Affinage

BRD7

Bromodomain-containing protein 7 · UniProt Q9NPI1

Length
651 aa
Mass
74.1 kDa
Annotated
2026-06-09
97 papers in source corpus 41 papers cited in narrative 40 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

BRD7 is a bromodomain-containing subunit of the PBAF-specific SWI/SNF chromatin-remodeling complex that acts as a transcriptional co-regulator and tumor suppressor by coupling acetylated-chromatin recognition to the activity of sequence-specific regulators (PMID:18809673, PMID:16265664). Its bromodomain adopts a four-helix bundle that binds acetylated lysines on histone H3 and H4 (H3K9ac, H3K14ac, H4K8/12/16ac), an interaction required for its transcriptional control of cell-cycle genes such as E2F3 and for G1-S arrest (PMID:16265664, PMID:17498659); nuclear function depends on a bipartite NLS, and loss of nuclear localization abolishes cell-cycle regulation (PMID:16475162). BRD7 physically interacts with p53 and p300, is recruited to p53 target promoters, and is required for efficient p53-dependent transcription and oncogene-induced senescence (PMID:20228809, PMID:20660729); it further stabilizes p53 by blocking AKT-dependent MDM2 phosphorylation and p53 ubiquitination through its bromodomain (PMID:35371302), and under ferroptotic conditions binds the p53 transactivation domain to drive p53 mitochondrial translocation and SLC25A28-dependent iron accumulation (PMID:32863216). It also directly binds BRCA1 to co-regulate a subset of target genes including ESR1/ERα (PMID:20215511) and engages the PI3K regulatory subunits p85α/β, competing with p110 and driving nuclear translocation of p85 and XBP1s to restrain PI3K/AKT signaling and support the unfolded protein response and glucose homeostasis (PMID:24657164, PMID:24836559). In the DNA damage response, ATM-phosphorylated BRD7 is recruited to double-strand breaks where it joins PRC2 and NuRD and recruits RNF168 to couple local transcriptional repression with repair (PMID:33101843). BRD7 protein levels are controlled by multiple ubiquitin-dependent routes, including PARP1/RNF146 PARylation-coupled degradation (PMID:30940648), TRIM28- and TRIM25-mediated K48 ubiquitination at K21 and K119 (PMID:39222175, PMID:41315221), and APC/C-Cdh1/Cdc20 cell-cycle degradation (PMID:24840027). In immunity, BRD7 bridges BRG1 and PBRM1 to nucleate PBAF assembly, increasing chromatin accessibility at Tbx21 to drive CD8+ T cell effector differentiation (PMID:38954484), and maintains metastatic dormancy by sustaining MHC-1 expression and tumor immunosurveillance (PMID:39910049).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 2000 Low

    Early interaction screens placed BRD7 in the nucleus in association with transcriptional regulators, establishing it as a candidate chromatin-associated co-regulator before its complex membership was known.

    Evidence Yeast two-hybrid and GST pull-down with IRF-2, plus PTP-BL PDZ-domain interaction, with immunofluorescence showing nuclear co-localization with active chromatin marks

    PMID:10526152 PMID:11025449

    Open questions at the time
    • Functional consequence of the IRF-2 and PTP-BL interactions not established
    • No demonstration of a chromatin-remodeling role at this stage
  2. 2003 High

    BRD7 was shown to modulate transcription through partner proteins and direct histone contact, framing it as a bromodomain reader that tunes activator/repressor balance.

    Evidence Co-IP, GST pull-down and reporter assays with E1B-AP5/hnRNP and Dvl-1, including a BRD7-histone triple complex and Wnt/β-catenin readouts

    PMID:12489984 PMID:12941796

    Open questions at the time
    • Specificity of histone-mark recognition not resolved
    • Mechanism linking BRD7 to β-catenin nuclear translocation incompletely defined
  3. 2006 Medium

    The bromodomain and an NLS were defined as the molecular determinants of BRD7 chromatin function and cell-cycle control, answering how BRD7 engages chromatin to regulate proliferation.

    Evidence Histone peptide pull-down with bromodomain-deletion mutant, GFP-NLS deletion constructs, reporter assays and flow cytometry on E2F3/cyclin D1 and G1-S progression

    PMID:16265664 PMID:16475162

    Open questions at the time
    • In vitro mark preference not yet structurally explained
    • Single-lab functional readouts
  4. 2007 High

    A structural model of the BRD7 bromodomain explained its acetyl-lysine reader fold while revealing low intrinsic mark selectivity, implying targeting specificity arises from complex context.

    Evidence NMR structure determination and titration with H3/H4 acetyl-lysine peptides

    PMID:17498659

    Open questions at the time
    • Does not address in-vivo mark selectivity within PBAF
    • No co-structure with histone or other subunits
  5. 2008 High

    BRD7 was defined biochemically as a PBAF-specific SWI/SNF subunit, distinguishing it from BAF and establishing its remodeling-complex identity.

    Evidence MS-based complex purification, shRNA knockdown of BRD7 vs ARID1A, gene-expression profiling and PIC recruitment assays; also BRD2 and Ceap-16/BLOS2 interactions mapped

    PMID:16786191 PMID:18329849 PMID:18809673

    Open questions at the time
    • How BRD7 directs PBAF to specific loci not fully defined
    • Functional roles of BRD2 and Ceap-16 interactions underexplored
  6. 2010 High

    BRD7 was established as a required co-regulator for p53 and BRCA1 transcriptional programs, defining its tumor-suppressor function at target promoters.

