Affinage

ABCE1

ATP-binding cassette sub-family E member 1 · UniProt P61221

Length
599 aa
Mass
67.3 kDa
Annotated
2026-06-09
79 papers in source corpus 34 papers cited in narrative 33 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ABCE1 (yeast Rli1) is a universally conserved twin-cassette ATPase that serves as the central ribosome recycling factor in eukaryotes and archaea (PMID:21292982, PMID:28368393). Structurally it comprises two head-to-tail nucleotide-binding domains joined by a hinge that supports an ATP-driven clamp-like powerstroke, plus an N-terminal ferredoxin-like domain harboring two essential [4Fe-4S]²⁺ clusters that contact the NBD1 region and link cluster function to ATP-induced conformational change (PMID:18160405, PMID:15837203, PMID:17355973). Acting on post-termination ribosomes in concert with release factors, ABCE1 undergoes an ATP-occlusion-driven open-to-closed switch that dissociates ribosomal subunits and disengages the release factor; a single splitting event requires ATP binding but not hydrolysis, whereas hydrolysis at the asymmetric nucleotide sites drives ABCE1 release from the small subunit to reset the cycle (PMID:21292982, PMID:30198020, PMID:32064661). After splitting, the FeS domain rotates ~150° into a pocket on the 40S subunit, conferring anti-association activity, and ABCE1 then persists on the small subunit into 43S/48S pre-initiation complexes where it interacts with initiation factors including eIF2, eIF3, eIF5, and eIF3j, thereby coupling recycling to a new round of initiation (PMID:15277527, PMID:16421098, PMID:28368393, PMID:33289941). ATPase activation on the ribosome is promoted by the ribosomal stalk, which contacts NBD1 (PMID:30010948). The Fe-S clusters are indispensable for these activities and are matured onto apo-ABCE1 by the dedicated CIA targeting adaptor Yae1–Lto1 (human YAE1D1–ORAOV1), making ABCE1 function dependent on cellular iron supply and acutely sensitive to oxidative stress that disrupts cluster delivery (PMID:17355973, PMID:26182403, PMID:22855532). Through its recycling activity ABCE1 enforces 3' UTR regulatory programs: impaired recycling allows ribosomes to invade 3' UTRs, displacing exon junction complexes and abrogating nonsense-mediated decay, and ABCE1 likewise mediates nonstop mRNA decay (PMID:32668236, PMID:25128630). Beyond canonical recycling, ABCE1 is captured at stalled mitochondrial-membrane ribosomes where NOT4-dependent ubiquitination of ABCE1 generates poly-ubiquitin signals that initiate PINK1-dependent mitophagy (PMID:29861391), and it engages HIV-1 Gag assembly intermediates via a binding site exposed upon Gag dimerization to support immature capsid formation (PMID:16275648, PMID:21543480).

Mechanistic history

Synthesis pass · year-by-year structured walk · 17 steps
  1. 2004 High

    Established that the previously enigmatic RLI1/ABCE1 ATPase is directly required for translation, placing it physically at the 43S pre-initiation complex rather than at elongation or termination.

    Evidence Affinity co-purification, polysome profiling, dominant-negative ATP-site mutagenesis, and reporter assays in yeast

    PMID:15277527

    Open questions at the time
    • Did not distinguish a recycling versus initiation function
    • No structural basis for factor contacts
  2. 2006 High

    Extended the essential translation role to vertebrates and confirmed reciprocal binding to initiation factors, with an in vivo developmental requirement.

    Evidence In vitro translation, eIF2α/eIF5 co-IP, and Xenopus morpholino knockdown

    PMID:16421098

    Open questions at the time
    • Mechanism of how factor binding promotes translation unresolved
  3. 2005 High

    Defined the twin-NBD head-to-tail architecture with a unique hinge, providing the structural basis for an ATP-driven clamp motion in RLI/ABCE1.

    Evidence X-ray crystallography at 1.9 Å with yeast motif-mutant functional analysis

    PMID:15837203

    Open questions at the time
    • FeS domain not resolved in this construct
    • No ribosome-bound state
  4. 2007 High

    Identified the N-terminal FeS domain and the two [4Fe-4S]²⁺ clusters as essential cofactors structurally coupled to the NBD, defining ABCE1 as an iron-sulfur ATPase.

    Evidence Full-length crystal structure, EPR/Mössbauer spectroscopy, and yeast genetic complementation of coordinating cysteines

    PMID:17355973 PMID:18160405

    Open questions at the time
    • Functional role of clusters in catalysis not yet defined
    • Cluster assembly pathway unknown
  5. 2011 High

    Demonstrated that ABCE1 is the ribosome recycling factor, splitting subunits via an ATP-induced conformational switch and disengaging the release factor, with hydrolysis needed only for ABCE1 release.

    Evidence Reconstituted archaeal ribosome recycling and splitting assays with ATPase mutants and aRF1 interaction analysis

    PMID:21292982

    Open questions at the time
    • Atomic geometry of the splitting intermediate not visualized
    • Coupling of the two NBSs unresolved
  6. 2017 High

    Visualized the post-splitting state, showing a ~150° FeS-domain rotation onto the 40S that explains both anti-association and the splitting trajectory, and detected ABCE1 in native 43S complexes.

