Affinage

ZFP1

Zinc finger protein 1 homolog · UniProt Q6P2D0

Round 2 corrected
Length
407 aa
Mass
47.5 kDa
Annotated
2026-04-28
95 papers in source corpus 2 papers cited in narrative 2 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ZFP1 encodes a C2H2-type zinc finger protein containing seven zinc finger domains and two helix-turn-helix motifs, structurally related to the Drosophila Krüppel segmentation gene product, and is ubiquitously expressed during mammalian embryogenesis with peak expression at embryonic day 12 (PMID:2574853). Overexpression of ZFP1 promotes proliferation and migration of gastric cancer cells (PMID:39261568). The direct DNA-binding targets, transcriptional regulatory function, and downstream signaling mechanisms of ZFP1 remain uncharacterized.

Mechanistic history

Synthesis pass · year-by-year structured walk · 2 steps
  1. 1989 Medium

    Cloning of the mouse Zfp-1 gene established that it encodes a Krüppel-related C2H2 zinc finger protein with seven zinc fingers and two helix-turn-helix motifs, expressed ubiquitously during embryogenesis — defining ZFP1 as a putative transcriptional regulator active in development.

    Evidence cDNA cloning, sequencing, Northern blot developmental expression profiling, and chromosomal in situ mapping in mouse

    PMID:2574853

    Open questions at the time
    • No DNA-binding targets or transcriptional activity demonstrated
    • Expression data limited to mouse; human orthologue expression not characterized
    • No loss-of-function or gain-of-function experiments performed
  2. 2024 Low

    Functional cell-based assays showed that ZFP1 expression levels positively regulate gastric cancer cell proliferation and migration, providing the first evidence for a role in human cancer cell biology.

    Evidence CCK8, colony formation, and wound-healing assays with ZFP1 overexpression/knockdown in gastric cancer cell lines

    PMID:39261568

    Open questions at the time
    • Single-lab study without independent replication
    • No downstream pathway or direct target genes identified
    • No in vivo tumor model validation

Open questions

Synthesis pass · forward-looking unresolved questions
  • The direct transcriptional targets of ZFP1, its DNA-binding specificity, and the signaling pathways through which it promotes proliferation and migration remain unknown.
  • No ChIP-seq or equivalent DNA-binding data exist
  • No structural or biochemical characterization of the zinc finger domains' binding specificity
  • Physiological role during mammalian development has not been tested by knockout or knockdown studies

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003677 DNA binding 1

Evidence

Reading pass · 2 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1989 The mouse Zfp-1 gene encodes a protein containing 7 C2H2-type zinc fingers and two helix-turn-helix motifs, structurally related to the Drosophila Krüppel segmentation gene product. The Zfp-1 gene is ubiquitously expressed during murine embryogenesis with peak expression at embryonic day 12, and maps to the 16q region of mouse chromosome 8 between the uvomorulin and tyrosine aminotransferase genes. cDNA cloning and sequencing, Northern blot (developmental expression profiling), chromosomal in situ mapping Nucleic acids research Medium 2574853
2024 ZFP1 promotes proliferation and migration of gastric cancer cells, as demonstrated by CCK8, colony formation, and wound-healing assays following manipulation of ZFP1 expression levels. CCK8 cell viability assay, colony formation assay, wound-healing (scratch) assay in gastric cancer cell lines Scientific reports Low 39261568

