Affinage

HCFC1

Host cell factor 1 · UniProt P51610

Length
2035 aa
Mass
208.7 kDa
Annotated
2026-06-10
100 papers in source corpus 50 papers cited in narrative 50 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

HCFC1 (HCF-1) is a chromatin-associated transcriptional co-regulator that couples sequence-specific DNA-binding factors to histone-modifying machinery to control cell proliferation, cell-cycle progression, and developmental gene programs (PMID:12670868, PMID:12743030). It is synthesized as a precursor that undergoes site-specific proteolytic maturation by O-GlcNAc transferase (OGT) at centrally located HCF-1PRO repeats, generating an HCF-1N and an HCF-1C subunit that remain associated through interdigitated fibronectin type-3 (SAS1/SAS2) elements and a C-terminal nuclear localization signal (PMID:21295698, PMID:10958670, PMID:23045687). Structural and biochemical work established that OGT engages the HCF-1PRO repeat via its TPR domain, positions the cleavage region in the glycosyltransferase active site, and cleaves between cysteine and glutamate to yield a pyroglutamate product, with glycosylation and proteolysis being mechanistically separable activities of the same active site (PMID:24311690, PMID:27056667). The two subunits perform distinct cell-cycle roles: HCF-1N promotes G1/S progression, recruiting MLL/Set1 H3K4 methyltransferases and the Sin3 HDAC complex to target promoters together with sequence-specific factors such as E2F1, while HCF-1C ensures proper mitotic exit and cytokinesis, in part by regulating the H4-K20 methyltransferase PR-Set7 and mitotic histone modification status (PMID:12670868, PMID:12743030, PMID:15200950, PMID:17612494). HCF-1 is targeted to thousands of active promoters by a small set of partner factors — notably THAP11, ZNF143, and E2F family proteins binding via an HCF-1 binding motif (HBM) and shared submotifs — to direct transcription of cell-cycle, biosynthetic, and metabolic genes (PMID:20581084, PMID:23539139, PMID:26416877), including direct activation of CDC42 (PMID:33097698). Its activity is gated by post-translational and metabolic inputs: glucose-stimulated O-GlcNAcylation licenses binding to ChREBP for epigenetic activation of lipogenic genes (PMID:31227231), HSP90 maintains nuclear HCF-1 stability and expression of its cell-cycle target genes (PMID:31693902), and BAP1 deubiquitinates Lys-48 chains on the HCF-1N Kelch domain to modulate proliferation (PMID:19815555). In vivo, HCF-1 is required for cell-cycle re-entry and proliferation in regenerating liver and for epiblast survival and gastrulation (PMID:26921005, PMID:27521049), and it promotes herpesvirus reactivation by driving removal of repressive heterochromatin from latent viral genomes (PMID:36692302). Missense mutations in the HCFC1 Kelch domain cause X-linked cblX disorder, linked to loss of HCFC1-driven MMACHC transcription (PMID:24011988).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 2000 High

    Established the structural logic of subunit association, answering how the two HCF-1 fragments remain a functional unit after processing.

    Evidence Domain deletion, co-IP, and nuclear localization assays defining SAS1/SAS2 and the C-terminal NLS

    PMID:10958670

    Open questions at the time
    • Did not resolve how processing is triggered
    • Atomic structure of the interface not yet defined at this stage
  2. 2001 High

    Showed that HCF-1 chromatin association through its Kelch/beta-propeller domain is required for proliferation, linking a binding activity to a proliferative function.

    Evidence Chromatin fractionation and temperature-shift in tsBN67 cells

    PMID:11340173

    Open questions at the time
    • Did not identify the chromatin-tethering partner factors
    • Mechanism connecting chromatin loss to arrest unresolved
  3. 2002 Medium

    Connected HCF-1 to multiple regulatory contexts — spliceosomal snRNPs, a C-terminal activation domain augmented by p300, the pRb pathway, and HPIP-mediated CRM1 export — broadening its functional reach beyond a single coactivator role.

    Evidence Co-IP, in vitro splicing, reporter assays, genetic epistasis, and CRM1-inhibition localization studies

    PMID:12149646 PMID:12215534 PMID:12235138 PMID:12271126 PMID:12456665

    Open questions at the time
    • Splicing role not integrated with transcriptional functions
    • Physiological significance of cytoplasmic export incompletely defined
  4. 2003 High

    Defined the two-subunit, two-cell-cycle-stage model and the histone-modifier tethering function, establishing HCF-1 as a bifunctional cell-cycle regulator.

    Evidence siRNA depletion across cell lines with cell-cycle/cytokinesis readouts, co-IP and domain mapping of Sin3 and Set1/Ash2 complexes, and HBM analysis across DNA-binding proteins

    PMID:12670868 PMID:12743030 PMID:14532282

    Open questions at the time
    • Did not explain how proteolysis separates the two functions mechanistically
    • Promoter-level target genes not yet genome-wide
  5. 2004 High

    Identified PR-Set7/H4-K20 methylation control as the molecular basis of HCF-1C mitotic function, linking the C-subunit to chromosome segregation.

    Evidence siRNA depletion, histone modification Westerns, and PR-Set7 overexpression rescue

    PMID:15200950

    Open questions at the time
    • Mechanism by which HCF-1C controls PR-Set7 expression unresolved
  6. 2007 High

    Mapped HCF-1's G1/S coactivator mechanism onto E2F factors and conserved mitotic H3S10 phosphorylation control, defining its proliferative gene-activation route.

    Evidence Co-IP, ChIP at E2F promoters, cell-cycle staging, and C. elegans/mammalian H3S10P analysis

    PMID:17612494 PMID:18043729

    Open questions at the time
    • How HCF-1 switches between E2F activator and repressor complexes not fully defined
  7. 2008 High

    Revealed a conserved repressive role for HCF-1 on FOXO/DAF-16 signaling and lifespan, extending its function to stress and longevity regulation.

    Evidence C. elegans co-IP, ChIP, genetic epistasis, and lifespan assays; extended with SIR-2.1 epistasis and conservation in mammals

    PMID:18828672 PMID:21909281

    Open questions at the time
    • Direct chromatin mechanism of DAF-16 repression not fully resolved
    • Mammalian longevity relevance only inferred from target-gene overlap
  8. 2010 High

    Established HCF-1's promoter-targeting partners (THAP1, THAP11/Ronin) and its direct participation in HSV DNA replication and Golgi-gated localization, integrating recruitment and viral life-cycle control.

