Affinage

TOM1

Target of Myb1 membrane trafficking protein · UniProt O60784

Length
492 aa
Mass
53.8 kDa
Annotated
2026-04-28
52 papers in source corpus 24 papers cited in narrative 24 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

TOM1 is a multidomain endosomal adaptor that couples ubiquitinated cargo recognition to clathrin-dependent endosomal sorting, autophagosome–lysosome fusion, and inflammatory signal attenuation. Its VHS domain binds phosphoinositides—particularly PI5P, which induces conformational destabilization and delays endosomal maturation—while its GAT domain binds ubiquitin and TOLLIP at an overlapping site; TOM1 suppresses TOLLIP's PI(3)P binding to commit cargo to the trafficking pathway, and its C-terminal region recruits clathrin and endosomal scaffolds such as endofin (PMID:15047686, PMID:15657082, PMID:25588840, PMID:40936361). TOM1 partners with myosin VI and autophagy receptors to promote autophagosome–lysosome fusion, and its loss impairs autophagic flux; accordingly, the disease-associated G307D variant disrupts TOM1–TOLLIP binding, causing autophagosome accumulation and excessive inflammatory signaling in patient cells (PMID:23023224, PMID:31263572, PMID:40936361). TOM1 also negatively regulates NF-κB and AP-1 activation downstream of IL-1β, TNF-α, and TLR signaling, a function dependent on its VHS domain and subject to post-translational regulation by miR-126, SUMOylation, and desuccinylation (PMID:15056867, PMID:20083669, PMID:37137262, PMID:39210272).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 1999 High

    Establishing that the yeast ortholog Tom1 is a HECT-domain E3 ubiquitin ligase resolved the gene's ancestral enzymatic function and linked it to nuclear division and nucleocytoplasmic transport.

    Evidence Active-site Cys mutagenesis abolishing ubiquitin thioester formation, electron microscopy in S. cerevisiae

    PMID:10395901

    Open questions at the time
    • No substrates identified at this stage
    • Human TOM1 lacks a HECT domain—ortholog relationship unclear
    • Mechanism of nuclear division defect unresolved
  2. 2000 High

    Solving the crystal structure of the human TOM1 VHS domain established that human TOM1 is structurally distinct from the yeast HECT ligase and provided a framework for mapping protein–protein and membrane interaction surfaces.

    Evidence X-ray crystallography at 1.5 Å resolution

    PMID:10985773

    Open questions at the time
    • Binding partners for the VHS domain not yet identified
    • No functional assays in cells performed
  3. 2003 High

    Demonstrating that TOM1 forms an endogenous complex with TOLLIP and ubiquitin, and recruits clathrin via C-terminal motifs, defined TOM1 as an endosomal sorting adaptor linking ubiquitinated cargo to clathrin machinery.

    Evidence Reciprocal Co-IP, gel filtration of endogenous complex, GST pulldown, fluorescence microscopy

    PMID:14563850 PMID:14613930

    Open questions at the time
    • GAT-domain binding site not yet mapped
    • Functional consequence for cargo trafficking not directly shown
  4. 2004 High

    Mapping ubiquitin and TOLLIP binding to overlapping sites on the GAT domain revealed a mutually exclusive switch governing cargo recognition versus adaptor recruitment, while reporter assays showed TOM1 negatively regulates NF-κB/AP-1 signaling.

    Evidence GST pulldown with mutagenesis, immunofluorescence localization, luciferase reporter assays

    PMID:15047686 PMID:15056867

    Open questions at the time
    • In vivo relevance of signaling suppression not tested
    • Mechanism by which VHS domain suppresses NF-κB unclear
  5. 2005 High

    Identifying three distinct clathrin-binding motifs in TOM1's C-terminus and showing that endofin uses TOM1 to recruit clathrin (but not dynamin or AP complexes) to endosomes established TOM1 as a selective endosomal clathrin adaptor.

    Evidence Site-directed mutagenesis of clathrin-binding motifs, antibody microinjection reducing membrane clathrin

    PMID:15657082

    Open questions at the time
    • Cargo sorted by this pathway not identified
    • Structural basis of clathrin engagement unknown
  6. 2010 High

    Showing that miR-126 targets TOM1 mRNA and that TOM1 knockdown amplifies NF-κB–driven IL-8 secretion upon LPS/IL-1β stimulation placed TOM1 as a physiologically relevant brake on innate immune signaling.