Source papers

Stage 0 corpus · 95 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2017 Impact of cytosine methylation on DNA binding specificities of human transcription factors. Science (New York, N.Y.) 934 28473536
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2010 Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science (New York, N.Y.) 421 20360068
2005 Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Genome research 409 16344560
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2000 tA single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta. The Plant cell 339 11090207
2000 A telomeric avirulence gene determines efficacy for the rice blast resistance gene Pi-ta. The Plant cell 338 11090206
2014 Genome-wide association study identifies multiple susceptibility loci for pancreatic cancer. Nature genetics 281 25086665
2010 Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation. Nature cell biology 217 20562864
2011 Multiple translocation of the AVR-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species. PLoS pathogens 167 21829350
2008 Systematic identification of mRNAs recruited to argonaute 2 by specific microRNAs and corresponding changes in transcript abundance. PloS one 148 18461144
2019 Systematic bromodomain protein screens identify homologous recombination and R-loop suppression pathways involved in genome integrity. Genes & development 110 31753913
2001 Characterization of PitA and PitB from Escherichia coli. Journal of bacteriology 108 11489853
2018 Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei. Molecular & cellular proteomics : MCP 101 30021884
2003 Germline cell death is inhibited by P-element insertions disrupting the dcp-1/pita nested gene pair in Drosophila. Genetics 100 14704173
2014 Two new insulator proteins, Pita and ZIPIC, target CP190 to chromatin. Genome research 90 25342723
2008 Genome organization and evolution of the AVR-Pita avirulence gene family in the Magnaporthe grisea species complex. Molecular plant-microbe interactions : MPMI 88 18393625
2007 Instability of the Magnaporthe oryzae avirulence gene AVR-Pita alters virulence. Fungal genetics and biology : FG & B 88 17387027
2016 Architectural proteins Pita, Zw5,and ZIPIC contain homodimerization domain and support specific long-range interactions in Drosophila. Nucleic acids research 60 27137890
2004 Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions. Genome research 57 15342556
2021 The Fungal Effector Avr-Pita Suppresses Innate Immunity by Increasing COX Activity in Rice Mitochondria. Rice (New York, N.Y.) 48 33443630
2018 The small protein MgtS and small RNA MgrR modulate the PitA phosphate symporter to boost intracellular magnesium levels. Molecular microbiology 46 30276893
2004 Rice Pi-ta gene Confers Resistance to the Major Pathotypes of the Rice Blast Fungus in the United States. Phytopathology 44 18943978
2008 Molecular evolution of the Pi-ta gene resistant to rice blast in wild rice (Oryza rufipogon). Genetics 43 18622033
2003 Natural Variation at the Pi-ta Rice Blast Resistance Locus. Phytopathology 39 18944075
2008 Expression of the PitA phosphate/metal transporter of Escherichia coli is responsive to zinc and inorganic phosphate levels. FEMS microbiology letters 38 19054109
2018 KRAB-type zinc-finger proteins PITA and PISA specifically regulate p53-dependent glycolysis and mitochondrial respiration. Cell research 36 29467382
2021 An antibody-based proximity labeling map reveals mechanisms of SARS-CoV-2 inhibition of antiviral immunity. Cell chemical biology 35 34672954
2020 The broad-spectrum rice blast resistance (R) gene Pita2 encodes a novel R protein unique from Pita. Rice (New York, N.Y.) 33 32170462
2017 Architectural protein Pita cooperates with dCTCF in organization of functional boundaries in Bithorax complex. Development (Cambridge, England) 32 28619827
2009 Evolutionary dynamics of the genomic region around the blast resistance gene Pi-ta in AA genome Oryza species. Genetics 32 19822730
2016 Coevolutionary Dynamics of Rice Blast Resistance Gene Pi-ta and Magnaporthe oryzae Avirulence Gene AVR-Pita 1. Phytopathology 30 27070427
2012 Tellurite enters Escherichia coli mainly through the PitA phosphate transporter. MicrobiologyOpen 28 23189244
