Affinage

TOM1

Target of Myb1 membrane trafficking protein · UniProt O60784

Length
492 aa
Mass
53.8 kDa
Annotated
2026-06-10
49 papers in source corpus 27 papers cited in narrative 27 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

Human TOM1 is a VHS- and GAT-domain endosomal adaptor that sorts ubiquitinated cargo and couples endosomal membranes to downstream degradative machinery (PMID:14563850, PMID:15047686). Its GAT domain binds ubiquitin chains and Tollip through overlapping, mutually exclusive interfaces, and Tollip — together with the FYVE-domain protein endofin, which binds TOM1's C-terminal region — recruits cytosolic TOM1 onto early endosomes (PMID:15047686, PMID:14613930). Once membrane-bound, TOM1 engages clathrin heavy chain through three C-terminal binding sites including a LEDEF clathrin box, recruiting clathrin (but not AP1/AP2/AP3 or dynamin) to endosomes (PMID:14563850, PMID:15657082). TOM1 also binds myosin VI on endosomes, connecting endosomal membranes to autophagosomes and driving autophagosome–lysosome fusion (PMID:23023224). Phosphatidylinositol 5-phosphate is a direct TOM1 ligand: PI5P binding by the VHS domain — exploited during Shigella infection — destabilizes the VHS fold and, in a pH-dependent manner, diverts TOM1 from ubiquitin binding while preserving Tollip complex formation, delaying endosomal maturation and cargo degradation (PMID:25588840, PMID:31350523, PMID:39208792). TOM1 negatively regulates IL-1β/TNF-α-induced NF-κB and AP-1 signaling via its VHS domain (PMID:15056867), and its abundance is controlled post-translationally by SUMOylation reversed by SENP1 (PMID:37137262) and by K48 succinylation reversed by SIRT5 (PMID:39210272). A TOM1 p.G307D missense variant abolishes the TOLLIP interaction, impairs autophagosome–lysosome fusion, and causes excessive inflammatory signaling in patient cells, linking TOM1 dysfunction to human disease (PMID:31263572, PMID:40936361). The yeast ortholog Tom1 is a mechanistically distinct ~380 kDa HECT-domain E3 ubiquitin ligase whose solenoid architecture coordinates a structural ubiquitin to enforce K48-linked chain specificity and which ubiquitinates Cdc6, Dia2, and Spo12 to govern cell-cycle progression and protein quality control (PMID:10395901, PMID:40359109, PMID:23129771, PMID:22933573).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 1997 High

    Established how TOM1 expression is driven, identifying it as a transcriptional output of an oncogenic transcription factor rather than a constitutive housekeeping gene.

    Evidence Promoter deletion/mutation and reporter assays mapping a functional Myb site cooperating with C/EBP

    PMID:9135152

    Open questions at the time
    • Does not address TOM1 protein function
    • Physiological contexts of Myb-driven induction not defined
  2. 1999 High

    Defined the yeast ortholog as a catalytically active HECT E3 ubiquitin ligase, establishing an enzymatic identity entirely distinct from the human adaptor.

    Evidence Active-site cysteine mutagenesis (C3235A) and in vivo ubiquitin conjugation assay with GST-HECT overproduction in yeast

    PMID:10395901

    Open questions at the time
    • Substrates not yet identified
    • No structural basis for chain specificity
  3. 2000 High

    Provided the first atomic view of the TOM1 VHS domain, distinguishing membrane-binding and protein-interaction surfaces that later work would tie to function.

    Evidence X-ray crystallography at 1.5 Å

    PMID:10985773

    Open questions at the time
    • No bound ligand in the structure
    • Functional roles of the basic patch and charged ridge inferred, not tested here
  4. 2004 High

    Resolved how TOM1 recognizes cargo and is recruited to membranes, showing GAT-domain binding of ubiquitin and Tollip is mutually exclusive and that Tollip and endofin deliver cytosolic TOM1 to early endosomes.

