Affinage

CBX8

Chromobox protein homolog 8 · UniProt Q9HC52

Length
389 aa
Mass
43.4 kDa
Annotated
2026-04-28
100 papers in source corpus 18 papers cited in narrative 18 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CBX8 is a chromodomain-containing Polycomb group protein that functions as a context-dependent transcriptional regulator, mediating both gene repression within PRC1 complexes and non-canonical transcriptional activation at distinct genomic loci. Its chromodomain drives chromatin association through dual recognition of H3K27me3 and DNA, and CBX8 occupies bivalent promoters where it represses the INK4A-ARF locus to bypass senescence, silences differentiation regulators in germinal center B cells via a non-canonical PRC1-BCOR complex assembled by EZH2/BCL6, and facilitates activation of differentiation genes during ES cell differentiation through Cbx7-to-Cbx8 exchange (PMID:17332741, PMID:27505670, PMID:25500566, PMID:30597065). CBX8 is required for MLL-AF9-driven leukemogenesis through direct interaction with MLL-AF9 and TIP60, enabling transcriptional activation of HOX genes, and its chromodomain is pharmacologically targetable to block this oncogenic program (PMID:22094252, PMID:31755685). CBX8 also participates in DNA damage repair by rapid PARP1- and TRIM33-dependent recruitment to damage sites, where it promotes both homologous recombination and non-homologous end joining (PMID:27555324).

Mechanistic history

Synthesis pass · year-by-year structured walk · 9 steps
  1. 2007 High

    Establishing CBX8 as a PRC1 component that directly represses the INK4A-ARF tumor suppressor locus resolved how Polycomb chromobox proteins control replicative senescence and cellular immortalization.

    Evidence ChIP at INK4A-ARF, ectopic expression in human/mouse fibroblasts causing senescence bypass

    PMID:17332741

    Open questions at the time
    • How CBX8 is selectively recruited to INK4A-ARF versus other PRC1 targets was not addressed
    • Whether CBX8's chromodomain histone-binding activity is required for INK4A-ARF repression was untested
  2. 2011 High

    Demonstrating that CBX8 physically interacts with MLL-AF9 and TIP60 and is essential for HOX gene activation and leukemic transformation revealed an unexpected non-canonical activating role for a Polycomb protein in oncogenesis.

    Evidence Co-IP, point-mutation disruption of CBX8–MLL-AF9 interaction, Cbx8 knockout mice, retroviral transformation assays

    PMID:22094252

    Open questions at the time
    • Whether TIP60 acetyltransferase activity is the effector downstream of CBX8 recruitment was not tested
    • Structural basis of the CBX8–MLL-AF9 interaction was not determined
  3. 2014 High

    Showing that Cbx8 transiently replaces Cbx7 at bivalent promoters during ES cell differentiation to enable gene activation established a Polycomb subunit-switching mechanism controlling developmental transcription.

    Evidence Endogenous Cbx8 ChIP-seq, Co-IP, siRNA depletion, microarray in differentiating ES cells

    PMID:25500566

    Open questions at the time
    • The signal triggering the Cbx7-to-Cbx8 switch was not identified
    • Whether the activating PRC1 complex containing Cbx8 has a distinct subunit composition from canonical PRC1 was not fully resolved
  4. 2016 High

    Two studies expanded CBX8's mechanistic repertoire: identification of a non-canonical PRC1-BCOR-CBX8 complex at bivalent promoters in germinal center B cells, and discovery of rapid PARP1/TRIM33-dependent CBX8 recruitment to DNA damage sites required for both HR and NHEJ.