    Evidence Reciprocal Co-IP, ChIP, reporter and senescence assays for p53/p300; yeast two-hybrid and endogenous Co-IP with BRCA1, ESR1 promoter ChIP

    PMID:20215511 PMID:20228809 PMID:20660729

    Open questions at the time
    • Which target subsets require BRD7 versus the broader PBAF complex unclear
    • Direct versus complex-mediated recruitment not fully separated
  7. 2014 High

    BRD7 was shown to restrain PI3K/AKT signaling and regulate the UPR by sequestering and relocalizing p85 subunits, extending its role beyond chromatin to cytoplasmic signaling and metabolism.

    Evidence Co-IP with domain mapping, RNAi, AKT phosphorylation and fractionation assays for p85α; in vivo adenoviral BRD7 rescue in obese mice with XBP1s nuclear translocation and glucose readouts; APC/C-mediated degradation defined

    PMID:24657164 PMID:24836559 PMID:24840027

    Open questions at the time
    • Balance between nuclear chromatin and cytoplasmic p85-sequestering functions unresolved
    • How metabolic and tumor-suppressor roles are coordinated unclear
  8. 2019 High

    BRD7 stability was shown to be controlled by PARylation-coupled ubiquitination, explaining how chemotherapy-induced DNA damage lowers BRD7 levels.

    Evidence Co-IP, PARylation and ubiquitination assays, RNF146 knockdown and protein-stability western blots

    PMID:30940648

    Open questions at the time
    • Functional consequence of BRD7 loss for chemoresponse not fully defined here
    • Other PAR-dependent ligases not excluded
  9. 2020 High

    BRD7 was integrated into the DNA damage response and into protein-stability control of metastasis drivers, broadening its mechanistic reach in cancer.

    Evidence ATM-phosphorylation mapping and Co-IP with PRC2/NuRD/RNF168 at DSBs; ferroptosis Co-IP with p53 TAD and SLC25A28; YB1 phospho-degradation and metastasis assays; HIF1α degradation and glycolysis assays

    PMID:29725006 PMID:32028981 PMID:32863216 PMID:33101843

    Open questions at the time
    • Hierarchy among these many BRD7 protein-stability targets unclear
    • Cell-type specificity of each axis not systematically compared
  10. 2024 High

    BRD7 was defined as the bridging factor that nucleates PBAF assembly during CD8+ T cell activation, connecting its chromatin-remodeling role to adaptive immunity.

    Evidence Conditional CD8+ T cell knockout, Co-IP of BRG1/PBRM1, ATAC-seq at Tbx21, and viral infection models; TRIM28 K21 ubiquitination also defined

    PMID:38954484 PMID:39222175

    Open questions at the time
    • How BRD7-driven PBAF selection generalizes beyond the Tbx21 locus unknown
    • Relationship between immune and tumor-suppressor functions undefined
  11. 2025 High

    BRD7 was identified as a restraint on metastatic dormancy release through maintenance of MHC-1 and tumor immunosurveillance, linking its PBAF function to anti-metastatic immunity.

    Evidence In vivo PBAF subunit screen with single-cell RNA-seq, epigenomic profiling, neutrophil depletion, NET inhibition and immune checkpoint therapy; TRIM25 K119 ubiquitination axis defined

    PMID:39910049 PMID:41315221

    Open questions at the time
    • Direct chromatin targets controlling MHC-1 not pinpointed
    • Whether the immune phenotype is cell-intrinsic or microenvironment-driven not fully separated

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unknown how BRD7's locus-specific PBAF targeting, its many cytoplasmic protein-stability functions, and its tissue-specific phenotypes are mechanistically unified under a single regulatory logic.
  • No structural model of BRD7 within assembled PBAF on chromatin
  • No integrated model reconciling nuclear chromatin versus cytoplasmic signaling roles
  • Genome-wide direct BRD7 binding map not established

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 4 GO:0003677 DNA binding 3 GO:0042393 histone binding 3 GO:0060090 molecular adaptor activity 2 GO:0098772 molecular function regulator activity 2
Localization
GO:0005634 nucleus 3 GO:0005654 nucleoplasm 3 GO:0005829 cytosol 2
Pathway
R-HSA-162582 Signal Transduction 3 R-HSA-1640170 Cell Cycle 3 R-HSA-392499 Metabolism of proteins 3 R-HSA-168256 Immune System 2 R-HSA-4839726 Chromatin organization 2 R-HSA-5357801 Programmed Cell Death 2 R-HSA-73894 DNA Repair 2 R-HSA-74160 Gene expression (Transcription) 2
Partners
BRCA1BRG1EZH2HIF1APBRM1PIK3R1TP53YBX1
Complex memberships
PBAF (PBAF-specific SWI/SNF complex)