    Evidence 3′-UTR luciferase reporter, miRNA mimic, siRNA knockdown, ELISA for IL-8

    PMID:20083669

    Open questions at the time
    • Molecular mechanism of NF-κB suppression still undefined
    • In vivo immune phenotype of TOM1 loss not tested
  7. 2012 High

    Identifying TOM1 as a myosin VI partner required for autophagosome–lysosome fusion broadened TOM1's role from endosomal sorting to autophagy, while yeast studies identified Cdc6 and Dia2 as cell-cycle-regulated Tom1 substrates.

    Evidence siRNA knockdown with autophagy flux assays (human cells); ubiquitination assays, Co-IP, genetic epistasis (yeast)

    PMID:22933573 PMID:23023224 PMID:23129771

    Open questions at the time
    • Mechanism by which TOM1 facilitates membrane fusion unknown
    • Whether human TOM1 autophagy function depends on ubiquitin binding untested
  8. 2015 High

    Identifying TOM1 as a direct PI5P effector that delays EGFR degradation and inhibits fluid-phase endocytosis revealed a phosphoinositide-dependent mechanism for regulating endosomal maturation kinetics.

    Evidence Lipid-binding assays, EGFR degradation and endocytosis assays upon TOM1 knockdown/overexpression

    PMID:25588840

    Open questions at the time
    • PI5P-binding domain not fully mapped at this point
    • Pathological relevance of PI5P–TOM1 axis incompletely explored
  9. 2018 Medium

    Discovery that TOM1 binds FcγRIIb2 and regulates Aβ uptake in neurons, with TOM1 downregulation in AD brain and rescue of memory by TOM1 overexpression, linked TOM1 to neurodegeneration; meanwhile, yeast Tom1 was shown to target the FEAR network component Spo12 for G2/M degradation.

    Evidence Co-IP, lentiviral overexpression with behavioral rescue in 3xTg-AD mice; Co-IP and stability assay in yeast

    PMID:29683484 PMID:30185465

    Open questions at the time
    • AD association from single-lab mouse model
    • Direct ubiquitination of Spo12 by Tom1 not shown in vitro
  10. 2019 Medium

    The TOM1 G307D patient variant was shown to disrupt TOLLIP binding, impair autophagy, and compromise immune signaling, providing the first genetic evidence linking TOM1 dysfunction to human immunodeficiency; biophysical studies revealed PI5P binding destabilizes the VHS domain via two non-cooperative sites.

    Evidence Whole-exome sequencing, Co-IP of G307D variant, patient cell autophagy/signaling assays; ITC, NMR, DSF for PI5P binding

    PMID:31263572 PMID:31350523

    Open questions at the time
    • Single-family study for disease variant
    • In vivo validation of PI5P-induced conformational change lacking
    • Mechanism linking VHS destabilization to altered effector interactions unresolved
  11. 2024 Medium

    Post-translational regulation of TOM1 was expanded: SIRT5-mediated desuccinylation at K48 stabilizes TOM1 to promote autophagy, while a linker region enhancing ubiquitin binding is modulated by phosphorylation and pH, suggesting a pH-sensing mechanism for cargo commitment at acidifying endosomes.

    Evidence Succinylation assays, siRNA rescue in hypoxia/reoxygenation model; NMR and ITC pH-titration binding assays

    PMID:39208792 PMID:39210272

    Open questions at the time
    • Physiological kinase for linker phosphorylation unknown
    • In vivo relevance of pH-dependent ubiquitin release not established
    • SIRT5–TOM1 axis tested only in cardiac ischemia model
  12. 2025 High

    Biophysical confirmation that TOM1 suppresses TOLLIP PI(3)P binding to commit cargo to trafficking, combined with cryo-EM structures of yeast Tom1 revealing a structural ubiquitin coordinated by the solenoid domain to enforce K48 chain specificity, provided structural mechanisms for both the human adaptor and yeast E3 ligase functions.

