Affinage

CENPB

Major centromere autoantigen B · UniProt P07199

Length
599 aa
Mass
65.2 kDa
Annotated
2026-04-28
100 papers in source corpus 29 papers cited in narrative 29 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CENP-B is a centromeric heterochromatin protein that homodimerizes via a C-terminal four-helix bundle and binds the 17-bp CENP-B box motif in alpha-satellite DNA through dual N-terminal helix-turn-helix domains, inducing DNA kinking and directing nucleosome positioning on alphoid repeats (PMID:2808515, PMID:11726497, PMID:9797455). It directly contacts CENP-A nucleosomes and CENP-C, stabilizing centromeric CENP-C levels and enhancing chromosome segregation fidelity, and is essential for de novo centromere assembly on exogenous alphoid DNA while simultaneously recruiting heterochromatin factors (Suv39h1, HP1) and the Daxx/H3.3 deposition pathway to balance centromeric chromatin identity (PMID:25942623, PMID:12460987, PMID:18160038, PMID:29273057, PMID:32661090). Through its acidic domain, CENP-B recruits chromatin modifiers including ASH1L, which deposits H3K36 methylation to facilitate CENP-A assembly, thereby acting as a nexus between open and heterochromatic centromeric states (PMID:32661090). Despite these roles, CENP-B is dispensable for viability and normal mitosis in knockout mice under standard conditions (PMID:9933410).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1989 High

    The fundamental DNA target of CENP-B was identified: it binds a specific 17-bp CENP-B box within alpha-satellite repeats, establishing CENP-B as a sequence-specific centromeric DNA-binding protein rather than a general chromatin component.

    Evidence Immunoprecipitation of alphoid DNA from HeLa nuclear extract, streptavidin pulldown, and Southwestern blotting

    PMID:2808515

    Open questions at the time
    • Binding affinity not quantified
    • Domain responsible for DNA binding not mapped
    • In vivo functional consequence of CENP-B box binding unknown
  2. 1990 High

    Ultrastructural localization resolved that CENP-B resides throughout centromeric heterochromatin beneath the kinetochore, not at the kinetochore plate itself, and is present at both active and inactive centromeres — establishing its role as an alphoid DNA/heterochromatin-associated factor independent of kinetochore activity.

    Evidence Immunoelectron microscopy with 1-nm gold probes; immunofluorescence on dicentric chromosomes

    PMID:2335558 PMID:2475307

    Open questions at the time
    • Mechanism by which CENP-B associates with heterochromatin versus kinetochore not defined
    • Whether CENP-B contributes to heterochromatin formation or merely resides there was unknown
  3. 1992 High

    Domain architecture was resolved: the N-terminal ~125–158 residues constitute the DNA-binding and centromere-targeting domain, while the C-terminal ~20 kDa mediates homodimerization, and CENP-B dimers can juxtapose two CENP-B box DNAs with high affinity (~6×10⁸ M⁻¹), suggesting a higher-order chromatin organizing role.

    Evidence Truncated recombinant proteins in E. coli, gel mobility-shift assays, proteolytic mapping, in vivo targeting of fused reporter

    PMID:1469042 PMID:1730770 PMID:1740467

    Open questions at the time
    • Atomic structure of neither domain determined
    • Physiological consequence of DNA bundling/looping unresolved
  4. 1998 High

    The structural basis of CENP-B box recognition and chromatin organization was established: NMR revealed a helix-turn-helix fold in the DNA-binding repeat, and reconstituted CENP-B/alphoid DNA/histone complexes showed CENP-B directs nucleosome positioning and bundles separate DNA fragments.

    Evidence NMR solution structure; in vitro nucleosome assembly with NAP-1 and MNase footprinting

    PMID:9451007 PMID:9797455

    Open questions at the time
    • High-resolution co-crystal with DNA not yet available
    • In vivo nucleosome positioning by CENP-B not demonstrated
  5. 1998 High

    Knockout studies showed CENP-B is dispensable for mouse viability, fertility, and kinetochore-centromere ultrastructure, raising the question of what non-essential function it serves.

