Affinage

NUF2

Kinetochore protein Nuf2 · UniProt Q9BZD4

Length
464 aa
Mass
54.3 kDa
Annotated
2026-04-29
62 papers in source corpus 30 papers cited in narrative 29 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NUF2 is a core structural subunit of the NDC80 kinetochore complex that is essential for chromosome segregation during cell division and also contributes to interphase ribosome biogenesis. NUF2 heterodimerizes with NDC80/Hec1 through a coiled-coil interface stabilized by buried Glu-Lys pairs, and this dimer forms the outer plate of the kinetochore where it directly engages spindle microtubule walls via the calponin-homology (CH) domains of both subunits; the Nuf2 CH domain specifically generates interkinetochore tension and acts as a force-dependent friction regulator enabling bidirectional kinetochore sliding along microtubules (PMID:24129578, PMID:21270439, PMID:15548592). NUF2 recruits CENP-E to kinetochores through mitosis-specific poly-SUMO-2/3 modification that engages CENP-E's SUMO-interacting motif, retains spindle checkpoint proteins Mad1/Mad2 against microtubule-dependent stripping, and during interphase supports pre-rRNA transcription by maintaining RNA Pol I largest subunit levels (PMID:33910471, PMID:14654001, PMID:39705402). A de novo missense variant disrupting the NUF2 CH domain causes destabilization of the NDC80–NUF2 heterodimer, aneuploidy, and a human syndrome of microcephaly and short stature, establishing NUF2 as the first NDC80 complex member linked to a Mendelian disorder (PMID:33721060).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 1994 High

    Identification of Nuf2 as a conserved spindle pole body component required for nuclear division established a new gene essential for mitotic spindle function.

    Evidence Temperature-sensitive alleles in budding yeast causing arrested mitotic spindles; cross-reactive antibody staining of mammalian centrosomal protein

    PMID:8188751

    Open questions at the time
    • Molecular partners at the spindle pole body unknown
    • Mechanism of mitotic arrest uncharacterized
    • Whether Nuf2 directly contacts microtubules not tested
  2. 2001 High

    Demonstration that Nuf2 connects centromeres to spindle pole bodies and activates the Mad2-dependent spindle checkpoint established its dual role as a structural kinetochore component and checkpoint regulator.

    Evidence Fission yeast gene disruption and ts alleles with Mad2 epistasis; live-cell fluorescence microscopy

    PMID:11685532

    Open questions at the time
    • Whether Nuf2 acts alone or in a complex at the centromere was unknown
    • Direct physical interaction with checkpoint proteins not shown
  3. 2003 High

    Discovery that Nuf2 and Hec1 form a constitutive complex that relocalizes from centrosomes to kinetochores during the cell cycle, and that their loss abolishes Mad2 kinetochore localization, defined the Nuf2-Hec1 dimer as the functional unit linking outer kinetochore assembly to checkpoint signaling.

    Evidence Conditional knockouts in DT40 cells with FRAP, RNAi in HeLa with quantitative immunofluorescence and microtubule depolymerization rescue

    PMID:12829748 PMID:14654001

    Open questions at the time
    • Stoichiometry and additional subunits of the complex not yet identified
    • How Mad1/Mad2 stripping is prevented mechanistically unknown
  4. 2004 High

    Ultrastructural mapping of Nuf2-Hec1 to the kinetochore outer plate and demonstration that their depletion eliminates outer plate formation and poleward force generation defined these proteins as the structural core of the microtubule-binding interface.

    Evidence Quantitative electron microscopy, live-cell imaging, and RNAi in vertebrate cells

    PMID:15548592

    Open questions at the time
    • Direct microtubule binding by individual subunits not demonstrated
    • Connection to inner kinetochore components not mapped
  5. 2005 High

    Identification of CENP-H as a physical link between the inner kinetochore and the Nuf2 complex, and demonstration that meiotic signaling triggers Nuf2-Ndc80 complex removal from centromeres, placed the complex at the interface of kinetochore architecture and cell-cycle-dependent remodeling.

    Evidence Yeast two-hybrid and co-IP in DT40 cells for CENP-H interaction; live-cell imaging of conditional nuf2 mutants in fission yeast meiosis

    PMID:15713649 PMID:15728720

    Open questions at the time
    • Whether Nuf2 directly contacts CENP-H or acts through Hec1 unclear
    • Regulation mechanism for complex removal at meiotic prophase unknown
  6. 2007 High

    Mapping of the NUF2-CENP-E direct interaction and demonstration that NUF2 is required for CENP-E kinetochore recruitment and interkinetochore tension generation revealed NUF2's role as a platform for motor protein recruitment beyond structural microtubule attachment.

