Affinage

ABCA1

Phospholipid-transporting ATPase ABCA1 · UniProt O95477

Round 2 corrected
Length
2261 aa
Mass
254.3 kDa
Annotated
2026-04-28
130 papers in source corpus 38 papers cited in narrative 34 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ABCA1 is an ATP-hydrolyzing phospholipid flippase that translocates phospholipids from the inner to the outer leaflet of the plasma membrane, creating exovesiculated lipid domains to which lipid-free apoA-I binds and solubilizes phospholipid and cholesterol to assemble nascent discoidal HDL particles (PMID:17604270, PMID:10918065). Its ATPase activity is stimulated by choline-containing phospholipids and inhibited by cholesterol (PMID:16500904), and the protein cycles between the plasma membrane and early endosomes via clathrin/Rab5-dependent endocytosis and Rab4-dependent recycling, with calpain-mediated degradation in endosomes serving as a major turnover mechanism retarded by apoA-I binding (PMID:19170766, PMID:21835264). Transcription is driven by LXR/RXR and RAR/RXR heterodimers acting on a DR4 promoter element, with additional regulation by HIF-1, AMPK→LXRα, TCF21, and BAI1→ELMO1→Rac1 pathways, while post-translational stability is modulated by PLD2/diacylglycerol-dependent serine phosphorylation, OSBP sterol-binding-domain-dependent destabilization, and cAMP/PKA-dependent phosphorylation that enhances efflux (PMID:10858438, PMID:14560020, PMID:27343431, PMID:16118212, PMID:18450749, PMID:12454270). Loss-of-function mutations in ABCA1 cause Tangier disease and familial HDL deficiency, and cell-type-specific deletion in pancreatic β-cells or hepatocytes disrupts cholesterol-dependent insulin secretion and insulin signaling, respectively, while brain ABCA1 deficiency reduces apoE lipidation and exacerbates amyloid deposition (PMID:10533863, PMID:17322896, PMID:28591582, PMID:16207713).

Mechanistic history

Synthesis pass · year-by-year structured walk · 20 steps
  1. 1999 High

    Identification of ABCA1 as the gene mutated in Tangier disease and familial HDL deficiency resolved a decades-old question about the molecular basis of apolipoprotein-mediated cholesterol efflux and HDL formation.

    Evidence Gene expression microarrays, genetic mapping, mutation sequencing in patient fibroblasts, and functional validation via siRNA/overexpression across multiple independent labs

    PMID:10431236 PMID:10431237 PMID:10431238 PMID:10525055 PMID:10533863 PMID:10535983

    Open questions at the time
    • Mechanism by which ABCA1 moves lipids was unknown
    • Direct protein–apoA-I interaction not yet shown
    • Transcriptional regulation undefined
  2. 2000 High

    Demonstration that apoA-I binds directly to ABCA1 at the cell surface and that ABCA1 expression induces phospholipid translocation and phosphatidylserine exposure established it as a lipid flippase generating exofacial membrane strain.

    Evidence Chemical cross-linking, co-immunoprecipitation, echinocyte morphology in ABCA1-overexpressing cells, prothrombinase assay and spin-labeled phospholipid transbilayer redistribution in knockout and transfected cells

    PMID:10878804 PMID:10918065

    Open questions at the time
    • No purified-protein ATPase data yet
    • Structural basis of apoA-I–ABCA1 interaction unknown
    • Whether flippase activity is sufficient for HDL assembly unresolved
  3. 2000 High

    Discovery that LXR/RXR heterodimers directly activate the ABCA1 promoter via a DR4 element established the sterol-sensing transcriptional circuit controlling reverse cholesterol transport.

    Evidence Promoter-reporter assays with site-directed mutagenesis of DR4 element, LXR/RXR co-transfection, rexinoid pharmacology in mice

    PMID:10858438 PMID:10968783

    Open questions at the time
    • Other transcription factors acting on ABCA1 promoter unknown
    • Post-transcriptional regulation not addressed
  4. 2002 High

    Identification of cAMP/PKA-dependent phosphorylation of ABCA1 as an activating signal for cholesterol efflux revealed a rapid post-translational regulatory mechanism distinct from transcriptional control.

    Evidence Radiolabeled phosphorylation assay with PKA inhibitor H-89 and genetic controls from Tangier disease and familial HDL deficiency fibroblasts

    PMID:12454270

    Open questions at the time
    • Specific phosphorylation sites on ABCA1 not mapped
    • Kinase-phosphatase balance not characterized
  5. 2003 High

    RAR/RXR was shown to directly activate ABCA1 via the same DR4 element as LXR/RXR, expanding the regulatory network to include retinoid signaling and demonstrating receptor redundancy at this promoter element.

    Evidence ChIP in macrophages, RARγ-knockout mouse macrophages showing compensatory RARα induction

    PMID:14560020

    Open questions at the time
    • Physiological contexts where RAR dominates over LXR unclear
    • Combinatorial versus competitive binding not resolved
  6. 2004 High

    Identification of syntaxin 13 and flotillin-1 as direct ABCA1 interaction partners in lipid raft microdomains established that ABCA1 operates within a defined membrane trafficking complex, and probucol was shown to block ABCA1 translocation to the plasma membrane.

    Evidence Reciprocal co-immunoprecipitation, siRNA knockdown of syntaxin 13 reducing ABCA1 levels and efflux, probucol trafficking assays with Tangier fibroblast controls

    PMID:15469992 PMID:15514211

    Open questions at the time
    • How syntaxin 13 controls ABCA1 stability versus trafficking not distinguished
    • Probucol's direct molecular target on ABCA1 not identified
  7. 2005 High

    Unsaturated fatty acids were shown to destabilize ABCA1 through PLD2-generated diacylglycerol and serine phosphorylation, revealing a lipid-sensing post-translational degradation pathway.

    Evidence PLD2 siRNA rescue, triacsin C inhibitor, diacylglycerol analog experiments with phosphorylation and protein stability readouts

    PMID:16118212

    Open questions at the time
    • Specific serine residues targeted not identified
    • Whether PLD2 pathway operates in vivo not tested
  8. 2006 High

    Reconstitution of purified ABCA1 into liposomes provided direct biochemical proof of intrinsic ATPase activity stimulated by phospholipids and inhibited by cholesterol, confirming the flippase hypothesis at the protein level.

    Evidence Purified human ABCA1 from Sf9 cells, liposome ATPase assay with defined lipid compositions

    PMID:16500904

    Open questions at the time
    • No direct lipid translocation demonstrated with purified protein
    • Structure of ABCA1 not solved
  9. 2006 High

    Brain ABCA1 deficiency was shown to reduce soluble apoE and increase amyloid deposition in APP transgenic mice, and HIV-1 Nef was found to bind and impair ABCA1, establishing disease-relevant consequences of ABCA1 dysfunction beyond HDL metabolism.

