Affinage

ARID4A

AT-rich interactive domain-containing protein 4A · UniProt P29374

Round 2 corrected
Length
1257 aa
Mass
142.8 kDa
Annotated
2026-04-28
86 papers in source corpus 22 papers cited in narrative 21 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ARID4A is a nuclear transcriptional corepressor that bridges the retinoblastoma protein (pRb) to the mSIN3–SAP30–HDAC chromatin-remodeling complex, thereby repressing E2F-dependent transcription and enforcing G1 cell-cycle arrest and cellular senescence (PMID:1857421, PMID:9651585, PMID:11283269, PMID:16479010). It harbors two repression domains—R1, which operates through SUMOylation-dependent mechanisms, and R2, which recruits class I HDACs via SAP30—and CDK2-mediated phosphorylation at S864/S1007 dissociates ARID4A from pRb at the G1–S transition, relieving repression (PMID:16479010, PMID:21148318). Beyond transcriptional repression, ARID4A regulates genomic imprinting at the PWS/AS locus through modulation of histone methylation and DNA methylation, acts as a leukemia and metastasis suppressor whose loss causes myeloid malignancy in mice, and functions as an RNA-binding protein that stabilizes metastasis-suppressor transcripts via 3′UTR elements (PMID:17043311, PMID:18728284, PMID:40066676). Its tandem PWWP–ARID supramodule binds DNA without sequence preference and coordinately engages modified histones (H3K4me3, H4K20me3), with an internal acidic loop acting as a DNA mimic to regulate chromatin association (PMID:34506790, PMID:35365808).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 1991 High

    Establishing that ARID4A is a direct pRb-binding protein revealed a new class of pocket-protein interactor and raised the question of what effector function ARID4A brings to the Rb pathway.

    Evidence cDNA expression library screen with recombinant pRb, in vitro binding with mutagenesis and E7 competition

    PMID:1857421

    Open questions at the time
    • No indication of ARID4A's transcriptional or enzymatic function
    • Binding site on pRb not precisely mapped
  2. 1998 High

    Identification of ARID4A as a subunit of the mSIN3–SAP30–HDAC complex answered how pRb achieves HDAC-dependent gene silencing—ARID4A acts as the physical bridge recruiting this corepressor complex to the pocket domain.

    Evidence Biochemical purification of the mSIN3A complex from human cells, co-immunoprecipitation, mass spectrometry

    PMID:10321733 PMID:10490602 PMID:9651585

    Open questions at the time
    • Stoichiometry and architecture of the ARID4A–mSIN3–HDAC holocomplex unresolved
    • Relative contribution of R1 vs R2 repression domains to growth suppression not yet dissected
  3. 2001 High

    Demonstrating that the entire ARID4A–mSIN3–HDAC complex colocalizes with pRb/E2F4 at replication origins in quiescent cells placed ARID4A at a defined nuclear site and implied a role in DNA replication licensing control.

    Evidence Co-IP, immunofluorescence colocalization, HDAC activity assays, and cell fractionation in quiescent human fibroblasts

    PMID:11283269

    Open questions at the time
    • Direct role at replication origins not tested by functional assays
    • Whether ARID4A is required for replication origin silencing remains untested
  4. 2003 High

    Identification of BRMS1 and SAP180 (ARID4B) as ARID4A interaction partners expanded the mSIN3 complex membership and connected ARID4A to metastasis suppression and paralog redundancy.

    Evidence Yeast two-hybrid, reciprocal co-IP, gel filtration in breast cancer and melanoma lines; biochemical purification from K562 cells

    PMID:12724404 PMID:14581478

    Open questions at the time
    • Functional distinction between ARID4A and ARID4B within the complex unclear
    • Whether BRMS1–ARID4A interaction is essential for metastasis suppression not resolved until later mutant studies
  5. 2006 High

    Dissection of ARID4A's two repression domains revealed that R1 acts through SUMOylation and R2 through HDAC recruitment, and both are required for growth arrest and senescence—answering how a single protein enforces dual modes of transcriptional silencing.

