Affinage

ZNF143

Zinc finger protein 143 · UniProt P52747

Length
638 aa
Mass
68.9 kDa
Annotated
2026-06-11
100 papers in source corpus 32 papers cited in narrative 32 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ZNF143 (the human ortholog of Xenopus Staf) is a sequence-specific C2H2 zinc-finger transcription factor that directly activates both protein-coding (Pol II) and small nuclear/tRNA-type (Pol II and Pol III) genes across thousands of mammalian promoters (PMID:7641696, PMID:9009278, PMID:9705341, PMID:17092945). It recognizes its target motif through a zinc-finger array in which fingers 3–6 form the high-affinity DNA-binding core, with peripheral fingers selectively deployed for different sites, and it drives transcription through two physically and functionally distinct activation domains — one selective for mRNA promoters and an Oct-1-dependent activity at snRNA promoters (PMID:9566884, PMID:10446199, PMID:10455183). Mechanistically, ZNF143 facilitates RNA polymerase II initiation at nearly all activated target genes, and at a subset of loci it represses transcription by competing with stronger activators and physically occluding promoter-proximal elements (PMID:39676670); it can activate Pol III transcription from chromatin templates, associating with the chromatin remodeler CHD8 at the U6 promoter (PMID:17938208). ZNF143 forms a mutually dependent complex with THAP11 and HCFC1 at active CpG-island promoters, where it recruits HCF-1 to control cell-cycle gene expression and proliferation (PMID:23539139, PMID:25437553, PMID:26416877), and it cooperates with partners including p73 to activate DNA-repair genes and LIN28B to drive oncogenic transcriptional programs (PMID:17297437, PMID:32601179). Its target genes encompass metabolic, cell-cycle, and developmental regulators — including transaldolase, BUB1B, TFAM, Skp2, CEBPA, and the developmental gene pax2a — and ZNF143 is essential for normal vertebrate development (PMID:14702349, PMID:17478512, PMID:17707600, PMID:21352097, PMID:22268977, PMID:28900037). Although ZNF143 co-occupies CTCF/cohesin sites and was implicated in chromatin looping (PMID:25645053, PMID:30120652, PMID:33397967), acute-degradation experiments established that it has no general looping function — prior associations were confounded by antibody cross-reactivity with CTCF — and showed instead that ZNF143 is an essential promoter-proximal transcriptional regulator with unusually stable chromatin binding (dwell time >20 min) that safeguards expression of nuclear-encoded mitochondrial genes (PMID:39708805, PMID:39708803). Mutations in ZNF143 cause an inborn error of cobalamin metabolism through loss of MMACHC regulation (PMID:27349184).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 1995 High

    Established the founding activity by showing the protein is a DNA-binding activator of an RNA Pol III gene, defining ZNF143/Staf as a transcription factor rather than a generic zinc-finger protein.

    Evidence Cloning and in vitro binding plus Xenopus oocyte microinjection of the selenocysteine tRNA promoter

    PMID:7641696

    Open questions at the time
    • Restricted to a single tRNA target
    • No mammalian validation yet
    • Activation domain architecture undefined
  2. 1997 High

    Generalized the activator role across the snRNA gene class transcribed by both Pol II and Pol III, defining a consensus binding site and showing it is necessary for activation.

    Evidence DNA-binding assays and oocyte microinjection of mutant snRNA-type genes

    PMID:9009278

    Open questions at the time
    • Did not address protein-coding promoters
    • Human ortholog not yet established
  3. 1998 High

    Resolved how a single factor activates distinct promoter classes by mapping two separable activation domains, and confirmed human ZNF143 is the functional Staf ortholog.

    Evidence Deletion/point mutagenesis with in vivo transcription in oocytes and Drosophila cells; gel shift and chimeric-protein assays

    PMID:9566884 PMID:9705341

    Open questions at the time
    • Coactivators bridging activation domains to the machinery unknown
    • Promoter selectivity mechanism of each domain unresolved
  4. 2000 High

    Defined the DNA-recognition logic by mapping the zinc-finger array, showing fingers 3–6 form the high-affinity core and peripheral fingers are deployed flexibly per target.

    Evidence Incremental zinc-finger truncations, EMSA, missing-nucleoside interference, and a model based on solved analogous structures

    PMID:10446199 PMID:10455183 PMID:10773080

    Open questions at the time
    • No crystal structure of the ZNF143–DNA complex
    • Structural basis of Oct-1 cooperativity inferred, not solved
  5. 2007 High

    Expanded the target repertoire to specific protein-coding genes and revealed partner-dependent functions in DNA repair and cell-cycle control.

    Evidence ChIP, EMSA, reporter mutagenesis on transaldolase, BUB1B, TFAM; co-IP with p73 and knockdown/drug-sensitivity assays; in vitro chromatin transcription and CHD8 interactome

    PMID:14702349 PMID:17297437 PMID:17478512 PMID:17707600 PMID:17938208

    Open questions at the time
    • Whether p73 stimulation is direct or via cofactors not fully resolved
    • CHD8 recruitment mechanism to ZNF143 sites unclear
  6. 2013 High

    Placed ZNF143 in a defined nuclear protein complex by showing co-occupancy with HCFC1 at thousands of CpG-island promoters and motif-driven recruitment of THAP11/HCF-1.

