| 2011 |
RNF20 localizes to DNA double-strand breaks (DSBs) independently of H2AX and is required for DSB-induced H2B ubiquitination at K120, H3K4 methylation at DSBs, and recruitment of chromatin-remodeling factor SNF2h; depletion of RNF20 or expression of H2B K120R mutant compromises DNA end resection and recruitment of RAD51 and BRCA1, causing homologous recombination repair defects and radiation sensitivity. |
RNAi knockdown, H2B K120R mutant expression, immunofluorescence/foci analysis, HR repair assays, chromatin relaxation rescue experiments |
Molecular cell |
High |
21362548
|
| 2011 |
WAC interacts with RNF20/40 through its C-terminal coiled-coil region, recognizes RNA polymerase II via its N-terminal WW domain, and targets RNF20/40 to the RNA polymerase II complex at active transcription sites to promote H2B ubiquitination; WAC depletion abolishes H2B ubiquitination. |
Protein affinity purification, co-immunoprecipitation, RNAi knockdown, H2B ubiquitination assays, cell-cycle checkpoint assays |
Molecular cell |
High |
21329877
|
| 2012 |
Smurf2 (HECT-domain E3 ligase) targets RNF20 for proteasomal degradation, thereby regulating downstream H2B monoubiquitination and H3K4/H3K79 trimethylation; Smurf2 and RNF20 co-localize at γ-H2AX foci of DSBs. |
Genetic ablation (Smurf2 knockout mice), protein degradation assays, co-localization by immunofluorescence, western blot of histone marks |
Nature medicine |
High |
22231558
|
| 2012 |
RNF20 (BRE1A/Rnf20) deficiency causes replication-associated DSBs through formation of aberrant R-loops (RNA:DNA hybrids), leading to specific genomic rearrangements, breakage-fusion-bridge cycles, and chromosomal instability; this is compounded by a previously known HR repair defect. |
Bre1-deficient cell lines, genomic instability tracking, R-loop detection, DNA content analysis, cancer tissue comparison |
Cancer research |
Medium |
22354749
|
| 2012 |
RNF20-catalyzed H2B monoubiquitylation (H2Bub1) increases during embryonic stem cell differentiation and is required for efficient ESC differentiation, particularly for transcriptional induction of relatively long genes; USP44 deubiquitinase negatively regulates H2Bub1 and its downregulation during differentiation contributes to the H2Bub1 increase. |
ESC differentiation assays, RNAi knockdown of RNF20 and USP44, ChIP, gene expression analysis |
Molecular cell |
High |
22681888
|
| 2011 |
CDC73 (parafibromin) tumor suppressor interacts with RNF20 and RNF40 at discrete residues, and is required for maintenance of H2B K120 monoubiquitination; loss of nuclear CDC73 or CDC73 mutations (as in parathyroid carcinoma) significantly reduces H2Bub1 without affecting H3K4me3. |
Yeast two-hybrid, co-immunoprecipitation, siRNA knockdown, western blot of histone marks in parathyroid tumor tissue |
Human molecular genetics |
Medium |
22021426
|
| 2013 |
Histone chaperone FACT (SUPT16H subunit) directly binds RNF20 in vivo; mutation of the RNF20 RING-finger domain abolishes FACT-RNF20 interaction and RNF20 accumulation at DSB sites; SUPT16H depletion impairs RNF20-mediated H2B ubiquitylation and SNF2h recruitment at DSBs, causing HR repair defects; this interaction is PAF1-independent for DNA damage contexts. |
Co-immunoprecipitation, RING-domain mutagenesis, RNAi knockdown, laser-induced DSB foci analysis, HR repair assays, radiation sensitivity |
Journal of cell science |
High |
24357716
|
| 2013 |
RNF20 (hBRE1) is targeted by adenovirus E1A, which repurposes RNF20 from a ubiquitin ligase into a scaffold that recruits hPaf1 for transcriptional activation of viral early genes, simultaneously blocking H2B monoubiquitination required for the interferon response. |
