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Showing SRA1 — SRA is a alias.

SRA1

Splicing factor, arginine/serine-rich 19 · UniProt Q9H7N4

Length
1312 aa
Mass
139.3 kDa
Annotated
2026-06-10
100 papers in source corpus 20 papers cited in narrative 20 extracted findings
Cross-family judge faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

SRA1 produces a functional non-coding RNA (SRA) that serves as a structured RNA scaffold and transcriptional coactivator, selectively enhancing steroid hormone receptor activity through their amino-terminal AF-1 domains (PMID:10199399, PMID:12943696). SRA acts as an RNA molecule rather than a protein: it functions under cycloheximide and tolerates translational stop-codon mutations, and discrete RNA secondary-structure motifs—not encoded amino acids—are required for coactivation (PMID:10199399, PMID:12444263). The active SRA RNA assembles into ribonucleoprotein coactivator complexes built around the SRC-1/TIF2 family, CBP/p300, and the DEAD-box helicases p68/p72, which bridge the receptor A/B domain to coactivators (PMID:11250900). Its activity is tuned by RNA-binding regulators that dock on defined substructures: SLIRP binds the STR7 element through an RRM and represses receptor transactivation by recruiting NCoR and modulating SRC-1 association (PMID:16762838), while pseudouridylation of U206 within STR5 hairpin by Pus1p is required for full steroid-receptor stimulation, and SRA binds the receptor N-terminal domain through a novel RNA-binding motif (PMID:22998747). Beyond canonical nuclear receptors, SRA/p68 act as broad transcriptional and architectural cofactors: they coactivate MyoD-driven myogenesis by assembling RNA Pol II and the Brg-1/SWI-SNF remodeler at muscle promoters (PMID:17011493), support PPARγ-driven adipogenesis (PMID:21152033), coactivate SF-1/Dax-1-dependent steroidogenesis (PMID:19188450), and stabilize cohesin at CTCF sites to maintain insulator function at the IGF2/H19 locus (PMID:20966046). SRA also associates with TrxG and PRC2 chromatin complexes and with NANOG to maintain pluripotency (PMID:26496121). In vivo, whole-body Sra1 knockout confers resistance to high-fat-diet obesity with improved insulin sensitivity, and SRA promotes hepatic steatosis by repressing ATGL via FoxO1 (PMID:24675075, PMID:27759039). The SRA1 locus additionally encodes a protein, SRAP, whose C-terminal domain adopts a five-helix bundle resembling the spliceosomal protein PRP18 rather than an RRM; its proposed direct binding to SRA RNA was not reproduced in structural and binding experiments (PMID:24486609).

Mechanistic history

Synthesis pass · year-by-year structured walk · 20 steps
  1. 1999 High

    Established the foundational and counterintuitive principle that SRA acts as an RNA, not a protein, defining a new class of non-coding RNA coactivator selective for steroid receptor AF-1 function.

    Evidence Transfection under cycloheximide, stop-codon mutagenesis, biochemical fractionation and Co-IP showing SRA in SRC-1-containing RNP complexes

    PMID:10199399

    Open questions at the time
    • Did not define the RNA structural determinants of activity
    • Did not resolve receptor-binding interface
  2. 2001 High

    Identified the protein composition of the SRA-associated ERα coactivator complex, placing SRA within an SRC-1/p300/p68-p72 assembly bridged to the receptor.

    Evidence Reciprocal Co-IP from MCF7 cells and HeLa extracts, immunofluorescence co-localization, reporter assays

    PMID:11250900

    Open questions at the time
    • Did not establish whether helicase binding is RNA-dependent
    • Stoichiometry of the complex unresolved
  3. 2002 High

    Provided definitive structural evidence that SRA acts through RNA motifs by showing silent mutations that preserve amino-acid sequence but disrupt RNA structure abolish coactivation.

    Evidence Comparative sequence/free-energy analysis, silent and deletion mutagenesis, reporter assays

    PMID:12444263

    Open questions at the time
    • Functional roles of individual motifs not fully assigned
    • No high-resolution RNA structure
  4. 2003 Medium

    Connected SRA coactivation to MAPK signaling by showing it requires ERα Ser118 and enables ligand-independent AF-1 activation.

    Evidence S118 mutagenesis with H-Ras(V12)-driven MAPK activation in reporter assays

    PMID:12943696

    Open questions at the time
    • Single lab
    • Mechanism linking phosphorylation to SRA recruitment unresolved
  5. 2004 Medium

    Refined receptor selectivity and phosphorylation-dependence, showing SRA enhances ERα (not ERβ) AF-1 independent of phosphorylation and modulates tamoxifen agonism.

    Evidence GAL4-AB fusion reporter assays, phospho-site mutagenesis, tamoxifen treatment

    PMID:15351741

    Open questions at the time
    • Apparent tension with S118-dependence not reconciled
    • Single lab
  6. 2006 High

    Resolved how SRA inhibitory regulation works mechanistically, identifying SLIRP as an RRM protein binding the STR7 substructure to repress receptor activity and recruit NCoR.