    Evidence ABCA1-KO × APP23 crosses with amyloid staining and apoE fractionation; Nef–ABCA1 co-IP with efflux and virion cholesterol assays

    PMID:16207713 PMID:17076584

    Open questions at the time
    • Whether ABCA1 effects on amyloid are solely via apoE lipidation not resolved
    • In vivo relevance of Nef–ABCA1 interaction in HIV patients not confirmed
  10. 2007 High

    A detailed mechanistic model of nascent HDL assembly was established: ABCA1-driven phospholipid translocation creates curved exovesiculated membrane domains from which apoA-I spontaneously solubilizes discoidal particles, and separately, β-cell-specific ABCA1 knockout revealed a role in insulin secretion via islet cholesterol homeostasis.

    Evidence Systematic lipid efflux kinetics and HDL particle composition analysis; conditional β-cell ABCA1 KO mice with glucose tolerance and insulin secretion assays

    PMID:17322896 PMID:17604270

    Open questions at the time
    • Stoichiometry and kinetics of apoA-I–membrane domain interaction not fully defined
    • Whether β-cell phenotype is relevant to human diabetes from ABCA1 variants not established
  11. 2008 High

    ABCA1 endocytic recycling via clathrin/Rab5 internalization and Rab4 return was mapped, and OSBP was identified as a sterol-sensing negative regulator of ABCA1 protein stability, integrating trafficking and post-translational control.

    Evidence Tagged ABCA1 constructs with dominant-negative Rabs and clathrin inhibition; OSBP siRNA with cycloheximide chase and domain mutants

    PMID:18450749 PMID:19170766

    Open questions at the time
    • How OSBP sterol binding leads to ABCA1 degradation mechanistically unclear
    • Relative contribution of recycling versus de novo synthesis to steady-state ABCA1 not quantified
  12. 2009 Medium

    Macrophage ABCA1 was linked to anti-inflammatory function through TLR signaling modulation, HIF-1 was identified as a direct transcriptional activator under hypoxia, and the LRP1→cPLA2→LXR pathway was shown to control ABCA1 expression, substantially expanding the upstream regulatory network.

    Evidence ABCA1/ABCG1-KO macrophages with TLR/cytokine readouts; HIF-1 response element binding and promoter assays; LRP1-KO cells with cPLA2 signaling analysis

    PMID:19718435 PMID:19797709 PMID:19828131

    Open questions at the time
    • ABCA1-specific versus ABCG1-specific anti-inflammatory contributions not fully separated
    • HIF-1 regulation of ABCA1 not independently replicated
    • LRP1 pathway is single-study evidence
  13. 2011 High

    Calpain was identified as the protease responsible for ABCA1 degradation in early endosomes, with apoA-I binding and interactors (α1-syntrophin, LXRβ, calmodulin) protecting ABCA1 from calpain, and apoA-I/ABCA1 signaling via JAK2/STAT3, PKA, CDC42, and PKC was documented.

    Evidence Calpain inhibitor pharmacology with subcellular fractionation and in vivo validation; kinase inhibitors and dominant-negative constructs for signaling pathway dissection

    PMID:21835264 PMID:21920460

    Open questions at the time
    • Calpain cleavage site on ABCA1 not mapped
    • Relative importance of individual signaling arms not established in vivo
    • JAK2/STAT3 pathway evidence largely compiled from multiple prior studies
  14. 2012 High

    miR-33a was shown to suppress ABCA1 expression in islets and reduce insulin secretion through cholesterol accumulation, providing a post-transcriptional regulatory axis linking metabolic microRNA networks to β-cell function.

    Evidence Adenoviral miR-33a overexpression and inhibition in human/mouse islets with cholesterol depletion rescue

    PMID:22315319

    Open questions at the time
    • In vivo relevance of miR-33a–ABCA1 axis to type 2 diabetes not demonstrated
    • Other miR-33a targets that could contribute not excluded
  15. 2015 High

    A sterol-independent transcriptional induction pathway was discovered: apoptotic cells activate BAI1→ELMO1→Rac1 to upregulate ABCA1 independently of LXR, linking efferocytosis directly to cholesterol efflux capacity.

    Evidence Primary macrophages from BAI1/ELMO1/Rac1-KO mice, in vivo high-fat diet model with aortic root amyloid and lipid profiling

    PMID:26075824

    Open questions at the time
    • Downstream transcription factor activated by Rac1 to drive ABCA1 not identified
    • Whether this pathway is relevant in non-macrophage cell types unknown
  16. 2016 High

    AMPK was placed upstream of LXRα in ABCA1 transcriptional activation, with ChIP confirming LXRα binding to the ABCA1 promoter upon AMPK activation, connecting energy-sensing to cholesterol efflux.

    Evidence AMPK siRNA/overexpression, LXRα siRNA epistasis, ChIP, pharmacological activators in human macrophages

    PMID:27343431

    Open questions at the time
    • How AMPK activates LXRα (direct phosphorylation vs indirect) not resolved
    • In vivo relevance in atherosclerosis models not tested
  17. 2017 High

    TRAK2 was identified as a negative regulator of LXR-mediated ABCA1 transcription, hepatocyte ABCA1 was shown essential for maintaining plasma membrane cholesterol and insulin signaling, and ABCA1-dependent microparticle release was quantified as ~30% of total cholesterol efflux.

    Evidence TRAK2 siRNA with ChIP showing increased LXR promoter binding; hepatocyte-specific ABCA1-KO with Akt phosphorylation and SREBP-1c readouts; inducible ABCA1 expression with MP characterization

    PMID:28129550 PMID:28591582 PMID:28655204

    Open questions at the time
    • Mechanism by which TRAK2 inhibits LXR promoter access unknown
    • Relative importance of MP release versus direct lipid transfer to apoA-I in vivo not established
  18. 2019 High

    ApoE4-driven ARF6 overexpression was shown to trap ABCA1 in late endosomes, impairing cholesterol efflux and Aβ degradation, and the CXCL12→CXCR4→GSK3β→β-catenin→TCF21 pathway was identified as a transcriptional repressor of ABCA1, while PMP22 was found to physically interact with ABCA1 and facilitate its plasma membrane localization in Schwann cells.

    Evidence ARF6 trafficking assays in ApoE4-TR mice; ChIP and reporter assays for TCF21/CXCL12 pathway with in vivo lentiviral overexpression; reciprocal co-IP from Schwann cell and nerve lysates with PMP22-KO and ABCA1-KO mice

    PMID:31061090 PMID:31641056 PMID:31662443

    Open questions at the time
    • Whether ARF6-mediated trapping is reversible therapeutically not established
    • TCF21 as ABCA1 activator demonstrated only in macrophages
    • PMP22–ABCA1 interaction domain not mapped
  19. 2020 Medium

    ABCA1 was linked to intraocular pressure regulation through modulation of caveolin-1/eNOS/NO signaling in Schlemm's canal endothelium, extending its functional repertoire beyond lipid metabolism.

