Affinage

TBCA

Tubulin-specific chaperone A · UniProt O75347

Round 2 corrected
Length
108 aa
Mass
12.9 kDa
Annotated
2026-04-28
98 papers in source corpus 13 papers cited in narrative 13 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

TBCA is a small molecular chaperone that captures quasi-native beta-tubulin polypeptides emerging from the cytosolic chaperonin CCT in a 1:1 stoichiometric complex, functioning as the earliest post-chaperonin factor in the beta-tubulin folding pathway and as a component of the multi-cofactor tubulin assembly machine (TBCA–TBCE, TBCC, ARL2) that drives de novo alpha/beta-tubulin heterodimer formation via GTP hydrolysis-coupled conformational switching (PMID:8706133, PMID:23973072). Beyond de novo folding, TBCA primarily receives beta-tubulin from TBCE/TBCB-mediated dissociation of pre-existing heterodimers, thereby participating in a recycling pathway that controls the critical concentration of free tubulin heterodimers and microtubule dynamics; colchicine specifically blocks this recycling by preventing TBCE access to dimers (PMID:33968934, PMID:17184771). Loss of TBCA in mammalian cells collapses the soluble tubulin pool, disorganizes the microtubule cytoskeleton, and causes G1 cell-cycle arrest and cell death, and in the zebra finch brain TBCA is required for development of sexually dimorphic neural circuitry independently of estradiol signaling (PMID:15963512, PMID:25702708).

Mechanistic history

Synthesis pass · year-by-year structured walk · 7 steps
  1. 1996 High

    The foundational question of how newly translated beta-tubulin reaches its native, heterodimer-competent state was answered by reconstituting a sequential cofactor cascade: TBCA was identified as the first post-chaperonin factor that captures and stabilizes CCT-generated beta-tubulin folding intermediates upstream of cofactors D, E, and C.

    Evidence In vitro reconstitution of the complete beta-tubulin folding pathway with sequential cofactor addition and nucleotide-dependency assays

    PMID:8706133

    Open questions at the time
    • Structural basis of the TBCA–beta-tubulin interaction was not resolved
    • In vivo relevance of the reconstituted pathway was not tested in mammalian cells
    • Whether TBCA also participates in tubulin recycling was not addressed
  2. 2005 High

    Whether TBCA is essential in living cells was unknown; siRNA-mediated silencing demonstrated that TBCA loss collapses the soluble tubulin pool, disrupts microtubule organization, and arrests cells in G1, establishing TBCA as indispensable for microtubule homeostasis and cell viability.

    Evidence siRNA knockdown in HeLa and MCF-7 cells with immunofluorescence, flow cytometry, and tubulin solubility assays

    PMID:15963512

    Open questions at the time
    • Mechanism linking TBCA loss to G1 arrest (versus a secondary tubulin depletion effect) was not dissected
    • No rescue experiment with exogenous TBCA was reported
  3. 2006 High

    It was unclear whether TBCA functions only in de novo folding or also in tubulin recycling; reconstitution of TBCE-mediated heterodimer dissociation showed that the released beta-tubulin is captured by TBCA in a 1:1 complex, establishing TBCA as a chaperone in the heterodimer recycling pathway.

    Evidence In vitro dissociation assays with purified native TBCE, non-denaturing gel electrophoresis, and Western blotting; complemented by overexpression studies identifying TBCE/TBCB binary complexes that enhance dissociation efficiency

    PMID:16624573 PMID:17184771

    Open questions at the time
    • Relative flux of beta-tubulin through de novo folding versus recycling was not quantified
    • Fate of TBCA-bound beta-tubulin after capture (re-assembly vs. degradation) was not resolved
  4. 2013 High

    The complete tubulin assembly machine was reconstituted, demonstrating how TBCA-bound beta-tubulin exchanges onto TBCD to form a supercomplex with TBCE/alpha-tubulin, and how TBCC triggers GTP hydrolysis at the beta-tubulin E-site to release native GDP-bound heterodimer—resolving the GTPase switch mechanism.