    Evidence Gene-targeted CENP-B knockout mice, immunofluorescence, electron microscopy

    PMID:9933410

    Open questions at the time
    • Subtle segregation fidelity phenotypes not tested
    • Conditional or stress-dependent roles not examined
  6. 2001 High

    The 2.5 Å crystal structure of the CENP-B DNA-binding domain bound to the CENP-B box revealed a dual helix-turn-helix mechanism that kinks DNA (~59° overall bend) via arginine-rich helix phosphate bridging, providing the atomic basis for sequence-specific recognition.

    Evidence X-ray crystallography at 2.5 Å resolution

    PMID:11726497

    Open questions at the time
    • Full-length CENP-B structure not resolved
    • Structural basis of CENP-B interaction with nucleosomes unknown
  7. 2002 High

    CENP-B was shown to be required for de novo centromere/kinetochore assembly on transfected alphoid DNA, connecting its DNA-binding activity to functional centromere formation on ectopic substrates.

    Evidence MAC/HAC formation assay with CENP-B box point mutations, ChIP for CENP-A and CENP-B

    PMID:12460987

    Open questions at the time
    • Whether CENP-B recruits CENP-A directly or indirectly unknown
    • Role at endogenous centromeres versus de novo context not delineated
  8. 2003 High

    The dimerization domain crystal structure (1.65 Å) revealed a symmetrical antiparallel four-helix bundle geometry suited for bridging distant CENP-B boxes, and yeast two-hybrid confirmed a direct CENP-B–CENP-C interaction.

    Evidence X-ray crystallography; yeast two-hybrid with truncation mapping

    PMID:14522975 PMID:14612452

    Open questions at the time
    • CENP-B–CENP-C interaction awaited reciprocal co-IP validation
    • Structural basis of CENP-C interaction not determined
  9. 2007 High

    A dual role for CENP-B was revealed: it is required for de novo centromere assembly on extrachromosomal alphoid DNA but paradoxically suppresses centromere formation at integrated alphoid sites by promoting H3K9me3 and DNA methylation, establishing CENP-B as a chromatin-state switch.

    Evidence HAC/MAC formation assay with CENP-B knockout/depletion, ChIP for H3K9me3 and DNA methylation

    PMID:18160038

    Open questions at the time
    • Mechanism by which CENP-B recruits H3K9 methyltransferases not identified
    • How context (extrachromosomal vs. integrated) determines outcome unclear
  10. 2015 High

    Direct protein-protein interactions of CENP-B with both the CENP-A N-terminal tail and CENP-C were demonstrated, and CENP-B was shown to stabilize centromeric CENP-C levels and reduce chromosome mis-segregation rates, providing a functional explanation for CENP-B box conservation despite knockout viability.

    Evidence Pulldown/co-IP of CENP-B with CENP-A tail and CENP-C, chromosome mis-segregation assay, quantitative immunofluorescence; preferential nucleosome binding to CENP-A over H3.1 in vitro

    PMID:25916850 PMID:25942623

    Open questions at the time
    • Structural basis of CENP-B–CENP-A tail interaction not resolved
    • Quantitative contribution to segregation fidelity in vivo needs further systems
  11. 2017 Medium

    CENP-B was identified as a beacon for Daxx-mediated H3.3 deposition at centromeres via SUMO-2-dependent interaction, linking CENP-B to centromeric histone variant incorporation beyond CENP-A.

    Evidence Co-IP, ChIP, CENP-B knockdown, SUMO-2 depletion, immunofluorescence

    PMID:29273057

    Open questions at the time
    • Single-lab observation; independent replication needed
    • Whether H3.3 deposition is required for CENP-B's centromere assembly function unclear
  12. 2020 Medium

    The CENP-B acidic domain was identified as the recruitment platform for opposing chromatin modifiers — ASH1L (H3K36 methylation, favoring CENP-A assembly) and Suv39h1/HP1 (heterochromatin) — establishing the molecular basis for CENP-B's role as a chromatin-state nexus.