    Evidence Cryo-EM of yeast 40S-ABCE1 at 3.9 Å with mutagenesis and sedimentation

    PMID:28368393

    Open questions at the time
    • Pre-splitting conformation not captured in same study
    • Handoff to initiation factors not structurally defined
  7. 2018 High

    Dissected the allosteric ATPase cycle, assigning a low-turnover control site (NBS2) and a high-turnover catalytic site (NBS1) to sequential pre-splitting, splitting, and termination steps.

    Evidence Site-specific Walker-B ATPase kinetics and ribosome splitting assays

    PMID:30198020

    Open questions at the time
    • In vivo relevance of the two-site asymmetry not tested
  8. 2018 High

    Identified the ribosomal stalk as the activator that triggers ABCE1 ATPase on the ribosome, linking factor engagement to subunit dissociation.

    Evidence Crystal structure of the stalk-NBD1 interface plus ATPase assays and yeast complementation

    PMID:30010948

    Open questions at the time
    • Timing of stalk engagement within the recycling cycle not resolved
  9. 2020 High

    Refined the allosteric network coupling FeS/hinge rearrangements to nucleotide-site closure, and showed ABCE1 predominantly occupies early 43S/48S complexes interacting with eIF3j to gate mRNA loading.

    Evidence High-resolution cryo-EM of archaeal 30S-ABCE1 (2.8 Å) and native yeast/human pre-initiation complexes

    PMID:32064661 PMID:33289941

    Open questions at the time
    • How ABCE1 is eventually released during initiation not defined
    • eIF3j antagonism kinetics unquantified
  10. 2015 High

    Identified the dedicated CIA adaptor Yae1–Lto1 that delivers Fe-S clusters specifically to ABCE1, explaining how its essential cofactor is targeted, with human orthologs conserved.

    Evidence Interaction screens, co-IP, genetic depletion with Fe-S maturation readout, and cross-species complementation

    PMID:26182403

    Open questions at the time
    • Structure of the Yae1-Lto1-ABCE1 handoff unknown
  11. 2012 High

    Showed that oxidative stress acts primarily by disrupting Fe-S cluster supply to ABCE1, making the recycling factor the principal physiological target of ROS toxicity.

    Evidence Yeast RLI1 dosage manipulation, FeS-defective mutant, 55Fe radiolabeling, and anaerobic controls

    PMID:22855532

    Open questions at the time
    • Direct downstream translational consequence of ROS-impaired ABCE1 not measured here
  12. 2020 High

    Connected ABCE1 recycling activity to 3' UTR regulation, showing impaired recycling lets ribosomes invade 3' UTRs and abolishes NMD via the iron–Fe-S–ABCE1 axis.

    Evidence Genome-wide CRISPR/siRNA screen, ribosome profiling, NMD reporters, and iron/lysosome perturbation

    PMID:32668236

    Open questions at the time
    • Whether 3' UTR readthrough directly displaces EJCs in cis not structurally shown
  13. 2018 High

    Linked ABCE1 to mitochondrial quality control, showing NOT4-dependent ubiquitination of ribosome-bound ABCE1 at stalled mitochondrial mRNAs initiates PINK1-dependent mitophagy.

    Evidence Co-IP, ubiquitination assay, and in vivo Drosophila PINK1 genetic rescue with mitophagy imaging

    PMID:29861391

    Open questions at the time
    • Ubiquitin-acceptor sites on ABCE1 not mapped
    • How ubiquitinated ABCE1 recruits autophagy receptors unresolved
  14. 2005 High

    Implicated ABCE1 in HIV-1 immature capsid assembly through an interaction dependent on basic NC residues, with assembly-defective Gag failing to recruit ABCE1.

    Evidence Co-IP with Gag deletion/point mutants and capsid assembly assays

    PMID:16275648

    Open questions at the time
    • Direct versus indirect binding not yet distinguished
    • Function of ABCE1 in assembly not defined
  15. 2011 High

    Clarified that ABCE1 is recruited not by NC or RNA but by a binding surface exposed upon Gag dimerization, refining the assembly-intermediate model.

    Evidence Co-IP with GagZip dimerization chimeras, capsid assembly, and RNase-resistance assays

    PMID:21543480

    Open questions at the time
    • The exposed ABCE1-binding region of Gag not mapped
    • Catalytic contribution of ABCE1 to assembly unknown
  16. 2014 Medium

    Extended ABCE1's recycling role to nonstop mRNA decay, where it rescues ribosomes stalled at the 3' end.

    Evidence RNAi knockdown with NSD reporters in Drosophila cells

    PMID:25128630

    Open questions at the time
    • No in vitro reconstitution
    • Single method/lab
  17. 2021 Medium

    Suggested a moonlighting role in translation elongation, with Rli1 cooperating with the CCR4-NOT complex in codon-dependent elongation of difficult sequences.