    Evidence Lentiviral ABCA1 manipulation, transendothelial resistance, eNOS/NO measurement, in vivo intracameral GW3965 injection with IOP measurement

    PMID:32428234

    Open questions at the time
    • Single study without independent replication
    • Whether cholesterol efflux per se mediates eNOS derepression or a distinct ABCA1 mechanism operates is unclear
  20. 2025 Medium

    Caveolin-1 was identified by unbiased proteomics as a promoter of ABCA1 endolysosomal trapping, linking APOE4-associated ABCA1 mislocalization to oxysterol accumulation, mTORC1 activation, and cellular senescence.

    Evidence Discovery proteomics, ABCA1-KO cells and mice, APOE4-TR mice, iPSC-derived astrocytes, cyclodextrin rescue

    PMID:39901180

    Open questions at the time
    • Single recent study awaiting independent replication
    • Whether caveolin-1 directly binds ABCA1 or acts indirectly through membrane domains not determined
    • Therapeutic relevance of cyclodextrin in humans unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • A high-resolution structure of full-length ABCA1 in complex with apoA-I and/or lipid substrates is needed to explain the molecular basis of phospholipid flippase selectivity, cholesterol inhibition, and the coupling between ATP hydrolysis and lipid translocation.
  • No atomic-resolution structure of ABCA1 in lipid-bound or apoA-I-bound state
  • Direct lipid translocation has not been demonstrated with purified reconstituted protein
  • In vivo contributions of microparticle release versus direct apoA-I lipidation not quantified

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005215 transporter activity 5 GO:0008289 lipid binding 3 GO:0140657 ATP-dependent activity 2 GO:0060089 molecular transducer activity 1
Localization
GO:0005886 plasma membrane 6 GO:0005768 endosome 4 GO:0031410 cytoplasmic vesicle 2
Pathway
R-HSA-382551 Transport of small molecules 7 R-HSA-1430728 Metabolism 6 R-HSA-74160 Gene expression (Transcription) 5 R-HSA-5653656 Vesicle-mediated transport 4 R-HSA-162582 Signal Transduction 3 R-HSA-1643685 Disease 3 R-HSA-168256 Immune System 1
Partners
APOA1CAV1FLOT1OSBPPLD2PMP22STX13