2008 Haplotype diversity at the Pi-ta locus in cultivated rice and its wild relatives. Phytopathology 28 19000005
2018 Peanut gastrointestinal delivery oral immunotherapy in adolescents: Results of the build-up phase of a randomized, double-blind, placebo-controlled trial (PITA study). Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 27 29665158
2011 Molecular evolution of the rice blast resistance gene Pi-ta in invasive weedy rice in the USA. PloS one 26 22043312
2021 Mechanism and functional role of the interaction between CP190 and the architectural protein Pita in Drosophila melanogaster. Epigenetics & chromatin 24 33752739
1989 Structure, expression and chromosomal localization of Zfp-1, a murine zinc finger protein gene. Nucleic acids research 24 2574853
2023 Antagonistic roles of canonical and Alternative-RPA in disease-associated tandem CAG repeat instability. Cell 23 37827155
2014 Role of an archaeal PitA transporter in the copper and arsenic resistance of Metallosphaera sedula, an extreme thermoacidophile. Journal of bacteriology 22 25092032
2009 Artificial introgression of a large chromosome fragment around the rice blast resistance gene Pi-ta in backcross progeny and several elite rice cultivars. Heredity 20 19623205
2022 Cullin 3 Exon 9 Deletion in Familial Hyperkalemic Hypertension Impairs Cullin3-Ring-E3 Ligase (CRL3) Dynamic Regulation and Cycling. International journal of molecular sciences 18 35563538
2019 The Cys2His2 zinc finger protein Zfp1 regulates sexual reproduction and virulence in Cryptococcus neoformans. Fungal genetics and biology : FG & B 18 30630094
2015 The ActP acetate transporter acts prior to the PitA phosphate carrier in tellurite uptake by Escherichia coli. Microbiological research 18 26211961
2014 Transposon-based high sequence diversity in Avr-Pita alleles increases the potential for pathogenicity of Magnaporthe oryzae populations. Functional & integrative genomics 16 24633351
2013 Molecular diversity in rice blast resistance gene Pi-ta makes it highly effective against dynamic population of Magnaporthe oryzae. Functional & integrative genomics 16 23818197
2009 DNA polymorphism in the blast disease resistance gene Pita of the wild rice Oryza rufipogon and its related species. Genes & genetic systems 16 19556706
2009 The low-affinity phosphate transporter PitA is dispensable for in vitro growth of Mycobacterium smegmatis. BMC microbiology 16 20003273
2024 The unconventional resistance protein PTR recognizes the Magnaporthe oryzae effector AVR-Pita in an allele-specific manner. Nature plants 15 38834685
2022 The in vivo Interaction Landscape of Histones H3.1 and H3.3. Molecular & cellular proteomics : MCP 15 36089195
2005 Morphological irregularities and features of resistance to apoptosis in the dcp-1/pita double mutated egg chambers during Drosophila oogenesis. Cell motility and the cytoskeleton 15 15547953
2009 Fine mapping and association studies of a high-density lipoprotein cholesterol linkage region on chromosome 16 in French-Canadian subjects. European journal of human genetics : EJHG 14 19844255
2009 Determination of Resistance Spectra of the Pi-ta and Pi-k Genes to U.S. Races of Magnaporthe oryzae Causing Rice Blast in a Recombinant Inbred Line Population. Plant disease 13 30764397
2021 Basic pH reversed-phase liquid chromatography (bRPLC) in combination with tip-based strong cation exchange (SCX-Tip), ReST, an efficient approach for large-scale cross-linked peptide analysis. Analytica chimica acta 11 34535262
2020 Up-Regulation of Hsa_circ_0008792 Inhibits Osteosarcoma Cell Invasion and Migration and Promotes Apoptosis by Regulating Hsa-miR-711/ZFP1. OncoTargets and therapy 11 32210583
2018 Functional Interactions Between Major Rice Blast Resistance Genes, Pi-ta and Pi-b, and Minor Blast Resistance Quantitative Trait Loci. Phytopathology 11 29658844
2014 Effectiveness and durability of the rice pi-ta gene in Yunnan province of China. Phytopathology 11 24450460
2022 Pseudomonas aeruginosa Phosphate Transporter PitA (PA4292) Controls Susceptibility to Aminoglycoside Antibiotics by Regulating the Proton Motive Force. Antimicrobial agents and chemotherapy 10 36346250
2015 Ugp and PitA participate in the selection of PHO-constitutive mutants. Journal of bacteriology 8 25645557
