Affinage

Showing CDC27 — APC3 is a alias.

CDC27

Cell division cycle protein 27 homolog · UniProt P30260

Length
824 aa
Mass
91.9 kDa
Annotated
2026-06-09
65 papers in source corpus 27 papers cited in narrative 27 extracted findings
Cross-family judge vs UniProt: tie faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CDC27 (APC3) is a core, structurally essential subunit of the anaphase-promoting complex/cyclosome (APC/C), a regulated ubiquitin-protein ligase that catalyzes mitosis-specific polyubiquitination of cyclin B and other cell cycle regulators to drive the metaphase-to-anaphase transition (PMID:7736580). Genetic loss of CDC27 orthologs arrests cells at a metaphase-like stage with elevated cyclins A and B across fission yeast, Drosophila, and trypanosomes, confirming its conserved and essential role in promoting anaphase (PMID:3283148, PMID:12953067, PMID:15994309). CDC27 is built from tandem tetratricopeptide (TPR) repeats that form a superhelix (PMID:2297790), and crystallographic work establishes that this TPR domain mediates an evolutionarily conserved, functionally essential homo-dimerization shared with the related APC/C subunit Cdc16/APC6 (PMID:20206185, PMID:20924356). Within the assembled complex CDC27 serves as a structural hub: its C-terminal IR-tail binding cleft recruits the coactivators CDC20 and CDH1 and is required for APC/C-catalyzed ubiquitination, while APC16 binds asymmetrically to the symmetric CDC27 dimer and bridges APC7 into the complex (PMID:25490258); stable incorporation of CDC27 itself depends on the accessory subunit Swm1/Apc13 (PMID:15060174). CDC27 activity and abundance are governed by multiple post-translational and regulatory inputs: Cdk1 phosphorylation at consensus sites directs its chromosomal localization and is required for full APC/C function (PMID:17519285), dephosphorylated CDC27 specifically associates with kinetochores and chromosome arms during mitosis (PMID:12429948), casein kinase II phosphorylation links TGF-β signaling to APC/C-driven SnoN degradation (PMID:21209074), and O-GlcNAcylation by OGT stabilizes CDC27 by blocking autophagy-lysosomal degradation (PMID:39984622). Through APC/C-CDH1 it directs degradation of cyclin D1, cyclin B1, Skp2 and Plk-1 (PMID:20439707), and its expression and translation are controlled transcriptionally by C/EBPδ (PMID:20439707) and post-transcriptionally by HNRNP E1, whose loss causes premature APC/C activation and aneuploidy (PMID:27102006). Zebrafish cdc27 knockout produces craniofacial and skeletal malformations resembling hemifacial microsomia via reduced neural crest proliferation acting upstream of the p21/p53 pathway (PMID:38731925).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1988 High

    Established that the CDC27 ortholog is an essential nuclear factor required for mitotic spindle elongation, the first link of the gene to anaphase progression.

    Evidence Temperature-sensitive mutant analysis and nuclear fractionation of S. pombe nuc2+

    PMID:3283148

    Open questions at the time
    • Molecular activity unknown at this stage
    • No connection yet to ubiquitination or a defined complex
  2. 1990 Medium

    Defined the architecture of CDC27 as a TPR-repeat superhelix, identifying the structural module that would later mediate complex assembly and dimerization.

    Evidence Circular dichroism, limited proteolysis and in vitro DNA-binding of S. pombe nuc2+ protein

    PMID:2297790

    Open questions at the time
    • Functional role of TPR repeats not yet established
    • In vitro AT-rich DNA binding of uncertain physiological relevance
  3. 1995 High

    Answered what CDC27 does biochemically by showing it is a subunit of the 20S APC ubiquitin ligase required for cyclin B ubiquitination, defining its core enzymatic context.

    Evidence Immunodepletion and immunopurification from Xenopus mitotic extracts with in vitro ubiquitination reconstitution

    PMID:7736580

    Open questions at the time
    • Did not define CDC27's specific role within the complex
    • Substrate range beyond cyclin B not addressed
  4. 1996 Medium

    Extended CDC27 function beyond mitotic exit, showing it restricts DNA replication to once per cycle via APC/C-mediated proteolysis.