    Evidence SUMOylation assays, transcriptional repression assays, domain deletions, cell proliferation and senescence marker analysis

    PMID:16479010

    Open questions at the time
    • Identity of SUMO E3 ligase targeting R1 unknown
    • Endogenous genomic targets repressed by R1 vs R2 not determined
  6. 2006 High

    Mouse genetic studies showed ARID4A cooperates with ARID4B and Rb to maintain imprinting at the PWS/AS locus through histone H3K9/H4K20 trimethylation and DNA methylation, extending its role from transcriptional repression to epigenetic imprint maintenance.

    Evidence Gene-trap mutagenesis, compound Arid4a/Arid4b knockout mice, ChIP for histone marks, genetic suppression of AS imprinting defect

    PMID:17043311

    Open questions at the time
    • Which histone methyltransferase is recruited by ARID4A not identified
    • Whether imprinting role is direct or mediated through global Rb pathway not resolved
  7. 2008 High

    Two key regulatory inputs were identified: CDK2 phosphorylation at S864/S1007 dissociates ARID4A from pRb to relieve repression at G1–S, while SIRT1 recruited by ING proteins antagonizes R2-mediated HDAC1 repression, establishing how cell cycle and sirtuin signaling converge on the ARID4A–Rb axis.

    Evidence In vitro kinase assay with mutagenesis, cell-cycle-synchronized co-IP (CDK2); binding assays with sirtuin activator/inhibitor (SIRT1)

    PMID:18193082 PMID:21148318

    Open questions at the time
    • Whether CDK2 phosphorylation occurs in vivo on endogenous ARID4A not confirmed by phospho-specific antibody
    • How SIRT1-mediated deacetylation specifically inactivates R2 mechanistically unclear
  8. 2008 High

    Arid4a knockout mice developed myelodysplastic disorder progressing to AML with altered H3K4/H3K9/H4K20 trimethylation and deregulated Hox gene expression, establishing ARID4A as a bona fide leukemia suppressor.

    Evidence Arid4a-null mice, complete blood counts, flow cytometry, histology, RT-PCR, histone mark western blots

    PMID:18728284

    Open questions at the time
    • Whether leukemia suppression is Rb-dependent or reflects Rb-independent ARID4A functions not determined
    • Direct target genes driving leukemogenesis upon ARID4A loss not comprehensively mapped
  9. 2013 High

    Discovery that ARID4A and ARID4B function as transcriptional coactivators for the androgen receptor in Sertoli cells, with their loss phenocopying AR and Rb knockouts, revealed a tissue-specific coactivator role beyond the canonical corepressor paradigm.

    Evidence Sertoli cell-specific compound knockout mice, co-IP for ARID4A–ARID4B interaction, RT-PCR of AR/Rb target genes, histology and barrier assays

    PMID:23487765

    Open questions at the time
    • Mechanism by which ARID4A switches from corepressor to coactivator not identified
    • Whether direct AR interaction occurs or is mediated by Rb not tested
  10. 2021 High

    Structural determination of the PWWP–ARID supramodule by NMR revealed that an internal acidic loop (L12) acts as a DNA mimic, auto-inhibiting DNA binding and allosterically enhancing chromo barrel recognition of H3K4me3 and H4K20me3, providing a mechanistic basis for coordinated chromatin engagement.

    Evidence NMR structure, NMR titration, ITC, domain deletion analysis

    PMID:34506790

    Open questions at the time
    • Whether L12 autoinhibition is relieved by post-translational modifications in vivo unknown
    • Which genomic loci require loop L12-mediated regulation not mapped
  11. 2022 High

    A schizophrenia-associated missense variant (p.His411Asp) in the PWWP–ARID supramodule was shown to destabilize the domain, reduce DNA binding, and impair G1 arrest, linking ARID4A chromatin engagement to a neuropsychiatric phenotype.

    Evidence Crystal structure at 2.05 Å, DNA-binding assays, cell cycle and proliferation assays on wild-type vs mutant, whole-exome sequencing of schizophrenia cohort

    PMID:35365808

    Open questions at the time
    • Causality of the variant in schizophrenia not established by family segregation or animal model
    • Whether the structural defect affects histone-mark recognition in addition to DNA binding not tested
  12. 2025 Medium

    Demonstration that ARID4A is an RNA-binding protein that stabilizes metastasis-suppressor transcripts (MTSS1, TIMP2, Rb1, PTEN) via conserved 3′UTR elements expanded its functional repertoire beyond chromatin-directed transcriptional regulation to post-transcriptional mRNA stability control.