    Evidence AP-MS of acidic domain interactors, ChIP for H3K36me and H3K9me, de novo CENP-A assembly assay

    PMID:32661090

    Open questions at the time
    • How the balance between ASH1L and Suv39h1 recruitment is regulated is unknown
    • Whether additional signals determine which pathway dominates remains untested
  13. 2023 High

    Cooperative chromatin remodeling between CENP-A and CENP-B was demonstrated at single-molecule resolution: CENP-A incorporation creates open chromatin that increases CENP-B DNA accessibility, and bound CENP-B further opens the fiber and induces nucleosomal DNA unwrapping, revealing a positive feedback loop.

    Evidence Single-molecule fluorescence, cryo-EM, CENP-A depletion with FRAP in cells

    PMID:38086807

    Open questions at the time
    • How this feedback loop is initiated versus maintained at endogenous centromeres is unclear
    • Structural resolution of CENP-B bound to CENP-A nucleosomes at atomic level not achieved

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include: what determines whether CENP-B promotes CENP-A assembly versus heterochromatin at a given locus; how CENP-B cooperates with ASH1L versus Suv39h1 in a context-dependent manner; what is the full-length atomic structure of CENP-B in complex with a CENP-A nucleosome; and whether CENP-B contributes to centromere maintenance during stress or aging in vivo.
  • No full-length CENP-B structure available
  • Context-dependent chromatin switch mechanism unresolved
  • In vivo phenotypes under stress conditions not systematically tested

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003677 DNA binding 8 GO:0005198 structural molecule activity 2 GO:0042393 histone binding 2
Localization
GO:0005694 chromosome 3 GO:0000228 nuclear chromosome 2
Pathway
R-HSA-4839726 Chromatin organization 4 R-HSA-1640170 Cell Cycle 2
Partners
ASH1LCENPACENPCDAXXPARP1PARP2SUV39H1ZFAT
Complex memberships
CENP-B homodimer