    Evidence Co-IP, ChIP, RRM mutagenesis, reporter assays and fractionation

    PMID:16762838

    Open questions at the time
    • How SLIRP and SRC-1 compete on SRA not fully resolved
    • In vivo relevance not tested
  7. 2006 High

    Extended SRA/p68-p72 beyond nuclear receptors to lineage-specifying transcription, showing they coactivate MyoD and are required for chromatin remodeling and Pol II/Brg-1 recruitment at muscle genes.

    Evidence Co-IP with MyoD, RNAi loss-of-function, chromatin remodeling assays and ChIP

    PMID:17011493

    Open questions at the time
    • Direct SRA-MyoD contact not defined
    • Role of SRA RNA structure here untested
  8. 2009 Medium

    Demonstrated SRA functions in steroidogenesis as a coactivator for SF-1 and Dax-1, linking it to control of steroidogenic gene expression.

    Evidence Co-IP, siRNA knockdown of endogenous SRA, reporter and RT-PCR readouts in Y1 cells

    PMID:19188450

    Open questions at the time
    • Single lab
    • Whether SF-1 binds SRA directly unresolved
  9. 2010 High

    Revealed an architectural, non-transcription-factor role: SRA/p68 stabilize cohesin at CTCF sites to enforce insulator function at the IGF2/H19 locus.

    Evidence Co-IP, ChIP showing cohesin loss without CTCF loss, chromosome conformation assays under siRNA depletion

    PMID:20966046

    Open questions at the time
    • Molecular basis of cohesin stabilization unresolved
    • Genome-wide generality not addressed
  10. 2010 Medium

    Linked SRA to adipogenesis and glucose metabolism by showing it associates with and coactivates PPARγ and promotes preadipocyte differentiation.

    Evidence Co-IP with PPARγ, gain/loss-of-function differentiation assays, microarray, glucose uptake assays

    PMID:21152033

    Open questions at the time
    • Single lab
    • Direct vs indirect PPARγ engagement unresolved
  11. 2010 Medium

    Defined the SRAP protein as an antagonist of SRA function in myogenesis, proposing SRAP binds the STR7 substructure via an RRM-like domain to inhibit MyoD coactivation.

    Evidence ncRNA-only vs SRAP-only constructs, RRM mutagenesis, Co-IP, myogenic differentiation assays

    PMID:20855289

    Open questions at the time
    • Proposed SRAP-SRA binding later contradicted (#15)
    • Single lab
  12. 2010 Medium

    Connected SRA to pluripotency gene regulation by showing Dax1 augments LRH-1-driven Oct4 activation in an SRA-dependent manner.

    Evidence Co-IP, ChIP at the Oct4 promoter, SRA knockdown in mES cells

    PMID:20943815

    Open questions at the time
    • Single lab
    • Direct SRA-Dax1/LRH-1 contacts not mapped
  13. 2012 Medium

    Identified a post-transcriptional RNA modification requirement, showing Pus1p-dependent pseudouridylation at U206 (STR5) is needed for steroid-receptor stimulation and that SRA binds the receptor NTD via a novel motif.

    Evidence In vitro pseudouridylation and RNA-protein binding assays, reporter assays, competitive inhibition with synthetic STR5

    PMID:22998747

    Open questions at the time
    • Single lab
    • In vivo requirement of pseudouridylation untested
  14. 2013 Medium

    Broadened SRA's coactivator partnerships to RISC machinery, showing Dicer/TRBP/PACT bind SRA and act as nuclear receptor coregulators at steroid-responsive promoters.

    Evidence Co-IP, ChIP, reporter assays, subcellular fractionation with Ago2 and pre-miRNA association

    PMID:23550157

    Open questions at the time
    • Single lab
    • Functional consequence of pre-miRNA co-association unclear
  15. 2013 Medium

    Extended SRA/Ddx5 coactivation to Notch signaling via the RBP-J transcription factor, linking SRA to p300 recruitment and histone acetylation at Notch targets.

    Evidence BioID/MS, Co-IP of Ddx5-RBP-J, ChIP, SRA knockdown/overexpression with RT-PCR

    PMID:23396200

    Open questions at the time
    • Single lab
    • Whether SRA contacts RBP-J directly unknown
  16. 2014 High

    Provided definitive in vivo metabolic function by showing Sra1 knockout mice resist diet-induced obesity with improved insulin sensitivity and reduced hepatic lipid.

    Evidence Whole-body knockout mouse, high-fat diet, metabolic phenotyping and gene expression analysis

    PMID:24675075

    Open questions at the time
    • Did not separate ncRNA from SRAP contributions in vivo
    • Tissue-specific mechanisms not dissected
  17. 2014 Medium

    Challenged the SRAP-as-RNA-binding-protein model by solving the SRAP C-terminal structure (PRP18-like five-helix bundle) and failing to detect specific SRAP-SRA RNA binding.