    Evidence Full reconstitution with recombinant cofactors in multiple host/vector systems, GTPase activity assays

    PMID:23973072

    Open questions at the time
    • No high-resolution structure of the TBCE/alpha/TBCD/beta supercomplex
    • Kinetic parameters of the exchange between TBCA and TBCD were not determined
  5. 2015 Medium

    The crystal structure of a TBCA ortholog revealed a conserved electrostatic surface likely mediating interaction with the beta-tubulin C-terminal tail, providing the first structural framework for understanding the TBCA–beta-tubulin interface.

    Evidence X-ray crystallography of Leishmania major TBCA with structural comparison across orthologs

    PMID:25945706

    Open questions at the time
    • No co-crystal structure with beta-tubulin was obtained
    • Functional validation of predicted interaction residues (mutagenesis) was not performed
    • Structure is from a parasite ortholog; human TBCA structure is lacking
  6. 2015 Medium

    Whether TBCA has tissue-specific developmental roles beyond generic tubulin homeostasis was open; in vivo knockdown in developing zebra finch brain showed that TBCA is required for growth and connectivity of a sexually dimorphic song nucleus, independently of estradiol signaling.

    Evidence Unilateral siRNA delivery in vivo, anterograde tract tracing, and morphometric analysis of brain nuclei in zebra finches

    PMID:25702708

    Open questions at the time
    • Whether the neural phenotype is a direct consequence of microtubule defects or involves other TBCA activities is unresolved
    • No mammalian neural phenotype has been reported
  7. 2021 High

    The mechanistic basis of colchicine's specificity was clarified: colchicine, but not nocodazole or cold shock, blocks TBCE/TBCB-mediated heterodimer dissociation, freeing TBCA from its beta-tubulin complex, and TBCA level manipulation showed that TBCA primarily receives beta-tubulin from recycling rather than de novo synthesis.

    Evidence In vitro dissociation assays, non-denaturing gel electrophoresis, and Western blotting of TBCA/beta-tubulin complexes in colchicine-treated human cells; RNAi and overexpression of TBCA with comparison to nocodazole, cold shock, and cycloheximide treatments

    PMID:33968934

    Open questions at the time
    • Structural mechanism by which colchicine prevents TBCE access to heterodimers is not resolved
    • Quantitative contribution of recycling versus de novo pathways to steady-state tubulin pools remains unspecified

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key open questions include the high-resolution structure of the human TBCA–beta-tubulin complex, the in vivo flux partitioning between de novo folding and recycling pathways, the molecular mechanism linking TBCA depletion to cell-cycle arrest, and whether TBCA has non-tubulin substrates or functions.
  • No human TBCA–beta-tubulin co-crystal or cryo-EM structure
  • Mechanism of G1 arrest upon TBCA depletion not molecularly resolved
  • Potential non-tubulin functions of TBCA are unexplored

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0044183 protein folding chaperone 6
Localization
GO:0005829 cytosol 5
Partners
TBCBTBCCTBCDTBCETUBB