Evidence

Reading pass · 40 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2008 BRD7 is a novel subunit of the PBAF (Polybromo-associated BRG1-associated factor) SWI/SNF chromatin-remodeling complex, distinct from the BAF complex; knockdown of BRD7 (PBAF-specific) and ARID1A (BAF-specific) differentially and sometimes antagonistically regulate SWI/SNF target genes, and SWI/SNF affects recruitment of preinitiation complex components at target promoters. Mass spectrometry-based complex purification, shRNA knockdown, gene expression profiling, preinitiation complex recruitment assays The Journal of biological chemistry High 18809673
2010 BRD7 physically interacts with p53 and p300, is recruited to p53 target gene promoters, and is required for efficient p53-mediated transcription of a subset of target genes; BRD7 affects histone acetylation and p53 acetylation at these promoters, supporting oncogene-induced senescence. Co-immunoprecipitation, ChIP, shRNA knockdown, luciferase reporter assays, senescence assays Nature cell biology High 20228809 20660729
2010 BRD7 directly binds BRCA1 (identified by yeast two-hybrid, confirmed by co-immunoprecipitation of endogenous proteins), and is required for BRCA1-mediated transcriptional regulation; BRD7 depletion prevents recruitment of BRCA1 and Oct-1 to the ESR1 promoter, reducing ERα expression, without affecting recruitment of other SWI/SNF subunits (BRG1, BAF155, BAF57) or RNA Pol II. Yeast two-hybrid, endogenous co-immunoprecipitation, ChIP, microarray expression profiling, shRNA knockdown Cancer research High 20215511
2006 BRD7's bromodomain binds acetylated histone H3 (specifically H3K14ac), co-localizes with acetylated H3 in the nucleus, and this interaction is required for BRD7-mediated transcriptional regulation of E2F3 and inhibition of G1-S cell cycle progression; a bromodomain-deleted BRD7 mutant fails to bind acetylated H3 peptide and loses these functions. Co-localization immunofluorescence, histone peptide pull-down, bromodomain deletion mutant analysis, luciferase reporter assay, flow cytometry Journal of cellular biochemistry High 16265664
2007 NMR solution structure of the BRD7 bromodomain reveals a typical left-handed four-helix bundle topology; NMR titration experiments show it binds with weak affinity to acetylated lysine peptides from H3 (K9ac, K14ac) and H4 (K8ac, K12ac, K16ac), lacking inherent binding specificity among these marks in vitro. NMR spectroscopy (structure determination and titration) Biochemical and biophysical research communications High 17498659
2006 BRD7 contains a functional nuclear localization signal (NLS) spanning amino acids 65–96 composed of bipartite nuclear targeting sequences; deletion of the NLS shifts BRD7 localization from nucleus to cytoplasm and abolishes its ability to regulate cyclin D1, E2F3 expression, and G1-S cell cycle progression. GFP fusion constructs, deletion mutagenesis, fluorescence microscopy, western blot, flow cytometry Journal of cellular biochemistry Medium 16475162
2014 BRD7 interacts with the inter-SH2 (iSH2) domain of p85α (PI3K regulatory subunit) via a conserved C-terminal region, facilitates nuclear translocation of p85α, depletes cytosolic p85/p110 complexes, and thereby reduces p110 protein stability and PI3K/Akt pathway signaling; BRD7 and p110 compete for binding to p85. Co-immunoprecipitation, domain mapping, RNAi knockdown, Akt phosphorylation assays, subcellular fractionation Molecular cell High 24657164
2014 BRD7 interacts with regulatory subunits of PI3K (p85α and p85β) and promotes nuclear translocation of both p85 isoforms and the spliced form of XBP1 (XBP1s); BRD7 deficiency blocks nuclear translocation of XBP1s, impairing the unfolded protein response (UPR); restoring hepatic BRD7 in obese mice improves XBP1s nuclear translocation and glucose homeostasis. Co-immunoprecipitation, subcellular fractionation, in vivo mouse models (adenoviral BRD7 restoration), glucose tolerance tests Cell metabolism High 24836559
2003 BRD7 (Celtix-1/BP75) forms a complex with the heterogeneous nuclear ribonucleoprotein E1B-AP5 both in vivo and in vitro; disruption of this complex increases E1B-AP5 transcriptional repression activity and converts E1B-AP5 from an activator to a strong repressor of hormone-dependent promoters; BRD7 bromodomain binds histones H2A, H2B, H3, and H4, and a triple complex of E1B-AP5, BRD7, and histones was demonstrated. Yeast two-hybrid, in vivo and in vitro co-immunoprecipitation, GST pull-down, luciferase reporter assays, deletion mutagenesis The Biochemical journal High 12489984
2000 BRD7 (Celtix-1) interacts with IRF-2 via the C-terminus of IRF-2, as established by yeast two-hybrid and GST pull-down; a subset of BRD7/Celtix-1 co-localizes with hyperacetylated histones H3/H4 and the hyperphosphorylated (active) form of RNA Pol II in the nucleus. Yeast two-hybrid, GST pull-down, immunofluorescence co-localization Journal of cellular physiology Medium 11025449
1999 BP75 (BRD7) was identified through interaction with the first PDZ domain of protein tyrosine phosphatase PTP-BL in a yeast two-hybrid screen; both BP75 and a PTP-BL PDZ domain construct localize primarily to the nucleus. Yeast two-hybrid, subcellular localization by immunofluorescence FEBS letters Low 10526152
2003 BP75 (BRD7) directly interacts with Dvl-1 (Dishevelled-1), enhances TCF-dependent gene expression induced by Dvl-1, facilitates dephosphorylation of GSK-3β at Tyr216 thereby inhibiting its kinase activity, and synergizes with Dvl-1 to promote nuclear translocation of β-catenin. Yeast two-hybrid, co-immunoprecipitation in mammalian cells, TCF luciferase reporter assay, GSK-3β kinase assay, β-catenin localization Cancer research Medium 12941796
2009 BRD7 interacts with TRIM24/TIF1α and represses TRIM24-enhanced androgen receptor (AR) transactivation activity, as measured by luciferase reporter assay in prostate cancer cells. Co-immunoprecipitation, luciferase reporter assay Biochimica et biophysica acta Low 19909775
