Affinage

USP36

Ubiquitin carboxyl-terminal hydrolase 36 · UniProt Q9P275

Length
1123 aa
Mass
122.9 kDa
Annotated
2026-06-11
51 papers in source corpus 35 papers cited in narrative 37 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

USP36 is a nucleolar deubiquitinating enzyme that coordinates ribosome biogenesis, rRNA processing, and the stability of numerous nucleolar and signaling proteins (PMID:19208757, PMID:29273634). It is recruited to the nucleolus through a C-terminal basic motif that is bound by nucleophosmin/B23, and there it removes polyubiquitin from substrates such as fibrillarin and nucleophosmin to protect them from proteasomal degradation, sustaining rRNA transcription/processing and cell proliferation (PMID:19208757, PMID:19679658). A defining activity is stabilization of the oncoprotein c-Myc: USP36 deubiquitinates c-Myc in the nucleolus, antagonizing SCF(Fbw7)-mediated turnover, and is itself a c-Myc target gene, forming a positive feedback loop (PMID:25775507). Beyond deubiquitination, USP36 is a non-canonical SUMO E3 ligase that interacts with SUMO2/Ubc9 and SUMOylates snoRNP and processing factors (Nop58, Nhp2, Nop56, DKC1, EXOSC10 at K583, Las1L at K565, DGCR8) to promote their RNA binding and rRNA/miRNA processing, often without altering substrate abundance (PMID:33852194, PMID:36912080, PMID:36950067, PMID:39356143). USP36 also processes ribosomal precursors directly: it cleaves the FUBI-eS30 fusion to drive late cytoplasmic 40S maturation, a dual ubiquitin/FUBI hydrolase activity explained by crystal structures of USP36 bound to ubiquitin and FUBI (PMID:34318747, PMID:37443395). Through deubiquitination it stabilizes a broad set of substrates linking it to DNA replication stress and repair (PrimPol via K29 chains, ALKBH5), histone H2B/H2A regulation, and multiple oncogenic and stress pathways (YAP, ERα, AR, survivin/cIAP1, RBM28-p53), with catalytic activity confirmed by the C131A inactive mutant in several contexts (PMID:33237263, PMID:36239338, PMID:29274341, PMID:36470870, PMID:39215346, PMID:38876304, PMID:39343961, PMID:38307305). Usp36 knockout is embryonic-lethal in mice, and in Drosophila its deubiquitinating activity is dispensable for viability and cell growth but essential for spermatogenesis, establishing both catalytic-dependent and catalytic-independent functions (PMID:29273634, PMID:40646716).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 2005 Medium

    Established USP36 as a bona fide deubiquitinase, providing the biochemical foundation for all subsequent substrate work.

    Evidence RT-PCR cloning, ubiquitin-cleavage activity assay, and domain analysis identifying a PEST motif and self-ubiquitination

    PMID:15809067

    Open questions at the time
    • No substrates identified
    • No cellular localization or pathway context
    • Single in vitro study
  2. 2009 High

    Defined USP36 as a nucleolar enzyme whose recruitment depends on nucleophosmin/B23 binding a C-terminal basic motif, answering where USP36 acts and how it is targeted.

    Evidence NLS mapping, reciprocal Co-IP, RNAi, in vitro deubiquitylation, and rRNA/proliferation readouts in human cells

    PMID:19208757 PMID:19679658

    Open questions at the time
    • Substrate repertoire beyond fibrillarin/nucleophosmin unknown at this stage
    • No structural basis for catalysis
  3. 2011 Medium

    Extended USP36 substrate range beyond the nucleolus by showing it stabilizes mitochondrial SOD2, hinting at functions outside ribosome biogenesis.

    Evidence 2D-gel/MALDI-TOF MS, yeast two-hybrid, Co-IP, and ubiquitination/half-life assays

    PMID:21268071

    Open questions at the time
    • Mechanism reconciling nucleolar localization with mitochondrial substrate unclear
    • Single lab
  4. 2012 High

    Connected USP36 to selective autophagy, showing its loss triggers p62/SQSTM1-dependent clearance of ubiquitinated cargo independent of TOR signaling.