    Evidence ChIP-seq co-occupancy with HCFC1 and motif analysis; reciprocal co-IP, ChIP, and proliferation/cell-cycle knockdown assays; CRISPR editing of the ACTACA submotif

    PMID:23539139 PMID:25437553 PMID:26416877

    Open questions at the time
    • Stoichiometry and assembly order of the THAP11/ZNF143/HCFC1 complex
    • How the complex modifies the transcription machinery
  7. 2015 High

    Linked ZNF143 occupancy to three-dimensional genome organization, reporting preferential binding at chromatin-interaction anchors and CTCF/cohesin co-binding.

    Evidence Integration of ChIP-seq with Hi-C, ZNF143 silencing, SNP and motif analyses across human and mouse genomes

    PMID:25645053 PMID:30120652 PMID:33397967

    Open questions at the time
    • Knockdown/deletion approaches could not separate looping cause from transcriptional consequence
    • Antibody specificity not yet questioned
  8. 2016 Medium

    Connected ZNF143 to human disease by identifying causative mutations in an inborn error of cobalamin metabolism acting through loss of MMACHC regulation.

    Evidence Whole-exome sequencing, BioID confirmation of HCFC1 interaction, and qRT-PCR of MMACHC in patient and siRNA-treated fibroblasts

    PMID:27349184

    Open questions at the time
    • Whether the two mutations act via the same molecular defect not dissected
    • Broader phenotypic spectrum of ZNF143 deficiency unknown
  9. 2020 Medium

    Extended ZNF143 into oncogenic transcriptional programs, showing it activates MDIG/CDC6 in hepatocellular carcinoma and serves as a chromatin-docking partner for LIN28B in neuroblastoma.

    Evidence ChIP, reporter and knockdown assays with xenografts; ChIP-seq and co-IP with let-7-binding-deficient LIN28B mutant

    PMID:32312832 PMID:32601179

    Open questions at the time
    • Direct versus indirect contribution of ZNF143 to LIN28B target activation
    • Generality of these programs beyond the studied cancers
  10. 2024 High

    Overturned the looping model using acute degradation and antibody validation, establishing ZNF143 as a stably bound promoter-proximal transcription factor that safeguards nuclear-encoded mitochondrial genes rather than mediating loops.

    Evidence Auxin-inducible degron in mouse and human cells, Hi-C, GRO-seq/RNA-seq, FRAP dwell-time imaging, and demonstration of antibody cross-reactivity with CTCF; combinatorial dual-degron of CTCF and ZNF143

    PMID:38206813 PMID:39708803 PMID:39708805

    Open questions at the time
    • How extremely stable promoter dwell time is achieved mechanistically
    • Residual ZNF143 contribution to CTCF/cohesin geometry remains debated across 2024 reports
  11. 2025 High

    Defined the molecular mode of action by showing ZNF143 facilitates Pol II initiation at activated genes and represses others by occluding promoter-proximal elements as a polymerase roadblock.

    Evidence Rapid degron depletion with nascent RNA sequencing (GRO-seq/PRO-seq) and ChIP-seq analysis of promoter occupancy

    PMID:39676670

    Open questions at the time
    • Identity of the general initiation factors ZNF143 recruits not pinned down
    • Determinants distinguishing activated from repressed targets unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unknown how ZNF143's two activation domains and its THAP11/HCFC1 complex mechanistically engage the Pol II/III initiation machinery to produce its activating versus repressive outcomes.
  • No structure of ZNF143 bound to promoter DNA or to the THAP11/HCFC1 complex
  • Direct biochemical link between ZNF143 and basal initiation factors undefined
  • Mechanism of its unusually stable chromatin dwell time unresolved

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 5 GO:0003677 DNA binding 4 GO:0140223 general transcription initiation factor activity 1
Localization
GO:0005634 nucleus 3 GO:0000228 nuclear chromosome 2
Pathway
R-HSA-74160 Gene expression (Transcription) 5 R-HSA-1640170 Cell Cycle 4 R-HSA-1266738 Developmental Biology 2
Partners
CHD8CTCFHCFC1LIN28BOCT-1P73THAP11
Complex memberships
THAP11/ZNF143/HCFC1 complex