    Evidence Ribosome profiling, co-purification, genetic epistasis, and polyarginine reporters in yeast

    PMID:34469733

    Open questions at the time
    • Direct elongation activity versus indirect recycling effect not separated
    • Single lab

Open questions

Synthesis pass · forward-looking unresolved questions
  • How ABCE1's recycling, initiation-licensing, quality-control, and ribosome-independent roles (e.g. RNase L modulation, cytoskeletal and meiotic-spindle functions) are partitioned and regulated in cells remains open.
  • Whether non-ribosomal activities require Fe-S clusters or ATPase activity is largely untested
  • No structural picture of ABCE1 release into initiation
  • Physiological hierarchy among functions undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140657 ATP-dependent activity 4 GO:0045182 translation regulator activity 3
Localization
GO:0005840 ribosome 3 GO:0005739 mitochondrion 2 GO:0005634 nucleus 1 GO:0005829 cytosol 1
Pathway
R-HSA-392499 Metabolism of proteins 4 R-HSA-8953854 Metabolism of RNA 2 R-HSA-9612973 Autophagy 1
Partners
EIF2EIF3JEIF5ERF1LTO1NOT4RIBOSOMAL STALK PROTEIN (AP1)YAE1
Complex memberships
43S pre-initiation complexYae1-Lto1 CIA targeting complex (substrate)post-splitting 40S/30S-ABCE1 complex