Evidence

Reading pass · 29 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1989 CENP-B specifically binds a 17-bp sequence ('CENP-B box') within alphoid alpha-satellite DNA at human centromeres, as demonstrated by immunoprecipitation of cloned alphoid DNA from HeLa nuclear extract with anticentromere sera, and by recovery of CENP-B (80 kD) via biotinylated alphoid DNA/streptavidin pulldown and Southwestern blotting. DNA immunoprecipitation, streptavidin-agarose pulldown, Southwestern blotting, DNA-protein binding assay The Journal of cell biology High 2808515
1992 Purified CENP-B directly binds the CENP-B box with a binding constant of ~6×10^8 M^-1, and forms a complex (complex A) containing two DNA molecules per CENP-B dimer, suggesting CENP-B organizes higher-order chromatin by juxtaposing two CENP-B boxes. Affinity purification, DNA mobility-shift assay, Southwestern blotting, DNase I protection analysis The Journal of cell biology High 1730770
1992 The DNA-binding domain of CENP-B is located within the N-terminal 125 amino acids (containing four predicted alpha-helices), and this domain is separable from the C-terminal dimerization activity, which resides in the last ~20 kD of the molecule. Truncated CENP-B expression in E. coli, gel mobility-shift assay, proteolytic cleavage mapping The Journal of cell biology High 1469042
1992 The N-terminal ~158 residues of CENP-B constitute a centromere localization signal sufficient to target a fused bacterial enzyme to centromeres in vivo, and this domain specifically binds a subset of alpha-satellite DNA monomers in vitro. Expression of epitope-tagged deletion derivatives in HeLa cells, in vitro DNA binding The Journal of cell biology High 1740467
1990 By immunoelectron microscopy, >95% of CENP-B is distributed throughout centromeric heterochromatin beneath the kinetochore outer plate, establishing that CENP-B is not a kinetochore plate component but is specifically associated with alpha-satellite heterochromatin. Immunoelectron microscopy with 1-nm colloidal gold probes The Journal of cell biology High 2335558
1989 CENP-B is present at both active and inactive centromeres of stable dicentric chromosomes (unlike CENP-C, which is absent from inactive centromeres), indicating CENP-B's association is independent of centromere activity and tied to the DNA/heterochromatin rather than kinetochore function per se. Immunofluorescence with monospecific anti-CENP-B and anti-CENP-C antibodies on dicentric chromosome spreads Chromosoma High 2475307
1995 CENP-B forms a homodimer through a C-terminal hydrophobic domain (~59 aa), with each subunit independently binding a CENP-B box via its N-terminal domain; metaphase-specific phosphorylation of CENP-B occurs but does not affect its complex-forming activity. Chemical cross-linking, gel mobility-shift assay, yeast two-hybrid, truncated CENP-B expression, phosphorylation analysis Molecular and cellular biology High 7862152
2001 Crystal structure of the CENP-B DNA-binding domain (129 residues) complexed with CENP-B box DNA at 2.5 Å resolution reveals two helix-turn-helix domains binding adjacent major grooves, inducing a 'kink-straight-kink' DNA bend (~59° overall) via a novel 'phosphate-bridging by arginine-rich helix' mechanism. X-ray crystallography at 2.5 Å resolution The EMBO journal High 11726497
1998 NMR solution structure of the CENP-B DNA-binding domain repeat 1 (RP1) reveals a four-helix bundle with a helix-turn-helix motif; chemical shift perturbation shows RP1 contacts the CENP-B box primarily through the N-terminal basic region and helices 2 and 3. Multidimensional 1H/13C/15N NMR, chemical shift perturbation The EMBO journal High 9451007
2003 Crystal structure of the CENP-B dimerization domain (residues 540–599) at 1.65 Å resolution reveals a symmetrical antiparallel four-helix bundle with a large hydrophobic interface (23 residues per monomer), with N-terminal ends oriented on opposite sides—a geometry suited for capturing two distant CENP-B boxes. X-ray crystallography at 1.65 Å resolution The Journal of biological chemistry High 14522975
