Affinage

SNW1

SNW domain-containing protein 1 · UniProt Q13573

Length
536 aa
Mass
61.5 kDa
Annotated
2026-04-28
100 papers in source corpus 29 papers cited in narrative 29 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

SNW1 (also called SKIP/NCoA-62) is a conserved nuclear protein that functions both as an essential spliceosome component and as a transcriptional coregulator linking multiple signaling pathways to RNA polymerase II elongation. Within the spliceosome, SNW1 resides in the U5 snRNP and activated (Bact) complex, directly contacts pre-mRNA branch-site nucleotides via its SNW domain, interacts with core splicing factors PRPF8, SNRNP200/Brr2, and EFTUD2/Snu114, and undergoes a disorder-to-order transition upon binding the cyclophilin PPIL1 through an N-terminal disordered region (PMID:26393790, PMID:16595688, PMID:25450007, PMID:25257309). Its spliceosomal activity is mechanistically required for sister chromatid cohesion, as SNW1 depletion causes intron retention in sororin and APC2 pre-mRNAs, and simultaneous expression of intron-less cDNAs rescues the cohesion defect (PMID:25257309). As a transcriptional coregulator, SNW1 bridges DNA-bound factors—including VDR, RAR, Notch/RBPJ, LEF1/β-catenin, and NF-κB—to P-TEFb (CycT1:CDK9), c-Myc, Menin, and TRRAP, while competing with corepressor complexes (SMRT/HDAC) for binding on the same transcription factors (PMID:9632709, PMID:10713164, PMID:15905409, PMID:19818711, PMID:30397075, PMID:20103590).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 1998 High

    The initial identification of SNW1/SKIP as a nuclear coactivator for the vitamin D receptor and as a Ski oncoprotein-interacting partner established its dual identity as a transcriptional coregulator connected to nuclear receptor signaling and oncogenic pathways.

    Evidence Yeast two-hybrid, GST pull-down, and transient transcription assays in mammalian cells; independent nuclear localization by immunofluorescence

    PMID:9569025 PMID:9632709

    Open questions at the time
    • Mechanism by which SKIP activates transcription (direct chromatin contact vs. bridging factor) was unknown
    • Whether SKIP had signaling-pathway-specific or general coactivator function was unresolved
  2. 2000 High

    Demonstration that SNW1 binds both NotchIC and the corepressor SMRT in a mutually exclusive manner on CBF1 revealed a competitive switching mechanism by which SNW1 converts CBF1 from a repressor to an activator during Notch signaling, and extended its coregulator role to Notch and EBNA2 viral transactivation.

    Evidence GST pull-down competition, antisense loss-of-function blocking Notch-dependent muscle differentiation inhibition, reporter assays; EBNA2 binding mapped to a distinct SKIP region

    PMID:10644367 PMID:10713164

    Open questions at the time
    • Whether SKIP displaces SMRT or is displaced by SMRT in vivo was not kinetically resolved
    • Whether SKIP's splicing and transcriptional coregulator roles are functionally separable remained unknown
  3. 2001 High

    Domain mapping established the SNW domain as the hub for transcriptional activation, VDR ternary complex formation with SRC coactivators, interactions with pRb, Ski, and HPV E7 oncoprotein, while the fission yeast ortholog linked SNW1 to the spliceosome via U2AF interaction and showed essentiality.

    Evidence Deletion/mutagenesis, ternary complex reconstitution, LXXLL peptide competition, yeast two-hybrid with U2AF23, gene deletion lethality in S. pombe

    PMID:11414703 PMID:11514567 PMID:11522815 PMID:11753645 PMID:12466551

    Open questions at the time
    • How SNW1 could simultaneously participate in splicing and transcription was mechanistically unclear
    • No structural information on the SNW domain itself was available
  4. 2004 Medium

    Cross-species complementation (human SNW1 rescuing yeast PRP45 deletion) proved SNW1 is a bona fide splicing factor, while ChIP showed ligand-dependent recruitment to VDR-responsive promoters at a late step after VDR/SRC entry, and its association with U5 snRNP components Prp8 and the 200-kDa helicase was demonstrated.

    Evidence Genetic complementation in S. cerevisiae, ChIP at endogenous promoters, co-IP with U5 components, nuclear matrix fractionation

    PMID:15194481 PMID:15225769

    Open questions at the time
    • Whether SNW1's splicing and transcriptional roles are exerted on the same gene simultaneously was unknown
    • No direct RNA contact by SNW1 had been demonstrated
  5. 2005 High

    The discovery that SNW1 recruits P-TEFb (CycT1:CDK9) to promote transcriptional elongation—demonstrated in the context of HIV-1 Tat transactivation—provided the first mechanistic explanation for how SNW1 activates transcription: by coupling elongation factor recruitment to DNA-bound activators and to alternative splice-site selection.

