Affinage

XRCC6

DNA repair protein Ku70 · UniProt P12956

Length
609 aa
Mass
69.8 kDa
Annotated
2026-06-11
100 papers in source corpus 49 papers cited in narrative 48 extracted findings
Cross-family judge vs UniProt: tie faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

XRCC6/Ku70 is the DNA end-binding subunit of the Ku70/Ku80 heterodimer that initiates classical non-homologous end joining (NHEJ) and is essential in vivo for double-strand break (DSB) repair, V(D)J recombination, and immunoglobulin class-switch recombination (PMID:9390689, PMID:9362500, PMID:9625768). Heterodimerization with Ku80 must precede DNA binding, with the Ku70 C-terminal domain (aa 254–609) mediating DNA end recognition and a core region (aa 439–609) directing Ku80 assembly (PMID:9362500, PMID:8756676); structural mapping of the heterodimer free, DNA-bound, and DNA-PKcs-bound defines the conformational basis of end recognition (PMID:17159921). Upon irradiation Ku70 accumulates rapidly at breaks through its α/β, DNA-binding, and Ku80-binding domains, and this recruitment is required for downstream loading of XRCC4 and XLF (PMID:21820429); the SAP domain stabilizes Ku70/80 on DNA ends in the absence of DNA-PKcs but is dispensable once the DNA-PK holocomplex forms (PMID:32937838). Ku70 enforces repair-pathway choice by suppressing alternative end-joining in G1-phase cells (PMID:34006647) and is antagonized by the Fanconi anemia effector FANCD2, which diverts breaks toward homologous recombination (PMID:20538911). NHEJ activity is tuned by post-translational modification: acetylation by CBP/PCAF is inhibitory and is reversed by SIRT1, while methylation of Ku70 by SMYD2 promotes Ku70/Ku80/DNA-PKcs recruitment, with loss of SMYD2 driving cytosolic DNA accumulation and cGAS-STING activation (PMID:17334224, PMID:23223795, PMID:37315132); recruitment is further gated by acetylation-controlling interactors SET/TAF-Iβ and SMAR1 (PMID:24305947, PMID:25299772). Beyond repair, Ku70 has cytoplasmic and regulatory functions: it sequesters and deubiquitylates pro-apoptotic Bax in an acetylation-dependent manner controlled by HDAC6 and SIRT6 (PMID:21847364, PMID:19448404, PMID:28238784), acts as a cytosolic DNA/ssDNA sensor that translocates to the cytoplasm to induce STING-dependent IFN-λ1 (PMID:33548066, PMID:28821586), regulates transcription as a BRG1-associated factor for glucocorticoid-receptor activation and as a repressor of Apaf1 (PMID:26055322, PMID:20966962), and is exploited by pathogens as a cell-surface receptor for Rickettsia conorii and a binding partner of HIV-1 integrase and HBoV1 NP1 (PMID:16360032, PMID:21454661, PMID:31690330, PMID:35653410). Ku70 dysfunction is mechanistically linked to neurodegeneration: mutant huntingtin binds Ku70 and impairs NHEJ, and Ku70 re-expression rescues Huntington's disease phenotypes in mice (PMID:20439996, PMID:22096569), while HMGB1/PKCα-mediated phosphorylation of Ku70 at Ser77/78 blocks its DNA binding and drives neuronal cell death in Alzheimer's models (PMID:34635772).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 1997 High

    Established that Ku70 is an essential, non-redundant in vivo component of NHEJ and antigen-receptor gene assembly, defining the pathway it serves.

    Evidence Ku70 knockout mice with IR sensitivity and V(D)J recombination assays, plus class-switch recombination epistasis

    PMID:9390689 PMID:9625768

    Open questions at the time
    • Did not resolve which molecular step of break sealing requires Ku70
    • Cytoplasmic and non-repair roles not addressed
  2. 1997 High

    Defined the domain architecture underlying Ku function, showing heterodimer assembly must precede DNA end binding and mapping the DNA-binding and Ku80-interaction regions.

    Evidence Truncation/chimera mutagenesis, Ku70-/- ES cell complementation, and DNA end-binding assays with purified subunits

    PMID:8756676 PMID:9362500

    Open questions at the time
    • Atomic-resolution conformational changes not resolved at this stage
    • Separability of DNA-PK activation from end-binding only partially mapped
  3. 2006 High

    Provided a structural model of the Ku70/80 heterodimer recognizing DNA and engaging DNA-PKcs, linking domain maps to physical conformational states.

    Evidence Single-particle EM 3D reconstruction of free, DNA-bound, and DNA-PKcs-bound Ku70/80 (with prior IP6 conformational mapping)

    PMID:11953323 PMID:17159921

    Open questions at the time
    • 25 Å resolution insufficient for side-chain detail
    • Functional consequence of IP6-induced conformational change not fully defined
  4. 2011 High

    Resolved the recruitment hierarchy at breaks, showing Ku70 accumulates first and is required to load downstream NHEJ factors.

    Evidence Live-cell imaging of EGFP-Ku70 domain mutants after laser irradiation with XRCC4/XLF/Artemis foci epistasis

    PMID:21820429

    Open questions at the time
    • Why Artemis recruitment is Ku70-independent unexplained
    • Role of individual residues beyond Leu385 not exhaustively tested
  5. 2020 High

    Clarified how the Ku70 SAP domain and DNA-PK assembly state govern stable retention on DNA ends.

    Evidence FRET and SPR with SAP and Ku80 C-terminal deletions in DNA-PK complex formation assays

    PMID:32937838

    Open questions at the time
    • In vivo relevance of holocomplex stability differences not tested
    • Single biophysical system
  6. 2021 High

    Showed Ku70 actively enforces repair-pathway choice by suppressing alternative end-joining in G1, distinguishing its barrier function from canonical ligation.

    Evidence Genetic epistasis in Lig4-deficient and Ku70/Lig4 double-deficient G1 progenitor B cells across RAG-, Cas9-, and ZFN-induced breaks

    PMID:34006647

    Open questions at the time
    • Mechanism by which Ku occludes A-EJ factors not defined
    • Cell-cycle phase specificity not mechanistically explained
  7. 2030 High

    Mapped how Fanconi anemia signaling counteracts Ku70 to channel breaks toward homologous recombination.