    Evidence GST pulldown, reciprocal Co-IP, gel filtration, domain mapping and EEA1 co-localization microscopy across multiple studies

    PMID:14563850 PMID:14613930 PMID:15047686

    Open questions at the time
    • Stoichiometry of the TOM1–Tollip–ubiquitin switch unresolved
    • Spatial regulation of mutual exclusivity in vivo not defined
  5. 2005 High

    Mapped the clathrin-recruitment activity of TOM1 to defined C-terminal motifs and showed it selectively brings clathrin (not adaptor complexes or dynamin) to endosomes.

    Evidence GST pulldown with deletion/point mutants, overexpression, immunofluorescence and anti-TOM1 antibody microinjection; family-wide confirmation by Co-IP

    PMID:15657082 PMID:16412388

    Open questions at the time
    • Functional consequence of endosomal clathrin recruitment not fully defined
    • Family member redundancy in vivo untested
  6. 2004 Medium

    Linked TOM1 to inflammatory signaling control, showing it dampens cytokine-induced NF-κB/AP-1 activation through its VHS domain.

    Evidence NF-κB and AP-1 luciferase reporter assays with domain deletion in overexpression

    PMID:15056867

    Open questions at the time
    • No endogenous knockdown in original study
    • Molecular target of VHS-mediated suppression not identified
  7. 2010 Medium

    Identified post-transcriptional control of TOM1 by miR-126 and tied TOM1 levels causally to NF-κB-driven cytokine output.

    Evidence 3'-UTR luciferase reporter, siRNA knockdown, overexpression and IL-8 ELISA in bronchial epithelial cells

    PMID:20083669

    Open questions at the time
    • Single cell-type context
    • Direct molecular mechanism linking TOM1 to NF-κB not resolved
  8. 2012 High

    Placed TOM1 at the endosome–autophagosome interface, showing myosin VI binding is required for autophagosome maturation and lysosomal fusion.

    Evidence Reciprocal Co-IP, siRNA knockdown, live-cell imaging and autophagy flux assays

    PMID:23023224

    Open questions at the time
    • How TOM1 physically bridges the two membranes mechanistically unresolved
    • Regulation of this step not defined
  9. 2012 High

    Defined yeast Tom1 substrates and its role in cell-cycle control, showing ubiquitination of Cdc6 and Dia2 governs G1-to-S progression and replication licensing.

    Evidence Co-IP of substrates, in vitro/in vivo ubiquitination assays, chromatin fractionation, recognition-motif mutagenesis and genetic epistasis

    PMID:22933573 PMID:23129771

    Open questions at the time
    • Full substrate repertoire incomplete
    • Redundant E3s for these substrates exist
  10. 2015 High

    Identified PI5P as a direct TOM1 ligand exploited by Shigella, showing lipid-driven recruitment to signaling endosomes blocks cargo degradation and maturation.

    Evidence Lipid pulldown, Co-IP, domain mapping and cell-based EGFR degradation/endocytosis assays

    PMID:25588840

    Open questions at the time
    • Endogenous physiological role of PI5P sensing beyond infection unclear
    • Downstream effectors diverted by PI5P not enumerated
  11. 2016 Medium

    Provided a solution structure of the GAT domain, revealing conformational differences between Tollip-bound and ubiquitin-bound states underlying the mutually exclusive switch.

    Evidence Solution NMR structural determination

    PMID:26977434

    Open questions at the time
    • Limited functional validation in this data article
    • Switch dynamics in a membrane context untested
  12. 2017 Medium

    Extended yeast Tom1 into protein quality control, showing it limits aggregation of stalled RQC peptides through a ligase-independent function.

    Evidence Co-IP, genetic deletion, aggregation assays and a ligase-dead mutant

    PMID:28298488

    Open questions at the time
    • Mechanism of catalysis-independent aggregate prevention unknown
    • Single-lab observation
  13. 2018 Medium

    Added Spo12 as a yeast Tom1 substrate in G2/M and broadened TOM1's reach to mammalian receptor handling and Alzheimer-relevant amyloid uptake.