    Evidence ChIP-seq and Co-IP with GC formation assays (GC study); live-cell imaging, Co-IP with TRIM33, HR/NHEJ reporter assays (DNA damage study)

    PMID:27505670 PMID:27555324

    Open questions at the time
    • How TRIM33 physically recruits CBX8 to damage sites and whether the chromodomain is required for this function were not determined
    • Whether PRC1-BCOR-CBX8 complex has distinct enzymatic activity (e.g., ubiquitin ligase) compared to canonical PRC1 was not tested
  5. 2017 High

    Context-dependent transcriptional regulation by CBX8 was elaborated in cancer: CBX8 activates EGR1/AKT/β-catenin signaling in hepatocellular carcinoma while repressing Snail to suppress EMT in esophageal squamous cell carcinoma, illustrating dual activator/repressor functions at different loci.

    Evidence ChIP, Co-IP, luciferase reporters, siRNA/overexpression, in vivo metastasis models in HCC and ESCC

    PMID:28912889 PMID:29066512

    Open questions at the time
    • What determines whether CBX8 acts as an activator versus repressor at a given promoter is unknown
    • Whether these cancer-context functions depend on PRC1 complex membership was not tested
  6. 2018 Medium

    Structure-activity analysis of the CBX8 chromodomain using DNA-encoded library selections identified selective peptidomimetic ligands, defining the structural requirements distinguishing CBX8 from CBX7 chromodomain binding.

    Evidence DNA-encoded library affinity selection with fluorescence polarization validation

    PMID:29309209

    Open questions at the time
    • No cell-based validation of these ligands was reported in this study
    • Co-crystal structure of ligand–CBX8 ChD was not obtained
  7. 2019 High

    Structural and biochemical characterization demonstrated that the CBX8 chromodomain binds both H3K27me3 and DNA simultaneously, and a cell-permeable chromodomain inhibitor validated this dual-binding mode as essential for CBX8 chromatin association and MLL-AF9 target gene expression.

    Evidence In vitro binding assays, structural characterization, mutagenesis, ChIP; DNA-encoded library-derived inhibitor SW2_110A with cellular chromatin association and proliferation assays in THP1 cells

    PMID:30597065 PMID:31755685

    Open questions at the time
    • Full atomic-resolution structure of CBX8 ChD bound simultaneously to nucleosomal DNA and H3K27me3 is lacking
    • Whether inhibitor efficacy extends to in vivo leukemia models was not shown
  8. 2019 Medium

    Non-canonical transcriptional activation by CBX8 was further demonstrated at the LGR5 locus, where CBX8 recruits KMT2b to maintain H3K4me3, and CBX8 mRNA itself is stabilized by m6A modification, linking epitranscriptomic regulation to Polycomb function.

    Evidence ChIP-seq, RNA-seq, Co-IP, dual luciferase reporter assay

    PMID:31849331

    Open questions at the time
    • Direct interaction between CBX8 and KMT2b was not structurally characterized
    • Which m6A writer/reader mediates CBX8 mRNA stabilization was not fully identified
  9. 2021 Medium

    Identification of KPNA2 as a CBX8 nuclear import factor and demonstration that nuclear CBX8 recruits BCOR to repress PRDM1 in bladder cancer connected nuclear trafficking to CBX8's repressive output.

    Evidence Co-IP, ChIP, siRNA/overexpression, in vivo mouse model

    PMID:33751128

    Open questions at the time
    • Whether KPNA2-dependent import is regulated or constitutive was not addressed
    • Whether CBX8 uses the same BCOR recruitment mechanism in GC B cells and bladder cancer was not compared

Open questions

Synthesis pass · forward-looking unresolved questions
  • The central unresolved question is what determines whether CBX8 acts as a transcriptional repressor (via PRC1/BCOR) or activator (via MLL-AF9/TIP60/KMT2b) at a given locus, and how its dual H3K27me3-DNA binding mode integrates with these distinct complexes genome-wide.
  • No systematic genome-wide comparison of CBX8 activating versus repressive targets with matched complex composition
  • In vivo efficacy of CBX8 chromodomain inhibitors has not been demonstrated
  • Whether the DNA damage response role of CBX8 is separable from its PRC1-associated functions is unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 6 GO:0042393 histone binding 4 GO:0003677 DNA binding 1
Localization
GO:0005634 nucleus 4 GO:0005694 chromosome 4
Pathway
R-HSA-4839726 Chromatin organization 5 R-HSA-74160 Gene expression (Transcription) 4 R-HSA-1643685 Disease 3 R-HSA-73894 DNA Repair 1
Partners
BCOREGR1KMT2BMLL-AF9RING1BTIP60TRIM33YBX1
Complex memberships
PRC1PRC1-BCOR-CBX8