    Evidence ITC/SPR for TOM1–TOLLIP–PI(3)P; cryo-EM of yeast Tom1 ubiquitination intermediates with mutagenesis

    PMID:40359109 PMID:40936361

    Open questions at the time
    • No high-resolution structure of full-length human TOM1
    • Structural ubiquitin concept not yet tested for in vivo chain specificity in yeast

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structural basis of full-length human TOM1 in complex with TOLLIP and cargo, the identity of the kinase(s) regulating TOM1 phosphorylation and pH-dependent cargo release, the precise mechanism by which TOM1 facilitates autophagosome–lysosome membrane fusion, and whether additional human disease-causing TOM1 variants exist.
  • No full-length human TOM1 structure
  • Kinase(s) responsible for linker phosphorylation unidentified
  • Membrane fusion mechanism unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 4 GO:0008289 lipid binding 3 GO:0098772 molecular function regulator activity 3
Localization
GO:0005768 endosome 4 GO:0005829 cytosol 2 GO:0031410 cytoplasmic vesicle 2
Pathway
R-HSA-5653656 Vesicle-mediated transport 4 R-HSA-9612973 Autophagy 4 R-HSA-162582 Signal Transduction 3 R-HSA-168256 Immune System 3
Partners
CALCOCO2CLTCFCGR2BMYO6SENP1SIRT5TOLLIPZFYVE16
Complex memberships
TOM1–TOLLIP complex