    Evidence FANCC/Ku70 double-knockout epistasis with ICL sensitivity, HR frequency, and in vitro FANCD2/Ku70 biochemistry

    PMID:20538911

    Open questions at the time
    • Molecular nature of FANCD2 end-modification not fully defined
    • Generalizability beyond ICL-induced breaks unclear
  8. 2023 Medium

    Established acetylation, deacetylation, and methylation as the regulatory code controlling Ku70 repair activity and its recruitment to breaks.

    Evidence SIRT1/CBP acetylation-repair coupling, SET/TAF-Iβ and SMAR1 acetylation gating, and SMYD2 methylation site mapping with NHEJ and cGAS-STING readouts

    PMID:17334224 PMID:23223795 PMID:24305947 PMID:25299772 PMID:37315132

    Open questions at the time
    • Interplay/ordering of competing modifications not resolved
    • Several modifier relationships rest on single-lab evidence
  9. 2017 Medium

    Defined Ku70's cytoplasmic anti-apoptotic function through acetylation-dependent Bax sequestration and deubiquitylation, governed by HDAC6 and SIRT6.

    Evidence HDAC6/SIRT6 co-IP and knockdown, K542 mutagenesis, Bax deubiquitylation assay, and mitochondrial translocation/apoptosis readouts

    PMID:19448404 PMID:21847364 PMID:22745744 PMID:28238784

    Open questions at the time
    • Catalytic basis of Ku70 DUB activity on Bax not structurally defined
    • Balance between Bax sequestration and stabilization context-dependent and unresolved
  10. 2021 Medium

    Identified a non-repair role for Ku70 as a cytosolic DNA/ssDNA sensor driving STING-dependent type III interferon induction.

    Evidence Leptomycin B nuclear-export block, IFN-λ1 reporter, ssDNA90 binding, and viral infection (HSV-1, HTLV-1) with STING association

    PMID:28821586 PMID:33548066

    Open questions at the time
    • Why Ku70 selectively induces IFN-λ1 but not IFN-α/β unexplained
    • Direct STING-engagement mechanism not biochemically resolved
  11. 2024 Medium

    Extended Ku70 into transcriptional and tumor-suppressive control via BRG1-dependent nuclear receptor activation, Apaf1 repression, and YAP-TEAD4 competition.

    Evidence Co-IP/ChIP for BRG1 and Apaf1 promoters, and Ku70-YAP competition with in vivo tumor and ubiquitination assays

    PMID:20966962 PMID:26055322 PMID:38862269

    Open questions at the time
    • Whether these transcriptional roles require DNA-end binding unclear
    • Single-lab evidence for each axis
  12. 2021 High

    Connected Ku70 dysfunction to neurodegeneration, showing impaired Ku70-mediated NHEJ contributes to Huntington's and Alzheimer's pathology.

    Evidence Mutant huntingtin co-IP and Ku70 rescue in R6/2 mice; HMGB1/PKCα phosphorylation of Ku70 Ser77/78 with anti-HMGB1 rescue in AD mice

    PMID:20439996 PMID:22096569 PMID:34635772

    Open questions at the time
    • Causal contribution of Ku70 loss versus broader DSB burden not isolated
    • No reported XRCC6 Mendelian disease mutation in the corpus

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the many regulatory inputs (acetylation, methylation, phosphorylation, partner binding) are integrated to switch Ku70 between nuclear repair, cytoplasmic apoptotic control, immune sensing, and transcription remains unresolved.
  • No unified model linking modification state to subcellular partitioning
  • Many partner interactions validated only by single-lab co-IP without reciprocal structural validation

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003677 DNA binding 5 GO:0003723 RNA binding 2 GO:0060090 molecular adaptor activity 2 GO:0140096 catalytic activity, acting on a protein 2 GO:0140110 transcription regulator activity 2 GO:0140299 molecular sensor activity 2 GO:0001618 virus receptor activity 1
Localization
GO:0005634 nucleus 3 GO:0005694 chromosome 3 GO:0005829 cytosol 3 GO:0005886 plasma membrane 1
Pathway
R-HSA-73894 DNA Repair 4 R-HSA-168256 Immune System 3 R-HSA-5357801 Programmed Cell Death 3 R-HSA-1643685 Disease 2 R-HSA-74160 Gene expression (Transcription) 2
Partners
BAXFANCD2FOXO4HDAC6PRKDCSIRT1SMARCA4XRCC5
Complex memberships
DNA-PK holocomplexKu70/Ku80 heterodimer