Evidence

Reading pass · 18 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2007 CBX8, as part of a PRC1 complex, directly binds to the INK4A-ARF locus and represses it, leading to bypass of senescence and cellular immortalization when CBX8 is ectopically expressed in diploid human and mouse fibroblasts. ChIP (location analysis), ectopic expression, gene expression profiling, loss-of-function in fibroblasts The EMBO journal High 17332741
2011 CBX8 interacts with MLL-AF9 and TIP60, and is required for MLL-AF9-induced transcriptional activation of HOX genes and leukemic transformation; point mutations disrupting the CBX8–MLL-AF9 interaction abrogate HOX gene upregulation and abolish leukemogenesis. Co-immunoprecipitation, point mutation analysis, Cbx8 knockout mice, retroviral transformation assays, gene expression analysis Cancer cell High 22094252
2016 CBX8 is induced in germinal center B cells, binds H3K27me3 at bivalent promoters via its chromodomain, and is required for stable association of a non-canonical PRC1-BCOR-CBX8 complex assembled by EZH2 and BCL6, mediating histone modifications and GC formation. ChIP-seq, Co-IP, genetic knockdown, functional GC formation assays Cancer cell High 27505670
2014 A Cbx8-containing PRC1 complex facilitates transcriptional activation of differentiation genes during ES cell differentiation; Cbx8 transiently recruits to bivalent promoters during early gene activation and is required for efficient activation, with exchange of Cbx7 for Cbx8 being necessary for this process. ChIP-seq of endogenous Cbx8, interaction analysis (co-IP), siRNA depletion, microarray gene expression PLoS genetics High 25500566
2019 The CBX8 chromodomain drives chromatin association through dual binding to both H3K27me3 histone marks and DNA; structural characterization revealed how histone and DNA binding integrate, and both activities were required for CBX8 chromatin association in vivo. Biochemical binding assays (in vitro), structural characterization, ChIP in cells, mutagenesis Nucleic acids research High 30597065
2019 A selective, cell-permeable CBX8 chromodomain inhibitor (SW2_110A) developed via DNA-encoded library selection binds the CBX8 ChD with Kd ~800 nM, specifically inhibits CBX8 chromatin association in cells, and reduces MLL-AF9 target gene (HOXA9) expression and proliferation of THP1 leukemia cells, validating the chromodomain as necessary for CBX8's role in MLL-AF9 transcriptional activation. DNA-encoded library selection, fluorescence polarization, cell-based chromatin association assay, gene expression analysis, proliferation assay ACS chemical biology High 31755685
2017 CBX8 activates AKT/β-catenin signaling in hepatocellular carcinoma in a non-canonical manner: CBX8 directly binds the EGR1 promoter to enhance transcription and also interacts with EGR1 protein in the nucleus to prevent its degradation; additionally, CBX8 upregulates miR-365a-3p which promotes nuclear β-catenin localization by targeting ZNRF1. ChIP, co-immunoprecipitation, luciferase reporter assay, siRNA knockdown, ectopic expression, in vivo xenograft Cancer research High 29066512
2016 CBX8 accumulates rapidly at sites of DNA damage within 30 seconds in a PARP1-dependent (but ATM-independent) manner; CBX8 biochemically interacts with TRIM33, and its recruitment to DNA damage sites requires TRIM33; CBX8 knockdown reduces efficiency of both homologous and non-homologous recombination and increases sensitivity to ionizing radiation. Live-cell imaging, co-immunoprecipitation, siRNA knockdown, DNA repair assays (HR/NHEJ reporters), clonogenic survival The Journal of biological chemistry High 27555324