    Evidence Co-IP from nuclear extracts, in vitro transcription elongation assay showing stimulation of elongation but not initiation, RNAi, ChIP, alternative splice-site assay

    PMID:15905409

    Open questions at the time
    • Whether P-TEFb recruitment by SNW1 occurs in non-HIV transcriptional contexts was not yet shown
    • How SNW1 coordinates elongation factor delivery with its spliceosomal activity was unresolved
  6. 2006 High

    NMR structural determination of the PPIL1–SKIP complex revealed that a disordered N-terminal region of SKIP (residues 59–129) undergoes a disorder-to-order transition upon binding PPIL1, forming a hook-like structure; this interaction occurs at a site distinct from the PPIase active site, leaving PPIL1 catalytically competent within the activated spliceosome.

    Evidence NMR structure, SPR (Kd = 1.25 × 10⁻⁷ M), chemical shift perturbation, GST pull-down; confirmed by subsequent crystallography and peptide array

    PMID:16595688 PMID:20007319 PMID:20368803

    Open questions at the time
    • Functional consequence of PPIL1 isomerase activity while bound to SKIP was not identified
    • Whether the disorder-to-order transition is required for spliceosome catalytic activation was not tested
  7. 2009 High

    Genetic interaction mapping of yeast Prp45 with NTC components and second-step splicing factors established SNW1 as critical for the second catalytic step, especially for non-consensus splice sites, while in mammalian cells SNW1 was shown to recruit c-Myc, Menin, and TRRAP downstream of P-TEFb to chromatin.

    Evidence Synthetic lethality screen with NTC/step-2 factors, spliceosome purification, co-IP/RNAi-ChIP for c-Myc/Menin/TRRAP at HIV-1 promoter

    PMID:19016306 PMID:19818711

    Open questions at the time
    • Whether c-Myc/TRRAP recruitment is specific to viral promoters or general to SNW1-dependent genes was unknown
    • The structural basis for Prp45/SNW1 contacts at the catalytic center was not resolved
  8. 2010 Medium

    Identification of SNW1 as a component of a LEF1–HDAC1 ternary complex that mediates both transcriptional repression and Wnt-induced activation of target genes extended SNW1's coregulator function to the Wnt/β-catenin pathway, with C-terminal truncation stabilizing β-catenin.

    Evidence Co-IP, reporter assay, morpholino knockdown and overexpression in Xenopus embryos affecting neural crest induction

    PMID:20103590

    Open questions at the time
    • Whether SNW1 directly contacts β-catenin was not demonstrated
    • The relationship between SNW1's splicing role and its Wnt signaling role in neural crest was not dissected
  9. 2011 High

    In vivo epistasis experiments in Xenopus and zebrafish demonstrated that SNW1 regulates a specific domain of BMP signaling (phospho-Smad1) at the neural plate border required for neural crest specification, with BMP overexpression rescuing the SNW1 morphant phenotype.

    Evidence Morpholino knockdown in two organisms, pSmad1 immunostaining, BMP reporter transgenic, tissue explant assays, epistasis rescue

    PMID:21358802

    Open questions at the time
    • Whether SNW1 regulates BMP signaling through its splicing or transcriptional coregulator function was not resolved
    • No direct interaction between SNW1 and BMP pathway components was shown
  10. 2014 High

    Transcriptome-wide splicing analysis after SNW1 depletion revealed that its spliceosomal role is specifically required for sister chromatid cohesion through splicing of sororin and APC2 pre-mRNAs; simultaneous expression of intron-less cDNAs rescued cohesion, establishing a causal chain from splicing to mitotic fidelity.

    Evidence RNAi, RNA-seq, cohesin chromatin fractionation, intron-less cDNA rescue, live-cell imaging; domain-mapped interactions with EFTUD2 and SNRNP200

    PMID:25257309 PMID:25450007

    Open questions at the time
    • What feature of sororin/APC2 introns makes them particularly SNW1-dependent was not determined
    • Whether other mitotic phenotypes arise from SNW1-dependent splicing of additional transcripts was unexplored
  11. 2015 High

    UV cross-linking of purified Bact spliceosomes demonstrated that yeast Prp45 directly contacts nucleotides surrounding the branch site during catalytic activation, providing the first evidence that SNW1 physically engages the pre-mRNA substrate at the catalytic center.

    Evidence UV cross-linking with site-specifically labeled pre-mRNA in purified Bact complexes

    PMID:26393790

    Open questions at the time
    • Whether SNW1/Prp45 branch-site contact is catalytic or structural was not distinguished
    • No high-resolution structure of SNW1 within the intact spliceosome was available at this time
  12. 2019 High

    A genome-wide RNAi screen identified SNW1 as an NF-κB transcriptional coactivator that detaches from its spliceosomal complex upon NF-κB activation to bind the NF-κB heterodimer and recruit P-TEFb for elongation of inflammatory target genes, providing a signal-dependent mechanism for toggling between splicing and transcription functions.