Evidence

Reading pass · 13 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1996 TBCA (cofactor A) functions as an early post-chaperonin factor in the beta-tubulin folding pathway, capturing and stabilizing beta-tubulin folding intermediates generated by ATP-dependent interaction with the cytosolic chaperonin (CCT) in a quasi-native conformation. Cofactor A acts upstream of cofactors D, E, and C in a sequential cascade that does not require ATP or GTP hydrolysis (though GTP plays a structural role), ultimately yielding native beta-tubulin committed to the heterodimer state. In vitro reconstitution of folding pathway, sequential cofactor addition assays, GTP/ATP dependency experiments Cell High 8706133
2013 TBCA and TBCB bind to and stabilize newly synthesized quasi-native beta- and alpha-tubulin polypeptides, respectively, following CCT interaction. There is free exchange of beta-tubulin between TBCA and TBCD, and of alpha-tubulin between TBCB and TBCE, forming TBCD/beta and TBCE/alpha complexes that interact to form a supercomplex (TBCE/alpha/TBCD/beta). TBCC then triggers GTP hydrolysis by beta-tubulin E-site in the supercomplex, acting as a switch for disassembly and release of native GDP-bound heterodimer. Reconstitution assays, purification of recombinant TBCs in multiple host/vector systems, GTPase assays, in vitro assembly machine reconstitution Methods in cell biology High 23973072
2005 TBCA is essential for cell viability in mammalian cells. siRNA-mediated silencing of TBCA in HeLa and MCF-7 cells produces a decrease in the amount of soluble tubulin, modifications in microtubule organization, and G1 cell cycle arrest. In MCF-7 cells, TBCA knockdown causes cell death preceded by a change in cell shape resembling differentiation. siRNA knockdown in human cell lines, immunofluorescence microscopy of microtubules, flow cytometry for cell cycle analysis, tubulin solubility assays FEBS letters High 15963512
2006 TBCE and TBCB form a binary complex that greatly enhances the efficiency of TBCE to dissociate tubulin heterodimers in vivo and in vitro. After heterodimer dissociation, TBCE, TBCB, and alpha-tubulin form a ternary complex, while the free beta-tubulin subunit is captured by TBCA in a 1:1 stoichiometric TBCA/beta-tubulin complex. These complexes may escort alpha-tubulin towards degradation or recycling. Overexpression studies in cells, in vitro dissociation assays, non-denaturing gel electrophoresis, specific antibody detection of complexes Experimental cell research High 17184771
2006 Purified native TBCE dissociates tubulin heterodimers to produce free alpha-tubulin (as an unstable TBCE-alpha-tubulin complex) and free beta-tubulin. The beta-tubulin released from heterodimer dissociation is captured by TBCA in a 1:1 stoichiometry, demonstrated by non-denaturing gel electrophoresis and specific antibodies, confirming TBCA's role as a beta-tubulin chaperone in the recycling pathway. Baculovirus expression/purification of native TBCE, in vitro tubulin dissociation assay, non-denaturing gel electrophoresis, Western blotting with specific antibodies Protein expression and purification High 16624573
2008 The pachygyria-causing alpha-tubulin R264C mutation leads to a failure of CCT-generated alpha-tubulin folding intermediates to stably interact with TBCB, revealing that stable TBCB interaction is a required step in the alpha-tubulin folding pathway downstream of CCT. TBCA's partner chaperone TBCB is thus essential for forming productive folding intermediates before the assembly of heterodimers. In vitro chaperonin folding assays, pulldown of CCT-generated intermediates with recombinant TBCB, structural analysis of R264C mutation Molecular biology of the cell High 18199681
2010 In vitro CCT-driven folding reactions confirm that TBCA participates as a functional component of the tubulin heterodimer assembly machine. TBCD and TBCC cooperate to stimulate GTP hydrolysis by beta-tubulin at heterodimer concentrations far below the polymerization threshold, and TBCA captures beta-tubulin in these reactions. In vitro CCT-driven folding assays, GTPase activating protein assays, recombinant human vs. bovine TBCD comparison Cytoskeleton (Hoboken, N.J.) High 20740604