1998 CENP-B bound to CENP-B boxes causes nucleosome positioning on alpha-satellite DNA, as shown by micrococcal nuclease footprinting of in vitro-assembled CENP-B/alphoid DNA/core histone complexes; CENP-B dimers also bundle two separate DNA fragments and form intramolecular loops when two CENP-B boxes are on the same DNA. In vitro nucleosome assembly with NAP-1, MNase footprinting, DNA bundling assay Genes to cells High 9797455
2002 Both the CENP-B box DNA sequence and the CENP-B binding activity are required for de novo centromere chromatin assembly (CENP-A, CENP-C, CENP-E loading) in the mammalian artificial chromosome (MAC) formation assay; alphoid DNA with mutated CENP-B boxes fails to support MAC formation or CENP-A assembly. MAC formation assay in HT1080 cells, chromatin immunoprecipitation (ChIP) for CENP-A and CENP-B on synthetic alphoid DNA The Journal of cell biology High 12460987
2007 CENP-B is required for de novo centromere assembly on HAC/MAC (alphoid DNA lacking existing centromere context), but suppresses centromere formation when alphoid DNA integrates into a chromosomal site by enhancing histone H3-K9 trimethylation and DNA methylation, promoting heterochromatin. HAC/MAC formation assay, chromatin immunoprecipitation for H3K9me3 and DNA methylation, genetic (CENP-B knockout/depletion) Cell High 18160038
2015 CENP-B directly binds both the amino-terminal tail of CENP-A and CENP-C; DNA sequence-dependent binding of CENP-B within alpha-satellite repeats stabilizes optimal centromeric levels of CENP-C, and chromosomes lacking CENP-B-bound centromeres (including the Y chromosome) mis-segregate at several-fold higher rates. Pulldown/co-immunoprecipitation of CENP-B with CENP-A tail and CENP-C, chromosome mis-segregation assay, quantitative immunofluorescence Developmental cell High 25942623
2003 CENP-B interacts with CENP-C via yeast two-hybrid and the interaction requires CENP-C domains overlapping with three Mif2 homologous regions; overexpression of CENP-B truncations lacking the CENP-C interaction domain causes abnormal CENP-C duplication at G2 and cell cycle delay at metaphase. Yeast two-hybrid screen, domain mapping with truncated polypeptides in cultured cells The Journal of biological chemistry Medium 14612452
2002 CENP-B interacts with PARP-1 and is poly(ADP-ribosyl)ated following DNA damage induction, as shown by immunoprecipitation and Western blot analyses. Co-immunoprecipitation, Western blot, poly(ADP-ribosyl)ation assay after gamma-irradiation The Journal of biological chemistry Medium 12011073
2002 PARP-2 also co-immunoprecipitates with CENP-B, and both PARP-1 and PARP-2 interactions with CENP-B are lost for CENP-C, indicating a selective interaction at the centromere. Co-immunoprecipitation Human molecular genetics Medium 12217960
2015 CENP-B forms a stable complex preferentially with CENP-A nucleosomes (over H3.1 nucleosomes) in vitro when the CENP-B box is proximal to the nucleosome edge; the DNA-binding domain of CENP-B specifically interacts with the CENP-A–H4 complex but not H3.1–H4; CENP-B binding near the CENP-A nucleosome stabilizes CENP-A on alphoid DNA in human cells. In vitro nucleosome binding assay, pulldown with CENP-A/H4 vs. H3.1/H4 complexes, in vivo CENP-A stability assay Nucleic acids research High 25916850
2013 CENP-B undergoes alpha-N-methylation in human cells, catalyzed primarily by N-terminal RCC1 methyltransferase (NRMT); chromatin-bound CENP-B is primarily trimethylated, and alpha-N-trimethylation enhances CENP-B binding to the CENP-B box in cells. Mass spectrometry identification of modification, NRMT knockdown, ChIP for chromatin-bound CENP-B methylation state, in vivo DNA-binding assay Journal of proteome research Medium 23978223
2005 CpG methylation of the CENP-B box DNA reduces CENP-B binding nearly to non-specific levels, as shown by competition analyses using purified CENP-B DNA-binding domain with methylated vs. unmethylated CENP-B box substrates. Electrophoretic mobility shift assay, competition binding analysis with methylated/unmethylated oligonucleotides The FEBS journal High 15634350
