Affinage

TRAIP

E3 ubiquitin-protein ligase TRAIP · UniProt Q9BWF2

Length
469 aa
Mass
53.3 kDa
Annotated
2026-06-10
38 papers in source corpus 28 papers cited in narrative 26 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

TRAIP is a RING-domain E3 ubiquitin ligase that operates at the DNA replication machinery to safeguard genome integrity during S-phase and mitosis (PMID:30842657, PMID:26711499). It localizes to active and stressed replication forks through a conserved PIP-box interaction with PCNA, where it promotes ATR-dependent checkpoint signaling and replication fork progression through DNA lesions; loss of TRAIP causes chromosomal instability, and biallelic mutations cause microcephalic primordial dwarfism (PMID:26711499, PMID:26595769). At the heart of its activity, TRAIP ubiquitylates the CMG replicative helicase (CDC45-MCM2-7-GINS): when two forks converge at an interstrand crosslink, short ubiquitin chains recruit NEIL3 glycosylase to unhook the crosslink directly while longer chains trigger p97/VCP-dependent CMG unloading to license the Fanconi anaemia pathway, making TRAIP the switch between the two ICL repair routes (PMID:30842657). In mitosis, TRAIP drives disassembly of all residual replisomes — including stalled ones — via K6/K63-linked ubiquitylation of MCM7 and p97-dependent extraction, an activity essential for mitotic DNA synthesis (MiDAS) and accurate chromosome segregation (PMID:31545170, PMID:30979826). This mitotic activity is gated by Cyclin B-CDK1 phosphorylation, which licenses TRAIP autoubiquitylation and CMG ubiquitylation that it cannot perform on terminated replisomes in S-phase (PMID:40637231); the opposing USP37 deubiquitylase removes untimely CMG ubiquitylation to prevent premature replisome disassembly (PMID:40533495, PMID:40411782). TRAIP additionally resolves replication-transcription conflicts during S-phase, an activity required for continued proliferation (PMID:37604812), and it recruits RAP80/BRCA1 to lesions via RNF20-RNF40 to promote homologous recombination (PMID:26781088). TRAIP is itself regulated by SUMOylation, which sustains its nuclear retention and stability (PMID:26820530), and homodimerizes through its coiled-coil domain to control mitotic progression (PMID:26093298). Beyond the replisome, TRAIP ubiquitylates context-specific substrates including MYC and KANK1 (PMID:38123820, PMID:34349117).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 2014 Medium

    Established the first cellular role for TRAIP's catalytic activity by showing it is required for spindle assembly checkpoint integrity, linking the ligase to faithful mitosis.

    Evidence RNAi knockdown in HeLa cells with live imaging, kinetochore MAD2 quantification, and WT-versus-catalytic-dead rescue

    PMID:25335891

    Open questions at the time
    • No direct ubiquitylation substrate identified at the kinetochore
    • Mechanism by which TRAIP maintains MAD2 levels unresolved
    • Single-lab observation
  2. 2015 High

    Tied TRAIP physically and functionally to the replisome by identifying a PCNA-binding PIP box and a requirement for both ligase activity and PCNA binding in ATR checkpoint signaling, defining TRAIP as a replication-stress factor.

    Evidence Reciprocal Co-IP, PIP-box mutagenesis, RPA-ssDNA and Chk1 phosphorylation readouts, clonogenic survival in human cells

    PMID:26711499

    Open questions at the time
    • Direct ubiquitylation substrate during replication stress not yet defined
    • How PIP-box binding couples to ATR activation mechanistically unclear
  3. 2015 High

    Connected TRAIP to human disease and to lesion bypass, showing it relocalizes to damage in S-phase, supports H2AX/RPA2 phosphorylation and fork progression, and that mutations cause microcephalic primordial dwarfism.

    Evidence Patient genetics, laser microirradiation foci, phosphorylation assays, DNA fiber assays, siRNA

    PMID:26595769

    Open questions at the time
    • Causal substrate linking TRAIP to the phosphorylation events not identified
    • Mechanism linking molecular defect to dwarfism phenotype unresolved
  4. 2016 High

    Placed TRAIP upstream in homologous recombination by mapping an N-terminal RAP80 interaction and a C-terminal RNF20-RNF40 interaction that recruits TRAIP to damage and feeds the RAP80/BRCA1 axis.