    Evidence Proteomics, in vitro binding, co-IP, ChIP, ChIP-seq in ESCs, immunofluorescence in neurons, and Asf1b depletion with viral DNA readouts

    PMID:18667495 PMID:20133788 PMID:20200153 PMID:20581084

    Open questions at the time
    • Signal triggering Golgi-to-nucleus relocalization undefined
    • How a single factor coordinates host transcription and viral replication unclear
  9. 2011 High

    Resolved that OGT is the protease that cleaves HCF-1 and that cleavage per se, not merely fragment separation, is required for HCF-1C function, unifying glycosylation and maturation under one enzyme.

    Evidence In vitro OGT cleavage assays, HCF-1PRO mutagenesis, and cell-based M-phase assays

    PMID:21295698

    Open questions at the time
    • Structural basis of cleavage not yet determined at this stage
  10. 2013 High

    Provided the structural and genome-wide mechanism: how OGT positions and cleaves the PRO repeat, and how a few sequence-specific factors direct HCFC1 to thousands of active promoters, plus the disease link to cblX.

    Evidence Crystal structure of OGT:HCF-1PRO complex with active-site mutagenesis; ChIP-seq with motif/co-localization analysis; patient mutation and MMACHC promoter studies

    PMID:23539139 PMID:24011988 PMID:24311690

    Open questions at the time
    • Whether all co-localizing factors bind HCFC1 directly not validated
    • How cblX mutations alter chromatin engagement not structurally resolved
  11. 2016 High

    Demonstrated in vivo requirement for HCF-1 in proliferation and development and dissected OGT's dual glycosylation/proteolysis activities into separable mechanisms.

    Evidence Conditional knockout in mouse hepatocytes/epiblast; active-site mutagenesis and engineered single-activity OGT variants

    PMID:26921005 PMID:27056667 PMID:27521049

    Open questions at the time
    • Direct transcriptional targets driving embryonic/regenerative phenotypes not fully mapped
  12. 2019 High

    Linked HCF-1 to nutrient-responsive metabolic transcription and to HSP90-dependent stability, showing how its activity and abundance are gated by upstream signals.

    Evidence Glucose-responsive co-IP/ChIP/O-GlcNAc assays for ChREBP; HSP90 inhibition with HCFC1 degradation and cell-cycle gene readouts

    PMID:31227231 PMID:31693902

    Open questions at the time
    • Whether metabolic and cell-cycle programs are regulated by the same HCF-1 pool unclear
  13. 2024 Medium

    Extended the promoter-recruitment and tissue-specific roles of HCF-1 to ribosome biogenesis, meiotic entry, neuronal differentiation, and OGT stability, defining context-specific gene programs.

    Evidence Mouse conditional knockouts with RNA-seq, ChIP at meiotic/neuronal/MMACHC/ribosomal loci, KDM2A and SET-26 co-IP/epistasis, and OGT inhibitor phenocopy

    PMID:35013307 PMID:38485937 PMID:39160277 PMID:40754593 PMID:41651253

    Open questions at the time
    • Whether these tissue programs share a common HCF-1 mechanism unresolved
    • Direct vs complex-mediated recruitment in several contexts inferred from co-IP
  14. 2025 Medium

    Connected HCF-1 to autophagy/lysosomal control via TFEB transcription and to protein-stability regulation of ASXL1, broadening its regulatory outputs.

    Evidence Co-IP and overexpression with autophagy/lysosomal and senescence readouts; ChIP at TFEB promoter; stability/co-IP assays for ASXL1 with BAP1 competition

    PMID:39461872 PMID:39985193 PMID:41968849

    Open questions at the time
    • TFEB axis mostly shown via overexpression/inhibitor perturbation
    • Functional consequence of HCF-1-driven ASXL1 turnover in vivo undefined

Open questions

Synthesis pass · forward-looking unresolved questions
  • How HCF-1 selects among its many partner factors and chromatin-modifier complexes to execute distinct programs (G1/S vs mitosis, proliferation vs lipogenesis vs autophagy) in a given cell remains unresolved.
  • No unified model of partner-selection logic
  • Quantitative contribution of OGT-cleavage, O-GlcNAcylation, HSP90, and BAP1 to context-specific outputs not integrated
  • Structural basis of full HCF-1N:HCF-1C:partner assemblies on chromatin lacking

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 5 GO:0003677 DNA binding 3 GO:0060090 molecular adaptor activity 3 GO:0098772 molecular function regulator activity 2
Localization
GO:0005694 chromosome 3 GO:0005634 nucleus 2 GO:0005794 Golgi apparatus 1 GO:0005829 cytosol 1
Pathway
R-HSA-1266738 Developmental Biology 4 R-HSA-1640170 Cell Cycle 4 R-HSA-4839726 Chromatin organization 4 R-HSA-74160 Gene expression (Transcription) 4 R-HSA-392499 Metabolism of proteins 3
Partners
ASXL1BAP1CHREBPE2F1HSP90OGTTHAP11ZNF143
Complex memberships
Set1/Ash2 H3K4 methyltransferase complexSin3 HDAC complexTHAP11/ZNF143/HCFC1 complex