2012 TBCA expression in mouse testis is regulated post-transcriptionally by natural antisense transcripts (NATs) from a second Tbca gene locus (Tbca16). The sense and antisense Tbca16 transcripts regulate Tbca13 mRNA levels during spermatogenesis; RNAi depletion of Tbca16 transcripts leads to increased Tbca13 transcript levels in spermatocytes, establishing a non-coding RNA regulatory mechanism controlling TBCA protein levels during microtubule-dependent spermatogenesis. RT-PCR, tandem mass spectrometry (confirming absence of TBCA16 protein), RNAi depletion in spermatocyte cell lines, in situ hybridization PloS one Medium 22880023
2013 TBCA functions as a positive regulator of clear cell renal cell carcinoma (ccRCC) progression. siRNA-mediated silencing of TBCA inhibits proliferation, promotes apoptosis, reduces invasion and migration, disrupts cytoskeleton integration, affects cell size, and induces S/G2 cell cycle arrest with aberrant cyclin A/E and CDK2 expression in ccRCC cells. The mechanism involves TBCA's role in modulating cytoskeleton integrity and influencing cell cycle progression. siRNA knockdown and plasmid overexpression in ccRCC cell lines (786-O, Caki-1), cell proliferation assays, flow cytometry for apoptosis and cell cycle, invasion/migration assays, immunofluorescence of cytoskeleton International journal of cancer Medium 23740643
2015 The crystal structure of TBCA from Leishmania major was solved, revealing conserved features including a predicted electrostatic interaction surface likely involving the C-terminal tail of beta-tubulin. This structural analysis infers the mode of TBCA association with beta-tubulin during early stages of microtubule assembly. X-ray crystallography, structural comparison with three orthologous TBCA proteins Acta crystallographica. Section F, Structural biology communications Medium 25945706
2015 TBCA is involved in masculinization of the zebra finch song circuit. siRNA-mediated knockdown of TBCA in the lateral magnocellular nucleus of the anterior nidopallium (LMAN) reduced robust nucleus of the arcopallium (RA) cell number, cell size, and volume, and decreased the LMAN-to-RA axonal projection, demonstrating a direct role for TBCA in the development of a sexually dimorphic neural pathway. TBCA acts independently of estradiol signaling in this context. Unilateral siRNA delivery in vivo in developing zebra finches, anterograde tract tracing, morphometric analysis of brain nuclei, immunohistochemistry Journal of neuroendocrinology Medium 25702708
2021 Colchicine inhibits tubulin heterodimer dissociation by TBCE/TBCB, likely by interfering with TBCE interactions with tubulin dimers, leading to the release of free TBCA (uncomplexed to beta-tubulin). This is specific to colchicine and not observed with other anti-mitotic agents (nocodazole, cold shock) or translation inhibition (cycloheximide). Manipulation of TBCA levels by RNAi or overexpression results in decreased levels of tubulin heterodimers. The data strongly suggest that TBCA primarily receives beta-tubulin from dissociation of pre-existing heterodimers rather than from newly synthesized tubulins, and that the TBCE/TBCB+TBCA system controls the critical concentration of free tubulin heterodimers and microtubule dynamics by recycling heterodimers. In vitro tubulin dissociation assays, non-denaturing gel electrophoresis, Western blotting of TBCA/beta-tubulin complexes in colchicine-treated human cells, RNAi knockdown and overexpression of TBCA, comparison with nocodazole/cold shock/cycloheximide treatments Frontiers in cell and developmental biology High 33968934
2020 SARS-CoV-2 protein-protein interaction mapping by affinity-purification mass spectrometry identified TBCA as a high-confidence interaction partner of a SARS-CoV-2 viral protein in human cells, placing TBCA in the host interactome of this coronavirus. Affinity-purification mass spectrometry (AP-MS) of tagged SARS-CoV-2 proteins expressed in human cells Nature Low 32353859