2014 CBX8 knockdown in colorectal cancer cells inhibits proliferation (partly by increasing p53 and downstream effectors) but paradoxically enhances migration, invasion and metastasis through direct upregulation of integrin β4 (ITGB4), which in turn decreases RhoA activity. siRNA knockdown, in vitro migration/invasion assays, in vivo metastasis assay, gene expression analysis, ChIP Oncotarget Medium 25360999
2017 CBX8 suppresses tumor metastasis in esophageal squamous cell carcinoma by directly binding the Snail promoter and repressing its transcription, thereby inhibiting EMT; CBX8 expression inversely correlates with Snail in ESCC tissues. ChIP, luciferase reporter assay, siRNA/overexpression, migration/invasion assays, in vivo metastasis model Theranostics Medium 28912889
2019 CBX8 promotes LGR5 transcription in a non-canonical manner by recruiting KMT2b to the LGR5 promoter, maintaining H3K4me3 status; additionally, m6A methylation upregulates CBX8 by maintaining CBX8 mRNA stability. ChIP-seq, RNA-seq, ChIP, co-immunoprecipitation, dual luciferase reporter assay, IP Molecular cancer Medium 31849331
2021 KPNA2 promotes nuclear import of CBX8; once nuclear, CBX8 downregulates PRDM1 by recruiting BCOR to the PRDM1 promoter region, leading to suppression of the PRDM1/c-FOS pathway and promotion of bladder cancer progression. Co-immunoprecipitation, ChIP, siRNA knockdown, overexpression, in vivo mouse model Journal of translational medicine Medium 33731128
2019 CBX8 promotes tumorigenesis in esophageal squamous cell carcinoma by downregulating APAF1 expression (identified via transcriptome sequencing), thereby suppressing the mitochondrial apoptotic pathway and conferring radioresistance. Transcriptome sequencing, ChIP (implied), siRNA knockdown, apoptosis assays, irradiation survival assay Gene Medium 31255735
2015 CBX8 suppresses Sirtinol-induced premature senescence in K562 CML cells by enhancing phosphorylation of AKT1, p27KIP1, and RB, thereby promoting E2F1 transcriptional activity; CBX8 knockdown reverses this effect, placing CBX8 in the AKT-RB-E2F1 pathway. CBX8 overexpression and siRNA knockdown, Western blot for pathway components, senescence assays Biochemical and biophysical research communications Medium 26718407
2020 CBX8 promotes invasion and migration in glioblastoma, breast cancer, and lung cancer by binding the WNK2 promoter to suppress WNK2 expression; reduced WNK2 leads to elevated MMP2 and RAC1 activity, which drive invasion and migration respectively. ChIP, siRNA knockdown, overexpression, invasion/migration assays, in vivo xenograft Molecular therapy oncolytics Medium 33251331
2015 IGF1 increases CBX8 expression in HCT116 colon cancer cells; CBX8 overexpression promotes proliferation (upregulates CyclinD1) and knockdown induces apoptosis, placing CBX8 downstream of IGF1 signaling in colon cancer. Gene silencing (siRNA), overexpression, Western blot, RT-PCR, cell proliferation assay Cell biochemistry and biophysics Low 25398592
2019 CBX8 interacts with YBX1, and YBX1-mediated regulation of CyclinD1 is required for CBX8-driven cell cycle progression and proliferation in hepatocellular carcinoma cells; YBX1 knockdown compromises proliferation of CBX8-overexpressing cells. Co-immunoprecipitation, siRNA knockdown, overexpression, Western blot, GSEA Aging Low 31495785
2018 DNA-encoded positional scanning library selections against the CBX8 chromodomain identified peptidomimetic ligands with increased potency and selectivity for CBX8 ChD over CBX7 ChD, characterizing the structural requirements for selective ChD binding. DNA-encoded library affinity selection, fluorescence polarization, sequencing SLAS discovery Medium 29309209