Evidence

Reading pass · 32 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1995 Staf (ZNF143 Xenopus ortholog) was cloned and shown to bind a 15 bp activator element in the selenocysteine tRNA gene promoter and activate RNA polymerase III transcription in vivo; the protein contains seven zinc fingers and a separate acidic activation domain. Cloning, in vitro binding assays, expression assay in Xenopus oocytes by microinjection The EMBO journal High 7641696
1997 Staf (ZNF143 ortholog) is required for enhanced transcription of the majority of vertebrate snRNA and snRNA-type genes transcribed by both RNA polymerase II and III; a 19 bp consensus Staf-binding site was derived and shown necessary for activation in vivo. DNA binding assays, microinjection of mutant genes into Xenopus oocytes, recombinant Staf functional assays The EMBO journal High 9009278
1998 Staf contains two physically and functionally distinct activation domains outside the DNA-binding domain: a 93-amino-acid domain with four repeated units selective for mRNA promoters, and an 18-amino-acid domain (with critical Leu-213) selective for Pol II and Pol III snRNA promoters. In vivo transcription assays with Staf deletion/point mutants in Xenopus oocytes and Drosophila cells Molecular and cellular biology High 9566884
1998 Human ZNF143 is 84% identical to Xenopus Staf and constitutes its human ortholog; ZNF143 (and ZNF76) bind the Staf DNA motif with similar affinity, activate transcription from mRNA and snRNA promoters in Drosophila cells and Xenopus oocytes through the Staf binding site, and chimeric proteins carrying a heterologous DNA-binding domain activate Pol II and Pol III promoters. Gel shift assays, transfection in Drosophila cells, microinjection in Xenopus oocytes using chimeric proteins The Journal of biological chemistry High 9705341
1999 Staf zinc fingers 3–6 constitute the minimal region for high-affinity DNA binding; zinc finger 7 makes no base-specific contacts; zinc finger 1 is required for binding to the Xenopus tRNA(Sec) site but dispensable for the human U6 site, demonstrating flexible utilization of zinc fingers for different target sequences. Binding site selection, EMSA with truncated Staf zinc finger polypeptides, interference (missing nucleoside) experiments The Journal of biological chemistry High 10446199
1999 Optimal transcriptional activation of the human U6 gene requires zinc fingers 2–7 of Staf plus Oct-1 co-binding, whereas the Xenopus tRNA(Sec) gene needs all seven zinc fingers but not Oct-1; insertion of a zinc finger 1 binding site into the U6 promoter increased Staf binding but interfered with simultaneous Oct-1 binding and reduced transcription. Xenopus oocyte microinjection, EMSA, missing nucleoside interference with wild-type and mutant promoters The Journal of biological chemistry High 10455183
2000 Staf zinc fingers 1–6 make extensive DNA major groove contacts, predominantly with the non-template strand; zinc fingers 3–6 form the high-affinity core; a structural model of Staf–DNA complexes was proposed based on biochemical mapping and analogy to solved zinc finger–DNA structures. Binding assays with incremental zinc finger truncations, interference experiments, binding site selection Nucleic acids research Medium 10773080
2003 ZNF143 drives basal and tissue-specific expression of the human transaldolase gene by binding the core promoter (nt −29 to −16); dominant-negative ZNF143 DNA-binding domain abolished promoter activity; overexpression of ZNF143 increased transaldolase enzyme activity; ChIP confirmed in vivo occupancy. DNase I footprinting, EMSA, reporter assays with mutant promoters, overexpression/dominant-negative transfection, ChIP, enzyme activity assay The Journal of biological chemistry High 14702349
2004 STAF/ZNF143 (murine ortholog) binds the aldehyde reductase promoter 5′ element and drives constitutive expression; CHOP competes with STAF for the same binding site and mediates stress-induced induction in the human but not mouse promoter. Gel-shift assays, ChIP, deletion/mutation reporter analysis, transfection Genomics Medium 14667815
2006 A genome-scale in silico and biochemical analysis identified ~1175 conserved ZNF143 binding sites in ~938 mammalian promoters; ChIP confirmed 90% are bona fide ZNF143 targets; the presence of a single ZNF143 binding site is sufficient to direct luciferase reporter expression, suggesting ZNF143 can independently recruit transcription machinery. Bioinformatics binding-site identification, ChIP across 295 promoters, luciferase reporter assays The Journal of biological chemistry Medium 17092945
2007 ZNF143 interacts with tumor suppressor p73 (but not p53); p73 stimulates ZNF143 binding to its recognition site and to cisplatin-modified DNA; ZNF143 directly activates Rad51 and FEN1 transcription; ZNF143 knockdown sensitizes prostate cancer cells to cisplatin. Co-immunoprecipitation, ChIP, siRNA knockdown, drug sensitivity assays Oncogene Medium 17297437