Evidence

Reading pass · 34 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1999 ABC1/ABCA1 was identified as the gene defective in Tangier disease through combined gene expression microarray analysis, genetic mapping, and biochemical studies. Blocking ABC1 expression or activity reduces apolipoprotein-mediated lipid efflux from cultured cells, while increasing ABC1 expression enhances it. ABC1 protein is incorporated into the plasma membrane in proportion to its expression level. Gene expression microarray, genetic mapping, loss-of-function (siRNA/antibody), gain-of-function overexpression, plasma membrane fractionation The Journal of clinical investigation High 10431236 10431237 10431238 10525055
1999 Mutations in ABC1 were identified as the major cause of both Tangier disease and familial HDL deficiency associated with defective cholesterol efflux, establishing ABC1/ABCA1 as essential for HDL formation and the cholesterol efflux regulatory pathway. Genomic sequencing, restriction-fragment length polymorphism, cellular cholesterol efflux assay Lancet High 10533863 10535983
2000 ABCA1 expression drives specific binding of lipid-free apoA-I at the cell surface. Chemical cross-linking and immunoprecipitation showed apoA-I binds directly to ABCA1 protein. ABCA1 preferentially binds lipid-free apoA-I but not HDL. Cells overexpressing ABCA1 display plasma membrane protrusions (echinocyte-like morphology) consistent with excess lipids in the outer membrane leaflet, suggesting ABCA1 acts as a phospholipid/cholesterol flippase. Chemical cross-linking, co-immunoprecipitation, radiolabeled lipid efflux assay, immunofluorescence confocal microscopy, apoA-I binding assay The Journal of biological chemistry High 10918065
2000 ABC1 promotes Ca2+-induced exposure of phosphatidylserine at the plasma membrane outer leaflet and facilitates membrane microvesiculation and transbilayer redistribution of phospholipids. ABC1-deficient macrophages have impaired ability to engulf apoptotic cells, while ABC1 transfectants acquire phagocytic behavior, linking ABCA1-driven phosphatidylserine redistribution to apoptotic cell clearance. In vivo loss-of-function (ABC1-deficient mice), in vitro gain-of-function (transfection), prothrombinase assay, membrane microvesiculation assay, spin-labeled phospholipid transbilayer redistribution, phagocytosis assay Nature cell biology High 10878804
2000 LXRα/RXR and LXRβ/RXR heterodimers bind to a direct repeat spaced by four nucleotides (DR4) element at positions -70 to -55 bp in the ABCA1 promoter and drive sterol-dependent transactivation. Mutations in this DR4 element abolish sterol-mediated activation, establishing LXR/RXR as direct transcriptional regulators of ABCA1. Promoter-reporter transfection assay, site-directed mutagenesis of promoter, co-transfection with LXR/RXR expression vectors The Journal of biological chemistry High 10858438
2000 RXR heterodimers with oxysterol receptors (LXRs) regulate reverse cholesterol transport by controlling expression of the ABC1 transporter in peripheral tissues, while RXR/FXR heterodimers regulate bile acid synthesis. Treatment of animals with rexinoids markedly inhibited cholesterol absorption and repressed bile acid synthesis through these pathways. Receptor-selective agonist pharmacology in mice, tissue cholesterol balance measurements, gene expression analysis Science High 10968783
2002 cAMP/PKA-dependent pathway phosphorylates ABCA1, with maximum phosphorylation reached within 10 minutes representing a 260% increase over basal levels. PKA inhibitor H-89 reduced cholesterol efflux by 70% and almost completely inhibited ABCA1 phosphorylation. Tangier disease (homozygous ABCA1-deficient) cells showed no increase in ABCA1 phosphorylation or efflux upon cAMP stimulation, whereas familial HDL deficiency heterozygotes retained partial response. Radiolabeled phosphorylation assay, cholesterol efflux assay, PKA inhibitor (H-89), patient fibroblasts (Tangier disease and familial HDL deficiency) Journal of lipid research High 12454270
2003 RARγ/RXR directly activates the ABCA1 promoter through the same DR4 element used by LXR/RXR. Chromatin immunoprecipitation in macrophages confirmed binding of RARγ/RXR to the ABCA1 promoter DR4 element in the presence of all-trans retinoic acid. In RARγ-knockout macrophages, marked upregulation of RARα compensated and still induced ABCA1. Promoter-reporter transfection, chromatin immunoprecipitation (ChIP), RARγ knockout mouse macrophages, dose-response with retinoic acid receptor agonists Molecular and cellular biology High 14560020
2004 Probucol inhibits ABCA1-mediated cholesterol efflux by impairing translocation of ABCA1 from intracellular compartments to the plasma membrane. Probucol also inhibits formation of an ABCA1-linked cholesterol oxidase-sensitive plasma membrane domain and reduces cell-surface apoA-I binding. This effect was absent in Tangier patient fibroblasts lacking functional ABCA1, confirming specificity. Fluorescent confocal microscopy, biotinylation surface assay, cholesterol oxidase-sensitive domain assay, radiolabeled apoA-I binding assay, Tangier fibroblast controls Arteriosclerosis, thrombosis, and vascular biology High 15514211
2004 ABCA1 directly associates with syntaxin 13 (but not syntaxins 3 or 6) and flotillin-1, as demonstrated by immunoprecipitation. This complex resides in Lubrol WX-insoluble lipid raft microdomains and in phagosomes. siRNA silencing of syntaxin 13 reduced ABCA1 protein levels and significantly decreased apoA-I-dependent phospholipid efflux. Tangier fibroblasts with mutated ABCA1 showed enhanced phagocytosis that was normalized by re-expression of functional ABCA1. Co-immunoprecipitation, siRNA knockdown, immunofluorescence microscopy, detergent-resistant membrane fractionation, phagocytosis assay, lipid efflux assay Molecular biology of the cell High 15469992
2005 Unsaturated fatty acids destabilize ABCA1 through a phospholipase D2 (PLD2) signaling pathway. Long-chain acyl-CoA synthetase inhibitor triacsin C reversed this effect. PLD2 siRNA abolished unsaturated fatty acid-induced reduction in ABCA1 levels, increased serine phosphorylation of ABCA1, and inhibition of lipid transport activity. Diacylglycerol analogs also increased ABCA1 serine phosphorylation, implicating PLD2-generated diacylglycerols in destabilizing ABCA1 phosphorylation. siRNA knockdown of PLD2, pharmacological inhibitors (triacsin C, 1-butanol), phosphorylation assay, ABCA1 protein stability assay, lipid efflux assay The Journal of biological chemistry High 16118212
2006 Purified human ABCA1 reconstituted into liposomes exhibits robust ATPase activity. ATPase activity is stimulated preferentially by phospholipids with choline head groups (phosphatidylcholine and sphingomyelin) and inhibited by addition of cholesterol (25% decrease at 20% cholesterol). Beta-sitosterol and campesterol showed similar inhibitory effects but stigmasterol did not, indicating structure-specific sterol interactions. Glibenclamide suppressed ABCA1 ATPase activity, providing a biochemical basis for its inhibition of cholesterol efflux. Protein purification from Sf9 insect cells, liposome reconstitution, ATPase activity assay, trypsin limited-digestion conformational analysis The Journal of biological chemistry High 16500904