Evidence

Reading pass · 50 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2003 Separate regions of HCF-1 critical for cell proliferation associate with the Sin3 histone deacetylase (HDAC) complex and a human trithorax-related Set1/Ash2 histone H3-K4 methyltransferase (HMT) complex; HCF-1 tethers these two complexes together, and the transcriptional activator VP16 selectively binds HCF-1 associated with the Set1/Ash2 HMT complex in the absence of the Sin3 HDAC complex. Co-immunoprecipitation, mass spectrometry, in vitro binding assays, domain mapping Genes & development High 12670868
2001 HCF-1 is naturally bound to chromatin in uninfected cells through its VP16 interaction domain (Kelch/beta-propeller domain); dissociation from chromatin in tsBN67 cells precedes and causes temperature-induced cell proliferation arrest. Chromatin fractionation, temperature-shift experiments, tsBN67 cell proliferation assays Molecular and cellular biology High 11340173
2011 O-GlcNAc transferase (OGT) both O-GlcNAcylates the HCF-1N subunit and directly cleaves HCF-1 at the HCF-1PRO repeat sequences, performing site-specific proteolytic maturation; replacement of HCF-1PRO repeats with a heterologous cleavage signal promotes proteolysis but fails to activate HCF-1C M-phase functions, showing that OGT-mediated cleavage is specifically required for HCF-1C function. In vitro OGT cleavage assays, mutagenesis of HCF-1PRO repeats, cell-based M-phase progression assays Cell High 21295698
2013 The tetratricopeptide-repeat (TPR) domain of OGT binds the C-terminal portion of an HCF-1 proteolytic repeat, positioning the cleavage region in the glycosyltransferase active site above UDP-GlcNAc; cleavage occurs between cysteine and glutamate residues producing a pyroglutamate product; mutation of the cleavage-site glutamate to serine converts an HCF-1 proteolytic repeat into a glycosylation substrate. Crystal structure of OGT:HCF-1PRO-repeat complex, active-site mutagenesis, biochemical cleavage assays Science High 24311690
2003 HCF-1 regulates two distinct stages of the cell cycle via its two proteolytically generated subunits: HCF-1N promotes G1-phase progression, while HCF-1C ensures proper cytokinesis/exit from mitosis; siRNA depletion of HCF-1 in diverse mammalian cells caused both G1 arrest and cytokinesis defects, and proteolytic processing is required to separate and ensure these functions. siRNA knockdown in multiple mammalian cell lines, cell-cycle analysis, cytokinesis assays, expression of separated subunits The EMBO journal High 12743030
2004 Depletion of the HCF-1C subunit causes mitotic defects including a switch from monomethyl to dimethyl H4-K20 and defective chromosome alignment/segregation; HCF-1C regulates expression of the H4-K20 methyltransferase PR-Set7, and upregulation of PR-Set7 upon HCF-1 loss leads to improper mitotic H4-K20 methylation and cytokinesis defects. siRNA depletion, Western blot for histone modifications, immunofluorescence, PR-Set7 overexpression rescue experiments Molecular cell High 15200950
2007 During the G1-to-S phase transition, HCF-1 associates with both activator E2F1/E2F3a and repressor E2F4 proteins; when bound to E2F1, HCF-1 acts as a coactivator and recruits MLL and Set-1 histone H3K4 methyltransferases to E2F-responsive promoters, inducing histone methylation and transcriptional activation. Co-immunoprecipitation, chromatin immunoprecipitation (ChIP), cell-cycle-staged cell fractionation, reporter assays Molecular cell High 17612494
2009 BAP1 deubiquitinase interacts with HCF-1N via an HCF-1 binding motif (HBM); BAP1 deubiquitinates Lys-48-linked polyubiquitin chains on the Kelch domain of HCF-1N; the HBM of BAP1 is required for both the HCF-1 interaction and BAP1-mediated growth regulation, and dominant-negative BAP1-mediated growth suppression is entirely dependent on the HBM. Mass spectrometry of co-purified proteins, co-immunoprecipitation, RNAi depletion, ubiquitin assays, HBM point mutants The Journal of biological chemistry High 19815555
2000 HCF-1 contains two matched pairs of self-association sequences (SAS1 and SAS2) mediating HCF-1N:HCF-1C subunit association; SAS1 consists of a 43-aa HCF-1N region that associates with a tandem pair of fibronectin type 3 (Fn3) repeats in HCF-1C; HCF-1C subunits recruit HCF-1N subunits to the nucleus via a C-terminal nuclear localization signal. Domain deletion analysis, co-immunoprecipitation, nuclear localization assays Molecular and cellular biology High 10958670
2012 Crystal structure of the HCF-1 self-association sequence 1 (SAS1) reveals an interdigitated fibronectin type 3 (Fn3) tandem repeat structure formed by SAS1 elements from HCF-1N and HCF-1C; the C-terminal nuclear localization signal (NLS) recruited by this structure is required for formation of the VP16-induced transcriptional regulatory complex. X-ray crystallography, mutagenesis of NLS, VP16-induced complex formation assays Proceedings of the National Academy of Sciences High 23045687
2008 C. elegans HCF-1 physically associates with the DAF-16/FOXO transcription factor; loss of hcf-1 causes daf-16-dependent lifespan extension and heightened stress resistance; HCF-1 limits DAF-16 recruitment to target gene promoters and thereby represses a subset of DAF-16-regulated genes. Co-immunoprecipitation, ChIP, genetic epistasis (daf-16 mutant suppression), lifespan assays, gene expression profiling PLoS biology High 18828672
2011 C. elegans HCF-1 acts downstream of SIR-2.1 in lifespan regulation; SIR-2.1/SIRT1 and HCF-1 form protein complexes in both worms and mammalian cells; 80% overlap in DAF-16 target genes regulated by hcf-1 mutation and sir-2.1 overexpression; mammalian HCF-1 also represses FOXO/SIRT1 target genes, demonstrating conservation of this regulatory axis. Co-immunoprecipitation, genetic epistasis analysis, gene expression profiling, lifespan assays PLoS genetics High 21909281