Source papers

Stage 0 corpus · 98 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2020 A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature 3411 32353859
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2012 The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. Molecular cell 973 22681889
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2020 Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms. Science (New York, N.Y.) 564 33060197
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
2022 CST1 inhibits ferroptosis and promotes gastric cancer metastasis by regulating GPX4 protein stability via OTUB1. Oncogene 259 36369321
2022 Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration. Cell 256 35063084
1996 Pathway leading to correctly folded beta-tubulin. Cell 251 8706133
2017 Optimized fragmentation schemes and data analysis strategies for proteome-wide cross-link identification. Nature communications 221 28524877
2000 Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. Genome research 161 11042152
2013 Proteomic analysis of podocyte exosome-enriched fraction from normal human urine. Journal of proteomics 126 23376485
1998 Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning. Proceedings of the National Academy of Sciences of the United States of America 119 9653160
2007 Tetrabromocinnamic acid (TBCA) and related compounds represent a new class of specific protein kinase CK2 inhibitors. Chembiochem : a European journal of chemical biology 117 17133643
2007 Toward a confocal subcellular atlas of the human proteome. Molecular & cellular proteomics : MCP 114 18029348
2021 Protein interaction landscapes revealed by advanced in vivo cross-linking-mass spectrometry. Proceedings of the National Academy of Sciences of the United States of America 113 34349018
2010 Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Molecular medicine (Cambridge, Mass.) 108 20379614
1996 Chaperonin-mediated folding of actin and tubulin. The Journal of cell biology 106 8567715
2022 EZH2 depletion potentiates MYC degradation inhibiting neuroblastoma and small cell carcinoma tumor formation. Nature communications 99 35013218
2010 Genome-wide YFP fluorescence complementation screen identifies new regulators for telomere signaling in human cells. Molecular & cellular proteomics : MCP 93 21044950
2020 Kinase Interaction Network Expands Functional and Disease Roles of Human Kinases. Molecular cell 88 32707033
2014 Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation. Cell reports 80 24981860
2010 A genome-wide study reveals copy number variants exclusive to childhood obesity cases. American journal of human genetics 77 20950786
2019 The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis. Nature communications 74 31586073
2010 Human variation in alcohol response is influenced by variation in neuronal signaling genes. Alcoholism, clinical and experimental research 74 20201926
2015 Dimethyl fumarate confers neuroprotection by casein kinase 2 phosphorylation of Nrf2 in murine intracerebral hemorrhage. Neurobiology of disease 69 26176793
2020 Proteome-wide identification of HSP70/HSC70 chaperone clients in human cells. PLoS biology 65 32687490
2006 Role of cofactors B (TBCB) and E (TBCE) in tubulin heterodimer dissociation. Experimental cell research 63 17184771
2013 Tubulin-specific chaperones: components of a molecular machine that assembles the α/β heterodimer. Methods in cell biology 52 23973072
2013 Tubulin cofactor A functions as a novel positive regulator of ccRCC progression, invasion and metastasis. International journal of cancer 42 23740643
2010 Effect of TBCD and its regulatory interactor Arl2 on tubulin and microtubule integrity. Cytoskeleton (Hoboken, N.J.) 41 20740604
2013 Attenuation of β-amyloid-induced tauopathy via activation of CK2α/SIRT1: targeting for cilostazol. Journal of neuroscience research 40 24254769
2008 A pachygyria-causing alpha-tubulin mutation results in inefficient cycling with CCT and a deficient interaction with TBCB. Molecular biology of the cell 40 18199681
2005 Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death. FEBS letters 40 15963512
2017 A Trimer Consisting of the Tubulin-specific Chaperone D (TBCD), Regulatory GTPase ARL2, and β-Tubulin Is Required for Maintaining the Microtubule Network. The Journal of biological chemistry 39 28126905
2007 Transcriptome analysis of differentiating spermatogonia stimulated with kit ligand. Gene expression patterns : GEP 38 18036996
2017 Identification of potential pathogenic genes associated with osteoporosis. Bone & joint research 37 29203636