    Evidence Genetic screen and flow cytometry of S. cerevisiae conditional alleles

    PMID:8620535

    Open questions at the time
    • S-phase initiator substrate not identified
    • Mechanism inferred from DNA overreplication phenotype
  5. 1998 Medium

    Revealed a destruction-box-independent APC/C degradation route by which CDC27 controls coactivator Cdc20 stability, expanding regulatory logic of the complex.

    Evidence Yeast genetics and protein stability assays with CDC27/CDC23 mutants

    PMID:9651679

    Open questions at the time
    • Degron and recruitment mechanism undefined
    • Single-organism evidence
  6. 2002 Medium

    Linked CDC27 phosphorylation state to spatial regulation, showing dephosphorylated CDC27 associates with kinetochores and chromosomes and is required to maintain metaphase.

    Evidence Immunofluorescence, chromosome fractionation, in vitro phosphorylation and antibody microinjection in human cells

    PMID:12429948

    Open questions at the time
    • Responsible kinases/phosphatases not fully defined
    • Functional consequence of chromosomal pool unclear
  7. 2007 Medium

    Identified Cdk1 phosphorylation sites required for CDC27 chromosomal localization and APC/C function in vivo, connecting mitotic kinase activity to CDC27 targeting.

    Evidence Site-directed mutagenesis, GFP live imaging and rescue in Drosophila embryos

    PMID:17519285

    Open questions at the time
    • Whether human CDC27 uses identical sites not addressed here
    • Link between localization and catalytic activity correlative
  8. 2010 High

    Resolved the structural basis of CDC27 function, showing TPR-mediated homo-dimerization is conserved and essential and is shared with the APC6 subunit.

    Evidence X-ray crystallography of E. cuniculi Cdc27 and S. pombe Cdc16, with mutagenesis and in vivo complementation

    PMID:20206185 PMID:20924356

    Open questions at the time
    • Did not resolve coactivator-binding interface
    • Human complex architecture inferred from fungal/microsporidian structures
  9. 2014 High

    Defined CDC27 as the coactivator-recruiting hub by mapping its IR-tail binding cleft and its asymmetric engagement of APC16/APC7, explaining how it scaffolds the active ligase.

    Evidence Crystallography of human APC3 alone and with APC16, plus in vitro ubiquitination assays

    PMID:25490258

    Open questions at the time
    • Phosphorylation loop function in vivo not resolved (dispensable in vitro)
    • Dynamics of coactivator exchange not addressed
  10. 2011 High

    Established phosphorylation-dependent and signaling-coupled regulation of CDC27 through MCPH1 BRCT binding and CKII-mediated TGF-β linkage to APC/C activation.

    Evidence Crystallography/Co-IP of MCPH1–CDC27 and shRNA/phospho-mutant analysis of CKII–TGF-β–SnoN axis

    PMID:21209074 PMID:22139841

    Open questions at the time
    • Cellular consequence of MCPH1–CDC27 binding not fully defined
    • CKII regulation single-lab
  11. 2016 Medium

    Placed CDC27 under transcriptional and translational control coupled to cell cycle fidelity, showing C/EBPδ induction and HNRNP E1 silencing govern its level to prevent aneuploidy.

    Evidence siRNA/KO, shRNA, ubiquitination and cell cycle/aneuploidy assays in mammalian cells

    PMID:20439707 PMID:27102006

    Open questions at the time
    • Causal chain to specific substrates partly correlative
    • Single-lab observations
  12. 2021 Medium

    Identified O-GlcNAcylation by OGT as a stabilizing modification that protects CDC27 from autophagy-lysosomal degradation, adding a metabolic input to CDC27 abundance.

    Evidence Mass spectrometry, IP, OGT siRNA and OSMI-1 inhibition in multiple myeloma cells

    PMID:39984622

    Open questions at the time
    • GlcNAcylation sites not mapped
    • Single cell-type context
  13. 2024 Medium

    Defined a developmental requirement for CDC27, showing its loss causes craniofacial/skeletal malformation through reduced neural crest proliferation acting upstream of p21/p53.