    Evidence GST pulldown, yeast two-hybrid, siRNA double knockdown with kinetochore tension measurements in HeLa cells

    PMID:17535814

    Open questions at the time
    • Post-translational modification driving CENP-E recruitment not identified
    • How CENP-E and Nuf2-Hec1 cooperate for tension generation mechanistically unclear
  7. 2011 High

    Structure-function dissection of Nuf2's calponin-homology domain showed it contributes specifically to interkinetochore tension rather than initial microtubule capture, distinguishing its role from the Hec1 CH domain.

    Evidence RNAi/rescue with CH domain point mutants in HeLa cells, quantitative tension and attachment measurements

    PMID:21270439

    Open questions at the time
    • Biophysical mechanism by which the Nuf2 CH domain generates tension not resolved
    • Whether CH domain contacts microtubule lattice or tubulin tails unknown
  8. 2013 High

    Identification of three buried Glu-Lys pairs in the Hec1-Nuf2 coiled-coil interface as the specificity determinants for heterodimerization provided the first atomic-resolution understanding of NDC80 complex assembly.

    Evidence Alanine-scanning mutagenesis with in vitro binding assays and co-IP; mitotic progression assays in cells

    PMID:24129578

    Open questions at the time
    • Full crystal structure of the human Hec1-Nuf2 coiled-coil interface not yet reported
    • How destabilization of these pairs affects downstream kinetochore architecture in vivo unclear
  9. 2015 High

    Demonstration that Nuf2 dosage is critical for meiotic progression in mouse oocytes — with both overexpression and depletion causing chromosome misalignment and aneuploidy — extended its essential role from mitosis to mammalian meiosis.

    Evidence mRNA injection/overexpression and morpholino knockdown in mouse oocytes with live imaging and flow cytometry

    PMID:26054848

    Open questions at the time
    • Whether meiotic phenotypes involve the same CH-domain and SUMO mechanisms as mitosis not tested
  10. 2021 High

    Two breakthroughs resolved key outstanding questions: poly-SUMO-2/3 modification of Nuf2 was identified as the molecular signal recruiting CENP-E to kinetochores via its SIM, and a patient-derived NUF2 CH domain variant was shown to destabilize the NDC80-NUF2 dimer causing microcephaly and aneuploidy — the first human disease linked to an NDC80 complex member.

    Evidence SUMO fusion rescue experiments with SIM-mutant controls; whole-exome sequencing with patient lymphoblastoid cell functional validation

    PMID:33721060 PMID:33910471

    Open questions at the time
    • SUMO E3 ligase modifying Nuf2 not identified
    • Whether additional patients carry NUF2 variants unknown
    • Genotype-phenotype relationship for partial versus complete loss of function not established
  11. 2024 High

    Discovery that NUF2 and its NDC80 complex partners support pre-rRNA transcription during interphase by maintaining RNA Pol I subunit levels revealed an unexpected non-mitotic function in ribosome biogenesis and nucleolar stress signaling.

    Evidence Genome-wide siRNA screen in interphase breast epithelial cells, RT-qPCR, nucleolar stress pathway epistasis with RPL5 co-depletion

    PMID:39705402

    Open questions at the time
    • Whether NUF2 localizes to the nucleolus during interphase not shown
    • Mechanism by which NUF2 maintains POLR1A levels unknown
    • Whether interphase function is independent of NDC80 complex formation not tested
  12. 2025 High

    Single-molecule biophysics identified the Nuf2 CH domain as a direction-dependent friction regulator on microtubules: plus-end-directed force produces catch-bond behavior while minus-end-directed force produces slip-bond behavior, providing the physical basis for slip-clutch kinetochore mechanics.