    Evidence RIP, RIP-ChIP, EMSA, luciferase 3′UTR reporters, mRNA stability assays, in vivo breast cancer metastasis model

    PMID:40066676

    Open questions at the time
    • RNA-binding specificity determinants within the ARID domain not structurally resolved
    • Whether RNA binding is independent of or competitive with DNA/chromatin binding untested
    • Single-lab finding awaiting independent replication

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include: the structural basis for ARID4A's switch between corepressor and coactivator functions, the identity of SUMO E3 ligases and histone methyltransferases it recruits, the full repertoire of RNA targets and whether RNA and DNA binding are mutually exclusive, and whether ARID4A's roles in leukemia suppression and imprinting are Rb-dependent or autonomous.
  • No reconstituted holocomplex structure available
  • Genome-wide direct target map by ChIP-seq in a physiological context not reported
  • In vivo validation of RNA-binding function in development/disease models lacking

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 4 GO:0003677 DNA binding 3 GO:0060090 molecular adaptor activity 3 GO:0003723 RNA binding 1 GO:0042393 histone binding 1
Localization
GO:0005634 nucleus 2 GO:0005654 nucleoplasm 1
Pathway
R-HSA-4839726 Chromatin organization 4 R-HSA-74160 Gene expression (Transcription) 3 R-HSA-1640170 Cell Cycle 2 R-HSA-1643685 Disease 2
Partners
ARID4BBRMS1HDAC1HDAC2RB1SAP30SIN3ASIRT1
Complex memberships
mSIN3A–HDAC corepressor complexpRb–E2F repressor complex