    Evidence Crystal structure, in vitro and cell-based RNA binding assays (negative), siRNA knockdown with RNA-seq

    PMID:24486609

    Open questions at the time
    • Direct contradiction with #9 unresolved
    • True molecular function of SRAP undefined
  18. 2015 Medium

    Placed SRA at the chromatin-state interface, showing it associates with both TrxG and PRC2, binds NANOG genome-wide, and is required for pluripotency and reprogramming.

    Evidence Co-IP, ChIP-seq, RIP-seq, loss-of-function in human pluripotent stem cells

    PMID:26496121

    Open questions at the time
    • Single lab
    • How SRA selects TrxG vs PRC2 outcomes unresolved
  19. 2016 Medium

    Defined a hepatic disease mechanism, showing SRA promotes steatosis by repressing ATGL through inhibition of FoxO1-driven ATGL induction.

    Evidence Knockout mice on high-fat diet, hepatocyte gain/loss-of-function, ATGL promoter-reporter assays

    PMID:27759039

    Open questions at the time
    • Single lab
    • Direct SRA-FoxO1 relationship not structurally defined
  20. 2021 Medium

    Revealed isoform-specific cancer relevance, showing SRSF1-driven exon 3 inclusion produces SRA1-L that promotes hepatocellular carcinoma invasion via CD44.

    Evidence RIP, CLIP, RNA pulldown, minigene-MS2 mutation, invasion and in vivo metastasis assays

    PMID:34011971

    Open questions at the time
    • Single lab
    • Whether the invasion effect is ncRNA- or SRAP-mediated unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • The molecular function of the SRAP protein and its relationship to the SRA ncRNA remain unresolved, as does how a single structured RNA is partitioned among its many coactivator and architectural complexes.
  • SRAP biochemical activity undefined and its proposed SRA-RNA binding contradicted
  • No high-resolution structure of active SRA RNP
  • Determinants directing SRA among receptor, chromatin, and insulator complexes unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 4 GO:0140110 transcription regulator activity 4 GO:0060090 molecular adaptor activity 3
Localization
GO:0005634 nucleus 3 GO:0005654 nucleoplasm 2
Pathway
R-HSA-1266738 Developmental Biology 3 R-HSA-1430728 Metabolism 3 R-HSA-162582 Signal Transduction 3 R-HSA-74160 Gene expression (Transcription) 3 R-HSA-4839726 Chromatin organization 2
Partners
CTCFDDX17DDX5NANOGPPARGRBPJSLIRPSRC-1
Complex memberships
CTCF-cohesin insulator complexPRC2SRC-1/p300 nuclear receptor coactivator complexTrxG chromatin complex