2004 Ectopic BRD7 expression in NPC cells inhibits G1-S progression and transcriptionally regulates molecules in the Ras/MEK/ERK and Rb/E2F pathways; BRD7 negatively regulates E2F3 promoter activity. Tet-on inducible expression, cell cycle cDNA array, luciferase reporter assay, flow cytometry Journal of cellular physiology Medium 15137061
2019 PARP-1 binds to BRD7 and poly(ADP-ribosyl)ates it; this modification enhances BRD7 ubiquitination and degradation through the PAR-binding E3 ubiquitin ligase RNF146, leading to BRD7 degradation in response to DNA damage-inducing chemotherapy. Co-immunoprecipitation, PAR assay (PARylation), ubiquitination assay, RNF146 knockdown, western blot for protein stability EMBO reports High 30940648
2014 BRD7 forms a complex with the APC/C (anaphase-promoting complex/cyclosome) and is degraded by APC/C(Cdh1) and APC/C(Cdc20) during the cell cycle; a BRD7 mutant resistant to APC/C-mediated degradation more efficiently suppresses osteosarcoma proliferation and tumor growth. Co-immunoprecipitation, protein stability assay, APC/C inhibitor (proTAME), APC/C degradation-resistant mutant, in vitro and in vivo tumor growth assays Oncotarget Medium 24840027
2020 BRD7 expression is increased by inhibition of the ubiquitin-proteasome pathway upon ferroptosis induction; elevated BRD7 promotes p53 mitochondrial translocation via direct binding to the p53 N-terminal transactivation domain (TAD); site-directed mutation of p53 serine 392 blocks BRD7-p53 binding and prevents p53 mitochondrial translocation and ferroptosis; mitochondrial p53 then interacts with SLC25A28 to enhance its activity, leading to iron accumulation and ferroptosis. CRISPR/Cas9 knockout, BRD7 overexpression plasmid, co-immunoprecipitation, site-directed mutagenesis, subcellular fractionation, SLC25A28 knockdown Redox biology High 32863216
2020 BRD7 is recruited to DNA double-strand break (DSB) sites via ATM-mediated phosphorylation; at DSBs, BRD7 joins PRC2 and NuRD complexes and recruits E3 ubiquitin ligase RNF168, leading to transcriptional repression at active transcription sites flanking DSBs and facilitating DNA repair. Co-immunoprecipitation, ChIP, immunofluorescence, ATM inhibitor treatment, phosphorylation site mapping, RNF168 recruitment assay, DNA repair assays Advanced science High 33101843
2020 BRD7 interacts with YB1 via its N-terminus binding to the C-terminus of YB1; BRD7 decreases YB1 expression by negatively regulating YB1 phosphorylation at Ser102, promoting its proteasomal degradation, thereby suppressing YB1-induced epithelial-mesenchymal transition (EMT), invasion, and metastasis in breast cancer. Co-immunoprecipitation with mass spectrometry, deletion mutant mapping, phosphorylation assay, ubiquitination/proteasome assay, in vitro migration/invasion assays, xenograft and metastatic mouse models Journal of experimental & clinical cancer research High 32028981
2008 BRD7 interacts with BRD2 (co-immunoprecipitation confirmed in mammalian cells); the region from amino acids 430–798 of BRD2 is critical for this interaction; BRD2 localizes in the nucleus in diffuse and dotted patterns. Co-immunoprecipitation, GFP fluorescence, deletion mutant analysis Molecular and cellular biochemistry Low 16786191
2008 The centrosome-associated protein Ceap-16/BLOS2 directly interacts with BRD7 via BRD7's C-terminus and Ceap-16's central region; this interaction translocates Ceap-16 from cytoplasm to nucleus, where it selectively inhibits BRD7's transcriptional suppression of target genes (E2F3, cyclin A); a triple complex of Ceap-16, BRD7, and histones H3/H4 was demonstrated. Co-immunoprecipitation (in vitro and in vivo), domain mapping, reporter assays, localization studies Cellular signalling Medium 18329849
2016 BRD7 knockout mice display an anti-inflammatory phenotype deficiency: BRD7-deficient MEF cells show increased NF-κB (p65) cytoplasm-to-nucleus translocation and elevated inflammatory cytokine expression (IL-6, TNF-α, CXCL-1, iNOS) after LPS stimulation; in vivo, BRD7 KO mice show increased susceptibility to DSS-induced colitis with higher NF-κB nuclear translocation, indicating BRD7 inhibits NF-κB activation during early acute inflammation. BRD7 knockout mouse model, MEF cells, LPS stimulation, NF-κB reporter assay, subcellular fractionation, DSS-induced colitis model Cellular & molecular immunology Medium 27374794
2016 Homozygous BRD7 knockout results in complete male infertility with spermatogenesis arrest at step 13, deformed acrosomal formation, degenerative elongating spermatids, increased apoptosis (TUNEL, annexin V, p53), and elevated DNA damage marker γH2AX; BRD7 is highly expressed in testis and localized to germ cells from pachytene to round spermatid stage. BRD7 knockout mouse model, histology, TUNEL assay, western blot for apoptosis/DNA damage markers, immunohistochemistry Scientific reports Medium 26878912
2015 BRD7 knockout impairs cognitive behavior (but not emotional behavior) and reduces synaptic plasticity-related protein expression, neuronal spine density, and dendritic branching specifically in the medial prefrontal cortex (not hippocampus). BRD7 knockout mouse model, behavioral battery tests, western blot for synaptic proteins, neuronal morphology analysis Behavioural brain research Medium 25721744
2016 BRD7 deficiency leads to embryonic lethality in homozygous knockout mice at mid-gestation (prior to E16.5), establishing an essential developmental role; heterozygous knockdown causes mild changes in glucose metabolism. Homozygous and heterozygous BRD7 knockout mouse models, embryo developmental staging, glucose metabolism assays Journal of cellular and molecular medicine Medium 27444544
2017 BRD7 increases phosphorylation of GSK3β at Ser9 in response to insulin, with this effect occurring even in the absence of AKT activity; BRD7 also mediates phosphorylation of S6K and downstream 4E-BP1; liver-specific BRD7 knockout mice show impaired mTORC1 activity on its downstream targets. BRD7 overexpression, AKT inhibitor, liver-specific BRD7 KO mice, phosphorylation assays (western blot), in vitro and in vivo models Cellular and molecular life sciences Medium 29127434