Evidence

Reading pass · 21 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1991 ARID4A (RBP1) was identified as a cellular protein that directly binds to the retinoblastoma gene product pRb through a conserved LXCXE-like motif. In vitro binding assays showed RBP1 specifically binds pRb, and this interaction is disrupted by HPV-16 E7 protein and by mutations in the putative pRb-binding segment of RBP1. cDNA expression library screen with recombinant pRb, in vitro binding assay, site-directed mutagenesis Nature High 1857421
1993 RBP1 (ARID4A) is a large (~200 kDa) nuclear phosphoprotein ubiquitously expressed, and forms stable complexes with pRb in vivo that are dissociated by purified HPV E7 protein. The protein undergoes alternative splicing in an internal exon containing potential casein kinase II and p34cdc2 phosphorylation sites, generating a family of RB-binding isoforms. cDNA cloning, immunoblot, co-immunoprecipitation, in vivo complex analysis, in vitro RB-binding assay Oncogene High 8414517 8455946
1999 RBP1 (ARID4A) functions as a transcriptional corepressor that recruits both HDAC-dependent and HDAC-independent repression activities to the pRb family pocket. RBP1 contains two repression domains: one (R2) binds all three known HDACs (HDAC1, HDAC2, HDAC3) and represses transcription in an HDAC-dependent manner; the other functions independently of HDACs. Overexpression of RBP1 inhibits E2F-mediated transcription and suppresses cell growth. Transcriptional repression assays, co-immunoprecipitation, HDAC activity assays, cell proliferation assays Molecular and cellular biology High 10321733 10490602
1998 RBP1 (ARID4A) is a component of the mSIN3-SAP30-HDAC complex. SAP30 was identified as a component of a human histone deacetylase complex that includes Sin3, HDAC1/2, and RBP1 as a bridging factor connecting pRb to this repressor complex. Biochemical purification, co-immunoprecipitation, mass spectrometry identification of complex components Molecular cell High 9651585
2001 RBP1 (ARID4A) recruits the mSIN3-SAP30-HDAC complex (containing exclusively class I HDACs) to the pRb family pocket via the LXCXE motif. In quiescent normal human cells, the entire RBP1-mSIN3-SAP30-HDAC complex colocalizes with RB family members and E2F4 in discrete nuclear regions corresponding to initial origins of DNA replication. RBP1 accounts for the majority of RB-associated HDAC activity. Co-immunoprecipitation, immunofluorescence colocalization, HDAC activity assays, cell fractionation Molecular and cellular biology High 11283269
2003 BRMS1 (breast cancer metastasis suppressor 1) interacts with ARID4A (RBP1) and at least seven members of the mSIN3 HDAC complex in human breast and melanoma cell lines. BRMS1 co-immunoprecipitates enzymatically active HDAC proteins and exists in large mSIN3 complexes (~1.4–1.9 MDa). ARID4A is thus a component of a SIN3-HDAC chromatin remodeling complex involved in metastasis suppression. Yeast two-hybrid, co-immunoprecipitation, gel filtration, transcriptional repression assay The Journal of biological chemistry High 14581478
2003 SAP180, a protein 40% identical to RBP1/ARID4A, was identified as a novel mSIN3A-associated protein. RBP1 itself is confirmed as a component of the mSIN3A corepressor complex; all SAPs (including RBP1) bind to the HDAC-interaction domain (HID) of mSIN3A, suggesting that the HID functions as an assembly interface for the mSIN3A corepressor complex. Biochemical purification from K562 cells, co-immunoprecipitation, gel filtration, transcriptional repression assay Molecular and cellular biology High 12724404
2006 RBP1 (ARID4A) family members exhibit SUMOylation-dependent transcriptional repression. The R1 repression region contains a SUMOylated moiety (R1σ) that represses both basal and activated transcription in a SUMOylation-dependent manner, and an alpha-helical region (R1α) embedded within the ARID domain that represses only basal transcription. Overexpression of RBP1 causes profound inhibition of cell proliferation and induces expression of a senescence marker, requiring both R1 and R2 domains. Transcriptional repression assays, SUMOylation analysis, cell proliferation/senescence assays, domain deletion analysis Molecular and cellular biology High 16479010
2006 Rbbp1/Arid4a and Rbbp1l1/Arid4b regulate genomic imprinting at the PWS/AS domain. RBBP1/ARID4A physically interacts with RBBP1L1/ARID4B and with the Snrpn promoter. Combined homozygous deficiency for Arid4a and heterozygous deficiency for Arid4b altered epigenetic modifications at the PWS-IC, reducing trimethylation of histone H4K20 and H3K9 and reducing DNA methylation. Mutations in Arid4a, Arid4b, or Rb suppressed an AS imprinting defect. Gene trap mutagenesis, mouse knockout, chromatin immunoprecipitation, western blot, immunofluorescence, genetic epistasis Genes & development High 17043311
2008 SIRT1 negatively regulates HDAC1-dependent transcriptional repression by RBP1 (ARID4A). The HDAC-dependent R2 domain of RBP1 is necessary and sufficient for interaction with SIRT1. The ING1b and ING2 subunits of the mSIN3A/HDAC1 complex recruit SIRT1 to the R2 domain. SIRT1 activity inhibits R2-mediated mSIN3A/HDAC1 transcriptional repression, establishing a class III HDAC regulatory mechanism over class I HDAC activity. In vitro and in vivo binding assays, transcriptional repression assays with sirtuin activator (resveratrol) and inhibitor (sirtinol), domain mapping Oncogene High 18193082