    Evidence Drosophila loss-of-function genetics with RNAi confirmation in human cells and epistasis with p62

    PMID:22622177

    Open questions at the time
    • Direct substrates driving aggregate accumulation not defined
    • Link between nucleolar role and autophagy unclear
  5. 2015 High

    Identified c-Myc as a key USP36 substrate and a c-Myc/USP36 positive feedback loop, establishing USP36 as an oncogenic stabilizer that counteracts SCF(Fbw7).

    Evidence In vitro deubiquitination, cellular Co-IP, stability assays, and Fbw7 isoform-specific interaction mapping

    PMID:25775507

    Open questions at the time
    • Whether deubiquitination occurs only in the nucleolar compartment unresolved
    • In vivo tumor relevance not tested here
  6. 2017 High

    Demonstrated USP36 is essential in vivo and stabilizes the rRNA helicase DHX33, linking USP36 to early embryonic viability and translation.

    Evidence Usp36 knockout mouse (morula-to-blastocyst lethality), EM, Northern blot, OPP protein synthesis, ubiquitination assays

    PMID:29273634 PMID:29274341

    Open questions at the time
    • Catalytic dependence of essentiality not dissected
    • H2B deubiquitination role separate from rRNA functions not integrated
  7. 2020 High

    Revealed a DNA replication stress function by showing USP36 removes K29-linked chains from PrimPol and is itself stabilized after stress.

    Evidence Co-IP, MS, in vivo/in vitro ubiquitination assays with linkage specificity, and drug sensitivity assays

    PMID:33237263

    Open questions at the time
    • Upstream signal regulating USP36 stabilization not defined
    • Nucleolar vs nucleoplasmic site of PrimPol action unclear
  8. 2021 High

    Uncovered a second enzymatic mode—non-canonical SUMO E3 ligase activity—and direct FUBI-eS30 processing, redefining USP36 as a dual-activity ribosome biogenesis factor.

    Evidence In vitro SUMOylation reconstitution on snoRNP factors, snoRNA-binding and rRNA processing assays, plus in vitro FUBI cleavage with purified enzyme and CRISPRi/RNAi

    PMID:33852194 PMID:34318747

    Open questions at the time
    • How DUB, SUMO ligase, and protease activities are coordinated unknown
    • Structural basis for FUBI/ubiquitin discrimination not yet shown
  9. 2023 High

    Consolidated USP36 as a master nucleolar SUMO ligase across rRNA and miRNA processing machinery and provided structural explanation for its dual ubiquitin/FUBI specificity.

    Evidence Site-specific SUMOylation of EXOSC10 (K583) and DGCR8 with RNA-binding rescue, ALKBH5 deubiquitination in glioma models, and crystal structures of USP36 with ubiquitin and FUBI

    PMID:36239338 PMID:36912080 PMID:36950067 PMID:37443395

    Open questions at the time
    • Determinants selecting between SUMOylation versus deubiquitination of a given substrate unclear
    • Physiological hierarchy of substrates undefined
  10. 2024 High

    Expanded the catalytic-dependent substrate network into cancer and disease signaling, with C131A mutants confirming catalytic requirement and Las1L K565 SUMOylation linking USP36 to ITS2 processing.

    Evidence siRNA DUB screens, Co-IP, linkage-specific ubiquitination assays, C131A catalytic mutants, in vivo xenograft and cardiomyopathy mouse models, and site-specific SUMOylation rescue

    PMID:38307305 PMID:38876304 PMID:39215346 PMID:39343961 PMID:39356143

    Open questions at the time
    • Tissue-specific selection among many candidate substrates unresolved
    • Whether disease phenotypes depend on DUB vs SUMO activity often untested
  11. 2025 High

    Genetically separated catalytic from non-catalytic functions and identified post-translational regulation of USP36 itself.