    Evidence Co-IP, genetic deletion and stability assays in yeast; Co-IP, Fcgr2b-knockout neurons, lentiviral rescue and behavior in mouse AD models

    PMID:29683484 PMID:30185465

    Open questions at the time
    • Additional Spo12 E3 ligases remain active
    • Mechanistic link between FcγRIIb2 binding and Aβ uptake incompletely defined
  14. 2019 High

    Characterized the biophysics of PI5P sensing and linked a human disease variant to loss of the TOLLIP interaction, autophagy defects and apoptosis susceptibility.

    Evidence ITC, thermal denaturation and NMR for PI5P-induced VHS destabilization; whole-exome sequencing, Co-IP and patient-cell autophagy/signaling assays for the G307D variant

    PMID:31263572 PMID:31350523

    Open questions at the time
    • Causality of the disease variant in a defined Mendelian syndrome not fully established
    • Effectors altered by VHS destabilization unidentified
  15. 2023 Medium

    Defined SUMOylation, reversed by SENP1, as a stability switch placing TOM1 downstream of SENP1 in controlling microglial migration and neuroinflammation.

    Evidence SUMO-IP, siRNA knockdown rescue, SENP1 gain/loss in CIH cell and animal models

    PMID:37137262

    Open questions at the time
    • SUMO acceptor site(s) not mapped
    • Single disease-model context
  16. 2024 Medium

    Established succinylation at K48 (reversed by SIRT5) as a second stability-regulating modification and refined the pH-dependent logic separating TOM1 ubiquitin binding from TOLLIP binding.

    Evidence SIRT5 Co-IP and succinylation-IP with knockdown rescue in MIRI models; biophysical, phosphorylation and pH-dependent binding assays plus Co-IP

    PMID:39208792 PMID:39210272

    Open questions at the time
    • Interplay between SUMOylation and succinylation unknown
    • Physiological pH ranges driving the ubiquitin/TOLLIP switch in vivo not defined
  17. 2025 High

    Delivered a structural mechanism for yeast Tom1 chain fidelity and a refined mechanism for the human disease variant's autophagy and inflammatory defects.

    Evidence Cryo-EM of an active ubiquitination cycle with ubiquitin-binding-site mutagenesis (yeast); biophysical binding, Co-IP and patient-cell autophagy/inflammation assays for G307D and PI(3)P regulation

    PMID:40359109 PMID:40936361

    Open questions at the time
    • Whether a structural ubiquitin contributes to other HECT ligases untested here
    • How G307D-driven PI(3)P dysregulation feeds into inflammation mechanistically incomplete
  18. 2026 Medium

    Uncovered a new TOM1 function in stress granule dynamics, showing G3BP1 binding promotes SG disassembly and drives an oncogenic transcriptional program in colitis-associated tumorigenesis.

    Evidence Co-IP, overexpression, nuclear fractionation and SG assembly/disassembly assays with FUBP1/c-Myc readouts

    PMID:42127552

    Open questions at the time
    • Direct vs. indirect effect on SG disassembly not fully separated
    • How this function integrates with endosomal/autophagy roles unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unresolved how TOM1's distinct activities — endosomal cargo sorting, autophagosome maturation, inflammatory suppression, stress granule disassembly — are coordinated and switched by its PI5P/pH sensing and competing post-translational modifications within a single cell.
  • No integrated model linking PTM state to functional partitioning
  • Physiological triggers selecting between TOM1 functions undefined
  • Stoichiometry and dynamics of competing partner interactions in vivo unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016874 ligase activity 4 GO:0060090 molecular adaptor activity 4 GO:0008289 lipid binding 3 GO:0098772 molecular function regulator activity 1
Localization
GO:0005768 endosome 4 GO:0005829 cytosol 2
Pathway
R-HSA-1640170 Cell Cycle 3 R-HSA-392499 Metabolism of proteins 3 R-HSA-5653656 Vesicle-mediated transport 3 R-HSA-9612973 Autophagy 3 R-HSA-168256 Immune System 2
Partners
CDC6CLTCDIA2FCGR2BG3BP1MYO6TOLLIPZFYVE16
Complex memberships
ESCRT-0 (TOM1–Tollip)