2008 Arid4a functions as a leukemia suppressor gene. Arid4a-deficient mice develop ineffective hematopoiesis, transition to CMML-like myelodysplastic/myeloproliferative disorder, and then AML. Arid4a deficiency causes increased trimethylation of H3K4, H3K9, and H4K20 in bone marrow. Hox gene expression (Hoxb3, Hoxb5, Hoxb6, Hoxb8) and FoxP3 are reduced in Arid4a-deficient bone marrow. Mouse knockout, complete blood counts, flow cytometry, histology, RT-PCR, western blot, immunofluorescence Journal of the National Cancer Institute High 18728284
2008 BRMS1 interaction with ARID4A is required for specific aspects of SIN3-HDAC complex function. BRMS1(L174D) disrupts direct ARID4A interaction in yeast two-hybrid but retains indirect association by co-IP; BRMS1(ΔCC1) prevents co-IP association. These mutations alter complex composition, impair basal transcription repression, and differentially regulate osteopontin, but both mutants still suppress metastasis by downregulating EGFR. Yeast two-hybrid, co-immunoprecipitation, gene expression analysis, xenograft metastasis assay The Journal of biological chemistry Medium 18211900
2010 CDK2 phosphorylates RBP1 (ARID4A) on serines 864 and 1007 (flanking the LXCXE motif). CDK2-mediated phosphorylation of RBP1 or pRb destabilizes their interaction in vitro, and concurrent phosphorylation of both proteins causes their dissociation, releasing the mSIN3-HDAC transcriptional repressor complex from pRb. RBP1 phosphorylation increases during G1-to-S phase progression in MCF-7 cells. In vitro kinase assay, in vitro binding assay, cell cycle synchronization, co-immunoprecipitation, mutagenesis The Journal of biological chemistry High 21148318
2013 ARID4A and ARID4B physically interact with each other and are required for male fertility. In Sertoli cells, ARID4A and ARID4B function as transcriptional coactivators for androgen receptor (AR) and RB, regulating AR- and RB-responsive genes. Their combined deficiency causes spermatogenic arrest, impaired blood-testis barrier integrity, hypogonadism, and seminal vesicle agenesis, phenocopying Sertoli cell-specific AR and RB knockouts. Mouse knockout, co-immunoprecipitation (ARID4A-ARID4B interaction), RT-PCR analysis of downstream targets, histology, barrier permeability assays Proceedings of the National Academy of Sciences of the United States of America High 23487765
2018 ARID4A and ARID4B are direct target genes of microRNA-30d in prostate cancer. Knockout of ARID4A and ARID4B promotes PCa cell proliferation, migration, and invasion in vitro, indicating a tumor suppressor function. Luciferase reporter assay (miR-30d targeting), CRISPR/siRNA knockdown, cell proliferation/migration/invasion assays Journal of cellular biochemistry Medium 29797600
2018 The chromo barrel domain of RBBP1 (ARID4A) was crystallized. Structural analysis indicates it could interact with histone H4K20me3 similarly to other chromo barrel domains, but ITC assays showed DNA does not significantly enhance H4K20me3 binding by the isolated chromo barrel domain, and the domain lacks a typical DNA-binding surface. X-ray crystallography, isothermal titration calorimetry (ITC) Biochemical and biophysical research communications Medium 29408527
2021 The tandem PWWP-ARID domain structure of RBBP1 (ARID4A) was determined by NMR. The ARID domain binds DNA without AT-rich or GC-rich sequence preference. A long flexible acidic loop L12 within the ARID domain acts as a DNA mimic, binding to the ARID DNA-binding region and inhibiting DNA binding in cis. Loop L12 also binds weakly to Tudor and chromo barrel domains of RBBP1, and more strongly to histone H2A-H2B. Both L12 and DNA enhance the chromo barrel domain's binding to H3K4me3 and H4K20me3, supporting a model of coordinated chromatin recognition. NMR structure determination, NMR titration, isothermal titration calorimetry, domain deletion analysis Journal of molecular biology High 34506790
2022 A missense variant ARID4A p.His411Asp was identified in schizophrenia patients. The crystal structure of the PWWP-ARID tandem domain at 2.05 Å resolution revealed that PWWP and ARID form a structural supramodule. The p.His411Asp mutant adopts a less compact conformation, exhibits weaker dsDNA-binding, decreases G0-G1 cell cycle arrest, and promotes HEK293T cell proliferation, linking ARID4A's DNA-binding function to G1 cell cycle arrest. Whole-exome sequencing, X-ray crystallography (2.05 Å), DNA-binding assays, cell cycle analysis, cell proliferation assay, mutagenesis Molecular psychiatry High 35365808
2025 ARID4A (Arid4a) functions as an RNA-binding protein that stabilizes transcripts of metastasis-suppressing genes including MTSS1, TIMP2, Rb1, and PTEN by binding to a conserved structural RNA element in their 3'UTRs. The ARID domain of Arid4a is required for this mRNA stabilization activity. Loss of Arid4a promotes breast tumor metastasis in vitro and in vivo. RNA immunoprecipitation (RIP), RIP-ChIP, EMSA, luciferase assay, mRNA stability assay, in vitro/in vivo metastasis experiments Cancer medicine Medium 40066676
2025 HERC2 binds to ARID4A through a 'DxDKDxD' motif recognized by the RLD2 domain of HERC2, identifying ARID4A as one of a small set of HERC2-interacting proteins relevant to brain development. Quantitative binding assays, X-ray crystallography, sequence conservation analysis bioRxivpreprint Low
2005 A survey of DNA-binding properties across the ARID family showed that ARID4A's ARID domain, like most ARID subfamily members, binds DNA without obvious sequence preference (not restricted to AT-rich sequences). DNA-binding affinity varies between subfamilies, and site-specific mutagenesis did not support specific amino acid determinants in Loop 2 or Helix 5 as primary drivers of sequence specificity. In vitro DNA-binding assays, site-specific mutagenesis across ARID family Nucleic acids research Medium 15640446