Co-immunoprecipitation, transcription reporter assays, viral infection/replication assays, E1A interaction mapping |
PLoS pathogens |
Medium |
23785282
|
| 2013 |
RNF20 (histone H2B E3 ubiquitin ligase) is required for MLL-fusion-mediated leukemogenesis; H2B ubiquitination is enriched in the body of MLL-fusion target genes and correlates with H3K79 methylation; RNF20 is required to maintain local H3K79 methylation by DOT1L at Hoxa9 and Meis1, supporting MLL-fusion oncogenic transcriptional program. |
shRNA knockdown in leukemia models (in vitro and in vivo), ChIP-seq (H2Bub, H3K79me2), gene expression analysis |
Proceedings of the National Academy of Sciences of the United States of America |
High |
23412334
|
| 2014 |
Arsenite directly binds to the cysteine residues in the RING finger domains of RNF20 and RNF40 in vitro and in cells, inhibiting H2B ubiquitination and impairing BRCA1 and RAD51 recruitment to DSB sites, thereby compromising DNA DSB repair and rendering cells sensitive to radiomimetic agents. |
In vitro binding assays, cellular H2B ubiquitination assays, laser-induced DSB foci analysis, DNA repair assays, cell viability assays |
Journal of the American Chemical Society |
High |
25170678
|
| 2016 |
RNF20/RNF40 complex interacts with motor protein Eg5 during mitosis, monoubiquitinates and stabilizes Eg5; loss of RNF20/40 causes spindle assembly defects, cell cycle arrest, and apoptosis; this spindle assembly role is distinct from the transcriptional H2B ubiquitination function. |
Co-immunoprecipitation, ubiquitination assays, spindle assembly imaging, cell cycle analysis, in vivo tumor suppression assays |
Nature communications |
High |
27557628
|
| 2016 |
RNF20/RNF40 downregulation favors recruitment of p65-containing NF-κB dimers over repressive p50 homodimers and decreases H3K9me3 on a subset of NF-κB target genes, augmenting their transcription; RNF20+/- mice show predisposition to colonic inflammation and inflammation-associated colorectal cancer with excessive MDSCs. |
RNAi knockdown, ChIP, NF-κB dimer recruitment analysis, RNF20 heterozygous mouse model, colitis and cancer models |
Cell reports |
High |
26854224
|
| 2016 |
Crystal structure of the RNF20 RING domain reveals it forms a homodimer and specifically interacts with the Ube2B~Ub conjugate; key E3-E2 and E3-ubiquitin interface contacts were identified by mutagenesis; RING domains of RNF20 and RNF40 form a stable active heterodimer; structural model of RNF20-Ube2B~Ub complex generated. |
X-ray crystallography of RNF20 RING domain, site-directed mutagenesis of E3-E2 and E3-ubiquitin interfaces, in vitro ubiquitination assays |
Journal of molecular biology |
High |
27569044
|
| 2019 |
RNF20/40, in conjunction with its cognate E2 enzyme RAD6, monoubiquitylates lysine 381 of eEF1BδL (a heat shock transcription factor); this monoubiquitylation increases eEF1BδL accumulation and potentiates recruitment of p-TEFb to promoters of heat shock-responsive genes, enhancing their transcription; cooperative physical interactions among eEF1BδL, RNF20/40, and HSF1 synergistically promote heat shock gene expression. |
Biochemically defined in vitro ubiquitylation assays with purified factors, cell-based analyses, mass spectrometry identification of ubiquitylation site, ChIP, gene expression assays |
Nucleic acids research |
High |
30649429
|
| 2019 |
The RNF20/40 complex associates with p53 on chromatin; p53 mediates recruitment of RNF20/40 to p21 and PUMA gene loci; RNF20/40-dependent H2B ubiquitination (ubH2B) is required for transcription and mRNA splicing of p21 and PUMA; ubH2B is recognized by the ubiquitin-binding motif of pre-mRNA splicing factor PRPF8 (a spliceosome subunit), which is required for mRNA maturation. |