2007 ZNF143 (hStaf) is required for transcription of the human BUB1B (BubR1) gene; two conserved ZNF143 binding sites in the BUB1B promoter are indispensable for promoter activity; ZNF143 occupancy on the BUB1B promoter was confirmed by ChIP. EMSA, mutant reporter transfection assays, ChIP Nucleic acids research Medium 17478512
2007 ZNF143 (hStaf) is required for expression of the human TFAM gene; two conserved ZNF143 binding sites were identified by promoter binding assays; mutant reporter assays and ChIP confirmed functional occupancy. Promoter binding assays, mutant TFAM reporter transfection, ChIP Gene Medium 17707600
2007 ZNF143 activates U6 snRNA transcription from a preassembled chromatin template in vitro and associates with chromatin-modifying proteins including CHD8; CHD8 binds histone H3 dimethylated and trimethylated on K4, resides on the U6 promoter in vivo, and contributes to efficient Pol III transcription. In vitro chromatin transcription assay, mass spectrometry interactome of Staf/ZNF143, ChIP Molecular and cellular biology High 17938208
2010 ZNF143 controls expression of divergent (bidirectional) gene pairs; ZNF143 binding sites are overrepresented in bidirectional versus unidirectional promoters; ChIP confirmed 93% occupancy; dual reporter assays showed dependence on ZNF143 binding site integrity; ZNF143 per se exhibits inherent bidirectional transcription activity. In silico binding-site analysis, ChIP, dual luciferase bidirectional reporter assays Nucleic acids research Medium 21177654
2011 STAF/ZNF143 binds two conserved sites in the Skp2 promoter (identified by EMSA and ChIP) and is necessary and sufficient for Skp2 promoter activity and adhesion-dependent Skp2 expression; siRNA knockdown of STAF reduces Skp2 mRNA and protein and inhibits proliferation; ectopic Skp2 fully rescues STAF-silencing growth inhibition. EMSA, ChIP, promoter-reporter transfection, siRNA knockdown, rescue with ectopic Skp2 The Biochemical journal High 21352097
2012 ZNF143 transcriptional activator is essential for normal development in zebrafish; morpholino knockdown causes pleiotropic defects (heart, blood, ear, midbrain-hindbrain boundary); rescue requires the amino-terminal activation domains; the pax2a promoter contains two ZNF143 binding sites and is directly activated by ZNF143. Morpholino knockdown in zebrafish, mRNA rescue with wild-type vs. activation-domain-deleted ZNF143, reporter assays with pax2a promoter BMC molecular biology High 22268977
2012 ZNF143 promotes GPX1 activity and protects mitochondrial respiratory-deficient cells from oxidative stress; ZNF143 and GPX1 double knockdown showed that ZNF143 upregulates GPX1 activity in the context of mitochondrial dysfunction; ZNF143 also activates the selenocysteine synthesis pathway (SepSecS gene expression) under these conditions. ZNF143 and GPX1 siRNA knockdown, GPX enzyme activity assays, GSH measurement, gene expression analysis Cell death & disease Medium 23152058
2013 ZNF143 co-localizes with HCFC1 at ~5400 active CpG-island promoters in HeLa cells; the DNA sequences underlying HCFC1 binding sites contain ZNF143 (and THAP11) recognition motifs; ~90% of HCFC1-bound promoters are co-occupied by ZNF143. ChIP-seq for HCFC1 and ZNF143, motif analysis, genomic co-occupancy analysis Genome research Medium 23539139
2014 THAP11 and ZNF143 form a mutually dependent complex with HCFC1 on chromatin; HCF-1 recruitment to E2F-bound promoters is mediated by THAP11 and ZNF143, not E2F directly; disruption of the THAP11/ZNF143/HCF-1 complex reduces expression of cell-cycle control genes, cell proliferation, cell-cycle progression, and cell viability. Co-immunoprecipitation, ChIP, siRNA knockdown, cell proliferation and cell-cycle assays Cell reports High 25437553
2015 ZNF143 preferentially occupies anchors of chromatin interactions connecting promoters with distal regulatory elements; silencing ZNF143 disrupts chromatin interactions at gene promoters; SNP-mediated alteration of ZNF143 DNA-binding affinity reduces chromatin interactions in a sequence-dependent manner; chromatin interactions alone do not regulate gene expression. Integration of ChIP-seq with Hi-C/chromatin interaction maps (ENCODE), ZNF143 silencing, SNP analysis as surrogate mutagenesis Nature communications Medium 25645053
2015 The ACTACA submotif, shared by THAP11 and ZNF143, directs recruitment of THAP11 and HCFC1 to ZNF143-occupied loci; position, spacing, and orientation of this submotif relative to the ZNF143 core motif are critical; CRISPR-Cas9 alteration of ACTACA at endogenous promoters altered gene transcription and histone modifications. Chromosomally integrated synthetic constructs, CRISPR-Cas9 endogenous promoter editing, ChIP, gene expression analysis Molecular and cellular biology High 26416877