2013 Nap1 (nucleosome assembly protein-1) inhibits non-specific CENP-B binding to nucleosomes and stimulates specific CENP-B binding to CENP-B box sequences within nucleosomes in vitro; in human cells, tethered Nap1 near CENP-B boxes significantly reduces CENP-B binding to HAC or ectopic CENP-B box loci. In vitro nucleosome binding/competition assay, tethering assay in human cells with Nap1, comparison with sNASP control Nucleic acids research High 23325853
2017 CENP-B acts as a beacon for H3.3 deposition at centromeres: CENP-B depletion reduces Daxx association and H3.3 incorporation at centromeres; Daxx–CENP-B interaction is SUMO-2-dependent and requires SUMO-interacting motifs (SIMs) of Daxx; loss of this pathway deregulates H3K9me3, ATRX, and HP1α at centromeres and elevates chromosome instability. Co-immunoprecipitation, ChIP, SUMO-2 depletion, CENP-B knockdown, immunofluorescence Epigenetics & chromatin Medium 29273057
2020 The CENP-B acidic domain recruits histone chaperones and chromatin modifiers including H3K36 methylase ASH1L (promoting open chromatin for CENP-A assembly) as well as Suv39h1 and HP1 (promoting heterochromatin); ASH1L facilitates CENP-A assembly on alphoid DNA, and CENP-B thus acts as a nexus balancing mutually exclusive chromatin states. Affinity purification/mass spectrometry of CENP-B acidic domain interactors, ChIP for H3K36me and H3K9me marks, de novo CENP-A assembly assay on transfected alphoid DNA Journal of cell science Medium 32661090
2007 Herpes simplex virus ICP0, an E3 ubiquitin ligase, induces proteasomal degradation of CENP-B in infected or ICP0-expressing human and mouse cells, demonstrating CENP-B can be ubiquitinated and degraded via ICP0's ligase activity. ICP0 expression in human/mouse cells, proteasome inhibitor rescue, Western blot for CENP-B levels FEBS letters Medium 17258208
1998 CENP-B is a non-essential gene in mice: cenpB-null mice are viable and healthy with intact centromere-kinetochore complexes and normal mitosis, as shown by immunofluorescence and electron microscopy of mouse embryo fibroblasts. Gene targeting/knockout in mouse, immunofluorescence, electron microscopy Chromosoma High 9933410
2015 ADA3 associates with CENP-B through ADA3's N-terminus (shown by deletional analysis and proximity ligation assay); ADA3 knockdown decreases CENP-B loading onto centromeres, and a CENP-B-binding-deficient ADA3 mutant fails to rescue cell proliferation. Proximity ligation assay, immunofluorescence, deletional mapping, ADA3 knockdown with ChIP-like centromere loading assay The Journal of biological chemistry Medium 26429915
2021 CENP-B is required for centromeric localization of the zinc-finger transcriptional regulator ZFAT: CENP-B ectopic expression drives ZFAT accumulation at centromeres; ZFAT co-immunoprecipitates with the acidic domain of CENP-B; CENP-B knockdown reduces centromeric ZFAT levels and decreases centromeric noncoding RNA transcription. Co-immunoprecipitation, ectopic CENP-B expression, CENP-B knockdown, immunofluorescence, noncoding RNA quantification The Journal of biological chemistry Medium 34547289
2008 FRET measurements in living human HEp-2 cells demonstrate that CENP-A and CENP-B are within <10 nm of each other at centromere locations, indicating direct molecular proximity; CENP-T also associates with both CENP-A and CENP-B by acceptor-bleaching FRET in live cells. Fluorescence intensity- and lifetime-based FRET in living cells Chembiochem Medium 18072184
2023 Single-molecule fluorescence and cryo-EM show that CENP-A incorporation into chromatin creates a dynamic, open chromatin state that increases CENP-B DNA accessibility; bound CENP-B further opens the chromatin fiber and induces nucleosomal DNA unwrapping; removal of CENP-A increases CENP-B mobility in cells, showing cooperative centromeric chromatin remodeling. Single-molecule fluorescence microscopy, cryo-electron microscopy, CENP-A depletion in cells with FRAP Nature communications High 38086807