    Evidence CRISPR knockout, in situ hybridization, PH3/TUNEL, RNA-seq and epistasis rescue in zebrafish

    PMID:38731925

    Open questions at the time
    • Whether phenotype reflects APC/C ubiquitination activity not directly tested
    • Causal substrate linking CDC27 to p21/p53 not identified

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the diverse regulatory inputs to CDC27 (Cdk1, CKII, O-GlcNAc, transcriptional/translational control) are integrated to tune APC/C activity in specific physiological and disease contexts remains unresolved.
  • No unified quantitative model of CDC27 regulation
  • Mendelian disease causation in humans not established in this corpus
  • Substrate selectivity contributions of CDC27 versus coactivators incompletely defined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 3 GO:0140096 catalytic activity, acting on a protein 3 GO:0016874 ligase activity 2 GO:0060090 molecular adaptor activity 1
Localization
GO:0005634 nucleus 2 GO:0005694 chromosome 2
Pathway
R-HSA-1640170 Cell Cycle 4 R-HSA-392499 Metabolism of proteins 2 R-HSA-1266738 Developmental Biology 1 R-HSA-162582 Signal Transduction 1 R-HSA-69306 DNA Replication 1
Partners
APC16CDC16CDC20CDH1/FZR1MAD2L2MCPH1PP5RB1
Complex memberships
APC/C (anaphase-promoting complex/cyclosome)