2018 BRD7 directly interacts with HIF1α and facilitates its degradation via the ubiquitin-proteasome pathway; this reduces LDHA expression and aerobic glycolysis (Warburg effect) in breast cancer cells; bromodomain of BRD7 is required for this activity. Co-immunoprecipitation, ubiquitination assay, bromodomain deletion mutant, LDHA rescue experiment, glycolysis assays, in vivo tumor models Cell death & disease Medium 29725006
2024 TRIM28 acts as an E3 ubiquitin ligase for BRD7; the Coiled-Coil region of TRIM28 binds the N-terminal region of BRD7, mediating K48-linked ubiquitination of BRD7 at K21, leading to proteasomal degradation of BRD7 and promoting breast cancer progression. Co-immunoprecipitation with mass spectrometry, domain mapping, ubiquitination assay (K48 linkage), site-directed mutagenesis (K21), protein stability assay, in vitro and in vivo cancer models Cellular oncology High 39222175
2025 TRIM25 acts as an E3 ubiquitin ligase for BRD7; the PRYSPRY region of TRIM25 binds the N-terminal region of BRD7, increasing K48-linked ubiquitination at K119 and promoting BRD7 proteasomal degradation; this leads to activation of YB1/Bcl-2 signaling, promoting breast cancer malignant progression and paclitaxel resistance. Co-immunoprecipitation, ubiquitination assay (K48-linked, K119 site), domain mapping, protein stability assay, rescue experiment with BRD7 restoration, in vitro and in vivo models Cell death & disease Medium 41315221
2022 BRD7 stabilizes p53 protein by inhibiting AKT-dependent phosphorylation of MDM2 (via the BRD7 bromodomain), thereby reducing phospho-MDM2 binding to p53 and decreasing p53 ubiquitination; this mechanism requires the BRD7 bromodomain. Co-immunoprecipitation, phosphorylation assay, ubiquitination assay, bromodomain deletion mutant, AKT inhibition, p53 knockdown rescue, in vivo xenograft Journal of Cancer Medium 35371302
2021 In colorectal cancer, BRD7 interacts with c-Myc and stabilizes c-Myc protein by inhibiting its ubiquitin-proteasome-dependent degradation; restoration of c-Myc in BRD7-silenced CRC cells rescues cell proliferation and tumor growth. Co-immunoprecipitation, ubiquitination assay, c-Myc rescue experiment, in vitro and in vivo tumor models, AOM/DSS mouse model Frontiers in cell and developmental biology Medium 34109174
2023 BRD7 negatively regulates BIRC2 expression by targeting and inhibiting the activity of the B7BS enhancer region of BIRC2; restoration of BIRC2 rescues the tumor suppressive effects of BRD7 on NPC cell proliferation, migration, invasion, and xenograft tumor growth. ChIP, luciferase enhancer reporter assay, BIRC2 rescue experiment, in vitro and in vivo tumor models Cell death & disease Medium 36788209
2024 BRD7 bridges BRG1 and PBRM1 to the core module of the PBAF complex during CD8+ T cell activation, facilitating PBAF assembly over BAF; the resulting PBAF complex increases chromatin accessibility at the Tbx21 locus and upregulates T-bet expression, driving maturation of short-lived effector CD8+ T cells. BRD7 conditional knockout in CD8+ T cells, co-immunoprecipitation, ATAC-seq (chromatin accessibility), flow cytometry, viral infection models (influenza, LCMV) JCI insight High 38954484
2023 BRD7 competitively inhibits binding of the deubiquitinase USP5 to METTL3, reducing METTL3 protein stability via the ubiquitin-proteasome pathway; this suppresses BRCA1- and RAD51-mediated homologous recombination repair, increasing radiosensitivity of NPC cells. Co-immunoprecipitation, competitive binding assay (BRD7 vs USP5 for METTL3), ubiquitination assay, DNA damage (DSB) assays, homologous recombination repair assays International journal of biological sciences Medium 39664566
2024 BRD7 directly binds EZH2 and recruits DNMT3a to the Clusterin promoter, modulating Clusterin promoter hypermethylation; reduced Clusterin expression suppresses AMPK signaling to facilitate ferroptosis in diabetic testicular damage. Co-immunoprecipitation (BRD7-EZH2, BRD7-DNMT3a), methylation-specific PCR, ChIP assay, BRD7 knockdown, Clusterin rescue experiment Molecular medicine Medium 38992588
2023 BRD7 silencing stabilizes CHK1 (but not CHK2) by increasing USP1 levels and promoting the CHK1-USP1 interaction, enhancing CHK1 deubiquitination; USP1 knockdown abrogates BRD7 silencing-induced CHK1 induction, establishing BRD7 as a negative regulator of CHK1 through the USP1 deubiquitinase axis. BRD7 siRNA, CHK1/CHK2 western blot, co-immunoprecipitation (CHK1-USP1), ubiquitination assay, USP1 knockdown rescue, apoptosis assay Cell death discovery Medium 37626049
2011 miR-200c directly inhibits BRD7 expression; BRD7 loss induced by miR-200c leads to β-catenin nuclear translocation and increased expression of cyclin D1 and c-Myc in endometrial carcinoma cells. miRNA transfection, mRNA microarray, western blot, β-catenin localization assay Gynecologic oncology Low 22015043
2016 BRD7 acts as a transcription factor to up-regulate the expression of miR-3148 at the transcriptional level; miR-3148 then targets the 3'UTR of Wnt3a mRNA to suppress Wnt3a/β-catenin signaling in cervical cancer cells. BRD7 overexpression, luciferase reporter assay for miR-3148 promoter activity, 3'UTR luciferase assay for Wnt3a targeting, western blot Reproductive sciences Low 32046432
2023 BRD7 interacts with InsR (insulin receptor) as shown by co-immunoprecipitation; BRD7 improves glucose homeostasis in liver-specific IRS1/2 double knockout mice, demonstrating that BRD7's effect on insulin signaling operates through an IRS-independent alternative pathway downstream of InsR. Co-immunoprecipitation (BRD7-InsR), liver-specific InsR KO and IRS1/2 KO mouse models, glucose tolerance and insulin signaling assays The Journal of endocrinology Medium 37578842
2025 Brd7 loss in breast cancer metastatic dormancy models induces metastatic reawakening; Brd7-deficient cells downregulate MHC-1 expression and promote a pro-metastatic cytokine profile, increasing pro-tumorigenic neutrophils, CD8+ exhausted T cells, and CD4+ stress-response T cells in the lung microenvironment; neutrophil depletion, NET inhibition, or immune checkpoint therapy abrogates this metastatic outgrowth. In vivo PBAF subunit screen, flow cytometry, single-cell RNA-seq, epigenomic profiling, neutrophil depletion, NET inhibition, immune checkpoint therapy Nature communications High 39910049