2010 THAP1 binds HCF-1 in vitro and associates with HCF-1 and OGT in vivo via a consensus HCF-1 binding motif (HBM); endogenous THAP1 mediates recruitment of HCF-1 to the RRM1 promoter during endothelial cell proliferation; HCF-1 is essential for transcriptional activation of RRM1. Proteomic analysis, in vitro binding, co-immunoprecipitation, ChIP, RNAi knockdown, reporter assays The Journal of biological chemistry High 20200153
2010 THAP11 (Ronin) binds with HCF-1 to a hyperconserved enhancer element at promoters of genes involved in transcription initiation, mRNA splicing, and cell metabolism in embryonic stem cells; Ronin/HCF-1 binding leads to both repression and activation of target genes essential for protein biosynthesis and energy production. ChIP-seq, co-immunoprecipitation, RNAi knockdown, gene expression profiling Genes & development High 20581084
2013 In HeLa cells, HCFC1 is bound to ~5,400 active CpG-island promoters; ZNF143, THAP11, YY1, and GABP transcription factors co-localize with HCFC1 at ~90% of HCFC1-bound promoters, revealing that a small set of sequence-specific factors directs HCFC1 to active promoters. ChIP-seq, motif analysis, co-localization analysis Genome research Medium 23539139
2002 The highly conserved C-terminal WYF domain of HCF-1 interacts with the MYND domain of PDCD2; overexpression of PDCD2 suppresses HCF-1 complementation of the tsBN67 temperature-sensitive proliferation defect; expression of interfering domains of either protein enhances complementation, defining PDCD2 as a negative regulator of HCF-1C. Co-immunoprecipitation, domain mapping, tsBN67 cell complementation assay Oncogene Medium 12149646
2002 HCF-1 is a component of spliceosomal complexes; it interacts with U1 and U5 splicing snRNPs; the tsBN67 HCF-1 missense mutation disrupts interaction with snRNPs at non-permissive temperature, causing inefficient spliceosome assembly and inhibition of splicing; restoration of wild-type HCF-1 rescues splicing. Co-immunoprecipitation with snRNPs, in vitro splicing assays in nuclear extracts, tsBN67 temperature-shift experiments, rescue by wild-type HCF-1 expression The EMBO journal Medium 12456665
2002 HCF-1 contains an activation domain (HCF-1AD) in its C-terminal subunit required for maximal transactivation by VP16 and cellular LZIP; p300 augments HCF-1AD activity; cells lacking HCF-1AD show reduced HSV immediate-early gene expression and lower viral titers. Reporter gene assays, domain deletion/mutagenesis, infection assays, p300 co-expression Proceedings of the National Academy of Sciences Medium 12271126
2002 HCF-1 beta-propeller domain binds a new cellular protein HPIP, which contains an HCF-binding motif and a leucine-rich nuclear export sequence; HPIP shuttles between nucleus and cytoplasm in a CRM1-dependent manner; HPIP overexpression leads to accumulation of HCF-1 in the cytoplasm, suggesting HPIP regulates HCF-1 subcellular localization. Co-immunoprecipitation, subcellular fractionation, CRM1 inhibition (leptomycin B), overexpression studies The Journal of biological chemistry Medium 12235138
2006 The HCF-1 proteolytic processing domain interacts with FHL2 (four-and-a-half LIM domain-2); FHL2 interacts exclusively with the non-processed HCF-1 precursor; FHL2 and HCF-1 co-stimulate transcription of an HCF-1-dependent target gene; thus, site-specific proteolysis of HCF-1 regulates its interaction with FHL2 and modulates coactivator activity. Co-immunoprecipitation, reporter gene assay, domain analysis with processed vs. unprocessed HCF-1 Proceedings of the National Academy of Sciences Medium 16624878
2010 HCF-1 localizes to the Golgi apparatus in unstimulated sensory neurons; upon Golgi disruption, HCF-1 rapidly relocalizes to the nucleus, unlike other Golgi-associated proteins; this Golgi sequestration is distinct from the previously proposed ER/CREB3-mediated cytoplasmic retention, and nuclear relocalization correlates with viral reactivation. Immunofluorescence in primary neurons and latently infected mice, Golgi disruption experiments, subcellular localization studies Journal of virology Medium 18667495
2010 HCF-1 interacts directly and simultaneously with both HSV DNA replication proteins and the cellular histone chaperone Asf1b; Asf1b localizes with HCF-1 at viral replication foci; depletion of Asf1b results in significantly reduced viral DNA accumulation, establishing HCF-1 as a component of the HSV DNA replication assembly that promotes viral DNA replication by coupling Asf1b to replication components. Co-immunoprecipitation (direct and simultaneous), immunofluorescence colocalization, siRNA depletion of Asf1b with viral DNA accumulation readout Proceedings of the National Academy of Sciences Medium 20133788
2007 Loss of the C. elegans HCF-1 homolog (Ce HCF-1) at reduced temperatures causes embryonic lethality with mitotic and cytokinetic defects; viable mutant embryos display reduced levels of phospho-histone H3 serine 10 (H3S10P); mammalian cells with defective HCF-1 also display defects in mitotic H3S10P status, indicating a conserved role for HCF-1 in regulating mitotic histone phosphorylation. C. elegans deletion mutant analysis, immunofluorescence for H3S10P in worms and mammalian cells, tsBN67 temperature-shift experiments PloS one Medium 18043729
2002 Inactivation of pRb family members (pRb, p107, p130) by SV40 large T antigen or adenovirus E1A bypasses the requirement for HCF-1 function in tsBN67 cell proliferation and cytokinesis, without restoring HCF-1 chromatin association; this epistasis indicates that HCF-1 regulates cell proliferation and cytokinesis at least in part by opposing pRb family member function. Genetic epistasis using SV40 Tag and E1A, tsBN67 complementation assays, pRb family member mutants Molecular and cellular biology Medium 12215534
2013 Missense mutations in the HCFC1 Kelch domain cause X-linked cblX disorder; siRNA-mediated knockdown of HCFC1 in fibroblasts leads to coordinate downregulation of MMACHC mRNA; consensus HCFC1 binding sites were identified in the MMACHC promoter, establishing HCFC1 as a transcriptional regulator of MMACHC expression. siRNA knockdown, RT-PCR, promoter binding site analysis, patient fibroblast studies American journal of human genetics Medium 24011988