2016 Mutations in ZNF143 (p.L284* and p.T340I) cause an inborn error of cobalamin metabolism; ZNF143 interacts with HCFC1 (confirmed by proximity biotinylation); ZNF143 regulates expression of the cobalamin trafficking gene MMACHC, as shown by reduced MMACHC expression in patient fibroblasts and in control fibroblasts treated with ZNF143 siRNA. Whole-exome sequencing, proximity biotinylation (BioID) for ZNF143–HCFC1 interaction, qRT-PCR of MMACHC in patient and siRNA-treated cells Human mutation Medium 27349184
2017 ZNF143 binds a conserved CCCAGCAG octameric sequence ~100 bp upstream of the CEBPA transcription start site and activates C/EBPα expression in myeloid cells; mutational analysis showed this 8-bp sequence is crucial for C/EBPα expression; the mechanism in myeloid cells is distinct from that in liver and adipocytes. Mutational analysis of CEBPA promoter, reporter assays, ChIP, siRNA knockdown The Journal of biological chemistry Medium 28900037
2018 ZNF143 co-binds with CTCF-Cohesin at chromatin loop anchors and acts as a cofactor of the CTCF-Cohesin complex; siRNA knockdown of ZNF143 in HEK293T cells followed by in situ Hi-C showed that the majority of chromatin loops are lost or weakened after ZNF143 silencing. siRNA knockdown, in situ Hi-C, computational motif analysis, aggregate peak analysis Cell biology and toxicology Medium 30120652
2020 ZNF143 directly activates transcription of MDIG histone demethylase, which reduces H3K9me3 at the CDC6 promoter, thereby activating CDC6 expression and promoting hepatocellular carcinoma cell-cycle progression; ZNF143 knockdown reduces CDC6 and MDIG expression and tumor growth. ChIP, reporter assays, siRNA/shRNA knockdown, xenograft assays, co-expression analysis Cancer research Medium 32312832
2020 LIN28B binds active gene promoters in neuroblastoma cells through protein-protein interaction with ZNF143 (shown by ChIP-seq and co-immunoprecipitation) and activates downstream targets including adrenergic core regulatory circuitry transcription factors, GSK3B, and L1CAM; this is a let-7-independent transcriptional function. ChIP-seq, co-immunoprecipitation, overexpression of wild-type vs. let-7-binding-deficient LIN28B mutant Proceedings of the National Academy of Sciences of the United States of America Medium 32601179
2021 ZNF143 is a key regulator of CTCF-bound promoter-enhancer loops in the murine genome; ZNF143 and CTCF binding motifs are distributed ~37 bp apart in convergent orientation at many genomic sites; genetic deletion of ZNF143 causes loss of CTCF binding at promoters and enhancers and disrupts promoter-enhancer loops essential for hematopoietic stem and progenitor cell function. ChIP-seq, Hi-C/chromatin interaction mapping, ZNF143 genetic deletion in mouse, hematopoietic cell functional assays Nature communications High 33397967
2024 Acute degradation of ZNF143/ZFP143 using an auxin-inducible degron in mouse and human cells shows no general chromatin looping function; a commonly used ZNF143 antibody cross-reacts with CTCF, explaining prior reports linking ZNF143 to loops; ZNF143 specifically activates nuclear-encoded mitochondrial genes and its loss causes severe mitochondrial dysfunction; ZNF143 binds promoters with an extremely stable chromatin dwell time (>20 min) and functions largely independently of CTCF. Auxin-inducible degron protein degradation, Hi-C/chromatin interaction mapping, GRO-seq/RNA-seq, FRAP/live imaging for dwell time, antibody cross-reactivity validation Molecular cell High 39708805
2024 Contrary to prior reports, ZNF143/ZFP143 possesses no general chromatin looping function; it is an essential transcription factor that binds promoters proximally with extremely stable dwell time (>20 min), regulates a subset of mitochondrial and ribosomal genes, and functions largely independently of CTCF. Dual degron/imaging tags on CTCF and ZNF143, combinatorial acute degradation, Hi-C, live-cell imaging, RNA-seq Molecular cell High 39708803
2024 ZNF143 deletion alters CTCF/cohesin geometry at numerous CTCF sites; ZNF143 is located between CTCF and cohesin and its removal narrows the space between them; ZNF143 and CTCF collaborate in higher-order TAD organization; CTCF depletion enlarges direct ZNF143-mediated chromatin looping at promoter-enhancer sites. ChIP-seq, Hi-C, genetic deletion of ZNF143, acute CTCF degradation (combinatorial), cohesin ChIP Cell reports Medium 38206813
2025 ZNF143 facilitates RNA polymerase II initiation at target genes; ZNF143 binds promoters of nearly all activated target genes; ZNF143 also directly represses a subset of genes by competing with more efficient activators for promoter access, physically occluding transcription initiation sites and promoter-proximal elements, and acting as a molecular roadblock to RNA polymerases during early elongation. Rapid ZNF143 degradation (degron), nascent RNA sequencing (GRO-seq/PRO-seq), ChIP-seq, mechanistic analysis of promoter occupancy Nucleic acids research High 39676670