Evidence

Reading pass · 27 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2000 Crystal structure of the human TOM1 VHS domain resolved to 1.5 Å, revealing eight helices in a superhelix with a conserved basic patch on helix 3 (implicated in membrane binding) and a negatively charged ridge on helix 2 (implicated in protein-protein interactions involving helices 2 and 4). X-ray crystallography (1.5 Å resolution) Biochemistry High 10985773
1997 TOM1 is a direct transcriptional target of v-Myb; the Myb-inducible tom-1 promoter contains a functional Myb binding site and requires a juxtaposed C/EBP binding site for Myb-dependent activation, with C/EBP acting as a cooperation partner. Promoter deletion/mutation analysis, reporter gene assay, transcription factor binding site mapping The EMBO journal High 9135152
2003 TOM1 directly binds ubiquitin chains and Tollip via its GAT domain; endogenous TOM1 co-fractionates with Tollip in a complex; TOM1 also binds clathrin heavy chain through a canonical clathrin-binding motif; deletion of the clathrin-binding motif alters GFP-TOM1 cytoplasmic distribution. Co-immunoprecipitation, gel filtration, Western blot, GFP fluorescence microscopy, GST pulldown The Journal of biological chemistry High 14563850
2004 The GAT domain of TOM1 (and TOM1L1) interacts with ubiquitin and Tollip; ubiquitin and Tollip bind overlapping regions of the GAT domain in a mutually exclusive manner. Tollip localizes on early endosomes and recruits cytosolic TOM1 and ubiquitinated proteins to endosomes. GST pulldown, Co-immunoprecipitation, fluorescence microscopy, domain mapping The Journal of biological chemistry High 15047686
2003 Endofin, a FYVE-domain early endosomal protein, binds specifically to the C-terminal region of TOM1 (not TOM1-L1 or TOM1-L2) and recruits cytosolic TOM1 to early endosomes; confirmed by yeast two-hybrid, GST pulldown, Co-IP, and immunofluorescence with EEA1 co-localization. Yeast two-hybrid screen, GST pulldown, Co-immunoprecipitation, immunofluorescence, sucrose density gradient fractionation The Journal of biological chemistry High 14613930
2005 TOM1 binds clathrin heavy chain through three sites (residues 300–321, 321–326, and a LEDEF clathrin-binding box at 362–366); endofin overexpression recruits clathrin (but not dynamin, AP1, AP2, or AP3) to endosomes via TOM1; microinjection of anti-TOM1 antibody reduces membrane association of clathrin. GST pulldown with deletion/point mutants, overexpression, immunofluorescence, antibody microinjection Journal of cell science High 15657082
2006 All TOM1 family members (TOM1, TOM1L1, TOM1L2) bind Tollip via their GAT domains and bind clathrin through their C-terminal regions; when co-expressed with Tollip, all three family members recruit clathrin onto endosomes. Co-immunoprecipitation, fluorescence microscopy with Tollip co-expression Biochemical and biophysical research communications Medium 16412388
2004 Overexpression of TOM1 suppresses NF-κB and AP-1 activation induced by IL-1β and TNF-α; the VHS domain of TOM1 is required for this suppressive activity. Reporter gene assay (NF-κB and AP-1 luciferase), domain deletion analysis Biological & pharmaceutical bulletin Medium 15056867
2008 Dictyostelium TOM1 (DdTom1) GAT domain binds ubiquitin; the VHS and GAT domains interact with phospholipids enabling endosomal membrane recruitment; the C-terminal domain recruits clathrin, Eps15, and TSG101 (an ESCRT component), supporting participation in an ancestral ESCRT-0 complex for sorting ubiquitinated proteins to MVBs. Domain-binding assays, subcellular fractionation, co-immunoprecipitation, fluorescence microscopy Traffic (Copenhagen, Denmark) Medium 19054384
2010 miR-126 directly targets the 3'-UTR of TOM1 mRNA; introduction of synthetic pre-miR-126 in CF bronchial epithelial cells inhibited luciferase activity from a TOM1 3'-UTR reporter and decreased TOM1 protein; TOM1 knockdown increased NF-κB-regulated IL-8 secretion, while TOM1 overexpression downregulated NF-κB activity after LPS or IL-1β stimulation. Luciferase 3'-UTR reporter assay, Western blot, siRNA knockdown, cytokine ELISA Journal of immunology Medium 20083669