Evidence

Reading pass · 20 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1999 SRA functions as a non-coding RNA coactivator (not a translated protein) that selectively enhances steroid hormone receptor transactivation via their amino-terminal AF-1 activation function; it resides in distinct ribonucleoprotein complexes, one containing SRC-1. Evidence: SRA functions in presence of cycloheximide and retains activity with multiple translational stop codon mutations. Transient transfection with cycloheximide treatment, translational stop-codon mutagenesis, biochemical fractionation, co-immunoprecipitation Cell High 10199399
2002 Distinct RNA structural motifs within the SRA molecule are required for its coactivation function; six RNA motifs important for coactivation were identified by systematic mutagenesis using silent mutations that do not alter encoded amino acid sequence, providing strong evidence SRA acts as an RNA transcript rather than a protein. Comparative sequence analysis, free energy calculations, site-directed mutagenesis, genetic deletion analysis, transient transfection reporter assays Proceedings of the National Academy of Sciences of the United States of America High 12444263
2001 SRA associates with estrogen receptor alpha (ERα) coactivator complexes containing SRC-1/TIF2 family proteins, CBP/p300, and the RNA-binding DEAD-box proteins p72/p68; p72/p68 interact with both the AD2 domain of SRC-1/TIF2 and the ERα A/B domain, and all co-immunoprecipitate with estrogen-bound ERα in MCF7 cells. Co-immunoprecipitation from MCF7 cells and HeLa nuclear extracts, co-localization by immunofluorescence, transient transfection reporter assays The EMBO journal High 11250900
2003 SRA coactivation of ERα AF-1 requires an intact serine 118 (S118) residue; MAPK activation enables ligand-independent coactivation of ERα by SRA through the AF-1 domain in a mechanism independent of AF-2. SRA differentially activates ERα vs. ERβ through their respective AF-1 domains. Transient transfection reporter assays, site-directed mutagenesis of S118, H-Ras(V12) activation of MAPK pathway The Journal of steroid biochemistry and molecular biology Medium 12943696
2004 SLIRP (SRA stem-loop interacting RNA binding protein) binds the functional SRA substructure STR7 via an RNA recognition motif (RRM) and represses nuclear receptor transactivation in a SRA- and RRM-dependent manner; SLIRP modulates the association of SRC-1 with SRA, is recruited to endogenous promoters in a SRA-dependent manner, and recruits NCoR. Co-immunoprecipitation, chromatin immunoprecipitation, RRM mutagenesis, reporter assays, subcellular fractionation Molecular cell High 16762838
2006 RNA helicases p68/p72 and non-coding SRA RNA are coactivators of MyoD and required for skeletal muscle differentiation; p68/p72 promote assembly of TBP, RNA polymerase II, and the Brg-1 ATPase subunit of SWI/SNF at muscle gene promoters and facilitate chromatin remodeling. RNAi against p68/p72 or SRA prevents muscle gene activation and differentiation. Co-immunoprecipitation with MyoD, RNA interference, chromatin remodeling assays, ChIP, reporter assays Developmental cell High 17011493
2009 SRA RNA functions as a coactivator for steroidogenic factor 1 (SF-1) in steroidogenesis; both SF-1 and Dax-1 bind SRA, and coactivation by Dax-1 is abolished by SRA knockdown. Knockdown of endogenous SRA reduces expression of steroidogenic gene products StAR and melanocortin 2 receptor in adrenal Y1 cells. Co-immunoprecipitation, siRNA knockdown of endogenous SRA, reporter assays, RT-PCR for steroidogenic genes Molecular and cellular biology Medium 19188450
2010 SRA and RNA helicase p68 (DDX5) form a complex with CTCF that is essential for insulator function at the IGF2/H19 imprinted control region; SRA/p68 depletion reduces cohesin binding at CTCF sites without affecting CTCF binding itself, increases IGF2 expression, and increases enhancer-promoter interactions. The mechanism involves p68/SRA stabilizing interaction of cohesin with CTCF. Co-immunoprecipitation, ChIP, siRNA depletion, chromosome conformation assays Genes & development High 20966046
2010 SRA non-coding RNA associates with PPARγ and coactivates PPARγ-dependent transcription to promote adipocyte differentiation; SRA overexpression in ST2 mesenchymal precursor cells promotes adipogenesis, while SRA knockdown inhibits 3T3-L1 preadipocyte differentiation. SRA also increases insulin-stimulated glucose uptake, Akt and FOXO1 phosphorylation, and S-phase entry. Co-immunoprecipitation of SRA with PPARγ, reporter assays, SRA overexpression and siRNA knockdown, differentiation assays, microarray, glucose uptake assays PloS one Medium 21152033
2010 SRAP (the protein product of SRA1) prevents SRA ncRNA-dependent co-activation of MyoD during myogenic differentiation; the inhibitory effect is mediated through interaction of SRAP with SRA RNA via its RRM-like domain interacting with the SRA functional sub-structure STR7. Ratio of non-coding to coding SRA isoforms increases during normal myogenic differentiation but not in myotonic dystrophy patient cells. Constructs producing exclusively ncRNA or SRAP, reporter assays, RRM mutagenesis, co-immunoprecipitation, myogenic differentiation assays Nucleic acids research Medium 20855289