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1989 A human centromere antigen (CENP-B) interacts with a short specific sequence in alphoid DNA, a human centromeric satellite. The Journal of cell biology 613 2808515
1987 Molecular cloning of cDNA for CENP-B, the major human centromere autoantigen. The Journal of cell biology 417 2435739
1989 Visualization of centromere proteins CENP-B and CENP-C on a stable dicentric chromosome in cytological spreads. Chromosoma 282 2475307
1993 A functional marker centromere with no detectable alpha-satellite, satellite III, or CENP-B protein: activation of a latent centromere? American journal of human genetics 246 7684888
2002 CENP-B box is required for de novo centromere chromatin assembly on human alphoid DNA. The Journal of cell biology 244 12460987
1990 CENP-B: a major human centromere protein located beneath the kinetochore. The Journal of cell biology 215 2335558
2015 DNA Sequence-Specific Binding of CENP-B Enhances the Fidelity of Human Centromere Function. Developmental cell 213 25942623
1992 Centromere protein B assembles human centromeric alpha-satellite DNA at the 17-bp sequence, CENP-B box. The Journal of cell biology 205 1730770
2007 CENP-B controls centromere formation depending on the chromatin context. Cell 199 18160038
1997 Centromeres, CENP-B and Tigger too. Trends in genetics : TIG 163 9097724
1994 Distribution of CENP-B boxes reflected in CREST centromere antigenic sites on long-range alpha-satellite DNA arrays of human chromosome 21. Human molecular genetics 140 7987298
1992 A human centromere protein, CENP-B, has a DNA binding domain containing four potential alpha helices at the NH2 terminus, which is separable from dimerizing activity. The Journal of cell biology 118 1469042
1998 The cenpB gene is not essential in mice. Chromosoma 114 9933410
1991 CENP-B is a highly conserved mammalian centromere protein with homology to the helix-loop-helix family of proteins. Chromosoma 112 1893793
1987 Analysis of anticentromere autoantibodies using cloned autoantigen CENP-B. Proceedings of the National Academy of Sciences of the United States of America 111 2440036
2002 Centromere proteins Cenpa, Cenpb, and Bub3 interact with poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated. The Journal of biological chemistry 100 12011073
1992 Identification of a subdomain of CENP-B that is necessary and sufficient for localization to the human centromere. The Journal of cell biology 100 1740467
2001 Crystal structure of the CENP-B protein-DNA complex: the DNA-binding domains of CENP-B induce kinks in the CENP-B box DNA. The EMBO journal 92 11726497
2002 Fission yeast CENP-B homologs nucleate centromeric heterochromatin by promoting heterochromatin-specific histone tail modifications. Genes & development 91 12130537
1995 CENP-B binds a novel centromeric sequence in the Asian mouse Mus caroli. Molecular and cellular biology 91 7623797
2002 Poly(ADP-ribose) polymerase 2 localizes to mammalian active centromeres and interacts with PARP-1, Cenpa, Cenpb and Bub3, but not Cenpc. Human molecular genetics 79 12217960
2010 CENP-B preserves genome integrity at replication forks paused by retrotransposon LTR. Nature 76 21151105
2004 The role of CENP-B and alpha-satellite DNA: de novo assembly and epigenetic maintenance of human centromeres. Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 73 15289662
1995 Presence and abundance of CENP-B box sequences in great ape subsets of primate-specific alpha-satellite DNA. Journal of molecular evolution 70 7563136
1996 Surprising deficiency of CENP-B binding sites in African green monkey alpha-satellite DNA: implications for CENP-B function at centromeres. Molecular and cellular biology 64 8756673
1995 Analysis of protein-DNA and protein-protein interactions of centromere protein B (CENP-B) and properties of the DNA-CENP-B complex in the cell cycle. Molecular and cellular biology 61 7862152
1998 In vitro assembly of the CENP-B/alpha-satellite DNA/core histone complex: CENP-B causes nucleosome positioning. Genes to cells : devoted to molecular & cellular mechanisms 60 9797455
1987 Detection of anticentromere antibodies using cloned autoantigen CENP-B. Arthritis and rheumatism 60 3435569
2003 CENP-B interacts with CENP-C domains containing Mif2 regions responsible for centromere localization. The Journal of biological chemistry 57 14612452