    Evidence Drosophila CRISPR knock-in of catalytically inactive dUSP36 (viable but sterile) and metabolite-driven methylmalonylation of USP36 at K499 inhibiting its activity

    PMID:40646716 PMID:41398045

    Open questions at the time
    • Identity of catalytic-independent functions at the molecular level unknown
    • Generality of metabolic regulation across cell types untested

Open questions

Synthesis pass · forward-looking unresolved questions
  • How USP36 integrates its three activities (deubiquitination, SUMO ligation, FUBI/ubiquitin protease) and selects among its large substrate set in a given physiological or disease context remains unresolved.
  • No unifying model for substrate/activity selection
  • Mammalian catalytic-independent functions uncharacterized
  • Mechanism coupling nucleolar localization to non-nucleolar substrates unclear

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 7 GO:0016740 transferase activity 4 GO:0016787 hydrolase activity 3 GO:0016874 ligase activity 3
Localization
GO:0005730 nucleolus 4 GO:0005634 nucleus 2
Pathway
R-HSA-8953854 Metabolism of RNA 5 R-HSA-392499 Metabolism of proteins 4 R-HSA-1852241 Organelle biogenesis and maintenance 3 R-HSA-9612973 Autophagy 2 R-HSA-73894 DNA Repair 1
Partners
DGCR8DHX33EXOSC10FBLLAS1LMYCNPM1PRIMPOL
Complex memberships
Drosha-DGCR8 microprocessor (associated)