Evidence

Reading pass · 33 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2004 Yeast RLI1 (ABCE1) co-purifies with eIF3, eIF5, and eIF2 but not with elongation or termination factors; depletion reduces polysome content and 40S-bound eIF2/eIF1, indicating a direct role in 43S preinitiation complex assembly. Mutations in conserved ATP-hydrolysis residues are lethal; a dominant-negative NBD2 mutation inhibits translation initiation in vivo and in cell extracts. RLI1-GFP localizes to both nucleus and cytoplasm. Affinity co-purification, polysome profiling, dominant-negative mutagenesis, luciferase reporter translation assay, GFP live imaging The Journal of biological chemistry High 15277527
2006 Human ABCE1 is essential for in vitro and in vivo mRNA translation and binds eIF2α and eIF5. Inhibition of Xenopus ABCE1 arrests development at the gastrula stage, consistent with a translation block. In vitro translation assay, co-immunoprecipitation (eIF2α/eIF5 binding), Xenopus morpholino knockdown with developmental phenotype readout The Journal of biological chemistry High 16421098
2007 Crystal structure of Pyrococcus abyssi ABCE1 at 2.8 Å reveals four domains: two NBDs arranged head-to-tail via a hinge domain (suggesting tweezers-like ATP-driven powerstroke), and an N-terminal FeS domain structurally related to bacterial ferredoxins containing two [4Fe-4S] clusters. The FeS domain makes intimate contacts with the Y-loop of NBD1, suggesting a linkage between FeS function and ATP-induced conformational changes. X-ray crystallography (2.8 Å resolution) The Journal of biological chemistry High 18160405
2005 Crystal structure of P. furiosus RLI twin-cassette ATPase at 1.9 Å reveals two NBDs forming two composite active sites in a head-to-tail interface with a unique hinge domain, consistent with ATP-driven clamp-like motion; establishes the structural basis for ABC domain heterodimers in RLI/ABCE1. X-ray crystallography (1.9 Å), yeast in vivo functional analysis of motif mutants Structure High 15837203
2007 ABCE1 harbors two essential diamagnetic [4Fe-4S]²⁺ clusters: a ferredoxin-like cluster (Cys 4–7) and a unique ABCE1-type cluster (Cys 1,2,3,8). Seven of the eight conserved coordinating cysteines are essential for cell viability; mutation of Cys6 yields a [3Fe-4S]⁺ state but remains functional; a lethal Cys4 mutation is rescued by ligand swapping with an adjacent eukaryote-conserved cysteine. EPR/Mössbauer spectroscopy, biophysical characterization, yeast genetic complementation, site-directed mutagenesis The Journal of biological chemistry High 17355973
2011 ABCE1 in archaea drives ribosome recycling via an ATP-dependent conformational switch from open to closed (ATP-occluded) state, which dissociates ribosomes and disengages aRF1. ATP hydrolysis is not required for a single round of ribosome splitting but is required for ABCE1 release from the 30S subunit. The FeS cluster domain is essential for both ribosome binding and recycling. aRF1 directly interacts with ABCE1 and synergistically promotes recycling. Biochemical ribosome recycling assay, ATPase mutant analysis, direct interaction assay, conformational state analysis Proceedings of the National Academy of Sciences of the United States of America High 21292982
2017 Cryo-EM structure of yeast 40S-ABCE1 post-splitting complex at 3.9 Å shows the FeS cluster domain rotates ~150° into a binding pocket on the 40S subunit compared to the pre-splitting state. This repositioning (i) confers anti-association activity preventing 60S rejoining, and (ii) its collision trajectory with A-site factors explains the splitting mechanism. ABCE1 is also detected in native 43S initiation complexes. Cryo-electron microscopy (3.9 Å), mutational analysis of key interactions, polysome/sedimentation analysis Nature structural & molecular biology High 28368393
2018 ABCE1 undergoes an allosterically coupled ATPase cycle for ribosome recycling: ATP occlusion in the low-turnover control site II (NBS2) promotes pre-splitting complex formation and facilitates ATP engagement at the high-turnover site I (NBS1), which drives structural reorganization for ribosome splitting. ATP hydrolysis and ensuing ABCE1 release from the small subunit terminate the post-splitting complex. ATPase kinetics with site-specific Walker-B mutants, ribosome splitting assays, functional mutagenesis Life science alliance High 30198020
2020 Cryo-EM structure of archaeal 30S-ABCE1 post-splitting complex at 2.8 Å reveals how conformational rearrangements of the FeS cluster domain and hinge regions are coupled to nucleotide-binding site closure, and defines an allosteric network between the ribosome, ABCE1 regulatory domains, and the two structurally and functionally asymmetric ATP-binding sites. Cryo-electron microscopy (2.8 Å), mutational analysis, biochemical functional assays The EMBO journal High 32064661
2020 Native cryo-EM inventory of yeast and human ABCE1-containing pre-initiation complexes shows ABCE1 predominantly associates with early 43S (and also 48S) initiation complexes, adopts a novel hybrid NBD conformation, and interacts with the N-terminus of eIF3j; eIF3j occupies the mRNA entry channel via its C-terminus, providing a structural explanation for its antagonism of mRNA binding. Cryo-electron microscopy of native complexes, biochemical fractionation The EMBO journal High 33289941