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2011 PC3 is a cell line characteristic of prostatic small cell carcinoma. The Prostate 375 21432867
2008 Effects of miR-34a on cell growth and chemoresistance in prostate cancer PC3 cells. Biochemical and biophysical research communications 279 18834855
1998 A SWI/SNF-related chromatin remodeling complex, E-RC1, is required for tissue-specific transcriptional regulation by EKLF in vitro. Cell 263 9778250
2001 In search of a function for the TIS21/PC3/BTG1/TOB family. FEBS letters 216 11377414
2016 EZH2 and BCL6 Cooperate to Assemble CBX8-BCOR Complex to Repress Bivalent Promoters, Mediate Germinal Center Formation and Lymphomagenesis. Cancer cell 201 27505670
2008 PC3 human prostate carcinoma cell holoclones contain self-renewing tumor-initiating cells. Cancer research 184 18339862
2007 Bypass of senescence by the polycomb group protein CBX8 through direct binding to the INK4A-ARF locus. The EMBO journal 160 17332741
2011 CBX8, a polycomb group protein, is essential for MLL-AF9-induced leukemogenesis. Cancer cell 136 22094252
1993 Purification and characterization of the prohormone convertase PC1(PC3). The Journal of biological chemistry 116 8449925
2019 m6A modification-mediated CBX8 induction regulates stemness and chemosensitivity of colon cancer via upregulation of LGR5. Molecular cancer 108 31849331
2000 Radiation induces upregulation of cyclooxygenase-2 (COX-2) protein in PC-3 cells. International journal of radiation oncology, biology, physics 100 10974444
1995 Differences in pH optima and calcium requirements for maturation of the prohormone convertases PC2 and PC3 indicates different intracellular locations for these events. The Journal of biological chemistry 87 7836407
1998 5-Lipoxygenase inhibitors reduce PC-3 cell proliferation and initiate nonnecrotic cell death. The Prostate 83 9792133
1994 Effect of epidermal growth factor on prostate cancer cell line PC3 growth and invasion. The Prostate 80 8290389
2007 Anti-proliferative effects of evodiamine on human prostate cancer cell lines DU145 and PC3. Journal of cellular biochemistry 78 17340628
2009 Luteolin inhibits invasion of prostate cancer PC3 cells through E-cadherin. Molecular cancer therapeutics 77 19509250
2007 Lysophosphatidic acid induces prostate cancer PC3 cell migration via activation of LPA(1), p42 and p38alpha. Biochimica et biophysica acta 75 17531530
2004 Dual control of neurogenesis by PC3 through cell cycle inhibition and induction of Math1. The Journal of neuroscience : the official journal of the Society for Neuroscience 75 15056715
2017 CBX8 Exhibits Oncogenic Activity via AKT/β-Catenin Activation in Hepatocellular Carcinoma. Cancer research 69 29066512
2017 Inhibition of ANO1 by luteolin and its cytotoxicity in human prostate cancer PC-3 cells. PloS one 64 28362855
2002 BTG2(TIS21/PC3) induces neuronal differentiation and prevents apoptosis of terminally differentiated PC12 cells. Oncogene 64 12360398
1996 Effects of alendronate and taxol on PC-3 ML cell bone metastases in SCID mice. Invasion & metastasis 64 9186547
2007 Inhibition of medulloblastoma tumorigenesis by the antiproliferative and pro-differentiative gene PC3. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 59 17371797
2019 Optimization of Ligands Using Focused DNA-Encoded Libraries To Develop a Selective, Cell-Permeable CBX8 Chromodomain Inhibitor. ACS chemical biology 58 31755685
2014 A Cbx8-containing polycomb complex facilitates the transition to gene activation during ES cell differentiation. PLoS genetics 55 25500566