Evidence

Reading pass · 37 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2009 USP36 localizes to nucleoli via a C-terminal basic amino acid region and deubiquitinates nucleolar proteins nucleophosmin/B23 and fibrillarin, stabilizing them against ubiquitylation-mediated proteasomal degradation. RNAi-mediated depletion reduces rRNA transcription and processing, nucleolar morphology, and cell proliferation. Dominant-negative inhibition, RNAi knockdown, co-immunoprecipitation, in vitro deubiquitylation assay, subcellular fractionation/imaging Journal of cell science High 19208757
2009 Nucleophosmin/B23 recruits USP36 to nucleoli by interacting with a specific basic amino acid motif (RGKEKKIKKFKREKRR) in USP36's C-terminal region. Knockdown of nucleophosmin/B23 reduces nucleolar USP36 levels and elevates fibrillarin ubiquitylation. Nucleolar localization signal mapping, Co-IP, RNAi knockdown, ubiquitylation assay The Journal of biological chemistry High 19679658
2005 USP36 possesses deubiquitinase activity (cleaves ubiquitin from substrates), contains a PEST motif, and is itself polyubiquitinated. RT-PCR cloning, ubiquitin-cleavage activity assay, immunoprecipitation, structural domain analysis Biochemical and biophysical research communications Medium 15809067
2011 USP36 interacts with mitochondrial antioxidant enzyme SOD2, reduces its polyubiquitination, and stabilizes it against proteasomal degradation, extending its half-life. 2D-gel electrophoresis, MALDI-TOF/MS identification, co-immunoprecipitation, yeast two-hybrid, ubiquitination assay Journal of cellular biochemistry Medium 21268071
2012 USP36 (dUsp36) loss of function in Drosophila and human cells activates p62/SQSTM1-dependent selective autophagy of ubiquitinated cargo; dUSP36 loss leads to nuclear accumulation of ubiquitinated proteins (including histone H2B) and cytoplasmic ubiquitinated aggregates eliminated by autophagy. dUSP36 is not part of the TOR signaling pathway. Drosophila loss-of-function genetics, RNAi in human cells, p62/SQSTM1 requirement epistasis, fluorescence imaging of ubiquitin aggregates Autophagy High 22622177
2015 USP36 is a nucleolar deubiquitinase for c-Myc: it interacts with and deubiquitinates c-Myc in cells and in vitro, stabilizing it against SCF(Fbw7)-mediated degradation in the nucleolus. USP36 interacts with nucleolar Fbw7γ but not nucleoplasmic Fbw7α, yet abolishes c-Myc degradation by both. USP36 is also a c-Myc target gene, forming a positive feedback loop. Co-immunoprecipitation, in vitro deubiquitination assay, knockdown, c-Myc stability assay, isoform-specific interaction mapping Proceedings of the National Academy of Sciences of the United States of America High 25775507
2016 USP36 binds Nedd4-2 and regulates its association with TrkA neurotrophin receptor indirectly (USP36 does not directly deubiquitinate TrkA). Depletion of USP36 increases TrkA–Nedd4-2 complex formation, enhancing TrkA ubiquitination; USP36 overexpression disrupts this complex. USP36 also interferes with Nedd4-2-dependent regulation of Kv7.2/3 channels. siRNA screen, Co-IP, overexpression/depletion, TrkA ubiquitination assay, PC12 differentiation assay The Journal of biological chemistry Medium 27445338
2017 USP36 deubiquitinates and stabilizes the DEAH-box RNA helicase DHX33 in the nucleolus. Usp36 knockout in mice is lethal at the morula-to-blastocyst transition. USP36 depletion impairs rRNA synthesis, protein translation, cell proliferation, and induces apoptosis and cell cycle arrest in human cancer cells. Usp36 knockout mouse model, electron microscopy, Northern blot, O-propargyl-puromycin protein synthesis assay, ubiquitination assay, Co-IP The Journal of biological chemistry High 29273634
2017 USP36 is a histone H2B deubiquitinase: it interacts with H2B and deubiquitinates monoubiquitinated H2B (H2Bub1) in cells and in vitro. USP36 depletion increases H2Bub1 at the p21 locus gene body, inducing p21 expression and inhibiting cell proliferation. Co-IP, in vitro deubiquitination assay, overexpression, knockdown, ChIP at p21 locus Biochemical and biophysical research communications Medium 29274341
2018 USP36 controls cellular localization of CHD7; in neuroblastoma, lncRNAs CASC15/NBAT1 modulate USP36 localization, which in turn regulates CHD7 stability and downstream SOX9 expression. Loss-of-function experiments, co-immunoprecipitation, immunoblotting, localization studies Cancer cell Medium 29533783
2018 USP36 deubiquitinates and stabilizes PME-1 (protein phosphatase methylesterase 1), thereby promoting ERK and Akt signaling pathways. Co-IP, USP36 knockdown/overexpression, ubiquitination assay, western blot for ERK/Akt activation FEBS letters Medium 29577269
2019 USP36 knockdown impairs Parkin-dependent mitophagy by reducing mRNA and protein levels of Beclin-1 and ATG14L (without altering nucleolar USP36 localization during mitophagy); restoration of ATG14L rescues mitophagy in USP36-silenced cells. High-content imaging siRNA screen, knockdown, immunofluorescence, western blot, ATG14L overexpression rescue Experimental cell research Medium 31550441