Evidence

Reading pass · 27 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1995 CDC27 (APC3) is a core subunit of the 20S anaphase-promoting complex (APC), which functions as a regulated ubiquitin-protein ligase that catalyzes mitosis-specific ubiquitin conjugation to cyclin B, targeting it for proteasomal destruction. CDC27 antibody depletion abolishes APC ubiquitin-ligase activity, while immunopurified CDC27 complexes reconstitute cyclin B ubiquitination in vitro. Biochemical fractionation of Xenopus mitotic egg extracts, immunodepletion with CDC27 antibodies, immunopurification, in vitro ubiquitination reconstitution assay Cell High 7736580
1988 The S. pombe nuc2+ gene (ortholog of CDC27) encodes a nuclear scaffold-associated protein required for mitotic spindle elongation; its temperature-sensitive mutation arrests cells at a metaphase-like stage with a short spindle, and the protein co-fractionates with nuclei and insoluble nuclear scaffold. Temperature-sensitive mutant analysis, cellular fractionation, immunoblotting, Percoll gradient centrifugation The Journal of cell biology High 3283148
1990 The nuc2+ protein (S. pombe CDC27 ortholog) contains ten 34-amino acid tetratricopeptide repeat (TPR) segments forming a 'snap helix' secondary structure with knob-and-hole helix-associating motifs, and also contains a domain capable of binding AT-rich DNA in vitro. Circular dichroism, limited proteolysis, model fitting, in vitro DNA-binding assay Cell Medium 2297790
1997 The serine/threonine phosphatase PP5 physically interacts with CDC16 and CDC27, two TPR-containing APC/C subunits, via the N-terminal TPR domain of PP5; the binding site on CDC16 and CDC27 maps to their C-terminal TPR block. PP5 co-localizes with the mitotic spindle apparatus by indirect immunofluorescence. Yeast two-hybrid, in vitro binding assay, deletion analysis, indirect immunofluorescence The Journal of biological chemistry Medium 9405394
1998 In budding yeast, CDC27 (together with CDC23) is required for the proteolytic instability of the APC/C coactivator Cdc20 during S phase and early mitosis; this degradation is independent of Cdc20's canonical destruction box, revealing a destruction-box-independent APC/C-mediated degradation pathway. Yeast genetics, cell cycle synchronization, protein stability assays, CDC27 and CDC23 mutant analysis Current biology : CB Medium 9651679
1996 In S. cerevisiae, CDC16 and CDC27 restrict DNA replication to once per cell cycle; conditional alleles of these genes cause accumulation of up to 8C DNA content without passage through mitosis, implicating APC/C-mediated proteolysis of an S-phase initiator in replication licensing control. Genetic screen, flow cytometry, conditional (ts) allele analysis Cell Medium 8620535
2002 The dephosphorylated form of human Cdc27/APC3 specifically associates with kinetochores and chromosome arms during mitosis (in addition to spindle poles and microtubules); only dephosphorylated Cdc27 is found on isolated chromosomes, and chromosome-associated kinases phosphorylate Cdc27 in vitro. Microinjection of anti-Cdc27 antibody arrests cells in metaphase and overrides premature anaphase induced by anti-Mad2 antibody. Immunofluorescence, chromosome isolation/fractionation, in vitro phosphorylation assay, antibody microinjection Cell cycle (Georgetown, Tex.) Medium 12429948
2010 Crystal structure of the N-terminal domain of Encephalitozoon cuniculi Cdc27 reveals a homo-dimeric TPR superhelix; mutation of the dimer interface destabilizes the protein, disrupts dimerization in solution, and abolishes complementation of S. cerevisiae cdc27 deletion in vivo, establishing that homo-dimerization via TPR motifs is evolutionarily conserved and functionally essential. X-ray crystallography, solution biophysics, site-directed mutagenesis, in vivo complementation assay Journal of molecular biology High 20206185
2010 Crystal structure of S. pombe Cdc16/Cut9 (APC6) in complex with Hcn1 (Cdc26) shows Cdc16 forms a contiguous 14-TPR superhelix; its N-terminal TPR block mediates homo-dimerization through an interface structurally related to the Cdc27 dimerization domain, demonstrating both APC/C TPR subunits use a conserved homo-dimerization mechanism. The N-acetyl-Met of Hcn1 is enclosed within the Cut9 superhelix, protecting it from Doa10 ubiquitin ligase recognition. X-ray crystallography The EMBO journal High 20924356