    Evidence Co-IP, domain deletion, laser microirradiation recruitment, HR reporter assays

    PMID:26781088 PMID:27462463

    Open questions at the time
    • Whether RAP80/BRCA1 recruitment depends on TRAIP catalytic activity not fully resolved
    • Relationship between this HR role and the fork-protection role unclear
  5. 2016 Medium

    Identified post-translational and structural controls on TRAIP itself — SUMOylation for nuclear retention/stability and nucleolar residence coupled to active rDNA transcription.

    Evidence SUMO-site mutagenesis with fractionation and cycloheximide chase; RNA Pol I inhibition, rDNA damage, and DNase/RNase release with live imaging

    PMID:26820530 PMID:30165463

    Open questions at the time
    • SUMO E3 ligase responsible for TRAIP modification unknown
    • Functional purpose of nucleolar sequestration not established
  6. 2019 High

    Defined TRAIP's central biochemical activity: ubiquitylation of the CMG helicase at converged forks, with chain length dictating the choice between NEIL3-mediated crosslink unhooking and p97-dependent CMG unloading into the Fanconi anaemia pathway.

    Evidence Xenopus egg extract reconstitution with chain-length analysis, NEIL3 binding, p97 inhibition, and Fanconi pathway epistasis

    PMID:30842657

    Open questions at the time
    • How chain length is enzymatically tuned in vivo unclear
    • Reader specificity distinguishing short versus long chains not fully defined
  7. 2019 High

    Generalized TRAIP to a dedicated mitotic replisome-disassembly factor, showing it removes residual/stalled replisomes via K6/K63 chains on MCM7 and p97, and is essential for MiDAS and accurate segregation.

    Evidence C. elegans and human cell loss-of-function, Xenopus extract with linkage-specific ubiquitin analysis and p97 inhibition, live imaging of segregation errors

    PMID:30979826 PMID:31545170

    Open questions at the time
    • Mechanism restricting this activity to mitosis not addressed in these studies
    • How K6/K63 chains are specified at MCM7 unknown
  8. 2021 High

    Positioned TRAIP within the broader replisome-disassembly framework, showing metazoans use a conserved pair of ligases (S-phase CUL2-LRR1 and mitotic TRAIP) for CMG unloading.

    Evidence Mouse ESC knockouts with chromatin fractionation, ubiquitylation assays, and cell-cycle staging

    PMID:33590678

    Open questions at the time
    • Division of labor between the two ligases under different stress contexts not fully mapped
  9. 2023 High

    Established an S-phase-specific function in resolving replication-transcription conflicts essential for proliferation, distinguishing it from TRAIP's mitotic role.

    Evidence Auxin-induced degron for cell-cycle-specific depletion, gamma-H2AX at transcription start sites, cell cycle and senescence assays

    PMID:37604812

    Open questions at the time
    • Direct substrate at conflict sites not identified
    • Whether CMG ubiquitylation underlies conflict resolution unclear
  10. 2024 High

    Solved how TRAIP is restricted to mitosis, showing CDK1 phosphorylation builds a TRAIP-TTF2-Pol epsilon bridge that tethers TRAIP to replisomes to drive CMG unloading and fork breakage.

    Evidence Xenopus extract with AlphaFold-Multimer-guided mutagenesis, TTF2 zinc-finger binding, CDK1 phosphorylation, CMG unloading and MiDAS assays (preprint)

    PMID:bio_10.1101_2024.11.30.626186 PMID:bio_10.1101_2024.12.01.626218

    Open questions at the time
    • Awaits peer review
    • How the bridge selectively recognizes stalled versus active replisomes not fully resolved
  11. 2025 High

    Refined the cell-cycle gating model: CDK phosphorylation activates TRAIP autoubiquitylation and CMG ubiquitylation, and is necessary but not sufficient — TRAIP binds terminated replisomes in S-phase without ubiquitylating them.

    Evidence Auxin degron, CDK inhibition, phospho-mimetic/non-phosphorylatable mutants, in vitro ubiquitylation with mitotic chromatin, MiDAS assays

    PMID:40637231

    Open questions at the time
    • The additional mitotic input beyond CDK phosphorylation not fully defined
    • Structural basis of activation not resolved
  12. 2025 High

    Identified the antagonist that constrains TRAIP timing: USP37 deubiquitylase binds CMG via CDC45 and reverses premature TRAIP-dependent CMG ubiquitylation, with TRAIP loss suppressing USP37-KO topoisomerase-inhibitor sensitivity.