    Evidence Ultrafast force-clamp spectroscopy on single reconstituted Ndc80 complexes (preprint)

    PMID:bio_10.1101_2025.03.13.643154

    Open questions at the time
    • Preprint not yet peer-reviewed
    • Whether catch-bond behavior requires post-translational modifications in vivo untested
    • How Aurora B phosphorylation of the Hec1 tail modulates Nuf2 CH domain friction unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • Major open questions include the identity of the SUMO E3 ligase that modifies Nuf2, the structural basis for the Nuf2 CH domain's directional force asymmetry on the microtubule lattice, and the mechanism by which NUF2 supports RNA Pol I-mediated transcription during interphase.
  • SUMO E3 ligase targeting Nuf2 unidentified
  • No high-resolution structure of Nuf2 CH domain engaged with microtubule under force
  • Interphase nucleolar mechanism entirely uncharacterized

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 3 GO:0008092 cytoskeletal protein binding 2
Localization
GO:0005694 chromosome 5 GO:0005815 microtubule organizing center 2 GO:0005634 nucleus 1 GO:0005730 nucleolus 1
Pathway
R-HSA-1640170 Cell Cycle 7 R-HSA-392499 Metabolism of proteins 2 R-HSA-8953854 Metabolism of RNA 1
Partners
BUB1BCENP-ECENP-HMAPK14NDC80SPC25USP7
Complex memberships
NDC80 complex (NDC80/Hec1–NUF2–SPC24–SPC25)