2015 The yeast proteins Yae1 and Lto1 function as a target-specific CIA adaptor complex that recruits apo-Rli1 (ABCE1) to the CIA Fe-S assembly machinery for Fe-S cluster insertion. Depletion of Yae1 or Lto1 causes defective Fe-S maturation of Rli1 but not other CIA targets. Lto1 uses its conserved C-terminal tryptophan to contact the CIA targeting complex; deca-GX3 motifs in both proteins mediate their complex formation; Yae1 directly recruits Rli1. Human YAE1D1 and ORAOV1 functionally replace their yeast counterparts. Systematic protein interaction screens, co-IP, genetic depletion with Fe-S maturation assays, cross-species complementation eLife High 26182403
2018 Mitochondrial damage causes stalled translation of C-I30 mRNA on the mitochondrial outer membrane, recruiting Pelo, ABCE1, and the E3 ligase NOT4 to the ribosome/mRNA complex. NOT4 ubiquitinates ABCE1, generating poly-ubiquitin signals that recruit autophagy receptors to the MOM to initiate PINK1-dependent mitophagy. In Drosophila PINK1 mutants, these factors synergistically restore mitophagy and neuromuscular tissue integrity. Co-immunoprecipitation, ubiquitination assay, in vivo Drosophila genetics, mitophagy imaging Cell metabolism High 29861391
2020 A genome-wide screen reveals that ABCE1 activity (maintained by lysosomal acidification → transferrin-mediated iron uptake → Fe-S cluster biogenesis) is required for proper ribosome recycling; impaired ABCE1 function causes ribosomes to move into 3' UTRs, displacing exon junction complexes and abrogating nonsense-mediated decay (NMD) and other 3' UTR-directed regulatory mechanisms including miRNA- and RNA-binding-protein-mediated repression. Genome-wide CRISPR/siRNA screen, ribosome profiling, NMD reporter assays, iron/lysosome perturbation experiments Cell reports High 32668236
2005 The NC domain of HIV-1 Gag is required for interaction with endogenous primate ABCE1; specifically, basic residues in NC (not the zinc finger cysteine/histidine residues) are necessary for the Gag-ABCE1 interaction. Constructs failing to interact with ABCE1 also fail to form capsids, implicating ABCE1 in immature HIV-1 capsid assembly intermediates. Co-immunoprecipitation with deletion and point mutants, capsid assembly assays The Journal of biological chemistry High 16275648
2011 Assembly-competent HIV-1 GagZip chimeras (NC replaced by leucine zipper) form ABCE1-containing capsid assembly intermediates, whereas assembly-incompetent GagZip mutants (disrupted dimerization) do not. This demonstrates that ABCE1 does not bind NC directly or through an RNA bridge; instead, Gag dimerization (mediated by NC or zipper) exposes an ABCE1-binding domain elsewhere in Gag. Co-immunoprecipitation with GagZip chimeras, capsid assembly assays, RNase A resistance assay Journal of virology High 21543480
2012 ROS toxicity in yeast is primarily mediated through impairment of Rli1 (ABCE1) function: mild oxidative stress impairs Rli1p activity in nuclear ribosomal-subunit export; prooxidant resistance is decreased by RLI1 repression and increased by RLI1 overexpression; the dependency is abolished under anaerobic conditions and accentuated by a FeS cluster-defective Rli1 construct. ROS primarily disrupts [55Fe]S-cluster supply to Rli1p rather than damaging clusters already incorporated. Yeast genetic manipulation (RLI1 repression/overexpression), FeS cluster-defective mutant, 55Fe-radiolabeling, anaerobic conditions Molecular biology of the cell High 22855532
2014 ABCE1 plays a functional role in nonstop mRNA decay (NSD) in Drosophila cells, acting as the eukaryotic ribosome recycling factor that triggers ribosome rescue at the 3' end of nonstop mRNAs. RNAi knockdown in Drosophila cells with NSD reporter assays Biochimie Medium 25128630
2018 The ribosomal stalk protein (archaeal aP1) binds via conserved hydrophobic C-terminal residues to NBD1 of aABCE1, and this interaction is crucial for ATPase activation of ABCE1 on the ribosome and for ribosome subunit dissociation; functional role of stalk–ABCE1 interaction is conserved in yeast. Biochemical ATPase assay, crystal structure of stalk–NBD1 interaction, yeast mutagenesis/complementation Nucleic acids research High 30010948
2015 ABCE1 functions as an endogenous suppressor of RNA silencing in a conserved manner: human ABCE1 suppresses RNA silencing in N. benthamiana, HEK293 cells, and C. elegans. Co-IP/MS identified potential interactors including translin and TRAX, components that support RNAi. Cross-species RNA silencing suppression assay, co-immunoprecipitation and mass spectrometry PloS one Medium 25659154
2001 A fraction of cellular RNase L and RLI (ABCE1) is localized in mitochondria. Stable transfection of RLI sense cDNA (overexpression) or RNase L antisense prevented IFNα-induced post-transcriptional down-regulation of mitochondrial mRNAs and blocked IFNα-induced cell growth inhibition, demonstrating that ABCE1 (through inhibition of RNase L) mediates IFNα antiproliferative effects via mitochondrial mRNA degradation. Subcellular fractionation, stable transfection with RLI sense/RNase L antisense constructs, mitochondrial mRNA stability assays The Journal of biological chemistry Medium 11585831
1998 RLI (ABCE1) mRNA is induced by double-stranded RNA (poly(I):poly(C)) but not by interferon; this induction leads to inhibition of RNase L 2-5A-binding activity. RLI protein has rapid turnover, making the inhibition transient. Northern blot/Western blot, RNase L activity assay after dsRNA treatment Journal of interferon & cytokine research Medium 9877446
2021 Not5 (CCR4-NOT complex) co-purifies with ribosomes and Rli1 (ABCE1) but not with eIF5A; Not4 and Not5 modulate translation elongation in a codon-dependent manner that inversely correlates with eIF5A depletion and positively correlates with Rli1 depletion. Overexpression of wild-type or non-complementing Rli1, and loss of Rps7A ubiquitination, enable Not4 E3 ligase-dependent translation of polyarginine stretches, indicating a moonlighting function of Rli1 in elongation. Ribosome profiling, co-purification, genetic epistasis with elongation factor mutants, polyarginine translation reporter Cell reports Medium 34469733