Source papers

Stage 0 corpus · 86 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2004 Large-scale characterization of HeLa cell nuclear phosphoproteins. Proceedings of the National Academy of Sciences of the United States of America 1159 15302935
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
1998 A novel human WD protein, h-beta TrCp, that interacts with HIV-1 Vpu connects CD4 to the ER degradation pathway through an F-box motif. Molecular cell 575 9660940
2006 ING tumor suppressor proteins are critical regulators of chromatin acetylation required for genome expression and perpetuation. Molecular cell 550 16387653
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2005 Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Genome research 409 16344560
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
1991 Cloning of cDNAs for cellular proteins that bind to the retinoblastoma gene product. Nature 324 1857421
1998 SAP30, a novel protein conserved between human and yeast, is a component of a histone deacetylase complex. Molecular cell 250 9651585
2013 The functional interactome landscape of the human histone deacetylase family. Molecular systems biology 235 23752268
2014 Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes. Journal of proteomics 215 25281560
2005 DNA-binding properties of ARID family proteins. Nucleic acids research 200 15640446
2019 H4K20me0 recognition by BRCA1-BARD1 directs homologous recombination to sister chromatids. Nature cell biology 162 30804502
2001 RBP1 recruits the mSIN3-histone deacetylase complex to the pocket of retinoblastoma tumor suppressor family proteins found in limited discrete regions of the nucleus at growth arrest. Molecular and cellular biology 160 11283269
1993 Characterization of the retinoblastoma binding proteins RBP1 and RBP2. Oncogene 156 8414517
2003 Identification and characterization of three new components of the mSin3A corepressor complex. Molecular and cellular biology 151 12724404
2003 Breast cancer metastasis suppressor 1 (BRMS1) forms complexes with retinoblastoma-binding protein 1 (RBP1) and the mSin3 histone deacetylase complex and represses transcription. The Journal of biological chemistry 141 14581478
1999 RBP1 recruits both histone deacetylase-dependent and -independent repression activities to retinoblastoma family proteins. Molecular and cellular biology 135 10490602
2022 Human transcription factor protein interaction networks. Nature communications 123 35140242
2009 The cyst nematode SPRYSEC protein RBP-1 elicits Gpa2- and RanGAP2-dependent plant cell death. PLoS pathogens 123 19714238
2015 Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes. Molecular systems biology 120 25609649
2021 Combinatorial CRISPR screen identifies fitness effects of gene paralogues. Nature communications 106 33637726
1995 The Drosophila SR protein RBP1 contributes to the regulation of doublesex alternative splicing by recognizing RBP1 RNA target sequences. The EMBO journal 104 7664738
2018 The multi-subunit GID/CTLH E3 ubiquitin ligase promotes cell proliferation and targets the transcription factor Hbp1 for degradation. eLife 102 29911972
2018 Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei. Molecular & cellular proteomics : MCP 101 30021884
2014 Exome sequencing reveals frequent inactivating mutations in ARID1A, ARID1B, ARID2 and ARID4A in microsatellite unstable colorectal cancer. International journal of cancer 101 24382590
2022 EZH2 depletion potentiates MYC degradation inhibiting neuroblastoma and small cell carcinoma tumor formation. Nature communications 99 35013218
2017 Mammalian APE1 controls miRNA processing and its interactome is linked to cancer RNA metabolism. Nature communications 99 28986522
2020 Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism. Nature metabolism 92 32694731
1992 The Drosophila RNA-binding protein RBP1 is localized to transcriptionally active sites of chromosomes and shows a functional similarity to human splicing factor ASF/SF2. Genes & development 88 1340470