Evidence

Reading pass · 29 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1994 Nuf2 (budding yeast) is a spindle pole body-associated protein required for nuclear division; temperature-sensitive alleles cause arrest with a shortened mitotic spindle, and anti-Nuf2 antibodies cross-react with a mammalian 73 kDa centrosomal protein, indicating conserved function. Coimmunofluorescence with SPB markers, temperature-sensitive allele analysis, fractionation (salt/detergent/urea extraction), cross-reactive antibody staining of mammalian cells The Journal of cell biology High 8188751
2001 Fission yeast Nuf2 is required at the centromere to establish a physical connection to the spindle pole body for proper chromosome segregation; nuf2 disruption causes spindle elongation without chromosome segregation, and certain nuf2 ts alleles trigger Mad2-dependent metaphase arrest, linking Nuf2 to the spindle checkpoint. Gene disruption, temperature-sensitive allele analysis, epistasis with mad2 mutant, live-cell fluorescence microscopy Chromosoma High 11685532
2003 In vertebrate (chicken DT40) cells, Nuf2 is physically associated with Hec1 throughout the cell cycle; the Nuf2-Hec1 complex localizes to centrosomes during G1/S and moves to the centromere in G2/mitosis. Conditional loss of Nuf2 or Hec1 causes prometaphase arrest; Mad2 localization to kinetochores is lost in mutant cells, whereas CENP-A, -C, -H and BubR1 remain. FRAP shows the complex stably associates with centromeres but dynamically with centrosomes. GFP fusion live imaging, FRAP, conditional loss-of-function (DT40 conditional knockouts), immunofluorescence Journal of cell science High 12829748
2003 RNAi depletion of Nuf2 or Hec1 in HeLa cells reduces both proteins at kinetochores and in the cytoplasm; Mad1 and Mad2 accumulate at kinetochores in early prometaphase but are depleted ~5-fold during the prometaphase block in a microtubule-dependent manner, indicating Nuf2/Hec1 prevent microtubule-dependent stripping of Mad1/Mad2 from unattached kinetochores. RNAi knockdown, quantitative immunofluorescence, spindle depolymerization rescue experiments Current biology : CB High 14654001
2004 Nuf2 and Hec1 localize throughout the outer plate (not corona) of the vertebrate kinetochore, form a stable 'core' structure distinct from dynamic spindle checkpoint and motor proteins, and are required for formation/maintenance of the outer plate itself and for normal kinetochore microtubule attachment and poleward force generation. Fluorescence light microscopy, live-cell imaging, quantitative electron microscopy, RNAi knockdown Molecular biology of the cell High 15548592
2005 CENP-H interacts with Hec1 (a member of the Nuf2 complex) by yeast two-hybrid, and the Nuf2 complex co-immunoprecipitates with CENP-H in chicken DT40 cells; FRAP shows both Hec1 and CENP-H form stable centromere associations during mitosis, supporting a model in which the Nuf2 complex acts as a connector between inner and outer kinetochores. Yeast two-hybrid, co-immunoprecipitation, FRAP, conditional loss-of-function in DT40 cells Molecular and cellular biology High 15713649
2005 In fission yeast, the Nuf2-Ndc80 complex (Nuf2, Ndc80, Spc24, Spc25) disappears from centromeres during meiotic prophase coinciding with centromere-SPB dissociation; inactivation of Nuf2 in pat1 mutant cells causes premature centromere-SPB release, demonstrating that the Nuf2-Ndc80 complex physically connects centromeres to the SPB and that mating pheromone signaling triggers its removal for meiotic kinetochore remodeling. Live-cell fluorescence microscopy, conditional nuf2 mutation in meiotic cells, genetic epistasis with pat1 mutant and pheromone signaling Molecular biology of the cell High 15728720
2006 CDCA1 (NUF2) and KNTC2 (Hec1/NDC80) form a binding complex; a cell-permeable 19-amino-acid CDCA1-derived peptide corresponding to the KNTC2-binding domain inhibits CDCA1-KNTC2 complex formation and suppresses lung cancer cell growth, mapping the interaction domain to the C-terminal region of CDCA1. Cell-permeable peptide inhibition, cell growth assays, domain mapping Cancer research Medium 17079454
2007 Human NUF2 is required for stable kinetochore localization of CENP-E; NUF2 directly binds the C-terminal domain of CENP-E, mapped by GST pulldown and yeast two-hybrid. SiRNA depletion of NUF2 abrogates CENP-E kinetochore localization and reduces kinetochore tension; double knockdown of CENP-E and NUF2 further abolishes tension, indicating NUF2 and CENP-E cooperate for stable microtubule-kinetochore attachment. GST pulldown, yeast two-hybrid, siRNA knockdown, immunofluorescence, kinetochore tension measurement The Journal of biological chemistry High 17535814