Evidence

Reading pass · 48 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 Ku70 forms a heterodimer with Ku80 that is required for DNA double-strand break (DSB) repair and V(D)J recombination. Loss-of-function (Ku70-/- mice) showed radiation sensitivity, premature senescence, and severe impairment of V(D)J coding and signal sequence joining, establishing Ku70 as an essential component of the NHEJ pathway in vivo. Ku70 knockout mouse model; cellular IR sensitivity assays; V(D)J recombination assays Immunity High 9390689
1997 Ku70 requires heterodimerization with Ku80 and its C-terminal DNA-binding domain (aa 254–609) for DSB repair and DNA end-binding. A core region (aa 439–609) mediates Ku80 heterodimerization. Ku70's roles in DNA-PK activation and IR repair are separable by mutagenesis. Truncation and chimera mutagenesis of Ku70; complementation of Ku70-/- ES cells; IR sensitivity assay; DNA end-binding assay; DNA-PK activity assay The EMBO journal High 9362500
1996 The C-terminal 20 kDa of Ku70 and C-terminal 32 kDa of Ku86 mediate subunit–subunit interaction; the C-terminal 40 kDa of Ku70 (aa 254–609) is required for DNA end-binding. Full-length individual subunits are inactive for DNA binding; heterodimer assembly must precede DNA binding. Genetic and biochemical domain-deletion analysis; DNA end-binding assays with purified proteins Molecular and cellular biology High 8756676
1998 Ku70 is required for immunoglobulin heavy chain class switch recombination (CSR). K70T/HL B cells induced with CSR-stimulating agents showed intact germline CH transcription but completely failed to undergo CSR and underwent cell death, placing Ku70 downstream of CSR signaling but essential for the DNA rearrangement step. Genetic epistasis using Ku70-/- mice carrying rearranged Ig transgenes; in vitro CSR assays; serum Ig measurement The Journal of experimental medicine High 9625768
2002 Inositol hexakisphosphate (IP6) binds specifically to the Ku70/80 heterodimer (not to DNA-PKcs alone), and IP6 binding alters the conformation of Ku70/80 as detected by proteolysis mapping. The yeast Ku70/80 homolog does not bind IP6, indicating this is a mammalian-specific function. Proteolysis mapping of Ku70/80 with and without IP6; binding competition assays; comparison with yeast Ku homologs The EMBO journal High 11953323
2006 Full-length 3D structure of human Ku70/Ku80 heterodimer resolved at 25 Å by single-particle electron microscopy, alone and in complex with DNA. C-terminal domains of both subunits were mapped; conformational changes upon DNA and DNA-PKcs binding were defined, providing a structural model for DNA recognition during NHEJ. Single-particle electron microscopy; 3D reconstruction; structural comparison of Ku70/80 free, DNA-bound, and DNA-PKcs-bound states EMBO reports High 17159921
2005 Ku70 is a mammalian cell-surface receptor for Rickettsia conorii internalization. Ku70 is recruited to bacterial entry sites; cholesterol-enriched microdomains containing Ku70 are required for invasion. R. conorii infection stimulates Ku70 ubiquitination, and the ubiquitin ligase c-Cbl is recruited to entry foci and mediates Ku70 ubiquitination. The rickettsial surface protein rOmpB was identified as the Ku70 ligand by affinity chromatography. siRNA knockdown; immunofluorescence/confocal colocalization; affinity chromatography; co-immunoprecipitation; bacterial invasion assay Cell High 16360032
2007 SIRT1 physically complexes with Ku70, deacetylates it, and thereby enhances DNA repair capacity after radiation. A catalytically inactive SIRT1 dominant-negative mutant failed to deacetylate Ku70 or enhance repair, establishing a writer/eraser relationship between SIRT1 and Ku70 acetylation in DNA repair. Co-immunoprecipitation; SIRT1 overexpression and siRNA knockdown; DNA strand-break repair assay; dominant-negative SIRT1 mutagenesis Experimental & molecular medicine Medium 17334224
2011 HDAC6 binds Ku70 and Bax in the cytoplasm of neuroblastoma cells and maintains Ku70 in a deacetylated state. Knockdown of HDAC6 or use of an HDAC6-specific inhibitor triggers Ku70 acetylation, releases Bax from Ku70, and induces Bax-dependent apoptosis, identifying HDAC6 as a key deacetylase (eraser) for cytoplasmic Ku70. Co-immunoprecipitation; HDAC6 siRNA knockdown; HDAC6-specific inhibitor (Tubacin); apoptosis assays Neoplasia High 21847364
2012 Ku70 acetylation (mediated by CBP/PCAF) disrupts its interaction with FLIP, triggering FLIP polyubiquitination and proteasomal degradation. HDAC inhibitor (SAHA/Vorinostat) enhances Ku70 acetylation via HDAC6 inhibition to destabilize FLIP and induce caspase-8-dependent apoptosis in colorectal cancer models. Co-immunoprecipitation; HDAC inhibitor treatment; ubiquitination assays; in vitro and in vivo colorectal cancer models; caspase activation assays Cell death and differentiation High 22322857
2005 Human Ku70/80 interacts directly with hTR, the RNA component of human telomerase, specifically with a 47 nt region at the 3' end of hTR. This interaction is independent of hTERT, as shown by immunoprecipitation/RT-PCR in hTERT-deficient cell lines, suggesting a conserved role for Ku in telomere maintenance via telomerase RNA interaction. In vitro binding assay; immunoprecipitation/RT-PCR in hTERT-deficient cell lines; mapping of interaction domain on hTR Nucleic acids research Medium 15824061
2000 Ku70 interacts with heterochromatin protein 1alpha (HP1alpha) via its Leu-Ser repeat domain (aa 200–385), and HP1alpha's chromo shadow domain mediates the interaction. This was confirmed by GST pull-down with recombinant proteins and co-immunoprecipitation in HeLa cells. The interaction is pH-sensitive and Ku80 does not interact with HP1alpha directly. Yeast two-hybrid; GST pull-down with recombinant proteins; co-immunoprecipitation in HeLa cells; confocal colocalization The Journal of biological chemistry Medium 11112778