2019 ABCE1 is specifically required for efficient translation of paramyxoviral and pneumoviral mRNAs (measles, mumps, RSV) but not for general cellular protein synthesis, indicating that these viral mRNAs exploit specific ABCE1-dependent translation mechanisms. Genome-scale siRNA screens, viral replication assays, pulse-labeling of viral vs. cellular proteins after ABCE1 knockdown mBio Medium 31088929
2021 ABCE1 regulates RNase L activity and RNase L-induced autophagy during viral infections. ABCE1 knockdown enhances 2-5A-induced RNase L activity and accelerates autophagy onset; enhanced autophagy in ABCE1-depleted cells promotes EMCV replication. ABCE1 depletion sensitizes cells to apoptosis via caspase-3-mediated cleavage of Beclin-1, switching autophagy to apoptosis. ABCE1 siRNA knockdown, RNase L activity assay, autophagy and apoptosis assays, EMCV infection Viruses Medium 33670646
2020 ABCE1 directly binds to form a complex with RNase L and accelerates RNase L dimer formation in the absence of 2-5A. Depletion of ABCE1 represses 2-5A-induced RNase L activation and stabilizes exogenous RNA, and the double depletion of both ABCE1 and RNase L does not further stabilize RNA beyond single depletion, indicating they act together in RNA decay. This positions ABCE1 as a positive regulator of RNase L-mediated exogenous RNA decay. Co-immunoprecipitation, RNase L dimerization assay, RNA stability assays, single/double siRNA depletion Viruses Medium 32033097
2017 Abce1 localizes as small dots in the nucleus at the germinal vesicle (GV) stage in mouse oocytes, disperses around the spindle after GVBD, and caps the spindle poles during anaphase/telophase. Knockdown of Abce1 by siRNA injection delays GVBD, impairs first polar body extrusion, disrupts spindle assembly and chromosome alignment, dissociates γ-tubulin and p-MAPK from spindle poles, and causes aneuploidy. Immunostaining/confocal microscopy for localization, siRNA injection with meiotic phenotype analysis, γ-tubulin and p-MAPK localization Oncotarget Medium 28380459
2019 Tip60 acetyltransferase directly acetylates ABCE1 in lung cancer cells; knockdown of Tip60 decreases ABCE1 acetylation and inhibits lung cancer cell proliferation, invasion, and migration in vitro and tumor growth in vivo. Co-immunoprecipitation, Western blot for acetylation, Tip60 siRNA knockdown, xenograft model Experimental and therapeutic medicine Medium 30936993
2016 ABCE1 interacts with β-actin (demonstrated by GST pull-down and reciprocal Co-IP). ABCE1 overexpression increases F-actin rearrangement and lung cancer cell migration; the FeS cluster domain of ABCE1 is the key domain mediating β-actin binding, and deletion of FeS clusters reverses the pro-migratory and pro-proliferative effects of ABCE1 overexpression. GST pull-down, co-immunoprecipitation, FeS deletion mutagenesis, migration assays, F-actin immunofluorescence Oncology reports / Cellular physiology and biochemistry Medium 27109616 29145194
2007 TULA proteins associate with ABCE1 (identified by mass spectrometry) and substantially inhibit production of both sub-genomic and full-length HIV-1 viral particles; this inhibitory effect depends on the UBA domain of TULA, suggesting TULA is recruited to HIV assembly sites via ABCE1 where it disrupts ubiquitylation-dependent late steps of the HIV life cycle. Mass spectrometry-based protein interaction, HIV-1 particle production assay, UBA domain mutant analysis Virology Low 18006034
2016 ABCE1 depletion by siRNA in human cells causes accumulation in S phase with inefficient DNA synthesis and reduced histone mRNA and protein levels, indicating ABCE1 is essential for histone biosynthesis and normal S phase progression beyond its general translation role. siRNA knockdown, BrdU incorporation, flow cytometry cell cycle analysis, histone mRNA/protein quantification Cell cycle Medium 26985706
2021 Human ABCE1 mRNA translation is tightly regulated by AUG upstream open reading frames (uORFs) in the 5'-leader sequence in colorectal cells: uORF1 acts as a barrier to downstream uORF translation; ribosomes bypassing uORF1/2 initiate at inhibitory uORF3 or uORF5 that repress main ORF translation. This inhibitory regulation is slightly overcome during ER stress. Luciferase reporter assays with uORF mutagenesis, ribosome profiling analysis, ER stress induction Biomedicines Medium 34440115
2025 Cryo-EM structure of the AMD1 C-tail-stalled ribosome complex captures eRF1 and ABCE1 trapped together on the ribosome; the AMD1 nascent chain C-tail forms a molecular clamp positioning an arginine finger in the peptidyl-transferase center that blocks eRF1 GGQ motif accommodation, thereby arresting translation termination at the pre-ABCE1-recycling step. Cryo-electron microscopy of ribosome-nascent chain complex, structural analysis bioRxiv (preprint)preprint Medium bio_10.1101_2025.11.16.688537
2025 Genetic epistasis in yeast using heterologous eRF3 and ABCE1 factors demonstrates that eRF3 remains bound to the ribosomal GTPase center after eRF1-mediated peptide release, awaiting ABCE1 arrival; eRF3 interacts sequentially with eRF1 and then ABCE1 during final translation termination/recycling steps. Yeast conditional double-knockout epistasis using heterologous eRF1/eRF3/ABCE1 variants, genetic complementation bioRxiv (preprint)preprint Medium bio_10.1101_2025.02.05.636767