2023 PitA Controls the H2- and H3-T6SSs through PhoB in Pseudomonas aeruginosa. Applied and environmental microbiology 7 37184394
2022 Effects of Tasting and Ingredient Information Statement on Acceptability, Elicited Emotions, and Willingness to Purchase: A Case of Pita Chips Containing Edible Cricket Protein. Foods (Basel, Switzerland) 7 35159488
2020 The adhesive PitA pilus protein from the early dental plaque colonizer Streptococcus oralis: expression, purification, crystallization and X-ray diffraction analysis. Acta crystallographica. Section F, Structural biology communications 7 31929180
2008 [Cloning and analyzing of rice blast resistance gene Pi-ta+ allele from Jinghong erect type of common wild rice (Oryza rufipogon Griff) in Yunnan]. Yi chuan = Hereditas 7 18244911
2025 EndoMAP.v1 charts the structural landscape of human early endosome complexes. Nature 6 40437099
2022 Analysis of a rice blast resistance gene Pita-Fuhui2663 and development of selection marker. Scientific reports 5 36050368
2014 Nucleotide diversity of Pita, a major blast resistance gene and identification of its minimal promoter. Gene 5 24905652
2014 Statistical inference of selection and divergence of the rice blast resistance gene Pi-ta. G3 (Bethesda, Md.) 5 25335927
2004 Medicago truncatula Mt-ZFP1 encoding a root enhanced zinc finger protein is regulated by cytokinin, abscisic acid and jasmonate, but not cold. DNA sequence : the journal of DNA sequencing and mapping 5 15346764
2023 The PitA protein contributes to colistin susceptibility in Pseudomonas aeruginosa. PloS one 3 37824582
2022 Fitness benefits play a vital role in the retention of the Pi-ta susceptible alleles. Genetics 3 35143673
2022 Zfp1, a Cys2His2 zinc finger protein is required for meiosis initiation in Tetrahymena thermophila. Cell cycle (Georgetown, Tex.) 3 35293272
2021 In silico molecular and morphological analysis of rice blast resistant gene Pi-ta in Sri Lankan rice germplasm. Journal, genetic engineering & biotechnology 3 34676451
2011 Recognition and suppression of transfected plasmids by protein ZNF511-PRAP1, a potential molecular barrier to transgene expression. Molecular therapy : the journal of the American Society of Gene Therapy 3 21540836
2024 Zinc finger transcription factor ZFP1 is associated with growth, conidiation, osmoregulation, and virulence in the Polygonatum kingianum pathogen Fusarium oxysporum. Scientific reports 2 38992190
2024 ZFP1 is a biomarker related to poor prognosis and immunity in gastric cancer. Scientific reports 2 39261568
2023 Acetylation-Mimic Mutation of TRIM28-Lys304 to Gln Attenuates the Interaction with KRAB-Zinc-Finger Proteins and Affects Gene Expression in Leukemic K562 Cells. International journal of molecular sciences 2 37372979
2023 Genetic and molecular analysis of leaf blast resistance in Tetep derived line RIL4 and its relationship to genes at Pita/Pita2 locus. Scientific reports 2 37907574
2022 The compliance of nutrition claims on pita bread in Lebanon and risk on public health: a cross-sectional study. BMC nutrition 2 35430802
2025 ZAD mediates chromatin binding and insulator activity of Drosophila Pita and can be replaced with the human ZFP276 ZAD-like domain. Epigenetics & chromatin 1 41291933
2024 Exploring Distribution and Evolution of Pi-ta Haplotypes in Rice Landraces across Different Rice Cultivation Regions in Yunnan. Genes 1 39457449
2018 Transcriptome Analysis and Functional Identification of Xa13 and Pi-ta Orthologs in Oryza granulata. The plant genome 1 30512031
2017 Effect of Buttermilk on the Physicochemical, Rheological, and Sensory Qualities of Pan and Pita Bread. International journal of food science 1 29318139
2026 Identification of a Major Rice Blast Quantitative Trait Locus Containing Pita/Pi39(t)/Ptr in U.S. Black Hull Awn Weedy Rice. Phytopathology 0 40952343
2026 Pyramiding Pita, Pigm, Pi2, and Xa23 to Develop Hybrid Rice with Dual Resistance to Rice Blast and Bacterial Blight. Plants (Basel, Switzerland) 0 41600128
2022 Identifying mutations in sd1, Pi54 and Pi-ta, and positively selected genes of TN1, the first semidwarf rice in Green Revolution. Botanical studies 0 35347474
2008 [Genetic diversity of AVR-pita alleles of rice blast fungus Magnaporthe grisea]. Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology 0 19137822