2001 Effect of scopoletin on PC3 cell proliferation and apoptosis. Acta pharmacologica Sinica 55 11749777
2014 Paradoxical role of CBX8 in proliferation and metastasis of colorectal cancer. Oncotarget 51 25360999
2009 The glutamatergic system expression in human PC-3 and LNCaP prostate cancer cells. Anticancer research 49 19331175
2002 PC3 potentiates NGF-induced differentiation and protects neurons from apoptosis. Neuroreport 45 11930152
2018 Robustness of In Vitro Selection Assays of DNA-Encoded Peptidomimetic Ligands to CBX7 and CBX8. SLAS discovery : advancing life sciences R & D 42 29309209
2017 CBX8 Suppresses Tumor Metastasis via Repressing Snail in Esophageal Squamous Cell Carcinoma. Theranostics 42 28912889
2003 The prohormone processing enzyme PC3 is a lipid raft-associated transmembrane protein. Biochemistry 41 12950171
2019 Characterization of CD44 intracellular domain interaction with RUNX2 in PC3 human prostate cancer cells. Cell communication and signaling : CCS 40 31331331
2020 Beneficial effects of genistein in suppression of proliferation, inhibition of metastasis, and induction of apoptosis in PC3 prostate cancer cells. Archives of physiology and biochemistry 39 31985311
1999 Melatonin receptors in PC3 human prostate tumor cells. Journal of pineal research 39 10340723
2014 Nobiletin suppresses cell viability through AKT pathways in PC-3 and DU-145 prostate cancer cells. BMC pharmacology & toxicology 38 25342300
2012 Cell killing and radiosensitizing effects of atorvastatin in PC3 prostate cancer cells. Journal of radiation research 38 22510595
2000 PC3 overexpression affects the pattern of cell division of rat cortical precursors. Mechanisms of development 37 10585559
2016 MtDNA depleted PC3 cells exhibit Warburg effect and cancer stem cell features. Oncotarget 36 27248169
2015 NES1/KLK10 gene represses proliferation, enhances apoptosis and down-regulates glucose metabolism of PC3 prostate cancer cells. Scientific reports 36 26616394
2014 Hsp90 C-terminal inhibitors exhibit antimigratory activity by disrupting the Hsp90α/Aha1 complex in PC3-MM2 cells. ACS chemical biology 36 25402753
2020 TNF-α controls Lipocalin-2 expression in PC-3 prostate cancer cells. Cytokine 35 32712458
2019 Engagement of DNA and H3K27me3 by the CBX8 chromodomain drives chromatin association. Nucleic acids research 31 30597065
2018 MicroRNA-652 induces NED in LNCaP and EMT in PC3 prostate cancer cells. Oncotarget 29 29721191
2008 Simvastatin inducing PC3 prostate cancer cell necrosis mediated by calcineurin and mitochondrial dysfunction. Journal of bioenergetics and biomembranes 29 18679777
2000 Effects of triptorelin, a gonadotropin-releasing hormone agonist, on the human prostatic cell lines PC3 and LNCaP. Journal of andrology 29 10901441
2022 Quorum Sensing Inhibitory and Quenching Activity of Bacillus cereus RC1 Extracts on Soft Rot-Causing Bacteria Lelliottia amnigena. ACS omega 28 35910130
2015 Galectin-3 Overrides PTRF/Cavin-1 Reduction of PC3 Prostate Cancer Cell Migration. PloS one 28 25942420
2003 Parathyroid hormone-related protein upregulates integrin expression via an intracrine pathway in PC-3 prostate cancer cells. Regulatory peptides 28 12686457
1997 Expression of PC3, carboxypeptidase E and enkephalin in human monocyte-derived macrophages as a tool for genetic studies. FEBS letters 28 9074629
2018 Androgen receptor expression reduces stemness characteristics of prostate cancer cells (PC3) by repression of CD44 and SOX2. Journal of cellular biochemistry 26 30206982
2008 Studies on the origin of the voltammetric response of the PC-3 cell suspension. Talanta 26 19203631