    Evidence Genome-wide RNAi screen, co-IP showing dissociation from SNRNP200/SNRNP220, ChIP at IL-8/TNF promoters

    PMID:30397075

    Open questions at the time
    • The signal that triggers SNW1 release from the spliceosomal complex upon NF-κB activation was not identified
    • Whether this switching mechanism operates for other signaling pathways (Notch, VDR) was not tested

Open questions

Synthesis pass · forward-looking unresolved questions
  • A central unresolved question is how SNW1 partitions between its spliceosomal and transcriptional coregulator functions at the molecular level—whether these activities are temporally or spatially separated, and whether co-transcriptional splicing provides a unified context for both roles.
  • No high-resolution structure of human SNW1 in the spliceosomal context has been published in the timeline
  • Whether distinct SNW1 pools exist for splicing versus transcription has not been quantified
  • The full set of SNW1-dependent splice-sensitive transcripts beyond sororin/APC2 is not characterized

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 6 GO:0060090 molecular adaptor activity 5 GO:0003723 RNA binding 4 GO:0098772 molecular function regulator activity 4
Localization
GO:0005634 nucleus 4 GO:0005654 nucleoplasm 3
Pathway
R-HSA-8953854 Metabolism of RNA 8 R-HSA-74160 Gene expression (Transcription) 6 R-HSA-162582 Signal Transduction 4 R-HSA-1640170 Cell Cycle 1 R-HSA-168256 Immune System 1
Partners
CDK9EFTUD2PPIL1PRPF8RBPJSMRTSNRNP200VDR
Complex memberships
Activated spliceosome (Bact)P-TEFb (CycT1:CDK9)U5 snRNP