2007 ABCA1 mediates the mechanism of nascent HDL formation: binding of a small pool of apoA-I to ABCA1 enhances net phospholipid translocation to the plasma membrane exofacial leaflet, creating membrane strain relieved by formation of exovesiculated lipid domains. ApoA-I then binds at high affinity to these curved membrane domains and spontaneously solubilizes them to create discoidal nascent HDL particles containing 2–4 apoA-I molecules. Cell-based lipid efflux assay, apoA-I binding kinetics, analysis of HDL particle composition and size, phospholipid translocation assay The Journal of biological chemistry High 17604270
2007 Beta-cell-specific inactivation of Abca1 in mice causes markedly impaired glucose tolerance and defective insulin secretion with normal insulin sensitivity. Isolated islets from these mice show altered cholesterol homeostasis and impaired insulin secretion in vitro. Rosiglitazone (a PPARγ activator that upregulates Abca1 in beta-cells) requires beta-cell Abca1 for its beneficial effects on glucose tolerance, establishing a role for ABCA1 in beta-cell cholesterol homeostasis and insulin secretion. Conditional beta-cell-specific knockout mice, glucose tolerance tests, insulin secretion assay, ex vivo islet cholesterol measurement Nature medicine High 17322896
2008 ABCA1 and apoA-I undergo endocytosis via a clathrin- and Rab5-mediated pathway and are recycled back to the cell surface via a Rab4-mediated route. Approximately 30% of endocytosed ABCA1 is recycled to the cell surface. When receptor-mediated endocytosis is inhibited, apoA-I internalization is blocked and cholesterol efflux from cells with excess lipoprotein-derived cholesterol decreases, while efflux from cells without excess cholesterol increases, indicating that retroendocytosis contributes to HDL formation particularly when excess cholesterol has accumulated. Extracellular HA-tagged and intracellular GFP-tagged ABCA1 constructs, clathrin inhibition, dominant-negative Rab protein expression, cholesterol efflux assay Genes to cells High 19170766
2008 OSBP (oxysterol-binding protein) negatively regulates ABCA1 protein stability. OSBP knockdown increased ABCA1 protein half-life by 3-fold without affecting ABCA1 mRNA or LXR transcriptional activity. A mutation in the OSBP sterol-binding domain (but not mutations abrogating Golgi or ER interactions) prevented OSBP-mediated reduction of ABCA1, indicating that OSBP destabilizes ABCA1 via its sterol-binding domain. siRNA knockdown, ABCA1 protein half-life (cycloheximide chase), LXR reporter assay, OSBP domain mutants, cholesterol efflux assay The Journal of biological chemistry High 18450749
2009 LRP1 deficiency leads to increased PDGFRβ signaling and sequential MAPK activation, which increases phosphorylation of cytosolic phospholipase A2 (cPLA2). Activated cPLA2 releases arachidonic acid, which suppresses LXR/RXR heterodimer activation at the ABCA1 promoter, resulting in greatly reduced ABCA1 expression. Thus LRP1 regulates ABCA1 expression and reverse cholesterol transport by controlling cPLA2 activation. LRP1 knockout cell lines, signaling pathway inhibitors, cPLA2 phosphorylation assay, ABCA1 promoter reporter, cholesterol efflux assay PloS one Medium 19718435
2009 ABCA1 and ABCG1 modulate macrophage inflammatory cytokine and chemokine expression by controlling sterol efflux and cellular membrane sterol levels. Transporter deficiency causes increased signaling via Toll-like receptors including TLR4, mechanistically linking cholesterol efflux activity to anti-inflammatory and immunosuppressive functions. ABCA1/ABCG1 knockout macrophages, cytokine/chemokine expression assays, TLR signaling pathway analysis Arteriosclerosis, thrombosis, and vascular biology Medium 19797709
2011 ABCA1 protein is degraded by calpain in early endosomes after endocytosis. When HDL biogenesis is ongoing (helical apolipoproteins interacting with ABCA1), ABCA1 becomes resistant to calpain and is recycled to the cell surface. Factors that interact with ABCA1 (α1-syntrophin, LXRβ, calmodulin) retard this calpain-mediated degradation. Pharmacological inhibition of calpain increases ABCA1 activity and HDL biogenesis in vitro and in vivo. Calpain inhibitors, subcellular fractionation, ABCA1 protein stability assay, endocytosis tracking, in vivo pharmacology Biochimica et biophysica acta High 21835264
2011 ApoA-I/ABCA1 interaction activates multiple downstream signaling pathways including JAK2/STAT3, protein kinase A, Rho GTPase CDC42, and protein kinase C. PKA and CDC42 activation regulates ABCA1-mediated lipid efflux, PKC activation stabilizes ABCA1 protein, and JAK2/STAT3 activation regulates both lipid efflux and anti-inflammatory effects, demonstrating that ABCA1 functions both as a lipid transporter and a signaling receptor. Kinase inhibitors, dominant-negative constructs, phosphorylation assays, cholesterol efflux assay, cytokine production assay Biochimica et biophysica acta Medium 21920460
2012 miR-33a overexpression in human and mouse islets reduces ABCA1 expression, decreases glucose-stimulated insulin secretion, and increases cholesterol levels. The miR-33a-induced reduction in insulin secretion was rescued by cholesterol depletion (methyl-β-cyclodextrin or mevastatin), confirming that miR-33a suppresses insulin secretion through ABCA1-dependent islet cholesterol accumulation. Adenoviral miR-33a overexpression, miR-33a inhibition in ApoE-KO islets, ABCA1 overexpression rescue, cholesterol measurement, glucose-stimulated insulin secretion assay Diabetes High 22315319
2015 Apoptotic cells trigger a BAI1→ELMO1→Rac1 signaling pathway that rapidly upregulates ABCA1 mRNA and protein, independently of the LXR sterol-sensing pathway. Primary macrophages from BAI1-, ELMO1-, or Rac1-deficient mice showed attenuated ABCA1 induction. BAI1 overexpression increased ABCA1 induction in response to apoptotic cells, and BAI1-deficient mice on high-fat diet had increased apoptotic cells in aortic roots with altered lipid profiles. Primary macrophages from BAI1/ELMO1/Rac1 KO mice, ABCA1 mRNA/protein induction assay, transgenic BAI1 overexpression, high-fat diet in vivo model The Journal of clinical investigation High 26075824
2016 AMPK activation (pharmacological or genetic) increases ABCA1 and LXRα mRNA and protein in human macrophages, resulting in increased cholesterol efflux. Silencing LXRα (but not LXRβ) attenuated ABCA1 expression after AMPK activation. ChIP showed LXRα binding to the LXR responsive element in the ABCA1 promoter upon AMPK activation, placing AMPK upstream of LXRα in the regulatory pathway. AMPK siRNA/overexpression, pharmacological AMPK activators, LXRα siRNA, luciferase reporter assay, ChIP, cholesterol efflux assay The international journal of biochemistry & cell biology High 27343431