2020 USP36 interacts with PrimPol, deubiquitinates K29-linked polyubiquitin chains on PrimPol, and increases PrimPol protein stability in response to DNA replication stress. USP36 is itself deubiquitinated following replication stress, facilitating its upregulation. Co-IP, mass spectrometry, in vivo and in vitro ubiquitination assays, replication stress assays, cisplatin/olaparib sensitivity Nucleic acids research High 33237263
2021 USP36 promotes nucleolar SUMOylation: it interacts with SUMO2 and Ubc9, directly mediates SUMOylation in cells and in vitro, and specifically SUMOylates snoRNP components Nop58, Nhp2, Nop56, and DKC1, promoting their binding to snoRNAs. USP36 knockdown markedly impairs rRNA processing and translation. Overexpression, knockdown, genetic deletion, Co-IP, in vitro SUMOylation assay, snoRNA binding assay, rRNA processing assay EMBO reports High 33852194
2021 USP36 cleaves the ribosomal fusion protein FUBI-eS30: purified USP36 cuts FUBI-eS30 in vitro, and USP36 depletion by RNAi or CRISPRi impairs FUBI-eS30 processing and late steps of cytoplasmic 40S maturation including 18S rRNA maturation and recycling of biogenesis factors. Differential affinity purification, RNAi, CRISPRi, in vitro processing assay with purified USP36, rRNA processing analysis eLife High 34318747
2021 USP36 deubiquitinates and stabilizes DOCK4 (a guanine nucleotide exchange factor); increased USP36-DOCK4 stabilization activates Wnt/β-catenin signaling and promotes epithelial-to-mesenchymal transition in diabetic renal tubular epithelial cells. Co-IP, deubiquitination assay, knockdown/overexpression, Wnt pathway reporter Frontiers in cell and developmental biology Medium 33968925
2022 USP36 deubiquitinates and stabilizes YAP by blocking K48-linked polyubiquitination, thereby promoting Hippo/YAP signaling in esophageal squamous carcinoma. siRNA DUB screening, Co-IP, ubiquitination assay, knockdown/overexpression, western blot Cell death & disease Medium 36470870
2022 USP36 activates Snail2 deubiquitination in glioblastoma, downstream of PRL1 oncogene; PRL1 promotes EMT and invasion by activating USP36-mediated Snail2 stabilization. Co-IP, ubiquitination assay, knockdown/overexpression, in vitro and in vivo tumorigenesis assays Frontiers in oncology Medium 35111679
2023 USP36 interacts with EXOSC10 (nucleolar RNA exosome subunit) and mediates its SUMOylation at K583. K583 SUMOylation is required for EXOSC10 binding to pre-rRNAs; K583R mutant fails to rescue rRNA processing and cell growth defects upon EXOSC10 knockdown. USP36 does not significantly regulate EXOSC10 protein levels. Co-IP, in vitro SUMOylation assay, mutant rescue, rRNA processing assay, RNA binding assay Nucleic acids research High 36912080
2023 USP36 deubiquitinates and stabilizes ALKBH5, regulating ALKBH5-mediated gene expression in glioblastoma. Depletion of USP36 impairs glioma stem cell self-renewal, proliferation, and sensitizes cells to temozolomide. DUB siRNA library screen, mass spectrometry, Co-IP, in vivo and in vitro ubiquitination assays, neurosphere formation, intracranial tumor growth Neuro-oncology High 36239338
2023 Crystal structures of USP36 in complex with ubiquitin and Fubi reveal the substrate recognition mechanism for dual ubiquitin/Fubi cleavage activity. USP16 was also identified as having dual ubiquitin/Fubi cleavage activity by chemoproteomics; both USP16 and USP36 participate in Fubi-S30 maturation. Crystal structure determination, chemoproteomics, Fubi C-terminal hydrolase activity assay, substrate specificity analysis Nature chemical biology High 37443395
2023 Ribotoxic stress activates JNK, which in turn activates USP36, leading to stabilization of Snail1 in the nucleolus. Nucleolar Snail1 facilitates ribosome biogenesis and tumor cell survival, and mediates solid tumor resistance to homoharringtonine (HHT). Co-IP, knockdown, JNK inhibition, HHT treatment, ribosome biogenesis assay, combination therapy in vivo Nature communications Medium 37833415
2023 USP36 associates with the Drosha-DGCR8 microprocessor complex and mediates DGCR8 SUMOylation by SUMO2. This SUMOylation promotes DGCR8 binding to pri-miRNAs without affecting DGCR8 levels or the Drosha-DGCR8 complex. USP36 knockdown attenuates pri-miRNA processing and reduces mature miRNA levels. Co-IP, in vitro/in vivo SUMOylation assay, SUMO site mapping, RNA immunoprecipitation, knockdown, cell proliferation assay Cancer research communications High 36950067
2024 USP36 binds PARP1 and mediates its deubiquitination, increasing PARP1 protein stability in cardiomyocytes exposed to doxorubicin. The catalytically inactive C131A mutant of USP36 fails to stabilize PARP1, confirming catalytic dependence. Cardiac knockdown of USP36 (rAAV9-shUSP36) preserved cardiac function in a doxorubicin-induced cardiomyopathy mouse model. Co-IP, ubiquitination assay, catalytic mutant (C131A), rAAV9 in vivo knockdown, cardiac function assessment Cellular signalling High 38307305