Evidence

Reading pass · 29 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 SNW1/SKIP (NCoA-62) was identified as a direct binding partner of the vitamin D receptor (VDR) ligand binding domain via yeast two-hybrid and in vitro GST pull-down assays, and its coexpression augmented 1,25-dihydroxyvitamin D3-activated transcription, establishing it as a transcriptional coactivator for VDR and other nuclear receptors. Yeast two-hybrid, GST pull-down, transient transcription assay The Journal of biological chemistry High 9632709
1998 SNW1/SKIP was identified as an interaction partner of the Ski oncoprotein via yeast two-hybrid and shown to be a nuclear protein homologous to Drosophila Bx42; the Ski-SKIP interaction maps to a conserved region of Ski required for transforming activity. Yeast two-hybrid, nuclear localization confirmed by immunofluorescence Oncogene Medium 9569025
2000 SNW1/SKIP was identified as a CBF1-binding protein that also interacts with NotchIC and the SMRT corepressor; the SMRT and NotchIC interactions with SKIP are mutually exclusive. A mutation in the fourth ankyrin repeat of Notch abolished interaction with SKIP but not CBF1, and antisense SKIP prevented NotchIC from blocking muscle differentiation, establishing SKIP as required for Notch signal transduction. Yeast two-hybrid, GST pull-down, competition binding, antisense loss-of-function, reporter assay Molecular and cellular biology High 10713164
2000 SNW1/SKIP interacts with the EBNA2 viral transactivator (via conserved region 5, distinct from the CBF1 interaction region) and with components of the CBF1 corepressor complex (SMRT, CIR, Sin3A, HDAC2); EBNA2 co-localizes with SKIP by immunofluorescence, and both CBF1 and SKIP contacts are required for efficient EBNA2-mediated promoter activation. Yeast two-hybrid, GST affinity assay, mammalian two-hybrid, immunofluorescence co-localization, reporter assay Journal of virology High 10644367
2001 The SNW domain of SKIP is the interaction region for Ski and is also the domain responsible for SKIP's transcriptional activation activity; addition of Ski further augments SNW-domain-dependent transcriptional activation. Deletion mapping, reporter transcription assay Nucleic acids research Medium 11522815
2001 SNW1/SKIP forms a ternary complex with the liganded VDR-RXR heterodimer and SRC coactivator proteins, contacts a distinct domain from SRC on VDR, and synergizes with SRCs to enhance VDR-mediated transcription; both coactivator classes are required. Ternary complex binding assay, LXXLL peptide competition, transactivation assay The Journal of biological chemistry High 11514567
2001 The fission yeast SKIP ortholog (spSNW1) interacts with the small subunit of the splicing factor U2AF (spU2AF23), linking SNW1 to the spliceosome; spSNW1 is an essential gene in S. pombe. Yeast two-hybrid, co-precipitation from nuclear extracts, gene deletion Biochemical and biophysical research communications Medium 11414703
2002 SNW1/SKIP interacts directly with the retinoblastoma protein (pRb) through amino acids 171–353 of the conserved SNW domain, and SKIP together with Ski can overcome pRb-induced transcriptional repression and G1 arrest. GST pull-down, reporter assay, cell-cycle phenotype rescue Nucleic acids research Medium 12466551
2002 The yeast SNW1 ortholog Prp45 SNW domain (N-terminal half, aa 1–190) is sufficient for viability; Prp45 activates reporter transcription in yeast dependent on conserved helical repeats and charged C-terminal domain; Prp45-GFP localizes to nuclear speckles. Complementation/deletion analysis, reporter transcription assay, GFP localization Journal of biochemistry Medium 12359070
2004 MAGE-A1 interacts with SKIP via yeast two-hybrid (confirmed biochemically), and through this interaction inhibits SKIP-dependent Notch1 transactivation; MAGE-A1 also recruits HDAC1 to act as a transcriptional repressor. Yeast two-hybrid, deletion analysis, reporter assay, HDAC recruitment assay Nucleic acids research Medium 15316101
2001 The HPV-16 E7 oncoprotein binds SKIP via its C-terminal region and the proline-rich sequences of the SNW domain, inhibiting SKIP transcriptional activation activity in vivo; transformation-defective E7 mutants fail both to bind SKIP and to inhibit its activity. Yeast two-hybrid, co-immunoprecipitation, reporter assay, E7 mutant analysis Oncogene Medium 11753645
2003 SNW1/SKIP interacts with specific residues in helix H10 of VDR (distinct from the AF-2/heterodimerization domain) in an AF-2-independent manner; these same H10 residues are also contacted by TFIIB and RXR, and mutation reduces SKIP interaction and ligand-dependent transactivation. GST pull-down, mutagenesis, transactivation assay The Journal of biological chemistry Medium 12529369
2004 SNW1/SKIP is a nuclear matrix-associated spliceosomal component and transcriptional coregulator whose SNW domain mediates interactions with splicing factors Prp8 and the U5 200 kDa helicase; ChIP shows ligand-dependent recruitment of NCoA-62/SKIP to VDR-responsive promoters at a distal step after VDR and SRC entry. Chromatin immunoprecipitation, nuclear fractionation, co-IP with splicing factors The Journal of steroid biochemistry and molecular biology Medium 15225769
2004 Human SKIP functionally complements deletion of the yeast PRP45 splicing gene, rescuing the lethal phenotype, demonstrating that SNW1/SKIP can act as a bona fide pre-mRNA splicing factor. Genetic complementation in S. cerevisiae Biochemical and biophysical research communications Medium 15194481
2005 SNW1/SKIP associates with P-TEFb (CycT1:CDK9) and Tat:P-TEFb complexes in nuclear extracts and with recombinant Tat:P-TEFb:TAR RNA in vitro; SKIP is required for Tat transactivation in vivo and stimulates HIV-1 transcription elongation but not initiation in vitro; SKIP also associates with U5snRNP proteins and facilitates recognition of an alternative Tat-specific splice site. All activities map to the SNW domain. Co-IP from nuclear extracts, in vitro transcription elongation assay, RNAi, ChIP, alternative splice-site assay Genes & development High 15905409
2006 PPIL1 (a cyclophilin spliceosomal component) directly binds a disordered N-terminal region of SKIP (residues 59–129) with a dissociation constant of 1.25×10⁻⁷ M; binding site on PPIL1 is distinct from the PPIase active site, leaving it available for isomerase activity; the complex is part of the 35S U5 snRNP and activated spliceosome. NMR structure determination, GST pull-down, surface plasmon resonance, chemical shift perturbation The Journal of biological chemistry High 16595688