2017 TRAK2 knockdown significantly increases cholesterol efflux and ABCA1 mRNA and protein expression. TRAK2 effect on cholesterol efflux was abolished in the absence of ABCA1, confirming ABCA1 dependency. ChIP showed that TRAK2 knockdown increases LXR binding at the ABCA1 promoter, establishing TRAK2 as a novel negative regulator of LXR-mediated ABCA1 transcription. siRNA knockdown (TRAK2 and ABCA1), ChIP, cholesterol efflux assay, mRNA/protein expression, human genetic-transcriptomic data correlation European heart journal High 28655204
2017 Hepatocyte-specific ABCA1 knockout reduces plasma membrane free cholesterol content and impairs trafficking of lysosomal free cholesterol to the plasma membrane. This leads to reduced insulin-stimulated Akt phosphorylation, reduced SREBP-1c activation, and suppressed lipogenic gene expression, demonstrating that ABCA1 maintains hepatocyte plasma membrane cholesterol for efficient insulin signaling and lipogenesis. Hepatocyte-specific ABCA1 knockout mice, plasma membrane cholesterol fractionation, insulin signaling assays (Akt phosphorylation), SREBP-1c activation, glucose/insulin tolerance tests Cell reports High 28591582
2019 ApoE4 promotes greater expression of ARF6 compared with ApoE3, trapping ABCA1 in late endosomes and impairing its recycling to the cell membrane. This results in lower ABCA1-mediated cholesterol efflux, a greater percentage of lipid-free ApoE particles, and lower Aβ degradation capacity. Enhancing ABCA1 activity rescued impaired Aβ degradation in ApoE4-treated cells and reduced ApoE and ABCA1 aggregation in the hippocampus of ApoE4 knock-in mice. ARF6 expression analysis, ABCA1 trafficking assay (late endosome trapping), cholesterol efflux assay, ApoE lipidation assay, Aβ degradation assay, in vivo ApoE4-targeted replacement mice The Journal of neuroscience High 31641056
2019 PMP22 (peripheral myelin protein 22) physically interacts with ABCA1 at the Schwann cell plasma membrane (demonstrated by co-immunoprecipitation). Absence of PMP22 results in reduced ABCA1 localization to the cell membrane and diminished apoE secretion and ABCA1-mediated cholesterol efflux, despite upregulation of ABCA1 and apoE mRNA/protein. Conversely, ABCA1 knockout elevates PMP22 expression and aberrant processing. Co-immunoprecipitation from Schwann cell and nerve lysates, immunofluorescence co-localization, ABCA1 KO mice, PMP22 KO mice, cholesterol efflux assay, whole-cell patch clamp The Journal of neuroscience High 31061090
2006 ABCA1 deficiency in the brain of ABCA1 knockout mice causes a considerable decrease in soluble brain apoE protein levels and increases amyloid deposition in APP23 transgenic mice, including increased parenchymal and vascular (cerebral amyloid angiopathy) amyloid. The results establish ABCA1 as a regulator of apoE lipidation/solubility in brain and a modifier of amyloid pathology. ABCA1 knockout crossed with APP23 transgenic mice, Aβ immunoreactivity, thioflavine S staining, cerebral amyloid angiopathy assessment, apoE protein quantification (soluble vs insoluble fractions) The Journal of biological chemistry High 16207713
2006 HIV-1 Nef protein binds ABCA1 and impairs ABCA1-dependent cholesterol efflux from macrophages through two mechanisms: post-transcriptional downregulation of ABCA1 and redistribution of ABCA1 to the plasma membrane with inhibited apoA-I internalization. This causes lipid accumulation in HIV-infected macrophages resembling foam cells. Stimulation of cholesterol efflux reduced HIV virion infectivity by decreasing virion-associated cholesterol. Nef transfection in murine macrophages, ABCA1 protein expression analysis, ABCA1 trafficking (redistribution assay), co-immunoprecipitation (Nef-ABCA1 binding), cholesterol efflux assay, virion cholesterol measurement PLoS biology High 17076584
2009 HIF-1β availability determines ABCA1 expression and cholesterol efflux in macrophages under hypoxia. HIF-1 complex specifically binds to the HIF-1 response element in the ABCA1 promoter and increases ABCA1 promoter activity. ABCA1 expression and cholesterol efflux strongly correlated with HIF-1β expression in hypoxic macrophages and in human atherosclerotic lesion macrophages. HIF-1 response element binding assay, ABCA1 promoter-reporter assay, constitutively active HIF-1α overexpression, ChIP (inferred), cholesterol efflux under hypoxia, human atherosclerotic lesion analysis The international journal of biochemistry & cell biology Medium 19828131
2019 CXCL12 reduces ABCA1 expression and cholesterol efflux from macrophages via CXCR4→GSK3β phosphorylation→β-catenin Thr120 phosphorylation pathway. TCF21 directly stimulates ABCA1 transcription by binding to its promoter (confirmed by luciferase reporter and ChIP), and CXCL12 represses TCF21. Knockdown or inhibition of CXCR4 blocked CXCL12 effects on TCF21 and ABCA1 expression. Luciferase reporter assay, ChIP, siRNA knockdown (CXCR4), kinase inhibitors, protein phosphorylation assays, cholesterol efflux assay, in vivo lentiviral CXCL12 overexpression in Apoe-/- mice Journal of lipid research High 31662443
2020 ABCA1 regulates aqueous humor outflow and intraocular pressure via the caveolin-1/endothelial NO synthase (eNOS)/NO signaling pathway. ABCA1 upregulation in angular aqueous plexus (Schlemm's canal equivalent) cells decreases caveolin-1 expression and increases eNOS expression and NO production, reducing transendothelial resistance. In vivo intracameral injection of ABCA1 agonist GW3965 decreased IOP and increased conventional outflow facility. ABCA1 overexpression (lentivirus-ABCA1-shRNA or agonist GW3965), transendothelial electrical resistance measurement, eNOS/caveolin-1 protein expression, NO concentration measurement, in vivo intracameral injection, IOP measurement Investigative ophthalmology & visual science Medium 32428234
2017 ABCA1 mediates microparticle (MP) formation during HDL biogenesis. Using ABCA1 overexpression under inducible control and selective ABCA1 inactivation (probucol), MPs were shown to constitute approximately 30% of ABCA1-and apoA-I-mediated cholesterol efflux. Adding apoA-I markedly increases MP release. MPs contain flotillin-2 and CD63 but not apoA-I. Inducible ABCA1 expression system, probucol-mediated ABCA1 inhibition, methyl-β-cyclodextrin cholesterol depletion, MP characterization (size, markers), cholesterol efflux assay Atherosclerosis Medium 28129550
2025 Caveolin-1 acts as a key promoter of ABCA1 endolysosomal trafficking identified by discovery proteomics. In APOE4 and AD models, greater caveolin-1 expression traps ABCA1 in lysosomes, causing oxysterol accumulation, mTORC1 activation, and cellular senescence. Cyclodextrin treatment reduced brain oxysterol levels, ABCA1 lysosome trapping, mTORC1 activation, and senescence markers in APOE4-TR mice. Unbiased proteomics (discovery), ABCA1 knockout cell lines and mouse models, APOE4-TR mice, iPSC-derived astrocytes, mTORC1 phosphorylation assay, senescence markers, cyclodextrin treatment Molecular neurodegeneration Medium 39901180

