Affinage

Showing TBP — TFIID is a alias.

TBP

TATA-box-binding protein · UniProt P20226

Length
339 aa
Mass
37.7 kDa
Annotated
2026-06-10
100 papers in source corpus 52 papers cited in narrative 52 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

TBP is the core promoter recognition factor that nucleates RNA polymerase preinitiation complex assembly by engaging the TATA element through the DNA minor groove (PMID:1760847) and bending the duplex ~80–90° around the TATA box (PMID:1736286, PMID:7533216); a discrete 12-residue surface determines its sequence specificity (PMID:1739977). TBP partitions into multiple multisubunit complexes—TAF-containing TFIID forms and TAF-independent forms in vivo (PMID:1936986, PMID:10818000)—and is directed into polymerase-selective complexes through mutually exclusive TAF binding, where SL1 TAFs and TFIID TAFs (TAF1/TAF250, TAF11/TAF13) compete for the same TBP surfaces (PMID:7801123, PMID:29111974). Within TFIID, TBP occupies the linker between lobes, and cryo-EM has resolved how downstream-promoter engagement by TAF1/TAF2 positions DNA so TBP scans for and loads onto the TATA box, a transition driven by TFIIA and promoter DNA (PMID:14765106, PMID:27007846, PMID:30442764). TBP delivery is tightly regulated: TFIIA dissociates inhibitory TBP/TFIID dimers and stabilizes TBP–DNA binding while relieving TAF-mediated autoinhibition (PMID:9300055, PMID:9603936, PMID:10518227), MOT1 uses ATP hydrolysis and a DNA handle to strip TBP from DNA (PMID:11296235), and SAGA hands TBP off to the promoter through a steric, TFIIA-dependent mechanism rather than co-binding (PMID:16888622, PMID:31969704). Once loaded, TBP–DNA is recognized by TFIIB to build the start-site-defining complex (PMID:7675079, PMID:9012349), and TFIID—but not TBP alone—is required for RNA Pol II promoter-proximal pausing (PMID:32229306). Beyond initiation, TBP is a transcriptional and regulatory hub: it is bound and modulated by p53, MYC, Mediator (via Med8), Brd2, and TAF15 (PMID:8405994, PMID:31686052, PMID:16964259, PMID:17111193, PMID:36261009), its protein levels are set by Huwe1-mediated K48 ubiquitination opposed by USP10 (PMID:26393420), and it stably bookmarks active promoters on mitotic chromosomes—recruiting PP2A to inactivate condensin and sustaining Pol II occupancy for post-mitotic transcriptional reactivation (PMID:11809839, PMID:18931662, PMID:26257282, PMID:29939130). Polyglutamine-expanded TBP causes the neurodegenerative disorder SCA17, where mutant TBP loses interactions with partners such as XBP1s and MyoD to alter target gene transcription (PMID:24462098, PMID:26387956).

Mechanistic history

Synthesis pass · year-by-year structured walk · 31 steps
  1. 1991 High

    Established the physical basis of promoter recognition by showing TBP reads the TATA box through the DNA minor groove rather than the major groove, defining an unusual mode of sequence-specific DNA binding.

    Evidence Base-substitution (C/I replacement) chemistry combined with DNA-binding assays

    PMID:1760847

    Open questions at the time
    • Did not resolve the atomic contacts or the protein surface mediating recognition
    • Did not address regulation of binding in vivo
  2. 1992 High

    Defined TBP's mechanical action on DNA and the protein determinant of specificity, showing TBP bends the helix and that a 12-residue region sets sequence preference.

    Evidence Permuted-site gel retardation with recombinant TBP; altered-specificity mutagenesis with biochemical and in vivo transcription assays

    PMID:1736286 PMID:1739977

    Open questions at the time
    • Bend angle quantified more precisely only later by EM
    • Did not connect bending to downstream PIC assembly
  3. 1992 High

    Revealed that TBP is not a free factor but resides in distinct multisubunit complexes with different functional capacities, only the larger of which supports activated transcription.

    Evidence Biochemical fractionation of HeLa extracts, cofractionation, in vitro transcription; B-TFIID subunit composition and ATPase assay

    PMID:1387711 PMID:1936986

    Open questions at the time
    • Full TAF composition of D-TFIID not enumerated
    • Functional role of B-TFIID ATPase unresolved
  4. 1993 High

    Identified direct TAF and regulator contacts on TBP, establishing TFIID architecture and linking TBP to oncogenic/tumor-suppressor regulation via TAF250, p53, and p300.

    Evidence Recombinant binding and yeast interaction (TAF250); Co-IP and EMSA (p53, p300); reconstitution of TAF60/70 ternary complexes

    PMID:7680771 PMID:8262073 PMID:8405994 PMID:8502484

    Open questions at the time
    • p300–TBP interaction may be indirect (Co-IP only)
    • Stoichiometry and assembly order of TAF subcomplexes not yet defined
  5. 1994 High

    Explained how TBP is sorted among polymerase systems and how cofactors modulate its DNA engagement, showing SL1 TAFs bind TBP mutually exclusively with TFIID TAFs and that TFIIA cooperatively enhances TBP–TATA binding.

    Evidence Reconstituted subunit competition assays (SL1); recombinant TFIIA reconstitution with DNase footprinting and in vitro transcription

    PMID:7801123 PMID:7958898

    Open questions at the time
    • Kinetics of TFIIA stabilization quantified only later
    • Mechanism of dimer/oligomer interplay not yet addressed
  6. 1995 High

    Provided atomic and structural mechanism for promoter engagement, showing TFIIB recognizes the preformed TBP–DNA complex and that TBP forms DNA-bending dimers/oligomers at the TATA box.

    Evidence X-ray crystallography of TFIIB/TBP/TATA; electron microscopy and mass analysis of TBP particles

    PMID:7533216 PMID:7675079

    Open questions at the time
    • Functional role of TBP oligomers in vivo unresolved
    • Did not capture the full PIC
  7. 1995 High

    Connected TBP availability to genome integrity and to small-molecule control, showing TBP is sequestered by damaged DNA and that minor-groove ligands block TBP loading.

    Evidence Filter binding, in vitro transcription competition, structural analysis, microinjection rescue; EMSA with distamycin/tallimustine

    PMID:7784168 PMID:9405373

    Open questions at the time
    • Physiological extent of TBP sequestration by lesions unquantified
    • Drug specificity for TBP versus general minor-groove occupancy unclear
  8. 1997 High

    Quantified and dissected TFIIA's role and the activator dependence on the TBP–TFIIB interface, showing TFIIA stabilizes TBP–DNA ~10-fold and that most but not all activators route through TBP–TFIIB contacts.