2006 Deficiency of Rbbp1/Arid4a and Rbbp1l1/Arid4b alters epigenetic modifications and suppresses an imprinting defect in the PWS/AS domain. Genes & development 83 17043311
2008 Identification of chromatin remodeling genes Arid4a and Arid4b as leukemia suppressor genes. Journal of the National Cancer Institute 74 18728284
2017 ZMYM3 regulates BRCA1 localization at damaged chromatin to promote DNA repair. Genes & development 67 28242625
2021 Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy. Acta pharmaceutica Sinica. B 66 35256949
2008 Alterations of BRMS1-ARID4A interaction modify gene expression but still suppress metastasis in human breast cancer cells. The Journal of biological chemistry 65 18211900
1999 RBP1 induces growth arrest by repression of E2F-dependent transcription. Oncogene 60 10321733
2013 ARID4A and ARID4B regulate male fertility, a functional link to the AR and RB pathways. Proceedings of the National Academy of Sciences of the United States of America 59 23487765
1992 A system to study transcription by yeast RNA polymerase I within the chromosomal context: functional analysis of the ribosomal DNA enhancer and the RBP1/REB1 binding sites. The EMBO journal 53 1425596
2008 SIRT1 negatively regulates HDAC1-dependent transcriptional repression by the RBP1 family of proteins. Oncogene 52 18193082
2009 Methylation of CLDN6, FBN2, RBP1, RBP4, TFPI2, and TMEFF2 in esophageal squamous cell carcinoma. Oncology reports 49 19288010
2007 Expression analysis and RNA localization of PAI-RBP1 (SERBP1) in epithelial ovarian cancer: association with tumor progression. Gynecologic oncology 46 17698176
1989 Regional mapping of RBP4 to 10q23----q24 and RBP1 to 3q21----q22 in man. Somatic cell and molecular genetics 43 2928844
2006 RBP1 family proteins exhibit SUMOylation-dependent transcriptional repression and induce cell growth inhibition reminiscent of senescence. Molecular and cellular biology 37 16479010
2020 The RBP1-CKAP4 axis activates oncogenic autophagy and promotes cancer progression in oral squamous cell carcinoma. Cell death & disease 32 32587255
2005 Negative feedback regulation among SR splicing factors encoded by Rbp1 and Rbp1-like in Drosophila. The EMBO journal 32 15961996
2022 MicroRNA-20a-5p inhibits the autophagy and cisplatin resistance in ovarian cancer via regulating DNMT3B-mediated DNA methylation of RBP1. Reproductive toxicology (Elmsford, N.Y.) 30 34990753
2002 Identification and polymorphism of Plasmodium vivax RBP-1 peptides which bind specifically to reticulocytes. Peptides 28 12535708
1999 Closing in on the BPES gene on 3q23: mapping of a de Novo reciprocal translocation t(3;4)(q23;p15.2) breakpoint within a 45-kb cosmid and mapping of three candidate genes, RBP1, RBP2, and beta'-COP, distal to the breakpoint. Genomics 27 10191085
1993 An RNA-binding protein gene (RBP1) of Saccharomyces cerevisiae encodes a putative glucose-repressible protein containing two RNA recognition motifs. The Journal of biological chemistry 25 8325883
1997 In vivo analysis of the functional domains of the Drosophila splicing regulator RBP1. Proceedings of the National Academy of Sciences of the United States of America 23 8990170
1992 Cloning and sequence analysis of a rapamycin-binding protein-encoding gene (RBP1) from Candida albicans. Gene 23 1563628
2011 Role of Rbp1 in the acquired chill-light tolerance of cyanobacteria. Journal of bacteriology 22 21460086
2006 The doublesex splicing enhancer components Tra2 and Rbp1 also repress splicing through an intronic silencer. Molecular and cellular biology 22 17101798
2012 Differential expression of PAI-RBP1, C1orf142, and COTL1 in non-small cell lung cancer cell lines with different tumor metastatic potential. Journal of investigative medicine : the official publication of the American Federation for Clinical Research 21 22373659
2010 Retinoblastoma binding protein-1 (RBP1) is a Runx2 coactivator and promotes osteoblastic differentiation. BMC musculoskeletal disorders 21 20509905
2010 Cyclin-dependent kinase-mediated phosphorylation of RBP1 and pRb promotes their dissociation to mediate release of the SAP30·mSin3·HDAC transcriptional repressor complex. The Journal of biological chemistry 21 21148318