2013 RISC proteins PACT, TRBP, and Dicer are SRA-binding nuclear receptor coregulators; they target steroid-responsive promoters, regulate nuclear receptor activity, and associate with SRA and specific pre-microRNAs in both the nucleus and cytoplasm together with Argonaute 2. Co-immunoprecipitation, ChIP, nuclear receptor reporter assays, subcellular fractionation Proceedings of the National Academy of Sciences of the United States of America Medium 23550157
2013 RNA helicase Ddx5 and SRA function as coactivators of Notch signaling; Ddx5 directly interacts with the Notch transcription factor RBP-J and localizes to RBP-J binding sites at Notch target genes. SRA knockdown/overexpression reduces Notch target gene expression accompanied by loss of p300 occupancy and decreased histone acetylation. Biotinylation-tagging/mass spectrometry, Co-IP confirming Ddx5-RBP-J interaction, ChIP, Ddx5/SRA knockdown and overexpression with RT-PCR readout Biochimica et biophysica acta Medium 23396200
2014 SRA1 gene knockout in mice confers resistance to high fat diet-induced obesity, with decreased adipose mass, reduced adipocyte marker gene expression, improved insulin sensitivity (reduced fasting insulin, improved glucose tolerance), and reduced hepatic lipid droplets. SRA is expressed at highest levels in adipose tissue. Whole-body Sra1 gene knockout mouse model, high-fat diet feeding, metabolic phenotyping (glucose and insulin tolerance tests), gene expression analysis The Journal of biological chemistry High 24675075
2015 SRA associates with both trithorax group (TrxG) and polycomb repressive complex 2 (PRC2) complexes; p68 binding preferentially stabilizes SRA-TrxG but not SRA-PRC2 association. SRA binding sites co-occupied by p68 are enriched for H3K4me3 in human pluripotent stem cells. SRA directly interacts with NANOG and co-localizes with it genome-wide. SRA is required for maintaining pluripotency and reprogramming human fibroblasts. Co-immunoprecipitation of SRA with TrxG/PRC2, ChIP-seq, RIP-seq, loss-of-function experiments in pluripotent stem cells PLoS genetics Medium 26496121
2012 SRA requires pseudouridylation by Pus1p to stimulate steroid receptor signaling; uridine at position 206 (U206) in SRA hairpin STR5 is a critical pseudouridylation target. SRA binds directly to the N-terminal domain (NTD) of ERα and AR via a novel RNA-binding motif. A synthetic STR5 fragment competitively inhibits Pus1p-dependent pseudouridylation of SRA and inhibits ERα- and AR-dependent transactivation. In vitro pseudouridylation assay, RNA-protein binding assays with recombinant protein, transient transfection reporter assays, competitive inhibition with synthetic RNA Biochemistry Medium 22998747
2014 SRAP (SRA protein) crystal structure reveals a five-helix bundle carboxy-terminal domain similar to yeast spliceosome protein PRP18 rather than an RNA recognition motif; in vitro and cell-based binding experiments found no specific interaction between SRAP and SRA RNA, contradicting the previously proposed model. SRAP depletion had only small (~20%) effects on estrogen-responsive gene expression. Crystal structure determination of SRAP C-terminal domain, in vitro RNA binding assays with recombinant SRAP, plasmid-expressed SRAP and SRA RNA in cells, siRNA knockdown followed by RNA-seq Journal of molecular biology Medium 24486609
2004 SRA coactivation of ERα is phosphorylation-independent for the AF-1 A/B domain; SRA selectively enhances ERα but not ERβ AF-1 activity. SRA also enhances ERα AF-2 activity. SRA enhances 4-hydroxytamoxifen agonist activity on ERα but cannot convert tamoxifen to an ERβ agonist. Transient transfection reporter assays with GAL4-AB fusion constructs, phosphorylation site mutagenesis, tamoxifen treatment Biochemical and biophysical research communications Medium 15351741
2016 LncRNA SRA promotes hepatic steatosis by repressing expression of adipose triglyceride lipase (ATGL); SRA inhibits ATGL promoter activity primarily by inhibiting the inductive effects of transcription factor FoxO1. Loss of SRA in hepatocytes or SRA knockout mice induces ATGL expression and free fatty acid β-oxidation. SRA knockout mice on high-fat diet, SRA loss/gain-of-function in primary hepatocytes and hepatocyte cell line, ATGL promoter-reporter assays, RT-PCR and immunoblotting Scientific reports Medium 27759039
2021 SRSF1 promotes inclusion of exon 3 of SRA1 pre-mRNA by directly binding exon 3, increasing expression of the long SRA1-L isoform, which promotes hepatocellular carcinoma invasion through upregulation of CD44. SRA1-S (exon 3-skipped) opposes this effect. RNA immunoprecipitation, CLIP, RNA pulldown, and minigene-MS2 mutation experiments confirmed direct SRSF1-exon3 interaction. RNA immunoprecipitation (RIP), cross-link immunoprecipitation (CLIP), RNA pulldown, minigene-MS2 mutation assay, siRNA knockdown, invasion assays, in vivo lung metastasis model Cell death discovery Medium 34011971
2010 Dax1 augments LRH-1-mediated activation of the Oct4 gene in mouse embryonic stem cells in a SRA-dependent manner; Dax1 and LRH-1 co-immunoprecipitate in mES cells and co-localize at the Oct4 promoter. SRA knockdown abolishes Dax1 coactivation of Oct4. Co-immunoprecipitation, chromatin immunoprecipitation, luciferase reporter assays, SRA knockdown, overexpression/knockdown of Dax1 Molecular endocrinology (Baltimore, Md.) Medium 20943815