    Evidence Filter-binding koff measurements and EMSA (TFIIA); altered-specificity TBP–TFIIB array in human cells

    PMID:9012349 PMID:9300055

    Open questions at the time
    • Sp1 glutamine-rich domain's alternative path not mapped
    • In vivo kinetics on chromatin not addressed
  9. 1998 High

    Defined a regulatory logic of autoinhibition within TFIID, showing TAF250 inhibits TBP–DNA binding and TFIIA relieves this by competing for TBP.

    Evidence EMSA, DNase I footprinting, in vitro transcription, ts13 cell line

    PMID:9603936

    Open questions at the time
    • Structural basis of TAF250 occlusion resolved only later
    • In vivo balance of inhibition versus relief not quantified
  10. 1999 High

    Resolved how inhibitory TBP/TFIID dimers are cleared and how TBP loading is controlled in cells, showing TFIIA dissociates dimers and that Mot1, TFIIB, and Srb4 gate TBP occupancy at promoters.

    Evidence Kinetic dimerization/DNA-binding biochemistry; in vivo crosslinking-IP with yeast genetic epistasis

    PMID:10376604 PMID:10518227

    Open questions at the time
    • Mechanism of Mot1 action not yet biochemically dissected
    • Generality across promoter classes incompletely sampled
  11. 1999 Medium

    Distinguished TBP from paralogous TBP-related factors, showing TRF2 binds TFIIA/TFIIB but not canonical TATA boxes and localizes to distinct loci.

    Evidence Protein interaction assays and Drosophila polytene chromosome staining

    PMID:10220372

    Open questions at the time
    • TRF2 promoter specificity not yet defined (single-lab, descriptive)
    • Functional target genes not identified at this stage
  12. 2000 High

    Established in vivo that TBP occupancy, not TAF occupancy, tracks transcription, and that TBP exists in both TAF-dependent and TAF-independent active forms; in parallel, TRF1 was shown to direct a dedicated promoter class.

    Evidence ChIP at yeast promoters with genome-wide expression; reconstituted TRF1-directed transcription with multiple in vivo validations

    PMID:10797011 PMID:10818000

    Open questions at the time
    • Identity of the TAF-independent TBP form not biochemically defined
    • Mechanism of TAF-free TBP recruitment unclear
  13. 2001 High

    Solved the mechanism of MOT1-driven TBP removal, showing ATP hydrolysis and translocation along an upstream DNA handle strips TBP without strand separation or unbending.

    Evidence In vitro ATPase/disruption assays with template and substrate variants

    PMID:11296235

    Open questions at the time
    • Structural snapshot of MOT1–TBP–DNA not obtained
    • In vivo redistribution consequences not directly measured here
  14. 2002 High

    Revealed a non-canonical role of TBP in mitotic gene memory, showing TBP–TAF complexes remain stably promoter-bound on condensed mitotic chromosomes.

    Evidence FRAP of GFP-TBP in live HeLa cells and chromatin fractionation

    PMID:11809839

    Open questions at the time
    • Functional consequence of mitotic retention not yet established
    • Mechanism enforcing stable binding unknown at this point
  15. 2003 High

    Extended TBP regulation to Pol III, showing p53 represses tRNA gene transcription by acting on TBP-containing TFIIIB assembly.

    Evidence In vitro transcription, overexpression rescue, ChIP, Co-IP

    PMID:12773395

    Open questions at the time
    • Structural basis of p53–TBP modulation not resolved
    • Whether the same axis operates at Pol I unaddressed here
  16. 2004 Medium

    Demonstrated that TBP–TATA complexes are dynamic and disruptable during initiation/reinitiation using engineered RNA aptamers that compete with DNA even in the presence of TFIIA/TFIIB; EM placed TBP in the TFIID linker domain.

    Evidence SELEX aptamer binding and in vitro transcription; EM immunomapping of yeast TFIID

    PMID:14765106 PMID:15103022

    Open questions at the time
    • Aptamer relevance to physiological regulators uncertain (single lab)
    • TFIID immunomap limited in resolution
  17. 2006 High

    Defined a handoff mechanism for TBP delivery by SAGA and additional regulatory contacts, showing SAGA (via Spt8/Ada1) competes with DNA for TBP and that Med8 and Brd2 provide further TBP-binding interfaces.

    Evidence Crosslinking and competition binding (SAGA); crystallography plus binding and genetics (Med8); Co-IP and reporter assays (Brd2)

    PMID:16888622 PMID:16964259 PMID:17111193

    Open questions at the time
    • Brd2–TBP interaction supported by single-lab Co-IP
    • Structural picture of SAGA–TBP completed only later
  18. 2008 High

    Provided the functional mechanism of mitotic bookmarking, showing TBP recruits PP2A to dephosphorylate and inactivate condensin at active promoters, preventing compaction and enabling reactivation in daughter cells.

    Evidence Co-IP, ChIP-on-chip, condensin inactivation assays

    PMID:18931662

    Open questions at the time
    • How TBP selects which promoters to bookmark not defined
    • Direct structural basis of TBP–PP2A–condensin axis unresolved
  19. 2010 High

    Captured how an activator licenses TBP for promoter binding, showing Rap1 and TFIIA cooperatively commit TFIID via a conformational change that exposes TBP and a locked DNA loop.

    Evidence Cryo-EM of TFIID–TFIIA–Rap1–DNA complexes

    PMID:20559389

    Open questions at the time
    • Generality of the Rap1 mechanism to other activators not established
    • Resolution limited details of TBP–DNA contacts
  20. 2013 High

    Resolved the conformational gating of human TFIID, showing a large lobe-A translocation driven by TFIIA and DNA that enables promoter recognition, with all core promoter motifs mapped.

    Evidence Single-particle cryo-EM, DNA labeling, DNase footprinting

    PMID:23332750

    Open questions at the time
    • Kinetics of the conformational transition not measured
    • How modifications/regulators bias states unaddressed
  21. 2014 Medium

    Sharpened the TBP/TRF division of labor and connected TBP misfolding to disease, showing TRF2 (not TBP) drives TCT/ribosomal protein gene transcription and that Hsc70 decline worsens mutant TBP in SCA17 by impairing TBP–XBP1s-driven MANF expression.

    Evidence Depletion/overexpression with in vitro transcription and ChIP-seq (TRF2); SCA17 knock-in mouse with Co-IP and rescue (Hsc70/MANF)

    PMID:24462098 PMID:24958592

    Open questions at the time
    • SCA17 chaperone-MANF axis from single-lab mouse model
    • Direct structural basis of mutant TBP–partner loss not defined
  22. 2015 High

    Linked TBP to chromosome architecture and to its own turnover, showing TBP recruits condensin via Cnd2 to highly transcribed genes and that Huwe1/USP10 set TBP protein levels to control differentiation; polyQ expansion impairs MyoD–TBP coupling in muscle.

