, Western blot, siRNA knockdown, xenograft Translational oncology Medium 38507923
2024 NUF2 interacts with BUB3 (confirmed by Co-IP and mass spectrometry) and stabilizes it by preventing proteasomal degradation; NUF2 activates NF-κB signaling in a BUB3-dependent manner to promote lung adenocarcinoma progression. Co-immunoprecipitation, mass spectrometry, Western blot for BUB3 stability, transcriptomic sequencing, NF-κB pathway analysis, xenograft World journal of surgical oncology Medium 41943078
2024 NUF2 depletion epigenetically inhibits expression of magnesium transporters by reducing H3K4me3 at their promoters, thereby lowering intracellular Mg2+ concentration and suppressing PI3K/Akt/mTOR-mediated protein synthesis in anaplastic thyroid cancer cells. siRNA knockdown, ChIP for H3K4me3, intracellular Mg2+ measurement, PI3K/Akt/mTOR pathway Western blot, xenograft Cell death & disease Medium 39242581
2024 NUF2 promotes gastric cancer G2/M phase transition and inhibits apoptosis via the MAPK pathway; quercetin was identified as a selective NUF2 inhibitor by virtual docking and microscale thermophoresis, suppressing tumor growth in CDX, organoid, and PDX models. siRNA knockdown, live-cell time-lapse imaging, transcriptomics, flow cytometry, virtual docking, microscale thermophoresis, CDX/organoid/PDX models Chinese medical journal Medium 39193700
2024 NUF2 is required for pre-rRNA transcription (the primary rate-limiting step of ribosome biogenesis) in human breast epithelial cells (MCF10A); siRNA depletion of NUF2 (and other NDC80 complex members NDC80, SPC24, SPC25) reduces levels of RNA polymerase I largest subunit POLR1A and activates the nucleolar stress pathway (elevated TP53 and p21 mRNAs, reversible by RPL5 co-depletion). Genome-wide siRNA screen validation, pre-rRNA transcription assays, Western blot for POLR1A, qRT-PCR for TP53/CDKN1A, RPL5 co-depletion epistasis Molecular biology of the cell Medium 39705402
2025 Single Ndc80 complexes undergo processive, bi-directional sliding along microtubule walls; plus-end-directed forces induce a mobile catch-bond while forces away from the plus-end trigger slip-bond behavior; this dual behavior arises from force-dependent modulation of the Nuf2 calponin homology domain's microtubule binding, identifying Nuf2 as a friction regulator that enables the kinetochore's slip-clutch end-coupling mechanism. Ultrafast force-clamp spectroscopy (single-molecule), reconstituted Ndc80 complexes, domain-specific mutational analysis bioRxivpreprint Medium bio_10.1101_2025.03.13.643154
2025 YY1 directly transcriptionally activates NUF2 by binding its promoter; NUF2 promotes prostate cancer progression by recruiting p38 and accelerating its phosphorylation, activating the p38/MAPK signaling pathway; fisetin was identified as a small-molecule NUF2 inhibitor that reverses NUF2-driven proliferation. ChIP, luciferase reporter assay, Co-immunoprecipitation for NUF2-p38, phospho-p38 Western blot, siRNA/overexpression rescue, in vivo xenograft Biochemical pharmacology Medium 40187571
2025 NUF2 directly binds BUB1B (confirmed by co-immunoprecipitation) and promotes LUAD progression via BUB1B-mediated mTORC1 pathway activation (evidenced by phosphorylation of 4EBP1 and S6); BUB1B overexpression rescues anti-tumor effects of NUF2 knockdown. Co-immunoprecipitation, Western blot for mTORC1 substrates, siRNA knockdown and rescue, xenograft Journal of thoracic disease Medium 41229848
2025 USP7 stabilizes NUF2 protein by mediating its deubiquitination (confirmed by immunoprecipitation and ubiquitination assay); NUF2 positively regulates SLC7A11 expression to suppress ferroptosis and promote ovarian cancer progression; USP7 depletion effects on cancer cells are mediated through NUF2 downregulation. Co-immunoprecipitation, ubiquitination assay, siRNA knockdown epistasis, ferroptosis markers (Fe2+, ROS, MDA), xenograft Experimental cell research Medium 40582584

Source papers

Stage 0 corpus · 63 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2004 Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites. Molecular biology of the cell 215 15548592
2003 Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores. Current biology : CB 139 14654001
2003 Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells. Journal of cell science 131 12829748
2006 Activation of CDCA1-KNTC2, members of centromere protein complex, involved in pulmonary carcinogenesis. Cancer research 116 17079454
2001 A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link between the kinetochore function and the spindle checkpoint. Chromosoma 111 11685532