2012 TOM1 is a myosin VI binding partner on endosomes; loss of myosin VI and TOM1 reduces autophagosomal delivery of endocytic cargo and causes a block in autophagosome-lysosome fusion; myosin VI adaptor proteins NDP52, optineurin, and T6BP dock myosin VI to autophagosomes, while TOM1 on endosomes connects endosomal membranes to autophagosomes to promote maturation and lysosomal fusion. Co-immunoprecipitation, siRNA knockdown, live-cell fluorescence imaging, autophagy flux assays Nature cell biology High 23023224
2015 TOM1 is a direct binding partner of phosphatidylinositol 5-phosphate (PI5P); PI5P recruits TOM1 to signaling endosomes (produced by Shigella IpgD phosphatase), and this recruitment delays EGFR degradation and impairs bulk endocytosis, thereby preventing endosomal maturation. Lipid pulldown, Co-immunoprecipitation, domain mapping, cell-based endocytosis/degradation assays Journal of cell science High 25588840
1999 Yeast TOM1 encodes a ~380 kDa HECT-domain ubiquitin ligase; site-directed mutagenesis of the conserved active-site cysteine (C3235A) abolishes HECT function and temperature-sensitive growth; overproduction of the HECT domain promotes ubiquitin (but not SUMO/Smt3) conjugation to proteins in vivo. Site-directed mutagenesis, in vivo ubiquitin conjugation assay with GST-HECT overproduction, genetic complementation Gene High 10395901
2012 Yeast HECT E3 ligase Tom1 and F-box protein Dia2 independently control Cdc6 degradation in G1 phase; both Tom1 and Dia2 immunoprecipitate Cdc6 (binding its C-terminal region) and are required for Cdc6 ubiquitination; Cdc6 and Mcm4 chromatin association is aberrant in tom1Δ cells in G1. Co-immunoprecipitation, ubiquitination assay, chromatin fractionation, genetic deletion The Journal of biological chemistry High 23129771
2012 Yeast HECT E3 ligase Tom1 ubiquitinates the F-box protein Dia2 for its cell-cycle-dependent degradation in G1 and G2/M (stabilized in S phase); Tom1 binding to Dia2 is enhanced in G1 and reduced in S phase; Tom1 recognizes specific positively charged residues in the Dia2 degradation/NLS domain; loss of Dia2 partially suppresses tom1 temperature-sensitive phenotype; Dia2 degradation is required for G1-to-S phase progression. Genetic deletion, Co-immunoprecipitation, ubiquitination assay, cell cycle analysis, domain point mutagenesis Molecular biology of the cell High 22933573
2016 Solution NMR structure of the TOM1 GAT domain determined, showing a bundle of three helical elements; structural comparison with Tollip TBD-bound and ubiquitin-bound states reveals conformational differences at the binding interface. Solution NMR structural determination Data in brief Medium 26977434
2017 Yeast Tom1 E3 ubiquitin ligase interacts with aberrant nascent peptides escorted by the ribosome-bound quality control (RQC) complex to the proteasome; Tom1 limits accumulation and aggregation of stalled peptides in rqc1Δ cells; notably, Tom1's E3 ubiquitin ligase activity is not required for this aggregate-prevention function. Co-immunoprecipitation, genetic deletion, protein aggregation assay Molecular biology of the cell Medium 28298488
2018 Yeast HECT E3 ligase Tom1 physically interacts with Spo12 (a FEAR network component) and mediates its ubiquitin-dependent turnover in G2/M phase; overexpression of Spo12 is cytotoxic in tom1Δ; Spo12 is still degraded in S phase in the absence of Tom1 and Cdh1, indicating additional E3 ligase(s). Co-immunoprecipitation, genetic deletion, protein stability assay FEBS letters Medium 29683484
2018 TOM1 binds FcγRIIb2 (a variant Fc receptor) and attenuates its recycling, thereby suppressing neuronal uptake of oligomeric Aβ1-42; TOM1 expression is downregulated in the hippocampus of 3xTg-AD mice and AD patients; lentiviral TOM1 restoration rescues memory deficits in 3xTg-AD mice. Co-immunoprecipitation, Fcgr2b knockout neurons, lentiviral gene delivery, behavioral testing The Journal of neuroscience Medium 30185465