    Evidence Co-IP, ChIP, interaction-disrupting mutants (Cnd2); in vitro ubiquitination, MS, cellular assays (Huwe1/USP10); knockin mice with Co-IP/ChIP (polyQ)

    PMID:26257282 PMID:26387956 PMID:26393420

    Open questions at the time
    • polyQ–MyoD effects from single-lab model (Medium)
    • Coordination between TBP turnover and condensin recruitment unexplored
  23. 2016 High

    Achieved sub-nanometre definition of the promoter-engaged TFIID, showing TAF1/TAF2 dominate downstream-promoter contacts and TFIIA bridges TBP–TATA to lobe B.

    Evidence Single-particle cryo-EM of human TFIID–TFIIA–DNA

    PMID:27007846

    Open questions at the time
    • Dynamic loading pathway not yet resolved as discrete states
    • Side-by-side comparison with non-TATA promoters limited
  24. 2017 High

    Defined a further layer of TBP autoinhibition within TFIID, showing TAF11/TAF13 occupy the DNA-binding surface of TBP and compete with both TATA-DNA and the TAF1 TAND1 domain.

    Evidence Crystal structure of TAF11/TAF13/TBP, XL-MS, competition assays, genetic complementation

    PMID:29111974

    Open questions at the time
    • How TAF11/13 release is triggered during loading unclear
    • Interplay with TFIIA-mediated relief not fully integrated
  25. 2018 High

    Defined the TBP-loading pathway and the functional importance of mitotic retention, showing TFIID scans for the TATA box through multiple states and that TBP residency on mitotic chromosomes is required for post-mitotic transcriptional reactivation.

    Evidence Cryo-EM of five states with XL-MS (loading); single-molecule imaging and degron with ChIP (mitotic retention)

    PMID:29939130 PMID:30442764

    Open questions at the time
    • Determinants of which promoters retain TBP through mitosis unresolved
    • Timing relative to PP2A–condensin axis not directly linked
  26. 2018 High

    Revealed a cytoplasmic, chaperone-gated TFIID assembly route, showing CCT hands nascent TAF5 to TAF6-TAF9 to form a submodule and partition TAF9 between TFIID and SAGA.

    Evidence Quantitative proteomics, structural and mutational analysis, MS of submodules

    PMID:30510221

    Open questions at the time
    • Where TBP enters this assembly hierarchy not pinpointed here
    • Regulation of submodule flux unknown
  27. 2019 High

    Connected TFIID recruitment to chromatin modifications and to oncogenic activators, showing serotonylated H3K4me3 (H3K4me3Q5ser) potentiates TFIID binding and that MYC directly contacts TBP to drive its transcriptional activity.

    Evidence Transglutaminase enzyme assay, genome-wide chromatin analysis, TFIID binding (serotonylation); crystal structure and MYC mutagenesis (MYC–TBP)

    PMID:30867594 PMID:31686052

    Open questions at the time
    • How serotonylation alters TBP positioning structurally not resolved
    • MYC–TBP contribution relative to other MYC cofactors unquantified
  28. 2020 High

    Completed the structural mechanism of SAGA-mediated TBP delivery and revealed TFIID's role beyond initiation, showing SAGA's histone-fold octamer represses spurious TBP–DNA binding and hands TBP off via TFIIA, and that TFIID (not TBP alone) is required for Pol II pausing.

    Evidence Cryo-EM of SAGA–TBP with delivery assays; reconstituted in vitro transcription, TFIID depletion, PRO-seq

    PMID:31969704 PMID:32229306

    Open questions at the time
    • Which TAF subunit mediates the pausing function not pinpointed
    • How SAGA and TFIID delivery routes are coordinated in cells unclear
  29. 2021 High

    Explained the nucleosome barrier to TBP, showing TBP can bind nucleosomal DNA at defined superhelical locations (TFIIA-dependent and -independent) but in configurations incompatible with PIC assembly.

    Evidence Cryo-EM of three TBP–nucleosome structures and biochemical binding assays

    PMID:34301908

    Open questions at the time
    • In vivo relevance of nucleosomal TBP binding not established
    • How chromatin remodelers convert these states to active PIC unknown
  30. 2022 Medium

    Identified a competitive regulatory exchange on TBP linked to cancer phenotypes, showing TIF1γ displaces and ubiquitylates TAF15 from a TAF15/TBP complex required for IL-6-driven EMT.

    Evidence Co-IP, competition binding, ubiquitylation and nuclear-export assays, cellular functional assays

    PMID:36261009

    Open questions at the time
    • Single-lab mechanism; structural basis of TAF15/TBP not resolved
    • Generality beyond lung adenocarcinoma context untested
  31. 2023 High

    Defined how the TBP-containing TFIID is built, showing co-translational, TAF1-scaffolded assembly of cytoplasmic submodules onto the nascent TAF1 polypeptide.

    Evidence RNA-immunoprecipitation, single-molecule imaging, proteomics, structure-function analyses

    PMID:37386215

    Open questions at the time
    • Timing of TBP incorporation into the co-translational pathway not pinpointed
    • How assembly errors are corrected or degraded unaddressed

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the many competing TBP interfaces—TAF autoinhibition, TFIIA relief, MOT1 removal, SAGA handoff, chromatin-mark sensing, and Huwe1/USP10 turnover—are integrated in real time at individual promoters to set transcriptional output remains unresolved.
  • No unified kinetic model linking TBP delivery, removal, and degradation at native loci
  • Determinants of promoter-specific TBP retention through mitosis undefined
  • Structural basis of disease-causing polyQ expansion's altered interactions not solved

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003677 DNA binding 5 GO:0140110 transcription regulator activity 5 GO:0140223 general transcription initiation factor activity 3 GO:0042393 histone binding 2
Localization
GO:0000228 nuclear chromosome 3 GO:0005634 nucleus 3 GO:0005654 nucleoplasm 3 GO:0005829 cytosol 2
Pathway
R-HSA-74160 Gene expression (Transcription) 5 R-HSA-1640170 Cell Cycle 4 R-HSA-392499 Metabolism of proteins 3 R-HSA-4839726 Chromatin organization 2
Partners
CND2MOT1MYCP53TAF1TAF13TFIIATFIIB
Complex memberships
SAGASL1TFIIDTFIIIB