2009 A large intrinsically disordered region in SKIP (residues 59–129) undergoes a disorder-to-order transition upon binding PPIL1; the minimal PPIL1-binding fragment (PBF, residues 59–79) forms a defined hook-like structure with PPIL1 via electrostatic and hydrophobic interactions; this structural rearrangement may facilitate spliceosome activation. NMR structure of PBF·PPIL1 complex, disordered-region characterization by NMR The Journal of biological chemistry High 20007319
2009 The yeast SNW1 ortholog Prp45 genetically interacts with NTC spliceosomal components (SYF1, CLF1, NTC20, CEF1) and second-step splicing factors (SLU7, PRP17, PRP18, PRP22); Prp45 affects the stoichiometry of Prp22 in Cwc2-associated spliceosomal complexes, and is required for efficient splicing of non-consensus substrates at the second step. Synthetic lethality screen, spliceosome purification/co-IP, in vivo splicing assays with mutant substrates Journal of cellular biochemistry High 19016306
2009 SNW1/SKIP associates with c-Myc and Menin (a subunit of the MLL1 H3K4me3 complex) and acts downstream of Tat:P-TEFb to recruit c-Myc and TRRAP to the HIV-1 promoter; SKIP is also recruited by the RNF20 H2B ubiquitin ligase to the basal HIV-1 promoter in a step bypassed by Tat. HIV-1 Tat transactivation requires c-Myc and Menin but not MLL1 or H3K4me3. Co-IP, RNAi-ChIP, reporter assay Molecular cell High 19818711
2009 SNW1/SKIP interacts with retinoic acid receptor (RAR) in a RA-dependent manner through a region overlapping the SIRT1 binding site; SKIP augments RAR transactivation by cooperating with SRC-1, while SIRT1 suppresses SKIP/SRC-1-enhanced RAR activity via its deacetylase activity; overexpression of SKIP impairs SIRT1 recruitment to the RARβ2 promoter. Binding assays, reporter assay, ChIP, RNAi, overexpression Nucleic acids research Medium 19934264
2010 Xenopus SKIP/SNW1 forms a ternary complex with LEF1 and HDAC1 and mediates transcriptional repression of Wnt target genes; SKIP is required for Wnt signaling-induced target gene transactivation; C-terminally truncated SKIP can stabilize β-catenin and enhance Wnt signaling; knockdown or overexpression of Skip in Xenopus reduces neural crest induction consistent with altered Wnt signaling. Co-IP, reporter assay, morpholino knockdown, overexpression in Xenopus embryos The Journal of biological chemistry Medium 20103590
2010 The crystal structure of PPIL1 bound to cyclosporine A was determined at 1.15 Å; a 36-residue SKIP fragment (centered on an 8-residue epitope) suffices to bind PPIL1 in pull-down experiments; molecular docking reveals a proline residue of SKIP buried in a hydrophobic pocket of PPIL1, surrounded by hydrogen bonds. X-ray crystallography (SAD phasing), peptide array, pull-down, molecular docking PloS one High 20368803
2011 SNW1 is required for neural crest specification in Xenopus and zebrafish; dorsally expressed SNW1 regulates a specific domain of BMP activity (phospho-Smad1) at the neural plate border at post-gastrula stages, acting upstream of BMP receptors; targeted BMP overexpression at the neural plate border restores neural crest formation in SNW1 morphants. Antisense morpholino knockdown in Xenopus and zebrafish, immunostaining for pSmad1, BMP reporter transgenic line, tissue explants, epistasis by BMP overexpression rescue PLoS biology High 21358802
2014 SNW1 and PRPF8 are essential for sister chromatid cohesion in human cells; transcriptome-wide analysis shows SNW1 depletion causes intron retention in a subset of pre-mRNAs including sororin (CDCA5) and APC2; reduced sororin levels destabilize cohesin on DNA, while reduced APC2 delays mitosis causing cohesion fatigue. Simultaneous expression of intron-less sororin and APC2 cDNAs restores cohesion in SNW1-depleted cells. RNAi depletion, RNA-seq transcriptome-wide splicing analysis, cohesin chromatin fractionation, cDNA rescue experiment, live-cell imaging The EMBO journal High 25257309
2014 SNW1 directly associates with spliceosome components EFTUD2 (Snu114) and SNRNP200 (Brr2) as shown by proteomics and biochemical assays; the SKIP region of SNW1 interacts with the N-terminus of EFTUD2 and two independent C-terminal regions of SNRNP200; depletion of SNW1 or EFTUD2 induces apoptosis in breast cancer cells. Proteomics, co-IP, domain mapping, RNAi, apoptosis assay Cancer medicine Medium 25450007
2015 UV cross-linking of purified yeast Bact spliceosomes shows that Prp45 (SNW1 ortholog) contacts nucleotides surrounding the branch-site upon step 1 catalysis; this interaction is enhanced relative to earlier spliceosomal complexes, indicating dynamic Prp45-pre-mRNA contacts during catalytic activation. UV cross-linking of purified spliceosomal complexes, site-specifically labeled pre-mRNA PLoS genetics High 26393790
2019 SNW1 detaches from its splicing complex (formed with SNRNP200 and SNRNP220) upon NF-κB activation, binds to the NF-κB heterodimer in the nucleus, and recruits p-TEFb to facilitate transcriptional elongation of NF-κB target genes (IL-8, TNF); SNW1 does not regulate cytoplasmic NF-κB pathway components. Genome-wide RNAi screen, co-IP, ChIP, RNAi knockdown with target gene expression readout Molecular and cellular biology High 30397075
2019 SNW1 acts as a novel RBPJ-interacting partner and regulates expression of Notch target genes in neuroblastoma; silencing RBPJ prevents SNW1-mediated Notch gene expression, placing SNW1 in the RBPJ-dependent Notch transcriptional complex. Co-IP, RNAi, reporter assay, gene expression analysis Biochemical and biophysical research communications Medium 30642633
2002 Drosophila Bx42 (SNW1 ortholog) is essential for development of many tissues; RNAi-mediated knockdown of Bx42 produces phenotypes resembling Notch mutants, and target genes of Notch signaling (cut, E(spl)m8) are suppressed; the wing phenotype from Suppressor of Hairless overexpression is strongly enhanced by simultaneous Bx42 RNAi, placing Bx42/SNW1 in the Notch pathway downstream of Notch signaling. Inducible RNAi in Drosophila, genetic epistasis with Notch pathway components, in situ target gene expression Mechanisms of development Medium 12204255