2011 Release of mitochondrial apoptogenic factors and cell death are mediated by CK2 and NADPH oxidase. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 35 22146192
2015 Acupuncture elicits neuroprotective effect by inhibiting NAPDH oxidase-mediated reactive oxygen species production in cerebral ischaemia. Scientific reports 33 26656460
2024 Serum proteomics reveal APOE-ε4-dependent and APOE-ε4-independent protein signatures in Alzheimer's disease. Nature aging 30 39169269
2019 Genome-wide DNA methylation analysis in ankylosing spondylitis identifies HLA-B*27 dependent and independent DNA methylation changes in whole blood. Journal of autoimmunity 30 31128893
2013 Globular adiponectin elicits neuroprotection by inhibiting NADPH oxidase-mediated oxidative damage in ischemic stroke. Neuroscience 30 23756185
2016 Hierridin B Isolated from a Marine Cyanobacterium Alters VDAC1, Mitochondrial Activity, and Cell Cycle Genes on HT-29 Colon Adenocarcinoma Cells. Marine drugs 29 27589771
2016 Infantile neurodegenerative disorder associated with mutations in TBCD, an essential gene in the tubulin heterodimer assembly pathway. Human molecular genetics 28 28158450
2014 Protein kinase CK2 inhibition induces cell death via early impact on mitochondrial function. Journal of cellular biochemistry 28 25043911
2018 Overexpression of adiponectin alleviates intracerebral hemorrhage-induced brain injury in rats via suppression of oxidative stress. Neuroscience letters 27 29870775
2010 Treatment of cultured human astrocytes and vascular endothelial cells with protein kinase CK2 inhibitors induces early changes in cell shape and cytoskeleton. Molecular and cellular biochemistry 27 21125314
2018 Proteomic alterations of fibroblasts induced by ovarian cancer cells reveal potential cancer targets. Neoplasma 26 28857608
2016 Comparative transcriptome analysis between an evolved abscisic acid-overproducing mutant Botrytis cinerea TBC-A and its ancestral strain Botrytis cinerea TBC-6. Scientific reports 25 27892476
2000 Differences in skeletal and muscle mass with aging in black and white women. American journal of physiology. Endocrinology and metabolism 25 10827019
2012 The expression of tubulin cofactor A (TBCA) is regulated by a noncoding antisense Tbca RNA during testis maturation. PloS one 22 22880023
2006 Cryptic out-of-frame translational initiation of TBCE rescues tubulin formation in compound heterozygous HRD. Proceedings of the National Academy of Sciences of the United States of America 21 16938882
2012 Casein kinase 2 inhibition attenuates androgen receptor function and cell proliferation in prostate cancer cells. The Prostate 20 22290244
2021 Colchicine Blocks Tubulin Heterodimer Recycling by Tubulin Cofactors TBCA, TBCB, and TBCE. Frontiers in cell and developmental biology 19 33968934
1996 Aging in women--the four-compartment model of body composition. Metabolism: clinical and experimental 19 8544776
2015 Abnormal Localization and Tumor Suppressor Function of Epithelial Tissue-Specific Transcription Factor ESE3 in Esophageal Squamous Cell Carcinoma. PloS one 18 25950810
2017 Nucleotide Binding to ARL2 in the TBCD∙ARL2∙β-Tubulin Complex Drives Conformational Changes in β-Tubulin. Journal of molecular biology 17 28970104
2006 Native tubulin-folding cofactor E purified from baculovirus-infected Sf9 cells dissociates tubulin dimers. Protein expression and purification 17 16624573
2015 The tubulin cofactor A is involved in hyphal growth, conidiation and cold sensitivity in Fusarium asiaticum. BMC microbiology 15 25886735
2012 Protection by cilostazol against amyloid-β(1-40)-induced suppression of viability and neurite elongation through activation of CK2α in HT22 mouse hippocampal cells. Journal of neuroscience research 15 22422579
2022 Polygenic Resilience Modulates the Penetrance of Parkinson Disease Genetic Risk Factors. Annals of neurology 14 35599344
2013 Evaluation of CK2 inhibitor (E)-3-(2,3,4,5-tetrabromophenyl)acrylic acid (TBCA) in regulation of platelet function. European journal of pharmacology 14 24140231
1993 In vivo animal models of body composition in aging. The Journal of nutrition 14 8429404
2021 Limitations in the comparison of the Brazilian National Dietary Surveys of 2008-2009 and 2017-2018. Revista de saude publica 13 34910053
2013 M9, a novel region of amino-Nogo-A, attenuates cerebral ischemic injury by inhibiting NADPH oxidase-derived superoxide production in mice. CNS neuroscience & therapeutics 12 23490284
2018 Cys2His2 Zinc Finger Transcription Factor BcabaR1 Positively Regulates Abscisic Acid Production in Botrytis cinerea. Applied and environmental microbiology 11 29959241