Evidence

Reading pass · 52 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1991 TFIID/TBP binds primarily within the minor groove of the TATA box DNA helix, established by replacing thymines and adenines with cytosines and inosines to swap major-groove identity while preserving the minor groove surface. Novel base-substitution approach (C/I replacement) combined with DNA-binding assays Cell High 1760847
1992 TBP induces DNA bending around the TATA element upon binding, demonstrated with recombinant yeast and human TBP using a permuted binding site/gel retardation assay. Permuted binding site/gel retardation assay with recombinant TBP Proceedings of the National Academy of Sciences of the United States of America High 1736286
1992 A 12 amino acid region of TBP directly contacts the TATA element and determines DNA-binding specificity; specific substitutions in this region alter specificity from TATAAA to TGTAAA both in yeast and when the equivalent substitutions are introduced into human TBP. Genetic selection for altered-specificity mutants, biochemical DNA-binding assays, in vivo transcription assays Cell High 1739977
1992 TBP in HeLa extracts exists in two distinct multisubunit complexes (~300 kDa B-TFIID and >700 kDa D-TFIID), both containing the 38 kDa TBP; only the larger complex supports transcriptional activation by acidic or glutamine-rich activators. Biochemical fractionation, cofractionation studies, antisera-based detection, in vitro transcription Genes & development High 1936986
1992 B-TFIID is composed of two subunits: TBP and a 170 kDa TAF specific to B-TFIID (not present in D-TFIID); purified B-TFIID has (d)ATPase activity. Biochemical purification, subunit composition analysis, ATPase activity assay Proceedings of the National Academy of Sciences of the United States of America Medium 1387711
1993 Recombinant hTAFII250 binds directly to TBP both in vitro and in yeast and participates in the formation of the TFIID complex; the CCG1/hTAFII250 gene is linked to cell-cycle progression. Recombinant protein expression, in vitro binding, yeast interaction assays Nature High 7680771
1993 p53 binds directly to TBP and cooperates with TBP or partially purified TFIID to enhance binding to a DNA fragment containing both a p53-binding site and a TATA box; p53 strongly inhibits TBP (but not TFIID) binding to a TATA box lacking a p53 site. Co-IP, DNA-binding assays (EMSA), in vivo cotransfection transcription assays Genes & development High 8405994
1993 p300 co-immunoprecipitates with TBP and shares two additional common phosphoproteins (64 and 59 kDa) with TBP complexes, indicating p300 interacts with TBP (possibly through intermediary proteins). Co-immunoprecipitation, partial proteolytic digest comparison Oncogene Medium 8502484
1993 Drosophila TAFII60 and human TAFII70 bind weakly to TBP and strongly to TAFII250; in combination with TBP and TAFII250 they form a stable ternary complex; dTAFII60 and hTAFII70 also directly interact with TAFII40. Recombinant protein expression, pull-down binding assays The EMBO journal Medium 8262073
1994 SL1 TAFIs (TAFI110, 63, 48) can each bind individually and specifically to TBP, and form a stable TBP-TAF complex; binding of SL1 TAFIs to TBP is mutually exclusive with binding of TFIID subunits (TAFII250 or TAFII150), establishing a mechanism for directing TBP into polymerase-selective complexes. Reconstituted subunit interaction assays, complementary cDNA expression, biochemical binding competition Science High 7801123
1994 Drosophila holo-TFIIA (L/S complex) binds TBP with high affinity and cooperatively with TBP to TATA box DNA (extended DNase footprint); holo-TFIIA stimulates basal transcription and enhances activation by Sp1, VP16, and NTF-1. Reconstitution of recombinant TFIIA subunits, subunit interaction studies, DNase footprinting, in vitro transcription Genes & development High 7958898
1994 The TBP subunit of TFIID crosslinks specifically to the TATA element; a 150 kDa TAF crosslinks to downstream regions (+10 to +15 and +35 to +47); a >205 kDa TAF crosslinks preferentially to +35 to +42; TAF-DNA interactions downstream are disrupted at elevated salt while TBP-TATA interaction is maintained. Protein-DNA crosslinking of TFIID complex Nucleic acids research Medium 8139922
1995 Crystal structure of the TFIIB/TBP/TATA-element ternary complex at 2.7 Å resolution: core TFIIB resembles cyclin A and recognizes the preformed TBP-DNA complex through protein-protein and protein-DNA contacts; the TFIIB amino-terminal domain forms the downstream surface where it could fix the transcription start site. X-ray crystallography Nature High 7675079
1995 Distamycin A and tallimustine bind the DNA minor groove and prevent TBP (and TBP-TFIIA, TBP-TFIIA-TFIIB) binding to the TATA box; once formed, TBP-containing complexes are more resistant to these drugs; both inhibit basal in vitro transcription at similar concentrations. EMSA (gel shift), in vitro transcription Nucleic acids research Medium 7784168
1995 Electron microscopy of yeast TBP on HIV and adeno promoters shows TBP forms dimers (63 kDa) at the TATA box and higher-order oligomers; TBP dimers bend the DNA approximately 80–90° around the TATA box. Electron microscopy (three preparative methods), mass analysis of protein particles Journal of molecular biology Medium 7533216
1997 TBP/TFIID binds selectively to cisplatin- or UV-damaged DNA (structural similarity between damaged DNA and TATA box), sequestering TBP away from promoters; microinjection of additional TBP in living human fibroblasts alleviates UV-induced reduction in RNA synthesis. Filter binding, in vitro transcription competition, 3D structural analysis, microinjection into living cells The EMBO journal High 9405373
1997 TFIIA stabilizes TBP/TATA interactions approximately 10-fold (reduces koff); upstream DNA contacts by TFIIA further stabilize the TFIIA-TBP complex; in the absence of DNA, TFIIA dissociates from TBP rapidly (koff ~4.9×10⁻³ s⁻¹). Nitrocellulose filter binding assay (koff measurements), EMSA, nickel-agarose pull-down with His-tagged TFIIA Journal of molecular biology High 9300055
1997 TBP-TFIIB interaction is critical for transcription in vitro; an altered-specificity TATA-TBP-TFIIB array showed that many activators use the known TBP-TFIIB interaction to stimulate transcription in human cells, but a glutamine-rich Sp1 activation domain activates independently of this interaction. Rationally designed altered-specificity TBP-TFIIB interaction, in vivo transcription assays in human cells Science High 9012349
1998 TAFIIs (specifically TAFII250) inhibit TBP-DNA binding and TBP-TFIIA binding; TFIIA overcomes TAFII-mediated inhibition of TBP-DNA binding by competing with TAFII250 for access to TBP; limited proteolysis of TFIID relieves inhibition of TBP-DNA binding. EMSA, DNase I footprinting, in vitro transcription, temperature-sensitive cell line (ts13) The Journal of biological chemistry High 9603936