2024 USP36 deubiquitinates survivin (removing K48-linked chains) and cIAP1 (removing K11-linked chains), stabilizing both anti-apoptotic proteins. USP36 disrupts XIAP-SMAC complex formation and promotes RIP1 ubiquitination, thereby inhibiting both intrinsic and extrinsic apoptosis in colorectal cancer cells. siRNA gene silencing, Co-IP, ubiquitin linkage-specific assays, apoptosis assays The Journal of biological chemistry Medium 38876304
2024 USP36 deubiquitinates ERα, inhibiting K48-linked polyubiquitination and stabilizing ERα protein; the catalytically inactive C131A mutant fails to promote breast cancer progression through ERα signaling. USP36 knockdown destabilizes the tamoxifen-resistant ERα mutant (Y537S). DUB siRNA library screen, Co-IP, ubiquitination assay, catalytic mutant (C131A), xenograft model, RNA sequencing Journal of experimental & clinical cancer research : CR High 39215346
2024 USP36 deubiquitinates and stabilizes RBM28 at K162; upregulated RBM28 binds p53 and suppresses its transcriptional activity, inactivating the p53 signaling pathway in colorectal cancer. Co-IP, ubiquitination assay (site-specific K162), knockdown/overexpression, p53 transcription reporter Oncogene Medium 39343961
2024 USP36 SUMOylates Las1L at K565; K565 SUMOylation is essential for ITS2 pre-rRNA processing but not for Las1L protein levels or Las1L-Nol9 complex formation. USP36 also deubiquitinates Las1L and Nol9, regulating their stability. Co-IP, in vitro SUMOylation assay, K565R mutant rescue, ITS2 processing assay, ubiquitination assay Cancer research communications High 39356143
2024 USP36 interacts with WDR5 and stabilizes it via deubiquitination; USP36 knockdown increases WDR5 ubiquitination and degradation, impairing osteogenic differentiation. Overexpression of WDR5 rescues the differentiation defects in USP36-deficient osteoblasts. Co-IP, ubiquitination assay, knockdown, Alizarin red staining, WDR5 rescue overexpression Journal of orthopaedic surgery and research Medium 39152465
2024 A germline USP36 variant (rs3744797, K814N) stabilizes MLLT3 via deubiquitination in the nucleolus, activating HIF1α and Snai downstream signaling and conferring resistance to EGFR-TKIs in non-small cell lung cancer. m6A-genome-wide-variant analysis, Co-IP, ubiquitination assay, in vitro/in vivo functional assays, patient cohort Clinical cancer research : an official journal of the American Association for Cancer Research Medium 38261467
2025 MMA (methylmalonic acid) induces methylmalonylation of USP36 at K499, inhibiting USP36-mediated deubiquitination and SUMOylation of SUFU, thereby promoting GLI1 expression and Hedgehog pathway activation in clear-cell renal cell carcinoma macrophages. Metabolomic profiling, site-specific modification analysis, ubiquitination and SUMOylation assays, in vitro and in vivo functional experiments Cell death and differentiation Medium 41398045
2025 In Drosophila, catalytically inactive dUSP36 (CRISPR knock-in) allows survival to adulthood with minor growth defects, demonstrating that the deubiquitinating activity is dispensable for cell growth but essential for spermatogenesis, revealing both catalytic-dependent and catalytic-independent mechanisms. CRISPR/Cas9 catalytic mutation knock-in, null mutant comparison, viability and fertility assays Genetics High 40646716
2025 USP36 stabilizes APEX1 by removing K48-linked ubiquitin chains, suppressing ferroptosis in melanoma cells. USP36 knockdown sensitizes xenograft tumors to ferroptosis, and APEX1 knockdown abolishes USP36's anti-ferroptotic effect. Co-IP, ubiquitination assay (K48-specific), overexpression/knockdown, erastin treatment, xenograft model Clinical and experimental medicine Medium 41649582
2025 USP36 functions as a SUMO ligase for GNL3 (nucleolar GTP-binding protein), while SENP3 deSUMOylates GNL3. GNL3 SUMOylation is required for interaction with the BLM-DNA2 complex and DNA end resection in homologous recombination repair. Co-IP, in vitro SUMOylation assay, CRISPR/RNAi knockdown, DNA end resection assay, epistasis with SENP3 bioRxivpreprint Medium
2025 USP36 stabilizes KIF2C by removing K48-linked ubiquitin chains, suppressing ferroptosis in breast cancer cells. USP36-deficient xenograft tumors show reduced proliferation and increased ferroptosis. Co-IP, K48-specific ubiquitination assay, knockdown/overexpression, erastin ferroptosis assay, xenograft model Biochemical pharmacology Medium 40744233
2025 USP36 deubiquitinates and stabilizes AR (androgen receptor) in prostate cancer cells under oxidative stress (H2O2); USP36–AR interaction is induced by H2O2 and knockdown of USP36 abolishes H2O2-induced activation of the AR-PSA pathway. TurboID proximity labeling plus mass spectrometry, Co-IP, ubiquitination assay, dual-luciferase reporter, knockdown Scientific reports Medium 41298501
2025 FBL (fibrillarin) acts as a carrier regulating the balance between BMI1-mediated H2A monoubiquitination and USP36-mediated H2Aub deubiquitination in nucleolar lysosome-like structures during glucose starvation; USP36, FBL, Midnolin, and BMI1 form a complex responsible for H2Aub degradation. Protein interaction screening, Co-IP, knockdown, cell cycle and viability assays bioRxivpreprint Low