2010 In the Fanconi anemia pathway, FANCD2 (a downstream FA effector) antagonizes Ku70 activity by modifying free DNA ends, thereby diverting DSB repair from NHEJ toward homologous recombination. Disruption of both FANCC and Ku70 suppresses ICL sensitivity, diminishes chromosome breaks, and reverses defective HR, establishing a genetic epistasis where the FA pathway acts upstream of Ku70 to channel repair pathway choice. Genetic epistasis (FANCC/Ku70 double knockout); ICL sensitivity assays; HR frequency measurement; in vitro biochemical assay with purified FANCD2 and Ku70 Science High 20538911
2010 Mutant huntingtin (Htt) directly interacts with Ku70 and impairs DNA-PK function in NHEJ, causing accumulation of DSBs in neurons. Exogenous Ku70 expression rescues abnormal behavior and pathological phenotypes in R6/2 HD mice, establishing Ku70 as a critical mediator of DNA repair dysfunction in Huntington's disease pathology. Co-immunoprecipitation; DNA-PK activity assay; γH2AX foci analysis; R6/2 mouse rescue with Ku70 overexpression; behavioral assays The Journal of cell biology High 20439996 22096569
2007 Ku70/80 modulates ATM-dependent ATR activation during the DSB damage response. In Ku-deficient cells, p53 Ser18 phosphorylation persists via ATM-independent ATR activity at late time points after IR, a pathway not observed in wild-type cells, revealing a novel role for Ku70/80 in suppressing aberrant ATR activation. ATM/Ku70 double-null cell line generation; p53 phosphorylation analysis; ATM and ATR inhibitors; irradiation experiments The Journal of biological chemistry Medium 17272272
2000 A nuclear CLU/XIP8 protein, induced by low-dose ionizing radiation, co-immunoprecipitates and co-localizes with Ku70/Ku80 in human breast cancer cells. Overexpression of nuclear CLU/XIP8 or its minimal Ku70-binding domain (120 aa C-terminus) reduces cell growth and increases G1 arrest and cell death, identifying clusterin as a Ku70-binding partner that signals cell death. Yeast two-hybrid; co-immunoprecipitation; confocal colocalization; overexpression of CLU/XIP8 and truncation mutants; colony formation assay PNAS Medium 10823943
2006 Granzyme A cleaves Ku70 after Arg301, disrupting Ku complex binding to DNA, thereby facilitating GzmA-mediated caspase-independent cell death. Silencing Ku70 by RNAi increases GzmA-induced DNA damage and cell death, while Ku70 overexpression has the opposite effect, demonstrating Ku70 has antiapoptotic functions beyond DSBR and Bax sequestration. In vitro cleavage assay; Ku70 siRNA knockdown; Ku70 overexpression; CTL/perforin cell death assay; DNA damage quantification EMBO reports High 16440001
2002 Ku70 can translocate to the nucleus independently of heterodimerization with Ku80, as shown by nuclear localization of Ku70 mutants lacking Ku80-binding ability and by Ku70 expression in Ku80-deficient hamster xrs-6 cells. Ku70 nuclear localization is mediated by its NLS and does not require DNA-PK autophosphorylation sites. Site-directed mutagenesis of Ku70 NLS and Ku80-binding domains; transfection into Ku80-deficient cells; immunofluorescence localization Biochemical and biophysical research communications Medium 11027597
2011 Ku70 interacts with FOXO4 via a direct biochemical interaction (Ku70 necessary and sufficient), and sequesters FOXO4 in the nucleus, inhibiting FOXO4-mediated p27(kip1) transcription and cell cycle arrest by >40%. Low oxidative stress increases Ku70-FOXO4 interaction stoichiometry, while higher stress causes dissociation, suggesting a dose-dependent stress-response role for Ku70. Tandem-affinity purification/mass spectrometry; co-immunoprecipitation; luciferase reporter assay; flow cytometry; Ku70-/- ES cells; immunofluorescence FASEB journal High 20570964
2011 Ku70 interacts directly with HIV-1 integrase (IN) via the N-terminal part of Ku70 (aa 1–430), protecting IN from Lys48-linked polyubiquitination and proteasomal degradation. Ku70 can deubiquitinate IN through their interaction. This interaction is independent of Ku70/80 heterodimerization, and Ku70 is incorporated into progeny virus in an IN-dependent manner; Ku70 knockdown disrupts HIV-1 replication. Co-immunoprecipitation; mutagenesis mapping; ubiquitination assays; Ku70 siRNA knockdown; HIV infectivity assays The Journal of biological chemistry High 21454661
2010 MSH6 is a novel Ku70-interacting protein; the Ku70-MSH6 association is enhanced by DSB-inducing agents (NCS, IR). MSH6-deficient cells accumulate persistent DSBs and display impaired NHEJ, which is rescued by MSH6 overexpression, linking the mismatch repair protein MSH6 to NHEJ via Ku70. Yeast two-hybrid; co-immunoprecipitation; γH2AX foci assay; comet assay; NHEJ assay; clonogenic survival Nucleic acids research Medium 21075794
2007 Par3, a cell polarity protein, interacts with Ku70 and Ku80 in the nucleus; this interaction is enhanced by gamma-irradiation. Par3 regulates DNA-PK activity (up- and downregulated by overexpression and knockdown, respectively), and Par3-knockdown cells are defective in NHEJ, random integration, and DSB repair, and are radiosensitive. In vitro binding assay; LC-MS/MS; co-immunoprecipitation; DNA-PK activity assay; NHEJ reporter assay; IR sensitivity assay Cell research Medium 17287830
2008 Ku70 and Ku80 interact with full-length RAG1 as demonstrated by co-immunoprecipitation, providing a biochemical link between the RAG cleavage machinery and the NHEJ repair pathway during V(D)J recombination. Co-immunoprecipitation with full-length RAG1 and Ku70/80 Nucleic acids research Low 18281312
2004 Ku70/80 and DNA-PKcs modulate RAG-mediated V(D)J cleavage, preferentially inhibiting 12/12 and 23/23 cleavage to increase 12/23 rule specificity. This indicates DNA repair factors are present upstream of cleavage events, not only recruited downstream for repair. Protein fractionation; biochemical cleavage assays with purified Ku70/80 and DNA-PKcs; 12/23 rule reporter assays The Journal of biological chemistry Medium 15123719
2013 RECQ1 helicase directly interacts with Ku70/80; depletion of RECQ1 reduces end-joining in cell-free extracts. In vitro, RECQ1 binds and unwinds Ku70/80-bound partial duplex DNA, and linear DNA is co-bound by both RECQ1 and Ku70/80, with DNA binding by Ku70/80 modulated by RECQ1. Direct protein interaction assays; cell-free end-joining assay; helicase unwinding assay with Ku70/80-bound substrate; co-binding assay PloS one Medium 23650516