Evidence

Reading pass · 24 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 Crystal structure of the VHS domain of human TOM1 resolved to 1.5 Å, revealing eight helices in a superhelix with a basic patch on helix 3 and a negatively charged ridge on helix 2; conserved surfaces on helices 2 and 4 mediate protein-protein interactions, and the positively charged helix 3 surface is proposed to mediate membrane binding. X-ray crystallography (1.5 Å resolution) Biochemistry High 10985773
2003 TOM1 directly binds ubiquitin chains and Tollip, forming an endogenous complex (confirmed by gel filtration and Western blot); TOM1 also binds clathrin heavy chain through a clathrin-binding motif, and GFP-TOM1 localization in cytoplasm depends on this clathrin-binding motif. Co-immunoprecipitation, gel filtration, GST pulldown, fluorescence microscopy The Journal of biological chemistry High 14563850
2004 The GAT domain of TOM1 binds both ubiquitin and Tollip at an overlapping, mutually exclusive site; Tollip localizes to early endosomes and recruits cytosolic TOM1 and ubiquitinated proteins to endosomes; Tom1L1 shares these interactions. GST pulldown, co-immunoprecipitation, fluorescence microscopy, mutagenesis The Journal of biological chemistry High 15047686
2003 Endofin, a FYVE-domain early endosomal protein, directly binds the C-terminal region of TOM1 (confirmed by GST pulldown and co-IP); endofin overexpression recruits cytosolic TOM1 to endosomes, identifying TOM1 as an endofin effector. Yeast two-hybrid, GST pulldown, co-immunoprecipitation, immunofluorescence, sucrose gradient fractionation The Journal of biological chemistry High 14613930
2005 TOM1 recruits clathrin to early endosomes via its C-terminal clathrin-binding sites (residues 300–321, 321–326, and LEDEF motif at 362–366); endofin exploits this TOM1–clathrin interaction to specifically recruit clathrin (not dynamin or AP complexes) onto endosomes; microinjection of TOM1 antibody reduces membrane-associated clathrin. Mutagenesis, co-immunoprecipitation, overexpression, microinjection, immunofluorescence Journal of cell science High 15657082
2006 Tom1L1 and Tom1L2 also interact with Tollip via their GAT domains and recruit clathrin to endosomes via their C-terminal regions when coexpressed with Tollip, demonstrating a conserved function of the TOM1 family in clathrin recruitment to endosomes. Co-immunoprecipitation, fluorescence microscopy Biochemical and biophysical research communications Medium 16412388
2004 Tom1 overexpression suppresses NF-κB and AP-1 activation induced by IL-1β or TNF-α; the VHS domain is required for this suppressive activity, identifying TOM1 as a negative regulator of IL-1β– and TNF-α–induced signaling. Reporter gene (luciferase) assay, overexpression, domain deletion analysis Biological & pharmaceutical bulletin Medium 15056867
2012 Myosin VI, together with its adaptor proteins NDP52, optineurin, T6BP, and TOM1, is required for autophagosome maturation and autophagosome–lysosome fusion; TOM1 is identified as a myosin VI binding partner on endosomes, and loss of myosin VI or TOM1 blocks delivery of endocytic cargo to autophagosomes and prevents autophagosome–lysosome fusion. Co-immunoprecipitation, siRNA knockdown, fluorescence microscopy, autophagy flux assays Nature cell biology High 23023224
2010 miR-126 directly targets the 3′-UTR of TOM1 mRNA to suppress TOM1 protein levels; TOM1 overexpression downregulates NF-κB–driven IL-8 secretion, while TOM1 knockdown increases IL-8 secretion after LPS or IL-1β stimulation, placing TOM1 as a negative regulator of TLR2/4 and IL-1 signaling. Luciferase 3′-UTR reporter assay, miRNA mimic transfection, siRNA knockdown, NF-κB reporter assay, ELISA Journal of immunology High 20083669
2015 TOM1 directly binds phosphatidylinositol 5-monophosphate (PI5P); the interaction is mediated by a specific domain of TOM1; PI5P-dependent recruitment of TOM1 to signaling endosomes delays EGFR degradation and inhibits bulk fluid-phase endocytosis, identifying TOM1 as a PI5P effector that impedes endosomal maturation. Lipid-binding assays, protein-lipid overlay, siRNA knockdown, overexpression, EGFR degradation assay, endocytosis assay Journal of cell science High 25588840
1999 Yeast Tom1 encodes a ~380 kDa HECT-domain E3 ubiquitin ligase; the conserved cysteine in the HECT domain is required for ubiquitin thioester formation and for Tom1 function; loss of the HECT domain or the entire gene causes temperature-sensitive growth with defects in nuclear division, nucleolar integrity, and nucleocytoplasmic transport. Site-directed mutagenesis of catalytic Cys, ubiquitin conjugation assay (GST-HECT overexpression), electron microscopy, immunofluorescence, FISH Gene High 10395901
2012 Yeast HECT E3 ligase Tom1 is required for Cdc6 degradation during G1 phase of the cell cycle, acting independently of SCF(Cdc4); Tom1 directly immunoprecipitates Cdc6 via a C-terminal region of Cdc6; loss of Tom1 reduces Cdc6 ubiquitination and causes aberrant chromatin association of Cdc6 and Mcm4 in G1. Genetic epistasis, ubiquitination assay, co-immunoprecipitation, cell cycle analysis The Journal of biological chemistry High 23129771