2011 The calponin homology (CH) domain of Nuf2 and the CH domain plus tail of Hec1 make distinct contributions to kinetochore-microtubule attachment: Hec1 CH domain mutations cause the most severe attachment defects, while Nuf2 CH domain mutations generate stable attachments but fail to produce wild-type interkinetochore tension and delay anaphase onset. Gene silencing and rescue with domain mutants in HeLa cells, kinetochore-microtubule attachment assays, interkinetochore tension measurements, live-cell imaging Molecular biology of the cell High 21270439
2012 PTPIP51 forms in vitro and in vivo complexes with Nuf2 and CGI-99; Duolink proximity ligation assays localize the PTPIP51/Nuf2 complex to the equatorial region during mitosis, and PTPIP51 associates with the spindle apparatus, suggesting a role in mitotic processes. Co-immunoprecipitation, Duolink proximity ligation assay, confocal microscopy Biomolecules Low 24970130
2013 Hec1-Nuf2 heterodimerization requires three contiguous heptad repeats (Leu-324 to Leu-352) of Hec1's coiled-coil domain; three buried Glu-Lys pairs (Glu-334/Lys-227, Glu-341/Lys-234, Glu-348/Lys-241) between Hec1 and Nuf2 provide the major specificity and stability for dimerization and NDC80 complex formation. Alanine substitutions of these residues diminish binding, compromise complex formation, and adversely affect mitotic progression. In vitro binding assays, alanine-scanning mutagenesis, co-immunoprecipitation, mitotic progression assays The Journal of biological chemistry High 24129578
2015 Nuf2 localizes to kinetochores from germinal vesicle breakdown to metaphase I in mouse oocytes, disappears at anaphase I, and reappears at MII. Overexpression causes defective spindles, misaligned chromosomes, and SAC activation, blocking anaphase onset; knockdown leads to precocious polar body extrusion, misaligned chromosomes, and aneuploidy, demonstrating Nuf2 is a critical regulator of meiotic cell cycle progression in oocytes. mRNA injection/overexpression, morpholino/antibody knockdown, immunofluorescence, live imaging, flow cytometry Cell cycle (Georgetown, Tex.) High 26054848
2015 hnRNP K directly transactivates the NUF2 gene promoter in HCT116 colon cancer cells; knockdown of both hnRNP K and NUF2 causes failure in metaphase chromosome alignment, establishing hnRNP K as an upstream transcriptional regulator of NUF2 mitotic function. Luciferase reporter assay, ChIP, siRNA knockdown, chromosome alignment assays Biochemical and biophysical research communications Medium 25701787
2021 A de novo missense variant in NUF2 at the calponin homology domain disrupts NUF2-NDC80 hydrophobic interactions, causing loss of stability of the NDC80-NUF2 heterodimer; patient-derived lymphoblastoid cells show markedly reduced NUF2 and NDC80 protein levels, aneuploidy, increased micronuclei, and spindle abnormalities, establishing NUF2 as the first NDC80 complex member associated with a human syndrome (microcephaly and short stature). Whole-exome sequencing, structural modeling, patient-derived cell line analysis (Western blot, karyotyping, immunofluorescence) Human genetics High 33721060
2021 Nuf2 is preferentially modified by poly-SUMO-2/3 chains during mitosis; this modification is upregulated at mitotic entry, temporally coinciding with CENP-E kinetochore localization. Expressing Nuf2-Ubc9 or Nuf2-SUMO-2 trimer fusion proteins rescues CENP-E kinetochore localization and chromosome congression defects caused by global sumoylation inhibition; the rescue requires a functional SUMO-interacting motif (SIM) in CENP-E, demonstrating that poly-SUMO-2/3 chains on Nuf2 directly recruit CENP-E to kinetochores. Sumoylation assays, Nuf2-Ubc9 and Nuf2-SUMO fusion rescue experiments, binding affinity assays, chromosome congression assays, SUMO inhibition Cell cycle (Georgetown, Tex.) High 33910471
2022 FOXM1 binds directly to the NUF2 promoter and transcriptionally activates NUF2 expression in glioma cells; FOXM1-driven MPNST/glioma cell proliferation is dependent on elevated NUF2, as NUF2 knockdown abolishes the FOXM1-induced proliferation. Luciferase reporter assay, chromatin immunoprecipitation (ChIP), siRNA knockdown, in vitro and in vivo proliferation assays Cancer gene therapy / Neuropathology Medium 35701983 37488294
2022 NUF2 epigenetically activates HMGA2 transcription in clear cell renal cell carcinoma by suppressing lysine demethylase 2A (KDM2A) expression, thereby reducing KDM2A occupancy on the HMGA2 promoter and increasing H3K36me2 methylation. ChIP, siRNA knockdown, luciferase reporter, Western blot, xenograft models International journal of biological sciences Medium 35813477
2023 NUF2 inhibits p62-mediated autophagic degradation of transferrin receptor 1 (TFR1) and activates p38/MAPK signaling to promote cholangiocarcinoma progression; NUF2 knockdown reduces TFR1 protein levels by allowing its autophagic degradation. siRNA knockdown, co-immunoprecipitation, Western blot, autophagy flux assays, xenograft models International journal of biological sciences Medium 37056930