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1997 Frequency and therapeutic implications of "skip metastases" in the neck from squamous carcinoma of the oral tongue. Head & neck 295 9030939
2011 Arl8 and SKIP act together to link lysosomes to kinesin-1. Developmental cell 268 22172677
2000 SKIP, a CBF1-associated protein, interacts with the ankyrin repeat domain of NotchIC To facilitate NotchIC function. Molecular and cellular biology 210 10713164
2012 SKIP is a component of the spliceosome linking alternative splicing and the circadian clock in Arabidopsis. The Plant cell 182 22942380
2014 Necessary and sufficient role for a mitosis skip in senescence induction. Molecular cell 153 24910096
2018 CRISPR-SKIP: programmable gene splicing with single base editors. Genome biology 150 30107853
2008 Structure and function of Salmonella SifA indicate that its interactions with SKIP, SseJ, and RhoA family GTPases induce endosomal tubulation. Cell host & microbe 143 18996344
2015 SKIP Confers Osmotic Tolerance during Salt Stress by Controlling Alternative Gene Splicing in Arabidopsis. Molecular plant 128 25617718
1998 Isolation and characterization of a novel coactivator protein, NCoA-62, involved in vitamin D-mediated transcription. The Journal of biological chemistry 114 9632709
2000 A role for SKIP in EBNA2 activation of CBF1-repressed promoters. Journal of virology 97 10644367
1998 The Ski oncoprotein interacts with Skip, the human homolog of Drosophila Bx42. Oncogene 94 9569025
2005 A human splicing factor, SKIP, associates with P-TEFb and enhances transcription elongation by HIV-1 Tat. Genes & development 93 15905409
2004 MAGE-A1 interacts with adaptor SKIP and the deacetylase HDAC1 to repress transcription. Nucleic acids research 90 15316101
2002 Order of intron removal influences multiple splice outcomes, including a two-exon skip, in a COL5A1 acceptor-site mutation that results in abnormal pro-alpha1(V) N-propeptides and Ehlers-Danlos syndrome type I. American journal of human genetics 90 12145749
2003 SKIP negatively regulates insulin-induced GLUT4 translocation and membrane ruffle formation. Molecular and cellular biology 89 12556481
2021 To skip or not to skip: choosing repriming to tolerate DNA damage. Molecular cell 87 33515486
2003 Identification of a novel domain in two mammalian inositol-polyphosphate 5-phosphatases that mediates membrane ruffle localization. The inositol 5-phosphatase skip localizes to the endoplasmic reticulum and translocates to membrane ruffles following epidermal growth factor stimulation. The Journal of biological chemistry 84 12536145
2006 Relevance of skip metastases for squamous cell carcinoma of the oral tongue and the floor of the mouth. Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery 78 16500445
2016 Sexual divergence in microtubule function: the novel intranasal microtubule targeting SKIP normalizes axonal transport and enhances memory. Molecular psychiatry 76 26782054
2003 Role of skip metastasis to mediastinal lymph nodes in non-small cell lung cancer. Journal of surgical oncology 75 12672010
1996 Skip metastasis to the mediastinal lymph nodes in non-small cell lung cancer. The Annals of thoracic surgery 75 8823083
2000 The importance of skip lesions in temporal arteritis. Journal of clinical pathology 73 10767830
2004 Transcriptional coregulator SNW/SKIP: the concealed tie of dissimilar pathways. Cellular and molecular life sciences : CMLS 71 15052407
2020 SkipGNN: predicting molecular interactions with skip-graph networks. Scientific reports 68 33273494
2009 SKIP interacts with c-Myc and Menin to promote HIV-1 Tat transactivation. Molecular cell 64 19818711
2001 Ternary complexes and cooperative interplay between NCoA-62/Ski-interacting protein and steroid receptor coactivators in vitamin D receptor-mediated transcription. The Journal of biological chemistry 60 11514567
2004 To slip or skip, visualizing frameshift mutation dynamics for error-prone DNA polymerases. The Journal of biological chemistry 54 15339923
2012 Regulation of insulin signaling and glucose transporter 4 (GLUT4) exocytosis by phosphatidylinositol 3,4,5-trisphosphate (PIP3) phosphatase, skeletal muscle, and kidney enriched inositol polyphosphate phosphatase (SKIP). The Journal of biological chemistry 52 22247557
2008 Increased insulin action in SKIP heterozygous knockout mice. Molecular and cellular biology 51 18573875
2014 SNW1 enables sister chromatid cohesion by mediating the splicing of sororin and APC2 pre-mRNAs. The EMBO journal 49 25257309
2009 Reciprocal roles of SIRT1 and SKIP in the regulation of RAR activity: implication in the retinoic acid-induced neuronal differentiation of P19 cells. Nucleic acids research 48 19934264
2020 ARL8 Relieves SKIP Autoinhibition to Enable Coupling of Lysosomes to Kinesin-1. Current biology : CB 47 33232665
2009 Interaction between the SifA virulence factor and its host target SKIP is essential for Salmonella pathogenesis. The Journal of biological chemistry 47 19801640
2015 Skip residues modulate the structural properties of the myosin rod and guide thick filament assembly. Proceedings of the National Academy of Sciences of the United States of America 46 26150528