2005 DNA-PK-mediated phosphorylation of Ku70 at serine 6 (and Ku80 at S577, S580, T715) occurs in vitro, but these phosphorylation events are not required for NHEJ in vivo: Ku70/80 with S/T→A mutations at these sites fully complemented radiation sensitivity of Ku-negative cells. In vivo phosphorylation at these sites is regulated by a PP2A-like phosphatase and a staurosporine-sensitive kinase, not DNA-PK. Mass spectrometry; phosphospecific antibodies; in vitro kinase assays; S/T→A mutagenesis; complementation of Ku-negative cells with mutant Ku70/80; IR sensitivity assay; pharmacological inhibitors DNA repair High 15941674
2012 Ku70/80 binds the Apaf1 promoter repressor element and downregulates Apaf1 transcription. This binding is dynamically modulated upon DNA damage: Ku70/80 initially represses Apaf1 after damage, then dissociates allowing Apaf1 upregulation and apoptosis. This defines a transcriptional regulatory function for Ku70/80 at the apoptosome pathway. Apaf1 promoter mutant analysis; chromatin immunoprecipitation; reporter assays; Ku70/80 knockdown with Apaf1 expression measurement Cell death and differentiation Medium 20966962
2013 SET/TAF-Iβ (an INHAT subunit) interacts with Ku70/80, inhibiting CBP- and PCAF-mediated acetylation of Ku70 in an INHAT domain-dependent manner. DNA damage by UV disrupts this interaction, releasing Ku70/80 for recruitment to DSB sites. Overexpressed SET/TAF-Iβ inhibits Ku70/80 recruitment to DNA damage foci. Co-immunoprecipitation; in vitro acetylation assay with CBP/PCAF; SET/TAF-Iβ overexpression; immunofluorescence of Ku70/80 at damage foci Cellular and molecular life sciences Medium 24305947
2015 Ku70 (XRCC6) directly associates with the HSA/BRK domains of BRG1 (SWI/SNF ATPase). Ku70/86 and components of the TOP2β/PARP1 complex are required for glucocorticoid receptor (GR)-mediated, BRG1-dependent transcriptional activation from endogenous promoters. GR/BRG1-dependent, TOP2β-mediated transient DNA DSBs are required for efficient GR-stimulated transcription. Co-immunoprecipitation; chromatin immunoprecipitation; transcriptional activation assays; BRG1 mutant analysis; Ku70/86 knockdown Molecular and cellular biology Medium 26055322
2017 SIRT6 interacts with Ku70 and deacetylates it at lysine K542. Deacetylation of Ku70 by SIRT6 stabilizes its interaction with Bax, preventing Bax mitochondrial translocation and apoptosis. A K542Q acetylation-mimicking mutation abolishes Ku70-Bax association and reverses SIRT6-mediated apoptosis suppression in hepatocellular carcinoma cells. Co-immunoprecipitation; SIRT6 knockdown; site-directed mutagenesis (K542Q); mitochondrial fractionation; apoptosis assays Biochemical and biophysical research communications Medium 28238784
2023 SMYD2 methyltransferase methylates Ku70 at lysines 74, 516, and 539 in response to DNA damage, increasing recruitment of the Ku70/Ku80/DNA-PKcs complex. SMYD2 knockdown or inhibition impairs NHEJ, causes cytosolic DNA accumulation, and activates the cGAS-STING pathway, triggering anti-tumor CD8+ T-cell responses. Mass spectrometry identification of methylation sites; SMYD2 knockdown/inhibitor (AZ505); ChIP for Ku70 at damage sites; NHEJ assay; cGAS-STING activation measurement; in vivo tumor immunology Science advances High 37315132
2021 Cytoplasmic translocation of Ku70 (via nuclear export, inhibited by leptomycin B) is required for cytosolic DNA sensing and induction of IFN-λ1 (but not IFN-α or IFN-β) via a STING-dependent signaling pathway. Enhanced cellular acetylation promotes Ku70 cytoplasmic accumulation and IFN-λ1 induction. HSV-1 infection triggers Ku70 cytoplasmic translocation and IFN-λ1 in a strain-dependent manner. Nuclear export inhibitor (leptomycin B); IFN-λ1 reporter assay; DNA transfection; HSV-1 infection; subcellular fractionation; kinetic analysis Immunology Medium 33548066
2017 Ku70 senses HTLV-1 reverse transcription intermediate ssDNA90, is induced by HTLV-1 infection, and associates with STING to mediate innate immune responses. Ku70 overexpression inhibited HTLV-1 protein expression while knockdown promoted it. Ku70 interacts with HTLV-1 ssDNA90 and promotes ssDNA-triggered innate immune signaling. HTLV-1 infection; Ku70 overexpression/knockdown; STING co-association; innate immune cytokine measurement; ssDNA90 binding assay Journal of immunology Medium 28821586
2011 Ku70 accumulates at DSBs immediately after irradiation in living epithelial cells. Three domains—α/β, DNA-binding, and Ku80-binding—but not the SAP domain, are required for early accumulation at DSBs. Leucine 385 in the Ku80-binding domain is required for DSB localization. Accumulation of XRCC4 and XLF at DSBs depends on Ku70 presence, but Artemis accumulation does not. EGFP-Ku70 domain deletion mutants; live-cell imaging after laser irradiation; domain truncation and point mutagenesis; XRCC4/XLF/Artemis foci analysis in Ku70-depleted cells Experimental cell research High 21820429
2021 Ku suppresses alternative end-joining (A-EJ) in G1-arrested progenitor B cells. In Lig4-deficient G1-arrested progenitor B cells, deletion of Ku70 increases DSB rejoining and translocation, demonstrating Ku70 actively blocks A-EJ of RAG-, Cas9-, and zinc-finger nuclease-generated DSBs in G1 phase. Genetic epistasis using Lig4-deficient and Ku70/Lig4 double-deficient G1-arrested progenitor B cell lines; translocation assays; V(D)J recombination assays; Cas9 and ZFN-generated DSB rejoining PNAS High 34006647
2019 Ku70 directly interacts with HIV-1 integrase via its C-terminus (IN aa 230–288 binding to Ku70 aa 1–430); this interaction recruits DNA-PK to the site of HIV-1 post-integrational DNA gap repair. Pseudoviruses with Ku70-binding-defective IN are defective in gap repair, and NHEJ pathway disruption by Ku70/Ku80/DNA-PKcs KO or inhibition reduces HIV-1 infectivity in a Ku70-binding-dependent manner. HIV pseudovirus with mutant IN; CRISPR/Cas9 KO of Ku70/Ku80/DNA-PKcs; qPCR gap-repair assay; HIV infectivity measurement in primary PBMCs and cell lines Retrovirology High 31690330