    Evidence Xenopus extract depletion, USP37-knockout cells, genetic epistasis, CMG ubiquitylation assays, structure-guided CDC45-binding mutations

    PMID:40411782 PMID:40533495

    Open questions at the time
    • How USP37 activity is itself temporally regulated unclear
    • Whether USP37 acts on mitotic as well as S-phase TRAIP activity not fully delineated
  13. 2023 Medium

    Extended TRAIP function to context-specific substrate ubiquitylation, identifying MYC as a direct K48-ubiquitylation target in a negative feedback loop and KANK1 in osteosarcoma signaling.

    Evidence Co-IP, site-specific ubiquitylation mutagenesis, ChIP, proteasome inhibition and rescue in cancer cell lines

    PMID:34349117 PMID:38123820

    Open questions at the time
    • No in vitro reconstitution of direct ubiquitylation for KANK1
    • Relationship between these cancer-context substrates and the core replisome role unclear

Open questions

Synthesis pass · forward-looking unresolved questions
  • How TRAIP's distinct substrate selectivities (CMG, MYC, KANK1, histone marks) are coordinated and partitioned across S-phase, mitosis, and specific cellular contexts remains unresolved.
  • No unifying determinant of substrate choice identified
  • Structural basis of the catalytic activation switch not solved
  • Mechanism connecting molecular defects to the dwarfism phenotype unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016740 transferase activity 4 GO:0140096 catalytic activity, acting on a protein 3 GO:0016874 ligase activity 2
Localization
GO:0005694 chromosome 3 GO:0005730 nucleolus 2 GO:0005634 nucleus 1
Pathway
R-HSA-1640170 Cell Cycle 3 R-HSA-392499 Metabolism of proteins 3 R-HSA-69306 DNA Replication 3 R-HSA-73894 DNA Repair 3
Partners
MYCPCNARAP80RNF20RNF40TTF2USP37ZNF212
Complex memberships
CMG replicative helicase (substrate-bound)TRAIP-TTF2-DNA polymerase epsilon