Source papers

Stage 0 corpus · 79 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2004 The essential ATP-binding cassette protein RLI1 functions in translation by promoting preinitiation complex assembly. The Journal of biological chemistry 152 15277527
2011 Ribosome recycling depends on a mechanistic link between the FeS cluster domain and a conformational switch of the twin-ATPase ABCE1. Proceedings of the National Academy of Sciences of the United States of America 132 21292982
2006 The essential vertebrate ABCE1 protein interacts with eukaryotic initiation factors. The Journal of biological chemistry 120 16421098
2009 High antitumor activity of RLI, an interleukin-15 (IL-15)-IL-15 receptor alpha fusion protein, in metastatic melanoma and colorectal cancer. Molecular cancer therapeutics 115 19723883
2002 Catalog of 605 single-nucleotide polymorphisms (SNPs) among 13 genes encoding human ATP-binding cassette transporters: ABCA4, ABCA7, ABCA8, ABCD1, ABCD3, ABCD4, ABCE1, ABCF1, ABCG1, ABCG2, ABCG4, ABCG5, and ABCG8. Journal of human genetics 95 12111378
2007 Structural organization of essential iron-sulfur clusters in the evolutionarily highly conserved ATP-binding cassette protein ABCE1. The Journal of biological chemistry 89 17355973
2018 Ubiquitination of ABCE1 by NOT4 in Response to Mitochondrial Damage Links Co-translational Quality Control to PINK1-Directed Mitophagy. Cell metabolism 78 29861391
2018 An SPX-RLI1 Module Regulates Leaf Inclination in Response to Phosphate Availability in Rice. The Plant cell 74 29610209
2016 Enhanced Antibacterial Activity of Acinetobacter baumannii Bacteriophage ØABP-01 Endolysin (LysABP-01) in Combination with Colistin. Frontiers in microbiology 74 27656173
2005 Basic residues in the nucleocapsid domain of Gag are required for interaction of HIV-1 gag with ABCE1 (HP68), a cellular protein important for HIV-1 capsid assembly. The Journal of biological chemistry 74 16275648
2007 X-ray structure of the complete ABC enzyme ABCE1 from Pyrococcus abyssi. The Journal of biological chemistry 73 18160405
2001 The 2-5A/RNase L/RNase L inhibitor (RLI) [correction of (RNI)] pathway regulates mitochondrial mRNAs stability in interferon alpha-treated H9 cells. The Journal of biological chemistry 67 11585831
2017 Structure of the 40S-ABCE1 post-splitting complex in ribosome recycling and translation initiation. Nature structural & molecular biology 65 28368393
2005 X-ray structure of RLI, an essential twin cassette ABC ATPase involved in ribosome biogenesis and HIV capsid assembly. Structure (London, England : 1993) 61 15837203
2012 The essential iron-sulfur protein Rli1 is an important target accounting for inhibition of cell growth by reactive oxygen species. Molecular biology of the cell 60 22855532
2013 Tumor targeting of the IL-15 superagonist RLI by an anti-GD2 antibody strongly enhances its antitumor potency. International journal of cancer 58 23354868
2016 IL-15 Trans-Signaling with the Superagonist RLI Promotes Effector/Memory CD8+ T Cell Responses and Enhances Antitumor Activity of PD-1 Antagonists. Journal of immunology (Baltimore, Md. : 1950) 54 27217584
2015 The deca-GX3 proteins Yae1-Lto1 function as adaptors recruiting the ABC protein Rli1 for iron-sulfur cluster insertion. eLife 53 26182403
1998 RNase L inhibitor (RLI) antisense constructions block partially the down regulation of the 2-5A/RNase L pathway in encephalomyocarditis-virus-(EMCV)-infected cells. European journal of biochemistry 53 9660177
2020 A structural inventory of native ribosomal ABCE1-43S pre-initiation complexes. The EMBO journal 50 33289941
2020 Ribosome Recycling by ABCE1 Links Lysosomal Function and Iron Homeostasis to 3' UTR-Directed Regulation and Nonsense-Mediated Decay. Cell reports 48 32668236
2012 The biological regulation of ABCE1. IUBMB life 42 23008114
2015 MicroRNA-299-3p promotes the sensibility of lung cancer to doxorubicin through directly targeting ABCE1. International journal of clinical and experimental pathology 39 26617714
2020 IL-15 superagonist RLI has potent immunostimulatory properties on NK cells: implications for antimetastatic treatment. Journal for immunotherapy of cancer 37 32532840
2020 Modulation of nitrate-induced phosphate response by the MYB transcription factor RLI1/HINGE1 in the nucleus. Molecular plant 35 33316467
2017 Genistein suppresses retinoblastoma cell viability and growth and induces apoptosis by upregulating miR-145 and inhibiting its target ABCE1. Molecular vision 34 28706438
2021 Not4 and Not5 modulate translation elongation by Rps7A ubiquitination, Rli1 moonlighting, and condensates that exclude eIF5A. Cell reports 33 34469733
2014 Highly potent anti-CD20-RLI immunocytokine targeting established human B lymphoma in SCID mouse. mAbs 33 25072059
2020 Molecular analysis of the ribosome recycling factor ABCE1 bound to the 30S post-splitting complex. The EMBO journal 31 32064661
2012 Role of the ABCE1 gene in human lung adenocarcinoma. Oncology reports 31 22267055
2018 Down-regulation of ABCE1 inhibits temozolomide resistance in glioma through the PI3K/Akt/NF-κB signaling pathway. Bioscience reports 30 30455394
2019 Comparative Loss-of-Function Screens Reveal ABCE1 as an Essential Cellular Host Factor for Efficient Translation of Paramyxoviridae and Pneumoviridae. mBio 28 31088929
2010 A small interfering ABCE1-targeting RNA inhibits the proliferation and invasiveness of small cell lung cancer. International journal of molecular medicine 28 20372810
2004 A plant orthologue of RNase L inhibitor (RLI) is induced in plants showing RNA interference. Journal of molecular evolution 28 15383904
2016 ABCE1 plays an essential role in lung cancer progression and metastasis. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 27 26733164
2011 HIV Gag-leucine zipper chimeras form ABCE1-containing intermediates and RNase-resistant immature capsids similar to those formed by wild-type HIV-1 Gag. Journal of virology 27 21543480
2014 siRNA‑induced ABCE1 silencing inhibits proliferation and invasion of breast cancer cells. Molecular medicine reports 26 25070080
2020 Suppression of ABCE1-Mediated mRNA Translation Limits N-MYC-Driven Cancer Progression. Cancer research 22 32651259