2014 Crystal structure of human APC3 (CDC27) alone and in complex with the C-terminal domain of APC16 reveals: (1) one APC16 binds asymmetrically to the symmetric APC3 dimer; (2) APC3's C-terminal IR-tail binding cleft is critical for coactivator (CDC20/CDH1) recruitment and APC/C-catalyzed ubiquitination; (3) APC16 recruits APC7 to APC3; (4) APC3's >200-residue phosphorylation loop is not required for ubiquitination in vitro. X-ray crystallography, biochemical ubiquitination assay, deletion/mutation analysis Journal of molecular biology High 25490258
2004 Swm1/Apc13 stabilizes the association of Cdc16 and Cdc27 within the APC/C; deletion of SWM1 reduces the stable incorporation of these subunits into the complex and abolishes APC/C ubiquitin-ligase activity in vitro. Yeast genetics, co-immunoprecipitation, in vitro ubiquitination assay Molecular and cellular biology Medium 15060174
2011 Microcephalin (MCPH1) C-terminal tandem BRCT domains bind CDC27 in a phosphorylation-dependent manner; crystal structure of MCPH1 C-BRCTs in complex with a phosphorylated CDC27 peptide defines the binding interface, and single amino acid mutations at this interface disrupt the interaction in vitro and in vivo. Co-immunoprecipitation, in vitro binding assay, X-ray crystallography, site-directed mutagenesis The Journal of biological chemistry High 22139841
2011 Phosphorylation of CDC27 by casein kinase II (CKII) in response to TGF-β signaling facilitates APC/C activation and subsequent SnoN degradation; shRNA depletion of CKII abolishes TGF-β-induced CDC27 phosphorylation and SnoN destruction, and a phosphorylation-resistant CDC27 S154A mutant attenuates TGF-β-induced growth inhibition. shRNA knockdown, site-directed mutagenesis (S154A), phosphorylation assay, co-immunoprecipitation, ubiquitination assay The Journal of biological chemistry Medium 21209074
2010 C/EBPδ transcriptionally induces CDC27 (APC3) expression, which leads to APC/C-Cdh1-mediated polyubiquitination and proteasomal degradation of cyclin D1 (as well as cyclin B1, Skp2, and Plk-1); C/EBPδ-knockout MEFs have reduced Cdc27 and elevated cyclin D1, and silencing of CDC27 or Cdh1 in breast epithelial cells increases cyclin D1 protein levels. siRNA knockdown, C/EBPδ knockout MEFs, Western blotting, co-immunoprecipitation, ubiquitination assay Proceedings of the National Academy of Sciences of the United States of America Medium 20439707
2016 The RNA-binding protein HNRNP E1 (PCBP1) post-transcriptionally silences CDC27 translation in normal mammary epithelial cells; shRNA-mediated knockdown of HNRNP E1 leads to constitutive CDC27 expression, premature activation of the G2-M APC/C-CDC20 complex, aberrant degradation of CDH1/FZR1, and consequent mitotic aberrations and aneuploidy. shRNA knockdown, Western blotting, cell cycle analysis, aneuploidy scoring, co-immunoprecipitation Molecular cancer research : MCR Medium 27102006
2003 The Drosophila mákos (mks) gene encodes the Cdc27 subunit of APC/C; mks mutant neuroblasts arrest in a metaphase-like state with elevated cyclin A and cyclin B. Loss of mks enhances centrosomal defects in polo hypomorphs (reduced γ-tubulin and CP190 at spindle poles). The metaphase arrest of mks mutants is not suppressed by bub1 spindle checkpoint mutations (confirming the checkpoint must act through APC/C), but is suppressed by mutations in twins/aar (PP2A regulatory subunit B55), indicating PP2A acts downstream of APC/C to initiate anaphase. Drosophila genetics, epistasis analysis (double mutants), immunofluorescence, immunohistochemistry Journal of cell science Medium 12953067
2007 Two Cdk1 phosphorylation consensus sites on Drosophila Cdc27 (at P304 and P456) are jointly required for chromosomal localization of GFP::Cdc27 during mitosis and for APC/C function in vivo; the double proline-to-alanine mutant (P304A,P456A) fails to localize to mitotic chromosomes and cannot rescue cdc27 mutant phenotypes, while single mutants remain functional. Site-directed mutagenesis, GFP live imaging in Drosophila syncytial embryos, in vivo rescue assay, immunostaining for cyclin B and A Journal of cell science Medium 17519285
2012 Curcumin directly binds to and crosslinks CDC27/APC3, inhibiting APC/C ubiquitin ligase activity; cells with phosphorylated CDC27 (found in highly proliferating cells) are preferentially driven to apoptosis by curcumin treatment. Pull-down assay (curcumin-bead binding), in vitro APC/C ubiquitination assay, cell cycle profiling BMC cancer Medium 22280307