2014 Zebrafish hcfc1b regulates cranial neural crest cell differentiation and proliferation within posterior pharyngeal arches; hcfc1b-mediated craniofacial abnormalities were rescued by expression of human MMACHC, establishing that HCFC1 acts upstream of MMACHC in craniofacial development. Zebrafish morpholino knockdown, rescue by MMACHC expression, analysis of neural crest cell differentiation/proliferation Developmental biology Medium 25281006
2019 HCF-1 is O-GlcNAcylated in response to glucose as a prerequisite for its binding to ChREBP; upon binding, HCF-1 recruits OGT to O-GlcNAcylate ChREBP and activate it; the HCF-1:ChREBP complex resides at lipogenic gene promoters where HCF-1 regulates H3K4 trimethylation and recruits the histone demethylase PHF2 for epigenetic activation of lipogenic genes. Co-immunoprecipitation, ChIP, O-GlcNAcylation assays, glucose-responsive cell culture experiments, genetic knockdown Molecular cell High 31227231
2016 OGT-mediated glycosylation and HCF-1 proteolysis occur through separable mechanisms within the same active site; a specific TPR domain contact with HCF-1 substrate is critical for proteolysis but not Ser/Thr glycosylation; key catalytic domain residues and UDP-GlcNAc oxygen important for glycosylation are irrelevant for proteolysis; single-activity OGT enzymes (either glycosylase-only or protease-only) can be engineered in vitro and in vivo. Active-site mutagenesis, in vitro glycosylation and proteolysis assays, engineered OGT variants Genes & development High 27056667
2015 The HCF-1PRO repeat cleavage signal has specific OGT-binding properties; the glutamate at the cleavage site inhibits OGT:UDP-GlcNAc association; a novel OGT-binding sequence adjacent to the first HCF-1PRO repeat enhances cleavage, demonstrating that distinct OGT-binding sites in HCF-1 cooperate to promote proteolysis. In vitro OGT binding assays, mutagenesis of cleavage site glutamate, biochemical cleavage assays PloS one Medium 26305326
2018 The HCF-1PRO repeat threonine-rich region is tightly bound by the OGT TPR region and activates both OGT glycosylation and proteolysis activities; linkage of this region to heterologous sequences potentiates serine glycosylation with poor OGT co-substrates and enables proteolysis of non-HCF-1PRO cleavage sequences containing an appropriately positioned glutamate. In vitro OGT glycosylation and proteolysis assays with chimeric substrates, mutagenesis The Journal of biological chemistry Medium 30224358
2009 During the G1-to-S transition, E2F1 associates with HCF-1 through a short DHQY sequence; this HCF-1-binding sequence permits E2F1 to stimulate both DNA damage and apoptosis; HCF-1 and MLL family H3K4 methyltransferases have important functions in E2F1-mediated apoptosis; sequence changes in the E2F1 HCF-1-binding site modulate E2F1-induced apoptosis. Mutagenesis of E2F1 DHQY motif, co-immunoprecipitation, apoptosis assays, DNA damage assays, HCF-1 and MLL knockdown The EMBO journal Medium 19763085
2003 The HCF-1 binding motif (HBM) occurs in a wide spectrum of DNA-binding proteins and cofactors; Krox20 and E2F4 show strong requirement for functional HCF-1 to activate transcription; in Krox20, the HBM lies in the N-terminal activation domain and its mutation diminishes both transactivation and association with the HCF-1 beta-propeller; the HCF-1C activation domain contributes to Krox20-mediated activation, possibly through recruitment of p300. Reporter gene assays, co-immunoprecipitation, mutagenesis of HBM in Krox20 The Journal of biological chemistry Medium 14532282
2012 THAP11 physically associates with HCF-1 and recruits HCF-1 to target gene promoters in colon cancer cells; THAP11-mediated gene regulation and chromatin association require HCF-1, while HCF-1 recruitment at THAP11 target genes requires THAP11, demonstrating mutual dependence. Co-immunoprecipitation, ChIP, siRNA knockdown of THAP11 and HCF-1, gene expression profiling Molecular and cellular biology Medium 22371484
2015 The THAP11/ZNF143/HCFC1 complex is recruited to chromatin through the ACTACA submotif shared by THAP11 and ZNF143; its position, spacing, and orientation relative to the ZNF143 core motif are critical for THAP11 and HCFC1 recruitment to ZNF143-occupied loci; CRISPR-Cas9-mediated alteration of the ACTACA submotif at endogenous promoters reduces THAP11 and HCFC1 binding and alters target gene transcription and histone modifications. CRISPR-Cas9 mutagenesis at endogenous promoters, synthetic chromatin-integrated constructs, ChIP, gene expression analysis Molecular and cellular biology High 26416877
2019 HSP90 is required for the stability of nuclear HCFC1; HSP90 is required to maintain expression of HCFC1-targeted cell-cycle genes; HSP90 inhibition leads to HCFC1 degradation and consequent downregulation of cell-cycle gene expression. Three independent systematic analyses, biochemical co-immunoprecipitation, HSP90 inhibitor treatment, HCFC1 depletion with cell-cycle gene expression analysis Cell reports Medium 31693902
2016 HCF-1 conditional knockout in mouse hepatocytes demonstrates that HCF-1 is required for cell-cycle re-entry and proliferation in resting adult liver cells; HCF-1-deficient hepatocytes fail to re-enter the cell cycle during liver regeneration; in embryos, epiblast-specific HCF-1 loss causes cell-cycle exit and apoptosis of HCF-1-negative cells by E8.5. Cre-inducible conditional knockout mouse model, liver regeneration model, BrdU incorporation, apoptosis assays, X-chromosome inactivation analysis Developmental biology High 26921005
2016 Complete epiblast-specific loss of HCF-1 in male embryos leads to developmental arrest at E6.5 with rapid progressive cell-cycle exit, failure of anterior visceral endoderm migration, failure of primitive streak formation, and absence of gastrulation; the pattern of lethality resembles loss of β-catenin function. Conditional knockout mouse model (Hcfc1 epiKO/Y), developmental staging, immunohistochemistry, cell-cycle analysis Developmental biology High 27521049