2021 LncRNA HOTAIRM1 knockdown inhibits cell glycolysis metabolism and tumor progression by miR-498/ABCE1 axis in non-small cell lung cancer. Genes & genomics 20 33537917
2019 MiR-153 inhibits the resistance of lung cancer to gefitinib via modulating expression of ABCE1. Cancer biomarkers : section A of Disease markers 20 31306106
2007 TULA proteins bind to ABCE-1, a host factor of HIV-1 assembly, and inhibit HIV-1 biogenesis in a UBA-dependent fashion. Virology 20 18006034
2005 ABCE1, a member of ATP-binding cassette transporter gene, encodes peptides capable of inducing HLA-A2-restricted and tumor-reactive cytotoxic T lymphocytes in colon cancer patients. Oncology reports 19 15809757
2014 Depleting ABCE1 expression induces apoptosis and inhibits the ability of proliferation and migration of human esophageal carcinoma cells. International journal of clinical and experimental pathology 18 24551278
2006 Role of indirect allo- and autoreactivity in anti-tumor responses induced by recipient leukocyte infusions (RLI) in mixed chimeras prepared with nonmyeloablative conditioning. Clinical immunology (Orlando, Fla.) 18 16675304
1998 The RNase L inhibitor (RLI) is induced by double-stranded RNA. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 18 9877446
2019 Long noncoding RNA FAM201A mediates the metastasis of lung squamous cell cancer via regulating ABCE1 expression. European review for medical and pharmacological sciences 17 31841188
2015 ABCE1 is a highly conserved RNA silencing suppressor. PloS one 17 25659154
2018 Ribosome recycling is coordinated by processive events in two asymmetric ATP sites of ABCE1. Life science alliance 16 30198020
2020 MicroRNA‑145 promotes the apoptosis of leukemic stem cells and enhances drug‑resistant K562/ADM cell sensitivity to adriamycin via the regulation of ABCE1. International journal of molecular medicine 15 32945355
2014 A functional involvement of ABCE1, eukaryotic ribosome recycling factor, in nonstop mRNA decay in Drosophila melanogaster cells. Biochimie 15 25128630
2017 Abce1 orchestrates M-phase entry and cytoskeleton architecture in mouse oocyte. Oncotarget 13 28380459
2019 Tip60-siRNA regulates ABCE1 acetylation to suppress lung cancer growth via activation of the apoptotic signaling pathway. Experimental and therapeutic medicine 12 30936993
2016 ABCE1 is essential for S phase progression in human cells. Cell cycle (Georgetown, Tex.) 12 26985706
2018 The ribosomal stalk protein is crucial for the action of the conserved ATPase ABCE1. Nucleic acids research 11 30010948
2014 Knock-down of ABCE1 gene induces G1/S arrest in human oral cancer cells. International journal of clinical and experimental pathology 11 25337191
2021 ABCE1 Regulates RNase L-Induced Autophagy during Viral Infections. Viruses 10 33670646
2015 Downregulation of ABCE1 via siRNA affects the sensitivity of A549 cells against chemotherapeutic agents. Medical oncology (Northwood, London, England) 10 25744244
2016 Tumor metastatic promoter ABCE1 interacts with the cytoskeleton protein actin and increases cell motility. Oncology reports 9 27109616
2019 Dual inhibition of ABCE1 and LCP1 by microRNA-96 results in an additive effect in breast cancer mouse model. Oncotarget 8 31007850
2017 Deficiency of Functional Iron-Sulfur Domains in ABCE1 Inhibits the Proliferation and Migration of Lung Adenocarcinomas By Regulating the Biogenesis of Beta-Actin In Vitro. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 8 29145194
2010 Protein synthesis meets ABC ATPases: new roles for Rli1/ABCE1. EMBO reports 8 20154644
2020 ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay. Viruses 7 32033097
2024 Restoration of miR-299-3p promotes macrophage phagocytosis and suppresses malignant phenotypes in breast cancer carcinogenesis via dual-targeting CD47 and ABCE1. International immunopharmacology 6 38394889
2021 Translation of ABCE1 Is Tightly Regulated by Upstream Open Reading Frames in Human Colorectal Cells. Biomedicines 6 34440115
2011 Despite an impaired response to IL-7, CD4+EM T cells from HIV-positive patients proliferate normally in response to IL-15 and its superagonist, RLI. AIDS (London, England) 6 21673554
2025 ABCE1 facilitates tumour progression via aerobic glycolysis and inhibits cell death in human colorectal cancer cells through the p53 signalling pathway. Scientific reports 5 40634536
2018 The expression and correlation between chemokine CCL7 and ABCE1 in non-small cell lung cancer. Experimental and therapeutic medicine 5 30214518
2025 The RLI1-OsPUB77-OsBZR3 module mediates the crosstalk between phosphate starvation and brassinosteroid signaling pathways to shape rice shoot architecture. Molecular plant 4 41017147
2015 Effect of ABCE1-silencing gene, transfected by electrotransfer, on the proliferation, invasion, and migration of human thyroid carcinoma SW579 cells. Genetics and molecular research : GMR 4 26600528
2009 An excess of rare genetic variation in ABCE1 among Yorubans and African-American individuals with HIV-1. Genes and immunity 4 19657357
2025 A novel lncRNA ABCE1-5 regulates pulmonary fibrosis by targeting KRT14. American journal of physiology. Cell physiology 3 40111939
2023 Casein Kinase 2α Augments Oxaliplatin Resistance in Colorectal Cancer Cells by Increasing ABCE1 Expression. Anticancer research 3 37247928
2025 Genetic variation within ABCE1 is associated with the chicken L blood system. Poultry science 2 41265263
2022 The Expression and Effect of ABCE1 in Gastric Adenocarcinoma. Applied biochemistry and biotechnology 2 35737213
2022 A pan-cancer analysis of the oncogenic role of ATP binding cassette subfamily E member 1 (ABCE1) in human tumors: An observational study. Medicine 2 36401432
2026 Profound alterations of cancer transcriptomes by the RNase L inhibitor ABCE1 through the modulation of UU/UA-dinucleotide rich transcript abundance. RNA biology 0 41663214
2026 Constitutive RLI Armoring Enhances CAR-NK Cell Effector Functions but Causes Lethal Toxicity In Vivo. International journal of molecular sciences 0 42074194
2023 [Tanshinone IIA inhibits hypoxia/reoxygenation-induced cardiomyocyte apoptosis and autophagy by regulating ABCE1]. Zhonghua wei zhong bing ji jiu yi xue 0 37366130
2022 Human ABCE1 exhibits temperature-dependent heterologous co-functionality in S. cerevisiae. FEBS open bio 0 35792803

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