2012 Yeast HECT E3 ligase Tom1 is required for cell-cycle-regulated degradation of the F-box protein Dia2 during G1 and G2/M; Tom1 binding to Dia2 is enhanced in G1 and reduced in S phase; Tom1 recognizes positively charged residues in the Dia2 degradation/NLS domain; loss of Dia2 partially suppresses tom1 temperature-sensitive growth and G1-to-S delay. Genetic epistasis, co-immunoprecipitation, cell cycle synchronization, ubiquitination assay, mutagenesis Molecular biology of the cell High 22933573
2018 Yeast HECT E3 ligase Tom1 directly interacts with and promotes the degradation of Spo12 (a FEAR network component) specifically in G2/M phase; overexpression of Spo12 is cytotoxic in the absence of Tom1. Co-immunoprecipitation, protein stability assay, genetic overexpression FEBS letters Medium 29683484
2017 Yeast Tom1 E3 ubiquitin ligase associates with aberrant nascent peptides targeted by the ribosome-bound quality control (RQC) complex; Tom1 interacts with the light (ribosome-unbound) RQC complex and limits accumulation and aggregation of aberrant peptides independently of its E3 ligase catalytic activity. Co-immunoprecipitation, genetic epistasis, protein aggregation assay, cell fractionation Molecular biology of the cell Medium 28298488
2025 Cryo-EM structures of yeast Tom1 during active ubiquitination reveal that a non-canonical ubiquitin-binding site in the solenoid domain coordinates a 'structural ubiquitin' that contributes to K48 poly-ubiquitin chain specificity; the extended domain architecture beyond the catalytic HECT module directly contributes to catalytic fidelity. Cryo-EM, in vitro ubiquitination assay, mutagenesis Cell reports High 40359109
2008 Dictyostelium DdTom1, the sole VHS-domain protein in Dictyostelium, has a GAT domain that binds ubiquitin and a C-terminal domain that recruits clathrin, EPS15, and TSG101 (an ESCRT-I component); both VHS and GAT domains bind phospholipids, enabling endosomal membrane recruitment; DdTom1 is proposed to constitute an ancestral ESCRT-0 complex. GST pulldown, co-immunoprecipitation, lipid-binding assays, fluorescence microscopy Traffic Medium 19054384
2018 TOM1 binds FcγRIIb2 and represses its recycling, thereby attenuating neuronal uptake of oligomeric Aβ1-42; TOM1 expression is downregulated in AD hippocampus and 3xTg-AD mice; lentiviral TOM1 overexpression rescues memory impairment in 3xTg-AD mice. Co-immunoprecipitation, knockdown/overexpression, lentiviral gene delivery, behavioral assays, KO mouse studies The Journal of neuroscience Medium 30185465
2019 The TOM1 G307D disease variant fails to interact with TOLLIP; patient-derived cells show impaired autophagy and enhanced susceptibility to apoptosis, reduced STAT and ERK1/2 signaling, and poor IFN-γ and IL-17 secretion, linking the TOM1–TOLLIP interaction to immune regulation. Whole-exome sequencing, co-immunoprecipitation, patient cell functional assays (autophagy flux, signaling, cytokine secretion) NPJ genomic medicine Medium 31263572
2019 The TOM1 VHS domain preferentially binds PI5P via two non-cooperative binding sites involving acyl chains; PI5P binding destabilizes the VHS domain structure (reduced thermostability, interhelical contacts, and compaction), mechanistically explaining how PI5P causes TOM1 to adopt a different conformational state that could alter downstream effector interactions. Thermal denaturation (DSF), isothermal calorimetry (ITC), NMR, CD spectroscopy Scientific reports Medium 31350523
2024 SIRT5 directly interacts with TOM1 and desuccinylates it at K48, stabilizing TOM1 protein; TOM1 knockdown reverses SIRT5-mediated promotion of autophagy and inhibition of apoptosis in a hypoxia/reoxygenation MIRI cell model, placing TOM1 downstream of SIRT5 in autophagy regulation. Co-immunoprecipitation, succinylation assay, siRNA knockdown, overexpression, autophagy assays, in vivo MI model BMC cardiovascular disorders Medium 39210272
2023 SENP1 alleviates CIH-induced neuroinflammation by de-SUMOylating TOM1, thereby stabilizing TOM1 and promoting microglial migration; SENP1 knockout enhances TOM1 SUMOylation, inhibits microglial migration, and worsens neuroinflammation and Aβ42 deposition. SENP1 overexpression/KO, SUMOylation assay, siRNA knockdown, in vitro and in vivo CIH models International immunopharmacology Medium 37137262
2025 The TOM1 G307D variant impairs TOM1–TOLLIP interaction; TOM1 normally reduces TOLLIP's PI(3)P binding (confirmed biophysically), committing TOLLIP to cargo trafficking; G307D patient cells show accumulated autophagosomes due to defective autophagosome–lysosome fusion and excessive activation of inflammatory pathways. Biophysical binding assays (ITC/SPR), co-immunoprecipitation, patient cell autophagy flux assays, lipid-binding assays Disease models & mechanisms High 40936361
2024 An internal linker region adjacent to the TOM1 VHS domain enhances ubiquitin-binding affinity and is modulated by phosphorylation; TOM1's PI5P binding is pH-dependent (as is TOLLIP's), and under acidic endosomal conditions TOM1 retains TOLLIP binding but loses ubiquitin binding, suggesting pH-sensing regulates cargo commitment versus PI5P-dependent signaling. NMR, ITC, mutagenesis, phosphorylation assays, pH-titration binding assays Structure Medium 39208792

