Affinage

SNRPG

Small nuclear ribonucleoprotein G · UniProt P62308

Length
76 aa
Mass
8.5 kDa
Annotated
2026-06-10
33 papers in source corpus 6 papers cited in narrative 6 extracted findings
Cross-family judge vs UniProt: tie faithfulness: 4/4 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

SNRPG (SmG) is an Sm-domain spliceosome-associated protein that participates in alternative splicing regulation and has been repeatedly implicated as a driver of cancer cell proliferation and metastasis (PMID:32296580, PMID:41526677). In germ cells, SNRPG physically associates with PCID2, hnRNPH1, and SF3B1 within a PCID2-containing splicing complex that modulates alternative splicing during spermatogonial differentiation (PMID:41526677). Beyond its splicing-complex role, SNRPG operates in oncogenic signaling through two distinct routes: in glioblastoma it acts upstream of Myc in a p53-dependent pathway, where its loss arrests the cell cycle, lowers Myc protein, and sensitizes temozolomide-resistant cells to chemotherapy (PMID:32296580); in NSCLC it directly binds the transcription factor WT1 and destabilizes it, preventing WT1 from activating CDK14 transcription and thereby restraining epithelial-mesenchymal transition and metastasis downstream of 5-HT signaling (PMID:41016985). SNRPG also engages in direct, high-affinity physical interaction with the RING finger domain of RBBP6 (KD ~3 nM) (PMID:34956492). A unifying biochemical mechanism connecting its splicing function to its transcription-factor-destabilizing activity has not been characterized in the available corpus.

Mechanistic history

Synthesis pass · year-by-year structured walk · 5 steps
  1. 2020 Medium

    Established that SNRPG is not merely a constitutive splicing factor but a node controlling proliferation and chemoresistance, placing it upstream of Myc in a p53-dependent pathway.

    Evidence shRNA knockdown with expression profiling, Western blot pathway validation, and xenograft in glioblastoma cells

    PMID:32296580

    Open questions at the time
    • Mechanism by which SNRPG controls Myc/p53 levels is not defined
    • Whether the effect requires SNRPG's splicing function is untested
    • Single lab, single tumor type
  2. 2022 Medium

    Quantified a direct, high-affinity physical interaction between SNRPG and the RBBP6 RING finger domain, linking SNRPG to a specific binding partner in vitro.

    Evidence Microscale thermophoresis with purified proteins

    PMID:34956492

    Open questions at the time
    • Functional consequence of the SNRPG-RBBP6 interaction not established
    • Interaction shown in vitro only, not validated in cells
    • No structural detail of the interface
  3. 2022 Low

    Detected a direct binding of SNRPG to the HSP70.14 chaperone, hinting at a protein quality-control connection within cancer cell networks.

    Evidence Microscale thermophoresis binding assay

    PMID:36247303

    Open questions at the time
    • Single in vitro binding assay with no functional validation
    • Cellular relevance of the interaction unknown
    • Not independently confirmed
  4. 2025 Medium

    Defined a 5-HT/SNRPG/WT1/CDK14 axis, revealing that SNRPG acts as a metastasis suppressor in NSCLC by directly binding and destabilizing the WT1 transcription factor.

    Evidence RNA-seq, migration/invasion assays, direct SNRPG-WT1 binding assay, and CDK14 promoter binding assay in NSCLC cells

    PMID:41016985

    Open questions at the time
    • Molecular basis of WT1 destabilization (e.g. degradation route) not defined
    • Reconciliation with the proliferation-promoting role in glioblastoma unresolved
    • Single lab
  5. 2026 Medium

    Placed SNRPG in a defined PCID2-containing splicing complex required for spermatogonial differentiation, connecting its canonical Sm/splicing identity to a tissue-specific developmental function.

    Evidence Reciprocal Co-IP, IP-MS, RNA-seq, and PCR validation of splicing targets in germ cells

    PMID:41526677

    Open questions at the time
    • Direct vs indirect nature of each interaction within the complex not dissected
    • Specific splicing events driving differentiation only partially mapped
    • Single lab

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unknown how SNRPG's role as a core splicing-complex component mechanistically relates to its context-dependent regulation of transcription factors (Myc, WT1) and its opposing pro- versus anti-tumor effects across cancer types.
  • No unifying biochemical mechanism linking splicing function to transcription-factor destabilization
  • No structural model of SNRPG within its reported complexes
  • Tissue-specific opposing roles not reconciled