2019 TOM1 VHS domain preferentially binds PI5P over other phosphoinositides; PI5P binding destabilizes the VHS domain structure (reduced thermostability, interhelical contacts, and conformational compaction) and binding is endothermic with two non-cooperative binding sites; acyl chains of PI5P contribute to interaction; this structural change may alter downstream effector interactions under Shigella infection. Thermal denaturation (DSF), isothermal calorimetry (ITC), NMR spectroscopy Scientific reports High 31350523
2019 The TOM1 p.G307D missense variant fails to interact with TOLLIP; patient-derived cells show impaired autophagy and enhanced apoptosis susceptibility; these functional defects are consistent with TOM1's established role in TOLLIP-dependent IL-1 recycling and autophagosome maturation. Whole-exome sequencing, Co-immunoprecipitation (mutant vs. WT), autophagy flux assays in patient cells, signaling assays (STAT, ERK1/2) NPJ genomic medicine Medium 31263572
2023 SENP1 de-SUMOylates TOM1, promoting its stability and function; CIH induces SUMOylation of TOM1 leading to its destabilization; SENP1 overexpression reduces TOM1 SUMOylation, enhances TOM1 levels and microglial migration, and reduces neuroinflammation; TOM1 knockdown suppresses these SENP1-driven effects, placing TOM1 downstream of SENP1 in this pathway. Co-immunoprecipitation, siRNA knockdown, SENP1 overexpression/knockout, SUMO immunoprecipitation, in vitro and in vivo CIH model International immunopharmacology Medium 37137262
2024 SIRT5 directly interacts with TOM1 and desuccinylates it at the K48 site, stabilizing TOM1 protein; TOM1 knockdown reverses the pro-autophagy and anti-apoptotic effects of SIRT5 overexpression in an H/R-induced MIRI cell model, placing TOM1 downstream of SIRT5 in regulating autophagy-related cell death. Co-immunoprecipitation, succinylation immunoprecipitation, siRNA knockdown rescue assay, overexpression, in vivo MI model BMC cardiovascular disorders Medium 39210272
2024 TOM1 contains a DXXLL motif-adjacent region that enhances VHS domain affinity for ubiquitin and is modulated by phosphorylation; TOM1 PI5P binding is pH-dependent—under acidic conditions TOM1 retains TOLLIP complex formation but cannot bind ubiquitin; acidic endosomal pH promotes TOM1 and TOLLIP accumulation at endosomes through this pH-sensing mechanism. Biophysical binding assays, phosphorylation analysis, pH-dependent binding assays, Co-immunoprecipitation Structure (London, England : 1993) Medium 39208792
2025 Cryo-EM structures of yeast Tom1 during an active ubiquitination cycle reveal that its extended solenoid domain architecture harbors a non-canonical ubiquitin-binding site that coordinates a structural ubiquitin; this structural ubiquitin contributes to the fidelity of K48-linked poly-ubiquitin chain assembly by the HECT domain. Cryo-electron microscopy (cryo-EM), in vitro ubiquitination assay, mutagenesis of ubiquitin-binding site Cell reports High 40359109
2025 The TOM1 G307D variant impairs TOM1-TOLLIP interaction and reduces TOM1's ability to inhibit TOLLIP binding to PI(3)P; patient cells show autophagosome accumulation due to impaired autophagosome-lysosome fusion and excessive activation of inflammatory pathways. Biophysical binding assays, Co-immunoprecipitation, patient cell autophagy/lysosome fusion assays, inflammatory signaling assays Disease models & mechanisms Medium 40936361
2026 TOM1 binds the NTF2L domain of the stress granule scaffold protein G3BP1 and promotes stress granule disassembly; TOM1 upregulation during chronic colitis drives SG disassembly, enabling nuclear translocation of FUBP1 and upregulation of c-Myc, thereby contributing to tumorigenesis. Co-immunoprecipitation, overexpression, nuclear fractionation, cell-based SG assembly/disassembly assays Redox biology Medium 42127552