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2010 Biological, clinical and population relevance of 95 loci for blood lipids. Nature 2873 20686565
2013 Discovery and refinement of loci associated with lipid levels. Nature genetics 2409 24097068
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
1999 Mutations in ABC1 in Tangier disease and familial high-density lipoprotein deficiency. Nature genetics 1430 10431236
2008 Newly identified loci that influence lipid concentrations and risk of coronary artery disease. Nature genetics 1310 18193043
1999 The gene encoding ATP-binding cassette transporter 1 is mutated in Tangier disease. Nature genetics 1292 10431237
1999 Tangier disease is caused by mutations in the gene encoding ATP-binding cassette transporter 1. Nature genetics 1209 10431238
2008 Six new loci associated with blood low-density lipoprotein cholesterol, high-density lipoprotein cholesterol or triglycerides in humans. Nature genetics 1130 18193044
2008 Common variants at 30 loci contribute to polygenic dyslipidemia. Nature genetics 1113 19060906
2000 Regulation of absorption and ABC1-mediated efflux of cholesterol by RXR heterodimers. Science (New York, N.Y.) 1101 10968783
2004 Multiple rare alleles contribute to low plasma levels of HDL cholesterol. Science (New York, N.Y.) 829 15297675
2000 Sterol-dependent transactivation of the ABC1 promoter by the liver X receptor/retinoid X receptor. The Journal of biological chemistry 828 10858438
2009 DNA methylation differences after exposure to prenatal famine are common and timing- and sex-specific. Human molecular genetics 766 19656776
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2008 Loci influencing lipid levels and coronary heart disease risk in 16 European population cohorts. Nature genetics 695 19060911
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2005 HDAC6 and microtubules are required for autophagic degradation of aggregated huntingtin. The Journal of biological chemistry 599 16192271
1999 The Tangier disease gene product ABC1 controls the cellular apolipoprotein-mediated lipid removal pathway. The Journal of clinical investigation 594 10525055
2009 Role of HDL, ABCA1, and ABCG1 transporters in cholesterol efflux and immune responses. Arteriosclerosis, thrombosis, and vascular biology 557 19797709
2008 Polymorphisms associated with cholesterol and risk of cardiovascular events. The New England journal of medicine 530 18354102
2000 Specific binding of ApoA-I, enhanced cholesterol efflux, and altered plasma membrane morphology in cells expressing ABC1. The Journal of biological chemistry 502 10918065
2000 ABC1 promotes engulfment of apoptotic cells and transbilayer redistribution of phosphatidylserine. Nature cell biology 437 10878804
2012 Genome-wide association study identifies multiple loci influencing human serum metabolite levels. Nature genetics 436 22286219
2010 Genetic variants near TIMP3 and high-density lipoprotein-associated loci influence susceptibility to age-related macular degeneration. Proceedings of the National Academy of Sciences of the United States of America 433 20385819
1999 Molecular cloning of the human ATP-binding cassette transporter 1 (hABC1): evidence for sterol-dependent regulation in macrophages. Biochemical and biophysical research communications 413 10092505
2008 Association of loss-of-function mutations in the ABCA1 gene with high-density lipoprotein cholesterol levels and risk of ischemic heart disease. JAMA 395 18523221
2010 Genome-wide association study of advanced age-related macular degeneration identifies a role of the hepatic lipase gene (LIPC). Proceedings of the National Academy of Sciences of the United States of America 376 20385826
2010 Common inherited variation in mitochondrial genes is not enriched for associations with type 2 diabetes or related glycemic traits. PLoS genetics 363 20714348
2010 Genetic variants influencing circulating lipid levels and risk of coronary artery disease. Arteriosclerosis, thrombosis, and vascular biology 351 20864672
2007 Beta-cell ABCA1 influences insulin secretion, glucose homeostasis and response to thiazolidinedione treatment. Nature medicine 346 17322896
2005 Lack of ABCA1 considerably decreases brain ApoE level and increases amyloid deposition in APP23 mice. The Journal of biological chemistry 312 16207713
2006 Role of apoA-I, ABCA1, LCAT, and SR-BI in the biogenesis of HDL. Journal of molecular medicine (Berlin, Germany) 297 16501936
1999 Mutations in the ABC1 gene in familial HDL deficiency with defective cholesterol efflux. Lancet (London, England) 289 10533863
2007 Mechanism of ATP-binding cassette transporter A1-mediated cellular lipid efflux to apolipoprotein A-I and formation of high density lipoprotein particles. The Journal of biological chemistry 274 17604270
2006 Human immunodeficiency virus impairs reverse cholesterol transport from macrophages. PLoS biology 274 17076584
2001 Apolipoprotein specificity for lipid efflux by the human ABCAI transporter. Biochemical and biophysical research communications 269 11162594
2011 Genome-wide association study of coronary heart disease and its risk factors in 8,090 African Americans: the NHLBI CARe Project. PLoS genetics 258 21347282
2000 Age and residual cholesterol efflux affect HDL cholesterol levels and coronary artery disease in ABCA1 heterozygotes. The Journal of clinical investigation 258 11086027
2015 HDL particle size is a critical determinant of ABCA1-mediated macrophage cellular cholesterol export. Circulation research 254 25589556
2000 ABCA1-mediated transport of cellular cholesterol and phospholipids to HDL apolipoproteins. Current opinion in lipidology 234 10882340
1999 Human ATP-binding cassette transporter 1 (ABC1): genomic organization and identification of the genetic defect in the original Tangier disease kindred. Proceedings of the National Academy of Sciences of the United States of America 216 10535983
2003 HDL apolipoproteins and ABCA1: partners in the removal of excess cellular cholesterol. Arteriosclerosis, thrombosis, and vascular biology 199 12615680
2000 Tangier disease and ABCA1. Biochimica et biophysica acta 183 11111099
2001 Structure, function and regulation of the ABC1 gene product. Current opinion in lipidology 174 11264984
2005 Transcriptional regulatory networks in lipid metabolism control ABCA1 expression. Biochimica et biophysica acta 172 15922656
2005 ABCA1 gene deletion protects against cerebral malaria: potential pathogenic role of microparticles in neuropathology. The American journal of pathology 145 15632021
2018 Is ABCA1 a lipid transfer protein? Journal of lipid research 139 29305383
2004 Probucol inhibits ABCA1-mediated cellular lipid efflux. Arteriosclerosis, thrombosis, and vascular biology 127 15514211
2021 The Role of the ATP-Binding Cassette A1 (ABCA1) in Human Disease. International journal of molecular sciences 125 33562440
2019 ApoE4 Alters ABCA1 Membrane Trafficking in Astrocytes. The Journal of neuroscience : the official journal of the Society for Neuroscience 120 31641056
2012 miR-33a modulates ABCA1 expression, cholesterol accumulation, and insulin secretion in pancreatic islets. Diabetes 119 22315319
2019 Quercetin protects against atherosclerosis by regulating the expression of PCSK9, CD36, PPARγ, LXRα and ABCA1. International journal of molecular medicine 118 31524223
2014 ABCA1 and nascent HDL biogenesis. BioFactors (Oxford, England) 117 25359426
2001 Regulation and intracellular trafficking of the ABCA1 transporter. Journal of lipid research 114 11518753
2020 Brothers in Arms: ABCA1- and ABCG1-Mediated Cholesterol Efflux as Promising Targets in Cardiovascular Disease Treatment. Pharmacological reviews 108 31831519
2003 Retinoic acid receptor-mediated induction of ABCA1 in macrophages. Molecular and cellular biology 108 14560020
2022 Molecular mechanisms for ABCA1-mediated cholesterol efflux. Cell cycle (Georgetown, Tex.) 102 35192423
2002 cAMP induces ABCA1 phosphorylation activity and promotes cholesterol efflux from fibroblasts. Journal of lipid research 102 12454270
2015 Apoptotic cells trigger a membrane-initiated pathway to increase ABCA1. The Journal of clinical investigation 101 26075824
2001 Localization of human ATP-binding cassette transporter 1 (ABC1) in normal and atherosclerotic tissues. Arteriosclerosis, thrombosis, and vascular biology 97 11231917
2016 ABCA1 Agonist Reverses the ApoE4-Driven Cognitive and Brain Pathologies. Journal of Alzheimer's disease : JAD 96 27567858
2009 The cell cholesterol exporter ABCA1 as a protector from cardiovascular disease and diabetes. Biochimica et biophysica acta 96 19344785
2012 The interaction of ApoA-I and ABCA1 triggers signal transduction pathways to mediate efflux of cellular lipids. Molecular medicine (Cambridge, Mass.) 92 22064972