2011 The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis. Molecular biology of the cell 105 21270439
2012 Structural and inhibition insights into carbonic anhydrase CDCA1 from the marine diatom Thalassiosira weissflogii. Biochimie 81 22381359
2007 Human NUF2 interacts with centromere-associated protein E and is essential for a stable spindle microtubule-kinetochore attachment. The Journal of biological chemistry 78 17535814
2009 siRNA-mediated knockdown against CDCA1 and KNTC2, both frequently overexpressed in colorectal and gastric cancers, suppresses cell proliferation and induces apoptosis. Biochemical and biophysical research communications 76 19878654
1994 Nuf2, a spindle pole body-associated protein required for nuclear division in yeast. The Journal of cell biology 64 8188751
2005 Dissociation of the Nuf2-Ndc80 complex releases centromeres from the spindle-pole body during meiotic prophase in fission yeast. Molecular biology of the cell 58 15728720
2021 LncRNA CDKN2B-AS1 stabilized by IGF2BP3 drives the malignancy of renal clear cell carcinoma through epigenetically activating NUF2 transcription. Cell death & disease 49 33608495
2015 siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo. Bioscience reports 47 25370920
2014 Silencing of NUF2 inhibits tumor growth and induces apoptosis in human hepatocellular carcinomas. Asian Pacific journal of cancer prevention : APJCP 43 25374179
2017 Phase I clinical trial of cell division associated 1 (CDCA1) peptide vaccination for castration resistant prostate cancer. Cancer science 42 28498618
2015 Downregulation of NUF2 inhibits tumor growth and induces apoptosis by regulating lncRNA AF339813. International journal of clinical and experimental pathology 41 26045769
2005 The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis. Molecular and cellular biology 39 15713649
2015 Cadmium-containing carbonic anhydrase CDCA1 in marine diatom Thalassiosira weissflogii. Marine drugs 35 25815892
2015 Heterogeneous nuclear ribonucleoprotein K upregulates the kinetochore complex component NUF2 and promotes the tumorigenicity of colon cancer cells. Biochemical and biophysical research communications 32 25701787
2016 Silencing of NUF2 inhibits proliferation of human osteosarcoma Saos-2 cells. European review for medical and pharmacological sciences 29 27049259
2015 Nuf2 is required for chromosome segregation during mouse oocyte meiotic maturation. Cell cycle (Georgetown, Tex.) 28 26054848
2008 Cancer-associated splicing variants of the CDCA1 and MSMB genes expressed in cancer cell lines and surgically resected gastric cancer tissues. Surgery 26 19081476
2023 NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell Indicator. International journal of molecular sciences 25 36835637
2020 Correlation of NUF2 Overexpression with Poorer Patient Survival in Multiple Cancers. Cancer research and treatment 25 33421974
2014 Identification of CDCA1-derived long peptides bearing both CD4+ and CD8+ T-cell epitopes: CDCA1-specific CD4+ T-cell immunity in cancer patients. International journal of cancer 23 24734272
2014 SiRNA-mediated knockdown against NUF2 suppresses tumor growth and induces cell apoptosis in human glioma cells. Cellular and molecular biology (Noisy-le-Grand, France) 22 25481014
2023 NUF2 Drives Cholangiocarcinoma Progression and Migration via Inhibiting Autophagic Degradation of TFR1. International journal of biological sciences 20 37056930
2022 NUF2 Drives Clear Cell Renal Cell Carcinoma by Activating HMGA2 Transcription through KDM2A-mediated H3K36me2 Demethylation. International journal of biological sciences 19 35813477
2022 NUF2 overexpression contributes to epithelial ovarian cancer progression via ERBB3-mediated PI3K-AKT and MAPK signaling axes. Frontiers in oncology 19 36620547
2023 NUF2 promotes tumorigenesis by interacting with HNRNPA2B1 via PI3K/AKT/mTOR pathway in ovarian cancer. Journal of ovarian research 18 36670423
2020 Identification of NUF2 and FAM83D as potential biomarkers in triple-negative breast cancer. PeerJ 18 33005492
2022 A noncoding regulatory RNA Gm31932 induces cell cycle arrest and differentiation in melanoma via the miR-344d-3-5p/Prc1 (and Nuf2) axis. Cell death & disease 15 35393397
2022 FOXM1-mediated NUF2 expression confers temozolomide resistance to human glioma cells by regulating autophagy via the PI3K/AKT/mTOR signaling pathway. Neuropathology : official journal of the Japanese Society of Neuropathology 15 35701983
2012 Interaction of PTPIP51 with Tubulin, CGI-99 and Nuf2 During Cell Cycle Progression. Biomolecules 14 24970130