Evidence

Reading pass · 26 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2019 TRAIP is an E3 ubiquitin ligase that ubiquitylates the CMG replicative helicase (CDC45-MCM2-7-GINS) when two replisomes converge at a DNA interstrand crosslink (ICL). Short ubiquitin chains on CMG recruit NEIL3 glycosylase (which can directly cleave the crosslink), whereas longer ubiquitin chains trigger CMG unloading by the p97 ATPase, enabling the Fanconi anaemia pathway. TRAIP thereby controls the choice between the two known pathways of replication-coupled ICL repair. Xenopus egg extract reconstitution; biochemical fractionation; ubiquitin chain-length analysis; NEIL3 binding assays; p97 inhibition; epistasis with Fanconi anaemia pathway mutants Nature High 30842657
2019 TRAIP ubiquitin ligase drives replisome disassembly in response to incomplete DNA replication in mitosis (mitotic replisome disassembly), providing access to replication forks for other factors; TRAIP is also essential for mitotic DNA repair synthesis (MiDAS) in human cells, and its loss causes mitotic segregation errors. C. elegans embryo genetics; human cell knockdown; epistasis analysis; live imaging of chromosome segregation errors eLife High 31545170
2019 In Xenopus egg extracts, mitotic replisome disassembly depends on TRAIP-mediated formation of K6- and K63-linked ubiquitin chains on MCM7, followed by p97/VCP-dependent extraction. This pathway does not require SUMO modifications (unlike lower eukaryotes) and can remove all replisomes from mitotic chromatin, including stalled ones. Xenopus egg extract; ubiquitin linkage-specific analysis; p97 inhibition; SUMO pathway manipulation Life science alliance High 30979826
2015 TRAIP directly interacts with PCNA via a conserved PIP box motif and localizes to active and stressed replication forks. TRAIP promotes ATR-dependent checkpoint signaling by facilitating RPA-bound single-stranded DNA generation upon replication stress; this requires both E3 ligase activity and the PIP box. Loss of TRAIP leads to chromosomal instability and reduced cell survival after replication stress. Co-immunoprecipitation; PIP box mutagenesis; RPA-ssDNA accumulation assays; ATR signaling readouts (Chk1 phosphorylation); clonogenic survival; chromosomal instability analysis The Journal of cell biology High 26711499
2015 TRAIP (encoding an E3 RING ubiquitin ligase) relocalizes to sites of DNA damage during S-phase and is required for optimal phosphorylation of H2AX and RPA2 in response to UV irradiation, as well as for replication fork progression through UV-induced lesions. Mutations in TRAIP cause microcephalic primordial dwarfism. Patient genetic analysis; laser microirradiation/foci formation; H2AX and RPA2 phosphorylation assays; DNA fiber assays for fork progression; siRNA knockdown Nature genetics High 26595769
2016 TRAIP is required for recruitment of RAP80 to DNA lesions; TRAIP acts upstream of RAP80 and downstream factors including BRCA1. The N-terminus of TRAIP mediates RAP80 interaction, while the C-terminus of TRAIP mediates localization to DNA damage sites through direct interaction with RNF20-RNF40. TRAIP thereby promotes homologous recombination. Co-immunoprecipitation; domain deletion analysis; laser microirradiation foci recruitment assays; siRNA knockdown; HR reporter assays Nature communications High 26781088
2016 TRAIP encodes a nucleolar protein that migrates to stalled replication forks by targeting PCNA via an evolutionarily conserved PIP box on its C-terminus. Inactivation of TRAIP or disruption of the TRAIP-PCNA interaction compromises replication fork recovery and progression and causes chromosome instability. GFP-TRAIP live imaging; PIP box mutagenesis; PCNA interaction assays; DNA fiber assays; chromosomal instability analysis Cell discovery Medium 27462463
2021 In mouse embryonic stem cells, TRAIP ubiquitin ligase mediates a second (mitotic) pathway of CMG helicase disassembly in addition to the S-phase CUL2LRR1-dependent pathway. Both CUL2LRR1 and TRAIP target CMG for ubiquitylation and p97-dependent disassembly, establishing that metazoan replisome disassembly is controlled by a conserved pair of ubiquitin ligases. Mouse embryonic stem cell genetic knockouts; chromatin fractionation; ubiquitylation assays; cell cycle staging EMBO reports High 33590678