Protein affinity purification, co-immunoprecipitation, ChIP, RNAi knockdown, mRNA splicing assays, ubiquitin-binding domain interaction studies |
Journal of molecular cell biology |
High |
31152661
|
| 2019 |
RNF20 promotes proteasomal degradation of nuclear corepressor 1 (NCoR1) via ubiquitination, which relieves NCoR1-mediated repression and stimulates PPARγ transcriptional activity in adipocytes, thereby promoting adipogenesis; Rnf20 conditional knockout mice show reduced fat mass and altered PPARγ target gene expression. |
Conditional knockout mouse model, quantitative proteomics, co-immunoprecipitation, ubiquitination/proteasomal degradation assays |
Diabetes |
High |
31604693
|
| 2020 |
The SARS-CoV-2 main protease 3Clpro cleaves RNF20 at a conserved Gln521 in a catalytically dependent manner (catalytic mutant 3ClproC145A does not cleave); cleavage of RNF20 prevents RNF20-mediated degradation of SREBP1, promoting viral replication; RNAi depletion of RNF20 or RNF40 significantly enhances SARS-CoV-2 replication. |
Protease cleavage assays with catalytic mutant control, RNAi knockdown, SARS-CoV-2 replication assays, SREBP1 protein stability assays |
Proceedings of the National Academy of Sciences of the United States of America |
High |
34452991
|
| 2017 |
RNF20 acts as a tumor suppressor in clear cell renal cell carcinoma by promoting degradation of SREBP1c, which represses lipogenesis and cell cycle progression through the PTTG1 oncogene (a novel SREBP1c target); RNF20 overexpression reduces tumor growth and lipid storage in xenografts. |
RNF20 overexpression and knockdown, cell proliferation assays, xenograft tumor models, lipogenesis assays, ChIP for SREBP1c at PTTG1 promoter |
Molecular and cellular biology |
Medium |
28827316
|
| 2010 |
RNF20 (hBRE1) and MEN1 are recruited to the IRF1 gene locus in both uninduced and IFNγ-induced states; RNAi-mediated depletion of RNF20 lowers H2Bub and H3K4me3 at IRF1, but unexpectedly upregulates IFNγ-induced IRF1 transcription, indicating that RNF20 can be repressive of inducible transcription at this locus. |
ChIP, RNAi knockdown, RT-PCR gene expression analysis, chemical inhibition of H3K4 methylation |
Epigenetics & chromatin |
Medium |
20825659
|
| 2013 |
RNF20 promotes polyubiquitination and proteasome-dependent degradation of transcription factor AP-2α; RNF20 co-localizes and interacts with AP-2α in preadipocytes; RNF20 overexpression rescues C/EBPα expression suppressed by AP-2α. |
Co-immunoprecipitation, mass spectrometry, ubiquitination assays, proteasome inhibition, co-localization microscopy, C/EBPα expression rescue assays |
Acta biochimica et biophysica Sinica |
Medium |
24374663
|
| 2017 |
Fbxl19, a CxxC domain-containing protein, promotes H2Bub1 at CpG island-containing gene promoters by directly interacting with Rnf20; Fbxl19 preferentially occupies CpG island-containing promoters genome-wide and its chromosomal binding is required for H2Bub1 at those targets; Fbxl19 is critical for proper ESC differentiation in collaboration with Rnf20. |
Co-immunoprecipitation, genome-wide ChIP-seq (Fbxl19, H2Bub1), RNAi knockdown of Fbxl19, ESC differentiation assays |
Nucleic acids research |
Medium |
28453857
|
| 2019 |
RNF20 and RNF40 are required for DSB repair leading to both homologous recombination and class switch recombination (NHEJ-driven) in mouse B cells; DSBs induce a global increase in H2Bub but not H3K4me3 or H3K79me2; H2AX phosphorylation is dispensable for H2Bub; ATM and ATR jointly regulate IR-induced H2Bub. |
RNAi knockdown in mouse B cells, class switch recombination assays, HR assays, western blot of histone marks, kinase inhibitor treatments |