2015 MC1R gene variants and non-melanoma skin cancer: a pooled-analysis from the M-SKIP project. British journal of cancer 45 26103569
2002 Skip interacts with the retinoblastoma tumor suppressor and inhibits its transcriptional repression activity. Nucleic acids research 44 12466551
2019 SKIP regulates environmental fitness and floral transition by forming two distinct complexes in Arabidopsis. The New phytologist 41 31209881
2015 Rab1A regulates anterograde melanosome transport by recruiting kinesin-1 to melanosomes through interaction with SKIP. Scientific reports 41 25649263
2007 Skip mediastinal nodal metastases in the IIIa/N2 non-small cell lung cancer. Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 41 17975493
2014 Inhibition of SNW1 association with spliceosomal proteins promotes apoptosis in breast cancer cells. Cancer medicine 38 25450007
2011 SNW1 is a critical regulator of spatial BMP activity, neural plate border formation, and neural crest specification in vertebrate embryos. PLoS biology 38 21358802
1994 Skip mediastinal lymph node metastasis in non-small cell lung cancer. Journal of surgical oncology 38 7967601
2006 Solution structure of human peptidyl prolyl isomerase-like protein 1 and insights into its interaction with SKIP. The Journal of biological chemistry 37 16595688
2013 Supraomohyoid neck dissection in the management of oral squamous cell carcinoma: special consideration for skip metastases at level IV or V. Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons 36 24480771
2015 Rice cyclophilin OsCYP18-2 is translocated to the nucleus by an interaction with SKIP and enhances drought tolerance in rice and Arabidopsis. Plant, cell & environment 35 25847193
2003 Interactions of SKIP/NCoA-62, TFIIB, and retinoid X receptor with vitamin D receptor helix H10 residues. The Journal of biological chemistry 35 12529369
2013 Nodal skip metastasis is not a predictor of survival in thoracic esophageal squamous cell carcinoma. Annals of surgical oncology 33 23686016
2010 Xenopus skip modulates Wnt/beta-catenin signaling and functions in neural crest induction. The Journal of biological chemistry 32 20103590
2009 A large intrinsically disordered region in SKIP and its disorder-order transition induced by PPIL1 binding revealed by NMR. The Journal of biological chemistry 32 20007319
2001 The HPV-16 E7 oncoprotein binds Skip and suppresses its transcriptional activity. Oncogene 32 11753645
2015 Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes. PLoS genetics 31 26393790
2009 Prp45 affects Prp22 partition in spliceosomal complexes and splicing efficiency of non-consensus substrates. Journal of cellular biochemistry 31 19016306
2004 Emerging insights into the coactivator role of NCoA62/SKIP in Vitamin D-mediated transcription. The Journal of steroid biochemistry and molecular biology 31 15225769
2018 Role of Skip Mediastinal Lymph Node Metastasis for Patients With Resectable Non-small-cell Lung Cancer: A Propensity Score Matching Analysis. Clinical lung cancer 30 30665872
2017 Nodal Skip Metastasis in Esophageal Squamous Cell Carcinoma Patients Undergoing Three-Field Lymphadenectomy. The Annals of thoracic surgery 30 28669504
2004 Skip metastasis in nonsmall cell lung carcinoma: predictive markers and isolated tumor cells in N1 lymph nodes. Cancer 30 15112272
2021 CircRNA circ_0124554 blocked the ubiquitination of AKT promoting the skip lymphovascular invasion on hepatic metastasis in colorectal cancer. Cell death & disease 29 33723214
2019 Assessment of the Rate of Skip Metastasis to Neck Level IV in Patients With Clinically Node-Negative Neck Oral Cavity Squamous Cell Carcinoma: A Systematic Review and Meta-analysis. JAMA otolaryngology-- head & neck surgery 29 31070693
2019 SNW1, a Novel Transcriptional Regulator of the NF-κB Pathway. Molecular and cellular biology 28 30397075
2016 The SNW Domain of SKIP Is Required for Its Integration into the Spliceosome and Its Interaction with the Paf1 Complex in Arabidopsis. Molecular plant 28 27130079
2012 Regulation of insulin signaling by the phosphatidylinositol 3,4,5-triphosphate phosphatase SKIP through the scaffolding function of Pak1. Molecular and cellular biology 28 22751929
2001 Ski interacts with the evolutionarily conserved SNW domain of Skip. Nucleic acids research 28 11522815
2018 We skip to work: alternative splicing in normal and malignant myelopoiesis. Leukemia 27 29467484
2020 PTC located in the upper pole is more prone to lateral lymph node metastasis and skip metastasis. World journal of surgical oncology 26 32723382
2017 Adoption of the 2A Ribosomal Skip Principle to Tobacco Mosaic Virus for Peptide Display. Frontiers in plant science 26 28702043
2008 Selective neck dissection for N0 and N1 oral cavity and oropharyngeal cancer: are skip metastases a real danger? Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery 26 18983378
2017 Systemic Delivery of Morpholinos to Skip Multiple Exons in a Dog Model of Duchenne Muscular Dystrophy. Methods in molecular biology (Clifton, N.J.) 25 28364245