2024 Targeting NUF2 suppresses gastric cancer progression through G2/M phase arrest and apoptosis induction. Chinese medical journal 13 39193700
2022 ANP32E contributes to gastric cancer progression via NUF2 upregulation. Molecular medicine reports 13 35795988
2021 Poly-SUMO-2/3 chain modification of Nuf2 facilitates CENP-E kinetochore localization and chromosome congression during mitosis. Cell cycle (Georgetown, Tex.) 12 33910471
2021 A missense variant in NUF2, a component of the kinetochore NDC80 complex, causes impaired chromosome segregation and aneuploidy associated with microcephaly and short stature. Human genetics 10 33721060
2021 Expression and clinical significance of NUF2 in kidney renal clear cell carcinoma. Translational andrology and urology 9 34733658
2024 NUF2 regulated the progression of hepatocellular carcinoma through modulating the PI3K/AKT pathway via stabilizing ERBB3. Translational oncology 8 38507923
2021 Evaluation of NUF2 and GMNN Expression in Prostate Cancer: Potential Biomarkers for Prostate Cancer Screening. Reports of biochemistry & molecular biology 7 34604412
2024 NUF2 Expression in Cancer Tissues and Lymph Nodes Suggests Post-Surgery Recurrence of Non-Small Cell Lung Cancer. Diagnostics (Basel, Switzerland) 5 38472943
2024 NUF2 Promotes Breast Cancer Metastasis via Activating Wnt/β-Catenin Pathways. Frontiers in bioscience (Landmark edition) 5 39614440
2024 NUF2 is associated with cancer stem cell characteristics and a potential drug target for prostate cancer. Frontiers in molecular biosciences 5 39703688
2025 Berberine restrains non-small cell lung cancer cell growth, invasion and glycolysis via inactivating the SPC25/NUF2 pathway. Naunyn-Schmiedeberg's archives of pharmacology 4 39755832
2024 Maintenance of magnesium homeostasis by NUF2 promotes protein synthesis and anaplastic thyroid cancer progression. Cell death & disease 4 39242581
2024 A role for the kinetochore protein, NUF2, in ribosome biogenesis. Molecular biology of the cell 4 39705402
2023 FOXM1 promotes neurofibromatosis type 1-associated malignant peripheral nerve sheath tumor progression in a NUF2-dependent manner. Cancer gene therapy 4 37488294
2022 De novo NUF2 variant in a novel inherited bone marrow failure syndrome including microcephaly and renal hypoplasia. British journal of haematology 4 36111525
2019 [Expression of NUF2 in breast cancer and its clinical significance]. Nan fang yi ke da xue xue bao = Journal of Southern Medical University 4 31140425
2025 Uncovering novel functions of NUF2 in glioblastoma and MRI-based expression prediction. Scientific reports 3 40890233
2025 NUF2 activated by YY1 promotes prostate cancer malignancy via p38/MAPK signaling axis and serves as a therapeutic target. Biochemical pharmacology 2 40187571
2025 NUF2 and NEK2 promote malignant progression of gallbladder cancer by remodeling the extracellular matrix. Carcinogenesis 2 40220284
2025 Immunohistochemical expression of POC1A, NUF2, and Ki-67 in invasive ductal carcinoma of the breast. American journal of clinical pathology 2 41237374
2022 Clinical significance and prospective molecular mechanism of NUF2 in gastric cancer exploration: A comprehensive study based on the GeneChip, GEO, Oncomine, and TCGA databases. Medicine 2 35777029
2013 Complementary interhelical interactions between three buried Glu-Lys pairs within three heptad repeats are essential for Hec1-Nuf2 heterodimerization and mitotic progression. The Journal of biological chemistry 2 24129578
2025 USP7-mediated NUF2 deubiquitination accelerates ovarian cancer progression through regulating SLC7A11 expression. Experimental cell research 1 40582584
2026 Clinical significance and immune effect of expression of NUF2 in lung adenocarcinoma. Medicine 0 41517679
2026 Anti-cancer effects of carvacrol on NUF2, CASP3 and CLDN6 expression in T47D breast cancer cells. Naunyn-Schmiedeberg's archives of pharmacology 0 41569314
2026 NUF2 interacts with BUB3 and drives lung adenocarcinoma progression through activation of the NF-κB pathway. World journal of surgical oncology 0 41943078
2025 Analysis of a cancer-associated mutation in the budding yeast Nuf2 kinetochore protein. microPublication biology 0 40161439
2025 NUF2 promotes the malignant development of lung adenocarcinoma through the mTORC1 signaling pathway via binding to BUB1B. Journal of thoracic disease 0 41229848
2025 A core stemness-associated module reveals PLK1, NUF2, KIF23, CDCA8, TOP2A, CENPF, AURKA, and ASPM as key genes in rectal cancer. European journal of medical research 0 41373021

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