Molecular and cellular biology |
High |
30692271
|
| 2023 |
Cryo-EM structures of chemically-trapped transient ubiquitin transfer complexes reveal that RNF40 (and yeast Bre1) directly bind nucleosomal DNA; the E3/E2/nucleosome interaction pattern is conserved from yeast to humans; Bre1 RING domain uses a non-canonical non-hydrophobic contact with Rad6 to position E2 directly above target H2B lysine; these structures explain site-specific H2B monoubiquitylation mechanism. |
Chemical trapping, cryo-electron microscopy structure determination, biochemical reconstitution, cancer mutation mapping |
Molecular cell |
High |
37633270
|
| 2023 |
RPA (single-strand DNA-binding factor) interacts with RNF20 mainly in S and G2/M phases and recruits RNF20 to mitotic centromeres in a centromeric R-loop-dependent manner and to chromosomal breaks upon DNA damage; the RPA-RNF20 pathway promotes local H2Bub, H3K4 dimethylation, and SNF2H recruitment, ensuring proper Aurora B kinase activation at centromeres and efficient loading of repair proteins at DSBs. |
Co-immunoprecipitation, ChIP, cell cycle analysis, mitotic phenotype assays (lagging chromosomes, chromosome bridges), HR repair assays, R-loop disruption experiments |
Proceedings of the National Academy of Sciences of the United States of America |
High |
37155876
|
| 2023 |
Yeast Bre1 and human RNF20 function as recombination mediators independently of their E3 ligase activity: they interact with Rad51, direct Rad51 to ssDNA, facilitate Rad51-ssDNA filament assembly and strand exchange in vitro, and interact with Srs2/FBH1 helicases to counteract their disruption of Rad51 filaments; this mediator function is additive with Rad52 (yeast) or BRCA2 (human). |
In vitro Rad51 filament assembly and strand exchange assays, co-immunoprecipitation, ligase-dead mutant analysis, genetic epistasis with rad52/BRCA2, HR assays in cells |
Nature communications |
High |
37230987
|
| 2021 |
RNF20 and RNF40 form a complex that monoubiquitinates H2B on lysine 120; in vivo CRISPR screen in mouse cardiomyocytes identified RNF20/40-mediated H2Bub as controlling dynamic changes in gene expression required for cardiomyocyte maturation. |
In vivo somatic Cas9 mutagenesis (forward genetic screen in mice), cardiomyocyte maturation phenotyping, gene expression analysis |
Nature communications |
Medium |
34290256
|
| 2018 |
RNF20 (spinal) mediates H2B monoubiquitination in dorsal horn neurons following spinal nerve ligation; RNF20-dependent H2Bub facilitates phosphorylated RNA polymerase II-dependent mGluR5 transcription; focal knockdown of spinal RNF20 reverses neuropathic allodynia and reduces mGluR5 expression; TNF-α activates this RNF20/H2Bub/RNAPII transcriptional axis. |
Spinal nerve ligation rat model, RNAi knockdown in vivo, ChIP of H2Bub and phospho-RNAPII at mGluR5 promoter, behavioral allodynia assays, neutralizing antibody experiments |
The Journal of neuroscience |
Medium |
30201771
|
| 2024 |
RNF20 stabilizes LSD1 via K29-linked ubiquitination in a CDK9-phosphorylation-dependent manner; this CDK9-RNF20-LSD1 axis is required for H3K4 demethylation, repression of endogenous retroviruses, and suppression of interferon response, thereby contributing to epigenetic immunosuppression; loss of RNF20 sensitizes cancer cells to anti-PD-1 immunotherapy in vivo. |
Co-immunoprecipitation, ubiquitination assays (K29-specific), CDK9 inhibition/phosphorylation analysis, endogenous retrovirus expression, in vivo anti-PD-1 rescue experiment, breast cancer specimen correlation |
Proceedings of the National Academy of Sciences of the United States of America |
High |
38315842
|
| 2024 |