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2007 The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA. Nature 990 17994007
1999 A steroid receptor coactivator, SRA, functions as an RNA and is present in an SRC-1 complex. Cell 640 10199399
2008 Recognition of hemi-methylated DNA by the SRA protein UHRF1 by a base-flipping mechanism. Nature 397 18772891
2008 Structural basis for recognition of hemi-methylated DNA by the SRA domain of human UHRF1. Nature 381 18772889
2008 The SRA domain of UHRF1 flips 5-methylcytosine out of the DNA helix. Nature 344 18772888
2004 Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia formation. The EMBO journal 327 14765121
2007 The SRA methyl-cytosine-binding domain links DNA and histone methylation. Current biology : CB 316 17239600
2003 Abi, Sra1, and Kette control the stability and localization of SCAR/WAVE to regulate the formation of actin-based protrusions. Current biology : CB 294 14588242
2003 CYFIP/Sra-1 controls neuronal connectivity in Drosophila and links the Rac1 GTPase pathway to the fragile X protein. Neuron 266 12818175
2004 ICBP90, an E2F-1 target, recruits HDAC1 and binds to methyl-CpG through its SRA domain. Oncogene 265 15361834
2014 SRA- and SET-domain-containing proteins link RNA polymerase V occupancy to DNA methylation. Nature 260 24463519
2006 The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation. Developmental cell 249 17011493
2001 A subfamily of RNA-binding DEAD-box proteins acts as an estrogen receptor alpha coactivator through the N-terminal activation domain (AF-1) with an RNA coactivator, SRA. The EMBO journal 229 11250900
2008 Loss of SR-A and CD36 activity reduces atherosclerotic lesion complexity without abrogating foam cell formation in hyperlipidemic mice. Arteriosclerosis, thrombosis, and vascular biology 213 18948635
2005 CRMP-2 is involved in kinesin-1-dependent transport of the Sra-1/WAVE1 complex and axon formation. Molecular and cellular biology 207 16260607
2010 Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA. Genes & development 194 20966046
2010 Multiple roles for the non-coding RNA SRA in regulation of adipogenesis and insulin sensitivity. PloS one 176 21152033
2001 Identification of human-infective trypanosomes in animal reservoir of sleeping sickness in Uganda by means of serum-resistance-associated (SRA) gene. Lancet (London, England) 161 11755607
1993 Assignment of an autosomal sex reversal locus (SRA1) and campomelic dysplasia (CMPD1) to 17q24.3-q25.1. Nature genetics 159 8348155
2011 Recognition of 5-hydroxymethylcytosine by the Uhrf1 SRA domain. PloS one 145 21731699
2002 Distinct RNA motifs are important for coactivation of steroid hormone receptors by steroid receptor RNA activator (SRA). Proceedings of the National Academy of Sciences of the United States of America 131 12444263
2008 SRA-domain proteins required for DRM2-mediated de novo DNA methylation. PLoS genetics 129 19043555
2010 Steroid receptor RNA activator protein binds to and counteracts SRA RNA-mediated activation of MyoD and muscle differentiation. Nucleic acids research 117 20855289
1999 Analysis of macrophage scavenger receptor (SR-A) expression in human aortic atherosclerotic lesions. Arteriosclerosis, thrombosis, and vascular biology 117 10073945
2020 Formononetin attenuates atherosclerosis via regulating interaction between KLF4 and SRA in apoE-/- mice. Theranostics 116 31938053
2013 Hypoxia enhances lipid uptake in macrophages: role of the scavenger receptors Lox1, SRA, and CD36. Atherosclerosis 113 23706521
2012 The SRA protein UHRF1 promotes epigenetic crosstalks and is involved in prostate cancer progression. Oncogene 113 22330138
2006 SLIRP, a small SRA binding protein, is a nuclear receptor corepressor. Molecular cell 110 16762838
2005 Lecozotan (SRA-333): a selective serotonin 1A receptor antagonist that enhances the stimulated release of glutamate and acetylcholine in the hippocampus and possesses cognitive-enhancing properties. The Journal of pharmacology and experimental therapeutics 110 15951399
2010 Silencing of either SR-A or CD36 reduces atherosclerosis in hyperlipidaemic mice and reveals reciprocal upregulation of these receptors. Cardiovascular research 103 20634212
2014 SRA gene knockout protects against diet-induced obesity and improves glucose tolerance. The Journal of biological chemistry 101 24675075
2016 The lncRNA SLNCR1 Mediates Melanoma Invasion through a Conserved SRA1-like Region. Cell reports 100 27210747
2007 Steroid receptor RNA activator (SRA1): unusual bifaceted gene products with suspected relevance to breast cancer. Nuclear receptor signaling 100 17710122
2016 LncRNA SRA promotes hepatic steatosis through repressing the expression of adipose triglyceride lipase (ATGL). Scientific reports 96 27759039
2013 The DNA methyltransferase Dnmt1 directly interacts with the SET and RING finger-associated (SRA) domain of the multifunctional protein Uhrf1 to facilitate accession of the catalytic center to hemi-methylated DNA. The Journal of biological chemistry 94 24253042
2009 Dax-1 and steroid receptor RNA activator (SRA) function as transcriptional coactivators for steroidogenic factor 1 in steroidogenesis. Molecular and cellular biology 91 19188450