1999 TFIIA promotes dissociation of TBP dimers (which block DNA binding) and accelerates TBP binding kinetics; TFIID dimer dissociation is slow and rate-limiting in DNA binding, and TFIIA induces rapid dissociation of TFIID dimers to facilitate promoter loading. Biochemical dimerization assays, kinetic DNA-binding assays Molecular cell High 10518227
1999 TBP binding in vivo is regulated: Mot1 prevents TBP binding to inactive promoters, and activator-mediated stimulation of TBP binding requires TFIIB and Srb4; TBP binding generally correlates with transcriptional activity across endogenous yeast genes. DNA-crosslinking/immunoprecipitation (in vivo), mutant yeast strains (genetic epistasis) Nature High 10376604
1999 TBP-related factor TRF2 (TBPL1) binds TFIIA and TFIIB but does not bind canonical TATA boxes; TRF2 is associated with distinct chromosomal loci from TBP in Drosophila, suggesting different promoter specificity. Protein interaction assays, Drosophila polytene chromosome staining Proceedings of the National Academy of Sciences of the United States of America Medium 10220372
2000 In vivo, TBP exists in at least two transcriptionally active forms: a TAF-containing form (TFIID) and a TAF-independent form; promoter occupancy by TAFs does not universally correlate with transcriptional activity, unlike TBP, TFIIA, and TFIIB occupancy. Chromatin immunoprecipitation (ChIP) at yeast promoters, genome-wide expression analysis Science High 10818000
2000 TBP-related factor TRF1 preferentially binds and directs transcription from the tudor gene promoter in Drosophila, a TRF1-responsive promoter distinct from TBP-regulated promoters. Polytene chromosome staining, ChIP, mRNA analysis, cotransfection assays, in vitro transcription reconstitution, DNase I footprinting Science High 10797011
2001 MOT1 uses ATP hydrolysis to remove TBP from DNA; MOT1 requires a 17 bp double-stranded DNA 'handle' upstream of the TATA box; TBP-DNA disruption does not require DNA strand separation, bending, or helix twisting, suggesting translocation along the handle drives disruption. In vitro ATPase/disruption assays with template variants, mutant DNA substrates The EMBO journal High 11296235
2002 GFP-TBP stably associates with condensed mitotic chromosomes (no FRAP signal during mitosis); in interphase cells, GFP-TBP shows ~100-fold slower FRAP than TFIIB; endogenous TBP and TAFs cofractionate with mitotic chromatin, suggesting TBP-TAF complexes remain promoter-bound for multiple rounds of transcription. FRAP of GFP-TBP in live HeLa cells, chromatin fractionation Molecular biology of the cell High 11809839
2003 p53 represses RNA polymerase III transcription through direct interaction with TBP; overexpressing TBP reverses p53-mediated inhibition of tRNA gene transcription; p53 does not disrupt TBP-Brf1 interaction but prevents Brf1 complexes from associating with TFIIIC2 and RNA pol III; ChIP shows TFIIIB occupancy decreases upon p53 induction. In vitro transcription, overexpression rescue, chromatin immunoprecipitation (ChIP), co-immunoprecipitation The EMBO journal High 12773395
2004 RNA aptamers generated against yeast TBP bind TBP competitively with TATA-DNA and some actively disrupt preformed TBP-TATA complexes even in the context of TFIIB and TFIIA; aptamers inhibit transcription in crude extracts, revealing dynamic TBP interactions during initiation and reinitiation. SELEX, aptamer binding assays, in vitro transcription with crude extracts Proceedings of the National Academy of Sciences of the United States of America Medium 15103022
2004 Immunomapping of yeast TFIID by EM revealed TBP is located in the linker domain between lobes A and C of the trilobed TFIID structure, with the N-terminal 100 residues of TAF1 spanning over TBP. Electron microscopy, immunomapping with antibodies against TFIID subunits The EMBO journal Medium 14765106
2006 SAGA binds TBP directly via its Spt8 subunit (and Ada1); Spt8 and SAGA compete with DNA (rather than forming a triple complex) to bind TBP, supporting a handoff model where SAGA transfers TBP to the TATA box rather than binding together with TBP at the promoter. Chemical crosslinking to identify SAGA-TBP contacts, direct binding assays (competition with DNA and TBP dimer) The EMBO journal High 16888622
2006 The Med8 N-terminal domain of the Mediator head subcomplex Med8-Med18-Med20 binds TBP in vitro and is essential in vivo; the Med8/18/20 subcomplex contains a putative multipartite TBP-binding site in the Mediator head. X-ray crystallography of Med8C/18/20 submodule, in vitro TBP-binding assays, genetic complementation Nature structural & molecular biology High 16964259
2006 Brd2 is a TBP-associated protein; a 26 amino acid peptide in the first bromodomain of Brd2 mediates Brd2-TBP interaction; serum stimulation induces formation of a Brd2-E2F-1-TBP complex in vivo, with Brd2 recruiting TBP into the E2F-1 transcriptional complex. Co-immunoprecipitation, domain mapping, overexpression assays, luciferase reporter assays Molecular and cellular biochemistry Medium 17111193
2008 During mitosis, TBP recruits PP2A to active gene promoters; the TBP-PP2A complex dephosphorylates and inactivates condensin at these promoters to prevent chromatin compaction, thereby bookmarking previously active genes for re-activation in daughter cells. Co-immunoprecipitation, ChIP-on-chip, functional assays for condensin inactivation Nature cell biology High 18931662
2010 TFIIA and the transactivator Rap1 cooperate to commit TFIID for transcription initiation; cryo-EM structures reveal Rap1 and TFIIA bind TFIID simultaneously, TFIIA undergoes a Rap1-induced conformational change that increases exposure of TBP within TFIID; a large DNA loop forms between the activator site and proximal promoter, locked by a TFIIA-Rap1 protein bridge. Cryo-electron microscopy, structural reconstruction of TFIID-TFIIA-Rap1-DNA complexes Nature High 20559389
2013 Human TFIID exists in two predominant structural states differing by ~100 Å translocation of lobe A; TFIIA and promoter DNA together facilitate transition to a rearranged state that enables promoter recognition; TATA, Inr, MTE, and DPE promoter motifs were mapped within the TFIID-TFIIA-DNA structure. Single-particle electron microscopy (cryo-EM), DNA labeling, DNase footprinting Cell High 23332750
2014 TRF2 (TBPL1/TRF2), not TBP, is required for transcription of TCT-dependent ribosomal protein genes in Drosophila and humans; TBP depletion does not affect TCT-dependent transcription, while TRF2 depletion does; purified TRF2 activates TCT but not TATA promoters in vitro. Overexpression and depletion of TBP/TRF2, in vitro transcription with purified TRF2, ChIP-seq Genes & development High 24958592
2014 Age-related decline in Hsc70 chaperone activity leads to increased mutant TBP accumulation in SCA17 mice; mutant TBP shows decreased association with XBP1s, resulting in reduced transcription of MANF; expression of Hsc70 improves TBP-XBP1s interaction and MANF transcription. Inducible knock-in mouse model, co-immunoprecipitation, gene expression analysis, MANF overexpression rescue Neuron Medium 24462098