2014 TRAIP localizes near mitotic chromosomes and its knockdown decreases early mitosis progression time and increases chromosome alignment defects and lagging chromosomes. TRAIP-depleted cells bypass taxol-induced mitotic arrest and show reduced kinetochore MAD2 levels, indicating that TRAIP E3 ubiquitin ligase activity is required for spindle assembly checkpoint control. RNAi knockdown in HeLa cells; live-cell imaging; flow cytometry; kinetochore immunofluorescence (MAD2); taxol/nocodazole treatment; rescue with wild-type vs. ubiquitin-ligase-dead TRAIP Journal of cell science Medium 25335891
2016 TRAIP is post-translationally modified by SUMOylation at five sites; SUMOylation is required for nuclear retention and protein stability of TRAIP. SUMOylation-deficient TRAIP fails to be retained in the nucleus and exhibits reduced protein stability. SUMOylation site mutagenesis; subcellular fractionation; immunofluorescence; protein stability assays (cycloheximide chase) Biochemical and biophysical research communications Medium 26820530
2018 PRRRSV non-structural protein 1α (nsp1α) interacts with TRAIP at K205, decreases TRAIP SUMOylation and K48-linked ubiquitination, causing redistribution of TRAIP from nucleus to cytoplasm. Cytoplasmic TRAIP then promotes K48-linked ubiquitination and degradation of TBK1, antagonizing TBK1-IRF3-IFN signaling. Co-immunoprecipitation; site-directed mutagenesis (K205); subcellular fractionation; TBK1 ubiquitination assays; IFN signaling reporter assays Frontiers in immunology Medium 30619364
2018 The TRAIP coiled-coil domain interacts with the TRAF-N domain of TRAF2 (but not TRAF1) to inhibit NF-κB signaling. The coiled-coil domain alters stoichiometry between dimer and trimer in a concentration-dependent manner, and the RING domain induces higher-ordered assembly required for TRAF2 interaction. In vitro binding/biochemical characterization; domain deletion and mutagenesis; size-exclusion chromatography; pull-down assays International journal of molecular sciences Medium 30127245
2018 TRAIP nucleolar residence is coupled to active ribosomal DNA transcription: RNA polymerase I inhibition or rDNA damage causes TRAIP to diffuse into the nucleoplasm. PCNA binding is dispensable for nucleolus-to-nucleoplasm shuttling after UV. Pre-treatment with DNase/RNase releases TRAIP from nucleoli, suggesting sequestration via nucleic acid structures. RNA polymerase I inhibition (CX-5461); I-PpoI-induced rDNA damage; DNase/RNase pre-treatment; live-cell imaging; PCNA PIP-box mutant analysis; ATM/ATR inhibition Nucleic acids research Medium 30165463
2015 TRAIP forms homodimers through its coiled-coil (CC) domain; expression of a CC-domain-deleted mutant that cannot homodimerize increases mitotic index and promotes mitotic progression, indicating that homodimerization is required for proper mitotic regulation. Co-immunoprecipitation with TRAIP deletion mutants; mitotic index measurements; flow cytometry Biochemical and biophysical research communications Medium 26093298
2019 TRAIP promotes ionizing radiation-induced H2B monoubiquitination at K120; deletion of the RING domain or C-terminus of TRAIP abolishes this activity, establishing a role for TRAIP in regulating this histone mark in the DNA damage response. siRNA knockdown; TRAIP deletion mutants; Western blot for H2B-K120 ubiquitination after IR Oncology reports Low 30942468
2023 TRAIP resolves replication-transcription conflicts specifically during S-phase; rapid TRAIP degradation in S-phase causes DNA damage at transcription start sites, G2 arrest, and cellular senescence. TRAIP's S-phase function in preventing replication-transcription conflicts is essential for cell proliferation. Auxin-induced degron system for rapid TRAIP degradation; cell cycle-specific depletion; γH2AX foci at transcription start sites; cell cycle analysis; senescence assays Nature communications High 37604812
2021 TRAIP promotes KANK1 polyubiquitination and subsequent proteasomal degradation, which downregulates IGFBP3 and activates AKT signaling in osteosarcoma cells. Co-immunoprecipitation; ubiquitination assays; Western blot for KANK1, IGFBP3, and AKT pathway; siRNA knockdown and overexpression Cell death & disease Low 34349117
2023 TRAIP directly interacts with MYC and promotes K48-linked polyubiquitination at MYC K428 and K430, leading to proteasome-dependent MYC degradation and suppression of MYC transcriptional activity in bladder cancer cells. MYC in turn binds the TRAIP promoter, establishing a negative feedback loop. Co-immunoprecipitation; ubiquitination assays with K428/K430 mutagenesis; proteasome inhibition; MYC transcriptional activity reporter; ChIP for MYC at TRAIP promoter; rescue experiments Oncogene Medium 38123820