2017 Molecular Evidence for Monoclonal Skip Progression in Main Duct Intraductal Papillary Mucinous Neoplasms of the Pancreas. Annals of surgery 25 28398963
2014 Differential SKIP expression in PTEN-deficient glioblastoma regulates cellular proliferation and migration. Oncogene 24 25241900
2012 Isolated skip nodal metastasis is rare in T1 and T2 oral tongue squamous cell carcinoma. Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery 24 22371346
1992 The Drosophila nuclear protein Bx42, which is found in many puffs on polytene chromosomes, is highly charged. Chromosoma 24 1424996
2010 The crystal structure of PPIL1 bound to cyclosporine A suggests a binding mode for a linear epitope of the SKIP protein. PloS one 23 20368803
2002 Inducible RNA interference uncovers the Drosophila protein Bx42 as an essential nuclear cofactor involved in Notch signal transduction. Mechanisms of development 23 12204255
2001 Cyclophilins of a novel subfamily interact with SNW/SKIP coregulator in Dictyostelium discoideum and Schizosaccharomyces pombe. Biochimica et biophysica acta 23 11690648
2001 The fission yeast ortholog of the coregulator SKIP interacts with the small subunit of U2AF. Biochemical and biophysical research communications 22 11414703
2024 Effective multi-modal clustering method via skip aggregation network for parallel scRNA-seq and scATAC-seq data. Briefings in bioinformatics 21 38493338
2015 Phosphatidylinositol 3,4,5-Trisphosphate Phosphatase SKIP Links Endoplasmic Reticulum Stress in Skeletal Muscle to Insulin Resistance. Molecular and cellular biology 21 26483413
2004 Skip mediastinal nodal metastases in non-small cell lung cancer. European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 21 15145018
1996 The homolog of chromatin binding protein Bx42 identified in Dictyostelium. Gene 21 8973337
2020 Clinical Phenotypes of DMD Exon 51 Skip Equivalent Deletions: A Systematic Review. Journal of neuromuscular diseases 20 32417793
2017 Epidermal growth factor receptor mutations are linked to skip N2 lymph node metastasis in resected non-small-cell lung cancer adenocarcinomas. European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 20 28329143
2022 SKIP Regulates ABA Signaling through Alternative Splicing in Arabidopsis. Plant & cell physiology 19 35134199
2018 Antisense PMO cocktails effectively skip dystrophin exons 45-55 in myotubes transdifferentiated from DMD patient fibroblasts. PloS one 19 29771942
2015 Regulation of insulin signaling in skeletal muscle by PIP3 phosphatase, SKIP, and endoplasmic reticulum molecular chaperone glucose-regulated protein 78. Biochimica et biophysica acta 19 26376412
2024 To modulate or to skip: De-escalating PARP inhibitor maintenance therapy in ovarian cancer using adaptive therapy. Cell systems 18 38772367
2017 Nodal skip metastasis in thoracic esophageal squamous cell carcinoma: a cohort study. BMC surgery 18 28464907
2013 Sialyl Lewis X as a predictor of skip N2 metastasis in clinical stage IA non-small cell lung cancer. World journal of surgical oncology 18 24313932
2004 The human Ski-interacting protein functionally substitutes for the yeast PRP45 gene. Biochemical and biophysical research communications 18 15194481
2021 A Specific Predicting Model for Screening Skip Metastasis From Patients With Negative Central Lymph Nodes Metastasis in Papillary Thyroid Cancer. Frontiers in endocrinology 17 34659126
2020 Mesenteric Neural Crest Cells Are the Embryological Basis of Skip Segment Hirschsprung's Disease. Cellular and molecular gastroenterology and hepatology 17 33340715
2019 SNW1 regulates Notch signaling in neuroblastoma through interacting with RBPJ. Biochemical and biophysical research communications 17 30642633
2019 Survival benefit of skip metastases in surgically resected N2 non-small cell lung cancer: A multicenter observational study of a large cohort of the Chinese patients. European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology 17 31902592
2014 Clinical significance of ureteric 'skip lesions' at the time of radical cystectomy: the M.D. Anderson experience and literature review. BJU international 17 24053608
2008 Lobe-specific skip nodal metastasis in non-small cell lung cancer patients. Annals of thoracic and cardiovascular surgery : official journal of the Association of Thoracic and Cardiovascular Surgeons of Asia 17 18292733
2020 The incidence of skip metastases on whole bone MRI in high-grade bone sarcomas. Skeletal radiology 16 31919587
2018 Protein classification using modified n-grams and skip-grams. Bioinformatics (Oxford, England) 16 29309523
2002 Functional mapping of Saccharomyces cerevisiae Prp45 identifies the SNW domain as essential for viability. Journal of biochemistry 16 12359070
2019 Lipid phosphatases SKIP and SHIP2 regulate fibronectin-dependent cell migration in glioblastoma. The FEBS journal 15 30695232
2014 Venous thromboembolism in colorectal surgery: skip SCIP or comply? Techniques in coloproctology 15 24562596
2005 Echinococcus multilocularis: cloning and characterization of a member of the SNW/SKIP family of transcriptional coregulators. Experimental parasitology 15 15936017