2014 Structural basis for hydroxymethylcytosine recognition by the SRA domain of UHRF2. Molecular cell 90 24813944
1994 The serum resistance-associated (SRA) gene of Trypanosoma brucei rhodesiense encodes a variant surface glycoprotein-like protein. Molecular and biochemical parasitology 90 7739673
2013 RNA-induced silencing complex (RISC) Proteins PACT, TRBP, and Dicer are SRA binding nuclear receptor coregulators. Proceedings of the National Academy of Sciences of the United States of America 87 23550157
2010 Protein complexes containing CYFIP/Sra/PIR121 coordinate Arf1 and Rac1 signalling during clathrin-AP-1-coated carrier biogenesis at the TGN. Nature cell biology 87 20228810
2006 Alternative splicing of the first intron of the steroid receptor RNA activator (SRA) participates in the generation of coding and noncoding RNA isoforms in breast cancer cell lines. DNA and cell biology 87 16848684
2012 Scavenger receptor A (SR-A) is required for LPS-induced TLR4 mediated NF-κB activation in macrophages. Biochimica et biophysica acta 81 22627090
1998 A naturally occurring isoform of the human macrophage scavenger receptor (SR-A) gene generated by alternative splicing blocks modified LDL uptake. Journal of lipid research 80 9548586
2008 Structure and hemimethylated CpG binding of the SRA domain from human UHRF1. The Journal of biological chemistry 79 18945682
2008 HBEGF, SRA1, and IK: Three cosegregating genes as determinants of cardiomyopathy. Genome research 79 19064678
2010 Clinical and immunological determinants of severe/refractory asthma (SRA): association with Staphylococcal superantigen-specific IgE antibodies. Allergy 78 20973803
2009 Increasing the relative expression of endogenous non-coding Steroid Receptor RNA Activator (SRA) in human breast cancer cells using modified oligonucleotides. Nucleic acids research 77 19483093
2008 The scavenger receptor SR-A I/II (CD204) signals via the receptor tyrosine kinase Mertk during apoptotic cell uptake by murine macrophages. Journal of leukocyte biology 76 18511575
2009 Pattern recognition scavenger receptor SRA/CD204 down-regulates Toll-like receptor 4 signaling-dependent CD8 T-cell activation. Blood 69 19349620
2010 EGb761 ameliorates the formation of foam cells by regulating the expression of SR-A and ABCA1: role of haem oxygenase-1. Cardiovascular research 67 20615914
2018 Expression Analysis of MALAT1, GAS5, SRA, and NEAT1 lncRNAs in Breast Cancer Tissues from Young Women and Women over 45 Years of Age. Molecular therapy. Nucleic acids 65 30126830
2012 PKCδ signalling regulates SR-A and CD36 expression and foam cell formation. Cardiovascular research 64 22687273
2001 Escherichia coli ribosome-associated protein SRA, whose copy number increases during stationary phase. Journal of bacteriology 64 11292794
2005 Efficacy and tolerability of darifenacin, a muscarinic M3 selective receptor antagonist (M3 SRA), compared with oxybutynin in the treatment of patients with overactive bladder. World journal of urology 62 16096831
2020 Xenbase: deep integration of GEO & SRA RNA-seq and ChIP-seq data in a model organism database. Nucleic acids research 59 31733057
2006 The calcineurin regulator sra plays an essential role in female meiosis in Drosophila. Current biology : CB 59 16860743
2010 Research resource: expression profiling reveals unexpected targets and functions of the human steroid receptor RNA activator (SRA) gene. Molecular endocrinology (Baltimore, Md.) 57 20219889
2019 Structure Relaxation Approximation (SRA) for Elucidation of Protein Structures from Ion Mobility Measurements. The journal of physical chemistry. B 55 30866623
2003 Ligand-independent coactivation of ERalpha AF-1 by steroid receptor RNA activator (SRA) via MAPK activation. The Journal of steroid biochemistry and molecular biology 53 12943696
2013 RNA helicase Ddx5 and the noncoding RNA SRA act as coactivators in the Notch signaling pathway. Biochimica et biophysica acta 52 23396200
2002 Synergically increased expression of CD36, CLA-1 and CD68, but not of SR-A and LOX-1, with the progression to foam cells from macrophages. Journal of atherosclerosis and thrombosis 52 12238639
1994 A point mutation leads to an unpaired cysteine residue and a molecular weight polymorphism of a functional platelet beta 3 integrin subunit. The Sra alloantigen system of GPIIIa. The Journal of biological chemistry 52 8132570
2017 Long non-coding RNA, steroid receptor RNA activator (SRA), induces tumor proliferation and invasion through the NOTCH pathway in cervical cancer cell lines. Oncology reports 50 29039612
2022 Dextran sulfate-based MMP-2 enzyme-sensitive SR-A receptor targeting nanomicelles for the treatment of rheumatoid arthritis. Drug delivery 49 35119317
2018 Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian Granular Cells of Mice. Medical science monitor : international medical journal of experimental and clinical research 49 29674607
2004 Sra-1 interacts with Kette and Wasp and is required for neuronal and bristle development in Drosophila. Development (Cambridge, England) 47 15269173
2011 Calcineurin and its regulator sra/DSCR1 are essential for sleep in Drosophila. The Journal of neuroscience : the official journal of the Society for Neuroscience 46 21900555