2015 TBP interacts with the Cnd2 kleisin subunit of condensin and recruits condensin onto RNA polymerase III-transcribed genes and highly transcribed Pol II genes in fission yeast; disruption of the Cnd2-TBP interaction causes defects in condensin localization, mitotic chromosome assembly, chromosome segregation, and cellular lethality. Co-immunoprecipitation, ChIP, genetic analysis with interaction-disrupting mutants Molecular cell High 26257282
2015 TBP protein levels are regulated by the ubiquitin-proteasome system; E3 ligase Huwe1 targets TBP for K48-linked ubiquitination and proteasomal degradation; deubiquitinase USP10 antagonizes Huwe1 and protects TBP from degradation; upregulation of Huwe1 during myogenesis induces TBP degradation and drives myotube differentiation. In vitro ubiquitination assay with biochemical fractionation, mass spectrometry identification of Huwe1, cell-based functional assays eLife High 26393420
2016 Cryo-EM structure of human TFIID with TFIIA and core promoter DNA at sub-nanometre resolution shows TAF1 and TAF2 mediate major interactions with the downstream promoter; TFIIA bridges the TBP-TATA complex with lobe B of TFIID; all core promoter elements are contacted by TFIID subunits. Single-particle cryo-electron microscopy, structural analysis Nature High 27007846
2017 TAF11 and TAF13 form a ternary complex with TBP via the TAF11/TAF13 histone-fold domains; TAF11/TAF13 competes with TATA-box DNA and with the TAF1 N-terminal domain (TAND1) for TBP binding; the highly conserved C-terminal TBP-interaction domain (CTID) of TAF13 is essential for cell growth; TAF11/TAF13 interacts with the DNA-binding surface of TBP. Crystal structure of TAF11/TAF13/TBP complex (2.4 Å), cross-linking mass spectrometry, biochemical competition assays, genetic complementation eLife High 29111974
2018 Cryo-EM of human TFIID with TFIIA and promoter DNA reveals five structural states; initial TFIID binding to the downstream promoter positions upstream DNA and facilitates scanning of TBP for a TATA box, followed by engagement of the promoter — defining a mechanism for TBP loading onto promoter DNA by TFIID. Cryo-electron microscopy, chemical cross-linking mass spectrometry, biochemical reconstitution Science High 30442764
2018 TBP remains stably bound to active gene promoters on mitotic chromosomes in mouse ES cells (average residence time of minutes vs. seconds for typical TFs, by single-molecule live imaging); acute TBP degradation reduces RNA Pol II association with mitotic chromosomes and impairs transcriptional reactivation after mitosis. Live-cell single-molecule imaging, drug-inducible degron system, ChIP eLife High 29939130
2018 Chaperonin CCT specifically associates with nascent TAF5 and hands it off to TAF6-TAF9, acting as a checkpoint for TFIID assembly; TAF5-TAF6-TAF9 form a cytoplasmic submodule with novel interactions essential for TFIID integrity; TAF9 allocation between TFIID and SAGA is regulated by these submodule interactions. Quantitative proteomics, structural analysis, mutational analysis, mass spectrometry of submodules Nature structural & molecular biology High 30510221
2019 H3K4me3Q5ser (combinatorial serotonylation and methylation on histone H3) potentiates TFIID binding to H3K4me3 nucleosomes; tissue transglutaminase 2 serotonylates H3 at Q5 on H3K4me3-marked nucleosomes; ectopic expression of a H3 mutant that cannot be serotonylated alters H3K4me3Q5ser-target gene expression and impairs differentiation. In vitro enzyme assay (transglutaminase), genome-wide chromatin analysis, TFIID binding assay, H3 mutant cell lines Nature High 30867594
2019 Two discrete regions of MYC (amino acids 98–111 and 115–124) interact with TBP; a crystal structure (2.4 Å) shows MYC aa 98–111 interacts with TBP in the presence of TAF1 TAND1; MYC aa 115–124 resembles a TBP anchor motif; MYC mutants that abrogate TBP interaction compromise MYC transcriptional activity. 2.4 Å crystal structure, biochemical binding assays, site-specific MYC mutagenesis with functional assays Nature structural & molecular biology High 31686052
2020 Cryo-EM structure of yeast SAGA with bound TBP reveals an octamer of histone-fold domains in the SAGA core that forms a peripheral site for TBP where steric hindrance represses spurious DNA binding; biochemical analysis shows TBP delivery from SAGA to promoter DNA requires TFIIA and correlates with TBP-DNA affinity, supporting a handoff mechanism. Cryo-electron microscopy (3.5 Å core resolution), biochemical TBP delivery assays Nature High 31969704
2020 TFIID is required for RNA Polymerase II promoter-proximal pausing; replacement of TFIID with TBP alone in a purified reconstituted in vitro transcription system abolishes pausing; acute depletion of TFIID subunits in human and Drosophila cells disrupts genome-wide RNAPII pausing. Reconstituted in vitro transcription with purified factors, drug-inducible TFIID subunit depletion, PRO-seq Molecular cell High 32229306
2021 TBP can bind to a nucleosome containing the Widom-601 sequence; TFIIA stabilizes TBP-nucleosome binding; cryo-EM structures show TBP binds at SHL -6 (TATA-like, TFIIA-independent) and SHL +2 (GC-rich, TFIIA-dependent, with detachment of upstream terminal DNA from histone octamer); TBP-nucleosome complexes are sterically incompatible with PIC assembly. Cryo-electron microscopy (three structures), biochemical binding assays Proceedings of the National Academy of Sciences of the United States of America High 34301908
2022 TIF1γ binds TBP in competition with TAF15, displacing TAF15 from the TAF15/TBP complex; TIF1γ also multi-mono-ubiquitylates TAF15 and drives its nuclear export; the TAF15/TBP complex is required for IL-6 transactivation-induced EMT in lung adenocarcinoma cells. Co-immunoprecipitation, competition binding assays, ubiquitylation assays, nuclear export assays, cell functional assays Cell reports Medium 36261009
2023 Human TFIID biogenesis occurs co-translationally; all protein heterodimerization steps occur during protein synthesis; TAF1 acts as a flexible scaffold that drives co-translational recruitment of TFIID submodules preassembled in the cytoplasm onto the nascent TAF1 polypeptide. RNA-immunoprecipitation (RIP), single-molecule imaging, proteomics, structure-function analyses Nature structural & molecular biology High 37386215
2015 Large polyQ repeats (105Q) in TBP preferentially cause muscle degeneration and reduce expression of muscle-specific genes by decreasing the association of MyoD with TBP and with DNA promoters; smaller polyQ repeats do not show this effect, demonstrating polyQ length-dependent differential protein interactions. Knockin mouse models, co-immunoprecipitation, ChIP, direct muscle expression of TBP with different polyQ lengths Cell reports Medium 26387956