2023 ZNF212 is a direct binding partner of TRAIP; mutual recruitment of TRAIP and ZNF212 to DNA damage sites is interdependent. ZNF212 depletion causes DDR and HR repair defects epistatic to TRAIP. In mESCs, Zfp212 acts upstream of both the NEIL3 and Fanconi anaemia ICL repair pathways. ZNF212 also directly interacts with NEIL3 and promotes its recruitment to ICL lesions. Co-immunoprecipitation (direct interaction); laser microirradiation foci recruitment; epistasis analysis in mESCs; ICL repair assays; NEIL3 recruitment assays Nucleic acids research Medium 36594163
2025 CDK phosphorylation of TRAIP in mitosis is essential for mitotic replisome unloading and MiDAS. TRAIP interacts with terminated replisomes in S-phase without ubiquitylating them; CDK phosphorylation specifically activates TRAIP's autoubiquitylation and ubiquitylation of replisomes isolated from mitotic chromatin. CDK-driven phosphorylation alone is not sufficient to activate replisome unloading during S-phase. Auxin-induced degron; CDK inhibition; phospho-mimetic/non-phosphorylatable TRAIP mutants; in vitro ubiquitylation assays with mitotic chromatin; MiDAS assays in human cells Nucleic acids research High 40637231
2025 USP37 deubiquitylase binds CMG helicase (via CDC45 contact) and counteracts TRAIP-dependent CMG ubiquitylation and premature replisome disassembly. In Xenopus extracts depleted of USP37, TRAIP promotes premature CMG ubiquitylation and disassembly when converging replisomes stall. TRAIP loss suppresses the hypersensitivity of USP37 knockout cells to topoisomerase inhibitors. Xenopus egg extract depletion; Usp37 knockout cells; genetic epistasis (TRAIP mutation suppressing USP37 KO phenotype); CMG ubiquitylation assays; structure-guided CDC45-binding mutations Nature communications High 40411782 40533495
2024 TRAIP phosphorylation by mitotic Cyclin B-CDK1 enables formation of a complex with TTF2 (a SWI/SNF ATPase) and DNA polymerase ε. TTF2 tethers TRAIP to replisomes via an N-terminal zinc finger that binds phosphorylated TRAIP and an adjacent peptide that contacts the CMG-associated leading strand DNA polymerase ε (Pol2/POLE2). This TRAIP-TTF2-Pol ε bridge is essential for CMG unloading and stalled fork breakage in mitosis, independently of TTF2 ATPase activity. Xenopus egg extract; AlphaFold-Multimer-guided mutagenesis; biochemical reconstitution; TTF2 zinc finger binding assays; CDK1 phosphorylation of TRAIP; CMG unloading assays; MiDAS assays bioRxivpreprint High bio_10.1101_2024.11.30.626186 bio_10.1101_2024.12.01.626218
2025 TRAIP depletion causes retention of PCNA on chromatin during mitosis; TRAIP-depleted cells entering G1 with chromatin-bound PCNA display reduced Cdt1 levels and impaired S-phase entry, and show delayed S-phase progression. TRAIP functions independently of ATAD5-RFC in PCNA unloading from chromatin. Auxin-induced degron for TRAIP depletion; chromatin fractionation; PCNA immunofluorescence; Cdt1 Western blot; S-phase entry assays; comparison with ATAD5-RFC-depleted cells Genes to cells Medium 39956965
2021 In vitro, the TRAIP coiled-coil domain (residues 64-166) specifically forms a complex with the RAP80 zinc finger motif (residues 490-584); larger TRAIP fragments (CCLZ or RINGCC) fail to interact with the RAP80 zinc finger under the same conditions. In vitro protein-protein interaction assay; SDS-PAGE; co-elution Saudi journal of biological sciences Low 34867056
2026 TRAIP functions as an E3 ubiquitin ligase that mediates K48-linked polyubiquitination and proteasomal degradation of PLSCR4 in breast cancer cells. Co-immunoprecipitation; K48-linked ubiquitination assays; rescue experiments with PLSCR4 knockdown Journal of biochemical and molecular toxicology Low 41942828
2026 SOX9 transcription factor directly binds the TRAIP promoter to drive its transcriptional upregulation. TRAIP then acts as the specific E3 ligase for CPEB3, mediating its poly-ubiquitylation and proteasomal degradation, which relieves CPEB3-mediated translational repression and hyperactivates mTORC1 signaling in gastric cancer cells. ChIP for SOX9 at TRAIP promoter; luciferase reporter assay; Co-immunoprecipitation; ubiquitylation assays; epistatic rescue experiments World journal of surgical oncology Low 41832516