2023 NUF2 interacts with HNRNPA2B1 (confirmed by IP/MS and co-IP) and activates the PI3K/AKT/mTOR signaling pathway in ovarian cancer cells; silencing HNRNPA2B1 reduces NUF2 levels, and NUF2 knockdown suppresses PI3K/AKT/mTOR activity. Immunoprecipitation/mass spectrometry, co-immunoprecipitation, siRNA knockdown, Western blot, xenograft models Journal of ovarian research Medium 36670423
2024 NUF2 directly interacts with ERBB3 and inhibits its ubiquitin-mediated proteasomal degradation, thereby stabilizing ERBB3 and activating the PI3K/AKT signaling pathway to promote hepatocellular carcinoma progression and cell cycle regulation. Co-immunoprecipitation, ubiquitination assay, Western blot, siRNA knockdown, xenograft models Translational oncology Medium 38507923
2024 NUF2 directly binds BUB1B (confirmed by co-immunoprecipitation) and regulates the mTORC1 pathway (as indicated by 4EBP1 and S6 phosphorylation); BUB1B overexpression rescues the anti-tumor effects of NUF2 knockdown, placing NUF2 upstream of BUB1B-mediated mTORC1 activation in lung adenocarcinoma. Co-immunoprecipitation, siRNA knockdown, Western blot, in vivo xenograft rescue experiments Journal of thoracic disease Medium 41229848
2024 NUF2 maintains intracellular magnesium homeostasis by epigenetically regulating magnesium transporter expression through H3K4me3 enrichment at their promoters; NUF2 knockdown reduces H3K4me3 at magnesium transporter promoters, lowers intracellular Mg2+, and suppresses PI3K/Akt/mTOR-mediated protein synthesis in anaplastic thyroid cancer cells. ChIP for H3K4me3, intracellular Mg2+ measurement, siRNA knockdown, Western blot, xenograft models Cell death & disease Medium 39242581
2024 NUF2 plays a role in pre-rRNA transcription (primary rate-limiting step of ribosome biogenesis) in interphase human breast epithelial cells; siRNA depletion of NUF2 or its NDC80 complex partners (NDC80, SPC24, SPC25) reduces RNA Pol I largest subunit (POLR1A) levels and decreases pre-rRNA transcription; NUF2/NDC80 depletion activates the nucleolar stress pathway (elevated TP53, p21) reversible by RPL5 co-depletion. Genome-wide siRNA screen, RT-qPCR, Western blot, RNA Pol I activity assays, nucleolar stress pathway epistasis Molecular biology of the cell High 39705402
2024 NUF2 promotes gastric cancer progression by regulating MAPK signaling and G2/M phase transition while inhibiting apoptosis; quercetin was identified as a selective NUF2 inhibitor by virtual docking and microscale thermophoresis, and suppresses tumor growth in CDX, organoid, and PDX models. Live-cell time-lapse imaging, transcriptomics, virtual docking, microscale thermophoresis, CDX/organoid/PDX models, flow cytometry Chinese medical journal Medium 39193700
2025 Single Ndc80 complexes exhibit processive, bi-directional sliding along microtubule walls; plus-end-directed forces induce a mobile catch-bond (increased frictional resistance/restricted sliding toward tip) while forces away from the plus-end trigger a mobile slip-bond; this asymmetric behavior arises from force-dependent modulation of the Nuf2 calponin-homology (CH) domain's microtubule binding, identifying the Nuf2 CH domain as the friction regulator enabling end-coupling and slip-clutch kinetochore mechanics. Ultrafast force-clamp spectroscopy on single Ndc80 complexes, in vitro reconstitution with purified complexes bioRxiv (preprint)preprint High bio_10.1101_2025.03.13.643154
2025 USP7 stabilizes NUF2 protein by mediating its deubiquitination; USP7 depletion reduces NUF2 levels and phenocopies NUF2 knockdown (reduced proliferation, increased apoptosis and ferroptosis); NUF2 in turn positively regulates SLC7A11 expression, and SLC7A11 overexpression reverses the inhibitory effects of USP7 knockdown on ovarian cancer progression. Co-immunoprecipitation, ubiquitination assay, siRNA knockdown, ferroptosis markers (Fe2+, ROS, MDA), xenograft models Experimental cell research Medium 40582584
2025 YY1 directly binds the NUF2 promoter and transcriptionally activates NUF2 expression; NUF2 promotes prostate cancer progression by recruiting p38 and accelerating its phosphorylation, activating the p38/MAPK pathway; fisetin identified as a small-molecule NUF2 inhibitor that reverses NUF2-driven proliferation. Luciferase reporter assay, ChIP, co-immunoprecipitation, phosphorylation assays, in vitro/in vivo proliferation assays Biochemical pharmacology Medium 40187571
2025 SPC25 interacts directly with NUF2 (confirmed by Co-IP and FISH); berberine reduces SPC25 expression, and NUF2 overexpression reverses SPC25-knockdown-mediated inhibition of NSCLC cell growth, invasion, and glycolysis, placing SPC25 upstream of NUF2 within the NDC80 subcomplex. Co-immunoprecipitation, FISH, siRNA knockdown, rescue overexpression, xenograft model Naunyn-Schmiedeberg's archives of pharmacology Medium 39755832