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1996 Biochemistry and structural biology of transcription factor IID (TFIID). Annual review of biochemistry 628 8811195
1995 Crystal structure of a TFIIB-TBP-TATA-element ternary complex. Nature 483 7675079
2019 Histone serotonylation is a permissive modification that enhances TFIID binding to H3K4me3. Nature 369 30867594
2000 Redundant roles for the TFIID and SAGA complexes in global transcription. Nature 299 10864329
1993 Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation. Nature 228 7680771
1999 Enhancement of TBP binding by activators and general transcription factors. Nature 211 10376604
1994 TBP-TAF complexes: selectivity factors for eukaryotic transcription. Current opinion in cell biology 207 7917332
2016 Structure of promoter-bound TFIID and model of human pre-initiation complex assembly. Nature 194 27007846
1991 TFIID binds in the minor groove of the TATA box. Cell 194 1760847
2003 Clinical features and neuropathology of autosomal dominant spinocerebellar ataxia (SCA17). Annals of neurology 174 12953269
2000 TBP-associated factors (TAFIIs): multiple, selective transcriptional mediators in common complexes. Trends in biochemical sciences 161 10664584
2000 TAF-Containing and TAF-independent forms of transcriptionally active TBP in vivo. Science (New York, N.Y.) 152 10818000
1992 Yeast and human TFIID with altered DNA-binding specificity for TATA elements. Cell 149 1739977
1993 An ATP-dependent inhibitor of TBP binding to DNA. Genes & development 148 8491381
1992 Promoter melting and TFIID complexes on Drosophila genes in vivo. Genes & development 148 1427079
1999 TATA box-binding protein (TBP)-related factor 2 (TRF2), a third member of the TBP family. Proceedings of the National Academy of Sciences of the United States of America 146 10220372
1997 Cisplatin- and UV-damaged DNA lure the basal transcription factor TFIID/TBP. The EMBO journal 144 9405373
1991 The mammalian TFIID protein is present in two functionally distinct complexes. Genes & development 139 1936986
2018 Structure of human TFIID and mechanism of TBP loading onto promoter DNA. Science (New York, N.Y.) 138 30442764
1994 Reconstitution of transcription factor SL1: exclusive binding of TBP by SL1 or TFIID subunits. Science (New York, N.Y.) 134 7801123
1992 Transcription factor TFIID induces DNA bending upon binding to the TATA element. Proceedings of the National Academy of Sciences of the United States of America 134 1736286
1993 Cooperative DNA binding of p53 with TFIID (TBP): a possible mechanism for transcriptional activation. Genes & development 132 8405994
2002 TBP dynamics in living human cells: constitutive association of TBP with mitotic chromosomes. Molecular biology of the cell 126 11809839
1993 p300, and p300-associated proteins, are components of TATA-binding protein (TBP) complexes. Oncogene 126 8502484
2003 p53 represses RNA polymerase III transcription by targeting TBP and inhibiting promoter occupancy by TFIIIB. The EMBO journal 117 12773395
2002 Activator-specific recruitment of TFIID and regulation of ribosomal protein genes in yeast. Molecular cell 117 11983173
2000 Distinct roles for TBP and TBP-like factor in early embryonic gene transcription in Xenopus. Science (New York, N.Y.) 111 11125147
2013 Human TFIID binds to core promoter DNA in a reorganized structural state. Cell 102 23332750
1994 Drosophila TFIIA directs cooperative DNA binding with TBP and mediates transcriptional activation. Genes & development 100 7958898
2020 Structure of SAGA and mechanism of TBP deposition on gene promoters. Nature 95 31969704
2006 Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20. Nature structural & molecular biology 94 16964259
2018 A stable mode of bookmarking by TBP recruits RNA polymerase II to mitotic chromosomes. eLife 92 29939130
2014 Age-dependent decrease in chaperone activity impairs MANF expression, leading to Purkinje cell degeneration in inducible SCA17 mice. Neuron 92 24462098
2000 Promoter-selective properties of the TBP-related factor TRF1. Science (New York, N.Y.) 92 10797011
2002 TFIID and human mediator coactivator complexes assemble cooperatively on promoter DNA. Genes & development 87 12130544
2020 TXNIP/TBP-2: A Master Regulator for Glucose Homeostasis. Antioxidants (Basel, Switzerland) 84 32824669
2008 The TBP-PP2A mitotic complex bookmarks genes by preventing condensin action. Nature cell biology 83 18931662
1992 Composition of transcription factor B-TFIID. Proceedings of the National Academy of Sciences of the United States of America 77 1387711
2006 Yeast TFIID serves as a coactivator for Rap1p by direct protein-protein interaction. Molecular and cellular biology 73 17074814
1995 Distamycin A and tallimustine inhibit TBP binding and basal in vitro transcription. Nucleic acids research 73 7784168
2006 SAGA binds TBP via its Spt8 subunit in competition with DNA: implications for TBP recruitment. The EMBO journal 72 16888622
2008 Spinocerebellar ataxia 17 (SCA17) and Huntington's disease-like 4 (HDL4). Cerebellum (London, England) 71 18418687
1993 Cloning and expression of Drosophila TAFII60 and human TAFII70 reveal conserved interactions with other subunits of TFIID. The EMBO journal 71 8262073
2013 A central role for TFIID in the pluripotent transcription circuitry. Nature 70 23503660
2014 TRF2, but not TBP, mediates the transcription of ribosomal protein genes. Genes & development 69 24958592
2020 TFIID Enables RNA Polymerase II Promoter-Proximal Pausing. Molecular cell 68 32229306
2018 A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute Myeloid Leukemia. Cancer cell 65 29316427
2004 Mapping key functional sites within yeast TFIID. The EMBO journal 64 14765106
2006 Opposing roles for Set2 and yFACT in regulating TBP binding at promoters. The EMBO journal 59 16977311
2019 Recent insights into the structure of TFIID, its assembly, and its binding to core promoter. Current opinion in structural biology 58 31751889
2010 TFIIA and the transactivator Rap1 cooperate to commit TFIID for transcription initiation. Nature 58 20559389
2009 Structures of three distinct activator-TFIID complexes. Genes & development 57 19571180
2007 Spinocerebellar ataxia type 17 (SCA17): oculomotor phenotype and clinical characterization of 15 Italian patients. Journal of neurology 50 17934876