RNF20 controls Rbx1 expression, thereby regulating activity of the VHL ubiquitin ligase complex and HIF1α protein levels; Rnf20 haploinsufficiency increases HIF1α levels and drives metabolic rewiring and EMT through HIF1α-mediated RNAPII promoter-proximal pause release independently of H2Bub1; this links the DNA damage response and metabolic reprogramming in lung cancer. |
Rnf20 haploinsufficiency mouse lung tumor model, genetic ablation, gene expression analysis, metabolomics, HIF1α protein stability assays, Rbx1/VHL epistasis experiments |
Nature communications |
Medium |
40436847
|
| 2024 |
HIF-1 recruits FACT and RNF20/40 to hypoxia response elements (HREs); FACT and RNF20/40 stabilize HIF-1 binding and each other at HREs; hypoxia induces H2B monoubiquitination at HIF-1 target genes in an HIF-1-dependent manner; knockdown of FACT or RNF20/40 decreases transcription initiation and elongation at HIF-1 target genes. |
RNAi knockdown of FACT and RNF20/40, ChIP at HREs, H2Bub ChIP under hypoxia, transcription initiation/elongation analysis in breast cancer cells |
Cell reports |
Medium |
38517892
|
| 2024 |
RNF20 mediates NCoR1 protein degradation to activate PPARγ in iWAT for beige fat differentiation upon prolonged cold stimuli; in BAT, cold stimulus downregulates RNF20, which elevates GABPα protein stability (RNF20 controls GABPα stability), promoting thermogenic gene expression; thus RNF20 uses fat depot-specific substrates (GABPα in BAT, NCoR1 in iWAT) to orchestrate adipose thermogenesis. |
Fat depot-specific Rnf20 knockout mice, cold exposure experiments, protein stability assays for GABPα and NCoR1, gene expression analysis, ubiquitination assays |
Nature communications |
High |
38296968
|
| 2024 |
RNF20 regulates oocyte meiotic spindle assembly by localizing to centromeres and spindle poles and recruiting tropomyosin 3 (TPM3) via its coiled-coil motif (not its E3 ligase activity); RNF20-depleted oocytes show abnormal spindle organization and chromosome misalignment; the RNF20-TPM3 interaction is essential for acentrosomal meiotic spindle assembly. |
RNF20 depletion in mouse oocytes, E3 ligase-dead mutant analysis, co-immunoprecipitation of RNF20-TPM3, immunofluorescence of spindle/centromere localization, coiled-coil domain mapping |
Advanced science |
High |
38240347
|
| 2024 |
RNF20 mediates RNA polymerase II promoter-proximal pausing at highly paused endothelial genes involved in VEGFA signaling; RNF20 binds Notch1 to promote H2B monoubiquitination at Notch target genes and Notch-dependent gene expression; loss of Rnf20 leads to uncontrolled tip cell specification; RNF20 restricts ERG-dependent Pol II pause release at highly paused genes. |
Rnf20 knockout in endothelial cells, RNA Pol II ChIP-seq (pause analysis), Notch1 co-immunoprecipitation, H2Bub ChIP, angiogenesis/tip cell phenotype assays |
Nature cardiovascular research |
High |
39322771
|
| 2023 |
Iron deficiency-activated ferritinophagy induces RNF20 degradation through the autophagy-lysosomal pathway; RNF20 degradation negatively regulates H2B K120 monoubiquitination at promoters of myogenic markers MyoD and MyoG, inhibiting myogenic differentiation and muscle regeneration; overexpression of RNF20 or autophagy inhibition restores regeneration under iron-deficient conditions. |
Iron deficiency model in cells and mice, autophagy-lysosome pathway inhibition, RNF20 overexpression rescue, ChIP for H2Bub at myogenic promoters, conditional NCOA4 knockout in satellite cells |
Science advances |
High |
37976359
|
| 2025 |