Source papers

Stage 0 corpus · 49 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2012 Autophagy receptors link myosin VI to autophagosomes to mediate Tom1-dependent autophagosome maturation and fusion with the lysosome. Nature cell biology 240 23023224
2010 miR-126 is downregulated in cystic fibrosis airway epithelial cells and regulates TOM1 expression. Journal of immunology (Baltimore, Md. : 1950) 163 20083669
2004 Tollip and Tom1 form a complex and recruit ubiquitin-conjugated proteins onto early endosomes. The Journal of biological chemistry 129 15047686
2003 Tom1, a VHS domain-containing protein, interacts with tollip, ubiquitin, and clathrin. The Journal of biological chemistry 115 14563850
1987 Establishment and characterization of a cell line, TOM-1, derived from a patient with Philadelphia chromosome-positive acute lymphocytic leukemia. Blood 68 3103721
2000 Structure of the VHS domain of human Tom1 (target of myb 1): insights into interactions with proteins and membranes. Biochemistry 61 10985773
1997 tom-1, a novel v-Myb target gene expressed in AMV- and E26-transformed myelomonocytic cells. The EMBO journal 59 9135152
2015 TOM1 is a PI5P effector involved in the regulation of endosomal maturation. Journal of cell science 53 25588840
2006 Recruitment of clathrin onto endosomes by the Tom1-Tollip complex. Biochemical and biophysical research communications 50 16412388
2008 Dictyostelium Tom1 participates to an ancestral ESCRT-0 complex. Traffic (Copenhagen, Denmark) 48 19054384
2004 Tom1 (target of Myb 1) is a novel negative regulator of interleukin-1- and tumor necrosis factor-induced signaling pathways. Biological & pharmaceutical bulletin 37 15056867
2003 Endofin recruits TOM1 to endosomes. The Journal of biological chemistry 32 14613930
2005 Endofin recruits clathrin to early endosomes via TOM1. Journal of cell science 31 15657082
1999 Yeast tom1 mutant exhibits pleiotropic defects in nuclear division, maintenance of nuclear structure and nucleocytoplasmic transport at high temperatures. Gene 31 10395901
2008 Overexpression of a host factor TOM1 inhibits tomato mosaic virus propagation and suppression of RNA silencing. Virology 28 18440043
2019 Amyloid-beta impairs TOM1-mediated IL-1R1 signaling. Proceedings of the National Academy of Sciences of the United States of America 24 31570577
2018 TOM1 Regulates Neuronal Accumulation of Amyloid-β Oligomers by FcγRIIb2 Variant in Alzheimer's Disease. The Journal of neuroscience : the official journal of the Society for Neuroscience 24 30185465
2013 [miR-126 inhibits colon cancer proliferation and invasion through targeting IRS1, SLC7A5 and TOM1 gene]. Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 24 23981989
1995 A high dose of the STM1 gene suppresses the temperature sensitivity of the tom1 and htr1 mutants in Saccharomyces cerevisiae. Biochimica et biophysica acta 24 7548221
2021 Protein Trafficking or Cell Signaling: A Dilemma for the Adaptor Protein TOM1. Frontiers in cell and developmental biology 21 33718385
2012 The Hect domain E3 ligase Tom1 and the F-box protein Dia2 control Cdc6 degradation in G1 phase. The Journal of biological chemistry 20 23129771
1999 TOM1 genes map to human chromosome 22q13.1 and mouse chromosome 8C1 and encode proteins similar to the endosomal proteins HGS and STAM. Genomics 20 10329004
2023 SENP1 modulates chronic intermittent hypoxia-induced inflammation of microglia and neuronal injury by inhibiting TOM1 pathway. International immunopharmacology 18 37137262
2008 Gene-based SNP mapping of a psychotic bipolar affective disorder linkage region on 22q12.3: association with HMG2L1 and TOM1. American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics 18 17671966
2019 Dominant TOM1 mutation associated with combined immunodeficiency and autoimmune disease. NPJ genomic medicine 15 31263572