Source papers

Stage 0 corpus · 62 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2004 Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites. Molecular biology of the cell 213 15548592
2003 Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores. Current biology : CB 139 14654001
2003 Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells. Journal of cell science 131 12829748
2006 Activation of CDCA1-KNTC2, members of centromere protein complex, involved in pulmonary carcinogenesis. Cancer research 116 17079454
2001 A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link between the kinetochore function and the spindle checkpoint. Chromosoma 111 11685532
2011 The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis. Molecular biology of the cell 104 21270439
2012 Structural and inhibition insights into carbonic anhydrase CDCA1 from the marine diatom Thalassiosira weissflogii. Biochimie 81 22381359
2007 Human NUF2 interacts with centromere-associated protein E and is essential for a stable spindle microtubule-kinetochore attachment. The Journal of biological chemistry 78 17535814
2009 siRNA-mediated knockdown against CDCA1 and KNTC2, both frequently overexpressed in colorectal and gastric cancers, suppresses cell proliferation and induces apoptosis. Biochemical and biophysical research communications 76 19878654
1994 Nuf2, a spindle pole body-associated protein required for nuclear division in yeast. The Journal of cell biology 64 8188751
2005 Dissociation of the Nuf2-Ndc80 complex releases centromeres from the spindle-pole body during meiotic prophase in fission yeast. Molecular biology of the cell 58 15728720
2021 LncRNA CDKN2B-AS1 stabilized by IGF2BP3 drives the malignancy of renal clear cell carcinoma through epigenetically activating NUF2 transcription. Cell death & disease 48 33608495
2015 siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo. Bioscience reports 47 25370920
2014 Silencing of NUF2 inhibits tumor growth and induces apoptosis in human hepatocellular carcinomas. Asian Pacific journal of cancer prevention : APJCP 43 25374179
2017 Phase I clinical trial of cell division associated 1 (CDCA1) peptide vaccination for castration resistant prostate cancer. Cancer science 42 28498618
2015 Downregulation of NUF2 inhibits tumor growth and induces apoptosis by regulating lncRNA AF339813. International journal of clinical and experimental pathology 40 26045769
2005 The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis. Molecular and cellular biology 39 15713649
2015 Cadmium-containing carbonic anhydrase CDCA1 in marine diatom Thalassiosira weissflogii. Marine drugs 35 25815892
2015 Heterogeneous nuclear ribonucleoprotein K upregulates the kinetochore complex component NUF2 and promotes the tumorigenicity of colon cancer cells. Biochemical and biophysical research communications 31 25701787
2016 Silencing of NUF2 inhibits proliferation of human osteosarcoma Saos-2 cells. European review for medical and pharmacological sciences 29 27049259
2015 Nuf2 is required for chromosome segregation during mouse oocyte meiotic maturation. Cell cycle (Georgetown, Tex.) 28 26054848
2008 Cancer-associated splicing variants of the CDCA1 and MSMB genes expressed in cancer cell lines and surgically resected gastric cancer tissues. Surgery 26 19081476
2020 Correlation of NUF2 Overexpression with Poorer Patient Survival in Multiple Cancers. Cancer research and treatment 25 33421974
2014 Identification of CDCA1-derived long peptides bearing both CD4+ and CD8+ T-cell epitopes: CDCA1-specific CD4+ T-cell immunity in cancer patients. International journal of cancer 23 24734272
2023 NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell Indicator. International journal of molecular sciences 22 36835637
2014 SiRNA-mediated knockdown against NUF2 suppresses tumor growth and induces cell apoptosis in human glioma cells. Cellular and molecular biology (Noisy-le-Grand, France) 22 25481014
2023 NUF2 Drives Cholangiocarcinoma Progression and Migration via Inhibiting Autophagic Degradation of TFR1. International journal of biological sciences 19 37056930
2022 NUF2 Drives Clear Cell Renal Cell Carcinoma by Activating HMGA2 Transcription through KDM2A-mediated H3K36me2 Demethylation. International journal of biological sciences 19 35813477
2022 NUF2 overexpression contributes to epithelial ovarian cancer progression via ERBB3-mediated PI3K-AKT and MAPK signaling axes. Frontiers in oncology 19 36620547
2023 NUF2 promotes tumorigenesis by interacting with HNRNPA2B1 via PI3K/AKT/mTOR pathway in ovarian cancer. Journal of ovarian research 18 36670423
2020 Identification of NUF2 and FAM83D as potential biomarkers in triple-negative breast cancer. PeerJ 18 33005492