2005 In Trypanosoma brucei, depletion of CDC27 by RNAi arrests procyclic-form cells in metaphase (two kinetoplasts, short metaphase-like spindle) and bloodstream-form cells in late anaphase, demonstrating an essential role of CDC27/APC/C in promoting anaphase in both life-cycle stages. RNA interference, FACS cell cycle analysis, fluorescence microscopy The Journal of biological chemistry Medium 15994309
2016 CDC27 knockdown in colorectal cancer cells causes G1/S arrest through accumulation of p21 (Cip1/Waf1), mediated via downregulation of ID1; CDC27 overexpression reduces p21 and promotes G1/S progression through ID1-dependent p21 suppression. shRNA knockdown, CDC27 overexpression, cell cycle analysis, Western blotting, rescue assays Cell death & disease Medium 26821069
2023 In cisplatin-treated human cancer cells, Mad2B (Mad2L2) exists in a basal complex with APC/C subunit CDC27, DNA polymerase ζ (Rev3/Rev1); upon cisplatin-induced DNA damage, CDC20 is additionally recruited to this complex and APC/C ubiquitination activity is enhanced in a Mad2B-dependent manner. Co-immunoprecipitation, in vitro ubiquitination assay, fluorescence microscopy (focus recruitment) The Korean journal of physiology & pharmacology Medium 37641805
2025 CDC27 is O-GlcNAcylated by OGT (O-GlcNAc transferase) in multiple myeloma cells; O-GlcNAcylation stabilizes CDC27 protein by blocking its degradation through the autophagy-lysosome pathway. Pharmacological inhibition of O-GlcNAcylation (OSMI-1) reduces CDC27 protein levels identified by mass spectrometry and immunoprecipitation. Mass spectrometry, immunoprecipitation, siRNA knockdown of OGT, pharmacological inhibition/accumulation of O-GlcNAcylation, Western blotting Acta pharmacologica Sinica Medium 39984622
1995 Human CDC27 (H-NUC) is a nuclear protein that binds to hypophosphorylated retinoblastoma protein (Rb) via two distinct regions: one overlapping the binding motif for SV40 large T antigen, and a second involving CDC27's TPR motifs. A temperature-sensitive point mutant (G640D) of CDC27 loses Rb binding at restrictive temperature. Co-immunoprecipitation, in vitro binding assay, temperature-sensitive mutagenesis, subcellular fractionation, DNA-binding assay Cell growth & differentiation Medium 7756179
2016 Cdc27 interacts with Elmo1 at the C-terminal region of Elmo1 that is essential for Dock1 association; the Elmo1-Dock1 interaction abrogates Elmo1-Cdc27 binding, but Cdc27 levels do not affect Dock1-Elmo1 interaction or Elmo1-Dock1-Rac-mediated phagocytosis. Yeast two-hybrid, co-immunoprecipitation in mammalian cells, domain mapping Biochemical and biophysical research communications Low 26882976
2022 Nuclear-localized CTEN associates with the CDC27 gene promoter and transcriptionally activates CDC27 expression; CTEN overexpression with nuclear localization signals increases CDC27 mRNA and promoter activity, and CDC27 knockdown suppresses the CTEN-mediated increase in cell migration. ChIP assay, luciferase reporter assay, shRNA knockdown, RT-qPCR, migration assay Journal of physiology and biochemistry Low 36399312
2024 cdc27 knockout in zebrafish causes craniofacial malformation, spine deformity, and cardiac edema mirroring hemifacial microsomia phenotypes; mechanistically, loss of cdc27 reduces neural crest cell (NCC) proliferation in pharyngeal arches and disrupts chondrocyte differentiation. Rescue by cdc27 mRNA injection or cdkn1a/tp53 knockout (but only partial rescue by sox9a mRNA) places cdc27 upstream of the p21/p53 pathway in pharyngeal arch development. CRISPR/Cas9 knockout in zebrafish, in situ hybridization, immunofluorescence (PH3), TUNEL assay, RNA sequencing, mRNA injection rescue, genetic epistasis International journal of molecular sciences Medium 38731925
2024 SIX1 promotes nasopharyngeal carcinoma progression through the CDC27/cyclin B1 axis; SIX1 knockdown reduces CDC27 and cyclin B1 levels, and SIX1 overexpression increases them. The E3 ligase ITCH ubiquitinates SIX1 to promote its degradation, thereby suppressing CDC27-cyclin B1-driven tumor phenotypes. siRNA knockdown, Western blotting, co-immunoprecipitation, cell proliferation/migration/invasion assays Scientific reports Low 39406717