2021 SETD5 regulates RNA polymerase II promoter-proximal pausing on E2F target genes in hematopoietic stem cells in cooperation with HCF-1 and the PAF1 complex; loss of Setd5 disrupts HSC quiescence; HCF-1 co-immunoprecipitates with SETD5. Co-immunoprecipitation of SETD5 and HCF-1, conditional knockout mouse model, Pol II ChIP, transcriptome analysis Leukemia Medium 34853439
2020 HCF-1 activates CDC42 expression by binding to the -881 to -575 region upstream of the CDC42 transcription start site; overexpression of constitutively active CDC42F28L rescues G1 phase delay and multinucleate defects caused by HCF-1 loss, establishing CDC42 as a functional downstream target of HCF-1 in cell cycle progression. ChIP to the CDC42 promoter, siRNA depletion of HCF-1, rescue by constitutively active CDC42, cell cycle and multinucleation assays Cell death & disease Medium 33097698
2013 HCF-1 is required for INS-1 pancreatic β-cell glucose-stimulated insulin secretion; HCF-1 reduction causes decreased expression of Pdx1; HCF-1 and E2F1 co-localize at the Pdx1 promoter as shown by ChIP. siRNA knockdown, glucose-stimulated insulin secretion assay, RT-PCR for Pdx1, ChIP PloS one Medium 24250814
2024 In C. elegans, HCF-1 chromatin localization is largely dependent on functional SET-26; SET-26 and HCF-1 cooperate to regulate a common set of target genes; the histone deacetylase HDA-1 opposes both SET-26 and HCF-1 at a subset of shared target genes and in longevity regulation. ChIP, genetic epistasis (set-26, hcf-1, hda-1 mutants), gene expression profiling, lifespan assays Nature communications Medium 38485937
2022 Mouse models of Hcfc1 mutation exhibit reduced expression of MMACHC (confirming transcriptional regulation) and additionally show reduced expression of ribosomal protein subunit genes; developmental defects associated with these mutations include aspects of both cblC and ribosomopathies, identifying HCFC1/RONIN as transcriptional regulators of ribosome biogenesis during development. Mouse conditional knockout models, RNA-seq, metabolic analysis, developmental phenotyping Nature communications Medium 35013307
2023 Conditional deletion of HCF-1 in sensory neurons in vivo causes a striking reduction in latently infected neurons that initiate HSV-1 reactivation; this correlated with a defect in removal of repressive heterochromatin from latent viral genomes, establishing HCF-1 as a critical in vivo regulator that promotes the transition of latent HSV genomes from a repressed chromatin state. HCF-1 conditional knockout mouse model, HSV latency/reactivation model, ChIP for repressive chromatin marks, viral reactivation quantification mBio High 36692302
2025 Hepatocyte-specific HCF-1 deletion leads to progressive loss of OGT protein levels and global O-GlcNAcylation without altering OGT mRNA, indicating post-translational regulation of OGT stability by HCF-1; loss of HCF-1 reduces nuclear OGT and O-GlcNAcylation, mimicking fasting conditions; HCF-1-negative hepatocytes display cytoplasmic O-GlcNAcylation while HCF-1-positive cells maintain nuclear localization. Hepatocyte-specific conditional knockout mouse, immunofluorescence, Western blot, OGT mRNA analysis, fractionation studies Scientific reports Medium 40754593
2026 HCF-1 is required for neuronal differentiation and forebrain commissure formation; HCF-1 directly occupies promoters of key neuronal genes (Elavl3, NeuroD1) and its loss reduces activating chromatin marks at these loci; OGT inhibition phenocopies HCF-1 depletion in impairing neuronal proliferation, differentiation, and neurite outgrowth; glycoproteomic analysis reveals disruption of OGT-dependent protein networks involved in neuronal structure. Conditional neuronal knockout, ChIP at neuronal gene promoters, siRNA depletion, OGT inhibitor (OSMI-1), glycoproteomics, transcriptomics Neurobiology of disease Medium 41651253
2026 HCF-1 binds to the C-terminal ~200 amino acids of ASXL1 and promotes ASXL1 proteasome-dependent turnover; deletion of this ASXL1 C-terminal region abrogates HCF-1 binding and stabilizes ASXL1; HCF-1 and BAP1 show reciprocal antagonism in association with ASXL1, suggesting indirect coupling in complex assembly. P2A dual-reporter stability assay, co-immunoprecipitation, proteasome inhibitor experiments, domain deletion mapping FASEB journal Medium 41968849
2024 KDM2A recruits E2F1 and HCFC1 to promoters of key meiosis genes (Stra8, Meiosin, Spo11, Sycp1) in male germ cells; conditional deletion of Kdm2a disrupts H3K36me2/3 deposition and impairs expression of HCFC1-recruited target genes required for meiotic entry and progression. Co-immunoprecipitation of KDM2A-E2F1-HCFC1, ChIP at meiotic gene promoters, conditional knockout mouse, H3K36me2/3 analysis The EMBO journal Medium 39160277
1999 A second HCF-like protein HCF-2 was identified; chimeric protein analysis showed that differences between the fifth and sixth kelch repeats of the beta-propeller domains of HCF-1 and HCF-2 determine selective recruitment of HCF-1 over HCF-2 by VP16 and LZIP. Chimeric protein construction, in vitro binding assays, VP16-induced complex assembly assays Journal of virology Medium 10196288
2025 RONIN (THAP11) modulates TFEB transcriptional activity through its interaction with HCF-1/HCFC1; RONIN overexpression improved autophagy levels, lysosomal activity, and attenuated D-galactose-induced hair cell senescence, working through TFEB activation. Co-immunoprecipitation of RONIN and HCF1, overexpression studies, autophagy/lysosomal activity assays, hair cell senescence model Advanced science Medium 39985193
2024 HSP90 N-terminal inhibition reduces HCFC1 protein levels, preventing HCFC1 from binding to the TFEB proximal promoter; decreased TFEB transcription then reduces LC3 levels and promotes mitochondria-derived vesicle (MDV) formation and tumor metastasis; re-activation of the HCFC1-TFEB-LC3 axis by blocking MDV formation suppresses metastasis. ChIP for HCFC1 at TFEB promoter, HSP90 inhibitor treatment, HSP90AA1-HCFC1 co-immunoprecipitation, TFEB/LC3 Western blot, MDV formation assays Autophagy Medium 39461872