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 1 GO:0140096 catalytic activity, acting on a protein 1
Pathway
R-HSA-8953854 Metabolism of RNA 1
Partners
HNRNPH1HSPA14PCID2RBBP6SF3B1WT1
Complex memberships
PCID2-containing splicing complex

Evidence

Reading pass · 6 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2020 SNRPG knockdown via shRNA in glioblastoma cells caused cell cycle arrest and reduced Myc protein levels; the effect of SNRPG loss on p53 levels and cell cycle progression was Myc-dependent, placing SNRPG upstream of Myc in a p53-dependent signaling pathway. SNRPG was also elevated in TMZ-resistant GBM cells, and its downregulation sensitized resistant cells to temozolomide via the p53 signaling pathway. shRNA knockdown, gene expression profiling, Western blot, xenograft tumor model Cancer biology & medicine Medium 32296580
2022 SNRPG binds directly to the RING finger domain of RBBP6 with a strong binding affinity (KD ~3.16 nM), as measured by microscale thermophoresis (MST), confirming a physical protein-protein interaction between these two spliceosome-associated oncoproteins. Microscale thermophoresis (MST)-based binding assay with purified proteins American journal of translational research Medium 34956492
2022 SNRPG binds directly to HSP70.14 (heat shock protein 70.14) with a binding affinity of KD ~247 nM, as measured by microscale thermophoresis, revealing a physical interaction between SNRPG and a protein quality control chaperone in cancer cell networks. Microscale thermophoresis (MST)-based binding assay American journal of translational research Low 36247303
2025 5-HT inhibits SNRPG expression in NSCLC cells; SNRPG in turn directly binds to WT1 protein and negatively regulates its protein levels, preventing WT1 from binding the CDK14 promoter. Loss of SNRPG leads to WT1 accumulation, CDK14 transcriptional activation, and promotion of EMT and metastasis. This defines a 5-HT/SNRPG/WT1/CDK14 signaling axis. RNA-seq, functional migration/invasion assays, RNA-seq for downstream target identification, direct binding assay (SNRPG–WT1 interaction), promoter binding assay Molecular biomedicine Medium 41016985
2026 Co-immunoprecipitation followed by mass spectrometry (IP-MS) and co-IP in germ cells showed that SNRPG physically interacts with PCID2, hnRNPH1, and SF3B1 to modulate alternative splicing during spermatogenesis, placing SNRPG in a PCID2-containing splicing complex essential for spermatogonial differentiation. Co-immunoprecipitation (Co-IP), IP-MS, RNA sequencing, PCR validation of splicing targets Cellular and molecular life sciences : CMLS Medium 41526677
1997 Antisense RNA-mediated suppression of CaSm (an Sm-like protein with ~60% similarity to Sm-G/SNRPG) reduced the ability of pancreatic cancer cells to form colonies in soft agar, establishing that CaSm expression is required for maintenance of the transformed state. Note: CaSm is a distinct Sm-like protein, not SNRPG itself, but this paper identifies SNRPG as the closest human paralog for context. Antisense RNA transfection, soft-agar colony formation assay Cancer research Low 9230209