Source papers

Stage 0 corpus · 65 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1995 A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B. Cell 844 7736580
1990 Snap helix with knob and hole: essential repeats in S. pombe nuclear protein nuc2+. Cell 271 2297790
1999 Cloning genes encoding MHC class II-restricted antigens: mutated CDC27 as a tumor antigen. Science (New York, N.Y.) 242 10334988
1988 A temperature-sensitive mutation of the Schizosaccharomyces pombe gene nuc2+ that encodes a nuclear scaffold-like protein blocks spindle elongation in mitotic anaphase. The Journal of cell biology 206 3283148
1998 The regulation of Cdc20 proteolysis reveals a role for APC components Cdc23 and Cdc27 during S phase and early mitosis. Current biology : CB 197 9651679
1996 The fission yeast Cdc1 protein, a homologue of the small subunit of DNA polymerase delta, binds to Pol3 and Cdc27. The EMBO journal 88 8887553
1996 The yeast CDC16 and CDC27 genes restrict DNA replication to once per cell cycle. Cell 80 8620535
1997 The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex. The Journal of biological chemistry 67 9405394
2010 C/EBP{delta} targets cyclin D1 for proteasome-mediated degradation via induction of CDC27/APC3 expression. Proceedings of the National Academy of Sciences of the United States of America 65 20439707
2010 The APC/C subunit Cdc16/Cut9 is a contiguous tetratricopeptide repeat superhelix with a homo-dimer interface similar to Cdc27. The EMBO journal 64 20924356
2015 MiR-27a modulates radiosensitivity of triple-negative breast cancer (TNBC) cells by targeting CDC27. Medical science monitor : international medical journal of experimental and clinical research 62 25943633
2004 Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27. Molecular and cellular biology 62 15060174
1993 Linking yeast genetics to mammalian genomes: identification and mapping of the human homolog of CDC27 via the expressed sequence tag (EST) data base. Proceedings of the National Academy of Sciences of the United States of America 58 8234252
1990 In vitro reactivation of spindle elongation in fission yeast nuc2 mutant cells. The Journal of cell biology 57 2404993
1995 Fission yeast TPR-family protein nuc2 is required for G1-arrest upon nitrogen starvation and is an inhibitor of septum formation. Journal of cell science 56 7622618
2014 Staphylococcus aureus Nuc2 is a functional, surface-attached extracellular nuclease. PloS one 54 24752186
2000 Essential interaction between the fission yeast DNA polymerase delta subunit Cdc27 and Pcn1 (PCNA) mediated through a C-terminal p21(Cip1)-like PCNA binding motif. The EMBO journal 53 10698951
2016 Downregulation of CDC27 inhibits the proliferation of colorectal cancer cells via the accumulation of p21Cip1/Waf1. Cell death & disease 46 26821069
2002 The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis. Cell cycle (Georgetown, Tex.) 45 12429948
2012 Anaphase-promoting complex/cyclosome protein Cdc27 is a target for curcumin-induced cell cycle arrest and apoptosis. BMC cancer 44 22280307
2002 The influence of the Cdc27 subunit on the properties of the Schizosaccharomyces pombe DNA polymerase delta. The Journal of biological chemistry 42 12124382
2001 The human homologue of fission Yeast cdc27, p66, is a component of active human DNA polymerase delta. Journal of biochemistry 39 11328591
2021 The importance of CDC27 in cancer: molecular pathology and clinical aspects. Cancer cell international 36 33750395
2014 Structure of an APC3-APC16 complex: insights into assembly of the anaphase-promoting complex/cyclosome. Journal of molecular biology 31 25490258
2010 Molecular structure of the N-terminal domain of the APC/C subunit Cdc27 reveals a homo-dimeric tetratricopeptide repeat architecture. Journal of molecular biology 31 20206185
2018 CDC27 Facilitates Gastric Cancer Cell Proliferation, Invasion and Metastasis via Twist-Induced Epithelial-Mesenchymal Transition. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 30 30308498
2023 LncRNA TUG1 promotes pulmonary fibrosis progression via up-regulating CDC27 and activating PI3K/Akt/mTOR pathway. Epigenetics 29 36994860
2020 Hsa_circ_0044226 knockdown attenuates progression of pulmonary fibrosis by inhibiting CDC27. Aging 29 32710728
2005 Depletion of anaphase-promoting complex or cyclosome (APC/C) subunit homolog APC1 or CDC27 of Trypanosoma brucei arrests the procyclic form in metaphase but the bloodstream form in anaphase. The Journal of biological chemistry 29 15994309
1995 Identification of a human homologue of yeast nuc2 which interacts with the retinoblastoma protein in a specific manner. Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 29 7756179
2018 Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis. Scientific reports 28 29618731
2003 Mutations in mákos, a Drosophila gene encoding the Cdc27 subunit of the anaphase promoting complex, enhance centrosomal defects in polo and are suppressed by mutations in twins/aar, which encodes a regulatory subunit of PP2A. Journal of cell science 26 12953067
1996 NUC-2, a component of the phosphate-regulated signal transduction pathway in Neurospora crassa, is an ankyrin repeat protein. Molecular & general genetics : MGG 26 8917314