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2003 Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1. Genes & development 337 12670868
2009 The deubiquitinating enzyme BAP1 regulates cell growth via interaction with HCF-1. The Journal of biological chemistry 226 19815555
2011 O-GlcNAc transferase catalyzes site-specific proteolysis of HCF-1. Cell 210 21295698
2007 E2F activation of S phase promoters via association with HCF-1 and the MLL family of histone H3K4 methyltransferases. Molecular cell 208 17612494
2001 Loss of HCF-1-chromatin association precedes temperature-induced growth arrest of tsBN67 cells. Molecular and cellular biology 168 11340173
2013 HCF-1 is cleaved in the active site of O-GlcNAc transferase. Science (New York, N.Y.) 160 24311690
2003 Proteolytic processing is necessary to separate and ensure proper cell growth and cytokinesis functions of HCF-1. The EMBO journal 113 12743030
2013 An X-linked cobalamin disorder caused by mutations in transcriptional coregulator HCFC1. American journal of human genetics 105 24011988
2010 Ronin/Hcf-1 binds to a hyperconserved enhancer element and regulates genes involved in the growth of embryonic stem cells. Genes & development 99 20581084
2008 Caenorhabditis elegans HCF-1 functions in longevity maintenance as a DAF-16 regulator. PLoS biology 95 18828672
2011 The evolutionarily conserved longevity determinants HCF-1 and SIR-2.1/SIRT1 collaborate to regulate DAF-16/FOXO. PLoS genetics 93 21909281
2010 The THAP-zinc finger protein THAP1 associates with coactivator HCF-1 and O-GlcNAc transferase: a link between DYT6 and DYT3 dystonias. The Journal of biological chemistry 91 20200153
2012 A noncoding, regulatory mutation implicates HCFC1 in nonsyndromic intellectual disability. American journal of human genetics 88 23000143
2013 HCFC1 is a common component of active human CpG-island promoters and coincides with ZNF143, THAP11, YY1, and GABP transcription factor occupancy. Genome research 84 23539139
2004 A switch in mitotic histone H4 lysine 20 methylation status is linked to M phase defects upon loss of HCF-1. Molecular cell 84 15200950
1996 Species-specific effects of the hcf-1 gene on baculovirus virulence. Journal of virology 61 8764020
2010 Transcriptional coactivator HCF-1 couples the histone chaperone Asf1b to HSV-1 DNA replication components. Proceedings of the National Academy of Sciences of the United States of America 57 20133788
2012 A transcriptional regulatory role of the THAP11-HCF-1 complex in colon cancer cell function. Molecular and cellular biology 56 22371484
2009 E2F1 mediates DNA damage and apoptosis through HCF-1 and the MLL family of histone methyltransferases. The EMBO journal 55 19763085
2003 HCF-1 functions as a coactivator for the zinc finger protein Krox20. The Journal of biological chemistry 54 14532282
2015 HCFC1 loss-of-function mutations disrupt neuronal and neural progenitor cells of the developing brain. Human molecular genetics 51 25740848
2019 HCF-1 Regulates De Novo Lipogenesis through a Nutrient-Sensitive Complex with ChREBP. Molecular cell 46 31227231
1995 The gene encoding the VP16-accessory protein HCF (HCFC1) resides in human Xq28 and is highly expressed in fetal tissues and the adult kidney. Genomics 45 7789979
2012 Genomic and physiological characterization of the chromate-reducing, aquifer-derived Firmicute Pelosinus sp. strain HCF1. Applied and environmental microbiology 44 23064329
2001 The hydrophobin HCf-1 of Cladosporium fulvum is required for efficient water-mediated dispersal of conidia. Fungal genetics and biology : FG & B 44 11343402
2002 PDCD2 is a negative regulator of HCF-1 (C1). Oncogene 42 12149646
2000 HCF-1 amino- and carboxy-terminal subunit association through two separate sets of interaction modules: involvement of fibronectin type 3 repeats. Molecular and cellular biology 42 10958670
2009 Recruitment of the transcriptional coactivator HCF-1 to viral immediate-early promoters during initiation of reactivation from latency of herpes simplex virus type 1. Journal of virology 41 19570863
2004 Combinatorial transcription of herpes simplex virus and varicella zoster virus immediate early genes is strictly determined by the cellular coactivator HCF-1. The Journal of biological chemistry 41 15522876
2013 The dynamics of HCF-1 modulation of herpes simplex virus chromatin during initiation of infection. Viruses 40 23698399
1999 Herpes simplex virus transactivator VP16 discriminates between HCF-1 and a novel family member, HCF-2. Journal of virology 40 10196288
2014 Hcfc1b, a zebrafish ortholog of HCFC1, regulates craniofacial development by modulating mmachc expression. Developmental biology 36 25281006
2002 An activation domain in the C-terminal subunit of HCF-1 is important for transactivation by VP16 and LZIP. Proceedings of the National Academy of Sciences of the United States of America 36 12271126
2022 Traditional Chinese medicine CFF-1 exerts a potent anti-tumor immunity to hinder tumor growth and metastasis in prostate cancer through EGFR/JAK1/STAT3 pathway to inhibit PD-1/PD-L1 checkpoint signaling. Phytomedicine : international journal of phytotherapy and phytopharmacology 30 35172257
2008 Association of the cellular coactivator HCF-1 with the Golgi apparatus in sensory neurons. Journal of virology 27 18667495
2022 Mutations in Hcfc1 and Ronin result in an inborn error of cobalamin metabolism and ribosomopathy. Nature communications 26 35013307
2015 Multiple congenital anomalies in two boys with mutation in HCFC1 and cobalamin disorder. European journal of medical genetics 26 25595573
2007 Contribution of VCAF-positive cells to neovascularization and calcification in atherosclerotic plaque development. The Journal of pathology 26 17154367
2015 A novel HCFC1 variant in male siblings with intellectual disability and microcephaly in the absence of cobalamin disorder. Biomedical reports 25 26893841
2002 Spontaneous reversion of tsBN67 cell proliferation and cytokinesis defects in the absence of HCF-1 function. Experimental cell research 25 12061822
1998 Co-suppression of the hydrophobin gene HCf-1 is correlated with antisense RNA biosynthesis in Cladosporium fulvum. Molecular & general genetics : MGG 25 9819056
2018 Traditional Chinese Medicine CFF-1 induced cell growth inhibition, autophagy, and apoptosis via inhibiting EGFR-related pathways in prostate cancer. Cancer medicine 24 29533017
2010 Role of the HCF-1 basic region in sustaining cell proliferation. PloS one 24 20126307
2002 Inactivation of the retinoblastoma protein family can bypass the HCF-1 defect in tsBN67 cell proliferation and cytokinesis. Molecular and cellular biology 23 12215534
2019 Heat-Shock Protein 90 Controls the Expression of Cell-Cycle Genes by Stabilizing Metazoan-Specific Host-Cell Factor HCFC1. Cell reports 22 31693902
2015 Distinct OGT-Binding Sites Promote HCF-1 Cleavage. PloS one 22 26305326
2007 Epigenetic regulation of histone H3 serine 10 phosphorylation status by HCF-1 proteins in C. elegans and mammalian cells. PloS one 22 18043729
2015 Genomic Determinants of THAP11/ZNF143/HCFC1 Complex Recruitment to Chromatin. Molecular and cellular biology 21 26416877
1997 HCF-1, a hydrophobin from the tomato pathogen Cladosporium fulvum. Gene 21 9249071
2016 Proteolysis of HCF-1 by Ser/Thr glycosylation-incompetent O-GlcNAc transferase:UDP-GlcNAc complexes. Genes & development 20 27056667
2014 Royal Jelly-Mediated Prolongevity and Stress Resistance in Caenorhabditis elegans Is Possibly Modulated by the Interplays of DAF-16, SIR-2.1, HCF-1, and 14-3-3 Proteins. The journals of gerontology. Series A, Biological sciences and medical sciences 20 25073462
2006 Site-specific proteolysis of the transcriptional coactivator HCF-1 can regulate its interaction with protein cofactors. Proceedings of the National Academy of Sciences of the United States of America 20 16624878