Source papers

Stage 0 corpus · 51 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2015 The nucleolar ubiquitin-specific protease USP36 deubiquitinates and stabilizes c-Myc. Proceedings of the National Academy of Sciences of the United States of America 186 25775507
2018 Sense-Antisense lncRNA Pair Encoded by Locus 6p22.3 Determines Neuroblastoma Susceptibility via the USP36-CHD7-SOX9 Regulatory Axis. Cancer cell 112 29533783
2009 Nucleolar structure and function are regulated by the deubiquitylating enzyme USP36. Journal of cell science 73 19208757
2012 The deubiquitinating enzyme USP36 controls selective autophagy activation by ubiquitinated proteins. Autophagy 61 22622177
2023 USP36 promotes tumorigenesis and drug sensitivity of glioblastoma by deubiquitinating and stabilizing ALKBH5. Neuro-oncology 44 36239338
2009 Nucleophosmin/B23 regulates ubiquitin dynamics in nucleoli by recruiting deubiquitylating enzyme USP36. The Journal of biological chemistry 44 19679658
2020 The deubiquitinase USP36 Regulates DNA replication stress and confers therapeutic resistance through PrimPol stabilization. Nucleic acids research 40 33237263
2017 Loss of the deubiquitinase USP36 destabilizes the RNA helicase DHX33 and causes preimplantation lethality in mice. The Journal of biological chemistry 38 29273634
2015 Deubiquitinating c-Myc: USP36 steps up in the nucleolus. Cell cycle (Georgetown, Tex.) 35 26697836
2011 Protein stability of mitochondrial superoxide dismutase SOD2 is regulated by USP36. Journal of cellular biochemistry 34 21268071
2022 USP36 facilitates esophageal squamous carcinoma progression via stabilizing YAP. Cell death & disease 32 36470870
2020 Oxidized low-density lipoprotein accelerates the injury of endothelial cells via circ-USP36/miR-98-5p/VCAM1 axis. IUBMB life 32 33249762
2021 The deubiquitinase USP36 promotes snoRNP group SUMOylation and is essential for ribosome biogenesis. EMBO reports 31 33852194
2019 Ubiquitin-specific protease USP36 knockdown impairs Parkin-dependent mitophagy via downregulation of Beclin-1-associated autophagy-related ATG14L. Experimental cell research 30 31550441
2005 Deubiquitinating enzyme USP36 contains the PEST motif and is polyubiquitinated. Biochemical and biophysical research communications 28 15809067
2023 USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress. Nature communications 27 37833415
2021 Processing of the ribosomal ubiquitin-like fusion protein FUBI-eS30/FAU is required for 40S maturation and depends on USP36. eLife 25 34318747
2017 The ubiquitin-specific protease USP36 is a conserved histone H2B deubiquitinase. Biochemical and biophysical research communications 24 29274341
2008 Differential display identifies overexpression of the USP36 gene, encoding a deubiquitinating enzyme, in ovarian cancer. International journal of medical sciences 23 18566677
2023 The ubiquitin-specific protease USP36 SUMOylates EXOSC10 and promotes the nucleolar RNA exosome function in rRNA processing. Nucleic acids research 21 36912080
2023 Molecular basis for ubiquitin/Fubi cross-reactivity in USP16 and USP36. Nature chemical biology 21 37443395
2021 USP36-Mediated Deubiquitination of DOCK4 Contributes to the Diabetic Renal Tubular Epithelial Cell Injury via Wnt/β-Catenin Signaling Pathway. Frontiers in cell and developmental biology 19 33968925
2020 Circ_USP36/miR-182-5p/KLF5 axis regulates the ox-LDL-induced injury in human umbilical vein smooth muscle cells. American journal of translational research 18 33437365
2022 USP36 promotes tumor growth of non-small cell lung cancer via increasing KHK-A expression by regulating c-MYC-hnRNPH1/H2 axis. Human cell 16 35133629
2019 USP36 protects proximal tubule cells from ischemic injury by stabilizing c-Myc and SOD2. Biochemical and biophysical research communications 16 30975468
2021 Circ_USP36 Silencing Attenuates Oxidized Low-Density Lipoprotein-Induced Dysfunction in Endothelial Cells in Atherosclerosis Through Mediating miR-197-3p/ROBO1 Axis. Journal of cardiovascular pharmacology 15 34369900