2022 Actinomycin D: a novel Pseudomonas aeruginosa quorum sensing inhibitor from the endophyte Streptomyces cyaneochromogenes RC1. World journal of microbiology & biotechnology 25 35904625
2002 Chemosensitivity of human prostate cancer cells PC3 and LNCaP to genistein isoflavone and beta-lapachone. Biology of the cell 25 12000145
1995 Expression of activin and activin receptors in pc3 human prostatic-cancer cells. International journal of oncology 25 21556577
2021 KPNA2 interaction with CBX8 contributes to the development and progression of bladder cancer by mediating the PRDM1/c-FOS pathway. Journal of translational medicine 24 33731128
2020 p53/PGC‑1α‑mediated mitochondrial dysfunction promotes PC3 prostate cancer cell apoptosis. Molecular medicine reports 24 32377739
2016 Effect of AQP9 Expression in Androgen-Independent Prostate Cancer Cell PC3. International journal of molecular sciences 24 27187384
2015 Antitumoral and Antioxidant Potential of Egyptian Propolis Against the PC3 Prostate Cancer Cell Line. Asian Pacific journal of cancer prevention : APJCP 24 26625775
2014 CBX8, a novel DNA repair protein, promotes tumorigenesis in human esophageal carcinoma. International journal of clinical and experimental pathology 24 25197352
2006 Effects of interactions of EGCG and Cd(2+) on the growth of PC-3 cells and their mechanisms. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 23 17046135
2015 Intracellular EP2 prostanoid receptor promotes cancer-related phenotypes in PC3 cells. Cellular and molecular life sciences : CMLS 22 25828575
1995 The role of prohormone convertases PC1 (PC3) and PC2 in the cell-specific processing of proglucagon. Biochemical and biophysical research communications 22 7864855
2020 Upregulated CBX8 Promotes Cancer Metastasis via the WNK2/MMP2 Pathway. Molecular therapy oncolytics 21 33251331
2008 Expression of nucleostemin in prostate cancer and its effect on the proliferation of PC-3 cells. Chinese medical journal 21 18304460
2008 Selective amino acid restriction differentially affects the motility and directionality of DU145 and PC3 prostate cancer cells. Journal of cellular physiology 21 18459146
2021 Pro-angiogenic effect of PC-3 exosomes in endothelial cells in vitro. Cellular signalling 20 34474113
2019 Chromobox homolog 8 (CBX8) Interacts with Y-Box binding protein 1 (YBX1) to promote cellular proliferation in hepatocellular carcinoma cells. Aging 20 31495785
2015 CBX8 antagonizes the effect of Sirtinol on premature senescence through the AKT-RB-E2F1 pathway in K562 leukemia cells. Biochemical and biophysical research communications 20 26718407
1994 Expression of PC2 and PC1/PC3 in human pheochromocytomas. Molecular and cellular endocrinology 20 8206338
2017 In Vitro Incorporation of Radioiodinated Eugenol on Adenocarcinoma Cell Lines (Caco2, MCF7, and PC3). Cancer biotherapy & radiopharmaceuticals 19 28358602
2016 Enhanced Cytotoxicity of Biomolecules Loaded Metallic Silver Nanoparticles Against Human Liver (HepG2) and Prostate (PC3) Cancer Cell Lines. Journal of nanoscience and nanotechnology 19 27483851
2009 TMF/ARA160 downregulates proangiogenic genes and attenuates the progression of PC3 xenografts. International journal of cancer 19 19330832
2019 Extracellular Electrophysiology in the Prostate Cancer Cell Model PC-3. Sensors (Basel, Switzerland) 18 30609788
2019 CBX8 promotes tumorigenesis and confers radioresistance in esophageal squamous cell carcinoma cells through targeting APAF1. Gene 18 31255735
2009 Skp2 enhances polyubiquitination and degradation of TIS21/BTG2/PC3, tumor suppressor protein, at the downstream of FoxM1. Experimental cell research 18 19615363