2002 Interaction of HCF-1 with a cellular nuclear export factor. The Journal of biological chemistry 20 12235138
2025 RONIN/HCF1-TFEB Axis Protects Against D-Galactose-Induced Cochlear Hair Cell Senescence Through Autophagy Activation. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 19 39985193
2002 A novel function for human factor C1 (HCF-1), a host protein required for herpes simplex virus infection, in pre-mRNA splicing. The EMBO journal 19 12456665
2004 A protein sequestering system reveals control of cellular programs by the transcriptional coactivator HCF-1. The Journal of biological chemistry 18 15190068
1994 Genomic organization of the human VP16 accessory protein, a housekeeping gene (HCFC1) mapping to Xq28. Genomics 17 7829097
2020 HCF-1 promotes cell cycle progression by regulating the expression of CDC42. Cell death & disease 16 33097698
2020 The role of HCFC1 in syndromic and non-syndromic intellectual disability. Medical research archives 16 34164576
2018 The Cellular Coactivator HCF-1 Is Required for Glucocorticoid Receptor-Mediated Transcription of Bovine Herpesvirus 1 Immediate Early Genes. Journal of virology 16 29899098
2016 Compensatory embryonic response to allele-specific inactivation of the murine X-linked gene Hcfc1. Developmental biology 15 26921005
2016 HCF1 and OCT2 Cooperate with EBNA1 To Enhance OriP-Dependent Transcription and Episome Maintenance of Latent Epstein-Barr Virus. Journal of virology 15 27009953
2018 The conserved threonine-rich region of the HCF-1PRO repeat activates promiscuous OGT:UDP-GlcNAc glycosylation and proteolysis activities. The Journal of biological chemistry 13 30224358
2016 Epiblast-specific loss of HCF-1 leads to failure in anterior-posterior axis specification. Developmental biology 13 27521049
2021 SETD5 modulates homeostasis of hematopoietic stem cells by mediating RNA Polymerase II pausing in cooperation with HCF-1. Leukemia 12 34853439
2017 X-Linked Cobalamin Disorder (HCFC1) Mimicking Nonketotic Hyperglycinemia With Increased Both Cerebrospinal Fluid Glycine and Methylmalonic Acid. Pediatric neurology 12 28363510
2017 HCF-1 encoded by baculovirus AcMNPV is required for productive nucleopolyhedrovirus infection of non-permissive Tn368 cells. Scientific reports 12 28630398
2024 HSP90 N-terminal inhibition promotes mitochondria-derived vesicles related metastasis by reducing TFEB transcription via decreased HSP90AA1-HCFC1 interaction in liver cancer. Autophagy 11 39461872
2023 HCFC1 variants in the proteolysis domain are associated with X-linked idiopathic partial epilepsy: Exploring the underlying mechanism. Clinical and translational medicine 11 37264743
2021 Novel exon-skipping variant disrupting the basic domain of HCFC1 causes intellectual disability without metabolic abnormalities in both male and female patients. Journal of human genetics 11 33517344
2021 A reinterpretation of critical flicker-frequency (CFF) data reveals key details about light adaptation and normal and abnormal visual processing. Progress in retinal and eye research 11 34506951
2012 HCF-1 self-association via an interdigitated Fn3 structure facilitates transcriptional regulatory complex formation. Proceedings of the National Academy of Sciences of the United States of America 11 23045687
2021 Phenotypic Characterization and Whole-Genome Analysis of a Novel Bacteriophage HCF1 Infecting Citrobacter amalonaticus and C. freundii. Frontiers in microbiology 10 33569047
2019 Cortical and Commissural Defects Upon HCF-1 Loss in Nkx2.1-Derived Embryonic Neurons and Glia. Developmental neurobiology 10 31207118
2024 The chromatin factors SET-26 and HCF-1 oppose the histone deacetylase HDA-1 in longevity and gene regulation in C. elegans. Nature communications 9 38485937
2023 Deletion of the Transcriptional Coactivator HCF-1 In Vivo Impairs the Removal of Repressive Heterochromatin from Latent HSV Genomes and Suppresses the Initiation of Viral Reactivation. mBio 9 36692302
2020 MLL5, a histone modifying enzyme, regulates androgen receptor activity in prostate cancer cells by recruiting co-regulators, HCF1 and SET1. BMB reports 8 33050986
2003 Characterization of HCF-1, a determinant of Autographa californica multiple nucleopolyhedrovirus host specificity. Insect molecular biology 8 14986926
1996 The chromosome localization and the HCF repeats of the human host cell factor gene (HCFC1) are conserved in the mouse homologue. Genomics 8 8833156
2005 Expression and mutational analysis of Autographa californica nucleopolyhedrovirus HCF-1: functional requirements for cysteine residues. Journal of virology 7 16254326
2024 Histone demethylase KDM2A recruits HCFC1 and E2F1 to orchestrate male germ cell meiotic entry and progression. The EMBO journal 6 39160277
2014 QF-PCR rapid aneuploidy screen and aCGH analysis of cell free fetal (cff) DNA in supernatant of compromised amniotic fluids (AF). Prenatal diagnosis 5 24801814
1981 On cystic fibrosis factor (CFF) and its proposed influence on mucociliary function. Rhinology 5 7302469
2024 Network pharmacology and experimental evaluation strategies to decipher the underlying pharmacological mechanism of Traditional Chinese Medicine CFF-1 against prostate cancer. Aging 4 38484140
2013 The transcriptional co-regulator HCF-1 is required for INS-1 β-cell glucose-stimulated insulin secretion. PloS one 4 24250814
2026 A SET domain-containing protein and HCF-1 maintain transgenerational epigenetic memory. Nature communications 3 41513691
2025 HCF-1 as a key modulator of OGT function and O-GlcNAcylation in the liver. Scientific reports 3 40754593
2024 Variants in HCFC1 and MN1 genes causing intellectual disability in two Pakistani families. BMC medical genomics 3 38956580
2022 Cell-Free Filtrates (CFF) as Vectors of a Transmissible Pathologic Tissue Memory Code: A Hypothetical and Narrative Review. International journal of molecular sciences 3 36232877
2003 Production and secretion of Aspergillus nidulans catalase B in filamentous fungi driven by the promoter and signal peptide of the Cladosporium fulvum hydrophobin gene hcf-1. Current genetics 3 12955453
2025 Epigenetic co-regulator HCF-1 promotes lung cancer via O-GlcNAcylation-dependent pathways. Molecular therapy. Oncology 2 41018975
2024 Key chromatin regulator-related genes associated with the risk of coronary artery disease regulate the expression of HCFC1, RNF8, TNP1 and SET. Heliyon 2 38596069
2021 THE APPLICATION OF CELL-FREE FETAL DNA (cff-DNA) AND SIBLINGS DNA METHODS IN THE PROCESS OF PATERNITY TEST THROUGH CODIS STR LOCI (CSF1PO, THO1, TPOX, AND vWA). African journal of infectious diseases 2 35047725
1996 The complete sequence of the host cell factor 1 (HCFC1) gene and its promoter: a role for YY1 transcription factor in the regulation of its expression. Genomics 2 8661027
2025 Hcfc1 and Ogt Mediate Zebrafish CNS Regeneration Through Hippo/Yap Signalling. Cell proliferation 1 41108067
2023 The chromatin factors SET-26 and HCF-1 oppose the histone deacetylase HDA-1 in longevity and gene regulation in C. elegans. bioRxiv : the preprint server for biology 1 36993207
2017 [Chinese medicinal compound CFF-1 induces the apoptosis and cycle-arrest of prostate cancer cells via the PI3K/AKT/FOXO1 signaling pathway]. Zhonghua nan ke xue = National journal of andrology 1 29726666
2026 The HCF-1:OGT axis regulates neuronal proliferation and differentiation. Neurobiology of disease 0 41651253
2026 A Degron-Like Regulatory Region in the ASXL1 C-Terminal Domain Mediates HCF-1-Dependent Regulation of Protein Stability. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 0 41968849
2025 The IRβ/HCF-1/ChREBP axis: A new paradigm in insulin-regulated hepatic lipogenesis. Life sciences 0 41161405

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