2006 Purification and ATPase activity of human ABCA1. The Journal of biological chemistry 91 16500904
2003 Study of ABCA1 function in transgenic mice. Arteriosclerosis, thrombosis, and vascular biology 90 12615681
2005 Unsaturated fatty acids phosphorylate and destabilize ABCA1 through a phospholipase D2 pathway. The Journal of biological chemistry 86 16118212
2006 Tetrahymena Genome Database (TGD): a new genomic resource for Tetrahymena thermophila research. Nucleic acids research 82 16381920
2013 ABCA1 influences neuroinflammation and neuronal death. Neurobiology of disease 81 23376685
2005 ABCA1 and atherosclerosis. Vascular medicine (London, England) 78 16013195
2011 Regulation of ABCA1 functions by signaling pathways. Biochimica et biophysica acta 77 21920460
2007 Adiponectin deficiency suppresses ABCA1 expression and ApoA-I synthesis in the liver. FEBS letters 74 17936760
2004 Serum amyloid A promotes ABCA1-dependent and ABCA1-independent lipid efflux from cells. Biochemical and biophysical research communications 73 15358117
2001 Regulation and activity of the human ABCA1 gene in transgenic mice. The Journal of biological chemistry 73 11279093
2019 MiR‑30e and miR‑92a are related to atherosclerosis by targeting ABCA1. Molecular medicine reports 70 30816508
2003 Regulation of ABCA1 expression and cholesterol efflux during adipose differentiation of 3T3-L1 cells. Journal of lipid research 64 12754274
2020 Diabetes Impairs Cellular Cholesterol Efflux From ABCA1 to Small HDL Particles. Circulation research 62 32819213
2013 A chloroplast ABC1-like kinase regulates vitamin E metabolism in Arabidopsis. Plant physiology 62 23632854
2008 Retroendocytosis pathway of ABCA1/apoA-I contributes to HDL formation. Genes to cells : devoted to molecular & cellular mechanisms 62 19170766
2004 Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Molecular biology of the cell 61 15469992
2003 Identification and characterization of rodent ABCA1 in isolated type II pneumocytes. American journal of physiology. Lung cellular and molecular physiology 56 12909583
2011 Function and regulation of ABCA1--membrane meso-domain organization and reorganization. The FEBS journal 55 21554545
2005 Assembly of high density lipoprotein by the ABCA1/apolipoprotein pathway. Current opinion in lipidology 54 15891387
2020 Regulation of ATP binding cassette transporter A1 (ABCA1) expression: cholesterol-dependent and - independent signaling pathways with relevance to inflammatory lung disease. Respiratory research 53 32977800
2006 Regulation of ABCA1 expression in human keratinocytes and murine epidermis. Journal of lipid research 52 16825673
2019 CXCL12 promotes atherosclerosis by downregulating ABCA1 expression via the CXCR4/GSK3β/β-cateninT120/TCF21 pathway. Journal of lipid research 51 31662443
2008 Effects of fibrate drugs on expression of ABCA1 and HDL biogenesis in hepatocytes. Journal of cardiovascular pharmacology 49 18356690
2022 Role of ABCA1 in Cardiovascular Disease. Journal of personalized medicine 48 35743794
2020 The crosstalk of ABCA1 and ANXA1: a potential mechanism for protection against atherosclerosis. Molecular medicine (Cambridge, Mass.) 48 32894039
2017 ATP binding cassette A1 (ABCA1) mediates microparticle formation during high-density lipoprotein (HDL) biogenesis. Atherosclerosis 48 28129550
2015 Increased Systemic and Plaque Inflammation in ABCA1 Mutation Carriers With Attenuation by Statins. Arteriosclerosis, thrombosis, and vascular biology 47 26109739
2022 ABCA1, ABCG1, and Cholesterol Homeostasis. Advances in experimental medicine and biology 45 35575923
2008 OSBP negatively regulates ABCA1 protein stability. The Journal of biological chemistry 45 18450749
2020 ABCA1/ApoE/HDL Signaling Pathway Facilitates Myelination and Oligodendrogenesis after Stroke. International journal of molecular sciences 44 32575457
2019 PMP22 Regulates Cholesterol Trafficking and ABCA1-Mediated Cholesterol Efflux. The Journal of neuroscience : the official journal of the Society for Neuroscience 42 31061090
2018 Small molecule inducers of ABCA1 and apoE that act through indirect activation of the LXR pathway. Journal of lipid research 41 29563219
2017 Plasminogen promotes cholesterol efflux by the ABCA1 pathway. JCI insight 41 28768900
2016 AMPK activates LXRα and ABCA1 expression in human macrophages. The international journal of biochemistry & cell biology 41 27343431
2011 Calpain-mediated ABCA1 degradation: post-translational regulation of ABCA1 for HDL biogenesis. Biochimica et biophysica acta 40 21835264
2020 ABCA1/ABCB1 Ratio Determines Chemo- and Immune-Sensitivity in Human Osteosarcoma. Cells 38 32155954
2010 ABCA1 gene variants regulate postprandial lipid metabolism in healthy men. Arteriosclerosis, thrombosis, and vascular biology 38 20185793
2016 ABCA1/ApoE/HDL Pathway Mediates GW3965-Induced Neurorestoration After Stroke. Stroke 37 28028143
2009 LRP1 controls cPLA2 phosphorylation, ABCA1 expression and cellular cholesterol export. PloS one 37 19718435
2019 MiR-200b-3p Functions as an Oncogene by Targeting ABCA1 in Lung Adenocarcinoma. Technology in cancer research & treatment 36 31795847
2011 Hepatic ABCA1 and VLDL triglyceride production. Biochimica et biophysica acta 36 22001232
2007 Expression and biological activity of ABCA1 in alveolar epithelial cells. American journal of respiratory cell and molecular biology 36 17884990
2016 Piperine inhibits ABCA1 degradation and promotes cholesterol efflux from THP-1-derived macrophages. Molecular nutrition & food research 35 27862930
2015 IRAK1 mediates TLR4-induced ABCA1 downregulation and lipid accumulation in VSMCs. Cell death & disease 34 26512959
2012 Hesperetin upregulates ABCA1 expression and promotes cholesterol efflux from THP-1 macrophages. Journal of natural products 34 22429094
2002 ABCA1: regulation, trafficking and association with heteromeric proteins. Annals of medicine 34 12452478
2014 Identification of microRNAs 758 and 33b as potential modulators of ABCA1 expression in human atherosclerotic plaques. Nutrition, metabolism, and cardiovascular diseases : NMCD 33 25445880
2007 ABCA1 expression in humans is associated with physical activity and alcohol consumption. Atherosclerosis 33 17481640
2020 Molecular mechanism for nobiletin to enhance ABCA1/G1 expression in mouse macrophages. Atherosclerosis 32 32062137
2017 Hepatocyte ABCA1 Deletion Impairs Liver Insulin Signaling and Lipogenesis. Cell reports 32 28591582
2017 TRAK2, a novel regulator of ABCA1 expression, cholesterol efflux and HDL biogenesis. European heart journal 30 28655204
2001 Human and mouse ABCA1 comparative sequencing and transgenesis studies revealing novel regulatory sequences. Genomics 30 11352567
1996 Cloning by functional complementation, and inactivation, of the Schizosaccharomyces pombe homologue of the Saccharomyces cerevisiae gene ABC1. Molecular & general genetics : MGG 30 8668131
2019 Impact of natural products on the cholesterol transporter ABCA1. Journal of ethnopharmacology 29 31805338
2012 Myeloid cell-specific ABCA1 deletion protects mice from bacterial infection. Circulation research 29 22955730
2023 QiShenYiQi pill inhibits atherosclerosis by promoting reverse cholesterol transport PPARγ-LXRα/β-ABCA1 pathway. Journal of ethnopharmacology 28 37230281
2022 Cholesterol homeostasis regulated by ABCA1 is critical for retinal ganglion cell survival. Science China. Life sciences 28 35829808
2021 Recent advances in the regulation of ABCA1 and ABCG1 by lncRNAs. Clinica chimica acta; international journal of clinical chemistry 28 33545111
2009 HIF-1beta determines ABCA1 expression under hypoxia in human macrophages. The international journal of biochemistry & cell biology 28 19828131
2004 A tRNA(TRP) gene mediates the suppression of cbs2-223 previously attributed to ABC1/COQ8. Biochemical and biophysical research communications 28 15063807
2022 HDL, cholesterol efflux, and ABCA1: Free from good and evil dualism. Journal of pharmacological sciences 27 36055755
2013 AtSIA1 AND AtOSA1: two Abc1 proteins involved in oxidative stress responses and iron distribution within chloroplasts. The New phytologist 27 24117441
2004 Oxysterols trigger ABCA1-mediated basolateral surfactant efflux. American journal of respiratory cell and molecular biology 27 15039140
2022 Roles of ABCA1 in cancer. Oncology letters 26 36072007
2021 Geniposide Ameliorated Dexamethasone-Induced Cholesterol Accumulation in Osteoblasts by Mediating the GLP-1R/ABCA1 Axis. Cells 26 34943934
2025 Cellular senescence induced by cholesterol accumulation is mediated by lysosomal ABCA1 in APOE4 and AD. Molecular neurodegeneration 25 39901180
2020 ABCA1 Regulates IOP by Modulating Cav1/eNOS/NO Signaling Pathway. Investigative ophthalmology & visual science 25 32428234