Source papers

Stage 0 corpus · 52 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2012 Autophagy receptors link myosin VI to autophagosomes to mediate Tom1-dependent autophagosome maturation and fusion with the lysosome. Nature cell biology 240 23023224
2010 miR-126 is downregulated in cystic fibrosis airway epithelial cells and regulates TOM1 expression. Journal of immunology (Baltimore, Md. : 1950) 163 20083669
2000 TOM1, an Arabidopsis gene required for efficient multiplication of a tobamovirus, encodes a putative transmembrane protein. Proceedings of the National Academy of Sciences of the United States of America 142 10944200
2004 Tollip and Tom1 form a complex and recruit ubiquitin-conjugated proteins onto early endosomes. The Journal of biological chemistry 128 15047686
2003 Tom1, a VHS domain-containing protein, interacts with tollip, ubiquitin, and clathrin. The Journal of biological chemistry 115 14563850
2003 Arabidopsis TOBAMOVIRUS MULTIPLICATION (TOM) 2 locus encodes a transmembrane protein that interacts with TOM1. The EMBO journal 72 12514139
1987 Establishment and characterization of a cell line, TOM-1, derived from a patient with Philadelphia chromosome-positive acute lymphocytic leukemia. Blood 68 3103721
2000 Structure of the VHS domain of human Tom1 (target of myb 1): insights into interactions with proteins and membranes. Biochemistry 61 10985773
1993 Effects of the tom1 mutation of Arabidopsis thaliana on the multiplication of tobacco mosaic virus RNA in protoplasts. Journal of virology 61 8350399
1997 tom-1, a novel v-Myb target gene expressed in AMV- and E26-transformed myelomonocytic cells. The EMBO journal 59 9135152
2015 TOM1 is a PI5P effector involved in the regulation of endosomal maturation. Journal of cell science 53 25588840
2006 Recruitment of clathrin onto endosomes by the Tom1-Tollip complex. Biochemical and biophysical research communications 50 16412388
2008 Dictyostelium Tom1 participates to an ancestral ESCRT-0 complex. Traffic (Copenhagen, Denmark) 48 19054384
2004 Tom1 (target of Myb 1) is a novel negative regulator of interleukin-1- and tumor necrosis factor-induced signaling pathways. Biological & pharmaceutical bulletin 37 15056867
2003 Endofin recruits TOM1 to endosomes. The Journal of biological chemistry 32 14613930
2005 Endofin recruits clathrin to early endosomes via TOM1. Journal of cell science 31 15657082
1999 Yeast tom1 mutant exhibits pleiotropic defects in nuclear division, maintenance of nuclear structure and nucleocytoplasmic transport at high temperatures. Gene 31 10395901
2008 Overexpression of a host factor TOM1 inhibits tomato mosaic virus propagation and suppression of RNA silencing. Virology 28 18440043
2019 Amyloid-beta impairs TOM1-mediated IL-1R1 signaling. Proceedings of the National Academy of Sciences of the United States of America 24 31570577
2018 TOM1 Regulates Neuronal Accumulation of Amyloid-β Oligomers by FcγRIIb2 Variant in Alzheimer's Disease. The Journal of neuroscience : the official journal of the Society for Neuroscience 24 30185465
2013 [miR-126 inhibits colon cancer proliferation and invasion through targeting IRS1, SLC7A5 and TOM1 gene]. Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 24 23981989
1995 A high dose of the STM1 gene suppresses the temperature sensitivity of the tom1 and htr1 mutants in Saccharomyces cerevisiae. Biochimica et biophysica acta 24 7548221
2021 Protein Trafficking or Cell Signaling: A Dilemma for the Adaptor Protein TOM1. Frontiers in cell and developmental biology 20 33718385
2012 The Hect domain E3 ligase Tom1 and the F-box protein Dia2 control Cdc6 degradation in G1 phase. The Journal of biological chemistry 20 23129771
1999 TOM1 genes map to human chromosome 22q13.1 and mouse chromosome 8C1 and encode proteins similar to the endosomal proteins HGS and STAM. Genomics 20 10329004
2023 SENP1 modulates chronic intermittent hypoxia-induced inflammation of microglia and neuronal injury by inhibiting TOM1 pathway. International immunopharmacology 18 37137262