Source papers

Stage 0 corpus · 97 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2018 Iterative Design and Optimization of Initially Inactive Proteolysis Targeting Chimeras (PROTACs) Identify VZ185 as a Potent, Fast, and Selective von Hippel-Lindau (VHL) Based Dual Degrader Probe of BRD9 and BRD7. Journal of medicinal chemistry 252 30540463
2008 BRD7, a novel PBAF-specific SWI/SNF subunit, is required for target gene activation and repression in embryonic stem cells. The Journal of biological chemistry 193 18809673
2020 The BRD7-P53-SLC25A28 axis regulates ferroptosis in hepatic stellate cells. Redox biology 167 32863216
2010 BRD7 is a candidate tumour suppressor gene required for p53 function. Nature cell biology 167 20228809
2010 Polybromo-associated BRG1-associated factor components BRD7 and BAF180 are critical regulators of p53 required for induction of replicative senescence. Proceedings of the National Academy of Sciences of the United States of America 147 20660729
2010 BRD7, a subunit of SWI/SNF complexes, binds directly to BRCA1 and regulates BRCA1-dependent transcription. Cancer research 108 20215511
2009 TRIM24 mediates ligand-dependent activation of androgen receptor and is repressed by a bromodomain-containing protein, BRD7, in prostate cancer cells. Biochimica et biophysica acta 86 19909775
2014 BRD7, a tumor suppressor, interacts with p85α and regulates PI3K activity. Molecular cell 76 24657164
2004 BRD7, a novel bromodomain gene, inhibits G1-S progression by transcriptionally regulating some important molecules involved in ras/MEK/ERK and Rb/E2F pathways. Journal of cellular physiology 75 15137061
2007 BRD7 suppresses the growth of Nasopharyngeal Carcinoma cells (HNE1) through negatively regulating beta-catenin and ERK pathways. Molecular and cellular biochemistry 70 17458518
2014 BRD7 regulates XBP1s' activity and glucose homeostasis through its interaction with the regulatory subunits of PI3K. Cell metabolism 62 24836559
2016 BRD7: a novel tumor suppressor gene in different cancers. American journal of translational research 58 27158366
2007 Solution structure of BRD7 bromodomain and its interaction with acetylated peptides from histone H3 and H4. Biochemical and biophysical research communications 55 17498659
2006 The transcriptional regulation role of BRD7 by binding to acetylated histone through bromodomain. Journal of cellular biochemistry 55 16265664
2016 miR-141 is involved in BRD7-mediated cell proliferation and tumor formation through suppression of the PTEN/AKT pathway in nasopharyngeal carcinoma. Cell death & disease 54 27010857
2014 Disturbing miR-182 and -381 inhibits BRD7 transcription and glioma growth by directly targeting LRRC4. PloS one 54 24404152
2011 The interactions between MicroRNA-200c and BRD7 in endometrial carcinoma. Gynecologic oncology 53 22015043
2015 Up-Regulation of MiR-300 Promotes Proliferation and Invasion of Osteosarcoma by Targeting BRD7. PloS one 52 26010572
2013 Tumor suppressive effects of bromodomain-containing protein 7 (BRD7) in epithelial ovarian carcinoma. Clinical cancer research : an official journal of the American Association for Cancer Research 51 24198243
2019 Poly(ADP-ribosyl)ation of BRD7 by PARP1 confers resistance to DNA-damaging chemotherapeutic agents. EMBO reports 49 30940648
2016 Knockout of BRD7 results in impaired spermatogenesis and male infertility. Scientific reports 48 26878912
2003 Regulation of transcription by the heterogeneous nuclear ribonucleoprotein E1B-AP5 is mediated by complex formation with the novel bromodomain-containing protein BRD7. The Biochemical journal 47 12489984
2016 BRD7 plays an anti-inflammatory role during early acute inflammation by inhibiting activation of the NF-кB signaling pathway. Cellular & molecular immunology 46 27374794
2006 Identification of nuclear localization signal that governs nuclear import of BRD7 and its essential roles in inhibiting cell cycle progression. Journal of cellular biochemistry 42 16475162
2012 Mutation analysis of BRCA1, BRCA2, PALB2 and BRD7 in a hospital-based series of German patients with triple-negative breast cancer. PloS one 41 23110154
2020 BRD7 suppresses invasion and metastasis in breast cancer by negatively regulating YB1-induced epithelial-mesenchymal transition. Journal of experimental & clinical cancer research : CR 39 32028981
2020 ATM-Dependent Recruitment of BRD7 is required for Transcriptional Repression and DNA Repair at DNA Breaks Flanking Transcriptional Active Regions. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 38 33101843
2018 BRD7 expression and c-Myc activation forms a double-negative feedback loop that controls the cell proliferation and tumor growth of nasopharyngeal carcinoma by targeting oncogenic miR-141. Journal of experimental & clinical cancer research : CR 38 29559001
2008 Promoter methylation inhibits BRD7 expression in human nasopharyngeal carcinoma cells. BMC cancer 37 18778484
1999 Identification and molecular characterization of BP75, a novel bromodomain-containing protein. FEBS letters 35 10526152
2016 BRD7 mediates hyperglycaemia-induced myocardial apoptosis via endoplasmic reticulum stress signalling pathway. Journal of cellular and molecular medicine 34 27957794