2012 CREB-binding protein (CBP) acetylates Ku70 in the nucleus after ionizing radiation in neuroblastoma cells, and this nuclear acetylation plays an inhibitory role in DNA repair. Cytoplasmic Ku70 redistributes to the nucleus following irradiation. Depleting CBP reduces Ku70 acetylation and enhances DNA repair activity. CBP depletion; IR treatment; Ku70 acetylation measurement; DNA repair assay; nuclear/cytoplasmic fractionation Molecular cancer research Medium 23223795
2016 LSD1 and SIRT1 competitively bind the core domain of Ku70 on DNA damage foci. The Ku70 SAP/C-terminal motif suppresses LSD1 binding. SIRT1 promotes NHEJ repair and mutation acquisition while LSD1 has the opposite effect; SIRT1 maintains H4K16 acetylation and open chromatin for repair. SAP-deleted Ku70 compromises BCR-ABL mutation acquisition. Co-immunoprecipitation; chromatin immunoprecipitation at global and locus-specific (ABL) levels; Ku70 domain mutagenesis; BCR-ABL mutation acquisition assay Oncotarget Medium 27384990
2024 Ku70 dynamically competes with TEAD4 for binding to YAP, limiting YAP transcriptional activity. Loss of Ku70 enhances YAP-TEAD4 interaction, which drives SMURF2-mediated ubiquitin-proteasome degradation of PARP1, increasing genome instability and tumorigenesis in colon cancer and HCC. Co-immunoprecipitation; Ku70 depletion; in vivo tumor models; ubiquitination assay; patient HCC sample analysis Cancer research Medium 38862269
2022 Ku70 (XRCC6) directly interacts with the HBoV1 non-structural protein NP1, with a high-affinity KD of ~95 nM. The Ku70 binding domain on NP1 maps to Ku70 aa 266–439. Dominant-negative disruption of Ku70-NP1 interaction impairs HBoV1 DNA replication both in vitro and in HAE-ALI cultures. Affinity purification; direct binding assay (KD measurement); domain mapping; dominant-negative disruption; viral DNA replication assay in HAE-ALI cultures PLoS pathogens Medium 35653410
2021 HMGB1 extracellular signaling activates PKCα via TLR4, which phosphorylates Ku70 at Ser77/78. This phosphorylation prevents Ku70-DNA interaction and blocks Ku70 accumulation at DSB foci, impairing DNA repair and causing transcriptional repression-induced atypical cell death (TRIAD) in Alzheimer's disease neurons. Anti-HMGB1 antibody treatment reduces neuronal DSBs and ameliorates cognitive symptoms in AD mice. Phosphoproteome analysis of AD postmortem brains; site-specific phosphomutant analysis; Ku70-DNA binding assay; foci immunofluorescence; anti-HMGB1 antibody treatment in AD mouse model; behavioral assays Communications biology High 34635772
2009 Ku70 possesses a deubiquitylase (DUB) enzymatic activity on Bax, removing ubiquitin from Bax to prevent its proteasomal degradation. This dual role (sequestration and deubiquitylation of Bax) gives Ku70 both anti-apoptotic (preventing mitochondrial Bax translocation) and pro-apoptotic (blocking Bax degradation) functions. Deubiquitylation assay with Ku70 and ubiquitinated Bax; co-immunoprecipitation Cell cycle Medium 19448404
2010 The 55 kDa isoform of CDK9 (but not the 42 kDa isoform) specifically associates with Ku70. shRNA depletion of the 55K CDK9 induces apoptosis and DSBs, and rescue with an shRNA-resistant 55K CDK9 reverses these phenotypes, suggesting the 55K CDK9 may function in DSB repair through Ku70. Co-immunoprecipitation (isoform-specific); shRNA knockdown of 55K CDK9; apoptosis and DSB quantification; rescue experiment Biochemical and biophysical research communications Low 20493174
2014 SMAR1 interacts with Ku70 and coordinates with HDAC6 to maintain Ku70 in a deacetylated state. SMAR1 knockdown results in enhanced Ku70 acetylation, impaired chromatin recruitment of Ku70, and increased apoptosis. IR induces SMAR1 redistribution as nuclear foci via ATM-mediated phosphorylation at Ser370. Deacetylated Ku70 (facilitated by SMAR1) maintains its interaction with Bax to prevent mitochondrial translocation. Co-immunoprecipitation; SMAR1 knockdown; Ku70 acetylation assay; chromatin fractionation; immunofluorescence foci analysis; phosphorylation site mapping Cell death & disease Medium 25299772
2016 EAF2 (and EAF1) are required for the recruitment and retention of Ku70/Ku80 to DNA damage sites and play a functional role in NHEJ. EAF2 knockdown sensitizes prostate cancer cells and mouse prostate to DNA damage, and blocks androgen repression of DNA damage markers. EAF2 knockdown; γH2AX marker analysis; Ku70/Ku80 foci recruitment assay; NHEJ reporter assay; EAF2 KO mouse prostate irradiation Oncogene Medium 27721405
2012 Caveolin-1 is a novel Ku70-binding protein; the scaffolding domain of caveolin-1 (aa 82–101) binds the caveolin-binding domain (CBD) of Ku70 (aa 471–478). Caveolin-1 binding to Ku70 inhibits chemotherapeutic drug-induced Bax release from Ku70 and prevents Bax mitochondrial translocation and apoptosis. A Ku70 mutant unable to bind caveolin-1 (Ku70 Φ→A) does not suppress Ku70/Bax dissociation. In vitro binding assay (domain mapping); co-immunoprecipitation; Ku70 point mutagenesis; caveolin-1 knockdown; apoptosis/Bax assay PloS one Medium 22745744
2016 Ku70 binds double-stranded DNA and hairpin RNA through two distinct binding sites: the C-terminal region including the SAP domain recognizes dsDNA, while a separate site (aa 251–438) mediates hairpin RNA binding. Unlike the Ku heterodimer, monomeric Ku70 can interact with closed circular DNA. Recombinant Ku70 truncation mutants expressed in E. coli; nucleic acid binding assays with diverse substrates; surface plasmon resonance or equivalent binding assays Biochimie Medium 27825805
2020 The Ku70 C-terminal SAP domain is required for stable association of Ku70/80 to DNA ends in the absence of DNA-PKcs, but this requirement is abrogated in the DNA-PK holocomplex. Ku80 C-terminal extension contributes to DNA-PK complex stability but is not absolutely required for its formation. FRET assay with ECFP-Ku70/EYFP-Ku80 confirmed that DNA-PK phosphorylation status influences holocomplex stability. FRET assay with fluorescently tagged Ku70/80; surface plasmon resonance; SAP domain deletion mutagenesis; Ku80 C-terminal extension deletion; DNA-PK complex formation assays International journal of molecular sciences High 32937838