2013 A novel domain of amino-Nogo-A protects HT22 cells exposed to oxygen glucose deprivation by inhibiting NADPH oxidase activity. Cellular and molecular neurobiology 11 23354671
2022 Interactions between nuclear and mitochondrial SNPs and Parkinson's disease risk. Mitochondrion 9 35167983
2019 Multitarget-directed cotreatment with cilostazol and aripiprazole for augmented neuroprotection against oxidative stress-induced toxicity in HT22 mouse hippocampal cells. European journal of pharmacology 9 31202803
2017 Neuroprotection by aripiprazole against β-amyloid-induced toxicity by P-CK2α activation via inhibition of GSK-3β. Oncotarget 9 29299155
2022 Mutation-related magnetization-transfer, not axon density, drives white matter differences in premanifest Huntington disease: Evidence from in vivo ultra-strong gradient MRI. Human brain mapping 8 35396899
2012 Novel fluorescent cephalosporins: synthesis, antimicrobial activity and photodynamic inactivation of antibiotic resistant bacteria. European journal of medicinal chemistry 8 23220643
2009 Translation termination and protein folding pathway genes are not correlated in gastric cancer. Clinical chemistry and laboratory medicine 8 19284299
2016 Protein Kinase CK2 Regulates Leukocyte-Endothelial Cell Interactions during Ischemia and Reperfusion in Striated Skin Muscle. European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes 7 27287005
2022 Genome- and epigenome-wide studies of plasma protein biomarkers for Alzheimer's disease implicate TBCA and TREM2 in disease risk. Alzheimer's & dementia (Amsterdam, Netherlands) 6 35475137
2017 New insights on thyroid hormone mediated regulation of herpesvirus infections. Cell & bioscience 6 28344765
2022 Label-free quantitative proteomics and immunoblotting identifies immunoreactive and other excretory-secretory (E/S) proteins of Anoplocephala perfoliata. Frontiers in immunology 5 36466892
2015 Masculinisation of the zebra finch song system: roles of oestradiol and the Z-chromosome gene tubulin-specific chaperone protein A. Journal of neuroendocrinology 5 25702708
2011 Cationic recognition by tert-butylcalix[4]arene-functionalized nanoprobes. Physical chemistry chemical physics : PCCP 5 21327207
2020 Adiponectin alleviates non-alcoholic fatty liver injury via regulating oxidative stress in liver cells. Minerva medica 4 32996726
2013 Sexually dimorphic and developmentally regulated expression of tubulin-specific chaperone protein A in the LMAN of zebra finches. Neuroscience 4 23727504
2024 TBCA: Prediction of transcription factor binding sites using a deep neural network with lightweight attention mechanism. IEEE journal of biomedical and health informatics 3 38236675
2023 Serum proteomics reveals APOE dependent and independent protein signatures in Alzheimer's disease. medRxiv : the preprint server for health sciences 3 37986771
2015 The structure of tubulin-binding cofactor A from Leishmania major infers a mode of association during the early stages of microtubule assembly. Acta crystallographica. Section F, Structural biology communications 3 25945706
2025 Phenotypic variability in progressive encephalopathy with brain atrophy and thin corpus callosum: insights from two families. Neurogenetics 2 39853547
2025 Identifying space-resolved proteins of the murine thymus, by combining MALDI-MSI and proteomics. Life science alliance 1 41266110
2024 Serum proteomics reveals APOE dependent and independent protein signatures in Alzheimer's disease. Research square 1 38260284
2023 Control of aflatoxin biosynthesis by sulfur containing benzimidazole derivatives: In-silico interaction, biological activity, and gene regulation of Aspergillus flavus. Journal of biotechnology 1 37748651
2013 Scatter: a novel family of miniature inverted-repeat transposable elements in the fungus Botrytis cinerea. Journal of basic microbiology 1 23775675
2026 Senescence protein signatures predict dementia risk with causal implication for TBCA: a two-cohort study. Research square 0 41928789
2025 Identifying Space-Resolved Proteins of the Murine Thymus, by Combining MALDI Mass Spectrometry Imaging and Proteomics. bioRxiv : the preprint server for biology 0 40502118
2025 Proteomic signatures of the APOE ε4 and APOE ε2 genetic variants and Alzheimer's disease. medRxiv : the preprint server for health sciences 0 40799961
2025 Comparative proteome analysis of dried blood spots for high-risk group screening in children with autism spectrum disorder. Progress in neuro-psychopharmacology & biological psychiatry 0 40953633