2000 Molecular characterization of celtix-1, a bromodomain protein interacting with the transcription factor interferon regulatory factor 2. Journal of cellular physiology 34 11025449
2008 Transcriptional regulation of BRD7 expression by Sp1 and c-Myc. BMC molecular biology 33 19111069
2020 Application of Atypical Acetyl-lysine Methyl Mimetics in the Development of Selective Inhibitors of the Bromodomain-Containing Protein 7 (BRD7)/Bromodomain-Containing Protein 9 (BRD9) Bromodomains. Journal of medicinal chemistry 32 32410449
2015 MicroRNA-410 promotes cell proliferation by targeting BRD7 in non-small cell lung cancer. FEBS letters 32 26149213
2014 Targeting the anaphase-promoting complex/cyclosome (APC/C)- bromodomain containing 7 (BRD7) pathway for human osteosarcoma. Oncotarget 32 24840027
2020 Structural Basis of Inhibitor Selectivity in the BRD7/9 Subfamily of Bromodomains. Journal of medicinal chemistry 30 32091206
2010 Gene regulation and tumor suppression by the bromodomain-containing protein BRD7. Cell cycle (Georgetown, Tex.) 28 20647772
2003 Expression of tumor related genes NGX6, NAG-7, BRD7 in gastric and colorectal cancer. World journal of gastroenterology 28 12918109
2016 Bromodomain-containing protein 7 (BRD7) as a potential tumor suppressor in hepatocellular carcinoma. Oncotarget 26 26919247
2018 BRD7 inhibits the Warburg effect and tumor progression through inactivation of HIF1α/LDHA axis in breast cancer. Cell death & disease 25 29725006
2015 Inactivation of BRD7 results in impaired cognitive behavior and reduced synaptic plasticity of the medial prefrontal cortex. Behavioural brain research 25 25721744
2021 BRD7 Promotes Cell Proliferation and Tumor Growth Through Stabilization of c-Myc in Colorectal Cancer. Frontiers in cell and developmental biology 24 34109174
2023 BRD7 inhibits enhancer activity and expression of BIRC2 to suppress tumor growth and metastasis in nasopharyngeal carcinoma. Cell death & disease 23 36788209
2020 Emerging Roles of BRD7 in Pathophysiology. International journal of molecular sciences 23 32992509
2016 XIAP-associating factor 1, a transcriptional target of BRD7, contributes to endothelial cell senescence. Oncotarget 23 26802028
2003 BP75, bromodomain-containing M(r) 75,000 protein, binds dishevelled-1 and enhances Wnt signaling by inactivating glycogen synthase kinase-3 beta. Cancer research 20 12941796
2020 4-Acyl Pyrroles as Dual BET-BRD7/9 Bromodomain Inhibitors Address BETi Insensitive Human Cancer Cell Lines. Journal of medicinal chemistry 18 33275431
2006 BRD2 is one of BRD7-interacting proteins and its over-expression could initiate apoptosis. Molecular and cellular biochemistry 18 16786191
2017 A genomic screen for angiosuppressor genes in the tumor endothelium identifies a multifaceted angiostatic role for bromodomain containing 7 (BRD7). Angiogenesis 16 28951988
2016 NMR Fragment Screening Hit Induces Plasticity of BRD7/9 Bromodomains. Chembiochem : a European journal of chemical biology 16 27194508
2016 The role of BRD7 in embryo development and glucose metabolism. Journal of cellular and molecular medicine 16 27444544
2016 BRD7 Acts as a Tumor Suppressor Gene in Lung Adenocarcinoma. PloS one 16 27580131
2017 BRD7 regulates the insulin-signaling pathway by increasing phosphorylation of GSK3β. Cellular and molecular life sciences : CMLS 15 29127434
2024 TRIM28 promotes tumor growth and metastasis in breast cancer by targeting the BRD7 protein for ubiquitination and degradation. Cellular oncology (Dordrecht, Netherlands) 14 39222175
2023 Epstein-Barr Virus Synergizes with BRD7 to Conquer c-Myc-Mediated Viral Latency Maintenance via Chromatin Remodeling. Microbiology spectrum 13 36728436
2021 BRD7 inhibits tumor progression by positively regulating the p53 pathway in hepatocellular carcinoma. Journal of Cancer 13 33531996
2008 Ceap/BLOS2 interacts with BRD7 and selectively inhibits its transcription-suppressing effect on cellular proliferation-associated genes. Cellular signalling 13 18329849
2024 BRD7 enhances the radiosensitivity of nasopharyngeal carcinoma cells by negatively regulating USP5/METTL3 axis-mediated homologous recombination repair. International journal of biological sciences 12 39664566
2022 LncRNA LENGA acts as a tumor suppressor in gastric cancer through BRD7/TP53 signaling. Cellular and molecular life sciences : CMLS 12 36477655
2016 Development of chemical probes for the bromodomains of BRD7 and BRD9. Drug discovery today. Technologies 12 27769361
2025 Brd7 loss reawakens dormant metastasis initiating cells in lung by forging an immunosuppressive niche. Nature communications 11 39910049
2022 BRD7 Stabilizes P53 via Dephosphorylation of MDM2 to Inhibit Tumor Growth in Breast Cancer Harboring Wild-type P53. Journal of Cancer 11 35371302
2020 BRD7-Mediated miR-3148 Inhibits Progression of Cervical Cancer by Targeting Wnt3a/β-Catenin Pathway. Reproductive sciences (Thousand Oaks, Calif.) 11 32046432
2023 Rational Design and Development of Selective BRD7 Bromodomain Inhibitors and Their Activity in Prostate Cancer. Journal of medicinal chemistry 10 37552884
2019 BRD7 deficiency leads to the development of obesity and hyperglycemia. Scientific reports 10 30926848