Source papers

Stage 0 corpus · 38 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2019 TRAIP is a master regulator of DNA interstrand crosslink repair. Nature 151 30842657
2015 TRAIP promotes DNA damage response during genome replication and is mutated in primordial dwarfism. Nature genetics 79 26595769
2019 TRAIP drives replisome disassembly and mitotic DNA repair synthesis at sites of incomplete DNA replication. eLife 66 31545170
2015 TRAIP is a PCNA-binding ubiquitin ligase that protects genome stability after replication stress. The Journal of cell biology 62 26711499
2019 Mitotic replisome disassembly depends on TRAIP ubiquitin ligase activity. Life science alliance 44 30979826
2016 TRAIP/RNF206 is required for recruitment of RAP80 to sites of DNA damage. Nature communications 41 26781088
2016 TRAIP regulates replication fork recovery and progression via PCNA. Cell discovery 36 27462463
2021 CUL2LRR1 , TRAIP and p97 control CMG helicase disassembly in the mammalian cell cycle. EMBO reports 32 33590678
2021 TRAIP modulates the IGFBP3/AKT pathway to enhance the invasion and proliferation of osteosarcoma by promoting KANK1 degradation. Cell death & disease 32 34349117
2020 LncRNA SLC7A11-AS1 Contributes to Lung Cancer Progression Through Facilitating TRAIP Expression by Inhibiting miR-4775. OncoTargets and therapy 32 32636648
2020 The Ubiquitin Ligase TRAIP: Double-Edged Sword at the Replisome. Trends in cell biology 28 33317933
2014 TRAIP is a regulator of the spindle assembly checkpoint. Journal of cell science 26 25335891
2023 TRAIP resolves DNA replication-transcription conflicts during the S-phase of unperturbed cells. Nature communications 16 37604812
2019 Recognition of TRAIP with TRAFs: Current understanding and associated diseases. The international journal of biochemistry & cell biology 16 31442608
2015 Dimerization of TRAF-interacting protein (TRAIP) regulates the mitotic progression. Biochemical and biophysical research communications 14 26093298
2022 Silencing TRAIP suppresses cell proliferation and migration/invasion of triple negative breast cancer via RB-E2F signaling and EMT. Cancer gene therapy 13 36064576
2018 Dual Regulation of Host TRAIP Post-translation and Nuclear/Plasma Distribution by Porcine Reproductive and Respiratory Syndrome Virus Non-structural Protein 1α Promotes Viral Proliferation. Frontiers in immunology 12 30619364
2016 SUMOylation regulates nuclear localization and stability of TRAIP/RNF206. Biochemical and biophysical research communications 12 26820530
2023 TRAIP suppresses bladder cancer progression by catalyzing K48-linked polyubiquitination of MYC. Oncogene 11 38123820
2015 The TRAF-interacting protein (TRAIP) is a novel E2F target with peak expression in mitosis. Oncotarget 11 26369285
2016 TRAIP is involved in chromosome alignment and SAC regulation in mouse oocyte meiosis. Scientific reports 9 27405720
2020 Molecular cloning, expression, overproduction and characterization of human TRAIP Leucine zipper protein. Saudi journal of biological sciences 8 32489294
2019 TRAIP regulates Histone H2B monoubiquitination in DNA damage response pathways. Oncology reports 8 30942468
2018 In Vitro Inhibitory Mechanism Effect of TRAIP on the Function of TRAF2 Revealed by Characterization of Interaction Domains. International journal of molecular sciences 8 30127245
2018 Nucleolar residence of the seckel syndrome protein TRAIP is coupled to ribosomal DNA transcription. Nucleic acids research 7 30165463
2023 ZNF212 promotes genomic integrity through direct interaction with TRAIP. Nucleic acids research 5 36594163
2022 Traip controls mushroom body size by suppressing mitotic defects. Development (Cambridge, England) 5 35297981
2025 USP37 protects mammalian cells during DNA replication stress by counteracting CUL2LRR1 and TRAIP. Cell reports 4 40411782
2025 USP37 prevents premature disassembly of stressed replisomes by TRAIP. Nature communications 3 40533495
2024 USP37 prevents premature disassembly of stressed replisomes by TRAIP. bioRxiv : the preprint server for biology 3 39282314
2024 TRAIP enhances progression of tongue squamous cell carcinoma through EMT and Wnt/β-catenin signaling by interacting with DDX39A. BMC cancer 3 39623306
2025 CDK-driven phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS. Nucleic acids research 2 40637231
2023 TRAIP suppressed apoptosis and cell cycle to promote prostate cancer proliferation via TRAF2-PI3K-AKT pathway activation. International urology and nephrology 2 38100027
2026 Oncogenic SOX9-TRAIP signaling drives gastric cancer progression by mediating the degradation of the CPEB3-mTORC1 tumor suppressor axis. World journal of surgical oncology 0 41832516
2026 Loss of TRAIP Could Attenuate the Breast Cancer Cells Development by Regulating PLSCR4 Stabilization. Journal of biochemical and molecular toxicology 0 41942828
2025 The Depletion of TRAIP Results in the Retention of PCNA on Chromatin During Mitosis Leads to Inhibiting DNA Replication Initiation. Genes to cells : devoted to molecular & cellular mechanisms 0 39956965
2021 Prenatal ultrasound diagnosis of Seckel syndrome with bi-allelic variant in TRAIP via exome sequencing. Journal of clinical ultrasound : JCU 0 34235748
2021 In vitro assembly complex formation of TRAIP CC and RAP 80 zinc finger motif revealed by our study. Saudi journal of biological sciences 0 34867056

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