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 Genomic binding sites of the yeast cell-cycle transcription factors SBF and MBF. Nature 816 11206552
2004 Cln3 activates G1-specific transcription via phosphorylation of the SBF bound repressor Whi5. Cell 318 15210110
1997 SBF cell cycle regulator as a target of the yeast PKC-MAP kinase pathway. Science (New York, N.Y.) 220 9065400
2015 ZNF143 provides sequence specificity to secure chromatin interactions at gene promoters. Nature communications 148 25645053
2009 Linking cell cycle to histone modifications: SBF and H2B monoubiquitination machinery and cell-cycle regulation of H3K79 dimethylation. Molecular cell 146 19682934
2001 Clinical Trials Update: CAPRICORN, COPERNICUS, MIRACLE, STAF, RITZ-2, RECOVER and RENAISSANCE and cachexia and cholesterol in heart failure. Highlights of the Scientific Sessions of the American College of Cardiology, 2001. European journal of heart failure 140 11378012
1996 Switching transcription on and off during the yeast cell cycle: Cln/Cdc28 kinases activate bound transcription factor SBF (Swi4/Swi6) at start, whereas Clb/Cdc28 kinases displace it from the promoter in G2. Genes & development 139 8566747
2001 Cdk1 triggers association of RNA polymerase to cell cycle promoters only after recruitment of the mediator by SBF. Molecular cell 119 11430824
2009 Development of a biomimetic collagen-hydroxyapatite scaffold for bone tissue engineering using a SBF immersion technique. Journal of biomedical materials research. Part B, Applied biomaterials 117 19180526
1997 Staf, a promiscuous activator for enhanced transcription by RNA polymerases II and III. The EMBO journal 92 9009278
1995 A yeast transcription factor bypassing the requirement for SBF and DSC1/MBF in budding yeast has homology to bacterial signal transduction proteins. The EMBO journal 92 8521825
1997 Role of the casein kinase I isoform, Hrr25, and the cell cycle-regulatory transcription factor, SBF, in the transcriptional response to DNA damage in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences of the United States of America 90 9012827
2013 HCFC1 is a common component of active human CpG-island promoters and coincides with ZNF143, THAP11, YY1, and GABP transcription factor occupancy. Genome research 84 23539139
1991 Silencer region of a chalcone synthase promoter contains multiple binding sites for a factor, SBF-1, closely related to GT-1. Plant molecular biology 81 1893099
1998 ZNF76 and ZNF143 are two human homologs of the transcriptional activator Staf. The Journal of biological chemistry 78 9705341
2020 Assembly of the Thiolated [Au1 Ag22 (S-Adm)12 ]3+ Superatom Complex into a Framework Material through Direct Linkage by SbF6 - Anions. Angewandte Chemie (International ed. in English) 75 32003526
1995 Staf, a novel zinc finger protein that activates the RNA polymerase III promoter of the selenocysteine tRNA gene. The EMBO journal 73 7641696
2009 The E2F functional analogue SBF recruits the Rpd3(L) HDAC, via Whi5 and Stb1, and the FACT chromatin reorganizer, to yeast G1 cyclin promoters. The EMBO journal 64 19745812
2012 Sbf/MTMR13 coordinates PI(3)P and Rab21 regulation in endocytic control of cellular remodeling. Molecular biology of the cell 61 22648168
2021 ZNF143 mediates CTCF-bound promoter-enhancer loops required for murine hematopoietic stem and progenitor cell function. Nature communications 58 33397967
2007 CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription. Molecular and cellular biology 55 17938208
2007 ZNF143 interacts with p73 and is involved in cisplatin resistance through the transcriptional regulation of DNA repair genes. Oncogene 52 17297437
2006 A genome scale location analysis of human Staf/ZNF143-binding sites suggests a widespread role for human Staf/ZNF143 in mammalian promoters. The Journal of biological chemistry 52 17092945
2020 ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma. Cancer research 48 32312832
2010 Role of ZNF143 in tumor growth through transcriptional regulation of DNA replication and cell-cycle-associated genes. Cancer science 48 20860770
2014 Host cell factor-1 recruitment to E2F-bound and cell-cycle-control genes is mediated by THAP11 and ZNF143. Cell reports 47 25437553
2007 Transcription of the human cell cycle regulated BUB1B gene requires hStaf/ZNF143. Nucleic acids research 46 17478512
2001 X-ray crystal structures of alpha-KrF(2),[KrF][MF(6)](M=As,Sb,Bi),[Kr(2)F(3)][SbF(6).KrF(2), [Ke(2)F(3)2[SbF(6)]2.KrF(2), and [Kr(2)F(3)][AsF(6)].[KrF][AsF(6)]; synthesis and characterization of [Kr(2)F(3)][PF(6).nKrF(2); and theoretical studies of KrF(2), KrF+, Kr(2)F(3)+, and the [KrF][MF(6)](M=P,As,Sb,Bi) ion pairs. Inorganic chemistry 44 11399167
2020 LIN28B regulates transcription and potentiates MYCN-induced neuroblastoma through binding to ZNF143 at target gene promotors. Proceedings of the National Academy of Sciences of the United States of America 39 32601179
2016 ZNF143 enhances metastasis of gastric cancer by promoting the process of EMT through PI3K/AKT signaling pathway. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 39 27449034
1998 Two distinct domains in Staf to selectively activate small nuclear RNA-type and mRNA promoters. Molecular and cellular biology 38 9566884
2014 SBF-1 exerts strong anticervical cancer effect through inducing endoplasmic reticulum stress-associated cell death via targeting sarco/endoplasmic reticulum Ca(2+)-ATPase 2. Cell death & disease 35 25522275
2003 ZNF143 mediates basal and tissue-specific expression of human transaldolase. The Journal of biological chemistry 35 14702349