2022 FOXM1-mediated NUF2 expression confers temozolomide resistance to human glioma cells by regulating autophagy via the PI3K/AKT/mTOR signaling pathway. Neuropathology : official journal of the Japanese Society of Neuropathology 15 35701983
2022 A noncoding regulatory RNA Gm31932 induces cell cycle arrest and differentiation in melanoma via the miR-344d-3-5p/Prc1 (and Nuf2) axis. Cell death & disease 14 35393397
2012 Interaction of PTPIP51 with Tubulin, CGI-99 and Nuf2 During Cell Cycle Progression. Biomolecules 14 24970130
2022 ANP32E contributes to gastric cancer progression via NUF2 upregulation. Molecular medicine reports 13 35795988
2024 Targeting NUF2 suppresses gastric cancer progression through G2/M phase arrest and apoptosis induction. Chinese medical journal 12 39193700
2021 Poly-SUMO-2/3 chain modification of Nuf2 facilitates CENP-E kinetochore localization and chromosome congression during mitosis. Cell cycle (Georgetown, Tex.) 12 33910471
2021 A missense variant in NUF2, a component of the kinetochore NDC80 complex, causes impaired chromosome segregation and aneuploidy associated with microcephaly and short stature. Human genetics 9 33721060
2021 Expression and clinical significance of NUF2 in kidney renal clear cell carcinoma. Translational andrology and urology 9 34733658
2024 NUF2 regulated the progression of hepatocellular carcinoma through modulating the PI3K/AKT pathway via stabilizing ERBB3. Translational oncology 8 38507923
2021 Evaluation of NUF2 and GMNN Expression in Prostate Cancer: Potential Biomarkers for Prostate Cancer Screening. Reports of biochemistry & molecular biology 6 34604412
2024 NUF2 Expression in Cancer Tissues and Lymph Nodes Suggests Post-Surgery Recurrence of Non-Small Cell Lung Cancer. Diagnostics (Basel, Switzerland) 5 38472943
2024 NUF2 is associated with cancer stem cell characteristics and a potential drug target for prostate cancer. Frontiers in molecular biosciences 5 39703688
2025 Berberine restrains non-small cell lung cancer cell growth, invasion and glycolysis via inactivating the SPC25/NUF2 pathway. Naunyn-Schmiedeberg's archives of pharmacology 4 39755832
2024 Maintenance of magnesium homeostasis by NUF2 promotes protein synthesis and anaplastic thyroid cancer progression. Cell death & disease 4 39242581
2024 NUF2 Promotes Breast Cancer Metastasis via Activating Wnt/β-Catenin Pathways. Frontiers in bioscience (Landmark edition) 4 39614440
2023 FOXM1 promotes neurofibromatosis type 1-associated malignant peripheral nerve sheath tumor progression in a NUF2-dependent manner. Cancer gene therapy 4 37488294
2022 De novo NUF2 variant in a novel inherited bone marrow failure syndrome including microcephaly and renal hypoplasia. British journal of haematology 4 36111525
2019 [Expression of NUF2 in breast cancer and its clinical significance]. Nan fang yi ke da xue xue bao = Journal of Southern Medical University 4 31140425
2024 A role for the kinetochore protein, NUF2, in ribosome biogenesis. Molecular biology of the cell 3 39705402
2025 NUF2 and NEK2 promote malignant progression of gallbladder cancer by remodeling the extracellular matrix. Carcinogenesis 2 40220284
2025 Uncovering novel functions of NUF2 in glioblastoma and MRI-based expression prediction. Scientific reports 2 40890233
2022 Clinical significance and prospective molecular mechanism of NUF2 in gastric cancer exploration: A comprehensive study based on the GeneChip, GEO, Oncomine, and TCGA databases. Medicine 2 35777029
2013 Complementary interhelical interactions between three buried Glu-Lys pairs within three heptad repeats are essential for Hec1-Nuf2 heterodimerization and mitotic progression. The Journal of biological chemistry 2 24129578
2025 NUF2 activated by YY1 promotes prostate cancer malignancy via p38/MAPK signaling axis and serves as a therapeutic target. Biochemical pharmacology 1 40187571
2025 USP7-mediated NUF2 deubiquitination accelerates ovarian cancer progression through regulating SLC7A11 expression. Experimental cell research 1 40582584
2026 Clinical significance and immune effect of expression of NUF2 in lung adenocarcinoma. Medicine 0 41517679
2026 Anti-cancer effects of carvacrol on NUF2, CASP3 and CLDN6 expression in T47D breast cancer cells. Naunyn-Schmiedeberg's archives of pharmacology 0 41569314
2025 Analysis of a cancer-associated mutation in the budding yeast Nuf2 kinetochore protein. microPublication biology 0 40161439
2025 NUF2 promotes the malignant development of lung adenocarcinoma through the mTORC1 signaling pathway via binding to BUB1B. Journal of thoracic disease 0 41229848
2025 Immunohistochemical expression of POC1A, NUF2, and Ki-67 in invasive ductal carcinoma of the breast. American journal of clinical pathology 0 41237374
2025 A core stemness-associated module reveals PLK1, NUF2, KIF23, CDCA8, TOP2A, CENPF, AURKA, and ASPM as key genes in rectal cancer. European journal of medical research 0 41373021