2024 USP36-mediated PARP1 deubiquitination in doxorubicin-induced cardiomyopathy. Cellular signalling 14 38307305
2024 USP36 inhibits apoptosis by deubiquitinating cIAP1 and survivin in colorectal cancer cells. The Journal of biological chemistry 14 38876304
2024 USP36 promotes tumorigenesis and tamoxifen resistance in breast cancer by deubiquitinating and stabilizing ERα. Journal of experimental & clinical cancer research : CR 13 39215346
2022 miR-140-3p/usp36 axis mediates ubiquitination to regulate PKM2 and suppressed the malignant biological behavior of breast cancer through Warburg effect. Cell cycle (Georgetown, Tex.) 13 36305548
2021 NBAT1/CASC15-003/USP36 control MYCN expression and its downstream pathway genes in neuroblastoma. Neuro-oncology advances 13 34056606
2016 Ubiquitin-specific Protease 36 (USP36) Controls Neuronal Precursor Cell-expressed Developmentally Down-regulated 4-2 (Nedd4-2) Actions over the Neurotrophin Receptor TrkA and Potassium Voltage-gated Channels 7.2/3 (Kv7.2/3). The Journal of biological chemistry 12 27445338
2024 The Emerging Role of Ubiquitin-Specific Protease 36 (USP36) in Cancer and Beyond. Biomolecules 11 38785979
2023 The Ubiquitin-specific Protease USP36 Associates with the Microprocessor Complex and Regulates miRNA Biogenesis by SUMOylating DGCR8. Cancer research communications 11 36950067
2024 Cinobufotalin regulates the USP36/c-Myc axis to suppress malignant phenotypes of colon cancer cells in vitro and in vivo. Aging 10 38517383
2024 USP36 promotes colorectal cancer progression through inhibition of p53 signaling pathway via stabilizing RBM28. Oncogene 10 39343961
2022 PRL1 Promotes Glioblastoma Invasion and Tumorigenesis via Activating USP36-Mediated Snail2 Deubiquitination. Frontiers in oncology 10 35111679
2025 Resveratrol targets mitochondrial USP36-SOD2 to induce autophagy-ferroptosis and inhibit gastric cancer progression. Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 9 40650854
2018 PME-1 is regulated by USP36 in ERK and Akt signaling pathways. FEBS letters 8 29577269
2024 Germline USP36 Mutation Confers Resistance to EGFR-TKIs by Upregulating MLLT3 Expression in Patients with Non-Small Cell Lung Cancer. Clinical cancer research : an official journal of the American Association for Cancer Research 6 38261467
2024 USP36 regulates the proliferation, survival, and differentiation of hFOB1.19 osteoblast. Journal of orthopaedic surgery and research 4 39152465
2024 USP36 SUMOylates Las1L and Promotes Its Function in Pre-Ribosomal RNA ITS2 Processing. Cancer research communications 4 39356143
2025 Targeting MMA-induced USP36 methylmalonylation to suppress macrophage polarization and tumor progression in clear-cell renal cell carcinoma. Cell death and differentiation 3 41398045
2024 SUMOylation regulation of ribosome biogenesis: Emerging roles for USP36. Frontiers in RNA research 3 38764604
2024 USP36 plays an oncogenic role in colorectal cancer cells. Neoplasma 1 38215036
2024 Bufalin: A promising therapeutic drug against the cisplatin-resistance of ovarian cancer by targeting the USP36/c-Myc axis. Biochemical and biophysical research communications 1 39067250
2026 USP36 inhibits ferroptosis of melanoma cells by stabilizing APEX1. Clinical and experimental medicine 0 41649582
2025 The deubiquitinase USP36 funtions through catalytic-dependent and catalytic-independent mechanisms in Drosophila. Genetics 0 40646716
2025 USP36 suppresses breast cancer cell ferroptosis by regulating the ubiquitination and stability of KIF2C. Biochemical pharmacology 0 40744233
2025 The role of USP36 in ribosome biogenesis and other pathophysiological processes. Frontiers in molecular biosciences 0 40909126
2025 Oxidative stress reactivates androgen receptor signaling via USP36 to drive castration resistance in prostate cancer. Scientific reports 0 41298501

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