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1997 Growth retardation and leaky SCID phenotype of Ku70-deficient mice. Immunity 364 9390689
2007 SIRT1 promotes DNA repair activity and deacetylation of Ku70. Experimental & molecular medicine 290 17334224
2000 Nuclear clusterin/XIP8, an x-ray-induced Ku70-binding protein that signals cell death. Proceedings of the National Academy of Sciences of the United States of America 242 10823943
1998 Ku70 is required for late B cell development and immunoglobulin heavy chain class switching. The Journal of experimental medicine 227 9625768
2010 Ku70 corrupts DNA repair in the absence of the Fanconi anemia pathway. Science (New York, N.Y.) 201 20538911
1998 Ku70: a candidate tumor suppressor gene for murine T cell lymphoma. Molecular cell 193 9702186
2006 Enhanced gene targeting frequency in ku70 and ku80 disruption mutants of Aspergillus sojae and Aspergillus oryzae. Molecular genetics and genomics : MGG 142 16470383
2005 Ku70, a component of DNA-dependent protein kinase, is a mammalian receptor for Rickettsia conorii. Cell 139 16360032
1997 Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions. The EMBO journal 139 9362500
2010 Mutant huntingtin impairs Ku70-mediated DNA repair. The Journal of cell biology 118 20439996
2019 Fusobacterium nucleatum Caused DNA Damage and Promoted Cell Proliferation by the Ku70/p53 Pathway in Oral Cancer Cells. DNA and cell biology 112 31765243
2006 Structural model of full-length human Ku70-Ku80 heterodimer and its recognition of DNA and DNA-PKcs. EMBO reports 105 17159921
1996 Protein-protein and protein-DNA interaction regions within the DNA end-binding protein Ku70-Ku86. Molecular and cellular biology 104 8756676
2012 The dynamics of Ku70/80 and DNA-PKcs at DSBs induced by ionizing radiation is dependent on the complexity of damage. Nucleic acids research 99 23012265
2002 Dimerization, translocation and localization of Ku70 and Ku80 proteins. Journal of radiation research 98 12518983
2002 Specific interaction of IP6 with human Ku70/80, the DNA-binding subunit of DNA-PK. The EMBO journal 91 11953323
2011 HDAC6 deacetylates Ku70 and regulates Ku70-Bax binding in neuroblastoma. Neoplasia (New York, N.Y.) 90 21847364
1999 Isolation of Ku70-binding proteins (KUBs). Nucleic acids research 89 10219089
2012 Identification of an acetylation-dependant Ku70/FLIP complex that regulates FLIP expression and HDAC inhibitor-induced apoptosis. Cell death and differentiation 85 22322857
2005 Human Ku70/80 interacts directly with hTR, the RNA component of human telomerase. Nucleic acids research 85 15824061
2002 Expression of Ku70 and Ku80 mediated by NF-kappa B and cyclooxygenase-2 is related to proliferation of human gastric cancer cells. The Journal of biological chemistry 85 12324457
2001 Tumor specific modulation of KU70/80 DNA binding activity in breast and bladder human tumor biopsies. Oncogene 83 11314007
2007 Bax-inhibiting peptides derived from Ku70 and cell-penetrating pentapeptides. Biochemical Society transactions 81 17635151
2005 DNA-PK-dependent phosphorylation of Ku70/80 is not required for non-homologous end joining. DNA repair 77 15941674
2021 The Multifaceted Roles of Ku70/80. International journal of molecular sciences 76 33923616
2011 Telomere maintenance genes SIRT1 and XRCC6 impact age-related decline in telomere length but only SIRT1 is associated with human longevity. Biogerontology 74 21972126
2015 Glucocorticoid Receptor Transcriptional Activation via the BRG1-Dependent Recruitment of TOP2β and Ku70/86. Molecular and cellular biology 70 26055322
2016 A novel small molecule inhibitor of the DNA repair protein Ku70/80. DNA repair 65 27130816
2008 Ku70, an essential gene, modulates the frequency of rAAV-mediated gene targeting in human somatic cells. Proceedings of the National Academy of Sciences of the United States of America 64 18562296
2000 Human Ku70 interacts with heterochromatin protein 1alpha. The Journal of biological chemistry 64 11112778
2022 CIRKIL Exacerbates Cardiac Ischemia/Reperfusion Injury by Interacting With Ku70. Circulation research 62 35105170
2019 Apoptosis Induction byHistone Deacetylase Inhibitors in Cancer Cells: Role of Ku70. International journal of molecular sciences 58 30935057
2011 Host protein Ku70 binds and protects HIV-1 integrase from proteasomal degradation and is required for HIV replication. The Journal of biological chemistry 56 21454661
2012 Suppression of Ku70/80 or Lig4 leads to decreased stable transformation and enhanced homologous recombination in rice. The New phytologist 54 23050791
2013 Clinicopathological significance of KU70/KU80, a key DNA damage repair protein in breast cancer. Breast cancer research and treatment 52 23624778
2010 Mismatch-repair protein MSH6 is associated with Ku70 and regulates DNA double-strand break repair. Nucleic acids research 52 21075794
2018 MicroRNA-379-5p is associate with biochemical premature ovarian insufficiency through PARP1 and XRCC6. Cell death & disease 49 29367615
2006 Granzyme A, which causes single-stranded DNA damage, targets the double-strand break repair protein Ku70. EMBO reports 49 16440001
2003 Function of DNA-protein kinase catalytic subunit during the early meiotic prophase without Ku70 and Ku86. Biology of reproduction 47 12604618
2013 Human RECQ1 interacts with Ku70/80 and modulates DNA end-joining of double-strand breaks. PloS one 46 23650516
2008 Evidence for Ku70/Ku80 association with full-length RAG1. Nucleic acids research 45 18281312
2007 Expression of the Ku70 subunit (XRCC6) and protection from low dose ionizing radiation during zebrafish embryogenesis. Neuroscience letters 42 17630212
2013 Repairing DNA damage by XRCC6/KU70 reverses TLR4-deficiency-worsened HCC development via restoring senescence and autophagic flux. Autophagy 41 23518600
2007 Cell polarity protein Par3 complexes with DNA-PK via Ku70 and regulates DNA double-strand break repair. Cell research 41 17287830
2010 55K isoform of CDK9 associates with Ku70 and is involved in DNA repair. Biochemical and biophysical research communications 40 20493174
2018 Suppressing Ku70/Ku80 expression elevates homology-directed repair efficiency in primary fibroblasts. The international journal of biochemistry & cell biology 39 29655920
2013 Ku70 and ku80 null mutants improve the gene targeting frequency in Monascus ruber M7. Applied microbiology and biotechnology 39 23546425
2013 Ku70 and non-homologous end joining protect testicular cells from DNA damage. Journal of cell science 39 23857907
2024 LINC-PINT plays an anti-tumor role in nasopharyngeal carcinoma by binding to XRCC6 and affecting its function. Pathology, research and practice 38 39032384
2017 Ku70 Senses HTLV-1 DNA and Modulates HTLV-1 Replication. Journal of immunology (Baltimore, Md. : 1950) 36 28821586