2006 Inhibition of caveolin-1 expression by incadronate in PC-3 prostate cells. Anticancer research 18 16886623
2005 The neurogene BTG2TIS21/PC3 is transactivated by DeltaNp73alpha via p53 specifically in neuroblastoma cells. Journal of cell science 18 15741235
2021 Investigation of molecular mechanisms underlying the antiproliferative effects of colchicine against PC3 prostate cancer cells. Toxicology in vitro : an international journal published in association with BIBRA 17 33684465
2019 Calcilytics inhibit the proliferation and migration of human prostate cancer PC-3 cells. Journal of pharmacological sciences 17 30808588
2018 Inhibition of neddylation facilitates cell migration through enhanced phosphorylation of caveolin-1 in PC3 and U373MG cells. BMC cancer 17 29301501
2016 Sulforaphane-cysteine suppresses invasion via downregulation of galectin-1 in human prostate cancer DU145 and PC3 cells. Oncology reports 17 27430422
2015 Inhibition of Transient Receptor Potential Melastain 7 Enhances Apoptosis Induced by TRAIL in PC-3 cells. Asian Pacific journal of cancer prevention : APJCP 17 26028116
2012 Implications of pleiotrophin in human PC3 prostate cancer cell growth in vivo. Cancer science 17 22783964
2021 Enhanced cadmium removal by growing Bacillus cereus RC-1 immobilized on different magnetic biochars through simultaneous adsorption and bioaccumulation. Environmental science and pollution research international 16 34689298
2016 A Novel Role of Chromodomain Protein CBX8 in DNA Damage Response. The Journal of biological chemistry 16 27555324
2015 Insulin-Like Growth Factor-1 Modulates Polycomb Cbx8 Expression and Inhibits Colon Cancer Cell Apoptosis. Cell biochemistry and biophysics 16 25398592
2006 Safrole-induced Ca2+ mobilization and cytotoxicity in human PC3 prostate cancer cells. Journal of receptor and signal transduction research 16 16777715
2020 Induction of proliferative and mutagenic activity by benzo(a)pyrene in PC-3 cells via JAK2/STAT3 pathway. Mutation research 15 32841893
2019 Trichostatin A inhibits proliferation of PC3 prostate cancer cells by disrupting the EGFR pathway. Oncology letters 15 31289542
2018 RNA interference-mediated depletion of TRPM8 enhances the efficacy of epirubicin chemotherapy in prostate cancer LNCaP and PC3 cells. Oncology letters 15 29541177
1994 Expression patterns of messenger ribonucleic acids encoding prohormone convertases (PC2 and PC3) in human pituitary adenomas. Clinical endocrinology 15 7923822
2022 Saffron extract feed improves the antioxidant status of laying hens and the inhibitory effect on cancer cells (PC3 and MCF7) Growth. Veterinary medicine and science 14 36063535
2019 Glycyrrhiza glabra-Enhanced Extract and Adriamycin Antiproliferative Effect on PC-3 Prostate Cancer Cells. Nutrition and cancer 14 31274029
2017 Effect of silencing the T‑Box transcription factor TBX2 in prostate cancer PC3 and LNCaP cells. Molecular medicine reports 14 28849151
2011 Downregulation of Id1 by small interfering RNA in prostate cancer PC3 cells in vivo and in vitro. European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP) 14 20881502
2005 Incadronate inhibits aminopeptidase N expression in prostatic PC-3 cells. Cancer letters 14 16019130
2018 PROSTAGLANDIN E2 stimulates cancer-related phenotypes in prostate cancer PC3 cells through cyclooxygenase-2. Journal of cellular physiology 13 30367494
2016 l-2-Haloacid dehalogenase (DehL) from Rhizobium sp. RC1. SpringerPlus 13 27347470
2013 TIS21(/BTG2/PC3) inhibits interleukin-6 expression via downregulation of STAT3 pathway. Cellular signalling 13 23917204