2008 Gene-based SNP mapping of a psychotic bipolar affective disorder linkage region on 22q12.3: association with HMG2L1 and TOM1. American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics 18 17671966
2006 Involvement of THH1, an Arabidopsis thaliana homologue of the TOM1 gene, in tobamovirus multiplication. The Journal of general virology 17 16847136
2019 Dominant TOM1 mutation associated with combined immunodeficiency and autoimmune disease. NPJ genomic medicine 15 31263572
2012 Hect E3 ubiquitin ligase Tom1 controls Dia2 degradation during the cell cycle. Molecular biology of the cell 14 22933573
2001 Genes encoding ribosomal proteins Rps0A/B of Saccharomyces cerevisiae interact with TOM1 mutants defective in ribosome synthesis. Genetics 14 11238398
2000 Extragenic suppressors that rescue defects in the heat stress response of the budding yeast mutant tom1. Molecular & general genetics : MGG 14 10660055
2024 SIRT5 induces autophagy and alleviates myocardial infarction via desuccinylation of TOM1. BMC cardiovascular disorders 13 39210272
2007 A TOM1 homologue is required for multiplication of Tobacco mosaic virus in Nicotiana benthamiana. Journal of Zhejiang University. Science. B 13 17444600
2000 The yeast peptidyl proline isomerases FPR3 and FPR4, in high copy numbers, suppress defects resulting from the absence of the E3 ubiquitin ligase TOM1. Molecular & general genetics : MGG 13 10821187
1999 Myb and Ets transcription factors cooperate at the myb-inducible promoter of the tom-1 gene. Biochimica et biophysica acta 13 10524199
2023 Editing of TOM1 gene in tobacco using CRISPR/Cas9 confers resistance to Tobacco mosaic virus. Molecular biology reports 12 37119416
2017 The ribosome-bound quality control complex remains associated to aberrant peptides during their proteasomal targeting and interacts with Tom1 to limit protein aggregation. Molecular biology of the cell 12 28298488
2016 Immunolocalization of Tom1 in relation to protein degradation systems in Alzheimer's disease. Journal of the neurological sciences 9 27206884
2012 Inhibition of TMV multiplication by siRNA constructs against TOM1 and TOM3 genes of Capsicum annuum. Journal of virological methods 8 22814091
2018 Conferring virus resistance in tomato by independent RNA silencing of three tomato homologs of Arabidopsis TOM1. Archives of virology 7 29411138
2019 Preferential phosphatidylinositol 5-phosphate binding contributes to a destabilization of the VHS domain structure of Tom1. Scientific reports 5 31350523
2018 The HECT-type ubiquitin ligase Tom1 contributes to the turnover of Spo12, a component of the FEAR network, in G2/M phase. FEBS letters 4 29683484
2016 Structure of the GAT domain of the endosomal adapter protein Tom1. Data in brief 4 26977434
2025 Structural ubiquitin contributes to K48 linkage specificity of the HECT ligase Tom1. Cell reports 3 40359109
2022 The Penicillium chrysogenum tom1 Gene a Major Target of Transcription Factor MAT1-1-1 Encodes a Nuclear Protein Involved in Sporulation. Frontiers in fungal biology 3 37746180
2016 Backbone 1H, 15N, and 13C resonance assignments of the Tom1 VHS domain. Biomolecular NMR assignments 3 27704363
2023 TOM1 family conservation within the plant kingdom for tobacco mosaic virus accumulation. Molecular plant pathology 2 37443447
2025 A TOM1 variant impairs interaction with TOLLIP, autophagosome-lysosome fusion and regulation of innate immunity. Disease models & mechanisms 1 40936361
2024 An internal linker and pH biosensing by phosphatidylinositol 5-phosphate regulate the function of the ESCRT-0 component TOM1. Structure (London, England : 1993) 1 39208792
2015 Time to Fold: Tom1 Uses New Tricks to Regulate Lipid Binding of Tollip. Structure (London, England : 1993) 1 26445490
2025 Herb pair of Astragali Radix-Descurainiae Semen attenuate heart failure through the myosin VI-Tom1 complex mediated autophagy. Frontiers in cardiovascular medicine 0 40687556