2014 The combination of strong expression of ZNF143 and high MIB-1 labelling index independently predicts shorter disease-specific survival in lung adenocarcinoma. British journal of cancer 34 24736586
2010 Genome-wide evidence for an essential role of the human Staf/ZNF143 transcription factor in bidirectional transcription. Nucleic acids research 34 21177654
2020 ZNF143 in Chromatin Looping and Gene Regulation. Frontiers in genetics 32 32318100
1999 Flexible zinc finger requirement for binding of the transcriptional activator staf to U6 small nuclear RNA and tRNA(Sec) promoters. The Journal of biological chemistry 31 10446199
2022 BUB1B (BUB1 Mitotic Checkpoint Serine/Threonine Kinase B) promotes lung adenocarcinoma by interacting with Zinc Finger Protein ZNF143 and regulating glycolysis. Bioengineered 30 35068350
2018 SBF assays, direct and indirect cell culture tests to evaluate the biological performance of bioglasses and bioglass-based composites: Three paradigmatic cases. Materials science & engineering. C, Materials for biological applications 30 30606588
2012 The transcriptional activator ZNF143 is essential for normal development in zebrafish. BMC molecular biology 30 22268977
2012 ZNF143 transcription factor mediates cell survival through upregulation of the GPX1 activity in the mitochondrial respiratory dysfunction. Cell death & disease 29 23152058
2004 Regulation of aldehyde reductase expression by STAF and CHOP. Genomics 29 14667815
2018 ZNF143 is a regulator of chromatin loop. Cell biology and toxicology 28 30120652
2016 Inborn Error of Cobalamin Metabolism Associated with the Intracellular Accumulation of Transcobalamin-Bound Cobalamin and Mutations in ZNF143, Which Codes for a Transcriptional Activator. Human mutation 28 27349184
2014 Synthesis, cytotoxicity, and hydroxyapatite formation in 27-Tris-SBF for sol-gel based CaO-P2O5-SiO2-B2O3-ZnO bioactive glasses. Scientific reports 26 24637634
2007 The SBF- and MBF-associated protein Msa1 is required for proper timing of G1-specific transcription in Saccharomyces cerevisiae. The Journal of biological chemistry 26 18160399
1999 Maximization of selenocysteine tRNA and U6 small nuclear RNA transcriptional activation achieved by flexible utilization of a Staf zinc finger. The Journal of biological chemistry 23 10455183
2019 Cell ratcheting through the Sbf RabGEF directs force balancing and stepped apical constriction. The Journal of cell biology 22 31562231
2015 Genomic Determinants of THAP11/ZNF143/HCFC1 Complex Recruitment to Chromatin. Molecular and cellular biology 21 26416877
2010 Transcriptional reporters for genes activated by cell wall stress through a non-catalytic mechanism involving Mpk1 and SBF. Yeast (Chichester, England) 21 20641022
2005 Homoleptic, sigma-bonded octahedral [M(CO)(6)](2+) cations of iron(II), ruthenium(II), and osmium(II). Part 1: Syntheses, thermochemical and vibrational characterizations, and molecular structures as [Sb(2)F(11)](-) and [SbF(6)](-) salts. A comprehensive, comparative study. Inorganic chemistry 21 15934748
2024 Putative looping factor ZNF143/ZFP143 is an essential transcriptional regulator with no looping function. Molecular cell 20 39708803
2022 Human hepatocyte-enriched miRNA-192-3p promotes HBV replication through inhibiting Akt/mTOR signalling by targeting ZNF143 in hepatic cell lines. Emerging microbes & infections 20 35109781
2019 Inhibition of the aberrant A1CF-FAM224A-miR-590-3p-ZNF143 positive feedback loop attenuated malignant biological behaviors of glioma cells. Journal of experimental & clinical cancer research : CR 20 31186064
2017 YPC-21661 and YPC-22026, novel small molecules, inhibit ZNF143 activity in vitro and in vivo. Cancer science 20 28192620
2016 Effect of apatite formation of biphasic calcium phosphate ceramic (BCP) on osteoblastogenesis using simulated body fluid (SBF) with or without bovine serum albumin (BSA). Materials science & engineering. C, Materials for biological applications 20 27772726
2013 Snf1/AMPK promotes SBF and MBF-dependent transcription in budding yeast. Biochimica et biophysica acta 20 24084603
2015 Blockade of the interaction between Bcr-Abl and PTB1B by small molecule SBF-1 to overcome imatinib-resistance of chronic myeloid leukemia cells. Cancer letters 19 26721204
2013 Biomineralization of hydroxyapatite on DNA molecules in SBF: morphological features and computer simulation. Langmuir : the ACS journal of surfaces and colloids 19 23980633
2012 SBF-1, a synthetic steroidal glycoside, inhibits melanoma growth and metastasis through blocking interaction between PDK1 and AKT3. Biochemical pharmacology 19 22525724
2012 Calcium phosphate-mediated gene delivery using simulated body fluid (SBF). International journal of pharmaceutics 19 22664458
1998 Molecular cloning and characterization of the murine staf cDNA encoding a transcription activating factor for the selenocysteine tRNA gene in mouse mammary gland. The Journal of biological chemistry 19 9535833
1986 Suppression of a pokeweed mitogen-stimulated plaque-forming cell response by a human B lymphocyte-derived aggregated IgG-stimulated suppressor factor: suppressive B cell factor (SBF). Journal of immunology (Baltimore, Md. : 1950) 19 2419423
2011 Adhesion-dependent Skp2 transcription requires selenocysteine tRNA gene transcription-activating factor (STAF). The Biochemical journal 18 21352097
2007 Transcription factor hStaf/ZNF143 is required for expression of the human TFAM gene. Gene 18 17707600