Source papers

Stage 0 corpus · 33 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2020 The Predicted Key Molecules, Functions, and Pathways That Bridge Mild Cognitive Impairment (MCI) and Alzheimer's Disease (AD). Frontiers in neurology 66 32308643
2022 Identification of diagnostic genes for both Alzheimer's disease and Metabolic syndrome by the machine learning algorithm. Frontiers in immunology 62 36405716
2021 m6A Modification Mediates Mucosal Immune Microenvironment and Therapeutic Response in Inflammatory Bowel Disease. Frontiers in cell and developmental biology 38 34422815
2020 Downregulation of SNRPG induces cell cycle arrest and sensitizes human glioblastoma cells to temozolomide by targeting Myc through a p53-dependent signaling pathway. Cancer biology & medicine 37 32296580
1997 CaSm: an Sm-like protein that contributes to the transformed state in cancer cells. Cancer research 37 9230209
2021 N6-methyladenosine (m6A) modification and its clinical relevance in cognitive dysfunctions. Aging 36 34461609
2024 Identification and validation of extracellular vesicle reference genes for the normalization of RT-qPCR data. Journal of extracellular vesicles 32 38545822
2021 Identification of potential biomarkers for pathogenesis of Alzheimer's disease. Hereditas 25 34225819
2019 The oncogenic potential of small nuclear ribonucleoprotein polypeptide G: a comprehensive and perspective view. American journal of translational research 20 31814883
2025 Single-cell RNA transcriptome uncovers distinct developmental trajectories in the embryonic skeletal muscle of Daheng broiler and Tibetan chicken. BMC genomics 13 39994525
2022 Identification of potential blood biomarkers for early diagnosis of schizophrenia through RNA sequencing analysis. Journal of psychiatric research 13 35016150
2021 Identification of COL6A1 as the Key Gene Associated with Antivascular Endothelial Growth Factor Therapy in Glioblastoma Multiforme. Genetic testing and molecular biomarkers 11 33970702
2020 Clinical Significance, Cellular Function, and Potential Molecular Pathways of CCT7 in Endometrial Cancer. Frontiers in oncology 11 32983981
2023 A MACHINE LEARNING MODEL DERIVED FROM ANALYSIS OF TIME-COURSE GENE-EXPRESSION DATASETS REVEALS TEMPORALLY STABLE GENE MARKERS PREDICTIVE OF SEPSIS MORTALITY. Shock (Augusta, Ga.) 7 37752077
2023 Dye Degradation, Antimicrobial Activity, and Molecular Docking Analysis of Samarium-Grafted Carbon Nitride Doped-Bismuth Oxobromide Quantum Dots. Global challenges (Hoboken, NJ) 7 38094862
2022 The Underlying Molecular Basis and Mechanisms of Venous Thrombosis in Patients with Osteomyelitis: A Data-Driven Analysis. Genetics research 6 35711689
2022 An NADPH-auxotrophic Corynebacterium glutamicum recombinant strain and used it to construct L-leucine high-yielding strain. International microbiology : the official journal of the Spanish Society for Microbiology 6 35925494
2021 Fluctuation analysis to select for Samarium bio-uptaking microalgae clones the repurposing of a classical evolution experiment. Ecotoxicology and environmental safety 3 33721662
2025 Construction and differential analysis of testicular atlas between 10-week-old and 23-week-old ducks using single-cell RNA sequencing. Poultry science 2 40884870
2024 In-silico identification of therapeutic targets in pancreatic ductal adenocarcinoma using WGCNA and Trader. Scientific reports 2 39375436
2022 Molecular interaction between small nuclear ribonucleoprotein polypeptide G and heat shock protein 70.14: a microscale thermophoresis exposition towards developing anti-cancer drugs. American journal of translational research 2 36247303
2021 Microscale thermophoresis analysis of the molecular interaction between small nuclear ribonucleoprotein polypeptide G and the RING finger domain of RBBP6 towards anti-cancer drug discovery. American journal of translational research 2 34956492
2016 Ectopic Gastric and Intestinal Phenotypes, Neuroendocrine Cell Differentiation, and SOX2 Expression Correlated With Early Tumor Progression in Colorectal Laterally Spreading Tumors. Clinical colorectal cancer 2 27816419
2025 A Validated Proteomic Signature of Basal-like Triple-Negative Breast Cancer Subtypes Obtained from Publicly Available Data. Cancers 1 40867231
2025 Enhancement of direct interspecies electron transfer and methane production by co-culture of dual Methanosarcina species and Geobacter metallireducens. Frontiers in microbiology 1 40895471
2025 5-Hydroxytryptamine promotes non-small cell lung cancer metastasis via the SNRPG/WT1/CDK14 Axis. Molecular biomedicine 1 41016985
2024 Phosphoproteomics analysis of serum from dogs affected with pulmonary hypertension secondary to degenerative mitral valve disease. PeerJ 1 38708342
2024 From Diabetes to Dementia: Identifying Key Genes in the Progression of Cognitive Impairment. Brain sciences 1 39452046
2023 Efficient Samarium-Grafted-C3N4-Doped α-MoO3 Used as a Dye Degrader and Antibacterial Agent: In Silico Molecular Docking Study. ACS omega 1 37779977
2020 Variation in mouse pelvic morphology maps to locations enriched in Sox9 Class II and Pitx1 regulatory features. Journal of experimental zoology. Part B, Molecular and developmental evolution 1 32017444
2026 PCID2 is essential for spermatogonial differentiation by regulating alternative splicing. Cellular and molecular life sciences : CMLS 0 41526677
2026 Comparison of Sertoli cells in duck testes pre- and post-sexual maturation. Poultry science 0 42190478
2024 Single-cell RNA-seq reveals diverse molecular signatures associated with Methotrexate resistance in primary central nervous system lymphoma cells. Journal of neuro-oncology 0 39636551

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