2019 The SR proteins SF2 and RBP1 regulate triglyceride storage in the fat body of Drosophila. Biochemical and biophysical research communications 19 31277943
2006 Estrogen receptor isoform-specific regulation of the retinoblastoma-binding protein 1 (RBBP1) gene: roles of AF1 and enhancer elements. The Journal of biological chemistry 19 16873370
1993 Alternative splicing of the RBP1 gene clusters in an internal exon that encodes potential phosphorylation sites. Oncogene 19 8455946
2018 Downregulation of ARID4A and ARID4B promote tumor progression and directly regulated by microRNA-30d in patient with prostate cancer. Journal of cellular biochemistry 18 29797600
2011 The evolution of the Gp-Rbp-1 gene in Globodera pallida includes multiple selective replacements. Molecular plant pathology 16 22192092
1995 A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames. Yeast (Chichester, England) 14 8619318
2014 Structural and biochemical analysis of the Hordeum vulgare L. HvGR-RBP1 protein, a glycine-rich RNA-binding protein involved in the regulation of barley plant development and stress response. Biochemistry 13 25495582
2013 Evolution and variability of Solanum RanGAP2, a cofactor in the incompatible interaction between the resistance protein GPA2 and the Globodera pallida effector Gp-RBP-1. BMC evolutionary biology 8 23601377
1996 CTBP1/RBP1, a Saccharomyces cerevisiae protein which binds to T-rich single-stranded DNA containing the 11-bp core sequence of autonomously replicating sequence, is a poly(deoxypyrimidine)-binding protein. European journal of biochemistry 8 8665950
2018 Functional haplotypes of ARID4A affect promoter activity and semen quality of bulls. Animal reproduction science 7 30195942
2010 Molecular cloning and expression analysis of Bmrbp1 , the Bombyx mori homologue of the Drosophila gene rbp1. Molecular biology reports 7 19731076
2021 Structural Insight into Chromatin Recognition by Multiple Domains of the Tumor Suppressor RBBP1. Journal of molecular biology 6 34506790
1999 Cytotoxic T lymphocytes that recognize decameric peptide sequences of retinoblastoma binding protein 1 (RBP-1) associated with human breast cancer. British journal of cancer 6 10496363
2023 Coronary artery disease-associated immune gene RBP1 and its pan-cancer analysis. Frontiers in cardiovascular medicine 5 36970364
2010 Screening genes of the visual cycle RGR, RBP1 and RBP3 identifies rare sequence variations. Ophthalmic genetics 5 21067480
2023 ELOA promotes tumor growth and metastasis by activating RBP1 in gastric cancer. Cancer medicine 4 37694492
2022 A novel heterozygous missense variant of the ARID4A gene identified in Han Chinese families with schizophrenia-diagnosed siblings that interferes with DNA-binding activity. Molecular psychiatry 4 35365808
2018 Crystal structure of chromo barrel domain of RBBP1. Biochemical and biophysical research communications 4 29408527
2013 ¹H, ¹³C, ¹⁵N backbone and side chain NMR resonance assignments for the N-terminal RNA recognition motif of the HvGR-RBP1 protein involved in the regulation of barley (Hordeum vulgare L.) senescence. Biomolecular NMR assignments 3 23417794
2025 CircSPINT2 confers sensitivity to osimertinib via hsa-miR-1296-3p/RBP1 axis and inhibits NSCLC progression. Molecular therapy. Oncology 2 41035422
2019 Resonance assignments for the tandem PWWP-ARID domains of human RBBP1. Biomolecular NMR assignments 2 30666492
2016 The 5' untranslated region of the rbp1 mRNA is required for translation of its mRNA under low temperatures in the cyanobacterium Synechococcus elongatus. Archives of microbiology 1 27449001
2026 Knockdown of the fly spliceosome component Rbp1 (orthologue of SRSF1) extends lifespan. GeroScience 0 41514095
2025 Arid4a Suppresses Breast Tumor Metastasis by Enhancing MTSS1 Expression via mRNA Stability. Cancer medicine 0 40066676
1997 Assignment of the cellular retinol binding protein 1 (Rbp1) and hepatic lipase (Lipc) genes to rat chromosome 8. Folia biologica 0 9338114