RNF20 localizes to replicating sites and promotes H2Bub there; RNF20 knockdown leads to nucleolytic degradation of stalled replication forks (rescued by MRE11/DNA2 inhibition and depletion of fork remodelers SMARCAL1/HLTF/ZRANB3); RNF20 facilitates RAD51 and RAD51C loading at stalled forks, acting in the same pathway as RAD51/RAD51C-mediated fork protection; RNF20 RING domain and ATR-mediated phosphorylation are essential for its role in replication stress responses. |
RNF20 knockdown, DNA fiber assays (fork protection/restart), RING-domain mutant and phosphorylation-deficient mutant analysis, nuclease inhibition, fork remodeler co-depletion, RAD51/RAD51C loading assays |
EMBO reports |
High |
40495033
|
| 2021 |
RNF20 and RNF40 regulate Vitamin D Receptor (VDR) gene and VDR target gene transcription through H3K4me3 occupancy dependent on H2Bub1; conditional intestinal deletion of Rnf20 or Rnf40 in mice causes spontaneous colorectal inflammation; this mechanism is linked to IBD pathogenesis. |
Conditional intestinal Rnf20/Rnf40 knockout mice, ChIP-seq for H2Bub1 and H3K4me3 at VDR locus, mRNA-seq, IBD patient ChIP-seq |
Cell death and differentiation |
High |
34088983
|
| 2023 |
KSHV RTA protein interacts with the cellular RNF20/40 E3 ubiquitin ligase complex, and this interaction is necessary for RTA-induced KSHV lytic cycle reactivation. |
Proteomics identification of RTA-RNF20/40 interaction, functional lytic reactivation assays, co-immunoprecipitation validation |
Journal of virology |
Medium |
37888983
|
| 2018 |
RNF20 interacts with ZSCAN4 protein and negatively regulates ZSCAN4 stability; RNF20 depletion does not affect ZSCAN4 transcription but increases ZSCAN4 protein levels and stabilizes its half-life, indicating RNF20 promotes ZSCAN4 degradation at the protein level. |
Co-immunoprecipitation, protein half-life assays, RNAi knockdown of RNF20, western blot, RT-PCR |
Biochemical and biophysical research communications |
Medium |
29477841
|
| 2020 |
In breast cancer cells, Snail interacts with RNF20 and G9a; RNF20 and H3K9me2 (G9a mark) are enriched on the E-cadherin promoter in a Snail-dependent manner; RNF20-mediated H2BK120 monoubiquitination contributes to E-cadherin repression and EMT induction. |
Co-immunoprecipitation (Snail-RNF20-G9a), ChIP at E-cadherin promoter, RNF20 knockdown/overexpression, migration/invasion/tumorsphere assays |
Frontiers in oncology |
Medium |
33364200
|
| 2023 |
RNF20 promotes STAT3 ubiquitination and degradation; RNF20 is a target gene of exosomal miR-let-7b-5p (confirmed by luciferase reporter assay); ectopic RNF20 expression attenuates insulin resistance by downregulating STAT3 protein expression via ubiquitination-mediated degradation. |
Luciferase reporter assay (RNF20 as let-7b-5p target), IP/western blot for STAT3 ubiquitination, RNF20 overexpression rescue experiments |
Diabetes, metabolic syndrome and obesity |
Low |
37842335
|
| 2025 |
Crystal structure of Bre1-Lge1 complex and AlphaFold model of RNF20/RNF40-WAC reveal extensive interaction interfaces; the Bre1-Lge1 and RNF20/RNF40-WAC interfaces share structural homology but use completely different electrostatic interactions encoding binding specificity; these interactions are critical for the H2BUb1 reaction and processes it regulates. |
X-ray crystallography of Bre1-Lge1, AlphaFold modeling of RNF20/RNF40-WAC, interface mutagenesis, in vitro H2BUb1 assays, in vivo functional assays |
Nucleic acids research |
High |
41533567
|
| 2026 |
Cryo-EM structure of chemically-trapped RNF20/RNF40-RAD6A-Ub-H2BS112GlcNAc nucleosome complex shows that H2BS112GlcNAc interacts with E2 enzyme RAD6A (not RNF20/RNF40); GlcNAcylation allosterically stimulates ubiquitin transfer from RAD6A~Ub to H2B K120 by enhancing the nucleophilicity of H2B K120; the C2 N-acetyl group and β-configuration of C1 are essential for this activation. |