2006 Cell cycle-dependent regulation of Saccharomyces cerevisiae donor preference during mating-type switching by SBF (Swi4/Swi6) and Fkh1. Molecular and cellular biology 18 16809780
2020 ZNF143 Suppresses Cell Apoptosis and Promotes Proliferation in Gastric Cancer via ROS/p53 Axis. Disease markers 17 32076462
2017 ZNF143 protein is an important regulator of the myeloid transcription factor C/EBPα. The Journal of biological chemistry 17 28900037
2011 Forced Expression of ZNF143 Restrains Cancer Cell Growth. Cancers 17 24213117
1983 Suppressive B-cell factor (SBF) produced by FcR-bearing B cells; suppression of B, but not non-B-cell proliferation. Immunology 17 6350167
1981 Biologic and molecular characterization of the IgG serum blocking factor (SBF-IgG) isolated from sera of patients with EBV-induced infectious mononucleosis. Journal of immunology (Baltimore, Md. : 1950) 17 6263976
2024 ZNF143 deletion alters enhancer/promoter looping and CTCF/cohesin geometry. Cell reports 16 38206813
2021 3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM. Scientific reports 16 33649401
2024 ZNF143 is a transcriptional regulator of nuclear-encoded mitochondrial genes that acts independently of looping and CTCF. Molecular cell 15 39708805
2020 Solution combustion synthesis (SCS) of theranostic ions doped biphasic calcium phosphates; kinetic of ions release in simulated body fluid (SBF) and reactive oxygen species (ROS) generation. Materials science & engineering. C, Materials for biological applications 15 33255086
2019 The Role of ZNF143 in Breast Cancer Cell Survival Through the NAD(P)H Quinone Dehydrogenase 1⁻p53⁻Beclin1 Axis Under Metabolic Stress. Cells 15 30935019
2018 Fabrication and biological properties of calcium phosphate/chitosan composite coating on titanium in modified SBF. Materials science & engineering. C, Materials for biological applications 14 29853074
2016 [ZNF143 is involved in CTCF-mediated chromatin interactions by cooperation with cohesin and other partners]. Molekuliarnaia biologiia 14 27414788
2000 Structural organization of Staf-DNA complexes. Nucleic acids research 14 10773080
2023 ZNF143 inhibits hepatocyte mitophagy and promotes non-alcoholic fatty liver disease by targeting increased lncRNA NEAT1 expression to activate ROCK2 pathway. Epigenetics 13 37566742
2019 Expression of zinc finger transcription factors (ZNF143 and ZNF281) in serous borderline ovarian tumors and low-grade ovarian cancers. Journal of ovarian research 13 30885238
2022 The role of ZNF143 overexpression in rat liver cell proliferation. BMC genomics 12 35780101
2022 FBXO9 Mediates the Cancer-Promoting Effects of ZNF143 by Degrading FBXW7 and Facilitates Drug Resistance in Hepatocellular Carcinoma. Frontiers in oncology 12 35847937
2011 Hydrolysis of monetite/chitosan composites in α-MEM and SBF solutions. Journal of materials science. Materials in medicine 12 21479638
2008 A novel genetic screen implicates Elm1 in the inactivation of the yeast transcription factor SBF. PloS one 12 18231587
2020 Two paralogous znf143 genes in zebrafish encode transcriptional activator proteins with similar functions but expressed at different levels during early development. BMC molecular and cell biology 11 31969120
2020 Bioactivity in SBF versus trace element effects: The isolated role of Mg2+ and Zn2+ in osteoblast behavior. Materials science & engineering. C, Materials for biological applications 11 33254959
2023 ZNF143 facilitates the growth and migration of glioma cells by regulating KPNA2-mediated Hippo signalling. Scientific reports 10 37423952
2020 The ubiquitous transcriptional protein ZNF143 activates a diversity of genes while assisting to organize chromatin structure. Gene 10 33031894
2022 ZNF143 Expression is Associated with COPD and Tumor Microenvironment in Non-Small Cell Lung Cancer. International journal of chronic obstructive pulmonary disease 9 35400998
2012 Yeast IME2 functions early in meiosis upstream of cell cycle-regulated SBF and MBF targets. PloS one 9 22393365
2025 ZNF143 binds DNA and stimulates transcription initiation to activate and repress direct target genes. Nucleic acids research 8 39676670
2021 Selective targeting of the androgen receptor-DNA binding domain by the novel antiandrogen SBF-1 and inhibition of the growth of prostate cancer cells. Investigational new drugs 8 33411211
2018 SBF-1 preferentially inhibits growth of highly malignant human liposarcoma cells. Journal of pharmacological sciences 8 30415825
2010 The protonation of HSO(3)F: preparation and characterization of fluorodihydroxyoxosulfonium hexafluoroantimonate [H(2)SO(3)F](+)[SbF(6)](-). Chemistry (Weinheim an der Bergstrasse, Germany) 8 19943282
1986 Decreased suppressive B cell factor (SBF) in rheumatoid arthritis: evidence for a defect in B cell autoregulation. Journal of immunology (Baltimore, Md. : 1950) 8 3485142
1983 Monoclonal SBF produced by a hybridoma: in-vitro and in-vivo suppression of B tumour-cell proliferation. Immunology 8 6360850
2024 CXXC5 drove inflammation and ovarian cancer proliferation via transcriptional activation of ZNF143 and EGR1. Cellular signalling 7 38642782
2023 ATF1 promotes the malignancy of lung adenocarcinoma cells by transcriptionally regulating ZNF143 expression. Acta biochimica et biophysica Sinica 7 37158648
1998 On the Reaction of Phosphorous Acid with Superacids and the Crystal Structure of H(5)O(2)(+)SbF(6)(-) and Me(4)N(+)HPF(5)(-). Inorganic chemistry 7 11670618

Missed literature

Know a paper Affinage missed for ZNF143? Flag it for the maintainers and the community.

No submissions yet.