2017 CDC27 Induces Metastasis and Invasion in Colorectal Cancer via the Promotion of Epithelial-To-Mesenchymal Transition. Journal of Cancer 25 28900500
2011 Molecular basis for the association of microcephalin (MCPH1) protein with the cell division cycle protein 27 (Cdc27) subunit of the anaphase-promoting complex. The Journal of biological chemistry 23 22139841
2016 PCBP1/HNRNP E1 Protects Chromosomal Integrity by Translational Regulation of CDC27. Molecular cancer research : MCR 21 27102006
1992 Molecular cloning and sequence analysis of cdc27+ required for the G2-M transition in the fission yeast Schizosaccharomyces pombe. Molecular & general genetics : MGG 21 1538696
2004 Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase delta subunit Cdc27. BMC molecular biology 19 15579205
2013 Low cdc27 and high securin expression predict short survival for breast cancer patients. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 18 23755904
2011 Phosphorylation of the anaphase-promoting complex/Cdc27 is involved in TGF-beta signaling. The Journal of biological chemistry 18 21209074
2007 Identification of genes displaying differential expression in the nuc-2 mutant strain of the mold Neurospora crassa grown under phosphate starvation. FEMS microbiology letters 17 17229059
2020 Ustilago maydis secreted T2 ribonucleases, Nuc1 and Nuc2 scavenge extracellular RNA. Cellular microbiology 16 32844528
2017 mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27. Oncotarget 16 27974673
2007 Cdk1 phosphorylation sites on Cdc27 are required for correct chromosomal localisation and APC/C function in syncytial Drosophila embryos. Journal of cell science 16 17519285
2020 CDC27 Promotes Tumor Progression and Affects PD-L1 Expression in T-Cell Lymphoblastic Lymphoma. Frontiers in oncology 14 32391258
1991 Evidence that an endo-exonuclease controlled by the NUC2 gene functions in the induction of 'petite' mutations in Saccharomyces cerevisiae. Current genetics 9 1657413
2000 Hypomorphic bimA(APC3) alleles cause errors in chromosome metabolism that activate the DNA damage checkpoint blocking cytokinesis in Aspergillus nidulans. Genetics 8 10628978
2024 Functional and Genetic Analyses Unveil the Implication of CDC27 in Hemifacial Microsomia. International journal of molecular sciences 7 38731925
2022 A Novel Gene CDC27 Causes SLE and Is Associated With the Disease Activity. Frontiers in immunology 7 35418986
2020 Comprehensive Analysis of CDC27 Related to Peritoneal Metastasis by Whole Exome Sequencing in Gastric Cancer. OncoTargets and therapy 7 32368092
2022 Circ_0000775 promotes the migration, invasion and EMT of hepatic carcinoma cells by recruiting IGF2BP2 to stabilize CDC27. Pathology, research and practice 6 35561648
2015 Association between polymorphisms in cdc27 and breast cancer in a Chinese population. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 6 25680405
2024 Implications of ITCH-mediated ubiquitination of SIX1 on CDC27-cyclinB1 signaling in nasopharyngeal carcinoma. Scientific reports 4 39406717
2022 Circular RNA circSLC8A1 inhibits the proliferation and invasion of glioma cells through targeting the miR-214-5p/CDC27 axis. Metabolic brain disease 4 35098413
2018 Author Correction: Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis. Scientific reports 4 30127502
2016 Identification of a novel protein interaction between Elmo1 and Cdc27. Biochemical and biophysical research communications 4 26882976
2005 CDC27 protein is involved in radiation response in squamous cell cervix carcinoma. Indian journal of biochemistry & biophysics 4 23923534
2025 Inhibition of CDC27 O-GlcNAcylation coordinates the antitumor efficacy in multiple myeloma through the autophagy-lysosome pathway. Acta pharmacologica Sinica 3 39984622
2023 Mad2B forms a complex with Cdc20, Cdc27, Rev3 and Rev1 in response to cisplatin-induced DNA damage. The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 3 37641805
1995 Localization of the human homolog of the yeast cell division control 27 gene (CDC27) proximal to ITGB3 on human chromosome 17q21.3. Somatic cell and molecular genetics 2 8619132
2022 Nuclear-localized CTEN is a novel transcriptional regulator and promotes cancer cell migration through its downstream target CDC27. Journal of physiology and biochemistry 1 36399312
2021 Corrigendum: CDC27 Promotes Tumor Progression and Affects PD-L1 Expression in T-Cell Lymphoblastic Lymphoma. Frontiers in oncology 1 33585203
2026 Genetic polymorphisms of the CDC27 gene are associated with susceptibility and outcomes of non-syndromic congenital heart disease: a bi-ethnic case-control study in Chinese populations. Frontiers in genetics 0 41757267
2025 Studies on the regulation of E3 ubiquitin ligase APC3 and its interacting proteins on the tetraspore formation and release in Gracilariopsis lemaneiformis (Rhodophyta). Plant science : an international journal of experimental plant biology 0 40169068
2025 Upregulation of TFDP1 and CDC27 Plays an Important Role in Bronchiectasis. Canadian respiratory journal 0 41458288

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