2021 Bromodomain-containing protein 7 regulates matrix metabolism and apoptosis in human nucleus pulposus cells through the BRD7-PI3K-YAP1 signaling axis. Experimental cell research 9 34038745
2025 BRD7 Inhibited Immune Escape in Nasopharyngeal Carcinoma via Inhibiting PD-L1 Expression. International journal of biological sciences 8 40083706
2024 BRD7 facilitates ferroptosis via modulating clusterin promoter hypermethylation and suppressing AMPK signaling in diabetes-induced testicular damage. Molecular medicine (Cambridge, Mass.) 8 38992588
2012 No evidence for breast cancer susceptibility associated with variants of BRD7, a component of p53 and BRCA1 pathways. Familial cancer 8 22864638
2007 Preparation of polyclonal antibody specific for BRD7 and detection of its expression pattern in the human fetus. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 8 18071067
2006 Cloning and characterization of the BRD7 gene promoter. DNA and cell biology 8 16792505
2003 [Expression of BRD7-interacting proteins,BRD2 and BRD3, in nasopharyngeal carcinoma tissues]. Ai zheng = Aizheng = Chinese journal of cancer 8 12600283
2023 AGAP2-AS1/BRD7/c-Myc signaling axis promotes skin cutaneous melanoma progression. American journal of translational research 7 36777828
2022 The regulatory subunits of PI3K, p85α and p85β, differentially affect BRD7-mediated regulation of insulin signaling. Journal of molecular cell biology 7 34751372
2022 BRD7 restrains TNF-α-induced proliferation and migration of airway smooth muscle cells by inhibiting notch signaling. Experimental lung research 7 35943053
2015 BRD7 promoter hypermethylation as an indicator of well differentiated oral squamous cell carcinomas. Asian Pacific journal of cancer prevention : APJCP 7 25743841
2024 BRD7 regulates cellular senescence and apoptosis in ALS by modulating p21 expression and p53 mitochondrial translocation respectively. Neuroscience 6 39510439
2003 [Researching a novel NPC-related candidate suppressor gene BRD7 by two-dimensional gel electrophoresis and MALDI-TOF-MS]. Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica 6 12958654
2024 BRD7 as key factor in PBAF complex assembly and CD8+ T cell differentiation. JCI insight 5 38954484
2022 Upregulation of BRD7 protects podocytes against high glucose-induced apoptosis by enhancing Nrf2 in a GSK-3β-dependent manner. Tissue & cell 5 35550209
2002 Screening of BRD7 interacting proteins by yeast two-hybrid system. Science in China. Series C, Life sciences 5 18759043
2021 Is BRD7 associated with spermatogenesis impairment and male infertility in humans? A case-control study in a Han Chinese population. Basic and clinical andrology 4 34470615
2023 BRD7 suppresses tumor chemosensitivity to CHK1 inhibitors by inhibiting USP1-mediated deubiquitination of CHK1. Cell death discovery 3 37626049
2002 Analysis of bromodomain of BRD-7 gene and its prokaryotic expression. Ai zheng = Aizheng = Chinese journal of cancer 3 12526210
2024 Tumor- and host-derived heparanase-2 (Hpa2) attenuates tumorigenicity: role of Hpa2 in macrophage polarization and BRD7 nuclear localization. Cell death & disease 2 39695102
2023 Identification of BRD7 by whole-exome sequencing as a predictor for intermediate-stage hepatocellular carcinoma in patients undergoing TACE. Journal of cancer research and clinical oncology 2 37365429
2023 BRD7 improves glucose homeostasis independent of IRS proteins. The Journal of endocrinology 2 37578842
2019 Nuclear trafficking dynamics of Bromodomain-containing protein 7 (BRD7), a switch/sucrose non-fermentable (SWI/SNF) chromatin remodelling complex subunit, in porcine oocytes and cleavage-stage embryos. Reproduction, fertility, and development 2 31079594
2011 [Definition and function identification of nucleus export signal of BRD7]. Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 2 21873788
2026 Targeted demethylation of the BRD7 promoter based on CRISPR/dCas9 system inhibits the malignant progression of nasopharyngeal carcinoma. Clinical and translational medicine 1 41510594
2025 TRIM25 degrades BRD7 protein stability through the ubiquitin proteasome pathway to promote breast cancer progression and paclitaxel resistance by activating YB1/Bcl-2 transcription axis. Cell death & disease 1 41315221
2022 Depletion of SMARCB1 and BRD7, two SWI/SNF chromatin remodelling complex subunits, differentially impact porcine embryo development. Reproduction, fertility, and development 1 35296373
2022 Transforming Stimulated Clone 22 (TSC-22) Interacts Directly with Bromodomain-Containing Protein 7 (BRD7) to Enhance the Inhibition of Extracellular Signal-Regulate Kinase (ERK) Pathway in Ovarian Cancer. Development & reproduction 1 36285148
2008 [Expression and SNP analysis of BRD7 gene in acute leukemia cells]. Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 1 18772500
2025 Targeted suppression of BRD7 with BI-9564 prevents seizure behaviors in pentylenetetrazol and pilocarpine-induced mouse model of epilepsy. Behavioural brain research 0 40120945
2016 [Construction of bromodomain-deleted BRD7 mutation vector based on homologous recombination and reverse PCR amplification]. Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 0 27640804

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