1990 Partial deletion of alpha satellite DNA associated with reduced amounts of the centromere protein CENP-B in a mitotically stable human chromosome rearrangement. Molecular and cellular biology 55 2247061
2015 Stable complex formation of CENP-B with the CENP-A nucleosome. Nucleic acids research 52 25916850
2013 Identification of novel α-n-methylation of CENP-B that regulates its binding to the centromeric DNA. Journal of proteome research 50 23978223
1997 A centromere DNA-binding protein from fission yeast affects chromosome segregation and has homology to human CENP-B. The Journal of cell biology 48 9024682
2007 Centromeric protein CENP-B proteasomal degradation induced by the viral protein ICP0. FEBS letters 47 17258208
2020 From evolution to function: Two sides of the same CENP-B coin? Experimental cell research 43 32173469
2005 CpG methylation of the CENP-B box reduces human CENP-B binding. The FEBS journal 43 15634350
2000 Fission yeast homologs of human CENP-B have redundant functions affecting cell growth and chromosome segregation. Molecular and cellular biology 42 10733588
1999 Single base discrimination of CENP-B repeats on mouse and human Chromosomes with PNA-FISH. Mammalian genome : official journal of the International Mammalian Genome Society 41 9892726
2020 CENP-B creates alternative epigenetic chromatin states permissive for CENP-A or heterochromatin assembly. Journal of cell science 40 32661090
2012 CENP-B cooperates with Set1 in bidirectional transcriptional silencing and genome organization of retrotransposons. Molecular and cellular biology 40 22907751
1997 Purification and characterization of a CENP-B homologue protein that binds to the centromeric K-type repeat DNA of Schizosaccharomyces pombe. Proceedings of the National Academy of Sciences of the United States of America 40 9237993
2006 Anti-endothelial cell antibodies from patients with limited cutaneous systemic sclerosis bind to centromeric protein B (CENP-B). Clinical immunology (Orlando, Fla.) 39 16580263
2006 CENP-B box and pJalpha sequence distribution in human alpha satellite higher-order repeats (HOR). Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 38 17115329
1995 High prevalence of antibodies to recombinant CENP-B in primary biliary cirrhosis: nuclear immunofluorescence patterns and ELISA reactivities. Journal of gastroenterology and hepatology 37 8527711
1994 Anticentromere autoantibodies. Evaluation of an ELISA using recombinant fusion protein CENP-B as antigen. Arthritis and rheumatism 36 8129780
1992 Molecular cloning of a major CENP-B epitope and its use for the detection of anticentromere autoantibodies. Molecular biology reports 36 1545784
2017 CENP-B protects centromere chromatin integrity by facilitating histone deposition via the H3.3-specific chaperone Daxx. Epigenetics & chromatin 34 29273057
1998 A helix-turn-helix structure unit in human centromere protein B (CENP-B). The EMBO journal 34 9451007
2011 Anti-centromere antibodies in a large cohort of systemic sclerosis patients: comparison between immunofluorescence, CENP-A and CENP-B ELISA. Clinica chimica acta; international journal of clinical chemistry 33 21756890
1992 Anti-helix-loop-helix domain antibodies: discovery of autoantibodies that inhibit DNA binding activity of human centromere protein B (CENP-B). Journal of biochemistry 32 1377670
2008 Audiovestibular manifestations in patients with limited systemic sclerosis and centromere protein-B (CENP-B) antibodies. Medicine 30 18520322
1995 Classification of multi-helical DNA-binding domains and application to predict the DBD structures of sigma factor, LysR, OmpR/PhoB, CENP-B, Rapl, and Xy1S/Ada/AraC. FEBS letters 30 7556672
2008 Assembly of the inner kinetochore proteins CENP-A and CENP-B in living human cells. Chembiochem : a European journal of chemical biology 29 18072184
2000 A satellite DNA containing CENP-B box-like motifs is present in the antarctic scallop Adamussium colbecki. Gene 29 10773457
1998 Antibodies to Ro/La, Cenp-B, and snRNPs antigens in autoimmune hepatitis of North America versus Asia: patterns of immunofluorescence, ELISA reactivities, and HLA association. Digestive diseases and sciences 29 9635626
2008 Live-cell imaging reveals sustained centromere binding of CENP-T via CENP-A and CENP-B. Journal of biophotonics 26 19412974
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