2012 Hect E3 ubiquitin ligase Tom1 controls Dia2 degradation during the cell cycle. Molecular biology of the cell 14 22933573
2001 Genes encoding ribosomal proteins Rps0A/B of Saccharomyces cerevisiae interact with TOM1 mutants defective in ribosome synthesis. Genetics 14 11238398
2000 Extragenic suppressors that rescue defects in the heat stress response of the budding yeast mutant tom1. Molecular & general genetics : MGG 14 10660055
2024 SIRT5 induces autophagy and alleviates myocardial infarction via desuccinylation of TOM1. BMC cardiovascular disorders 13 39210272
2007 A TOM1 homologue is required for multiplication of Tobacco mosaic virus in Nicotiana benthamiana. Journal of Zhejiang University. Science. B 13 17444600
2000 The yeast peptidyl proline isomerases FPR3 and FPR4, in high copy numbers, suppress defects resulting from the absence of the E3 ubiquitin ligase TOM1. Molecular & general genetics : MGG 13 10821187
1999 Myb and Ets transcription factors cooperate at the myb-inducible promoter of the tom-1 gene. Biochimica et biophysica acta 13 10524199
2023 Editing of TOM1 gene in tobacco using CRISPR/Cas9 confers resistance to Tobacco mosaic virus. Molecular biology reports 12 37119416
2017 The ribosome-bound quality control complex remains associated to aberrant peptides during their proteasomal targeting and interacts with Tom1 to limit protein aggregation. Molecular biology of the cell 12 28298488
2016 Immunolocalization of Tom1 in relation to protein degradation systems in Alzheimer's disease. Journal of the neurological sciences 9 27206884
2012 Inhibition of TMV multiplication by siRNA constructs against TOM1 and TOM3 genes of Capsicum annuum. Journal of virological methods 8 22814091
2019 Preferential phosphatidylinositol 5-phosphate binding contributes to a destabilization of the VHS domain structure of Tom1. Scientific reports 5 31350523
2025 Structural ubiquitin contributes to K48 linkage specificity of the HECT ligase Tom1. Cell reports 4 40359109
2018 The HECT-type ubiquitin ligase Tom1 contributes to the turnover of Spo12, a component of the FEAR network, in G2/M phase. FEBS letters 4 29683484
2016 Structure of the GAT domain of the endosomal adapter protein Tom1. Data in brief 4 26977434
2022 The Penicillium chrysogenum tom1 Gene a Major Target of Transcription Factor MAT1-1-1 Encodes a Nuclear Protein Involved in Sporulation. Frontiers in fungal biology 3 37746180
2016 Backbone 1H, 15N, and 13C resonance assignments of the Tom1 VHS domain. Biomolecular NMR assignments 3 27704363
2025 A TOM1 variant impairs interaction with TOLLIP, autophagosome-lysosome fusion and regulation of innate immunity. Disease models & mechanisms 2 40936361
2023 TOM1 family conservation within the plant kingdom for tobacco mosaic virus accumulation. Molecular plant pathology 2 37443447
2024 An internal linker and pH biosensing by phosphatidylinositol 5-phosphate regulate the function of the ESCRT-0 component TOM1. Structure (London, England : 1993) 1 39208792
2015 Time to Fold: Tom1 Uses New Tricks to Regulate Lipid Binding of Tollip. Structure (London, England : 1993) 1 26445490
2026 Stress granule dynamics orchestrate colitis-to-cancer transition via TOM1-mediated disassembly and driven oncogenesis in response to inflammatory oxidative stress. Redox biology 0 42127552
2026 TOM1 family proteins: from cargo sorting to immune dysregulation and cancer. Cell communication and signaling : CCS 0 42192436
2025 Herb pair of Astragali Radix-Descurainiae Semen attenuate heart failure through the myosin VI-Tom1 complex mediated autophagy. Frontiers in cardiovascular medicine 0 40687556

Missed literature

Know a paper Affinage missed for TOM1? Flag it for the maintainers and the community.

No submissions yet.