2010 The DNA damage repair protein Ku70 interacts with FOXO4 to coordinate a conserved cellular stress response. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35 20570964
2019 NHEJ pathway is involved in post-integrational DNA repair due to Ku70 binding to HIV-1 integrase. Retrovirology 34 31690330
2010 Low expression of Ku70/80, but high expression of DNA-PKcs, predict good response to radiotherapy in early breast cancer. International journal of oncology 34 21042724
2022 circPRKAA1 activates a Ku80/Ku70/SREBP-1 axis driving de novo fatty acid synthesis in cancer cells. Cell reports 33 36417875
2011 Genetic polymorphisms in DNA double-strand break repair genes XRCC5, XRCC6 and susceptibility to hepatocellular carcinoma. Carcinogenesis 33 21304054
2007 Ku70/80 modulates ATM and ATR signaling pathways in response to DNA double strand breaks. The Journal of biological chemistry 33 17272272
2016 The Ku70/80 ring in Non-Homologous End-Joining: easy to slip on, hard to remove. Frontiers in bioscience (Landmark edition) 31 26709791
2013 Inhibition of Ku70 acetylation by INHAT subunit SET/TAF-Iβ regulates Ku70-mediated DNA damage response. Cellular and molecular life sciences : CMLS 31 24305947
2011 Ku70 alleviates neurodegeneration in Drosophila models of Huntington's disease. PloS one 31 22096569
2021 The Role of Ku70 as a Cytosolic DNA Sensor in Innate Immunity and Beyond. Frontiers in cellular and infection microbiology 30 34746031
2017 Deacetylation of Ku70 by SIRT6 attenuates Bax-mediated apoptosis in hepatocellular carcinoma. Biochemical and biophysical research communications 29 28238784
2023 SMYD2 inhibition-mediated hypomethylation of Ku70 contributes to impaired nonhomologous end joining repair and antitumor immunity. Science advances 28 37315132
2021 Cytoplasmic-translocated Ku70 senses intracellular DNA and mediates interferon-lambda1 induction. Immunology 28 33548066
2016 SIRT1 and LSD1 competitively regulate KU70 functions in DNA repair and mutation acquisition in cancer cells. Oncotarget 28 27384990
2013 The contribution of XRCC6/Ku70 to hepatocellular carcinoma in Taiwan. Anticancer research 28 23393345
2021 Ku70 suppresses alternative end joining in G1-arrested progenitor B cells. Proceedings of the National Academy of Sciences of the United States of America 27 34006647
2021 HMGB1 signaling phosphorylates Ku70 and impairs DNA damage repair in Alzheimer's disease pathology. Communications biology 27 34635772
2018 Sirt3 enhances glioma cell viability by stabilizing Ku70-BAX interaction. OncoTargets and therapy 27 30464504
2011 Accumulation of Ku70 at DNA double-strand breaks in living epithelial cells. Experimental cell research 27 21820429
2011 Genetic polymorphisms of DNA double strand break gene Ku70 and gastric cancer in Taiwan. BMC cancer 26 21575261
2016 EAF2 regulates DNA repair through Ku70/Ku80 in the prostate. Oncogene 24 27721405
2012 Interaction of caveolin-1 with Ku70 inhibits Bax-mediated apoptosis. PloS one 24 22745744
2009 DeubiKuitylation: a novel DUB enzymatic activity for the DNA repair protein, Ku70. Cell cycle (Georgetown, Tex.) 24 19448404
2024 m6A-mediated lnc-OXAR promotes oxaliplatin resistance by enhancing Ku70 stability in non-alcoholic steatohepatitis-related hepatocellular carcinoma. Journal of experimental & clinical cancer research : CR 23 39054531
2014 SMAR1 coordinates HDAC6-induced deacetylation of Ku70 and dictates cell fate upon irradiation. Cell death & disease 23 25299772
2011 Role of the XRCC5/XRCC6 dimer in carcinogenesis and pharmacogenomics. Pharmacogenomics 23 21521024
2016 Cytosolic Ku70 regulates Bax-mediated cell death. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 22 27488115
2015 Association between the XRCC6 polymorphisms and cancer risks: a systematic review and meta-analysis. Medicine 22 25569644
2022 The small nonstructural protein NP1 of human bocavirus 1 directly interacts with Ku70 and RPA70 and facilitates viral DNA replication. PLoS pathogens 21 35653410
2017 XRCC5 VNTR, XRCC6 -61C>G, and XRCC7 6721G>T Gene Polymorphisms Associated with Male Infertility Risk: Evidences from Case-Control and In Silico Studies. International journal of endocrinology 21 28421111
2016 The interaction of adenovirus E1A with the mammalian protein Ku70/XRCC6. Virology 21 27769014
2012 CREB-binding protein regulates Ku70 acetylation in response to ionization radiation in neuroblastoma. Molecular cancer research : MCR 21 23223795
2010 The DNA repair complex Ku70/86 modulates Apaf1 expression upon DNA damage. Cell death and differentiation 21 20966962
2008 Increased gene targeting in Ku70 and Xrcc4 transiently deficient human somatic cells. Molecular biotechnology 21 18759011
2000 Ku70 can translocate to the nucleus independent of Ku80 translocation and DNA-PK autophosphorylation. Biochemical and biophysical research communications 21 11027597
2008 Activities of DNA-PK and Ku86, but not Ku70, may predict sensitivity to cisplatin in human gliomas. Journal of neuro-oncology 20 18415044
2005 DNA end binding activity and Ku70/80 heterodimer expression in human colorectal tumor. World journal of gastroenterology 20 16425368
2002 Heterogeneous expression of Ku70 in human tissues is associated with morphological and functional alterations of the nucleus. The Journal of pathology 20 12210072
2016 Human Ku70 protein binds hairpin RNA and double stranded DNA through two different sites. Biochimie 19 27825805
2004 Expression of DNA-PKcs and Ku86, but not Ku70, differs between lymphoid malignancies. Experimental and molecular pathology 19 15215044
2002 Differential expression of DNA nonhomologous end-joining proteins Ku70 and Ku80 in melanoma progression. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 19 11950917
2024 Ku70 Binding to YAP Alters PARP1 Ubiquitination to Regulate Genome Stability and Tumorigenesis. Cancer research 18 38862269
2014 Association between polymorphisms at promoters of XRCC5 and XRCC6 genes and risk of breast cancer. Medical oncology (Northwood, London, England) 18 24615008
2012 The role of XRCC6 T-991C functional polymorphism in renal cell carcinoma. Anticancer research 18 22993329
2022 Integrated analysis reveals FOXA1 and Ku70/Ku80 as targets of ivermectin in prostate cancer. Cell death & disease 17 36050295
2020 C-Terminal Extensions of Ku70 and Ku80 Differentially Influence DNA End Binding Properties. International journal of molecular sciences 17 32937838
2012 Association of DNA double-strand break gene XRCC6 genotypes and lung cancer in Taiwan. Anticancer research 17 22399625
2011 Maspin increases Ku70 acetylation and Bax-mediated cell death in cancer cells. International journal of molecular medicine 17 22076034
2014 Regulation of ku70-bax complex in cells. Journal of cell death 16 25278782
2004 Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule. The Journal of biological chemistry 16 15123719

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