Chemical synthesis of H2BS112GlcNAc-modified nucleosomes, chemical trapping, cryo-EM structure determination, mutagenesis, kinetics analysis, structure-activity relationship |
Nature chemical biology |
High |
41495224
|
| 2023 |
RNF20 is required for male fertility through regulation of H2BK120 ubiquitination in Sertoli cells; Sertoli cell-specific Rnf20 knockout mice are infertile due to spermatogenic failure mimicking Sertoli cell-only syndrome; RNF20 deficiency impairs transcription elongation of Cldn11 (encoding tight junction component claudin-11), disrupts cell adhesion and seminiferous tubule organization, and leads to apoptosis of spermatogonia and spermatocytes. |
Conditional Sertoli cell-specific Rnf20 knockout mice (Amh-Cre), histology, H2Bub1 western blot, Cldn11 expression analysis, ChIP for H2Bub at Cldn11 locus |
Cell & bioscience |
High |
37024990
|
| 2025 |
In β-cells, Rnf20 forms complexes with the Isl1 transcription factor; conditional Rnf20 knockout in adult β-cells causes severe hyperglycemia, loss of H2B monoubiquitination, dysregulation of glucose-stimulated insulin secretion, and disruption of β-cell identity gene expression; comparative analysis shows Isl1 and Rnf20 loss yield similar β-cell regulome changes; Isl1::Rnf20 complexes regulate insulin expression and secretion in human tissues. |
β-cell-specific conditional Rnf20 knockout (tamoxifen-inducible), co-immunoprecipitation of Isl1-Rnf20, H2Bub1 western blot, transcriptomic analysis, glucose tolerance tests, insulin secretion assays |
Diabetes |
High |
40743566
|
| 2025 |
RNF20 dynamically translocates between nucleus and cytoplasm in response to RNA virus infection (export via CRM1); in resting and early infection states, nuclear RNF20 maintains basal and inducible transcription of RIG-I and MDA5; upon late viral infection, cytoplasmic RNF20 recognizes degron motifs of RIG-I and MDA5 via its coiled-coil domain and catalyzes K27-linked ubiquitination and degradation of both sensors, preventing excessive antiviral signaling. |
RNF20 overexpression/knockout (in vitro and in vivo), co-immunoprecipitation, ubiquitination assays (K27-specific), CRM1 export inhibition, transcriptome sequencing, viral infection assays |
PLoS pathogens |
Medium |
41712649
|
| 2022 |
The natural compound epoxymicheliolide (ECL) covalently modifies H2B lysine 46 (K46) and recruits E3 ubiquitin ligase RNF20 to promote H2Bub1 at K120; ECL-mediated H2Bub1 disrupts AP-1 recruitment to proinflammatory gene promoters, inhibiting microglia-mediated neuroinflammation. |
Biochemical binding assays, ChIP-seq, transcriptomics, site-specific mutagenesis of H2B K46, in vitro and in vivo neuroinflammation models |
Pharmacological research |
Medium |
35074526
|
| 2014 |
Bre1a (RNF20 mouse ortholog) regulates neural precursor cell (NPC) differentiation and cell cycle length; knockdown of Bre1a in NPCs lengthens cell cycle through upregulation of p57kip2 and downregulation of Cdk2, and increases Hes5 expression via Fezf1 and Fezf2 to suppress NPC differentiation. |
RNAi knockdown in embryonic mouse NPCs, cell cycle analysis, RT-PCR for p57kip2/Cdk2/Hes5/Fezf1/Fezf2, differentiation assays |
The Journal of neuroscience |
Medium |
24553946
|
| 2025 |
RNF20/RNF40, SET1/COMPASS, and DOT1L are dispensable for transcription restart after DNA damage repair (negative finding); levels of H2B-K120Ub and H3K4me3 do not correlate with transcription restoration following DNA damage; PAF1C promotes transcription restart independently of H2B-K120Ub deposition. |
RNF20/RNF40 depletion, SET1/COMPASS and DOT1L inhibition, transcription restart assays after DNA damage, ChIP for H2Bub/H3K4me3 |
bioRxivpreprint |
Medium |
|