2019 Multiple direct interactions of TBP with the MYC oncoprotein. Nature structural & molecular biology 49 31686052
1998 Transcription factor IIA derepresses TATA-binding protein (TBP)-associated factor inhibition of TBP-DNA binding. The Journal of biological chemistry 49 9603936
2006 Brd2 is a TBP-associated protein and recruits TBP into E2F-1 transcriptional complex in response to serum stimulation. Molecular and cellular biochemistry 47 17111193
2021 Digenic inheritance of STUB1 variants and TBP polyglutamine expansions explains the incomplete penetrance of SCA17 and SCA48. Genetics in medicine : official journal of the American College of Medical Genetics 46 34906452
2019 Promoter Recognition: Putting TFIID on the Spot. Trends in cell biology 46 31300188
2010 Distinct regulatory mechanisms of eukaryotic transcriptional activation by SAGA and TFIID. Biochimica et biophysica acta 46 20800707
1997 Dynamic interplay of TFIIA, TBP and TATA DNA. Journal of molecular biology 46 9300055
2014 Targeting TBP-Associated Factors in Ovarian Cancer. Frontiers in oncology 45 24653979
2018 Chaperonin CCT checkpoint function in basal transcription factor TFIID assembly. Nature structural & molecular biology 43 30510221
1994 Protein/DNA crosslinking of a TFIID complex reveals novel interactions downstream of the transcription start. Nucleic acids research 43 8139922
2017 Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID. eLife 42 29111974
2015 LOXL2 Oxidizes Methylated TAF10 and Controls TFIID-Dependent Genes during Neural Progenitor Differentiation. Molecular cell 42 25959397
2003 Phenotypical variability of expanded alleles in the TATA-binding protein gene. Reduced penetrance in SCA17? Journal of neurology 42 12574945
1999 Isolation of mouse TFIID and functional characterization of TBP and TFIID in mediating estrogen receptor and chromatin transcription. The Journal of biological chemistry 40 10438527
1997 Selective use of TBP and TFIIB revealed by a TATA-TBP-TFIIB array with altered specificity. Science (New York, N.Y.) 39 9012349
2018 Piperine ameliorates SCA17 neuropathology by reducing ER stress. Molecular neurodegeneration 38 29378605
1993 Molecular cloning, expression, and characterization of the Drosophila 85-kilodalton TFIID subunit. Molecular and cellular biology 38 8247000
2022 Role of the TATA-box binding protein (TBP) and associated family members in transcription regulation. Gene 36 35597524
2015 Large Polyglutamine Repeats Cause Muscle Degeneration in SCA17 Mice. Cell reports 36 26387956
2005 Spinocerebellar ataxia type 17: report of a family with reduced penetrance of an unstable Gln49 TBP allele, haplotype analysis supporting a founder effect for unstable alleles and comparative analysis of SCA17 genotypes. BMC medical genetics 36 15989694
2003 Low pH enhances Sp1 DNA binding activity and interaction with TBP. Nucleic acids research 36 12888513
2015 Interaction between TBP and Condensin Drives the Organization and Faithful Segregation of Mitotic Chromosomes. Molecular cell 35 26257282
2010 Direct transactivator-transcription factor IID (TFIID) contacts drive yeast ribosomal protein gene transcription. The Journal of biological chemistry 35 20189987
1999 TFIIA regulates TBP and TFIID dimers. Molecular cell 35 10518227
2021 Structures and implications of TBP-nucleosome complexes. Proceedings of the National Academy of Sciences of the United States of America 34 34301908
2001 MOT1-catalyzed TBP-DNA disruption: uncoupling DNA conformational change and role of upstream DNA. The EMBO journal 34 11296235
2018 Molecular structure of promoter-bound yeast TFIID. Nature communications 33 30405110
2004 Probing TBP interactions in transcription initiation and reinitiation with RNA aptamers that act in distinct modes. Proceedings of the National Academy of Sciences of the United States of America 33 15103022
2019 Transcription initiation factor TBP: old friend new questions. Biochemical Society transactions 32 30710057
2004 Purification of active TFIID from Saccharomyces cerevisiae. Extensive promoter contacts and co-activator function. The Journal of biological chemistry 32 15448131
2018 Spinocerebellar Ataxia Type 17 (SCA17). Advances in experimental medicine and biology 31 29427105
1999 Isolation of cDNA, chromosome mapping, and expression of the human TBP-like protein. Biochemical and biophysical research communications 31 10082669
2023 Hierarchical TAF1-dependent co-translational assembly of the basal transcription factor TFIID. Nature structural & molecular biology 30 37386215
2012 Role of the CCAAT-binding protein NFY in SCA17 pathogenesis. PloS one 30 22530004
2020 Molecular determinants underlying functional innovations of TBP and their impact on transcription initiation. Nature communications 29 32404905
2019 Epigenetics and transcription regulation during eukaryotic diversification: the saga of TFIID. Genes & development 29 31123066
2015 A specific E3 ligase/deubiquitinase pair modulates TBP protein levels during muscle differentiation. eLife 29 26393420
2014 Deactivation of TBP contributes to SCA17 pathogenesis. Human molecular genetics 29 25104854
2007 TBP paralogs accommodate metazoan- and vertebrate-specific developmental gene regulation. The EMBO journal 29 17703192
1995 Visualization of TBP oligomers binding and bending the HIV-1 and adeno promoters. Journal of molecular biology 29 7533216
2016 Molecular mechanisms underlying Spinocerebellar Ataxia 17 (SCA17) pathogenesis. Rare diseases (Austin, Tex.) 28 28032013
2010 Sequential recruitment of SAGA and TFIID in a genomic response to DNA damage in Saccharomyces cerevisiae. Molecular and cellular biology 28 20956559
2020 SAGA and TFIID: Friends of TBP drifting apart. Biochimica et biophysica acta. Gene regulatory mechanisms 27 32673655
2001 RNA polymerase II and TBP occupy the repressed CYC1 promoter. Molecular microbiology 27 11401707
2022 TIF1γ inhibits lung adenocarcinoma EMT and metastasis by interacting with the TAF15/TBP complex. Cell reports 26 36261009
2009 SCA17 repeat expansion: mildly expanded CAG/CAA repeat alleles in neurological disorders and the functional implications. Clinica chimica acta; international journal of clinical chemistry 25 20004653
2003 Molecular investigation of TBP allele length: a SCA17 cellular model and population study. Neurobiology of disease 25 12758065

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