2003 Activation of signaling pathways by putative scavenger receptor class A (SR-A) ligands requires CD14 but not SR-A. Biochemical and biophysical research communications 46 14521944
2018 New Insights Into the Long Non-coding RNA SRA: Physiological Functions and Mechanisms of Action. Frontiers in medicine 44 30238005
2010 Dax1 up-regulates Oct4 expression in mouse embryonic stem cells via LRH-1 and SRA. Molecular endocrinology (Baltimore, Md.) 44 20943815
2011 Targeting the immunoregulator SRA/CD204 potentiates specific dendritic cell vaccine-induced T-cell response and antitumor immunity. Cancer research 43 21914786
2010 Calcineurin and its regulation by Sra/RCAN is required for completion of meiosis in Drosophila. Developmental biology 43 20561515
2016 Tandem virtual screening targeting the SRA domain of UHRF1 identifies a novel chemical tool modulating DNA methylation. European journal of medicinal chemistry 42 27049577
2013 SR-A and SREC-I are Kupffer and endothelial cell receptors for helper-dependent adenoviral vectors. Molecular therapy : the journal of the American Society of Gene Therapy 39 23358188
2015 Association of the Long Non-coding RNA Steroid Receptor RNA Activator (SRA) with TrxG and PRC2 Complexes. PLoS genetics 36 26496121
2007 The DEAD-box p68/p72 proteins and the noncoding RNA steroid receptor activator SRA: eclectic regulators of disparate biological functions. Cell cycle (Georgetown, Tex.) 36 17495528
2009 SRA and its binding partners: an expanding role for RNA-binding coregulators in nuclear receptor-mediated gene regulation. Critical reviews in biochemistry and molecular biology 35 19280430
2006 TLR2 and TLR4 agonists synergistically up-regulate SR-A in RAW264.7 through p38. Molecular immunology 35 17173973
2003 The C. elegans G-protein-coupled receptor SRA-13 inhibits RAS/MAPK signalling during olfaction and vulval development. Development (Cambridge, England) 35 12736202
2022 Using GenBank and SRA. Methods in molecular biology (Clifton, N.J.) 33 35037198
2016 Silencing of SRA1 Regulates ER Expression and Attenuates the Growth of Stromal Cells in Ovarian Endometriosis. Reproductive sciences (Thousand Oaks, Calif.) 33 27694140
2018 Targeting the SET and RING-associated (SRA) domain of ubiquitin-like, PHD and ring finger-containing 1 (UHRF1) for anti-cancer drug development. Oncotarget 32 29899856
2010 The scavenger receptors SRA-1 and SREC-I cooperate with TLR2 in the recognition of the hepatitis C virus non-structural protein 3 by dendritic cells. Journal of hepatology 32 20338659
2008 Human protein S inhibits the uptake of AcLDL and expression of SR-A through Mer receptor tyrosine kinase in human macrophages. Blood 32 18922854
2017 Roles of the Exogenous H2S-Mediated SR-A Signaling Pathway in Renal Ischemia/ Reperfusion Injury in Regulating Endoplasmic Reticulum Stress-Induced Autophagy in a Rat Model. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 31 28472786
2019 LncRNA-SRA1 Suppresses Osteosarcoma Cell Proliferation While Promoting Cell Apoptosis. Technology in cancer research & treatment 28 31106680
2016 Genetic variants in lncRNA SRA and risk of breast cancer. Oncotarget 28 26967566
2011 Recruitment of Dnmt1 roles of the SRA protein Np95 (Uhrf1) and other factors. Progress in molecular biology and translational science 28 21507355
2021 SRSF1 promotes the inclusion of exon 3 of SRA1 and the invasion of hepatocellular carcinoma cells by interacting with exon 3 of SRA1pre-mRNA. Cell death discovery 26 34011971
2012 SR-A deficiency reduces myocardial ischemia/reperfusion injury; involvement of increased microRNA-125b expression in macrophages. Biochimica et biophysica acta 26 23123599
2011 SR-A ligand and M-CSF dynamically regulate SR-A expression and function in primary macrophages via p38 MAPK activation. BMC immunology 26 21736734
2008 The RNA coregulator SRA, its binding proteins and nuclear receptor signaling activity. IUBMB life 26 18380007
2004 Human infectivity trait in Trypanosoma brucei: stability, heritability and relationship to sra expression. Parasitology 26 15521633
2011 Suppression of antigen-specific CD4+ T cell activation by SRA/CD204 through reducing the immunostimulatory capability of antigen-presenting cell. Journal of molecular medicine (Berlin, Germany) 25 22083206
2015 Recognition and cleavage of 5-methylcytosine DNA by bacterial SRA-HNH proteins. Nucleic acids research 24 25564526
2014 Structure and function of steroid receptor RNA activator protein, the proposed partner of SRA noncoding RNA. Journal of molecular biology 23 24486609
2011 Inflammatory stress exacerbates lipid-mediated renal injury in ApoE/CD36/SRA triple knockout mice. American journal of physiology. Renal physiology 23 21795641
2017 Decreased expression of LncRNA SRA1 in hepatocellular carcinoma and its clinical significance. Cancer biomarkers : section A of Disease markers 22 28085012
2004 SRA coactivation of estrogen receptor-alpha is phosphorylation-independent, and enhances 4-hydroxytamoxifen agonist activity. Biochemical and biophysical research communications 22 15351741
2012 A small RNA derived from RNA coactivator SRA blocks steroid receptor signaling via inhibition of Pus1p-mediated pseudouridylation of SRA: evidence of a novel RNA binding domain in the N-terminus of steroid receptors. Biochemistry 20 22998747

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